FastQCFastQC Report
Thu 26 May 2016
SRR1364580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789914
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42610.15272872210397884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.1158100213841717No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30650.10986001719049406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76100.057.94071
GGTATCA43050.057.7767751
ATCAACG95300.046.2649543
CAACGCA95700.046.2581065
TCAACGC97200.045.4830324
AACGCAG100300.043.771246
TATCAAC101150.043.6480522
GTACATG136200.036.8299141
ACGCAGA118600.036.7163777
CGCAGAG118600.035.967868
TACATGG139050.035.55962
ACATGGG138000.034.622893
GCAGAGT133750.030.8267239
CATGGGA76650.028.256354
CATGGGG66100.027.6352714
AGAGTAC122450.025.48966810-11
CAGAGTA128250.024.87052210-11
GTATACG1951.4650795E-624.4121251
GAGTACT67150.023.75163512-13
TAAGGTG16600.023.2987235
Produced by FastQC (version 0.11.5)