##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364580_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2789914 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.667321645039955 31.0 29.0 33.0 22.0 34.0 2 28.67130778941573 31.0 28.0 34.0 16.0 34.0 3 28.8648517481184 31.0 28.0 34.0 22.0 34.0 4 32.2287289142246 35.0 32.0 37.0 22.0 37.0 5 32.35617047693943 35.0 33.0 37.0 23.0 37.0 6 32.35432633407338 35.0 33.0 37.0 23.0 37.0 7 32.16433087184766 35.0 32.0 37.0 22.0 37.0 8 32.03570683540783 35.0 32.0 37.0 19.0 37.0 9 33.18067438637893 37.0 33.0 39.0 17.0 39.0 10-11 33.10092640848428 37.0 33.0 39.0 17.0 39.0 12-13 33.01783800504245 37.0 33.0 39.0 17.0 39.0 14-15 33.762415257244484 38.0 32.5 41.0 16.0 41.0 16-17 33.877585832394836 38.0 33.0 41.0 17.0 41.0 18-19 33.694766218600286 38.5 32.0 41.0 16.0 41.0 20-21 33.414670846484874 38.0 32.0 41.0 11.5 41.0 22-23 33.3282214075416 38.0 32.0 40.5 12.0 41.0 24-25 33.15297084426258 38.0 32.0 40.5 10.0 41.0 26-27 33.05976976351242 38.0 31.5 40.0 10.5 41.0 28-29 33.127496940765916 38.0 32.0 40.0 10.0 41.0 30-31 33.06327345574093 38.0 31.5 40.0 10.0 41.0 32-33 33.03221443384993 38.0 32.0 40.0 9.0 41.0 34-35 32.55362548809748 38.0 30.5 40.0 8.5 41.0 36-37 32.41133490136255 38.0 30.5 40.0 8.0 41.0 38-39 32.20478193951498 38.0 30.0 40.0 8.0 41.0 40-41 32.397346656563606 38.0 30.5 40.0 7.0 41.0 42-43 32.27381973064403 38.0 30.5 40.0 2.0 41.0 44-45 32.12295486527542 37.5 30.0 40.0 2.0 41.0 46-47 32.06766785642855 37.0 30.0 40.0 2.0 41.0 48-49 31.893495104150162 37.0 30.0 40.0 2.0 41.0 50-51 31.714620235605828 37.0 30.0 40.0 2.0 41.0 52-53 31.631512476728673 37.0 30.0 40.0 2.0 41.0 54-55 31.645009666964647 37.0 30.0 40.0 2.0 41.0 56-57 31.505568451213907 37.0 30.0 40.0 2.0 41.0 58-59 31.29235435214132 36.0 30.0 40.0 2.0 41.0 60-61 31.07482237803746 36.0 30.0 40.0 2.0 41.0 62-63 30.782230204945385 35.5 29.0 39.5 2.0 41.0 64-65 30.49477188185729 35.0 29.0 39.0 2.0 41.0 66-67 30.24063071478189 35.0 29.0 39.0 2.0 40.5 68-69 30.192923509470184 35.0 29.0 39.0 2.0 40.0 70-71 29.948593397502577 35.0 29.0 38.0 2.0 40.0 72-73 29.677161374866753 35.0 29.0 37.0 2.0 40.0 74-75 29.383739606310446 35.0 29.0 37.0 2.0 39.0 76-77 29.08643994044261 34.5 29.0 36.0 2.0 39.0 78-79 28.785760062854983 34.0 29.0 36.0 2.0 38.5 80-81 28.50461161168409 34.0 28.0 35.5 2.0 37.0 82-83 28.259786143945654 34.0 28.0 35.0 2.0 37.0 84-85 28.021560879654352 34.0 28.0 35.0 2.0 36.5 86-87 27.83469777204602 34.0 27.5 35.0 2.0 36.0 88-89 27.64006094811525 34.0 27.0 35.0 2.0 36.0 90-91 27.510001921206175 34.0 27.0 35.0 2.0 36.0 92-93 27.388098522033296 34.0 27.0 35.0 2.0 35.0 94-95 27.261203571149505 34.0 27.0 35.0 2.0 35.0 96-97 27.151919198943048 34.0 26.5 35.0 2.0 35.0 98-99 27.053880872313627 34.0 26.0 35.0 2.0 35.0 100-101 26.96130723025871 34.0 26.0 35.0 2.0 35.0 102-103 25.789810366914537 31.5 24.0 34.0 2.0 35.0 104-105 26.67077372277425 33.5 25.0 35.0 2.0 35.0 106-107 26.815236957124846 34.0 25.0 35.0 2.0 35.0 108-109 26.770566404555836 34.0 25.0 35.0 2.0 35.0 110-111 26.677391310269776 34.0 25.0 35.0 2.0 35.0 112-113 26.583212242384533 34.0 25.0 35.0 2.0 35.0 114-115 26.457580412872943 34.0 25.0 35.0 2.0 35.0 116-117 26.344650587795897 34.0 25.0 35.0 2.0 35.0 118-119 26.19043167638859 34.0 24.5 35.0 2.0 35.0 120-121 26.013199510809294 34.0 24.0 35.0 2.0 35.0 122-123 25.7771253880944 34.0 23.0 35.0 2.0 35.0 124-125 24.344549330194408 32.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7358703597649736 1101 2 0.7498989679529551 1101 3 0.6746406612869045 1101 4 0.7751116320414937 1101 5 0.7601019721033495 1101 6 0.803285809146864 1101 7 0.7988634770397596 1101 8 0.7476314596829994 1101 9 1.0490585193066622 1101 10-11 1.1455263947739311 1101 12-13 0.9059253557006812 1101 14-15 1.3267084763863366 1101 16-17 1.0557647520402327 1101 18-19 1.4222279929410035 1101 20-21 1.7303529835495794 1101 22-23 1.6302506071726341 1101 24-25 1.8326487866403554 1101 26-27 1.9090133281608175 1101 28-29 1.6391557909813699 1101 30-31 1.7325311867012516 1101 32-33 1.6940880343406945 1101 34-35 2.011232637330629 1101 36-37 1.952610485625506 1101 38-39 1.7728057654595446 1101 40-41 1.486624217964085 1101 42-43 1.534418465092351 1101 44-45 1.4563257044359261 1101 46-47 1.2932547466264808 1101 48-49 1.351935645498365 1101 50-51 1.3077875418290965 1101 52-53 1.2366465007033902 1101 54-55 1.1105997663775788 1101 56-57 1.0930828309737919 1101 58-59 1.1348906986757754 1101 60-61 1.0551275967700668 1101 62-63 0.9597068846452537 1101 64-65 1.0840545134017923 1101 66-67 1.047877353867598 1101 68-69 0.8762848523627369 1101 70-71 0.8223997612461851 1101 72-73 0.7038842627195656 1101 74-75 0.665095539838152 1101 76-77 0.9257727907736779 1101 78-79 0.911564118122115 1101 80-81 0.9828219899781878 1101 82-83 0.8649520230291685 1101 84-85 0.9414729228666872 1101 86-87 0.9473450959981875 1101 88-89 0.8397349936754637 1101 90-91 0.8783400029871906 1101 92-93 0.744105580129375 1101 94-95 0.7682589122239278 1101 96-97 0.7232242010501224 1101 98-99 0.8636173186892151 1101 100-101 0.9082168384278937 1101 102-103 0.8258665421216485 1101 104-105 0.9242715863811668 1101 106-107 0.8103093509763575 1101 108-109 0.8917435220030541 1101 110-111 0.6842623923975708 1101 112-113 0.7786184689563314 1101 114-115 0.9030022220467941 1101 116-117 1.014710671328487 1101 118-119 0.9302434599234743 1101 120-121 1.0501465269287849 1101 122-123 1.1555229253469932 1101 124-125 1.4949111370654755 1102 1 0.2130270831938077 1102 2 0.4206917188079444 1102 3 0.5893887883299911 1102 4 0.5634459640059504 1102 5 0.6503604113476129 1102 6 -0.08899532507923169 1102 7 -0.6245100268106363 1102 8 0.21087598559773113 1102 9 0.6730749204785766 1102 10-11 0.5973891578400199 1102 12-13 0.640811121771506 1102 14-15 0.9173962006043084 1102 16-17 0.8208251300740841 1102 18-19 0.9934016292152137 1102 20-21 1.2942036047856789 1102 22-23 1.1608092572331614 1102 24-25 1.31080485813834 1102 26-27 0.8818553255483366 1102 28-29 1.3023956133491694 1102 30-31 1.1754736815100841 1102 32-33 1.02380392878608 1102 34-35 1.5258427540431185 1102 36-37 1.6001751010966743 1102 38-39 1.5851626001886814 1102 40-41 1.3655026772926604 1102 42-43 1.2728264859586673 1102 44-45 1.3571326825298513 1102 46-47 0.9644797313696571 1102 48-49 0.7818535037962846 1102 50-51 1.2072094357250016 1102 52-53 0.9830173831329354 1102 54-55 0.8052258593013271 1102 56-57 0.8216741928164666 1102 58-59 0.999344551220311 1102 60-61 1.0042919049654735 1102 62-63 0.8671441375746127 1102 64-65 0.9218288685772045 1102 66-67 0.9195375100932779 1102 68-69 0.7912581100665079 1102 70-71 0.7590828878743849 1102 72-73 0.8787299597778606 1102 74-75 0.5376360687341766 1102 76-77 0.7515353285562902 1102 78-79 0.6191247669919804 1102 80-81 0.2958876962081156 1102 82-83 0.4651521473477054 1102 84-85 0.13788285435791536 1102 86-87 0.6630176369855505 1102 88-89 0.8665007793393009 1102 90-91 0.24630379959970483 1102 92-93 0.6720498731534441 1102 94-95 0.557662692653075 1102 96-97 0.6629998956326872 1102 98-99 0.7623598302293217 1102 100-101 0.758777681212937 1102 102-103 0.6408770885945607 1102 104-105 0.9070315020637203 1102 106-107 0.879199984057923 1102 108-109 0.39728784916887605 1102 110-111 0.6724636213421249 1102 112-113 0.38098564922466593 1102 114-115 0.4199867526413037 1102 116-117 0.35548632527150303 1102 118-119 0.6420314428195439 1102 120-121 0.9413056586189299 1102 122-123 0.890109073780522 1102 124-125 1.1758791428713842 1103 1 0.5229120206540685 1103 2 0.4947031139698588 1103 3 0.37639450004479613 1103 4 0.4584595587906293 1103 5 0.4245282343045247 1103 6 0.5054695314709505 1103 7 0.5592673327182638 1103 8 0.6471890567006611 1103 9 0.7769025196242794 1103 10-11 0.849225187318261 1103 12-13 0.7799054461658557 1103 14-15 1.0707280040897444 1103 16-17 0.9172169956930603 1103 18-19 1.0081125074683257 1103 20-21 1.4150147486816707 1103 22-23 1.337047847470977 1103 24-25 1.4126459164403045 1103 26-27 1.473963051715522 1103 28-29 1.4070786496144265 1103 30-31 1.2719423787360569 1103 32-33 1.258852336938645 1103 34-35 1.609460304960173 1103 36-37 1.5628911080150303 1103 38-39 1.6441436072910562 1103 40-41 1.4492645348152067 1103 42-43 1.2574080671893242 1103 44-45 1.0605812231335463 1103 46-47 1.1274123197473571 1103 48-49 0.9487920491958874 1103 50-51 0.9971163179656735 1103 52-53 1.0374981442215194 1103 54-55 0.9245631818854712 1103 56-57 0.8095927194270942 1103 58-59 0.7552166710880286 1103 60-61 0.628480897602472 1103 62-63 0.7008724272041036 1103 64-65 0.8877975548635781 1103 66-67 0.8344496713268086 1103 68-69 0.9302204525723141 1103 70-71 0.8860694499189066 1103 72-73 0.874478950069772 1103 74-75 0.9091496790943552 1103 76-77 0.8462757103726304 1103 78-79 0.9854219126384152 1103 80-81 0.933520863219961 1103 82-83 0.9173313693109364 1103 84-85 0.8545068848535635 1103 86-87 0.7908819958998521 1103 88-89 0.8131674889728693 1103 90-91 0.7546910521562786 1103 92-93 0.8308000857972111 1103 94-95 0.7486011932766345 1103 96-97 1.0046134317449287 1103 98-99 0.9054478717317309 1103 100-101 0.9226006455866482 1103 102-103 0.8347446882309946 1103 104-105 0.8747158963657888 1103 106-107 0.8812399508535513 1103 108-109 1.000788956386394 1103 110-111 1.0577548235916865 1103 112-113 1.1237945019670796 1103 114-115 1.1966867961113685 1103 116-117 1.0900089259473376 1103 118-119 1.159386538210871 1103 120-121 1.0500641056435391 1103 122-123 1.0205908237863 1103 124-125 1.0647850206476193 1104 1 0.7126255487072939 1104 2 0.802311276349208 1104 3 0.6701345339287315 1104 4 0.7727866889104362 1104 5 0.6874236412275962 1104 6 0.6814307807875366 1104 7 0.8325597250438932 1104 8 0.791835331157543 1104 9 0.9811544497772715 1104 10-11 0.9293932203914537 1104 12-13 0.9823162722197196 1104 14-15 1.0068762478599211 1104 16-17 1.0961160760719508 1104 18-19 1.2669398851215163 1104 20-21 1.4638608845538101 1104 22-23 1.3298936209413128 1104 24-25 1.7502628264312463 1104 26-27 1.7801490840432237 1104 28-29 1.6633055608874585 1104 30-31 1.6220994681445404 1104 32-33 1.6192116048636507 1104 34-35 1.8205756437387635 1104 36-37 1.9251979143528004 1104 38-39 1.955362738754161 1104 40-41 1.6143233351983781 1104 42-43 1.5682235280185992 1104 44-45 1.5327195418045108 1104 46-47 1.4577674814773829 1104 48-49 1.4474176063846897 1104 50-51 1.2998824749623559 1104 52-53 1.4070826387709907 1104 54-55 1.2443409529796092 1104 56-57 1.2418624628596575 1104 58-59 1.238370821869772 1104 60-61 1.1321066661746535 1104 62-63 1.1900079052750563 1104 64-65 1.2058120650458655 1104 66-67 1.1958850583252065 1104 68-69 1.2002087928033234 1104 70-71 1.1391462449947376 1104 72-73 1.0388514573306722 1104 74-75 1.0877740406542387 1104 76-77 1.0293996733806985 1104 78-79 1.1010845294966884 1104 80-81 1.010263583874103 1104 82-83 1.03193547795658 1104 84-85 0.9961717784990824 1104 86-87 1.0250339849484433 1104 88-89 1.008512567158995 1104 90-91 1.0322438671011973 1104 92-93 1.042371344299557 1104 94-95 1.0099064508665023 1104 96-97 0.8929301798379541 1104 98-99 0.939753218489848 1104 100-101 1.0102783846576635 1104 102-103 1.0515165347026887 1104 104-105 1.0621624239310563 1104 106-107 1.0548500176459257 1104 108-109 1.1167312252264736 1104 110-111 1.109345097580082 1104 112-113 1.077594450969844 1104 114-115 1.0708948046715214 1104 116-117 1.0697494076351468 1104 118-119 1.0891960921346637 1104 120-121 1.1158311225488475 1104 122-123 1.0964383875703732 1104 124-125 1.408410469890331 1105 1 0.23729086456736326 1105 2 0.4867389195725558 1105 3 0.5167055089603743 1105 4 0.46711755280100675 1105 5 0.43069428571948976 1105 6 0.5260680434700404 1105 7 0.5027909270879221 1105 8 0.36689084424705953 1105 9 0.3414207008209118 1105 10-11 0.6375334070713095 1105 12-13 0.5240298340493581 1105 14-15 0.6253001129263822 1105 16-17 0.6402921005484856 1105 18-19 0.7638684582178996 1105 20-21 1.161957489374899 1105 22-23 1.2787821069660978 1105 24-25 1.3978619908718173 1105 26-27 1.2094290427788366 1105 28-29 0.9791975027837552 1105 30-31 0.987415254087523 1105 32-33 1.1108783010921144 1105 34-35 1.6145191933372232 1105 36-37 1.5827543578448129 1105 38-39 1.7003531917046502 1105 40-41 1.287886143284176 1105 42-43 0.9622029647598893 1105 44-45 1.1144776856524459 1105 46-47 1.1271141062195937 1105 48-49 0.9736593515608689 1105 50-51 0.966900882927586 1105 52-53 0.9478587562110015 1105 54-55 0.8486387427753037 1105 56-57 0.8154428068287629 1105 58-59 0.8207174396759953 1105 60-61 0.8931659870710966 1105 62-63 0.8542491630256954 1105 64-65 0.8509066214195187 1105 66-67 0.6769290967417092 1105 68-69 0.45448410415923135 1105 70-71 0.6851105907106536 1105 72-73 0.7033349718266564 1105 74-75 0.6491208293188357 1105 76-77 0.65139192127074 1105 78-79 0.7164130358381193 1105 80-81 0.6687222888014652 1105 82-83 0.592597112340087 1105 84-85 0.5667872239377871 1105 86-87 0.6355987881115936 1105 88-89 0.7564785608879028 1105 90-91 0.6750911883811845 1105 92-93 0.6477925530926818 1105 94-95 0.7809619664974505 1105 96-97 0.8384081165782078 1105 98-99 0.7149398324267331 1105 100-101 0.7503845487589054 1105 102-103 0.6399697114852039 1105 104-105 0.8022178917513081 1105 106-107 0.8086035676430718 1105 108-109 0.7902493464598912 1105 110-111 0.864541916426834 1105 112-113 0.7698062632615574 1105 114-115 0.8021670557722409 1105 116-117 0.9415659657764728 1105 118-119 0.9473245220425142 1105 120-121 0.7644498778855251 1105 122-123 0.8645528041260668 1105 124-125 0.9320235268591368 1106 1 0.4676662990235876 1106 2 0.780137535303254 1106 3 0.5477163967935468 1106 4 0.5839452555029752 1106 5 0.5623251475306574 1106 6 0.5741554084981004 1106 7 0.38564008100102853 1106 8 0.6175797102958214 1106 9 0.8818496441486019 1106 10-11 0.9616177722005972 1106 12-13 0.9466018476895925 1106 14-15 1.20924988927311 1106 16-17 0.9680431483048366 1106 18-19 1.0606902238707292 1106 20-21 1.4405569087662542 1106 22-23 1.4092778401834707 1106 24-25 1.6568587082772481 1106 26-27 1.6963184236706539 1106 28-29 1.6040480926953435 1106 30-31 1.3845323472138205 1106 32-33 1.3616317739543007 1106 34-35 1.6126413596834794 1106 36-37 1.552970097595015 1106 38-39 1.7569360520745718 1106 40-41 1.4309835939714688 1106 42-43 1.3058267642046033 1106 44-45 1.2857077687711325 1106 46-47 1.0946602782276358 1106 48-49 1.072858358255715 1106 50-51 0.9278892993960035 1106 52-53 0.9548712190948798 1106 54-55 0.8001776748847966 1106 56-57 0.921048409890183 1106 58-59 0.8003407130835001 1106 60-61 0.7969441410478275 1106 62-63 0.7799842322380677 1106 64-65 0.7308657682685116 1106 66-67 0.6365729044027439 1106 68-69 0.7148756892256358 1106 70-71 0.8192788003979139 1106 72-73 0.8568418263799948 1106 74-75 0.8750474943544795 1106 76-77 0.7922663673848334 1106 78-79 0.7716648428065156 1106 80-81 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2216 106-107 -2.9362553628317407 2216 108-109 -3.067886556287032 2216 110-111 -3.0933787473487726 2216 112-113 -3.1411238871945244 2216 114-115 -3.2722481095667497 2216 116-117 -3.329350412514298 2216 118-119 -3.4577746208605156 2216 120-121 -3.488372060167368 2216 122-123 -3.485862968210288 2216 124-125 -3.4625291411133077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 158680.0 3 27943.0 4 17654.0 5 13803.0 6 13326.0 7 14159.0 8 15038.0 9 15878.0 10 17353.0 11 18305.0 12 19881.0 13 22181.0 14 25136.0 15 21828.0 16 16709.0 17 14761.0 18 14079.0 19 13025.0 20 13254.0 21 13852.0 22 16664.0 23 21828.0 24 29328.0 25 36787.0 26 45137.0 27 51509.0 28 59942.0 29 70113.0 30 80956.0 31 92418.0 32 114361.0 33 143504.0 34 186680.0 35 257166.0 36 396162.0 37 518297.0 38 181704.0 39 513.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66604649161957 18.603638240782068 13.281711826523058 23.448603441075306 2 15.307760000430145 20.034232396751687 40.60105636516468 24.056951237653486 3 19.042236940824836 25.674318834470206 28.681559483622955 26.601884741082 4 12.12526929708532 16.391365286933578 37.61136621894346 33.871999197037646 5 13.219227902678183 38.74958907920402 34.004775822764714 14.02640719535309 6 31.727894843241245 36.92913560811673 17.486590878786707 13.85637866985532 7 28.62091597684805 32.85765032836358 20.87077555172645 17.65065814306192 8 26.40981048161341 35.09724672516788 19.813549808345346 18.67939298487337 9 26.93561828703745 16.39392152610629 19.56868571551466 37.1017744713416 10-11 24.77524396809364 27.02803742337577 27.35702605886776 20.83969254966282 12-13 25.702337708516588 24.305888610974304 27.590591671308534 22.401182009200575 14-15 22.599106829488466 25.83834667735479 25.222082685491337 26.340463807665405 16-17 22.342681312503316 29.168835862508118 25.972122427262022 22.51636039772654 18-19 22.377979953164548 27.573252533276076 28.11715100221631 21.931616511343062 20-21 22.976743385754528 26.829175179442654 28.354583384937232 21.839498049865586 22-23 22.73627938953078 26.649638296484333 28.23362799630375 22.38045431768114 24-25 22.498665570205706 26.87505758033196 28.29550387674708 22.330772972715256 26-27 22.11074408489897 27.160493384565637 28.039127607294223 22.689634923241172 28-29 22.292206784398445 27.265806206026667 28.12175055674018 22.320236452834706 30-31 22.281340718605158 26.951349468094833 28.002506920555998 22.76480289274402 32-33 22.043976291230013 27.096697297365115 27.896465013074952 22.96286139832992 34-35 22.64771185881719 27.046268256521877 27.95074216184749 22.355277722813447 36-37 22.9654743855311 26.436353709970646 27.85733348699798 22.740838417500274 38-39 22.37664357694244 26.874596649681077 27.895488516041805 22.853271257334683 40-41 22.633435166558453 26.83438733888307 27.927980091789728 22.60419740276875 42-43 22.45953793486638 26.78542111017511 27.623375692291148 23.131665262667358 44-45 22.446460618288533 27.01814556459679 27.80853661771781 22.726857199396868 46-47 23.006161687850827 27.352212687556904 26.97297675118825 22.668648873404017 48-49 22.70357933653421 27.376568173604777 26.854560681936817 23.065291807924186 50-51 22.921902303949242 27.40923551182443 26.87017770250289 22.798684481723434 52-53 23.131178953903238 27.060045578465857 26.988932275331784 22.819843192299118 54-55 22.794413145455753 27.271694142657665 26.952091933584406 22.981800778302183 56-57 22.730859649226566 27.64458753680587 26.69181536425555 22.932737449712015 58-59 22.675485365989164 27.013625911993422 26.960039857987994 23.35084886402942 60-61 22.53394004142577 27.334751709749355 27.154918642294394 22.976389606530482 62-63 22.562808169879865 27.335108666716263 27.44727555642002 22.654807606983855 64-65 22.397660451831065 27.308273830382756 27.056501737363668 23.237563980422507 66-67 22.37313147421212 27.574025495485177 26.928618269010364 23.124224761292343 68-69 22.770113580611618 27.163858266855552 27.286772626002453 22.779255526530378 70-71 22.61570087227068 27.028723817989885 27.16437651554119 23.191198794198247 72-73 22.58733770819487 27.35000039432544 26.79281854505383 23.269843352425852 74-75 22.571291188777 27.782976067179142 26.723857209193046 22.921875534850813 76-77 22.78630587409587 27.316435736265287 27.03010342625572 22.867154963383125 78-79 22.744232518336315 27.39639143155414 26.904364423296638 22.95501162681291 80-81 22.835142552516164 27.606971962285176 26.685697684609266 22.87218780058939 82-83 22.968460756374796 27.566697916590126 26.767486052683033 22.697355274352045 84-85 22.685788880947584 27.67968833447913 26.697955564221694 22.936567220351595 86-87 22.81709916287126 27.592772747900007 26.766361719623166 22.823766369605565 88-89 22.688260586823677 27.715348286297914 26.589143840846504 23.00724728603191 90-91 22.535215812133575 27.823583758081327 26.769090177073036 22.872110252712062 92-93 22.72772078838683 27.471953166501663 26.811959145429164 22.98836689968234 94-95 22.531200865616636 27.158650313501003 27.128247608520716 23.181901212361637 96-97 22.815711661535417 27.006044932251733 27.10948848587747 23.06875492033538 98-99 22.38874795355018 27.32682352456233 26.839707981579934 23.444720540307557 100-101 22.30737920363396 27.09605691969827 27.25585993684949 23.340703939818283 102-103 22.37043865868267 26.699263848276328 27.29356532136833 23.636732171672676 104-105 22.144680057923637 27.141687097652945 27.120162874388864 23.593469970034555 106-107 22.366055859089514 27.103909691807594 26.96046435856602 23.569570090536875 108-109 22.496016006228146 26.87883927604937 26.786022794967874 23.839121922754607 110-111 22.28692201209078 27.146031354596268 27.093341180704357 23.47370545260859 112-113 22.30529328144165 27.22073153509391 27.290787457964655 23.18318772549978 114-115 22.09722235165672 27.577373352727 26.888732054106327 23.436672241509953 116-117 22.05523181001278 27.396633014494356 27.152145191572213 23.39598998392065 118-119 21.8951551911636 27.461599174741586 26.910399388654994 23.732846245439823 120-121 21.82422501629531 27.60945814269869 27.123797924200876 23.442518916805128 122-123 21.83827171733609 27.58145950018531 27.114402092680994 23.465866689797608 124-125 21.88782815886858 27.515314020781034 26.94937822530164 23.647479595048747 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1014.0 1 980.0 2 1157.0 3 1265.5 4 1024.5 5 822.0 6 788.5 7 901.5 8 1147.0 9 1312.0 10 1272.5 11 1240.5 12 1332.5 13 1497.5 14 1730.0 15 1940.5 16 2107.5 17 2296.5 18 2550.5 19 2808.0 20 3240.5 21 3937.5 22 4938.0 23 6447.0 24 8261.0 25 10651.0 26 14121.0 27 18206.5 28 22704.0 29 27615.0 30 33106.0 31 40838.5 32 50861.5 33 56944.5 34 58803.5 35 62171.0 36 68970.0 37 75519.5 38 78460.5 39 80312.5 40 81538.5 41 83752.5 42 85657.0 43 87727.5 44 90441.5 45 94361.0 46 95402.0 47 93188.0 48 92597.0 49 90859.5 50 90773.0 51 91311.5 52 91918.5 53 95212.5 54 97529.0 55 93499.5 56 86490.0 57 80596.5 58 76176.5 59 68646.5 60 56501.5 61 47356.5 62 40655.5 63 30873.5 64 21852.5 65 16773.0 66 12707.0 67 9924.5 68 7641.5 69 5759.5 70 4396.0 71 3109.0 72 2347.0 73 1936.0 74 1593.5 75 1228.0 76 856.0 77 565.0 78 395.5 79 257.5 80 165.0 81 129.5 82 98.0 83 70.5 84 50.0 85 32.5 86 22.0 87 23.5 88 23.0 89 20.5 90 18.0 91 12.0 92 10.0 93 8.5 94 8.0 95 6.0 96 3.5 97 4.0 98 2.5 99 2.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013584648128938743 2 0.005699100402377994 3 0.008853319493002294 4 0.008745789296731012 5 0.016021999244421156 6 7.885547726560747E-4 7 0.006129221187463126 8 0.0 9 0.002473194514239507 10-11 0.0 12-13 0.0031542190906242986 14-15 0.01881778434747451 16-17 0.030897009728615293 18-19 0.005268979617292863 20-21 0.008405277008538613 22-23 0.0021506039254256585 24-25 0.011935851786112403 26-27 0.018925314543745794 28-29 4.659641838422259E-4 30-31 0.0013262057540124892 32-33 0.008208138315374596 34-35 0.008100608119103313 36-37 0.0103766639401788 38-39 1.7921699378547154E-5 40-41 0.025556343313808242 42-43 0.009462657271872897 44-45 0.016918084213348512 46-47 0.0033692794831668644 48-49 0.024445197952338318 50-51 0.004982232427236108 52-53 0.0 54-55 0.018244289967361 56-57 0.0038531653663876377 58-59 3.2259058881384874E-4 60-61 0.003745635170116355 62-63 0.027473965147312786 64-65 0.01743781349532638 66-67 0.0017025614409619794 68-69 0.020556189187193583 70-71 0.002473194514239507 72-73 0.012186755577412063 74-75 1.7921699378547154E-5 76-77 0.0053227447154285045 78-79 9.49850067062999E-4 80-81 0.00295708039746028 82-83 0.0014516576496623192 84-85 0.0 86-87 0.005018075825993203 88-89 0.016344589833235005 90-91 0.021972003438098808 92-93 0.004157834255822939 94-95 0.024678180044259427 96-97 0.016846397415834324 98-99 7.706330732775276E-4 100-101 0.008226060014753143 102-103 0.0 104-105 0.0012186755577412063 106-107 3.584339875709431E-5 108-109 0.0 110-111 7.168679751418861E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.7921699378547154E-5 122-123 0.0 124-125 0.022939775204540356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2789914.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.435882270851465 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74942112282498 34.98368857925772 2 12.531686158877378 11.889031785755323 3 5.137223777151963 7.31066226875998 4 2.5807703748165425 4.896844786715949 5 1.5051165961155197 3.5698266828620246 6 0.9469534876222359 2.695174449289235 7 0.6791589329573363 2.2551552230872915 8 0.4841638815048983 1.8373392706291867 9 0.3552718789412489 1.5167371521241149 >10 1.8193876358224745 15.548161282584449 >50 0.1365962490532317 4.45514736097772 >100 0.06811379219477677 5.865568324473717 >500 0.0038059429589039906 1.2043375865655415 >1k 0.0023301691584921604 1.9723252469177146 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4261 0.15272872210397884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3231 0.1158100213841717 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3065 0.10986001719049406 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.3765098135641456E-5 0.0 12-13 0.0 0.0 0.0 7.16867975141886E-5 0.0 14-15 0.0 0.0 0.0 7.16867975141886E-5 0.0 16-17 0.0 0.0 0.0 1.792169937854715E-4 0.0 18-19 0.0 0.0 0.0 2.867471900567544E-4 0.0 20-21 0.0 0.0 0.0 5.376509813564145E-4 0.0 22-23 0.0 0.0 0.0 0.0010932236620913762 0.0 24-25 0.0 3.58433987570943E-5 0.0 0.0015950312446906968 0.0 26-27 0.0 3.58433987570943E-5 0.0 0.002007230330397281 0.0 28-29 0.0 3.58433987570943E-5 0.0 0.00258072471051079 0.0 30-31 0.0 3.58433987570943E-5 0.0 0.004301207850851316 0.0 32-33 0.0 3.58433987570943E-5 0.0 0.009086301584923406 0.0 34-35 0.0 3.58433987570943E-5 0.0 0.015018384079222513 0.0 36-37 0.0 3.58433987570943E-5 0.0 0.02505453573120892 0.0 38-39 0.0 3.58433987570943E-5 0.0 0.04242066242902111 0.0 40-41 0.0 3.58433987570943E-5 0.0 0.06458980456028393 0.0 42-43 0.0 3.58433987570943E-5 0.0 0.08706361558098206 0.0 44-45 0.0 3.58433987570943E-5 0.0 0.11213607301156953 0.0 46-47 0.0 3.58433987570943E-5 0.0 0.14305100443956337 0.0 48-49 0.0 3.58433987570943E-5 0.0 0.17930660228236425 0.0 50-51 0.0 3.58433987570943E-5 0.0 0.22133298732505735 0.0 52-53 0.0 3.58433987570943E-5 0.0 0.2629113298832867 0.0 54-55 0.0 3.58433987570943E-5 0.0 0.30542160080920056 0.0 56-57 0.0 3.58433987570943E-5 0.0 0.35741245070636585 0.0 58-59 0.0 3.58433987570943E-5 0.0 0.416177702968622 0.0 60-61 0.0 3.58433987570943E-5 0.0 0.48390380492015167 0.0 62-63 0.0 3.58433987570943E-5 0.0 0.5518270455648454 0.0 64-65 0.0 3.58433987570943E-5 0.0 0.6222951675212928 0.0 66-67 0.0 3.58433987570943E-5 0.0 0.7074053178700133 0.0 68-69 0.0 3.58433987570943E-5 0.0 0.8056341521638302 0.0 70-71 0.0 3.58433987570943E-5 0.0 0.9191322743281692 0.0 72-73 0.0 3.58433987570943E-5 0.0 1.0363939533620032 0.0 74-75 0.0 3.58433987570943E-5 0.0 1.1540678314815438 0.0 76-77 0.0 3.58433987570943E-5 0.0 1.283874700080361 0.0 78-79 0.0 3.58433987570943E-5 0.0 1.4377683326439454 0.0 80-81 0.0 3.58433987570943E-5 0.0 1.6103184542605973 0.0 82-83 0.0 3.58433987570943E-5 0.0 1.796650362699352 0.0 84-85 0.0 3.58433987570943E-5 0.0 1.9823191682611006 0.0 86-87 0.0 3.58433987570943E-5 0.0 2.1825045503194724 0.0 88-89 0.0 3.58433987570943E-5 0.0 2.400432414762606 0.0 90-91 0.0 3.58433987570943E-5 0.0 2.6588812415006338 0.0 92-93 0.0 3.58433987570943E-5 0.0 2.9343736043476607 0.0 94-95 0.0 3.58433987570943E-5 0.0 3.2062278622208424 0.0 96-97 0.0 7.16867975141886E-5 0.0 3.490573544560872 0.0 98-99 0.0 7.16867975141886E-5 0.0 3.80194514956375 0.0 100-101 0.0 7.16867975141886E-5 0.0 4.162726879753283 0.0 102-103 0.0 7.16867975141886E-5 0.0 4.555480921634143 0.0 104-105 0.0 7.16867975141886E-5 0.0 4.954435871499982 0.0 106-107 0.0 7.16867975141886E-5 0.0 5.351276060839151 0.0 108-109 0.0 7.16867975141886E-5 0.0 5.772220935842467 0.0 110-111 0.0 7.16867975141886E-5 0.0 6.242432562437408 0.0 112-113 0.0 7.16867975141886E-5 0.0 6.749365751058993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7610 0.0 57.9407 1 GGTATCA 4305 0.0 57.776775 1 ATCAACG 9530 0.0 46.264954 3 CAACGCA 9570 0.0 46.258106 5 TCAACGC 9720 0.0 45.483032 4 AACGCAG 10030 0.0 43.77124 6 TATCAAC 10115 0.0 43.648052 2 GTACATG 13620 0.0 36.829914 1 ACGCAGA 11860 0.0 36.716377 7 CGCAGAG 11860 0.0 35.96786 8 TACATGG 13905 0.0 35.5596 2 ACATGGG 13800 0.0 34.62289 3 GCAGAGT 13375 0.0 30.826723 9 CATGGGA 7665 0.0 28.25635 4 CATGGGG 6610 0.0 27.635271 4 AGAGTAC 12245 0.0 25.489668 10-11 CAGAGTA 12825 0.0 24.870522 10-11 GTATACG 195 1.4650795E-6 24.412125 1 GAGTACT 6715 0.0 23.751635 12-13 TAAGGTG 1660 0.0 23.298723 5 >>END_MODULE