##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364582_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104496 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.758684739719154 31.0 30.0 34.0 26.0 34.0 2 29.686887026632505 31.0 30.0 34.0 25.0 34.0 3 29.95934228432841 31.0 30.0 34.0 26.0 34.0 4 33.471294314648254 35.0 33.0 37.0 28.0 37.0 5 32.86816558453901 35.0 33.0 37.0 26.0 37.0 6 32.88963153173016 35.0 33.0 37.0 26.0 37.0 7 32.66650542457671 35.0 33.0 37.0 25.0 37.0 8 32.58991273920216 35.0 33.0 37.0 25.0 37.0 9 33.83241640753891 38.0 33.0 39.0 23.0 39.0 10-11 33.74592800366453 38.0 33.0 39.0 23.0 39.0 12-13 33.62239486318815 38.0 33.0 39.0 22.0 39.0 14-15 34.560447727151775 39.0 33.0 41.0 21.5 41.0 16-17 34.55286372604177 39.0 33.0 41.0 22.0 41.0 18-19 34.551709055279744 39.0 33.5 41.0 21.5 41.0 20-21 34.55875896176566 39.0 34.0 41.0 22.0 41.0 22-23 34.50678951159803 39.0 33.5 41.0 22.0 41.0 24-25 34.45666658430332 39.0 33.0 41.0 21.5 41.0 26-27 34.32889727516707 39.0 33.0 41.0 19.0 41.0 28-29 34.17691646836107 38.5 33.0 41.0 19.0 41.0 30-31 34.04412909314154 38.0 33.0 40.0 18.5 41.0 32-33 33.85942026024283 38.0 33.0 40.0 17.5 41.0 34-35 33.68824958564901 38.0 33.0 40.0 16.0 41.0 36-37 33.498964122526246 38.0 32.5 40.0 16.0 41.0 38-39 33.33691819799135 38.0 32.0 40.0 15.0 41.0 40-41 33.38841081189986 38.0 32.0 40.0 15.0 41.0 42-43 33.266454295945444 38.0 32.0 40.0 11.0 41.0 44-45 33.11684008902844 38.0 32.0 40.0 9.0 41.0 46-47 32.922857301700745 38.0 32.0 40.0 8.0 41.0 48-49 32.67899653883875 38.0 31.0 40.0 4.5 41.0 50-51 32.521882674046424 38.0 31.0 40.0 2.0 41.0 52-53 32.342929851137754 38.0 31.0 40.0 2.0 41.0 54-55 32.16062111783534 37.0 31.0 40.0 2.0 41.0 56-57 31.94029330538048 37.0 30.5 40.0 2.0 41.0 58-59 31.73229908728741 37.0 30.0 40.0 2.0 41.0 60-61 31.49919196805316 36.0 30.0 40.0 2.0 41.0 62-63 31.32429783663167 36.0 30.0 40.0 2.0 41.0 64-65 31.190727138469256 36.0 30.0 39.0 2.0 41.0 66-67 30.892390149470465 35.0 30.0 39.0 2.0 41.0 68-69 30.602911813564866 35.0 29.0 39.0 2.0 41.0 70-71 30.29727854080027 35.0 29.0 38.0 2.0 40.0 72-73 30.004873613444737 35.0 29.0 37.0 2.0 40.0 74-75 29.652048518980315 35.0 29.0 37.0 2.0 39.0 76-77 29.33445513795227 35.0 29.0 36.5 2.0 39.0 78-79 29.0491858858368 34.0 29.0 36.0 2.0 39.0 80-81 28.771666945434916 34.0 29.0 35.5 2.0 37.0 82-83 28.48469847412397 34.0 28.0 35.0 2.0 37.0 84-85 28.258839408580485 34.0 28.0 35.0 2.0 36.5 86-87 28.04442370049646 34.0 28.0 35.0 2.0 36.0 88-89 27.869046793151426 34.0 27.0 35.0 2.0 36.0 90-91 27.72644709232044 34.0 27.0 35.0 2.0 36.0 92-93 27.611363718439 34.0 27.0 35.0 2.0 35.0 94-95 27.49497219286708 34.0 27.0 35.0 2.0 35.0 96-97 27.38159920475021 34.0 27.0 35.0 2.0 35.0 98-99 27.293703337996366 34.0 26.5 35.0 2.0 35.0 100-101 27.184581724080253 34.0 26.0 35.0 2.0 35.0 102-103 25.926465291452203 31.5 24.5 34.0 2.0 35.0 104-105 26.86637774555048 33.5 25.0 35.0 2.0 35.0 106-107 27.030680980149167 34.0 25.0 35.0 2.0 35.0 108-109 27.03305138142339 34.0 26.0 35.0 2.0 35.0 110-111 26.948913421550813 34.0 25.0 35.0 2.0 35.0 112-113 26.862751699219196 34.0 25.0 35.0 2.0 35.0 114-115 26.76409862503896 34.0 25.0 35.0 2.0 35.0 116-117 26.63804374063909 34.0 25.0 35.0 2.0 35.0 118-119 26.501386792847313 34.0 25.0 35.0 2.0 35.0 120-121 26.302526115516493 34.0 25.0 35.0 2.0 35.0 122-123 26.004499889759828 34.0 24.0 35.0 2.0 35.0 124-125 24.22418051638017 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.00857453612295 1101 2 1.0882562318116165 1101 3 1.0143506328306309 1101 4 0.9840245132822574 1101 5 1.1900113084239763 1101 6 1.1155850327538772 1101 7 1.2842956509895345 1101 8 1.290210249616969 1101 9 1.5777521758021251 1101 10-11 1.5799040855100657 1101 12-13 1.5224550054577506 1101 14-15 1.6613152618948206 1101 16-17 1.5871861168307504 1101 18-19 1.5545968331482598 1101 20-21 1.5612486479449075 1101 22-23 1.68054567019243 1101 24-25 1.65002471655977 1101 26-27 1.5705461495672495 1101 28-29 1.673510754756819 1101 30-31 1.6906075871466584 1101 32-33 1.7916681818523514 1101 34-35 1.8611771879235306 1101 36-37 1.8272138110708696 1101 38-39 1.7378908613176094 1101 40-41 1.6024316543045742 1101 42-43 1.672809348242751 1101 44-45 1.7345051333858592 1101 46-47 1.7303228583610348 1101 48-49 1.8546104350651191 1101 50-51 1.8886463308219845 1101 52-53 1.8289236324006097 1101 54-55 1.7831454980901 1101 56-57 1.7138013183054959 1101 58-59 1.8568845864914074 1101 60-61 1.771239903795447 1101 62-63 1.755876405283285 1101 64-65 1.7386365747669004 1101 66-67 1.8194403126060692 1101 68-69 1.8861744931413114 1101 70-71 1.7154397824242125 1101 72-73 1.779494620419186 1101 74-75 1.8858481499803759 1101 76-77 1.8322050351028167 1101 78-79 1.8244278351848102 1101 80-81 1.6753485354111248 1101 82-83 1.827440464516986 1101 84-85 1.801297657964021 1101 86-87 1.8495635753309614 1101 88-89 1.9351565277018956 1101 90-91 1.880753516647328 1101 92-93 1.8286693346277367 1101 94-95 1.8459810487489072 1101 96-97 1.8849038140594345 1101 98-99 1.8795883306131422 1101 100-101 1.8435471001952628 1101 102-103 1.7945878511998217 1101 104-105 1.9167688098020221 1101 106-107 1.8439377285251481 1101 108-109 1.7473315700331753 1101 110-111 1.6709032713715466 1101 112-113 1.77834696409856 1101 114-115 1.848819341070488 1101 116-117 1.7869864303758298 1101 118-119 1.8816244703715732 1101 120-121 1.864452796416284 1101 122-123 1.842762207959126 1101 124-125 2.0996768923486115 1102 1 0.4389724024516397 1102 2 -0.2328031234388277 1102 3 0.3300365769196567 1102 4 0.3715425137052222 1102 5 0.5905441657814094 1102 6 0.29966574638891075 1102 7 0.4536658996064844 1102 8 0.4730254223575088 1102 9 0.9185886434073467 1102 10-11 0.871632933191556 1102 12-13 1.0330479788100178 1102 14-15 1.1499740613086473 1102 16-17 1.052863878060137 1102 18-19 1.1910061308197726 1102 20-21 1.0823361387812298 1102 22-23 1.1467551304749293 1102 24-25 1.1680846427421514 1102 26-27 1.2714254520139576 1102 28-29 0.8606641508677555 1102 30-31 1.077176618722035 1102 32-33 1.2278543004343163 1102 34-35 1.2801001816096473 1102 36-37 0.9688845193478102 1102 38-39 0.4476988219322706 1102 40-41 0.0998119248695204 1102 42-43 0.3519097268961673 1102 44-45 0.05136171214345353 1102 46-47 0.38727365583488194 1102 48-49 0.0958816778060978 1102 50-51 0.12655531500403328 1102 52-53 -0.13004493121981398 1102 54-55 -0.0412125023926464 1102 56-57 -0.07279409271692927 1102 58-59 0.08345806819729518 1102 60-61 0.02289216887825063 1102 62-63 0.10570888084866326 1102 64-65 0.06298299220632941 1102 66-67 -0.05608288598782707 1102 68-69 0.20861456583798343 1102 70-71 -0.24103814664720602 1102 72-73 -0.041177543584751675 1102 74-75 0.1478515456803038 1102 76-77 -0.24848001597093017 1102 78-79 -0.09220012792581045 1102 80-81 -0.1607624241655472 1102 82-83 -0.5116890921962103 1102 84-85 -0.39402916320798553 1102 86-87 -0.04786278453700632 1102 88-89 -0.11865063181454616 1102 90-91 -0.10868087961082651 1102 92-93 -0.0647251920480123 1102 94-95 0.17055062659555276 1102 96-97 -0.10747005967642664 1102 98-99 -0.1160367352420657 1102 100-101 -0.025403281783614062 1102 102-103 -0.06382214817992704 1102 104-105 0.07024628227734553 1102 106-107 0.17984103402679352 1102 108-109 0.1637372134413404 1102 110-111 0.1617428589255283 1102 112-113 0.3660646038778701 1102 114-115 0.29701875698618707 1102 116-117 0.21075235052158092 1102 118-119 0.41054181825819214 1102 120-121 0.6090143707536413 1102 122-123 0.5467668770465259 1102 124-125 0.7527649316980778 1103 1 0.5852928530879424 1103 2 0.17071658510094778 1103 3 0.5205351503629636 1103 4 0.5459444514352896 1103 5 0.6177592189194243 1103 6 0.7481178360659584 1103 7 0.9112536930854915 1103 8 0.9059224919675799 1103 9 1.1851487117551613 1103 10-11 1.147517742043533 1103 12-13 1.175071668640257 1103 14-15 1.2776526792747163 1103 16-17 1.1522796205683505 1103 18-19 1.180069479454815 1103 20-21 1.215318076617045 1103 22-23 1.2398082110744326 1103 24-25 1.1626034281936128 1103 26-27 1.2841604070719512 1103 28-29 1.2288304079863792 1103 30-31 1.277360348799533 1103 32-33 1.3724225262540486 1103 34-35 1.236045187603679 1103 36-37 1.3714877492504343 1103 38-39 1.4991849790068557 1103 40-41 1.2945188783230535 1103 42-43 1.3605895519777391 1103 44-45 1.2655601247639297 1103 46-47 1.2115990724143657 1103 48-49 1.169447737163246 1103 50-51 1.0872126415076195 1103 52-53 1.1727563623124482 1103 54-55 1.1709541905868193 1103 56-57 1.2337574359309151 1103 58-59 1.3355958259085163 1103 60-61 1.2830482176372584 1103 62-63 1.2976718749785157 1103 64-65 1.1270198832569847 1103 66-67 1.079926471801052 1103 68-69 1.1474058602540538 1103 70-71 1.089861535861779 1103 72-73 1.057840579766136 1103 74-75 1.131656142539235 1103 76-77 1.2533208371681361 1103 78-79 1.0083663589828795 1103 80-81 0.9816137501848345 1103 82-83 1.0275392339243936 1103 84-85 1.0882062024052352 1103 86-87 1.1891856519160662 1103 88-89 1.1899528231947194 1103 90-91 1.2373363569105358 1103 92-93 1.184500154053449 1103 94-95 1.154695149592314 1103 96-97 1.1531781265910084 1103 98-99 1.1923377417438026 1103 100-101 1.135554577195382 1103 102-103 0.8662172756981512 1103 104-105 0.960130892461386 1103 106-107 1.0651757328560123 1103 108-109 1.119789768288424 1103 110-111 1.1061011992399372 1103 112-113 1.0438393642865975 1103 114-115 1.1179200786945493 1103 116-117 1.1440600697909389 1103 118-119 1.063727360519696 1103 120-121 1.0815541931006862 1103 122-123 1.1771359159695791 1103 124-125 1.3851916568421139 1104 1 0.7298274836355994 1104 2 0.86581227559385 1104 3 0.5985507293540593 1104 4 0.36086729342526525 1104 5 0.7679631547434269 1104 6 0.8843364030228926 1104 7 0.9540902086075604 1104 8 0.8574702023155467 1104 9 0.9653539287579349 1104 10-11 0.9610392278397626 1104 12-13 0.9710004108331916 1104 14-15 1.1327342304075003 1104 16-17 1.1378690359618417 1104 18-19 1.1189523450984211 1104 20-21 1.0261016227128934 1104 22-23 0.9602670479425512 1104 24-25 1.0788712580801985 1104 26-27 1.0543436120673348 1104 28-29 1.1863017857745888 1104 30-31 1.196526354951331 1104 32-33 1.2373619272119711 1104 34-35 1.330131084394914 1104 36-37 1.3125160540225238 1104 38-39 1.4014759157912096 1104 40-41 1.3452375874944025 1104 42-43 1.3276132951824025 1104 44-45 1.261240339687916 1104 46-47 1.2464692611687624 1104 48-49 1.2452921820755947 1104 50-51 1.2346237682062124 1104 52-53 1.3327157250348591 1104 54-55 0.9585488470364112 1104 56-57 1.1888992274470773 1104 58-59 1.3366937497208546 1104 60-61 1.2736139776171527 1104 62-63 1.3249543806073198 1104 64-65 1.312392176320735 1104 66-67 1.3518502420211078 1104 68-69 1.0845792087378925 1104 70-71 0.6078419341834689 1104 72-73 0.5686449494779282 1104 74-75 1.0417858235257214 1104 76-77 1.1925986734534746 1104 78-79 1.3120727306524849 1104 80-81 1.2622663983528 1104 82-83 1.141116534862583 1104 84-85 1.149221627583504 1104 86-87 1.198411031246696 1104 88-89 1.2712484339619046 1104 90-91 1.2398962163306066 1104 92-93 1.169188997127609 1104 94-95 1.167255073249759 1104 96-97 1.206552069841571 1104 98-99 1.2816888978559717 1104 100-101 1.215173414020672 1104 102-103 1.0014042974642692 1104 104-105 1.1111574235681552 1104 106-107 1.1823652263336015 1104 108-109 1.1461913296848998 1104 110-111 1.1651102770517419 1104 112-113 1.1431673878060415 1104 114-115 1.1527180896198992 1104 116-117 1.188988850169416 1104 118-119 1.2635554720086581 1104 120-121 1.1497441301730404 1104 122-123 1.2170572144781673 1104 124-125 1.6956256445520772 1105 1 0.4534146557834049 1105 2 0.21134700629415448 1105 3 0.5216360799948276 1105 4 0.5449166828526941 1105 5 0.20108280645617072 1105 6 0.6385377210229137 1105 7 0.7646893161374209 1105 8 0.7792499015384564 1105 9 1.1930046947702593 1105 10-11 1.0301594451750944 1105 12-13 1.0638129940553824 1105 14-15 1.1837889001028756 1105 16-17 1.2120164281985808 1105 18-19 1.1648594144405848 1105 20-21 1.1726292801145064 1105 22-23 1.2105748758009511 1105 24-25 1.136138905265831 1105 26-27 1.364616883365514 1105 28-29 1.2841406567861995 1105 30-31 1.2601042071006319 1105 32-33 1.4400687010394009 1105 34-35 1.4151139285362646 1105 36-37 1.5779814320821544 1105 38-39 1.513867149208643 1105 40-41 1.2932921243004145 1105 42-43 1.3842871096060279 1105 44-45 1.4311719628704225 1105 46-47 1.4548322610053575 1105 48-49 1.4687281459857502 1105 50-51 1.2526078346104441 1105 52-53 1.3465174132799334 1105 54-55 1.349606039678278 1105 56-57 1.2328325613363411 1105 58-59 0.8992765727134504 1105 60-61 1.0949982821577322 1105 62-63 1.1998831676928141 1105 64-65 1.0633901329469921 1105 66-67 1.1614885629168334 1105 68-69 1.3064061289316982 1105 70-71 1.281879422116532 1105 72-73 1.176866579148772 1105 74-75 1.1539794527487537 1105 76-77 1.255469807840825 1105 78-79 1.0958971573519278 1105 80-81 1.070469105925028 1105 82-83 1.1371936813143009 1105 84-85 1.106918672453805 1105 86-87 1.1488154389867802 1105 88-89 1.1300820821904196 1105 90-91 0.9456347840741159 1105 92-93 1.0203591954166562 1105 94-95 0.9708756256472952 1105 96-97 1.0281038167691037 1105 98-99 0.9440584679987616 1105 100-101 1.0343817659316095 1105 102-103 0.6754136195918399 1105 104-105 0.8526648344129555 1105 106-107 0.8445171879067459 1105 108-109 1.0123570471713137 1105 110-111 0.9015466896631388 1105 112-113 0.9138346574722966 1105 114-115 0.9241518683958176 1105 116-117 0.612935719613322 1105 118-119 0.7836504657341159 1105 120-121 0.8674507700465597 1105 122-123 0.7891515190950358 1105 124-125 1.2749251614131474 1106 1 -0.06842575347712909 1106 2 -0.380431078422923 1106 3 0.10395271503698567 1106 4 -7.068234497822345E-4 1106 5 0.37134084599275496 1106 6 0.5919706052058018 1106 7 0.5575746706477318 1106 8 0.5657017045932662 1106 9 0.6275302401443454 1106 10-11 0.5961961845277983 1106 12-13 0.7065812783775911 1106 14-15 0.7733575252924609 1106 16-17 0.6637846090889497 1106 18-19 0.6698888101332514 1106 20-21 0.7296655737034712 1106 22-23 0.6675366160918657 1106 24-25 0.6673472636562039 1106 26-27 0.8301548616118879 1106 28-29 0.9169012249107027 1106 30-31 0.8645878396997801 1106 32-33 0.8357847467947153 1106 34-35 0.9135846934833012 1106 36-37 0.8993074739290208 1106 38-39 0.8363930460678901 1106 40-41 0.8501381673921244 1106 42-43 0.7250427294784885 1106 44-45 0.6505983372552109 1106 46-47 0.5980969265864431 1106 48-49 0.8934846921680588 1106 50-51 0.8838899682718306 1106 52-53 0.717036824139548 1106 54-55 0.8047938325253696 1106 56-57 0.3671982908007685 1106 58-59 0.7303345430513879 1106 60-61 0.7759629350157908 1106 62-63 0.7418458278862907 1106 64-65 0.8715317741447137 1106 66-67 0.7303941168400563 1106 68-69 0.8429646132251634 1106 70-71 0.8191686111102854 1106 72-73 0.8836956429442431 1106 74-75 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2216 72-73 -2.9820226098628453 2216 74-75 -2.904188468349794 2216 76-77 -3.1421481099725916 2216 78-79 -3.123989542263349 2216 80-81 -3.0651482424663534 2216 82-83 -2.7821688580159716 2216 84-85 -2.8465931284528914 2216 86-87 -3.002191634986982 2216 88-89 -2.9249875990062435 2216 90-91 -2.9168308133445073 2216 92-93 -2.8947932709837936 2216 94-95 -2.8620194473920293 2216 96-97 -2.8576891034169094 2216 98-99 -2.8135239704416044 2216 100-101 -3.025330894948368 2216 102-103 -2.9005925418466063 2216 104-105 -2.9152423935967704 2216 106-107 -3.1088902189598997 2216 108-109 -2.9679993526213586 2216 110-111 -3.056064949907313 2216 112-113 -3.1042174811454686 2216 114-115 -3.2192994131687804 2216 116-117 -3.1449277797041013 2216 118-119 -3.185652576293613 2216 120-121 -3.443123689185011 2216 122-123 -3.4165916131641865 2216 124-125 -3.573215821642897 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80901.0 3 25439.0 4 11963.0 5 9180.0 6 9709.0 7 11049.0 8 12285.0 9 13346.0 10 14289.0 11 15280.0 12 16896.0 13 18185.0 14 20221.0 15 18008.0 16 13578.0 17 12100.0 18 11244.0 19 10613.0 20 10640.0 21 10940.0 22 12603.0 23 15722.0 24 20346.0 25 26711.0 26 33243.0 27 37408.0 28 42400.0 29 50828.0 30 59162.0 31 69101.0 32 83742.0 33 103708.0 34 133187.0 35 181338.0 36 293190.0 37 438774.0 38 156564.0 39 603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.314902150487 19.312049643234438 13.755962938810223 24.617085267468337 2 15.667599273175146 20.247793295877017 39.43666322007739 24.64794421087044 3 19.63695820757084 24.597148200804373 28.752917563160015 27.012976028464774 4 13.080370074586648 16.89745988726148 36.04778758620558 33.97438245194629 5 13.28085204248428 38.38847876166075 34.2944343918924 14.036234803962563 6 32.62690924572914 36.91073777284443 16.804688628536237 13.657664352890192 7 28.749866354947006 32.02786469656039 20.561095390493026 18.66117355799958 8 25.89263566665051 36.747725940012906 18.820073883783678 18.539564509552907 9 25.91969390184388 16.190471212299048 19.288470046183683 38.601364839673394 10-11 25.133482998068647 26.841457147520657 27.09465490668366 20.93040494772703 12-13 25.846235868350426 23.824207791319157 27.787413233382246 22.542143106948174 14-15 22.555263244801427 25.41065067613592 25.55393917449574 26.480146904566915 16-17 22.466974168075957 28.562967067064736 26.18619056692622 22.783868197933085 18-19 22.294310068074665 27.27636241989609 28.30888677063029 22.120440741398955 20-21 23.0280089257543 26.427270118110386 28.483415317476307 22.061305638659007 22-23 22.67961070013913 26.357284594506236 28.361707506215268 22.601397199139367 24-25 22.489992853395776 26.467976180520182 28.506920421801706 22.535110544282336 26-27 22.372363739346618 26.68225551390927 28.406064920056867 22.539315826687243 28-29 22.366138971041046 26.73364549041671 28.53417160213182 22.366043936410428 30-31 22.480631942279768 26.54343367723198 28.430156198918887 22.545778181569364 32-33 22.362931552257642 26.561276429130775 28.493805642776227 22.581986375835356 34-35 22.610401730390553 26.57458127741749 28.342367958884218 22.472649033307736 36-37 22.64684751123309 26.460349651412972 28.25001805657982 22.642784780774114 38-39 22.48255639354981 26.70361454714098 28.310650150914995 22.50317890839422 40-41 22.6556857318807 26.563153363085508 28.21989207867347 22.561268826360326 42-43 22.56794500916134 26.563771088184534 28.054270476161513 22.814013426492615 44-45 22.68279436026488 26.700502162988194 28.027779572876355 22.58892390387057 46-47 22.67250686150033 26.877717990435713 27.67016425785556 22.779610890208392 48-49 22.85254996920878 26.82609042735173 27.530962282655803 22.790397320783693 50-51 22.76971434952328 27.02964369124184 27.453807207904983 22.746834751329892 52-53 22.84544613056998 27.0505859835324 27.28565414236948 22.818313743528144 54-55 22.7816066174514 26.965244885236178 27.360493913982253 22.892654583330167 56-57 22.65954173443312 27.172484582513107 27.33167882205767 22.836294860996105 58-59 22.859326635868847 26.94109893284701 27.359706302465504 22.839868128818644 60-61 22.909309402346214 26.966337783488303 27.468263185104654 22.65608962906083 62-63 22.754830610274386 27.01031505880743 27.555623769301533 22.679230561616652 64-65 22.598966970619962 26.867340780901788 27.530494324040504 23.00319792443775 66-67 22.653951349871893 27.077124404132867 27.35272483292912 22.91619941306612 68-69 22.551319306693696 27.028591888174624 27.488322023021674 22.93176678211001 70-71 22.564096106621253 26.865339729797537 27.496227125164406 23.074337038416797 72-73 22.671651549824755 27.011669302293758 27.319343918924055 22.997335228957432 74-75 22.64307214424144 27.128996520028902 27.154679927953406 23.07325140777625 76-77 22.73487333784431 27.026423428697417 27.203425428226048 23.035277805232226 78-79 22.701349871893317 27.000455215880663 27.21580368886422 23.082391223361792 80-81 22.565157571093675 27.129270235678927 27.21164156047925 23.09393063274815 82-83 22.717363207152687 27.137305083972603 27.126304825478403 23.01902688339631 84-85 22.755828473895885 27.016421033824727 27.076482920376183 23.1512675719032 86-87 22.558298994153468 27.03661589283135 27.339134880750542 23.065950232264637 88-89 22.777027388228586 26.940394518790427 27.15619784149159 23.126380251489394 90-91 22.664547711185957 27.179975633120705 27.208319711702945 22.94715694399039 92-93 22.587535320978088 27.095267862085613 27.181870041609436 23.135326775326863 94-95 22.542071830975207 27.036639651489004 27.167407303221292 23.253881214314497 96-97 22.675304099311468 26.939276130300915 27.184532456664336 23.200887313723282 98-99 22.45501306419143 26.965194389988863 27.11005639805618 23.469736147763527 100-101 22.39861873287701 26.955450713953255 27.263631804022392 23.38229874914734 102-103 22.409165900053978 26.75790307988231 27.287436041693592 23.54549497837012 104-105 22.45143255202196 26.796130760048964 27.28066482426196 23.471771863667122 106-107 22.355164461077866 26.85060736353595 27.301220809379302 23.49300736600689 108-109 22.285055967095204 26.81956316846484 27.13460304381739 23.760777820622568 110-111 22.22802990651186 26.90540684055982 27.1699125418183 23.696650711110024 112-113 22.242237571371007 26.90031247386548 27.24303111053668 23.614418844226837 114-115 22.25718176703591 26.987863127323596 27.064342246314553 23.690612859325938 116-117 22.01938136256852 26.879880028282304 27.27845526910006 23.822283340049115 118-119 22.0704862351841 26.911122663098435 27.14878051561989 23.869610586097572 120-121 22.078628673958434 26.98826405077944 27.20620449575523 23.726902779506897 122-123 22.055153684495643 26.944474474224744 27.14303534155344 23.857336499726166 124-125 21.94673233339944 26.98237869749883 27.05751878858955 24.01337018051218 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 201.0 1 173.0 2 225.5 3 322.5 4 270.0 5 228.0 6 394.5 7 964.5 8 1595.0 9 1807.0 10 1482.5 11 1042.0 12 974.0 13 1040.5 14 1129.0 15 1242.5 16 1383.5 17 1548.0 18 1814.5 19 2181.0 20 2570.0 21 3109.0 22 3815.0 23 4797.0 24 6132.0 25 7762.0 26 10122.5 27 13265.0 28 16193.5 29 19482.0 30 23739.5 31 27685.5 32 31805.5 33 35971.0 34 40515.0 35 44970.5 36 49642.0 37 54219.5 38 56919.5 39 59673.5 40 62138.5 41 64248.5 42 66477.5 43 69255.5 44 72510.0 45 74619.5 46 76076.0 47 76986.0 48 78283.5 49 79532.5 50 78084.0 51 75546.5 52 75001.0 53 75090.5 54 73715.5 55 69475.0 56 63139.0 57 57413.0 58 53507.5 59 48623.5 60 41752.0 61 34168.0 62 26606.5 63 20884.5 64 15671.5 65 11136.5 66 8410.0 67 6585.5 68 4835.0 69 3528.0 70 2611.5 71 1820.5 72 1318.5 73 1094.5 74 808.0 75 459.5 76 290.5 77 169.5 78 105.0 79 68.0 80 45.5 81 31.0 82 18.5 83 15.5 84 9.5 85 5.0 86 4.5 87 3.5 88 2.0 89 2.5 90 2.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10144946818620706 2 0.0 3 0.0 4 0.10021401798815488 5 0.0 6 0.0 7 0.0019482099276976529 8 0.0052269046840668736 9 0.0034687640176080167 10-11 6.890010719906335E-4 12-13 0.0 14-15 2.3758657654849427E-5 16-17 3.3262120716789196E-4 18-19 0.0019957272430073517 20-21 0.0011404155674327725 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.7517315309698855E-5 52-53 0.0 54-55 0.0 56-57 0.00705632132349028 58-59 8.553116755745794E-4 60-61 0.0 62-63 0.0 64-65 2.8510389185819313E-4 66-67 0.0 68-69 2.3758657654849427E-5 70-71 0.0 72-73 0.0 74-75 0.001164174225087622 76-77 0.0 78-79 0.0 80-81 7.127597296454828E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0015918300628749117 90-91 0.0 92-93 0.002162037846591298 94-95 0.0 96-97 0.007935391656719708 98-99 0.003777626567121059 100-101 0.002447141738449491 102-103 0.0 104-105 0.0 106-107 0.001496795432255514 108-109 2.3758657654849427E-5 110-111 2.1382791889364486E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010453809368133748 122-123 0.004918042134553832 124-125 0.08348792299914089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2104496.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.74751776550329 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09975322030326 50.260606882034075 2 11.57513052332152 14.526214163002727 3 3.6205594264600878 6.815433507985942 4 1.5961004976191189 4.006053773195372 5 0.844922699160266 2.650840103801789 6 0.5073157837541242 1.909968369229928 7 0.3416292261369589 1.5005470155370777 8 0.23753290486102585 1.1923680134127057 9 0.16383327747094667 0.9252118339819969 >10 0.931025096194963 10.663309923206588 >50 0.05915928686399011 2.5414771707918162 >100 0.022506357208883557 2.3427131042260094 >500 2.2787170499075405E-4 0.09541788160679161 >1k 3.038289399875626E-4 0.5698382579872586 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4405 0.20931377393922346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3793 0.18023317696968774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2593 0.12321239859804914 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3758657654849427E-5 0.0 12-13 0.0 0.0 0.0 4.7517315309698855E-5 0.0 14-15 0.0 0.0 0.0 4.7517315309698855E-5 4.7517315309698855E-5 16-17 0.0 0.0 0.0 9.503463061939771E-5 4.7517315309698855E-5 18-19 0.0 0.0 0.0 1.4255194592909656E-4 4.7517315309698855E-5 20-21 0.0 0.0 0.0 3.3262120716789196E-4 4.7517315309698855E-5 22-23 0.0 0.0 0.0 5.939664413712357E-4 4.7517315309698855E-5 24-25 0.0 0.0 0.0 9.741049638488264E-4 4.7517315309698855E-5 26-27 0.0 0.0 0.0 0.0011404155674327725 4.7517315309698855E-5 28-29 0.0 0.0 0.0 0.0015918300628749117 4.7517315309698855E-5 30-31 0.0 0.0 0.0 0.0026372109996882862 4.7517315309698855E-5 32-33 0.0 0.0 0.0 0.005583284548889615 4.7517315309698855E-5 34-35 0.0 0.0 0.0 0.009408428431320372 4.7517315309698855E-5 36-37 0.0 0.0 0.0 0.01532433418737788 4.7517315309698855E-5 38-39 0.0 0.0 0.0 0.026419627312192565 4.7517315309698855E-5 40-41 0.0 0.0 0.0 0.04183899613018984 4.7517315309698855E-5 42-43 0.0 0.0 0.0 0.05704453702929348 4.7517315309698855E-5 44-45 0.0 0.0 0.0 0.07312914826162653 4.7517315309698855E-5 46-47 0.0 0.0 0.0 0.0950821479347074 4.7517315309698855E-5 48-49 0.0 0.0 0.0 0.1207414982019448 4.7517315309698855E-5 50-51 0.0 0.0 0.0 0.14818274779329588 4.7517315309698855E-5 52-53 2.3758657654849427E-5 0.0 0.0 0.17576654933057606 4.7517315309698855E-5 54-55 4.7517315309698855E-5 0.0 0.0 0.204965939588386 4.7517315309698855E-5 56-57 4.7517315309698855E-5 0.0 0.0 0.24008123560225347 4.7517315309698855E-5 58-59 4.7517315309698855E-5 0.0 0.0 0.28289433669629216 4.7517315309698855E-5 60-61 4.7517315309698855E-5 0.0 0.0 0.3296513749610358 4.7517315309698855E-5 62-63 4.7517315309698855E-5 0.0 0.0 0.37807151926161897 4.7517315309698855E-5 64-65 4.7517315309698855E-5 0.0 0.0 0.42946149576905823 4.7517315309698855E-5 66-67 4.7517315309698855E-5 0.0 0.0 0.49104393641042793 4.7517315309698855E-5 68-69 4.7517315309698855E-5 0.0 0.0 0.5630326691046217 4.7517315309698855E-5 70-71 4.7517315309698855E-5 0.0 0.0 0.646924489283895 4.7517315309698855E-5 72-73 4.7517315309698855E-5 0.0 0.0 0.733548555093476 9.503463061939771E-5 74-75 4.7517315309698855E-5 0.0 0.0 0.8194836198310664 9.503463061939771E-5 76-77 4.7517315309698855E-5 0.0 0.0 0.9152072515224547 9.503463061939771E-5 78-79 4.7517315309698855E-5 0.0 0.0 1.0278232888064411 9.503463061939771E-5 80-81 4.7517315309698855E-5 0.0 0.0 1.158163284700945 1.1879328827424714E-4 82-83 4.7517315309698855E-5 0.0 0.0 1.2990521245942022 1.9006926123879542E-4 84-85 4.7517315309698855E-5 0.0 0.0 1.4399172058298044 1.9006926123879542E-4 86-87 4.7517315309698855E-5 0.0 0.0 1.591639993613673 2.613452342033437E-4 88-89 4.7517315309698855E-5 0.0 0.0 1.7625597767826595 3.3262120716789196E-4 90-91 4.7517315309698855E-5 0.0 0.0 1.9593764967954321 3.3262120716789196E-4 92-93 4.7517315309698855E-5 0.0 0.0 2.1723253453558478 3.3262120716789196E-4 94-95 4.7517315309698855E-5 0.0 0.0 2.3898596148436493 3.3262120716789196E-4 96-97 4.7517315309698855E-5 0.0 0.0 2.6124544784119332 3.3262120716789196E-4 98-99 4.7517315309698855E-5 0.0 0.0 2.856527168500201 3.3262120716789196E-4 100-101 4.7517315309698855E-5 0.0 0.0 3.1409895766017133 3.3262120716789196E-4 102-103 4.7517315309698855E-5 0.0 0.0 3.455364134690681 3.563798648227414E-4 104-105 4.7517315309698855E-5 0.0 0.0 3.7700713139868167 3.8013852247759084E-4 106-107 4.7517315309698855E-5 0.0 0.0 4.075797720689419 3.8013852247759084E-4 108-109 4.7517315309698855E-5 0.0 0.0 4.405496612965765 3.8013852247759084E-4 110-111 4.7517315309698855E-5 0.0 0.0 4.776559328219204 4.038971801324403E-4 112-113 4.7517315309698855E-5 0.0 0.0 5.187964244170576 4.2765583778728967E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3020 0.0 61.612713 1 GTATCAA 5540 0.0 51.994816 1 TCAACGC 7050 0.0 40.29703 4 ATCAACG 7025 0.0 40.270878 3 CAACGCA 7130 0.0 39.63635 5 TATCAAC 7345 0.0 38.67857 2 AACGCAG 7410 0.0 38.058327 6 ACGCAGA 8785 0.0 31.96611 7 CGCAGAG 8850 0.0 31.664103 8 GTACATG 7710 0.0 31.249973 1 TACATGG 7905 0.0 29.383705 2 ACATGGG 7905 0.0 28.780964 3 GCAGAGT 10140 0.0 26.462334 9 TGGTATC 1840 0.0 25.895006 2 GTGGTAT 1985 0.0 25.838102 1 CATGGGA 4460 0.0 24.704763 4 AGAGTAC 9365 0.0 23.601274 10-11 GAGTACT 6285 0.0 23.192648 12-13 CAGAGTA 9850 0.0 22.499582 10-11 GAGTACA 6030 0.0 21.857357 1 >>END_MODULE