##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364583_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1358156 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.92243011848418 31.0 30.0 34.0 26.0 34.0 2 29.82322943756093 31.0 30.0 34.0 26.0 34.0 3 30.08470234641676 31.0 30.0 34.0 26.0 34.0 4 33.59702935450714 35.0 35.0 37.0 28.0 37.0 5 33.021444517419205 35.0 33.0 37.0 26.0 37.0 6 33.044527285525376 35.0 33.0 37.0 26.0 37.0 7 32.825362476769975 35.0 33.0 37.0 25.0 37.0 8 32.74607556127573 35.0 33.0 37.0 25.0 37.0 9 33.948183419283204 38.0 34.0 39.0 23.0 39.0 10-11 33.872622879845906 38.0 33.0 39.0 23.5 39.0 12-13 33.74566507823843 38.0 33.0 39.0 22.5 39.0 14-15 34.674428784322274 39.0 33.0 41.0 22.5 41.0 16-17 34.69679587617328 39.0 33.0 41.0 22.5 41.0 18-19 34.74180801027275 39.0 33.5 41.0 23.0 41.0 20-21 34.77227137383335 39.0 34.0 41.0 23.0 41.0 22-23 34.738054391395394 39.0 34.0 41.0 23.0 41.0 24-25 34.69026017629786 39.0 34.0 41.0 23.0 41.0 26-27 34.56947287351379 39.0 33.5 41.0 23.0 41.0 28-29 34.412842118283905 39.0 33.0 41.0 22.5 41.0 30-31 34.22911027893703 38.0 33.0 40.5 21.5 41.0 32-33 33.990471271341434 38.0 33.0 40.0 19.5 41.0 34-35 33.783062475886425 38.0 33.0 40.0 18.0 41.0 36-37 33.562422137074094 38.0 33.0 40.0 16.0 41.0 38-39 33.38159055366246 38.0 32.0 40.0 15.5 41.0 40-41 33.39565116231125 38.0 33.0 40.0 15.0 41.0 42-43 33.24147115647982 38.0 32.0 40.0 11.5 41.0 44-45 33.03525846809939 38.0 32.0 40.0 8.5 41.0 46-47 32.72575204910187 38.0 31.5 40.0 4.5 41.0 48-49 32.38560739708841 38.0 31.0 40.0 2.0 41.0 50-51 32.19687539575719 38.0 31.0 40.0 2.0 41.0 52-53 32.01248126135731 37.5 30.5 40.0 2.0 41.0 54-55 31.820818079808213 37.0 30.0 40.0 2.0 41.0 56-57 31.603626166655374 37.0 30.0 40.0 2.0 41.0 58-59 31.391893861971674 36.5 30.0 40.0 2.0 41.0 60-61 31.155559817870703 36.0 29.5 40.0 2.0 41.0 62-63 30.983157310353157 36.0 29.5 39.5 2.0 41.0 64-65 30.8469052892304 35.5 29.5 39.0 2.0 41.0 66-67 30.551093541537202 35.0 29.0 39.0 2.0 41.0 68-69 30.270991697566405 35.0 29.0 39.0 2.0 40.5 70-71 29.95567482675039 35.0 29.0 38.0 2.0 40.0 72-73 29.670630251605854 35.0 29.0 37.0 2.0 40.0 74-75 29.32818763087598 35.0 29.0 37.0 2.0 39.0 76-77 29.01672856431809 34.5 28.5 36.0 2.0 39.0 78-79 28.731200981330566 34.0 28.0 36.0 2.0 38.5 80-81 28.452043800564883 34.0 28.0 35.5 2.0 37.0 82-83 28.168535867750098 34.0 28.0 35.0 2.0 37.0 84-85 27.94195585779542 34.0 27.0 35.0 2.0 36.5 86-87 27.731126983939987 34.0 27.0 35.0 2.0 36.0 88-89 27.55561180011722 34.0 27.0 35.0 2.0 36.0 90-91 27.413378139182832 34.0 26.0 35.0 2.0 35.5 92-93 27.290257157498843 34.0 26.0 35.0 2.0 35.0 94-95 27.176804063745255 34.0 26.0 35.0 2.0 35.0 96-97 27.0583942492615 34.0 26.0 35.0 2.0 35.0 98-99 26.973238714845717 34.0 26.0 35.0 2.0 35.0 100-101 26.86879563172419 34.0 25.0 35.0 2.0 35.0 102-103 25.611746736015597 31.5 23.5 34.0 2.0 35.0 104-105 26.52781896924948 33.0 25.0 35.0 2.0 35.0 106-107 26.686581659249747 34.0 25.0 35.0 2.0 35.0 108-109 26.689642058791478 34.0 25.0 35.0 2.0 35.0 110-111 26.607937158912527 34.0 25.0 35.0 2.0 35.0 112-113 26.522902744603712 34.0 25.0 35.0 2.0 35.0 114-115 26.416446637941444 34.0 25.0 35.0 2.0 35.0 116-117 26.291878473459604 34.0 24.5 35.0 2.0 35.0 118-119 26.156735308756872 34.0 24.0 35.0 2.0 35.0 120-121 25.956410751047745 34.0 23.5 35.0 2.0 35.0 122-123 25.660659747481144 33.0 21.5 35.0 2.0 35.0 124-125 23.878583535322893 31.0 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7044763444517379 1101 2 0.917776857197147 1101 3 0.7163604371655587 1101 4 0.6590305618473593 1101 5 0.6330920020362214 1101 6 0.6633955431328786 1101 7 0.8062628565819523 1101 8 0.768697778947697 1101 9 1.0483074095914873 1101 10-11 0.9047365478746272 1101 12-13 0.7694600394752484 1101 14-15 1.0338990045404728 1101 16-17 0.9761401593533563 1101 18-19 0.9361503117152665 1101 20-21 1.0078091347536429 1101 22-23 0.8337863192260073 1101 24-25 0.9653702974436627 1101 26-27 1.090057162883916 1101 28-29 0.9843986475044986 1101 30-31 1.0503549567703203 1101 32-33 1.1183853200737133 1101 34-35 1.1997778790325668 1101 36-37 1.133116158266219 1101 38-39 0.9880945459436319 1101 40-41 0.8325999402029254 1101 42-43 1.039345512934716 1101 44-45 0.8907929916199535 1101 46-47 1.033304193271512 1101 48-49 1.1426842081426827 1101 50-51 1.21463515532092 1101 52-53 1.281943597479973 1101 54-55 1.2465357100149603 1101 56-57 1.2965321180004814 1101 58-59 1.3009009038279231 1101 60-61 1.329013154260732 1101 62-63 1.3890106321923135 1101 64-65 1.270584401071286 1101 66-67 1.3474627875970207 1101 68-69 1.244088130569036 1101 70-71 1.153712657165947 1101 72-73 1.1680963816667926 1101 74-75 1.2805594963502784 1101 76-77 1.2907331769351202 1101 78-79 1.219400248494857 1101 80-81 1.1232316359038101 1101 82-83 1.3207781180727416 1101 84-85 1.3554917280922218 1101 86-87 1.388373923399211 1101 88-89 1.3367454736849709 1101 90-91 1.311842615354056 1101 92-93 1.2460604533115855 1101 94-95 1.224594303622137 1101 96-97 1.2930202675695135 1101 98-99 1.4224313954651286 1101 100-101 1.3008563508070985 1101 102-103 1.2174114454489313 1101 104-105 1.3476637668346214 1101 106-107 1.2840834520697832 1101 108-109 1.3238594228620144 1101 110-111 1.1542844218495922 1101 112-113 1.3066039588782026 1101 114-115 1.3694686849466464 1101 116-117 1.2575795819742837 1101 118-119 1.3187424270411512 1101 120-121 1.1882356392456153 1101 122-123 1.256945811557209 1101 124-125 1.400771777536395 1102 1 0.4699861311726501 1102 2 -0.11021313745254346 1102 3 0.38708012168741135 1102 4 0.5962825668554643 1102 5 0.6587762034142699 1102 6 0.527796430686756 1102 7 0.5047416848370148 1102 8 0.4589501668967344 1102 9 1.0251489888290237 1102 10-11 0.9532247459610161 1102 12-13 1.0878007374054732 1102 14-15 1.218131615355702 1102 16-17 1.099361588836551 1102 18-19 1.066559352380338 1102 20-21 1.0383371315380217 1102 22-23 1.1735187494343506 1102 24-25 1.1959971527381654 1102 26-27 1.215964610677652 1102 28-29 0.8696969747859171 1102 30-31 1.0930610183722038 1102 32-33 1.2943588998652942 1102 34-35 1.4144461462655826 1102 36-37 1.097125142440703 1102 38-39 0.5560828567395788 1102 40-41 0.34946563657150165 1102 42-43 0.5747471799499237 1102 44-45 0.15423869955822767 1102 46-47 0.4225813039957558 1102 48-49 0.13040565527917636 1102 50-51 0.17798417746359974 1102 52-53 -0.10763497738226491 1102 54-55 0.04982085435110051 1102 56-57 -0.05257693325657442 1102 58-59 0.08883174632513402 1102 60-61 -0.08014429421197988 1102 62-63 0.10074709278606164 1102 64-65 0.03316781293919391 1102 66-67 -0.030374981242452748 1102 68-69 0.1607998357968583 1102 70-71 -0.3593114083631228 1102 72-73 -0.1264856831181227 1102 74-75 0.020940481839261338 1102 76-77 -0.29025339840511677 1102 78-79 -0.20442166496049197 1102 80-81 -0.2512316357153779 1102 82-83 -0.5349889452048515 1102 84-85 -0.318371602733432 1102 86-87 -0.15372242019386206 1102 88-89 -0.2731517840777329 1102 90-91 -0.28631383638588304 1102 92-93 -0.17669139824855762 1102 94-95 0.05889087372069568 1102 96-97 -0.23691430656485934 1102 98-99 -0.25470187069511496 1102 100-101 -0.14926083998167883 1102 102-103 -0.17056339451706393 1102 104-105 -0.01935388375486724 1102 106-107 0.10455748405757248 1102 108-109 0.17744019216590345 1102 110-111 0.30159446549399505 1102 112-113 0.4496391867280529 1102 114-115 0.4224978563548092 1102 116-117 0.3241841452179308 1102 118-119 0.5150268136431073 1102 120-121 0.7245960165374896 1102 122-123 0.5508258179667145 1102 124-125 0.7985401281473337 1103 1 0.5376308618351082 1103 2 0.11920757240701008 1103 3 0.44397109726962114 1103 4 0.494188286895735 1103 5 0.507137330062065 1103 6 0.5316580219651712 1103 7 0.567144774502033 1103 8 0.8555190786125095 1103 9 1.011215705033429 1103 10-11 0.9058571669131723 1103 12-13 0.9051822835462389 1103 14-15 1.0644209524687938 1103 16-17 1.0317360901824202 1103 18-19 1.027561494360576 1103 20-21 1.0702255163114671 1103 22-23 1.0851187618800253 1103 24-25 1.0457014575476151 1103 26-27 1.1074529647778846 1103 28-29 1.1272831445954878 1103 30-31 1.2227587426498872 1103 32-33 1.3019145067062041 1103 34-35 1.154110044258033 1103 36-37 1.4203254123258091 1103 38-39 1.3935132203323377 1103 40-41 1.2583151043348622 1103 42-43 1.325649430633888 1103 44-45 1.4282025781716783 1103 46-47 1.5420980685496701 1103 48-49 1.489035694402233 1103 50-51 1.4159389991485796 1103 52-53 1.3769600614455655 1103 54-55 1.4346540760897852 1103 56-57 1.466522533465561 1103 58-59 1.5809793295721661 1103 60-61 1.6283273687099573 1103 62-63 1.536564981101744 1103 64-65 1.427052695772172 1103 66-67 1.3407717878903966 1103 68-69 1.357362230679236 1103 70-71 1.1425645086790581 1103 72-73 1.031845194153 1103 74-75 1.2507116552392112 1103 76-77 1.3107930433425992 1103 78-79 1.1795594685618305 1103 80-81 1.1400555785269937 1103 82-83 1.2745565987633185 1103 84-85 1.338374410054751 1103 86-87 1.4083175887533095 1103 88-89 1.46987600041696 1103 90-91 1.4519794441463993 1103 92-93 1.3487792530942393 1103 94-95 1.3418613345808659 1103 96-97 1.275100798768971 1103 98-99 1.414364509622871 1103 100-101 1.3264697129667375 1103 102-103 1.1090197829390043 1103 104-105 1.0925357357870098 1103 106-107 1.213718730850296 1103 108-109 1.1480535609351286 1103 110-111 1.0893979177987383 1103 112-113 0.9602340633700344 1103 114-115 1.0943369532882947 1103 116-117 1.285523766070522 1103 118-119 0.9572124566671434 1103 120-121 1.2205038861528017 1103 122-123 1.2540163958995905 1103 124-125 1.5464582326209957 1104 1 0.4177066156917739 1104 2 0.5977927260063218 1104 3 0.33628782435637206 1104 4 0.13377228141586528 1104 5 0.45022813258783856 1104 6 0.5984377155617082 1104 7 0.5956529596200468 1104 8 0.6162640698992448 1104 9 0.6829204134209519 1104 10-11 0.6546657630444841 1104 12-13 0.5110594073243249 1104 14-15 0.6087852900754456 1104 16-17 0.5735187166249673 1104 18-19 0.6434434835515717 1104 20-21 0.4935953782706406 1104 22-23 0.6516445827945745 1104 24-25 0.7312253445123815 1104 26-27 0.668658868084087 1104 28-29 0.8999858503504825 1104 30-31 0.7451754815141172 1104 32-33 0.8572892898542293 1104 34-35 1.0188145735633327 1104 36-37 0.9068814536543925 1104 38-39 1.0577787561765533 1104 40-41 0.9356300187038258 1104 42-43 0.972643672575586 1104 44-45 0.8130876232844955 1104 46-47 0.7640217435659054 1104 48-49 0.7816449916047503 1104 50-51 0.7556917504347069 1104 52-53 0.7486447642508907 1104 54-55 0.462819077890984 1104 56-57 0.5768726795547074 1104 58-59 0.6076284706112887 1104 60-61 0.807788273584567 1104 62-63 0.7451288223025969 1104 64-65 0.6049404658388831 1104 66-67 0.6893961390630352 1104 68-69 0.4591329090874261 1104 70-71 0.11328133772974525 1104 72-73 0.11016059506325249 1104 74-75 0.568319748188653 1104 76-77 0.632557941336028 1104 78-79 0.7280231066875835 1104 80-81 0.7161466260520051 1104 82-83 0.4994266028128358 1104 84-85 0.5405899360920827 1104 86-87 0.5808116879132363 1104 88-89 0.5550703945209357 1104 90-91 0.5949927365650929 1104 92-93 0.573213421245768 1104 94-95 0.5765234718730916 1104 96-97 0.6964097169947685 1104 98-99 0.7482789959000336 1104 100-101 0.5701311355434058 1104 102-103 0.541257211401188 1104 104-105 0.611289701467296 1104 106-107 0.6548079380000331 1104 108-109 0.7016054852163087 1104 110-111 0.6406571516540822 1104 112-113 0.7295347829947971 1104 114-115 0.6779812604901423 1104 116-117 0.8068933233363857 1104 118-119 0.6855526686479507 1104 120-121 0.7296867480066531 1104 122-123 0.7307687800325837 1104 124-125 1.1795071087013902 1105 1 0.16492515358369175 1105 2 -0.21588541625548885 1105 3 0.05466190165070017 1105 4 0.0739382725815858 1105 5 -0.11085612506788323 1105 6 0.182070304271285 1105 7 0.19620150327704522 1105 8 0.3140946224401162 1105 9 0.41503655167544196 1105 10-11 0.3440611826177076 1105 12-13 0.38924779437133594 1105 14-15 0.5351762736818131 1105 16-17 0.4068828536892468 1105 18-19 0.4242536622369357 1105 20-21 0.5158983891765416 1105 22-23 0.5930454400490106 1105 24-25 0.6139958343697671 1105 26-27 0.502523403147876 1105 28-29 0.2996554200679 1105 30-31 0.4830025166898295 1105 32-33 0.5514386626175565 1105 34-35 0.6276951629194372 1105 36-37 0.6272666510573259 1105 38-39 0.7009246122635133 1105 40-41 0.5074011429267387 1105 42-43 0.48877464896814615 1105 44-45 0.451857436301637 1105 46-47 0.3963962428522194 1105 48-49 0.41381825260403104 1105 50-51 0.15358484411578388 1105 52-53 0.2918925137226509 1105 54-55 0.21311622866448943 1105 56-57 0.14724581184414376 1105 58-59 0.059504394866713994 1105 60-61 -0.019272501755672522 1105 62-63 0.20163448992123278 1105 64-65 -0.008830225371585954 1105 66-67 0.21048265132227328 1105 68-69 0.16178434118540252 1105 70-71 0.2923886938214082 1105 72-73 0.34725396572535416 1105 74-75 0.3450420230514517 1105 76-77 0.3830571339721516 1105 78-79 0.22870591634601212 1105 80-81 0.0904656818369105 1105 82-83 0.17626787574085867 1105 84-85 0.19632483696414837 1105 86-87 0.31077412879016464 1105 88-89 0.34465963652645115 1105 90-91 0.19735701666582983 1105 92-93 0.19351557485130755 1105 94-95 0.16135155146748303 1105 96-97 0.21326505938340645 1105 98-99 0.048171913712849346 1105 100-101 -0.0427790410067459 1105 102-103 -0.28032708149706664 1105 104-105 -0.059025619298267884 1105 106-107 -0.09532505376009937 1105 108-109 -0.06389566010850345 1105 110-111 -0.24504983300198901 1105 112-113 -0.1628997365002931 1105 114-115 -0.06831224710123251 1105 116-117 -0.47541299830094275 1105 118-119 -0.17413476799872285 1105 120-121 -0.31496565159633505 1105 122-123 -0.21895953106045596 1105 124-125 0.17408366224833927 1106 1 -0.06583670957114052 1106 2 -0.5242620633664963 1106 3 0.09247887317077286 1106 4 -0.06479759033427257 1106 5 0.3205932340228017 1106 6 0.29062193583581575 1106 7 0.5205068063107277 1106 8 0.3612782154996168 1106 9 0.5030860806799282 1106 10-11 0.4257969279225975 1106 12-13 0.4006727251252471 1106 14-15 0.5497844472446047 1106 16-17 0.403457463759608 1106 18-19 0.561731839065132 1106 20-21 0.4426255859720811 1106 22-23 0.3973512454528745 1106 24-25 0.4297307960710768 1106 26-27 0.6777582201243249 1106 28-29 0.6419158792858823 1106 30-31 0.6090099860423166 1106 32-33 0.4390814595799597 1106 34-35 0.4087634131946345 1106 36-37 0.6008479658915462 1106 38-39 0.5710412630883042 1106 40-41 0.5161284162515614 1106 42-43 0.4444786280451325 1106 44-45 0.4087918701856168 1106 46-47 0.19508677038081146 1106 48-49 0.3704911722926454 1106 50-51 0.34003120189677816 1106 52-53 0.2891892222354997 1106 54-55 0.25173558395453455 1106 56-57 -0.11366882583779514 1106 58-59 0.23949928665406617 1106 60-61 0.2983346104914304 1106 62-63 0.23659851528670472 1106 64-65 0.3416766168398091 1106 66-67 0.14007711417000124 1106 68-69 0.26710437094261863 1106 70-71 0.23407724919492523 1106 72-73 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2216 92-93 -1.5078076144156398 2216 94-95 -1.4772865505742736 2216 96-97 -1.5572022863365085 2216 98-99 -1.570018710806096 2216 100-101 -1.6717457401665285 2216 102-103 -1.5246418921547829 2216 104-105 -1.5674376506510441 2216 106-107 -1.5627932488590481 2216 108-109 -1.7121575902260098 2216 110-111 -1.5718556561582666 2216 112-113 -1.5054066609752326 2216 114-115 -1.5984802264478901 2216 116-117 -1.6979527004020945 2216 118-119 -1.7280899327175092 2216 120-121 -1.728511886360696 2216 122-123 -1.645237633128339 2216 124-125 -2.0106973205448178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46636.0 3 15667.0 4 7361.0 5 5743.0 6 6333.0 7 7653.0 8 8543.0 9 9149.0 10 10275.0 11 11304.0 12 12569.0 13 14663.0 14 17456.0 15 15586.0 16 9930.0 17 8615.0 18 7919.0 19 7256.0 20 7145.0 21 7344.0 22 8346.0 23 10186.0 24 13185.0 25 17478.0 26 22167.0 27 24212.0 28 27276.0 29 32420.0 30 37370.0 31 43021.0 32 52654.0 33 65606.0 34 84464.0 35 116192.0 36 184630.0 37 279989.0 38 101396.0 39 417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05245791449278 19.21771552769304 13.56833362691173 23.161492930902455 2 15.50529946876461 20.643740957401768 39.653132374434435 24.197827199399185 3 19.51189701330333 26.171146760755025 29.052627238697177 25.264328987244472 4 12.340993833225113 17.029939113508604 36.34380341203151 34.28526364123477 5 12.62130418007946 39.426914139465566 33.55211036140178 14.399671319053187 6 31.441454442641348 37.159354300978684 17.172990437033743 14.226200819346232 7 28.12960192264647 33.399208322043286 20.36361815675695 18.107571598553303 8 26.828862408519143 35.69710272879695 18.83436138464042 18.639673478043488 9 27.118728931994802 15.753750454678661 19.393167818028395 37.73435279529814 10-11 25.19609438448121 27.369386377174195 26.518226672075997 20.9162925662686 12-13 26.127042843384707 24.67669398802494 27.580373683140962 21.615889485449387 14-15 22.895030390492984 25.498562387945412 26.178381701646718 25.428025519914883 16-17 21.842723847285193 29.11558164491837 26.654885957210254 22.38680855058618 18-19 21.601514438634972 26.847378779745927 30.143756806248607 21.407349975370494 20-21 22.604289997467117 25.89902631253424 30.251756819641034 21.24492687035761 22-23 22.163322917249566 25.891834222283745 30.162256765791263 21.782586094675427 24-25 21.93886416582484 26.089491928762232 30.310840580905285 21.66080332450764 26-27 21.662349538639155 26.38496608637005 30.272222042239626 21.680462332751173 28-29 21.52274849133678 26.242972088626047 30.556541369327235 21.677738050709934 30-31 21.60856337563579 26.073808899714024 30.468259905342244 21.849367819307943 32-33 21.540640397715727 26.224417519047886 30.328364341062443 21.906577742173948 34-35 21.929881398013187 26.20652561266894 30.16372935067842 21.69986363863945 36-37 22.01385555120325 25.948712813550134 30.073202194740517 21.9642294405061 38-39 21.85949184040714 26.32554728617331 30.074527521138954 21.74043335228059 40-41 21.915965470829565 26.07425067518017 30.23573875166034 21.77404510232992 42-43 21.889606201349476 26.145376525229796 29.77463560886967 22.190381664551055 44-45 22.060278789770837 26.357391934358056 29.54439696176286 22.037932314108247 46-47 22.114396284373814 26.692036849964218 28.935740813279182 22.25782605238279 48-49 22.374859736289498 26.87463737597154 28.39754785164591 22.352955036093057 50-51 22.290370178388933 27.05057445536448 28.280256465383946 22.37879890086264 52-53 22.538905692718654 27.13487994015415 28.047256721613717 22.278957645513476 54-55 22.197965476719904 27.00496113848483 28.220101372743635 22.576972012051634 56-57 22.223879014407466 27.292669025938736 28.10272995171003 22.380722007943767 58-59 22.359149031418923 26.91053579271456 28.149908717505806 22.580406458360713 60-61 22.396064958664542 27.054955395403766 28.399204509643962 22.14977513628773 62-63 22.218986625984055 26.984823540152973 28.488443153805605 22.30774668005737 64-65 22.080767807738823 26.703702272020262 28.497720248646598 22.717809671594317 66-67 22.056744586041663 27.344355140352068 28.05940554693275 22.53949472667352 68-69 22.16005457401601 27.014137924560185 28.23314414292031 22.5926633585035 70-71 22.00119132117371 27.061913358995582 28.269359337218997 22.667535982611717 72-73 22.30782030930173 27.01155095585485 28.076855677845547 22.60377305699787 74-75 22.069063521386955 27.170264353995645 27.986343096924397 22.774329027693003 76-77 22.211660516170454 27.036400825825606 28.09552069129025 22.65641796671369 78-79 22.204628923334287 27.207846521312725 27.915276301102377 22.672248254250615 80-81 21.991733629519786 27.421458620104428 27.919671819127956 22.66713593124783 82-83 22.129711167200234 27.429728249184926 27.771220684516358 22.669339899098485 84-85 22.127980879957825 27.26122772347212 27.658715199137657 22.9520761974324 86-87 22.115390279172644 27.030252783921725 27.996747059984273 22.857609876921355 88-89 22.124457760046003 26.95536798686733 27.856821777144397 23.063352475942274 90-91 21.99762030282236 27.248048088732073 27.985481785597532 22.76884982284804 92-93 22.05342035469254 27.133368283247457 27.940873286435654 22.87233807562435 94-95 21.962867299485477 26.91855722023096 27.930627998550978 23.187947481732586 96-97 22.152153431016462 26.976860976244645 27.829087785583607 23.041897807155287 98-99 21.923115296865433 26.901178375500745 27.683338137844938 23.492368189788884 100-101 21.657267041277233 26.92476335880111 28.07631717780773 23.341652422113928 102-103 22.06966651842645 26.77862484132898 27.87890345438963 23.27280518585494 104-105 21.95509941420573 26.681139721799262 27.97311207254542 23.390648791449582 106-107 21.858411347590838 26.808237893263463 27.959703667924884 23.373647091220814 108-109 21.846827610377602 26.969066881860403 27.537411019058194 23.6466944887038 110-111 21.65260163508523 26.935060087007713 27.859733292689974 23.552604985217084 112-113 21.73811403108332 26.93158959648229 27.771110240649822 23.559186131784568 114-115 21.540088178383044 26.975251738386458 27.687725121414626 23.796934961815875 116-117 21.418710369059223 26.825121709140927 27.799494314349747 23.956673607450103 118-119 21.48320958711665 26.917047820721624 27.601063500805505 23.99867909135622 120-121 21.294964823343605 26.99814820950635 27.791620187829725 23.915266779320323 122-123 21.422764946012336 26.906282950736625 27.640845070422536 24.030107032828507 124-125 21.19762932352601 26.878167183830588 27.650081314232533 24.274122178410867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 401.0 1 306.5 2 508.5 3 766.0 4 518.5 5 347.0 6 599.5 7 1475.0 8 2483.0 9 2812.0 10 2173.5 11 1371.5 12 1169.0 13 1193.5 14 1330.0 15 1520.0 16 1751.5 17 1984.5 18 2228.5 19 2501.0 20 2864.0 21 3356.0 22 3922.0 23 4528.5 24 5200.5 25 6311.5 26 8182.0 27 10182.0 28 11698.0 29 13483.5 30 16134.0 31 18980.0 32 21832.0 33 24698.0 34 27793.0 35 30314.0 36 33403.0 37 37066.0 38 38486.5 39 39020.5 40 39476.0 41 40727.5 42 42550.0 43 44249.0 44 45987.5 45 46635.5 46 46478.5 47 46710.5 48 49913.5 49 52015.5 50 50972.0 51 48977.5 52 46607.0 53 45913.5 54 44551.5 55 41115.5 56 37169.0 57 33563.5 58 31089.0 59 28457.5 60 24672.0 61 20242.5 62 16030.5 63 12332.0 64 9143.0 65 6890.5 66 5096.0 67 3963.5 68 2959.5 69 1959.0 70 1517.0 71 1265.0 72 1038.5 73 936.0 74 776.0 75 589.0 76 366.0 77 184.5 78 126.5 79 83.0 80 49.5 81 25.0 82 15.0 83 13.0 84 6.5 85 5.5 86 4.5 87 3.0 88 2.5 89 1.0 90 1.0 91 1.5 92 1.0 93 0.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.098295041217651 2 7.362924435779101E-5 3 0.0 4 0.10079843552581588 5 0.0 6 0.0 7 0.0020616188420181482 8 0.006258485770412235 9 0.003975979195320715 10-11 6.62663199220119E-4 12-13 0.0 14-15 7.362924435779101E-5 16-17 2.5770235525226853E-4 18-19 0.002135248086375939 20-21 0.00147258488715582 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.00809921687935701 58-59 6.994778213990146E-4 60-61 0.0 62-63 0.0 64-65 2.5770235525226853E-4 66-67 0.0 68-69 3.6814622178895505E-5 70-71 0.0 72-73 0.0 74-75 0.0011044386653668652 76-77 0.0 78-79 0.0 80-81 1.840731108944775E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012148825319035516 90-91 0.0 92-93 0.0016198433758714023 94-95 0.0 96-97 0.00924047016690277 98-99 0.004233681550572983 100-101 0.00254020893034379 102-103 0.0 104-105 0.0 106-107 0.0012885117762613425 108-109 0.0 110-111 3.6814622178895505E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.099216879357011E-4 122-123 0.005006788616329788 124-125 0.07915143768462533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1358156.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.42418458421108 #Duplication Level Percentage of deduplicated Percentage of total 1 80.57144767360022 47.07321131099524 2 10.876466740369763 12.708974009267912 3 3.5730722882504966 6.262615047044292 4 1.5418831110945033 3.603330539594516 5 0.8421084126017501 2.459974866888081 6 0.5399085526556401 1.8926230163368418 7 0.3546344020295672 1.4503458034860728 8 0.25573994140135403 1.1953118033590426 9 0.1908212479686816 1.003371823252059 >10 1.1142710293326537 12.224628259718848 >50 0.09435593234359455 3.8292525214678017 >100 0.04364144116575537 4.6054091930237 >500 0.001014909037566787 0.40365229828660704 >1k 3.8059088908754505E-4 0.45479443670338243 >5k 2.5372725939169674E-4 0.832505070575683 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6011 0.44258538783468176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5221 0.38441828479202683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3524 0.2594694571168555 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1562 0.11500887968686954 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.4725848871558202E-4 0.0 0.0 0.0 0.0 7 2.2088773307337302E-4 0.0 0.0 0.0 0.0 8 2.2088773307337302E-4 0.0 0.0 0.0 0.0 9 2.2088773307337302E-4 0.0 0.0 0.0 0.0 10-11 2.2088773307337302E-4 0.0 0.0 0.0 0.0 12-13 2.2088773307337302E-4 0.0 0.0 3.6814622178895505E-5 0.0 14-15 2.2088773307337302E-4 0.0 0.0 1.4725848871558202E-4 3.6814622178895505E-5 16-17 2.5770235525226853E-4 0.0 0.0 3.3133159961005955E-4 7.362924435779101E-5 18-19 2.9451697743116404E-4 0.0 0.0 4.049608439678505E-4 7.362924435779101E-5 20-21 2.9451697743116404E-4 0.0 0.0 6.258485770412235E-4 7.362924435779101E-5 22-23 2.9451697743116404E-4 0.0 0.0 9.571801766512831E-4 7.362924435779101E-5 24-25 3.68146221788955E-4 0.0 0.0 0.0012885117762613425 7.362924435779101E-5 26-27 3.68146221788955E-4 0.0 0.0 0.0016566579980502975 7.362924435779101E-5 28-29 3.68146221788955E-4 0.0 0.0 0.0022825065750915214 7.362924435779101E-5 30-31 3.68146221788955E-4 0.0 0.0 0.00474908626107752 7.362924435779101E-5 32-33 3.68146221788955E-4 0.0 0.0 0.01023446496573295 7.362924435779101E-5 34-35 4.049608439678505E-4 0.0 0.0 0.015241253582062738 7.362924435779101E-5 36-37 4.4177546614674603E-4 0.0 0.0 0.02415039214935545 7.362924435779101E-5 38-39 4.4177546614674603E-4 0.0 0.0 0.04303629332712884 7.362924435779101E-5 40-41 4.4177546614674603E-4 0.0 0.0 0.06604543218893853 7.362924435779101E-5 42-43 4.7859008832564154E-4 0.0 0.0 0.08728746918616123 7.362924435779101E-5 44-45 5.154047105045371E-4 0.0 0.0 0.11556109901955297 7.362924435779101E-5 46-47 5.522193326834325E-4 0.0 0.0 0.14814203964787548 7.362924435779101E-5 48-49 6.62663199220119E-4 0.0 0.0 0.1834840769396152 7.362924435779101E-5 50-51 6.62663199220119E-4 0.0 0.0 0.22405379058075803 7.362924435779101E-5 52-53 6.62663199220119E-4 0.0 0.0 0.2656911282650889 7.362924435779101E-5 54-55 6.62663199220119E-4 0.0 0.0 0.3113412597669193 7.362924435779101E-5 56-57 6.62663199220119E-4 0.0 0.0 0.3659373444582213 7.362924435779101E-5 58-59 6.62663199220119E-4 0.0 0.0 0.4269391734086511 7.362924435779101E-5 60-61 6.62663199220119E-4 0.0 0.0 0.49972168145632756 7.362924435779101E-5 62-63 6.62663199220119E-4 0.0 0.0 0.5748603253234532 7.362924435779101E-5 64-65 6.62663199220119E-4 0.0 0.0 0.647532389504593 7.362924435779101E-5 66-67 6.62663199220119E-4 0.0 0.0 0.7290767776308466 7.362924435779101E-5 68-69 6.62663199220119E-4 0.0 0.0 0.8256415316060894 7.362924435779101E-5 70-71 7.3629244357791E-4 0.0 0.0 0.9441109857777752 7.362924435779101E-5 72-73 8.09921687935701E-4 0.0 0.0 1.0632062885265021 7.362924435779101E-5 74-75 8.09921687935701E-4 0.0 0.0 1.1842159516285315 7.362924435779101E-5 76-77 8.09921687935701E-4 0.0 0.0 1.3149446749857896 7.362924435779101E-5 78-79 8.09921687935701E-4 0.0 0.0 1.473321179599398 7.362924435779101E-5 80-81 9.571801766512831E-4 0.0 0.0 1.6541546037421329 7.362924435779101E-5 82-83 9.571801766512831E-4 0.0 0.0 1.8462901168937884 7.362924435779101E-5 84-85 9.571801766512831E-4 0.0 0.0 2.037063489024825 7.362924435779101E-5 86-87 9.939947988301787E-4 0.0 0.0 2.230045738486595 7.362924435779101E-5 88-89 0.0010308094210090741 0.0 0.0 2.4630086676346457 7.362924435779101E-5 90-91 0.0010308094210090741 0.0 0.0 2.7248342605709506 7.362924435779101E-5 92-93 0.0010308094210090741 0.0 0.0 3.0138290446752802 7.362924435779101E-5 94-95 0.0010308094210090741 0.0 0.0 3.2967494161200923 7.362924435779101E-5 96-97 0.0010308094210090741 0.0 0.0 3.574405296593322 7.362924435779101E-5 98-99 0.0010308094210090741 0.0 0.0 3.8875136582248286 7.362924435779101E-5 100-101 0.0010308094210090741 0.0 0.0 4.261034814851902 7.362924435779101E-5 102-103 0.0010308094210090741 0.0 0.0 4.666474248908078 7.362924435779101E-5 104-105 0.001104438665366865 0.0 0.0 5.061495144887627 1.1044386653668651E-4 106-107 0.001251697154082447 0.0 0.0 5.445213951858255 1.4725848871558202E-4 108-109 0.0013989556427980291 0.0 0.0 5.860114743814407 1.4725848871558202E-4 110-111 0.0013989556427980291 0.0 0.0 6.33598055009881 1.4725848871558202E-4 112-113 0.0013989556427980291 0.0 0.0 6.865522075520044 1.4725848871558202E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4620 0.0 49.174103 1 GTATCAA 7555 0.0 44.987705 1 ATCAACG 9820 0.0 34.336655 3 TCAACGC 9855 0.0 33.85201 4 TATCAAC 10020 0.0 33.472927 2 CAACGCA 9905 0.0 33.45875 5 GTACATG 8515 0.0 32.77288 1 AACGCAG 10200 0.0 32.374405 6 TACATGG 8560 0.0 31.45703 2 ACATGGG 8680 0.0 29.992641 3 ACGCAGA 11760 0.0 27.77628 7 GAGTACT 6730 0.0 27.53823 12-13 CGCAGAG 11850 0.0 27.414688 8 TACCTGG 1160 0.0 27.218922 2 GTGTACG 155 6.718992E-6 26.928974 1 CATGGGG 3105 0.0 26.668966 4 AGTACTT 7025 0.0 26.127743 12-13 TATAACG 160 8.6594955E-6 26.0634 2 GCAGAGT 12365 0.0 25.262379 9 GTACTTT 7515 0.0 24.661648 14-15 >>END_MODULE