FastQCFastQC Report
Thu 26 May 2016
SRR1364580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789914
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87680.3142749203022029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73930.26499024701119817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47610.170650421482526No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58450.062.0291181
GTATCAA99500.056.9946331
ATCAACG131400.043.521133
CAACGCA135300.042.127935
TCAACGC135350.042.0309684
TATCAAC134850.041.789242
AACGCAG139450.040.916886
ACGCAGA165200.034.359027
CGCAGAG167600.033.9025048
GTACATG131150.032.3279231
GTGGTAT24050.031.9853551
TACATGG134550.030.9469432
ACATGGG133400.030.4545963
GCAGAGT184100.030.023719
CATGGGA74650.028.5678464
TGGTATC25600.028.155922
TAAGGTG17850.025.6658085
GAGTACT116000.025.56875612-13
CAGAGTA177600.025.30874410-11
AGAGTAC173850.025.08466710-11
Produced by FastQC (version 0.11.5)