##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364581_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2771574 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.468745918384283 31.0 28.0 31.0 10.0 34.0 2 27.52749773233549 31.0 27.0 33.0 10.0 34.0 3 27.644569114878404 31.0 27.0 34.0 10.0 34.0 4 30.936001347970503 35.0 32.0 37.0 12.0 37.0 5 31.01300632781228 35.0 32.0 37.0 12.0 37.0 6 31.017724224574195 35.0 32.0 37.0 11.0 37.0 7 30.774696977241092 35.0 31.0 37.0 10.0 37.0 8 30.65329953304512 35.0 31.0 37.0 10.0 37.0 9 31.677581403202655 37.0 31.0 39.0 8.0 39.0 10-11 31.590824744351046 37.0 30.5 39.0 8.0 39.0 12-13 31.499858924928578 37.0 30.0 39.0 7.5 39.0 14-15 32.13422336910362 38.0 30.5 40.0 4.5 41.0 16-17 32.249517241827206 38.0 31.0 40.0 2.0 41.0 18-19 32.050052064278276 38.0 30.0 40.0 2.0 41.0 20-21 31.761868346289873 38.0 30.0 40.0 2.0 41.0 22-23 31.65645477984712 38.0 29.5 40.0 2.0 41.0 24-25 31.472894283176274 37.0 29.0 40.0 2.0 41.0 26-27 31.366557595070528 37.0 29.0 40.0 2.0 41.0 28-29 31.40898294615262 37.0 29.5 40.0 2.0 41.0 30-31 31.348946483117537 37.0 29.5 40.0 2.0 41.0 32-33 31.32397006177717 37.0 30.0 40.0 2.0 41.0 34-35 30.848221624246726 37.0 28.5 40.0 2.0 41.0 36-37 30.699343766394115 37.0 28.0 40.0 2.0 41.0 38-39 30.49088730807837 36.0 27.5 40.0 2.0 41.0 40-41 30.695818693637623 37.0 28.5 40.0 2.0 41.0 42-43 30.57506384458795 37.0 28.0 40.0 2.0 41.0 44-45 30.419322558228643 36.0 28.0 40.0 2.0 41.0 46-47 30.375125289817266 36.0 28.0 40.0 2.0 41.0 48-49 30.21825702651273 36.0 28.0 40.0 2.0 41.0 50-51 30.057681844323838 36.0 28.0 40.0 2.0 41.0 52-53 29.97871227685063 36.0 27.5 40.0 2.0 41.0 54-55 30.000204216088044 36.0 28.0 40.0 2.0 41.0 56-57 29.876648251138164 36.0 28.0 40.0 2.0 41.0 58-59 29.68058457035605 35.5 27.5 40.0 2.0 41.0 60-61 29.48341429815693 35.0 27.0 39.5 2.0 41.0 62-63 29.21255791835253 35.0 26.0 39.0 2.0 41.0 64-65 28.93882284939893 35.0 26.0 39.0 2.0 41.0 66-67 28.705123514652684 34.5 26.0 39.0 2.0 40.0 68-69 28.669690940959903 35.0 26.0 38.5 2.0 40.0 70-71 28.446541026867763 34.0 26.0 38.0 2.0 40.0 72-73 28.173541280153444 34.0 26.0 37.0 2.0 40.0 74-75 27.879208889966495 34.0 26.0 37.0 2.0 39.0 76-77 27.577805788335436 34.0 26.0 36.0 2.0 39.0 78-79 27.2726365956673 34.0 25.5 36.0 2.0 38.5 80-81 26.971893588264287 34.0 25.0 35.0 2.0 37.0 82-83 26.717357357227336 34.0 24.5 35.0 2.0 37.0 84-85 26.473126100908726 33.0 24.0 35.0 2.0 36.5 86-87 26.265126422747507 33.0 23.5 35.0 2.0 36.0 88-89 26.064328428539163 33.0 23.0 35.0 2.0 36.0 90-91 25.92358205120989 33.0 23.0 35.0 2.0 35.5 92-93 25.79131803805347 33.0 21.5 35.0 2.0 35.0 94-95 25.65983679310024 33.0 20.0 35.0 2.0 35.0 96-97 25.5430177942209 33.0 20.0 35.0 2.0 35.0 98-99 25.442865137283 33.0 20.0 35.0 2.0 35.0 100-101 25.34179657479829 33.0 19.0 35.0 2.0 35.0 102-103 24.240598302625152 31.0 17.5 34.0 2.0 35.0 104-105 25.058124733454708 32.5 18.0 35.0 2.0 35.0 106-107 25.196910311613546 33.0 18.0 35.0 2.0 35.0 108-109 25.14145806678804 33.0 18.0 35.0 2.0 35.0 110-111 25.056973221714447 33.0 16.5 35.0 2.0 35.0 112-113 24.95555738363832 33.0 12.5 35.0 2.0 35.0 114-115 24.826352823341537 33.0 7.0 35.0 2.0 35.0 116-117 24.70689344755002 33.0 2.0 35.0 2.0 35.0 118-119 24.546731568415638 33.0 2.0 35.0 2.0 35.0 120-121 24.355478331085514 33.0 2.0 35.0 2.0 35.0 122-123 24.102931944086645 33.0 2.0 35.0 2.0 35.0 124-125 22.69959416562574 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0232604879912621 1101 2 0.9834789502294754 1101 3 0.9217572658004762 1101 4 1.0889673093914638 1101 5 1.134588901696823 1101 6 1.12307748878413 1101 7 1.187176836627728 1101 8 1.1688846073572918 1101 9 1.4121742347535324 1101 10-11 1.4959236981663508 1101 12-13 1.319191283959693 1101 14-15 1.6020673546551194 1101 16-17 1.3805850058405582 1101 18-19 1.7596269625952203 1101 20-21 2.123845836959134 1101 22-23 1.9537471724071622 1101 24-25 2.0790147521834257 1101 26-27 2.1301691522522184 1101 28-29 1.9267103029625225 1101 30-31 1.86740197168562 1101 32-33 1.9372075988964568 1101 34-35 2.237581987447914 1101 36-37 2.1388047199592926 1101 38-39 1.948265842331999 1101 40-41 1.9111830695094518 1101 42-43 1.9378767335669451 1101 44-45 1.8104133465567678 1101 46-47 1.663670952610044 1101 48-49 1.654332968399224 1101 50-51 1.6614742277335672 1101 52-53 1.4666507992962572 1101 54-55 1.4775879806904442 1101 56-57 1.501255941793815 1101 58-59 1.48256922510474 1101 60-61 1.515770072032236 1101 62-63 1.40152250665043 1101 64-65 1.4103412428675988 1101 66-67 1.2685017419805078 1101 68-69 1.1656607413844107 1101 70-71 1.1155069973659018 1101 72-73 1.0421053804202067 1101 74-75 1.12116533523918 1101 76-77 1.2504825295571536 1101 78-79 1.1980201205357304 1101 80-81 1.3099997642789987 1101 82-83 1.199568162870417 1101 84-85 1.277220655246012 1101 86-87 1.3498578828566146 1101 88-89 1.3066770185009027 1101 90-91 1.1797810793277428 1101 92-93 1.153995092445129 1101 94-95 1.2655648876648513 1101 96-97 1.2240281971310125 1101 98-99 1.254996994673693 1101 100-101 1.2767845377732918 1101 102-103 1.3031428196453199 1101 104-105 1.3093882585087364 1101 106-107 1.2873204545387438 1101 108-109 1.3483283376979607 1101 110-111 1.1723431720072668 1101 112-113 1.18329080575608 1101 114-115 1.4230431350062425 1101 116-117 1.5010014138035395 1101 118-119 1.4869441415328666 1101 120-121 1.6305790121771224 1101 122-123 1.5948519261694187 1101 124-125 1.7637978567945929 1102 1 -0.3064314267638508 1102 2 -0.07965654969029146 1102 3 0.05993577772902725 1102 4 0.19734587234248124 1102 5 0.1257530398528104 1102 6 -0.496257022501144 1102 7 -1.0538363906235766 1102 8 -0.25862725815991894 1102 9 0.02751443080063609 1102 10-11 -0.04296853459737804 1102 12-13 0.034684983942348424 1102 14-15 0.2445564966657372 1102 16-17 0.13573487865981804 1102 18-19 0.3174388915500188 1102 20-21 0.6699128202724154 1102 22-23 0.5458153965337402 1102 24-25 0.7612577640525231 1102 26-27 0.35349694854790314 1102 28-29 0.8157090246070275 1102 30-31 0.6667128191631981 1102 32-33 0.48522188728234994 1102 34-35 0.8350261670015975 1102 36-37 1.0224083658480119 1102 38-39 1.0041839856729986 1102 40-41 0.8302388605331679 1102 42-43 0.6617988315856103 1102 44-45 0.6239277170989546 1102 46-47 0.28935568301079684 1102 48-49 -0.00625186221299856 1102 50-51 0.30624069692827405 1102 52-53 0.1735426213657938 1102 54-55 0.025814164225693048 1102 56-57 0.05105330996351398 1102 58-59 0.22557000615707068 1102 60-61 0.18781094797727604 1102 62-63 0.0778228505195031 1102 64-65 0.2402445289274482 1102 66-67 0.25108731330902856 1102 68-69 -0.09938630248181468 1102 70-71 -0.0446621625392396 1102 72-73 0.1449663752814665 1102 74-75 -0.163082272842626 1102 76-77 0.025823556771054967 1102 78-79 -0.10401428243593358 1102 80-81 -0.4459853787890822 1102 82-83 -0.31411046788355534 1102 84-85 -0.6839769513428386 1102 86-87 -0.17174581855963567 1102 88-89 0.013670990271613448 1102 90-91 -0.41151691859049677 1102 92-93 0.0085182899438081 1102 94-95 -0.07255167090647774 1102 96-97 0.11577815072710607 1102 98-99 0.15620915366612564 1102 100-101 0.07726827335817532 1102 102-103 -0.008001939751974163 1102 104-105 0.2611901879681682 1102 106-107 0.15262280281195473 1102 108-109 -0.3168685082684952 1102 110-111 -0.05981585883827378 1102 112-113 -0.3643652602162142 1102 114-115 -0.2785329737676463 1102 116-117 -0.4530722551640274 1102 118-119 -0.07916897852236815 1102 120-121 0.3268490987808619 1102 122-123 0.22296240639699505 1102 124-125 0.4934464495995172 1103 1 0.4391512652464371 1103 2 0.4296802638461408 1103 3 0.274768549720239 1103 4 0.41789955282252933 1103 5 0.48975058671100413 1103 6 0.5612268898232387 1103 7 0.5772411855589894 1103 8 0.6221327553925668 1103 9 0.822378297957929 1103 10-11 0.9650795826339973 1103 12-13 0.8872738867221379 1103 14-15 1.1769908267207327 1103 16-17 1.0619378684706646 1103 18-19 1.0878384833226704 1103 20-21 1.4064876534930093 1103 22-23 1.3810411198150945 1103 24-25 1.491471310164627 1103 26-27 1.3689511221354245 1103 28-29 1.3587170392267147 1103 30-31 1.265561802195439 1103 32-33 1.2277964407172846 1103 34-35 1.5972819595682672 1103 36-37 1.5475168270622852 1103 38-39 1.5516634060798822 1103 40-41 1.2860680236434838 1103 42-43 1.132784409047872 1103 44-45 0.9143899773185105 1103 46-47 1.0242192786461395 1103 48-49 1.0118358381967596 1103 50-51 1.0468450044469542 1103 52-53 0.9911979525812562 1103 54-55 0.85896265603931 1103 56-57 0.9172330669395627 1103 58-59 0.8930409385516462 1103 60-61 0.7151058987314549 1103 62-63 0.5714094134883929 1103 64-65 0.8518024805658477 1103 66-67 0.8775222182822446 1103 68-69 0.8166280917231994 1103 70-71 0.8144474579557084 1103 72-73 0.8101114270300087 1103 74-75 0.8567138023665954 1103 76-77 0.7172160452933483 1103 78-79 0.8164359245700439 1103 80-81 0.741130520946129 1103 82-83 0.7904902059630992 1103 84-85 0.7319599410530131 1103 86-87 0.7280283460086387 1103 88-89 0.7670468640115438 1103 90-91 0.8601621613514681 1103 92-93 0.8746709537512274 1103 94-95 0.7795333520607386 1103 96-97 0.9266462595294946 1103 98-99 0.8584056481996818 1103 100-101 0.9195480483963401 1103 102-103 0.7485078285453604 1103 104-105 0.850471681858334 1103 106-107 0.8382010619312545 1103 108-109 0.955572220260315 1103 110-111 0.9797420857169392 1103 112-113 0.9823700719908928 1103 114-115 1.097815103207644 1103 116-117 1.1392586368328033 1103 118-119 1.1196061623444038 1103 120-121 1.100113247140456 1103 122-123 1.148345488454467 1103 124-125 1.2318975880438359 1104 1 0.6481481893749397 1104 2 0.7391257525679471 1104 3 0.647329212741429 1104 4 0.6799339000542446 1104 5 0.6564969981942603 1104 6 0.6781228228375937 1104 7 0.6901000372336306 1104 8 0.7691465968143056 1104 9 1.1105020163448067 1104 10-11 0.9497109052587405 1104 12-13 0.841293879886873 1104 14-15 0.848974763836253 1104 16-17 0.9763901924624605 1104 18-19 1.3177235653459007 1104 20-21 1.5373019050309438 1104 22-23 1.289715504640796 1104 24-25 1.6634253429739232 1104 26-27 1.7599606060725428 1104 28-29 1.5402831704639048 1104 30-31 1.5013017201721972 1104 32-33 1.4871500804985516 1104 34-35 1.8128962794589079 1104 36-37 1.8515389562488842 1104 38-39 1.926400675389516 1104 40-41 1.4899897598020608 1104 42-43 1.5906535136275082 1104 44-45 1.52661229447504 1104 46-47 1.3430226791929591 1104 48-49 1.28503517175794 1104 50-51 0.9860431418358822 1104 52-53 1.1970421084254106 1104 54-55 1.0389032746312452 1104 56-57 1.0706720621548733 1104 58-59 1.1001773876288858 1104 60-61 1.096548140700012 1104 62-63 1.0820446663933794 1104 64-65 1.1962915441338602 1104 66-67 1.1686128710505308 1104 68-69 1.1540490589139054 1104 70-71 1.1115894606898138 1104 72-73 0.984782479661586 1104 74-75 1.0367398618334427 1104 76-77 1.0251791348353834 1104 78-79 1.0750526368051752 1104 80-81 1.0927923459632147 1104 82-83 1.0187967678222911 1104 84-85 1.015845792044125 1104 86-87 1.1207146071159535 1104 88-89 1.1240081592952116 1104 90-91 1.1671234566351316 1104 92-93 1.2236008067929234 1104 94-95 1.2500669303245786 1104 96-97 1.0036113996525309 1104 98-99 1.143970412382867 1104 100-101 1.130616913741516 1104 102-103 1.1294950978549956 1104 104-105 1.2394754412568325 1104 106-107 1.2383185431342625 1104 108-109 1.287420306384714 1104 110-111 1.3560130187312964 1104 112-113 1.3055565894208314 1104 114-115 1.2415394709451704 1104 116-117 1.3141881481110005 1104 118-119 1.3342485922828864 1104 120-121 1.3096100003872095 1104 122-123 1.2820860899582271 1104 124-125 1.5777985620698125 1105 1 0.24793213911734568 1105 2 0.6381355443660084 1105 3 0.6383144958124447 1105 4 0.6855156412829082 1105 5 0.6968387810207872 1105 6 0.6768470279072893 1105 7 0.8304684830890778 1105 8 0.5835402476473242 1105 9 0.4327765410165263 1105 10-11 0.7543728827165239 1105 12-13 0.7385493152471803 1105 14-15 0.8622296553062085 1105 16-17 0.8494092888603859 1105 18-19 0.9315900898688056 1105 20-21 1.307597917800848 1105 22-23 1.5454601250868194 1105 24-25 1.6023384392159414 1105 26-27 1.3577425033817967 1105 28-29 1.14082338468938 1105 30-31 1.126659146490109 1105 32-33 1.193580277397487 1105 34-35 1.88008334762414 1105 36-37 1.8117684133152103 1105 38-39 1.8664993241674495 1105 40-41 1.35162502728274 1105 42-43 1.0217056668663957 1105 44-45 1.1776891353755907 1105 46-47 1.1300124276569612 1105 48-49 0.9932868255743799 1105 50-51 0.9189633680021458 1105 52-53 0.9511372307741688 1105 54-55 0.7469064573642292 1105 56-57 0.7232326430368623 1105 58-59 0.6974913532096636 1105 60-61 0.8028668356466149 1105 62-63 0.7744851883509654 1105 64-65 0.7819804069601055 1105 66-67 0.6563692480320995 1105 68-69 0.3812024528251676 1105 70-71 0.5346057224800127 1105 72-73 0.684973771392329 1105 74-75 0.6986647243549662 1105 76-77 0.7572608707491568 1105 78-79 0.7637704108585943 1105 80-81 0.7048038633287348 1105 82-83 0.6260135905104889 1105 84-85 0.6437858117446495 1105 86-87 0.5880395523725888 1105 88-89 0.7690017769987527 1105 90-91 0.6921396449026922 1105 92-93 0.5859503732751037 1105 94-95 0.6570130701203425 1105 96-97 0.7900387663728203 1105 98-99 0.6878344167623354 1105 100-101 0.7000610383083554 1105 102-103 0.6456717390928723 1105 104-105 0.772294440923762 1105 106-107 0.7544469074593039 1105 108-109 0.7264937351262226 1105 110-111 0.7586379319214309 1105 112-113 0.7158601891954817 1105 114-115 0.6860456025560069 1105 116-117 0.7274264526966867 1105 118-119 0.7736414680278543 1105 120-121 0.5570604036101585 1105 122-123 0.707229857718815 1105 124-125 0.906143244168554 1106 1 0.2701552984136022 1106 2 0.4797942446572847 1106 3 0.254858231325489 1106 4 0.4035417282277258 1106 5 0.23644639572425774 1106 6 0.2713248381686384 1106 7 0.06771940395123366 1106 8 0.37952021499826927 1106 9 0.5062180735034083 1106 10-11 0.623303110277476 1106 12-13 0.5138389309366751 1106 14-15 0.7566715551958936 1106 16-17 0.573229483992094 1106 18-19 0.5571031192103391 1106 20-21 0.878356938795747 1106 22-23 0.8444420066108549 1106 24-25 1.1224001911486141 1106 26-27 1.318251580563743 1106 28-29 1.2017817452564294 1106 30-31 0.9305797134657574 1106 32-33 0.8095927031487022 1106 34-35 1.1120130400061576 1106 36-37 1.0849902106640776 1106 38-39 1.160694819692445 1106 40-41 0.8549374843013204 1106 42-43 0.7340933095714313 1106 44-45 0.6859448759883158 1106 46-47 0.39940778537225796 1106 48-49 0.5685763627589999 1106 50-51 0.6294654358204852 1106 52-53 0.48136115807237445 1106 54-55 0.2928168357532286 1106 56-57 0.3688876675057067 1106 58-59 0.30394990420710144 1106 60-61 0.40259869664723524 1106 62-63 0.37029528237288645 1106 64-65 0.4083300739424338 1106 66-67 0.2953195077935078 1106 68-69 0.4197976350484325 1106 70-71 0.4090703192498282 1106 72-73 0.43337916920792097 1106 74-75 0.5299433421847262 1106 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 230558.0 3 37246.0 4 23012.0 5 18053.0 6 16447.0 7 17183.0 8 18074.0 9 18649.0 10 19698.0 11 20882.0 12 22282.0 13 24226.0 14 26985.0 15 24313.0 16 19205.0 17 17156.0 18 15652.0 19 14596.0 20 14304.0 21 15362.0 22 18526.0 23 24162.0 24 32522.0 25 41429.0 26 49687.0 27 55689.0 28 62948.0 29 73118.0 30 82651.0 31 93466.0 32 112402.0 33 139674.0 34 177128.0 35 239285.0 36 352564.0 37 451175.0 38 150849.0 39 416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60689236085425 18.995652806189923 13.2272041160315 23.170250716924322 2 14.944903954019455 20.27424079889357 41.05468181983833 23.726173427248646 3 19.039220193169186 25.893128735314484 28.665312574760414 26.402338496755917 4 11.894041685039154 16.690577962974054 38.18873373809337 33.22664661389342 5 13.35008931310106 38.7955181062014 33.681179293795 14.17321328690255 6 31.01979287424776 37.030574555132084 18.14008247361678 13.809550097003376 7 28.309258790837443 33.3530952545048 20.691344891952326 17.64630106270543 8 25.722712076242598 35.953432959033385 19.736186008383683 18.587668956340334 9 26.602682292324403 16.937120919643085 19.662458371068478 36.79773841696404 10-11 24.648340618002624 27.527985902595418 27.279426780594708 20.544246698807246 12-13 25.386895080942125 24.888582912480466 27.675836757977894 22.04868524859952 14-15 22.358656521728932 26.182928983571287 25.637290026259745 25.821124468440036 16-17 22.08632533959132 29.782832273983708 25.95731718210894 22.173525204316032 18-19 21.867329536075005 28.1357826256939 28.46273100584527 21.534156832385822 20-21 22.915047776029912 27.22659068352532 28.322748412171617 21.53561312827315 22-23 22.453749682259367 27.309692528093322 28.115549527578988 22.121008262068326 24-25 22.18964329229182 27.34427112116926 28.331454196051304 22.134631390487613 26-27 22.13964425633638 27.68787188955259 28.062648503203196 22.109835350907836 28-29 21.947447314447107 27.49787439318954 28.458325748860346 22.096352543503002 30-31 22.09090684403684 27.279882822824003 28.32190673056159 22.307303602577566 32-33 21.987670741538068 27.386773110323194 28.068602609466915 22.556953538671827 34-35 22.371824387033264 27.276225825215533 28.14095384220121 22.210995945549993 36-37 22.52529799627781 27.07398462949949 28.121971660470535 22.27874571375217 38-39 22.171286455149033 27.18688499511198 28.317289799458685 22.3245387502803 40-41 22.397406094911837 27.322386326274366 27.99867042153484 22.281537157278954 42-43 22.3571083061782 27.27319637198633 27.83375194428111 22.535943377554357 44-45 22.341878050175573 27.53317831384824 27.647431054551713 22.477512581424474 46-47 22.400430822965678 27.777346794446206 27.202104321219295 22.620118061368817 48-49 22.583842677898563 27.581732276189598 27.247875568044535 22.586549477867308 50-51 22.512551173499542 27.744299562612262 27.08583653201532 22.65731273187287 52-53 22.770292259921618 27.690258315311084 26.992892847169152 22.546556577598146 54-55 22.51454412062427 27.52170566330248 27.136454724218357 22.827295491854898 56-57 22.426537048910834 27.958534778603543 27.03817991499698 22.576748257488642 58-59 22.534045252715202 27.386874977111336 27.19884065647304 22.88023911370042 60-61 22.652913212517024 27.459574045842842 27.378117755306384 22.509394986333746 62-63 22.5116394057948 27.520644155394187 27.253407007463654 22.714309431347356 64-65 22.389363429336274 27.32360571026763 27.215054545336443 23.071976315059654 66-67 22.403591103856353 27.78209250498509 26.993065707191604 22.821250683966948 68-69 22.293789086846104 27.463601347281564 27.3341855193577 22.908424046514632 70-71 22.25612313729636 27.53726309325123 27.365693833483284 22.840919935969126 72-73 22.474266609651757 27.47422150292021 27.19075275917877 22.860759128249263 74-75 22.382840941645433 27.640773798570777 27.06317781881343 22.913207440970364 76-77 22.456604557883566 27.581048245382977 27.13662970064668 22.825717496086778 78-79 22.514834680716106 27.658552302577693 27.064858385897256 22.76175463080894 80-81 22.27530894354999 27.878923787850525 27.09195619205899 22.753811076540494 82-83 22.488808067031627 27.87033504087547 26.968666195807184 22.672190696285714 84-85 22.468676643668907 27.653690646542362 26.948712175825 22.928920533963733 86-87 22.52733473768683 27.346356438773146 27.178028867907784 22.948279955632245 88-89 22.50957085300177 27.350689370267915 27.191435284216936 22.94830449251338 90-91 22.353800958540244 27.739707241020906 27.23813373368749 22.668358066751356 92-93 22.520496698084955 27.487062598189667 27.110396756610868 22.882043947114514 94-95 22.369052637353438 27.45690065858046 27.128296150436988 23.045750553629123 96-97 22.47018012369125 27.459222937464194 27.119347170032615 22.95124976881194 98-99 22.308130829319335 27.380527141852035 27.01376486081795 23.29757716801068 100-101 22.151904215315636 27.379091898781038 27.295178181784063 23.17382570411926 102-103 22.423575917511133 27.146974968014565 27.267556269469985 23.16189284500432 104-105 22.197228032283938 27.156126230501314 27.437847573887808 23.20879816332694 106-107 22.142872992543765 27.291446537499365 27.393915405815505 23.17176506414136 108-109 22.220694810963014 27.37965863440774 26.984558232975196 23.41508832165405 110-111 22.070422187107564 27.324331048034217 27.356785747209468 23.248461017648754 112-113 22.09906717266073 27.270605078558248 27.375419166148912 23.25490858263211 114-115 21.789892674703975 27.50061878196288 27.23391112775629 23.475577415576854 116-117 21.84540264845896 27.438920988579053 27.339320544932228 23.376355818029754 118-119 21.859510155601114 27.492085724573833 27.119824330867587 23.528579788957465 120-121 21.789557770984203 27.426591325575767 27.372957522677556 23.410893380762477 122-123 21.839611715220304 27.420880698115944 27.29661917740605 23.4428884092577 124-125 21.782120332423407 27.428882928582667 27.111389558140175 23.67760718085375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1340.0 1 1402.5 2 1591.5 3 1482.5 4 1047.5 5 801.5 6 788.5 7 954.0 8 1299.0 9 1512.5 10 1438.0 11 1378.0 12 1492.5 13 1673.0 14 1860.0 15 2043.0 16 2248.5 17 2492.5 18 2760.5 19 3090.0 20 3575.0 21 4361.0 22 5479.0 23 6890.5 24 8656.5 25 11257.0 26 14968.5 27 19368.5 28 23925.0 29 28796.5 30 34127.5 31 40160.0 32 45988.0 33 51581.0 34 57742.5 35 63016.0 36 68681.0 37 74829.0 38 77932.5 39 79081.0 40 81238.0 41 86981.5 42 92442.0 43 95499.0 44 98942.5 45 101183.5 46 100918.0 47 100652.5 48 104260.0 49 104689.5 50 100971.5 51 97840.0 52 94694.0 53 93129.5 54 90175.5 55 83148.0 56 74261.5 57 67017.5 58 61733.0 59 55676.0 60 47963.0 61 39614.0 62 32034.5 63 25029.0 64 19464.0 65 15629.5 66 12290.5 67 9935.0 68 7807.0 69 5542.0 70 4076.0 71 3134.0 72 2586.0 73 2253.5 74 1757.5 75 1386.0 76 1029.0 77 655.0 78 430.0 79 291.0 80 189.0 81 129.0 82 99.0 83 77.0 84 52.5 85 37.0 86 33.0 87 32.0 88 22.0 89 16.0 90 13.5 91 13.0 92 12.0 93 10.0 94 8.5 95 7.5 96 5.0 97 4.5 98 3.0 99 2.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013025089714364474 2 0.0050873619105966505 3 0.007973808384694041 4 0.007937727803767823 5 0.015298166312716167 6 7.576921994505649E-4 7 0.009056225812480561 8 0.0 9 0.002309157179277912 10-11 0.0 12-13 0.0042755488397567595 14-15 0.02173855000804597 16-17 0.03346473880906662 18-19 0.006801189504591976 20-21 0.009290749588500975 22-23 0.0018942304986264125 24-25 0.012826646519270278 26-27 0.021287542746468255 28-29 4.510072615777172E-4 30-31 0.0016055858512166734 32-33 0.010102562659340866 34-35 0.010752013116012779 36-37 0.012862727100196496 38-39 1.8040290463108688E-5 40-41 0.0278181278941136 42-43 0.011022617472959408 44-45 0.018924264695801014 46-47 0.004834797844113128 48-49 0.027944409927355356 50-51 0.004329669711146085 52-53 0.0 54-55 0.018870143824411687 56-57 0.005159523072449085 58-59 1.9844319509419557E-4 60-61 0.005376006558006389 62-63 0.029369592873940944 64-65 0.01914074818135832 66-67 0.0021107139841837165 68-69 0.023849263992229686 70-71 0.0035539372212324114 72-73 0.013313734361774212 74-75 0.0 76-77 0.007216116185243475 78-79 0.0010102562659340866 80-81 0.004077105644662563 82-83 0.001677747013069108 84-85 0.0 86-87 0.006927471537833737 88-89 0.01914074818135832 90-91 0.02503992316279486 92-93 0.0054842483007850416 94-95 0.027637724989482512 96-97 0.020259246190071058 98-99 8.659339422292171E-4 100-101 0.009922159754709779 102-103 0.0 104-105 0.0014793038179749124 106-107 1.8040290463108688E-5 108-109 0.0 110-111 6.674907471350215E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 3.6080580926217375E-5 122-123 0.0 124-125 0.023452377602041295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2771574.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.84838575477086 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77151351746443 43.99261873410501 2 10.845425333787313 12.113989954318368 3 4.104777637521117 6.8773561481350916 4 1.9990673231615324 4.465787320547298 5 1.1393566566598778 3.181561503670344 6 0.7194814212393669 2.410912557406017 7 0.4688582730114665 1.832948438682204 8 0.32259926830708613 1.4413318704496778 9 0.23732540209921182 1.1928816545258598 >10 1.246070136478693 12.674375531864165 >50 0.092423528870111 3.5776000912634824 >100 0.05125540487247859 5.12234140675492 >500 0.001318640376584241 0.5020675365614062 >1k 5.27456150614554E-4 0.6142272517161838 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4356 0.15716701051460288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3091 0.11152507564293791 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.6080580926217375E-5 0.0 0.0 0.0 0.0 6 7.216116185243475E-5 0.0 0.0 0.0 0.0 7 7.216116185243475E-5 0.0 0.0 0.0 0.0 8 1.0824174277865213E-4 0.0 0.0 7.216116185243475E-5 0.0 9 1.0824174277865213E-4 0.0 0.0 7.216116185243475E-5 0.0 10-11 1.0824174277865213E-4 0.0 0.0 7.216116185243475E-5 0.0 12-13 1.0824174277865213E-4 0.0 0.0 1.0824174277865213E-4 0.0 14-15 1.0824174277865213E-4 0.0 0.0 1.0824174277865213E-4 0.0 16-17 1.0824174277865213E-4 0.0 0.0 3.427655187990651E-4 0.0 18-19 1.0824174277865213E-4 0.0 0.0 4.329669711146085E-4 0.0 20-21 1.0824174277865213E-4 0.0 0.0 5.77289294819478E-4 0.0 22-23 1.0824174277865213E-4 0.0 0.0 9.741756850078691E-4 0.0 24-25 1.804029046310869E-4 0.0 0.0 0.0014612635275118037 0.0 26-27 1.804029046310869E-4 0.0 0.0 0.0018401096272370862 0.0 28-29 2.3452377602041296E-4 0.0 0.0 0.0025797615362245425 0.0 30-31 2.5256406648352164E-4 1.8040290463108688E-5 0.0 0.004492032325314064 0.0 32-33 2.5256406648352164E-4 3.6080580926217375E-5 0.0 0.008136170998862019 0.0 34-35 2.88644647409739E-4 3.6080580926217375E-5 0.0 0.01333177465223732 0.0 36-37 3.247252283359564E-4 3.6080580926217375E-5 0.0 0.02321785382602088 0.0 38-39 3.9688639018839114E-4 3.6080580926217375E-5 0.0 0.04010356569949061 0.0 40-41 4.149266806514998E-4 3.6080580926217375E-5 0.0 0.061553471060126846 0.0 42-43 4.510072615777172E-4 3.6080580926217375E-5 0.0 0.0824441274164067 0.0 44-45 5.051281329670433E-4 3.6080580926217375E-5 0.0 0.10641967344187815 0.0 46-47 5.592490043563694E-4 3.6080580926217375E-5 0.0 0.13488725179266367 0.0 48-49 5.77289294819478E-4 3.6080580926217375E-5 0.0 0.1692540051248857 0.0 50-51 5.77289294819478E-4 3.6080580926217375E-5 0.0 0.2088885232723355 0.0 52-53 6.133698757456954E-4 3.6080580926217375E-5 0.0 0.24814419532006 0.0 54-55 6.133698757456954E-4 3.6080580926217375E-5 0.0 0.2897451051279887 0.0 56-57 7.216116185243476E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.33953630680616864 0.0 58-59 7.216116185243476E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.3983115731349767 0.0 60-61 7.396519089874563E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.467405885608683 0.0 62-63 7.937727803767823E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.5361935131445164 0.0 64-65 7.937727803767823E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.60550430910378 0.0 66-67 8.65933942229217E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.685332594403036 0.0 68-69 8.65933942229217E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.7807296503719547 0.0 70-71 8.839742326923257E-4 3.6080580926217375E-5 3.6080580926217375E-5 0.8953215753936211 0.0 72-73 9.020145231554345E-4 3.6080580926217375E-5 3.6080580926217375E-5 1.0159209171395027 0.0 74-75 9.200548136185432E-4 3.6080580926217375E-5 3.6080580926217375E-5 1.1352754788434298 0.0 76-77 9.380951040816518E-4 3.6080580926217375E-5 3.6080580926217375E-5 1.2670417603859756 0.0 78-79 9.380951040816518E-4 3.6080580926217375E-5 3.6080580926217375E-5 1.415982398449401 0.0 80-81 0.0012628203324176082 3.6080580926217375E-5 3.6080580926217375E-5 1.5918030693028582 0.0 82-83 0.0012628203324176082 3.6080580926217375E-5 3.6080580926217375E-5 1.775633629121936 0.0 84-85 0.001280860622880717 3.6080580926217375E-5 3.6080580926217375E-5 1.9624769174483525 0.0 86-87 0.0012989009133438257 3.6080580926217375E-5 3.6080580926217375E-5 2.1563017981839923 0.0 88-89 0.0012989009133438257 3.6080580926217375E-5 3.6080580926217375E-5 2.381300300839884 0.0 90-91 0.0013530217847331515 3.6080580926217375E-5 3.6080580926217375E-5 2.6336118032569216 0.0 92-93 0.001389102365659369 3.6080580926217375E-5 3.6080580926217375E-5 2.9092133206618334 0.0 94-95 0.0014071426561224776 3.6080580926217375E-5 3.6080580926217375E-5 3.1896676762013207 0.0 96-97 0.0014071426561224776 3.6080580926217375E-5 3.6080580926217375E-5 3.469995749707567 0.0 98-99 0.0014793038179749124 7.216116185243475E-5 3.6080580926217375E-5 3.7811727199057286 0.0 100-101 0.0015153843989011298 7.216116185243475E-5 3.6080580926217375E-5 4.145947393069786 0.0 102-103 0.0015514649798273473 7.216116185243475E-5 3.6080580926217375E-5 4.537439736409707 1.8040290463108688E-5 104-105 0.0018220693367739776 7.216116185243475E-5 3.6080580926217375E-5 4.924440047424316 3.6080580926217375E-5 106-107 0.001912270789089521 7.216116185243475E-5 3.6080580926217375E-5 5.314651530141356 3.6080580926217375E-5 108-109 0.001912270789089521 7.216116185243475E-5 3.6080580926217375E-5 5.727413375937283 3.6080580926217375E-5 110-111 0.002020512531868173 7.216116185243475E-5 3.6080580926217375E-5 6.192708547561782 3.6080580926217375E-5 112-113 0.0020565931127943904 7.216116185243475E-5 3.6080580926217375E-5 6.703357009410537 3.6080580926217375E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8195 0.0 54.5959 1 GGTATCA 4325 0.0 54.475372 1 ATCAACG 9710 0.0 44.79004 3 TCAACGC 10020 0.0 43.10744 4 CAACGCA 10075 0.0 42.931164 5 AACGCAG 10320 0.0 41.792896 6 TATCAAC 10520 0.0 41.567596 2 GTACATG 13625 0.0 37.335396 1 TACATGG 13895 0.0 35.581627 2 ACGCAGA 12335 0.0 34.676388 7 CGCAGAG 12215 0.0 34.3864 8 ACATGGG 14270 0.0 33.729343 3 GCAGAGT 13515 0.0 29.97935 9 CATGGGA 8775 0.0 29.018833 4 GAGTACT 5760 0.0 25.9346 12-13 CATGGGG 5990 0.0 25.427076 4 AGTACTT 6200 0.0 23.8541 12-13 AGAGTAC 12920 0.0 23.236364 10-11 CAGAGTA 13445 0.0 23.214228 10-11 ATGGGAG 4150 0.0 23.081345 5 >>END_MODULE