FastQCFastQC Report
Thu 26 May 2016
SRR1364582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104496
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.12582585094008258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44250.053.11411
GGTATCA23150.052.4330521
ATCAACG52900.043.529263
CAACGCA53550.043.3342325
TCAACGC53200.043.2837944
AACGCAG55200.041.814396
TATCAAC58850.039.4315762
GTACATG75900.035.5125961
ACGCAGA65650.034.977257
CGCAGAG65900.033.944278
TACATGG76000.033.821762
ACATGGG78900.032.4277953
GCAGAGT74000.027.7378039
GTACCGT2351.043918E-825.314266
CATGGGG45150.023.9849954
GAGTACA54200.023.6028881
CATGGGA43800.023.3657824
AGAGTAC67300.023.34324810-11
AGTACAT52350.022.5047652
CAGAGTA73500.021.94089910-11
Produced by FastQC (version 0.11.5)