##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364581_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2771574 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.232308428351544 31.0 30.0 34.0 26.0 34.0 2 30.087197383147625 31.0 30.0 34.0 26.0 34.0 3 30.355768238553257 31.0 30.0 34.0 26.0 34.0 4 33.934723012988286 35.0 35.0 37.0 30.0 37.0 5 33.40138130896018 35.0 35.0 37.0 27.0 37.0 6 33.45224735114415 36.0 35.0 37.0 28.0 37.0 7 33.24317445610328 36.0 33.0 37.0 26.0 37.0 8 33.174812940228186 36.0 33.0 37.0 26.0 37.0 9 34.408572529544585 38.0 34.0 39.0 26.0 39.0 10-11 34.35782158441377 38.0 34.0 39.0 26.0 39.0 12-13 34.25306666897582 38.0 34.0 39.0 25.5 39.0 14-15 35.23913270942793 39.0 34.0 41.0 25.5 41.0 16-17 35.25342152148923 39.0 34.0 41.0 25.0 41.0 18-19 35.280836088085685 39.0 34.0 41.0 25.0 41.0 20-21 35.3029397735727 39.0 34.0 41.0 25.0 41.0 22-23 35.26575097760334 39.0 34.0 41.0 25.0 41.0 24-25 35.23058179215132 39.0 34.0 41.0 25.0 41.0 26-27 35.11691443201589 39.0 34.0 41.0 25.0 41.0 28-29 34.96070951019168 39.0 34.0 41.0 24.5 41.0 30-31 34.798969827253394 38.5 34.0 41.0 24.0 41.0 32-33 34.598156498798154 38.0 33.5 41.0 24.0 41.0 34-35 34.4240070804532 38.0 33.0 40.0 24.0 41.0 36-37 34.22102422666687 38.0 33.0 40.0 22.5 41.0 38-39 34.05198021052297 38.0 33.0 40.0 21.5 41.0 40-41 34.07382086857504 38.0 33.0 40.0 21.0 41.0 42-43 33.94139449280445 38.0 33.0 40.0 19.5 41.0 44-45 33.77156644563703 38.0 33.0 40.0 18.0 41.0 46-47 33.517897591765546 38.0 33.0 40.0 15.0 41.0 48-49 33.23684285535944 38.0 32.0 40.0 10.5 41.0 50-51 33.06363874823476 38.0 32.0 40.0 9.0 41.0 52-53 32.8857690611905 38.0 32.0 40.0 8.0 41.0 54-55 32.6999042782188 38.0 31.5 40.0 7.0 41.0 56-57 32.49170399202764 37.0 31.0 40.0 6.0 41.0 58-59 32.289134441295815 37.0 31.0 40.0 2.0 41.0 60-61 32.06311359537938 36.5 31.0 40.0 2.0 41.0 62-63 31.896256784051232 36.0 31.0 40.0 2.0 41.0 64-65 31.771756770701415 36.0 31.0 39.5 2.0 41.0 66-67 31.472617725523477 35.5 31.0 39.0 2.0 41.0 68-69 31.191887894748618 35.0 30.0 39.0 2.0 41.0 70-71 30.8701209132428 35.0 30.0 38.5 2.0 40.0 72-73 30.583267486273144 35.0 30.0 37.5 2.0 40.0 74-75 30.23072106319369 35.0 30.0 37.0 2.0 39.5 76-77 29.917145095169747 35.0 29.0 36.5 2.0 39.0 78-79 29.61838435488282 35.0 29.0 36.0 2.0 39.0 80-81 29.33324168865778 34.0 29.0 36.0 2.0 37.5 82-83 29.04291676859431 34.0 29.0 35.0 2.0 37.0 84-85 28.813978988112893 34.0 29.0 35.0 2.0 37.0 86-87 28.58895017776902 34.0 29.0 35.0 2.0 36.0 88-89 28.4078058171999 34.0 29.0 35.0 2.0 36.0 90-91 28.26087378507664 34.0 29.0 35.0 2.0 36.0 92-93 28.14521676130603 34.0 29.0 35.0 2.0 35.0 94-95 28.030845108230917 34.0 28.0 35.0 2.0 35.0 96-97 27.913965133169818 34.0 28.0 35.0 2.0 35.0 98-99 27.824634485674927 34.0 28.0 35.0 2.0 35.0 100-101 27.723154604567657 34.0 27.0 35.0 2.0 35.0 102-103 26.43580579122188 32.0 24.5 34.0 2.0 35.0 104-105 27.386097574879834 33.5 27.0 35.0 2.0 35.0 106-107 27.551722775578064 34.0 27.0 35.0 2.0 35.0 108-109 27.553406656289894 34.0 27.0 35.0 2.0 35.0 110-111 27.474044351693298 34.0 27.0 35.0 2.0 35.0 112-113 27.391963555726818 34.0 27.0 35.0 2.0 35.0 114-115 27.28998486058824 34.0 27.0 35.0 2.0 35.0 116-117 27.164249087341705 34.0 27.0 35.0 2.0 35.0 118-119 27.031103986399064 34.0 27.0 35.0 2.0 35.0 120-121 26.839282660322258 34.0 25.5 35.0 2.0 35.0 122-123 26.544170027572783 34.0 25.0 35.0 2.0 35.0 124-125 24.721715169791608 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7850880207099422 1101 2 0.975991791143354 1101 3 0.7503412790901258 1101 4 0.6714994843767883 1101 5 0.7381508385081261 1101 6 0.777286919511937 1101 7 0.8703387104053775 1101 8 0.9875707450558551 1101 9 1.0332755133795928 1101 10-11 1.0374209431096446 1101 12-13 1.073844899654297 1101 14-15 1.217366184605254 1101 16-17 1.2279749216917821 1101 18-19 1.2368213011168265 1101 20-21 1.189176736948994 1101 22-23 1.2331413122553272 1101 24-25 1.2506996367789185 1101 26-27 1.3039603264186468 1101 28-29 1.3953829308962824 1101 30-31 1.44997125467345 1101 32-33 1.588469141273677 1101 34-35 1.5665669387796086 1101 36-37 1.48237831553773 1101 38-39 1.4313617142011097 1101 40-41 1.2841565810021223 1101 42-43 1.389919747810211 1101 44-45 1.3281875382150972 1101 46-47 1.3979804952137798 1101 48-49 1.5172047187262336 1101 50-51 1.504139962714305 1101 52-53 1.4747591403690024 1101 54-55 1.468873556920805 1101 56-57 1.4583761695701511 1101 58-59 1.5161622518215339 1101 60-61 1.4611486531497704 1101 62-63 1.5091498383523394 1101 64-65 1.4951286203357945 1101 66-67 1.5164382490479973 1101 68-69 1.6023586914283356 1101 70-71 1.515312091318151 1101 72-73 1.4101893151214107 1101 74-75 1.3882820881303424 1101 76-77 1.3953125174426546 1101 78-79 1.455359349207999 1101 80-81 1.2509274316918955 1101 82-83 1.379963038654541 1101 84-85 1.3534706505181937 1101 86-87 1.4158148897971756 1101 88-89 1.339178544220431 1101 90-91 1.391665216993438 1101 92-93 1.2998914183724253 1101 94-95 1.2601622990559989 1101 96-97 1.2913980292307947 1101 98-99 1.272839277960287 1101 100-101 1.250587446971931 1101 102-103 1.233102497901367 1101 104-105 1.3444751034216615 1101 106-107 1.249676246962153 1101 108-109 1.2000972583178537 1101 110-111 1.1586946335978539 1101 112-113 1.18724424806107 1101 114-115 1.2941229402610475 1101 116-117 1.2755481098266515 1101 118-119 1.383912037250159 1101 120-121 1.4211973057115195 1101 122-123 1.3468217542980625 1101 124-125 1.6646463830726042 1102 1 0.23297848968882207 1102 2 -0.4371536356319865 1102 3 0.21045531307960985 1102 4 0.1824130012967089 1102 5 0.39850709825054764 1102 6 0.11386267643685954 1102 7 0.22720698565918696 1102 8 0.3652105072373786 1102 9 0.6789744260003943 1102 10-11 0.7662434329466166 1102 12-13 0.7770408233049935 1102 14-15 0.9398313834243623 1102 16-17 0.949450316098762 1102 18-19 0.9584424989434268 1102 20-21 0.8576659463609531 1102 22-23 0.9622721719594409 1102 24-25 0.9166423501788046 1102 26-27 0.9217752517646716 1102 28-29 0.7041707387270293 1102 30-31 0.865344418008732 1102 32-33 1.0388055280973134 1102 34-35 1.1250811687568287 1102 36-37 0.7275747913206558 1102 38-39 0.24502040393650049 1102 40-41 -0.14339666824017883 1102 42-43 0.1860328731038976 1102 44-45 -0.19615752941236764 1102 46-47 0.07258340789464768 1102 48-49 -0.11396824446548237 1102 50-51 -0.12640045809706635 1102 52-53 -0.3657098297821548 1102 54-55 -0.3737407315698533 1102 56-57 -0.35266644012596515 1102 58-59 -0.28895602443630963 1102 60-61 -0.28658314329329215 1102 62-63 -0.331249111234424 1102 64-65 -0.34546354060882933 1102 66-67 -0.500687628544533 1102 68-69 -0.32244280201775766 1102 70-71 -0.773349693465871 1102 72-73 -0.49642053884992166 1102 74-75 -0.3751887945775323 1102 76-77 -0.8153024429660434 1102 78-79 -0.6012547217125324 1102 80-81 -0.6520921658592336 1102 82-83 -0.9182911269142373 1102 84-85 -0.7258604662539305 1102 86-87 -0.45869402769712053 1102 88-89 -0.5416486378674357 1102 90-91 -0.551706655588692 1102 92-93 -0.49577722475999764 1102 94-95 -0.2909374341499351 1102 96-97 -0.49690500929500914 1102 98-99 -0.7266523308346464 1102 100-101 -0.4797687726090665 1102 102-103 -0.4857466014962277 1102 104-105 -0.45448014086542443 1102 106-107 -0.35714902937898074 1102 108-109 -0.2713311210343541 1102 110-111 -0.1981675301353718 1102 112-113 -0.09536494865987066 1102 114-115 -0.2269937602721761 1102 116-117 -0.339006668518099 1102 118-119 -0.09889014382930483 1102 120-121 0.10082659484214318 1102 122-123 0.05774202881359969 1102 124-125 0.27456688266671136 1103 1 0.29976840371080726 1103 2 -0.09744833139163944 1103 3 0.23996119034932661 1103 4 0.3102737506736801 1103 5 0.3514759546827264 1103 6 0.4172395241142226 1103 7 0.5223527044073037 1103 8 0.5529728948361452 1103 9 0.7709151232451532 1103 10-11 0.7653170118538455 1103 12-13 0.7579359780547961 1103 14-15 0.8030030313383065 1103 16-17 0.7796659576520071 1103 18-19 0.8564328823442366 1103 20-21 0.9186261365207571 1103 22-23 0.8746554027637217 1103 24-25 0.8408960189716055 1103 26-27 0.833829557335477 1103 28-29 0.8797195082013474 1103 30-31 0.7601116292539913 1103 32-33 0.8731492477355545 1103 34-35 0.6886264544864602 1103 36-37 0.8742611905500084 1103 38-39 0.8644773718390582 1103 40-41 0.784290956379543 1103 42-43 0.7187431816118632 1103 44-45 0.7854661010250013 1103 46-47 0.808609412614409 1103 48-49 0.724483366190924 1103 50-51 0.7353661817715675 1103 52-53 0.8032713549271477 1103 54-55 0.8202751017014975 1103 56-57 0.8323868372030105 1103 58-59 0.9013149013472983 1103 60-61 0.8944770200684786 1103 62-63 0.7644109645320079 1103 64-65 0.839663165700042 1103 66-67 0.762687336187124 1103 68-69 0.7029098223599561 1103 70-71 0.7649071592169392 1103 72-73 0.7026805759247701 1103 74-75 0.8159348872129115 1103 76-77 0.862524248113985 1103 78-79 0.766613075429877 1103 80-81 0.7702290634977196 1103 82-83 0.8765042694901055 1103 84-85 0.9147369056496863 1103 86-87 1.0231087186883911 1103 88-89 0.9629552824373313 1103 90-91 0.9137497905195104 1103 92-93 0.8252092971264418 1103 94-95 0.7703788629517447 1103 96-97 0.9117026792622305 1103 98-99 0.9304678601279193 1103 100-101 0.8670638943149562 1103 102-103 0.6435076162431272 1103 104-105 0.7529450695936042 1103 106-107 0.8503781198648319 1103 108-109 0.8534475000355108 1103 110-111 0.8097269706006927 1103 112-113 0.7389111856230812 1103 114-115 0.8061019323271985 1103 116-117 0.8914435148772739 1103 118-119 0.7753464958150253 1103 120-121 0.7718959899502629 1103 122-123 0.8946953105105422 1103 124-125 1.2455570937100973 1104 1 0.4645069546335705 1104 2 0.56507302882709 1104 3 0.33518504544160166 1104 4 0.13898188464497707 1104 5 0.31157506609762464 1104 6 0.40110401488661296 1104 7 0.6109078992124992 1104 8 0.45545068020867063 1104 9 0.5686167637099473 1104 10-11 0.5252495135626631 1104 12-13 0.5044692145551366 1104 14-15 0.6489787661298294 1104 16-17 0.6887654108304133 1104 18-19 0.5222843006627613 1104 20-21 0.4338495644086606 1104 22-23 0.5098912195784919 1104 24-25 0.5812903286093416 1104 26-27 0.5015756270552316 1104 28-29 0.6218888520154309 1104 30-31 0.5426795547495473 1104 32-33 0.6718932668743065 1104 34-35 0.9389682179861225 1104 36-37 0.8985178549382553 1104 38-39 0.9211460321261384 1104 40-41 0.8344383418887702 1104 42-43 0.8813328945305159 1104 44-45 0.7547629226244581 1104 46-47 0.7327250995590404 1104 48-49 0.7306970538190853 1104 50-51 0.5487611749362955 1104 52-53 0.7127339318239336 1104 54-55 0.3787850128429895 1104 56-57 0.5240413997457338 1104 58-59 0.5639195664190666 1104 60-61 0.592150892248938 1104 62-63 0.587428736233985 1104 64-65 0.6470452572926568 1104 66-67 0.7827125412452283 1104 68-69 0.5638441183271468 1104 70-71 0.07314579558057943 1104 72-73 0.044159984673914465 1104 74-75 0.44476263840908103 1104 76-77 0.5664246240538375 1104 78-79 0.6086158095378735 1104 80-81 0.568026824946795 1104 82-83 0.5540354554094513 1104 84-85 0.6700363759162613 1104 86-87 0.6454237391942002 1104 88-89 0.5800306412890031 1104 90-91 0.6755064549077545 1104 92-93 0.6468429266548057 1104 94-95 0.686091439848532 1104 96-97 0.6690847708548482 1104 98-99 0.7689162538394712 1104 100-101 0.7456460029957448 1104 102-103 0.5866317618343793 1104 104-105 0.6120957872969548 1104 106-107 0.6790307172674304 1104 108-109 0.664307035237151 1104 110-111 0.695642042371027 1104 112-113 0.7186616982683312 1104 114-115 0.6752748646580251 1104 116-117 0.7080361327856792 1104 118-119 0.7343989565122193 1104 120-121 0.7238760822264076 1104 122-123 0.7925080241127276 1104 124-125 1.2029690896089384 1105 1 0.20895333802843652 1105 2 -0.009224032747678024 1105 3 0.20119889604360353 1105 4 0.1401476170721807 1105 5 -0.01744228170165485 1105 6 0.1931241955219889 1105 7 0.40152893519076116 1105 8 0.49374534476827847 1105 9 0.5584329925478357 1105 10-11 0.4901776651149703 1105 12-13 0.530325819722627 1105 14-15 0.5967559907654092 1105 16-17 0.4114189757039526 1105 18-19 0.47782801828621757 1105 20-21 0.5322373591192786 1105 22-23 0.5991769338642001 1105 24-25 0.5685597288417767 1105 26-27 0.588788091534056 1105 28-29 0.6362149151704912 1105 30-31 0.6826988788019008 1105 32-33 0.7861567472056734 1105 34-35 0.812557114827662 1105 36-37 0.9149537077654202 1105 38-39 0.8880079176363367 1105 40-41 0.6194742041055719 1105 42-43 0.6398542125522511 1105 44-45 0.7538268377137811 1105 46-47 0.6885895929083432 1105 48-49 0.7401231210488959 1105 50-51 0.5424367886288692 1105 52-53 0.6539616624369913 1105 54-55 0.624975122896636 1105 56-57 0.5336915781488543 1105 58-59 0.3283230276522602 1105 60-61 0.4771051266213817 1105 62-63 0.6297529728386912 1105 64-65 0.48064846890321533 1105 66-67 0.6553238306019828 1105 68-69 0.7523130891165124 1105 70-71 0.8134936240938977 1105 72-73 0.7279097765074098 1105 74-75 0.7200987165875645 1105 76-77 0.719961372971369 1105 78-79 0.7026468343194345 1105 80-81 0.6817553837967942 1105 82-83 0.6686648880272834 1105 84-85 0.657796739064942 1105 86-87 0.783961980709865 1105 88-89 0.7420286942678267 1105 90-91 0.6216666244172977 1105 92-93 0.6081345865302268 1105 94-95 0.5566884968170633 1105 96-97 0.6766363429991564 1105 98-99 0.5634851454369851 1105 100-101 0.6291543659743546 1105 102-103 0.3308058678699659 1105 104-105 0.5011248810019957 1105 106-107 0.5711843016288967 1105 108-109 0.6196845518373237 1105 110-111 0.5761870222807453 1105 112-113 0.514676239805965 1105 114-115 0.4954925270987651 1105 116-117 0.2515951953809008 1105 118-119 0.42066789523508774 1105 120-121 0.510673165946546 1105 122-123 0.4667655340515715 1105 124-125 1.0205884354523391 1106 1 -0.16758799299251592 1106 2 -0.5592547393677911 1106 3 -0.05043310810504309 1106 4 -0.1943681491735063 1106 5 0.07190983328690237 1106 6 0.22702058075392273 1106 7 0.257214549643912 1106 8 0.22266902950885736 1106 9 0.21355020929440371 1106 10-11 0.21693153125563214 1106 12-13 0.2466299583649274 1106 14-15 0.12170590465677833 1106 16-17 0.10154213190487127 1106 18-19 0.12264696586335333 1106 20-21 0.14892812304346137 1106 22-23 0.12291562972330894 1106 24-25 0.17193073928023495 1106 26-27 0.2203604073066714 1106 28-29 0.29070112096719924 1106 30-31 0.2691405377937812 1106 32-33 0.22661814725708496 1106 34-35 0.26067234741964995 1106 36-37 0.29342311845400815 1106 38-39 0.25250688159631807 1106 40-41 0.278718421997425 1106 42-43 0.18660184540604519 1106 44-45 0.11884494318037042 1106 46-47 -0.04658235070631633 1106 48-49 0.20547550026242334 1106 50-51 0.1769065510192931 1106 52-53 0.1285043266949799 1106 54-55 0.040015075147728396 1106 56-57 -0.3048379179261289 1106 58-59 0.024254478992027373 1106 60-61 0.10157639630536153 1106 62-63 -0.051895806679699064 1106 64-65 -0.020754805692025258 1106 66-67 -0.14859864292056457 1106 68-69 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-1.9089551114433192 2216 44-45 -1.7540587176634759 2216 46-47 -1.9250591298500979 2216 48-49 -1.8408665226449017 2216 50-51 -1.8251088955023569 2216 52-53 -1.8726954664434246 2216 54-55 -1.8220280929809292 2216 56-57 -1.6791583447639447 2216 58-59 -1.8332954674555744 2216 60-61 -1.586299121197925 2216 62-63 -1.609580625068812 2216 64-65 -1.6295008454070121 2216 66-67 -1.4524703431918944 2216 68-69 -1.2821237193606798 2216 70-71 -1.3761350477949819 2216 72-73 -1.260020307666526 2216 74-75 -1.273374027456498 2216 76-77 -1.3750067941034274 2216 78-79 -1.4743770607657396 2216 80-81 -1.3626329795301508 2216 82-83 -1.2796267180035343 2216 84-85 -1.3943409987418462 2216 86-87 -1.5193948784114575 2216 88-89 -1.3832361873121783 2216 90-91 -1.4333902159932386 2216 92-93 -1.413470528215246 2216 94-95 -1.322373644094199 2216 96-97 -1.4583299417256903 2216 98-99 -1.5749371010088353 2216 100-101 -1.5064387966174415 2216 102-103 -1.401595620262416 2216 104-105 -1.3327027100880606 2216 106-107 -1.3903382674088718 2216 108-109 -1.3742775827003868 2216 110-111 -1.3236245172929735 2216 112-113 -1.2956348879460649 2216 114-115 -1.4127477031770859 2216 116-117 -1.5117413360004406 2216 118-119 -1.5360892646113662 2216 120-121 -1.5219512786987366 2216 122-123 -1.4705339987526536 2216 124-125 -1.7982801093015794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75609.0 3 25325.0 4 12444.0 5 10179.0 6 10991.0 7 13057.0 8 14944.0 9 16407.0 10 18132.0 11 20078.0 12 22517.0 13 25607.0 14 30403.0 15 26166.0 16 18413.0 17 16004.0 18 14889.0 19 14132.0 20 13900.0 21 14338.0 22 16275.0 23 19923.0 24 25578.0 25 33995.0 26 42661.0 27 48670.0 28 55405.0 29 66347.0 30 76404.0 31 89073.0 32 109697.0 33 136332.0 34 176281.0 35 241581.0 36 387775.0 37 599138.0 38 232154.0 39 750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50976233781806 19.915638665647695 14.045053387672077 23.529545608862165 2 15.288574394396251 21.04692894612554 39.44355786407214 24.220938795406074 3 19.126568513054316 25.672776552240713 29.515069776235453 25.68558515846952 4 12.683014036538701 17.28879731440366 37.03616489908247 32.99202374997517 5 13.077009670317299 38.87191898899326 33.799566600061915 14.251504740627526 6 31.240948284260135 37.547545185515524 17.32535375205569 13.88615277816865 7 27.903828425350124 33.28808715842115 20.819915533257202 17.988168882971525 8 26.012772711904585 35.97031367439364 19.395556793608375 18.621356820093403 9 26.54145164654119 16.280273907555838 19.76383447485536 37.4144399710476 10-11 25.009209624770477 27.173859314457545 27.07045173525206 20.746479325519914 12-13 25.748762255671327 24.44876088460925 28.00686541293864 21.795611446780782 14-15 22.674736931926937 25.60828713364448 26.12173193314195 25.59524400128663 16-17 21.97866766345537 29.20153111939924 26.621239897862836 22.198561319282557 18-19 21.743766893985466 27.1070497091569 29.669947247197058 21.479236149660576 20-21 22.70348868675425 26.298470519893346 29.549364430477027 21.44867636287538 22-23 22.26918711172785 26.3314816779202 29.44280037264024 21.956530837711714 24-25 21.988732756188362 26.49234694797974 29.59322031452164 21.92569998131026 26-27 21.941232671398996 26.853351200437007 29.350398004888195 21.855018123275798 28-29 21.731622536508137 26.678360382944856 29.80328145667408 21.786735623872932 30-31 21.841560066590322 26.428213715383386 29.741764066194875 21.988462151831413 32-33 21.794420787610218 26.52907697936263 29.5212575958643 22.155244637162856 34-35 22.17855269244119 26.60035416698237 29.262704152947027 21.958388987629412 36-37 22.39500009741757 26.346635521909214 29.240731079162956 22.017633301510262 38-39 22.091129444856968 26.485275154118206 29.350560367502364 22.073035033522466 40-41 22.10467770299476 26.486682296774323 29.375690492117474 22.03294950811344 42-43 22.22354517685618 26.40315575193013 29.086612877736624 22.286686193477063 44-45 22.2336116589346 26.71316010324819 28.799772259373196 22.253455978444016 46-47 22.28248280579916 26.973643857245015 28.293471507526046 22.450401829429776 48-49 22.579209503336372 26.93575924727249 28.103994336792017 22.38103691259912 50-51 22.517407530415483 27.177918969134318 27.795907270725458 22.50876622972474 52-53 22.70078302076726 27.139217643115426 27.70440190303416 22.45559743308315 54-55 22.45797875142428 27.00018112451625 27.794386871864145 22.747453252195324 56-57 22.406041598595902 27.402596488784493 27.696788479278556 22.49457343334105 58-59 22.470510258313286 26.945449795908942 27.820607694519722 22.76343225125805 60-61 22.61256600040266 27.00149806572006 28.02995698473142 22.355978949145864 62-63 22.38428416488248 27.000036802192547 28.029000849336878 22.5866781835881 64-65 22.286121069373007 26.8098419378489 27.983888542514563 22.920148450263532 66-67 22.301172546718938 27.234091530660915 27.773442094636476 22.691293827983667 68-69 22.269367514632478 26.949848714124176 28.013450118957678 22.767333652285668 70-71 22.241008178024472 27.04865538499062 27.948685476195116 22.76165096078979 72-73 22.456517487896768 27.050513534908323 27.745551805580508 22.747417171614398 74-75 22.41275048402798 27.19735713146582 27.54736185809014 22.84253052641606 76-77 22.47381812645089 27.15433540652351 27.616726091383452 22.755120375642143 78-79 22.459367853789942 27.229148491074024 27.558636356092244 22.752847299043793 80-81 22.216762027642055 27.434446996544203 27.57429532821422 22.774495647599522 82-83 22.399636452066588 27.43971476135943 27.42723088035896 22.733417906215024 84-85 22.385330501729342 27.21628576397383 27.400711653378192 22.99767208091864 86-87 22.447785987312624 26.926612820007694 27.61230622021999 23.01329497245969 88-89 22.40482417456134 26.976630153291946 27.589971140608938 23.028574531537775 90-91 22.248170173338327 27.353410011783918 27.586562725729134 22.81185708914862 92-93 22.391842862640114 27.08179273295456 27.527867014082386 22.99849739032293 94-95 22.27284929069186 27.046075623454396 27.532261451435176 23.148813634418566 96-97 22.37552987364222 27.004931805408837 27.509965999680652 23.10957232126829 98-99 22.26265829921314 26.927486579690612 27.395706763872013 23.414148357224235 100-101 22.05931407914233 27.010260326491007 27.619857907296065 23.310567687070595 102-103 22.392203852395788 26.746372277990773 27.51974148985378 23.34168237975966 104-105 22.249180429604262 26.725301218729864 27.677666192567834 23.347852159098043 106-107 22.170535838959303 26.885795282954533 27.612489595071015 23.331179283015153 108-109 22.255873377366076 26.97788332550385 27.172483938729403 23.59375935840068 110-111 22.089589719609982 26.869717791517296 27.531328836239993 23.509363652632732 112-113 22.15353080956886 26.825496991961966 27.479024554278542 23.541947644190632 114-115 21.830158603017637 27.01446903456303 27.418318976870182 23.73705338554915 116-117 21.87224660066807 26.944400546404317 27.50637363462062 23.676979218306997 118-119 21.859636437634357 27.03074137656076 27.24937165668317 23.860250529121718 120-121 21.80759863614223 26.958741498439505 27.469574786671718 23.76408507874655 122-123 21.87151183192025 26.967144129052862 27.31281498941428 23.84852904961261 124-125 21.777724488530062 27.013471325198257 27.17302607427241 24.035778111999274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 428.0 1 327.0 2 420.5 3 645.5 4 481.5 5 366.5 6 790.5 7 1811.0 8 2963.5 9 3438.0 10 2806.5 11 1945.5 12 1712.5 13 1775.0 14 2024.5 15 2352.0 16 2695.0 17 2982.0 18 3435.0 19 3937.0 20 4414.5 21 5201.5 22 6290.5 23 7672.0 24 9276.0 25 11836.0 26 15620.5 27 19842.0 28 24334.5 29 29400.5 30 34862.5 31 40990.0 32 46308.5 33 51249.0 34 57351.0 35 62665.5 36 68060.5 37 74156.0 38 77689.0 39 78904.0 40 80606.0 41 85972.0 42 91280.0 43 94699.5 44 98073.5 45 100396.5 46 100711.0 47 100215.0 48 104484.5 49 105662.0 50 101714.5 51 97957.0 52 93991.5 53 93180.0 54 91288.0 55 83632.0 56 74371.5 57 67009.5 58 61845.5 59 55893.5 60 47931.0 61 39236.5 62 31362.0 63 24279.0 64 18728.0 65 14885.0 66 11499.0 67 9340.5 68 7158.0 69 4868.5 70 3542.0 71 2810.0 72 2333.0 73 2099.5 74 1721.5 75 1361.5 76 925.5 77 496.0 78 271.0 79 164.0 80 112.5 81 85.5 82 51.0 83 21.5 84 15.0 85 11.0 86 9.5 87 6.5 88 3.5 89 4.0 90 3.5 91 2.5 92 2.0 93 1.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09994320916562215 2 3.6080580926217375E-5 3 0.0 4 0.09846390534764722 5 0.0 6 0.0 7 0.002489560083908999 8 0.007107874442464823 9 0.004618314358555824 10-11 6.133698757456954E-4 12-13 0.0 14-15 1.8040290463108688E-5 16-17 2.5256406648352164E-4 18-19 0.002255036307888586 20-21 0.0015875455607535646 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.8040290463108688E-5 52-53 0.0 54-55 0.0 56-57 0.009182507845722323 58-59 6.133698757456954E-4 60-61 0.0 62-63 0.0 64-65 2.1648348555730428E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0014432232370486951 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0011726188801020646 90-91 0.0 92-93 0.002255036307888586 94-95 0.0 96-97 0.009976280626099105 98-99 0.004383790582535411 100-101 0.0026519226980769774 102-103 0.0 104-105 0.0 106-107 0.0018401096272370862 108-109 0.0 110-111 2.1648348555730428E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.659339422292171E-4 122-123 0.005466208010321933 124-125 0.0944770011553002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2771574.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.41716445695975 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66291867953606 35.6654964808675 2 12.979448012857087 12.568561379981222 3 5.243864428441823 7.6167913926560695 4 2.505187893852743 4.851763770090116 5 1.4403302970322605 3.4868354431876325 6 0.9070668125324285 2.635056182149973 7 0.6198387812710436 2.1007585346721136 8 0.4471684591019726 1.7320503059444392 9 0.32254118033456625 1.4054876435160546 >10 1.6598727912023443 14.641693523627735 >50 0.13194571933960164 4.416422852753814 >100 0.07644607595616239 6.815771993863012 >500 0.0023970620742770764 0.7870610140450138 >1k 8.239900880195421E-4 0.7085398815812333 >5k 1.4981637963991674E-4 0.5677096010642426 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8342 0.30098420608650533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7311 0.2637851271515752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4709 0.16990345558155764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6080580926217375E-5 0.0 0.0 0.0 0.0 3 3.6080580926217375E-5 0.0 0.0 0.0 0.0 4 3.6080580926217375E-5 0.0 0.0 0.0 0.0 5 3.6080580926217375E-5 0.0 0.0 0.0 0.0 6 3.6080580926217375E-5 0.0 0.0 0.0 0.0 7 1.0824174277865213E-4 0.0 0.0 0.0 0.0 8 1.0824174277865213E-4 0.0 0.0 3.6080580926217375E-5 0.0 9 1.0824174277865213E-4 0.0 0.0 3.6080580926217375E-5 0.0 10-11 1.0824174277865213E-4 0.0 0.0 3.6080580926217375E-5 0.0 12-13 1.443223237048695E-4 0.0 0.0 1.0824174277865213E-4 0.0 14-15 2.88644647409739E-4 0.0 0.0 1.0824174277865213E-4 0.0 16-17 3.608058092621738E-4 0.0 0.0 3.427655187990651E-4 0.0 18-19 4.3296697111460856E-4 0.0 0.0 4.690475520408259E-4 0.0 20-21 4.690475520408259E-4 0.0 0.0 6.133698757456955E-4 0.0 22-23 4.690475520408259E-4 0.0 0.0 9.922159754709777E-4 0.0 24-25 5.412087138932606E-4 0.0 0.0 0.0014432232370486951 0.0 26-27 5.412087138932606E-4 0.0 0.0 0.0018761902081633037 0.0 28-29 6.133698757456954E-4 0.0 0.0 0.0026338824076138686 0.0 30-31 6.133698757456954E-4 0.0 0.0 0.004654394939482042 0.0 32-33 6.133698757456954E-4 0.0 0.0 0.008641299131829061 0.0 34-35 6.314101662088041E-4 0.0 0.0 0.014414192080023843 0.0 36-37 6.494504566719128E-4 0.0 0.0 0.025364648391130815 0.0 38-39 7.216116185243476E-4 0.0 0.0 0.04311629420682976 0.0 40-41 7.216116185243476E-4 0.0 0.0 0.0654321335096952 0.0 42-43 7.216116185243476E-4 0.0 0.0 0.0865933942229217 0.0 44-45 7.216116185243476E-4 0.0 0.0 0.11083954460533979 0.0 46-47 7.576921994505649E-4 0.0 0.0 0.13966792876538747 0.0 48-49 7.576921994505649E-4 0.0 0.0 0.1742150850022406 0.0 50-51 7.576921994505649E-4 0.0 0.0 0.21359703908320687 0.0 52-53 7.576921994505649E-4 0.0 0.0 0.25319547664973047 0.0 54-55 7.576921994505649E-4 0.0 0.0 0.2945077418102493 0.0 56-57 7.576921994505649E-4 0.0 0.0 0.3442989434884293 0.0 58-59 7.576921994505649E-4 0.0 0.0 0.40276752487936457 0.0 60-61 7.576921994505649E-4 0.0 0.0 0.4723489251955748 0.0 62-63 7.757324899136735E-4 0.0 0.0 0.541425197378818 0.0 64-65 7.937727803767823E-4 0.0 0.0 0.6111509200187331 0.0 66-67 8.298533613029996E-4 0.0 0.0 0.6912498096749355 0.0 68-69 8.298533613029996E-4 0.0 0.0 0.7853840453114368 0.0 70-71 8.478936517661083E-4 0.0 0.0 0.8996692853952303 0.0 72-73 8.839742326923257E-4 0.0 0.0 1.0198176198795341 0.0 74-75 9.020145231554345E-4 0.0 0.0 1.1389737383883671 0.0 76-77 9.020145231554345E-4 0.0 0.0 1.2705054961548925 0.0 78-79 9.020145231554345E-4 0.0 0.0 1.42114192152185 0.0 80-81 0.001046336846860304 0.0 0.0 1.5985140573551346 0.0 82-83 0.0011184980087127388 0.0 0.0 1.783282712278294 0.0 84-85 0.0011184980087127388 0.0 0.0 1.9706311287376774 0.0 86-87 0.0011184980087127388 0.0 0.0 2.1661878773577756 0.0 88-89 0.0011184980087127388 0.0 0.0 2.39363985951665 0.0 90-91 0.001208699461028282 0.0 0.0 2.6484048414366708 0.0 92-93 0.0012989009133438257 0.0 0.0 2.926026871373451 0.0 94-95 0.0012989009133438257 0.0 0.0 3.208140933635544 0.0 96-97 0.0012989009133438257 0.0 0.0 3.4887576517892 0.0 98-99 0.0014071426561224776 0.0 0.0 3.802279859747566 0.0 100-101 0.0014251829465855865 0.0 0.0 4.1707167118756345 0.0 102-103 0.0014612635275118037 0.0 0.0 4.565384146337063 0.0 104-105 0.0016957873035322168 0.0 0.0 4.956028596025218 0.0 106-107 0.0017499081749215429 0.0 0.0 5.3505336678724795 0.0 108-109 0.001804029046310869 0.0 0.0 5.768545238193171 0.0 110-111 0.0018220693367739776 0.0 0.0 6.241850298783291 0.0 112-113 0.0018401096272370862 0.0 0.0 6.760905535987854 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5665 0.0 61.45775 1 GTATCAA 10500 0.0 56.209484 1 ATCAACG 13545 0.0 43.35565 3 TCAACGC 14025 0.0 41.78689 4 CAACGCA 14105 0.0 41.295498 5 TATCAAC 14210 0.0 40.865646 2 AACGCAG 14425 0.0 40.379414 6 ACGCAGA 17015 0.0 33.81331 7 CGCAGAG 17055 0.0 33.524693 8 GTACATG 13500 0.0 33.473347 1 TACATGG 13700 0.0 32.474903 2 ACATGGG 14200 0.0 30.115074 3 GCAGAGT 18855 0.0 29.78781 9 GAGTACT 10735 0.0 26.796604 12-13 GTGGTAT 3195 0.0 26.309462 1 AGTACTT 11240 0.0 25.80439 12-13 CATGGGG 6080 0.0 25.077497 4 TGGTATC 3170 0.0 24.9885 2 AGAGTAC 17955 0.0 24.91781 10-11 GTACTTT 11655 0.0 24.401062 14-15 >>END_MODULE