FastQCFastQC Report
Thu 26 May 2016
SRR1364581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771574
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43560.15716701051460288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.11152507564293791No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81950.054.59591
GGTATCA43250.054.4753721
ATCAACG97100.044.790043
TCAACGC100200.043.107444
CAACGCA100750.042.9311645
AACGCAG103200.041.7928966
TATCAAC105200.041.5675962
GTACATG136250.037.3353961
TACATGG138950.035.5816272
ACGCAGA123350.034.6763887
CGCAGAG122150.034.38648
ACATGGG142700.033.7293433
GCAGAGT135150.029.979359
CATGGGA87750.029.0188334
GAGTACT57600.025.934612-13
CATGGGG59900.025.4270764
AGTACTT62000.023.854112-13
AGAGTAC129200.023.23636410-11
CAGAGTA134450.023.21422810-11
ATGGGAG41500.023.0813455
Produced by FastQC (version 0.11.5)