FastQCFastQC Report
Thu 26 May 2016
SRR1364582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104496
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44050.20931377393922346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37930.18023317696968774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.12321239859804914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30200.061.6127131
GTATCAA55400.051.9948161
TCAACGC70500.040.297034
ATCAACG70250.040.2708783
CAACGCA71300.039.636355
TATCAAC73450.038.678572
AACGCAG74100.038.0583276
ACGCAGA87850.031.966117
CGCAGAG88500.031.6641038
GTACATG77100.031.2499731
TACATGG79050.029.3837052
ACATGGG79050.028.7809643
GCAGAGT101400.026.4623349
TGGTATC18400.025.8950062
GTGGTAT19850.025.8381021
CATGGGA44600.024.7047634
AGAGTAC93650.023.60127410-11
GAGTACT62850.023.19264812-13
CAGAGTA98500.022.49958210-11
GAGTACA60300.021.8573571
Produced by FastQC (version 0.11.5)