FastQCFastQC Report
Thu 26 May 2016
SRR1364581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771574
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83420.30098420608650533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73110.2637851271515752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47090.16990345558155764No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56650.061.457751
GTATCAA105000.056.2094841
ATCAACG135450.043.355653
TCAACGC140250.041.786894
CAACGCA141050.041.2954985
TATCAAC142100.040.8656462
AACGCAG144250.040.3794146
ACGCAGA170150.033.813317
CGCAGAG170550.033.5246938
GTACATG135000.033.4733471
TACATGG137000.032.4749032
ACATGGG142000.030.1150743
GCAGAGT188550.029.787819
GAGTACT107350.026.79660412-13
GTGGTAT31950.026.3094621
AGTACTT112400.025.8043912-13
CATGGGG60800.025.0774974
TGGTATC31700.024.98852
AGAGTAC179550.024.9178110-11
GTACTTT116550.024.40106214-15
Produced by FastQC (version 0.11.5)