##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364582_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104496 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.953197820285713 31.0 27.0 31.0 7.0 34.0 2 27.020726102591784 31.0 27.0 33.0 10.0 34.0 3 27.15435097049365 31.0 27.0 33.0 10.0 34.0 4 30.405372117599654 35.0 30.0 37.0 10.0 37.0 5 30.4084251051083 35.0 30.0 37.0 7.0 37.0 6 30.47623991682569 35.0 31.0 37.0 8.0 37.0 7 30.23112659753214 35.0 30.0 37.0 2.0 37.0 8 30.177831651853936 35.0 30.0 37.0 2.0 37.0 9 31.146396571910806 37.0 30.0 39.0 2.0 39.0 10-11 31.06041090123241 37.0 30.0 39.0 2.0 39.0 12-13 30.939990620081957 37.0 30.0 39.0 2.0 39.0 14-15 31.543134080558957 38.0 30.0 40.0 2.0 41.0 16-17 31.60940814332743 38.0 30.0 40.0 2.0 41.0 18-19 31.415130748644806 38.0 29.5 40.0 2.0 41.0 20-21 31.13166881761714 37.5 28.5 40.0 2.0 41.0 22-23 30.991890932555826 37.0 27.5 40.0 2.0 41.0 24-25 30.816519727288625 37.0 27.0 40.0 2.0 41.0 26-27 30.669267606115667 37.0 27.0 40.0 2.0 41.0 28-29 30.69701724308338 37.0 27.5 40.0 2.0 41.0 30-31 30.633576162653668 37.0 27.5 40.0 2.0 41.0 32-33 30.58546963263413 37.0 28.0 40.0 2.0 41.0 34-35 30.178139088883988 36.0 27.0 40.0 2.0 41.0 36-37 30.02196274072272 36.0 26.5 40.0 2.0 41.0 38-39 29.823458443256726 36.0 25.5 40.0 2.0 41.0 40-41 29.986890923052357 36.0 27.0 40.0 2.0 41.0 42-43 29.868846745253972 36.0 26.5 40.0 2.0 41.0 44-45 29.716018467129423 36.0 26.5 40.0 2.0 41.0 46-47 29.672020521778137 36.0 27.0 40.0 2.0 41.0 48-49 29.52103686583391 36.0 26.0 40.0 2.0 41.0 50-51 29.357374402232175 36.0 26.0 40.0 2.0 41.0 52-53 29.29588438276908 35.5 26.0 40.0 2.0 41.0 54-55 29.316891787867497 36.0 26.0 40.0 2.0 41.0 56-57 29.1895444324912 35.5 26.0 40.0 2.0 41.0 58-59 28.986191943344153 35.0 26.0 39.5 2.0 41.0 60-61 28.782458365328324 35.0 26.0 39.0 2.0 41.0 62-63 28.49906105784948 35.0 25.0 39.0 2.0 41.0 64-65 28.231085732640974 34.0 24.0 39.0 2.0 40.5 66-67 27.98739056762284 34.0 23.5 38.5 2.0 40.0 68-69 27.945590535691203 34.0 24.5 38.0 2.0 40.0 70-71 27.7348754761235 34.0 24.0 37.5 2.0 40.0 72-73 27.458395264234287 34.0 24.0 37.0 2.0 39.5 74-75 27.170302533243113 34.0 23.0 36.5 2.0 39.0 76-77 26.879194353422385 34.0 22.5 36.0 2.0 39.0 78-79 26.585995411728035 34.0 22.0 36.0 2.0 38.5 80-81 26.302504732724604 33.0 21.5 35.0 2.0 37.0 82-83 26.06524103633364 33.0 20.5 35.0 2.0 37.0 84-85 25.82550501402711 33.0 20.0 35.0 2.0 36.0 86-87 25.635579492667127 33.0 19.5 35.0 2.0 36.0 88-89 25.447180702648048 33.0 18.5 35.0 2.0 36.0 90-91 25.309382151355955 33.0 18.0 35.0 2.0 35.5 92-93 25.183663927135047 33.0 18.0 35.0 2.0 35.0 94-95 25.062856142278246 33.0 17.0 35.0 2.0 35.0 96-97 24.949945735225917 33.0 14.0 35.0 2.0 35.0 98-99 24.861940816233435 33.0 11.0 35.0 2.0 35.0 100-101 24.7676329629517 33.0 9.0 35.0 2.0 35.0 102-103 23.695225602709627 30.5 7.0 34.0 2.0 35.0 104-105 24.50865813002258 32.0 7.0 35.0 2.0 35.0 106-107 24.650850607461358 33.0 7.0 35.0 2.0 35.0 108-109 24.6107923227224 33.0 2.0 35.0 2.0 35.0 110-111 24.52458664687412 33.0 2.0 35.0 2.0 35.0 112-113 24.439666314404967 33.0 2.0 35.0 2.0 35.0 114-115 24.319825031741566 33.0 2.0 35.0 2.0 35.0 116-117 24.209129169169245 33.0 2.0 35.0 2.0 35.0 118-119 24.062833571553476 32.5 2.0 35.0 2.0 35.0 120-121 23.882951547543925 32.0 2.0 35.0 2.0 35.0 122-123 23.642736788285653 32.0 2.0 35.0 2.0 35.0 124-125 22.276205799393296 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8305523041709115 1101 2 0.7855802942944017 1101 3 0.7383839115580493 1101 4 0.8584777757917976 1101 5 0.8553051660852269 1101 6 0.8131223255966731 1101 7 0.9213692467951162 1101 8 0.8413036241970069 1101 9 1.1527510706837205 1101 10-11 1.1660016312996575 1101 12-13 0.9135170514173367 1101 14-15 1.2099570892831615 1101 16-17 0.8299466824269324 1101 18-19 1.3447747941950752 1101 20-21 1.5931913311709778 1101 22-23 1.549463216764412 1101 24-25 1.6923643848572034 1101 26-27 1.9166626924167467 1101 28-29 1.593541204144337 1101 30-31 1.6957197853884765 1101 32-33 1.6471853922871915 1101 34-35 1.878419103148243 1101 36-37 1.8114031829686255 1101 38-39 1.6282981097318476 1101 40-41 1.5058495279842 1101 42-43 1.5731431306394086 1101 44-45 1.3686319133764684 1101 46-47 1.2291926387635002 1101 48-49 1.299771614728357 1101 50-51 1.3707385681413662 1101 52-53 1.1464951833836174 1101 54-55 1.0806611037441947 1101 56-57 1.0654265048771485 1101 58-59 1.1200052808398553 1101 60-61 1.0576860769761147 1101 62-63 1.0791871572132123 1101 64-65 1.0390198074424895 1101 66-67 1.0297655248680968 1101 68-69 0.8749771721286308 1101 70-71 0.8621731313285785 1101 72-73 0.804721209128779 1101 74-75 0.7021561897345485 1101 76-77 0.8858626848730751 1101 78-79 0.9823752696697063 1101 80-81 1.0389636767208117 1101 82-83 0.8721274453212509 1101 84-85 1.055416393112555 1101 86-87 1.007278396720995 1101 88-89 0.9643768015862797 1101 90-91 0.9274716956157114 1101 92-93 0.8269535255084755 1101 94-95 0.9645506619888025 1101 96-97 0.8856472076223056 1101 98-99 0.954572345027767 1101 100-101 0.9787339787303253 1101 102-103 0.9526786359006962 1101 104-105 1.0216725693497217 1101 106-107 0.9806164549621279 1101 108-109 1.0553857577661496 1101 110-111 0.8783191415923852 1101 112-113 0.8642927538194591 1101 114-115 1.1144218215137478 1101 116-117 1.139095782091907 1101 118-119 1.0292243794683493 1101 120-121 1.0902067234275385 1101 122-123 1.1910198417313715 1101 124-125 1.3995988118744371 1102 1 -0.4242341082467078 1102 2 -0.08956271892596135 1102 3 0.061277124118849 1102 4 0.17787795349810054 1102 5 0.12801521710292718 1102 6 -0.5017948406977162 1102 7 -1.2750883222753195 1102 8 -0.38009616043311567 1102 9 -0.04240527424585849 1102 10-11 -0.05189166719985394 1102 12-13 0.12671333243840976 1102 14-15 0.25813647774331017 1102 16-17 0.1285594074835963 1102 18-19 0.3201019778042493 1102 20-21 0.48199177999522647 1102 22-23 0.46636085927881155 1102 24-25 0.5858637193926803 1102 26-27 0.06634961396516914 1102 28-29 0.6536122679833412 1102 30-31 0.4283006630543582 1102 32-33 0.24751668600850962 1102 34-35 0.700950065370332 1102 36-37 0.8030152847828269 1102 38-39 0.7341610539322154 1102 40-41 0.503886669199435 1102 42-43 0.4661111397683406 1102 44-45 0.51217842078999 1102 46-47 0.09312597498636777 1102 48-49 -0.22051216236008386 1102 50-51 0.12519607817564804 1102 52-53 -0.10040476453005098 1102 54-55 -0.19926789220024688 1102 56-57 -0.1443271835748554 1102 58-59 0.07994569915959815 1102 60-61 -0.05113979102352317 1102 62-63 -0.09438512060346227 1102 64-65 -0.1909918764854055 1102 66-67 -0.1050791278386356 1102 68-69 -0.16666041238087814 1102 70-71 -0.1807035585813921 1102 72-73 -0.0034833033259786816 1102 74-75 -0.28670279082312433 1102 76-77 -0.04704795456322586 1102 78-79 -0.11015334978846525 1102 80-81 -0.5218639279880293 1102 82-83 -0.34510235030155556 1102 84-85 -0.6827201418237756 1102 86-87 -0.20969694559530083 1102 88-89 -0.013204218065290263 1102 90-91 -0.5178370036981974 1102 92-93 -0.21653407652506118 1102 94-95 -0.18705409393405859 1102 96-97 -0.035094230453623254 1102 98-99 0.035121575577409914 1102 100-101 0.12967420424844533 1102 102-103 -0.046996571828110234 1102 104-105 0.2169302659083634 1102 106-107 0.15810296071665775 1102 108-109 -0.3645601867861892 1102 110-111 -0.14833418838311374 1102 112-113 -0.4400605835542386 1102 114-115 -0.3436399019085883 1102 116-117 -0.4212695284121182 1102 118-119 -0.16753051885121195 1102 120-121 0.2668069395447077 1102 122-123 0.0728250665059349 1102 124-125 0.25822898248892656 1103 1 0.3911831661953009 1103 2 0.3025360379263837 1103 3 0.2370519857500284 1103 4 0.3141297919319612 1103 5 0.21734856151533677 1103 6 0.42760967669403627 1103 7 0.48132419546693583 1103 8 0.6112055073955958 1103 9 0.7341129303096032 1103 10-11 0.8418096525444909 1103 12-13 0.6781540716040197 1103 14-15 0.9565386171628809 1103 16-17 0.8087797476061347 1103 18-19 0.8056919422781874 1103 20-21 1.2188471195172284 1103 22-23 1.0345351235464548 1103 24-25 1.155880572175203 1103 26-27 1.1001033352121397 1103 28-29 1.0803514811827526 1103 30-31 1.0639420507990387 1103 32-33 0.9564973268372619 1103 34-35 1.4010795016897006 1103 36-37 1.337490121561629 1103 38-39 1.304608763094432 1103 40-41 1.0273120180203072 1103 42-43 1.0069111521567606 1103 44-45 0.6383339808107493 1103 46-47 0.6301274072084944 1103 48-49 0.6607520153925783 1103 50-51 0.6663765245666546 1103 52-53 0.7030642005912604 1103 54-55 0.579967172011802 1103 56-57 0.5079150636541776 1103 58-59 0.4701087655048717 1103 60-61 0.3653998439864523 1103 62-63 0.4381443000037635 1103 64-65 0.6179623634133122 1103 66-67 0.6104754956478438 1103 68-69 0.679209806085872 1103 70-71 0.6539979145074106 1103 72-73 0.7585477213549225 1103 74-75 0.6735636206219624 1103 76-77 0.7472072181683558 1103 78-79 0.7363853900826811 1103 80-81 0.7504237069343205 1103 82-83 0.7284347209099487 1103 84-85 0.6467549668959371 1103 86-87 0.5512670887085314 1103 88-89 0.48416286514938633 1103 90-91 0.6536781113063661 1103 92-93 0.6697927345069807 1103 94-95 0.591070167198712 1103 96-97 0.6762275572254879 1103 98-99 0.5812350111957763 1103 100-101 0.5028886477855821 1103 102-103 0.4822112982929134 1103 104-105 0.5163308817034462 1103 106-107 0.6079596063104233 1103 108-109 0.8139855449879114 1103 110-111 0.8113415069735694 1103 112-113 0.7696031199636941 1103 114-115 0.8635792605587334 1103 116-117 0.8741154266080997 1103 118-119 0.9085331478036167 1103 120-121 0.7777090317523161 1103 122-123 0.7762484815247745 1103 124-125 0.8244744491961846 1104 1 0.35374722959473814 1104 2 0.4909314132635849 1104 3 0.40833381046264705 1104 4 0.5705668230660343 1104 5 0.47047237156256116 1104 6 0.369095851204861 1104 7 0.4347499568671971 1104 8 0.5929845757894867 1104 9 0.706951940383096 1104 10-11 0.6807010653278027 1104 12-13 0.651151355433651 1104 14-15 0.7173971425510963 1104 16-17 0.8234843966434475 1104 18-19 1.0307009265889207 1104 20-21 1.2490493923069579 1104 22-23 0.8732748647483426 1104 24-25 1.4274726277248355 1104 26-27 1.3560831211375195 1104 28-29 1.3234287441138264 1104 30-31 1.145534134898611 1104 32-33 1.1110549507966638 1104 34-35 1.405847431679529 1104 36-37 1.3922948631364491 1104 38-39 1.3945558993030076 1104 40-41 1.139133675794259 1104 42-43 1.2646761125954598 1104 44-45 1.1482110126300178 1104 46-47 1.006818233076313 1104 48-49 0.9291199792140894 1104 50-51 0.6852206479352461 1104 52-53 0.8905777395103698 1104 54-55 0.77111163937154 1104 56-57 0.7250034011088147 1104 58-59 0.6847100458985196 1104 60-61 0.6259729591458658 1104 62-63 0.7569576072757584 1104 64-65 0.7153028646861088 1104 66-67 0.6294502350136142 1104 68-69 0.7286904290599416 1104 70-71 0.7410584728658165 1104 72-73 0.5935822150412804 1104 74-75 0.7347893339869422 1104 76-77 0.6402245975903398 1104 78-79 0.6943957372964036 1104 80-81 0.6645027374638488 1104 82-83 0.7050948734308911 1104 84-85 0.729897491431938 1104 86-87 0.7174469490778925 1104 88-89 0.7631676461945069 1104 90-91 0.8040003509638822 1104 92-93 0.7595212852011528 1104 94-95 0.7601381221340517 1104 96-97 0.6007979786976136 1104 98-99 0.6806774129313276 1104 100-101 0.7692738179450345 1104 102-103 0.6952384061085652 1104 104-105 0.7164950795731215 1104 106-107 0.7073952274340982 1104 108-109 0.7501669613292599 1104 110-111 0.7507092755848355 1104 112-113 0.677133953011829 1104 114-115 0.6908624442487223 1104 116-117 0.7712850386179184 1104 118-119 0.7939199299643285 1104 120-121 0.6820683228143629 1104 122-123 0.7339290768767697 1104 124-125 1.0680356336905668 1105 1 0.046497856915976854 1105 2 0.5062939750442794 1105 3 0.6122056284584936 1105 4 0.614725838243988 1105 5 0.4547185046857791 1105 6 0.5100407354695697 1105 7 0.5930152590610085 1105 8 0.36384709880339017 1105 9 0.29503905548959963 1105 10-11 0.5024534769860836 1105 12-13 0.5021183607227897 1105 14-15 0.6353116505046792 1105 16-17 0.5600268191369189 1105 18-19 0.7425252599641965 1105 20-21 1.0992672315257153 1105 22-23 1.312694874481732 1105 24-25 1.3447731211187808 1105 26-27 1.1052051020514817 1105 28-29 0.8047356534564791 1105 30-31 0.7746173561157761 1105 32-33 0.7633439126219272 1105 34-35 1.3279620156763485 1105 36-37 1.44514336197442 1105 38-39 1.544340943198197 1105 40-41 1.1055874030573598 1105 42-43 0.6777909011603 1105 44-45 0.9759987576037759 1105 46-47 0.8651165979337634 1105 48-49 0.7392608490486801 1105 50-51 0.7239624694280877 1105 52-53 0.7703617791947899 1105 54-55 0.4894484835984052 1105 56-57 0.6641336357126768 1105 58-59 0.6284545491308862 1105 60-61 0.629926817257978 1105 62-63 0.656773256534386 1105 64-65 0.6588708326336885 1105 66-67 0.43237298512463695 1105 68-69 0.33751112545349216 1105 70-71 0.5020057895915855 1105 72-73 0.5647015747167572 1105 74-75 0.5340034680937897 1105 76-77 0.6392263748304856 1105 78-79 0.6103764298363394 1105 80-81 0.5442639340146869 1105 82-83 0.46370096856442444 1105 84-85 0.6317803777465834 1105 86-87 0.4967959845298999 1105 88-89 0.6129093280559204 1105 90-91 0.4324958161361323 1105 92-93 0.42362702220757953 1105 94-95 0.4469416837822138 1105 96-97 0.5261343759974579 1105 98-99 0.356212301537564 1105 100-101 0.37020950818114073 1105 102-103 0.41158421619882546 1105 104-105 0.49986716787199015 1105 106-107 0.5042419257895894 1105 108-109 0.5333627804769314 1105 110-111 0.5854136996300383 1105 112-113 0.5545717589213055 1105 114-115 0.5422787274020919 1105 116-117 0.5736379967016987 1105 118-119 0.5753107423584183 1105 120-121 0.43157162881459143 1105 122-123 0.6054225671603248 1105 124-125 0.5451634028328023 1106 1 0.1906659949690983 1106 2 0.4708400145662246 1106 3 0.2266835219639809 1106 4 0.5021363536914869 1106 5 0.1625963489467317 1106 6 0.11397837614372719 1106 7 -0.12077321094421833 1106 8 0.29086221175003146 1106 9 0.4474588569187894 1106 10-11 0.6247088492628734 1106 12-13 0.5833856203376051 1106 14-15 0.8282093619152242 1106 16-17 0.6602740944404211 1106 18-19 0.6981444459282145 1106 20-21 1.0314151719965778 1106 22-23 1.0796703263552665 1106 24-25 1.1643357417701772 1106 26-27 1.3765021738322538 1106 28-29 1.1414699260972938 1106 30-31 0.9942809829849821 1106 32-33 0.8889201331861898 1106 34-35 1.255792958635979 1106 36-37 1.1503613148113345 1106 38-39 1.3575195772939033 1106 40-41 1.0192734023232681 1106 42-43 0.9303977036796454 1106 44-45 0.6206504623525788 1106 46-47 0.4429852523222486 1106 48-49 0.6943992600611502 1106 50-51 0.5614590712805239 1106 52-53 0.5216664390276335 1106 54-55 0.4012450212864884 1106 56-57 0.44187345797468325 1106 58-59 0.32008897344121223 1106 60-61 0.4965393881614162 1106 62-63 0.5299864716442215 1106 64-65 0.4768532345771064 1106 66-67 0.1919676147242626 1106 68-69 0.2524674140630836 1106 70-71 0.43939781545503465 1106 72-73 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-1.5435898590378834 2216 104-105 -1.7317394053023918 2216 106-107 -1.652815622337524 2216 108-109 -1.673763025291752 2216 110-111 -1.712063322730728 2216 112-113 -1.6354764959461576 2216 114-115 -1.805749101274877 2216 116-117 -1.7801242038954612 2216 118-119 -1.851365950029578 2216 120-121 -1.84516237079119 2216 122-123 -1.7845453845994115 2216 124-125 -1.8662418285496543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 206360.0 3 31623.0 4 19075.0 5 14477.0 6 13257.0 7 14114.0 8 15023.0 9 15107.0 10 15697.0 11 16298.0 12 17287.0 13 18412.0 14 19189.0 15 17130.0 16 13920.0 17 12590.0 18 11655.0 19 10878.0 20 10749.0 21 11526.0 22 13914.0 23 18144.0 24 24375.0 25 30579.0 26 36821.0 27 41787.0 28 47768.0 29 54682.0 30 62248.0 31 70490.0 32 84902.0 33 105198.0 34 133317.0 35 179163.0 36 266592.0 37 327208.0 38 102584.0 39 357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82164743319531 18.35150228278169 12.900104598782166 23.92674568524083 2 15.124309359447668 19.467949573913923 41.12024071618006 24.28750035045835 3 19.70077645293262 24.687819730239752 28.035420586196064 27.57598323063156 4 12.107033456033653 15.971238132712134 37.28264888224856 34.63907952900565 5 13.46288560334954 38.17951971561233 34.16021519173831 14.197379489299818 6 32.50437044453325 36.31092190601276 17.528963253102788 13.655744396351208 7 28.98557680655832 31.92153851198416 20.712924228693463 18.379960452764053 8 25.70734275570018 36.67671499494416 19.209444921729478 18.406497327626187 9 26.1501667431718 16.64371512720285 19.360692708823372 37.84542542080198 10-11 24.888666930229377 26.9675494750287 27.324974720788255 20.818808873953667 12-13 25.65529963377065 24.06124920940174 27.647607249316795 22.635843907510814 14-15 22.52949304868239 25.666491215354803 25.300704934063177 26.503310801899627 16-17 22.4864987418472 28.931264075198275 25.802161532180097 22.780075650774428 18-19 22.31997390190929 27.745116884233916 27.806156573813002 22.128752640043796 20-21 22.998194417020525 26.896081915823572 28.07960722451791 22.026116442637992 22-23 22.661847684591095 26.853882156215704 27.831545974718875 22.65272418447432 24-25 22.494387565011102 26.851818409927308 28.082609720490282 22.571184304571307 26-27 22.444374422856566 27.059264402624766 27.82433738111974 22.672023793398928 28-29 22.343616542309093 27.138999730337503 27.991513353045228 22.525870374308173 30-31 22.511328343286817 26.988162261411023 27.757981159976737 22.742528235325423 32-33 22.416344926283454 27.01459694279908 27.775131094009176 22.79392703690829 34-35 22.63845281277607 26.86893892484421 27.993179708873555 22.499428553506164 36-37 22.617628448148245 26.7777775929678 27.833253772638265 22.771340186245688 38-39 22.456575092837 26.969320430792187 27.96818239054975 22.605922085821064 40-41 22.751483318650852 26.990398917480974 27.592281586126116 22.665836177742058 42-43 22.59182274001936 26.91837044070258 27.524748146532406 22.96505867274565 44-45 22.68722733897093 27.0937170962193 27.470069218089137 22.748986346720628 46-47 22.757905441205473 27.28621629391681 27.053133783658563 22.902744481219155 48-49 22.809109641892395 27.222390025050018 27.021961363418708 22.946538969638883 50-51 22.82351599448298 27.38893198647119 27.0028119687464 22.784740050299433 52-53 22.902158046392103 27.443197801278785 26.814710030335053 22.839934121994055 54-55 22.8054471324091 27.2193609495127 26.94347796952028 23.031713948557922 56-57 22.71027040358222 27.505504560344402 26.930231375673202 22.853993660400167 58-59 22.870220338419283 27.20787459194389 26.923197544298144 22.99870752533868 60-61 22.914205555782594 27.273472463911364 27.029175034047675 22.783146946258366 62-63 22.795740985693175 27.25530558788875 27.121795508566937 22.82715791785114 64-65 22.68790567201206 27.146979952688678 27.01112921028588 23.153985165013385 66-67 22.73524068747621 27.38082283692992 26.863514522314848 23.020421953279026 68-69 22.639856753985427 27.323298032743644 27.08440417333528 22.952441039935646 70-71 22.663801597440823 27.08687893173847 27.128838066962125 23.120481403858577 72-73 22.709502443503123 27.30471875180438 26.984177524238405 23.00160128045409 74-75 22.67408570702235 27.404507969845497 26.83748357682009 23.083922746312062 76-77 22.80509838173615 27.232609152262405 26.921611097752564 23.040681368248876 78-79 22.728467153978364 27.312113115362486 26.89742457843325 23.061995152225904 80-81 22.683698783011824 27.340286487964715 26.893488052388868 23.082526676634593 82-83 22.83444680372343 27.414916868084465 26.83726178231767 22.913374545874436 84-85 22.85376166074918 27.30995924915039 26.73576001094799 23.10051907915244 86-87 22.628536915456174 27.252282861998044 27.076910209962477 23.0422700125833 88-89 22.893543711237317 27.206306584606608 26.85723451274862 23.042915191407456 90-91 22.88218846822338 27.335867497256476 26.887160790609716 22.894783243910428 92-93 22.74940285588494 27.288981161537667 26.934080469342327 23.027535513235062 94-95 22.70317683211276 27.271027046834874 26.87418741030471 23.151608710747652 96-97 22.7608139137824 27.202270582680395 26.924911228134963 23.11200427540225 98-99 22.65092020662655 27.217479150362113 26.79784915273325 23.333751490278086 100-101 22.6158515302208 27.192483274292105 26.98524297985977 23.206422215627327 102-103 22.57186518767439 26.988647163026208 27.09660650340984 23.342881145889564 104-105 22.623316318724086 27.019894833178583 27.104690691130195 23.252098156967136 106-107 22.524073471307844 27.063285966467017 27.06528169465834 23.347358867566804 108-109 22.505459739529083 27.083658034987952 26.926375721312844 23.48450650417012 110-111 22.43697602777317 27.132822074083013 27.0425621187592 23.387639779384617 112-113 22.482889014756978 27.15191665843033 27.083396689753748 23.281797637058943 114-115 22.529669811679376 27.218227071945016 26.923429647763648 23.328673468611964 116-117 22.286191088032478 27.104874516273732 27.13972846705339 23.469205928640395 118-119 22.422328196394766 27.104114239228778 26.991569477917754 23.481988086458706 120-121 22.417563358430407 27.218216246653 27.050955217284905 23.31326517763169 122-123 22.42681858269153 27.15205921037626 27.008960815321103 23.412161391611104 124-125 22.49885397979725 27.148024460788285 26.8193204391307 23.533801120283762 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 591.0 1 680.0 2 808.0 3 736.5 4 550.5 5 464.5 6 465.0 7 571.5 8 761.0 9 879.5 10 862.5 11 872.5 12 977.0 13 1099.0 14 1191.0 15 1296.5 16 1439.5 17 1561.5 18 1818.5 19 2063.0 20 2391.0 21 2975.5 22 3678.0 23 4715.0 24 6002.5 25 7737.5 26 10253.5 27 13326.5 28 16146.0 29 19217.0 30 23261.5 31 27342.0 32 31470.0 33 35860.0 34 40561.0 35 44828.0 36 49662.0 37 54517.0 38 57397.5 39 60102.5 40 62489.5 41 64420.0 42 66288.0 43 69151.0 44 72673.5 45 74977.5 46 76145.5 47 76641.5 48 77519.5 49 78186.0 50 77234.5 51 75578.5 52 74803.5 53 74081.0 54 72520.0 55 68590.0 56 62576.5 57 57015.5 58 52896.0 59 48372.5 60 41854.5 61 34383.0 62 27517.5 63 21704.5 64 16423.5 65 12164.5 66 9232.5 67 7276.5 68 5585.0 69 4155.0 70 3107.0 71 2228.5 72 1603.0 73 1260.0 74 933.0 75 626.0 76 415.5 77 262.0 78 183.5 79 128.0 80 99.0 81 78.5 82 58.0 83 40.0 84 34.0 85 29.5 86 18.5 87 14.5 88 9.5 89 7.5 90 8.0 91 7.5 92 8.5 93 4.5 94 3.0 95 6.5 96 5.5 97 2.5 98 3.5 99 4.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012592088557070197 2 0.005179387368757175 3 0.00869566870167489 4 0.008648151386365191 5 0.015490644790961825 6 9.028289908842782E-4 7 0.008410564809816697 8 0.0 9 0.002946073549201329 10-11 0.0 12-13 0.0036588332788468118 14-15 0.018935650150914994 16-17 0.029365700861393895 18-19 0.006058457701986604 20-21 0.009004531251187933 22-23 0.0019244512700428036 24-25 0.011404155674327725 26-27 0.01819913176361466 28-29 6.414837566809345E-4 30-31 0.0019006926123879542 32-33 0.008814461989949137 34-35 0.008885737962913686 36-37 0.011047775809504983 38-39 4.7517315309698855E-5 40-41 0.02444765872684006 42-43 0.009360911116010675 44-45 0.01660730170073975 46-47 0.004324075693182596 48-49 0.02402000288905277 50-51 0.004680455558005337 52-53 0.0 54-55 0.016298439151226706 56-57 0.004965559449863531 58-59 2.8510389185819313E-4 60-61 0.004466627639111692 62-63 0.025421763690688888 64-65 0.01655978438543005 66-67 0.002138279188936449 68-69 0.020123583033657463 70-71 0.003207418783404673 72-73 0.011522948962601972 74-75 4.7517315309698855E-5 76-77 0.006747458773977238 78-79 0.0011879328827424714 80-81 0.003777626567121059 82-83 0.0018769339547331048 84-85 0.0 86-87 0.005702077837163862 88-89 0.016440991097155803 90-91 0.021335274574054784 92-93 0.004965559449863531 94-95 0.02363986436657518 96-97 0.01741509606100463 98-99 0.0010928982521230736 100-101 0.008339288836852149 102-103 0.0 104-105 0.0014255194592909656 106-107 4.7517315309698855E-5 108-109 0.0 110-111 9.028289908842782E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.7517315309698855E-5 122-123 0.0 124-125 0.022594483429761805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2104496.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.71263759579534 #Duplication Level Percentage of deduplicated Percentage of total 1 83.82857722737859 57.60082647196006 2 9.562347766135478 13.141082732388604 3 2.9339883282675934 6.048062301316337 4 1.2626513996356095 3.4704043213194162 5 0.6699347963276895 2.3016493436438745 6 0.3942548234557239 1.6254173282704454 7 0.25957641507345 1.24853260961504 8 0.17458494146023765 0.9596953449792364 9 0.13598149770931361 0.8409282634650187 >10 0.7215336431077668 8.838192444123402 >50 0.040663409096176675 1.878628449247208 >100 0.015415864465099016 1.6631760316121638 >500 2.7993593553733226E-4 0.10762058865380589 >1k 2.099519516528882E-4 0.27578376940539523 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.12582585094008258 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.7517315309698855E-5 5 0.0 0.0 0.0 0.0 4.7517315309698855E-5 6 0.0 0.0 0.0 0.0 4.7517315309698855E-5 7 0.0 0.0 0.0 0.0 4.7517315309698855E-5 8 0.0 0.0 0.0 0.0 4.7517315309698855E-5 9 0.0 0.0 0.0 0.0 4.7517315309698855E-5 10-11 0.0 0.0 0.0 7.127597296454828E-5 9.503463061939771E-5 12-13 0.0 0.0 0.0 9.503463061939771E-5 9.503463061939771E-5 14-15 0.0 0.0 0.0 9.503463061939771E-5 9.503463061939771E-5 16-17 0.0 0.0 0.0 1.4255194592909656E-4 9.503463061939771E-5 18-19 0.0 0.0 0.0 1.4255194592909656E-4 1.1879328827424714E-4 20-21 0.0 0.0 0.0 2.1382791889364486E-4 1.4255194592909656E-4 22-23 0.0 0.0 0.0 4.514144954421391E-4 1.9006926123879542E-4 24-25 0.0 0.0 0.0 7.365183873003322E-4 2.3758657654849427E-4 26-27 0.0 0.0 0.0 8.553116755745794E-4 2.3758657654849427E-4 28-29 0.0 0.0 0.0 0.00123545019805217 2.3758657654849427E-4 30-31 0.0 0.0 0.0 0.0021857965042461473 2.3758657654849427E-4 32-33 0.0 0.0 0.0 0.004918042134553831 2.3758657654849427E-4 34-35 0.0 0.0 0.0 0.008529358098090944 2.3758657654849427E-4 36-37 0.0 0.0 0.0 0.014255194592909656 2.3758657654849427E-4 38-39 0.0 0.0 0.0 0.02482779724931765 2.3758657654849427E-4 40-41 0.0 0.0 0.0 0.03979575157187279 2.3758657654849427E-4 42-43 0.0 0.0 0.0 0.054739947236773084 2.3758657654849427E-4 44-45 0.0 0.0 0.0 0.07091959309972554 2.3758657654849427E-4 46-47 0.0 0.0 0.0 0.09296762740342582 2.3758657654849427E-4 48-49 0.0 0.0 0.0 0.11846066706707925 2.3758657654849427E-4 50-51 0.0 4.7517315309698855E-5 0.0 0.1460682272620143 2.3758657654849427E-4 52-53 0.0 4.7517315309698855E-5 0.0 0.17374706342991386 2.3758657654849427E-4 54-55 0.0 4.7517315309698855E-5 0.0 0.2035641787867499 2.3758657654849427E-4 56-57 0.0 4.7517315309698855E-5 0.0 0.23934471721495315 2.3758657654849427E-4 58-59 0.0 4.7517315309698855E-5 0.0 0.28291809535394696 2.3758657654849427E-4 60-61 0.0 4.7517315309698855E-5 0.0 0.3291049258349743 2.3758657654849427E-4 62-63 0.0 4.7517315309698855E-5 0.0 0.37764386342383166 2.3758657654849427E-4 64-65 0.0 4.7517315309698855E-5 0.0 0.4296753236879519 2.3758657654849427E-4 66-67 0.0 4.7517315309698855E-5 0.0 0.4918992480860025 2.3758657654849427E-4 68-69 0.0 4.7517315309698855E-5 0.0 0.5638879807801963 2.3758657654849427E-4 70-71 0.0 4.7517315309698855E-5 0.0 0.6484450433738054 3.563798648227414E-4 72-73 0.0 4.7517315309698855E-5 0.0 0.7359719381742706 3.8013852247759084E-4 74-75 0.0 4.7517315309698855E-5 0.0 0.8222158654613742 3.8013852247759084E-4 76-77 0.0 4.7517315309698855E-5 0.0 0.9180345317833818 3.8013852247759084E-4 78-79 0.0 4.7517315309698855E-5 0.0 1.0311732595357748 3.8013852247759084E-4 80-81 0.0 4.7517315309698855E-5 0.0 1.162748705628331 4.038971801324403E-4 82-83 0.0 4.7517315309698855E-5 0.0 1.3040652013593754 4.2765583778728967E-4 84-85 0.0 4.7517315309698855E-5 0.0 1.4445026267571903 4.2765583778728967E-4 86-87 0.0 4.7517315309698855E-5 0.0 1.5967481050094654 4.2765583778728967E-4 88-89 0.0 4.7517315309698855E-5 0.0 1.7666225072416388 4.2765583778728967E-4 90-91 0.0 4.7517315309698855E-5 0.0 1.9637956071192342 4.2765583778728967E-4 92-93 0.0 4.7517315309698855E-5 0.0 2.1768157316526144 4.2765583778728967E-4 94-95 0.0 4.7517315309698855E-5 0.0 2.393447172149531 4.2765583778728967E-4 96-97 0.0 4.7517315309698855E-5 0.0 2.61611331169078 4.2765583778728967E-4 98-99 0.0 4.7517315309698855E-5 0.0 2.8603285537249774 4.2765583778728967E-4 100-101 0.0 4.7517315309698855E-5 0.0 3.144220754042773 4.2765583778728967E-4 102-103 0.0 4.7517315309698855E-5 0.0 3.457478655221963 4.7517315309698855E-4 104-105 0.0 4.7517315309698855E-5 0.0 3.7727085249865047 4.7517315309698855E-4 106-107 0.0 4.7517315309698855E-5 0.0 4.077484585382914 4.7517315309698855E-4 108-109 0.0 4.7517315309698855E-5 0.0 4.406114338064791 5.226904684066874E-4 110-111 0.0 4.7517315309698855E-5 0.0 4.775941603120177 5.226904684066874E-4 112-113 0.0 4.7517315309698855E-5 0.0 5.185873482296949 5.226904684066874E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4425 0.0 53.1141 1 GGTATCA 2315 0.0 52.433052 1 ATCAACG 5290 0.0 43.52926 3 CAACGCA 5355 0.0 43.334232 5 TCAACGC 5320 0.0 43.283794 4 AACGCAG 5520 0.0 41.81439 6 TATCAAC 5885 0.0 39.431576 2 GTACATG 7590 0.0 35.512596 1 ACGCAGA 6565 0.0 34.97725 7 CGCAGAG 6590 0.0 33.94427 8 TACATGG 7600 0.0 33.82176 2 ACATGGG 7890 0.0 32.427795 3 GCAGAGT 7400 0.0 27.737803 9 GTACCGT 235 1.043918E-8 25.31426 6 CATGGGG 4515 0.0 23.984995 4 GAGTACA 5420 0.0 23.602888 1 CATGGGA 4380 0.0 23.365782 4 AGAGTAC 6730 0.0 23.343248 10-11 AGTACAT 5235 0.0 22.504765 2 CAGAGTA 7350 0.0 21.940899 10-11 >>END_MODULE