##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364580_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2789914 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.14362880002753 31.0 30.0 34.0 26.0 34.0 2 29.997727886952788 31.0 30.0 34.0 26.0 34.0 3 30.266798188044508 31.0 30.0 34.0 26.0 34.0 4 33.84181232826531 35.0 35.0 37.0 30.0 37.0 5 33.27825122924936 35.0 33.0 37.0 27.0 37.0 6 33.33575443544138 36.0 35.0 37.0 27.0 37.0 7 33.1228457221262 36.0 33.0 37.0 26.0 37.0 8 33.044577718166224 36.0 33.0 37.0 26.0 37.0 9 34.25785060041277 38.0 34.0 39.0 25.0 39.0 10-11 34.204822263338585 38.0 34.0 39.0 25.0 39.0 12-13 34.097115538328424 38.0 34.0 39.0 25.0 39.0 14-15 35.082799326430845 39.0 34.0 41.0 25.0 41.0 16-17 35.09972224950303 39.0 34.0 41.0 25.0 41.0 18-19 35.125665880740414 39.0 34.0 41.0 25.0 41.0 20-21 35.14336051218783 39.0 34.0 41.0 25.0 41.0 22-23 35.109718794199395 39.0 34.0 41.0 25.0 41.0 24-25 35.07524927291666 39.0 34.0 41.0 25.0 41.0 26-27 34.951554241456904 39.0 34.0 41.0 25.0 41.0 28-29 34.800724502619076 39.0 34.0 41.0 24.0 41.0 30-31 34.6250807372557 38.5 33.5 41.0 24.0 41.0 32-33 34.41580529005554 38.0 33.0 40.5 23.5 41.0 34-35 34.227389087979056 38.0 33.0 40.0 22.5 41.0 36-37 34.01628132623443 38.0 33.0 40.0 20.5 41.0 38-39 33.83626036501484 38.0 33.0 40.0 19.0 41.0 40-41 33.84323226450708 38.0 33.0 40.0 18.5 41.0 42-43 33.69299268722979 38.0 33.0 40.0 17.0 41.0 44-45 33.52467172823248 38.0 33.0 40.0 15.5 41.0 46-47 33.26456854942482 38.0 32.0 40.0 13.5 41.0 48-49 32.96067351896869 38.0 32.0 40.0 8.5 41.0 50-51 32.77414733930867 38.0 31.5 40.0 7.5 41.0 52-53 32.575037079996015 37.5 31.0 40.0 6.5 41.0 54-55 32.37614887053866 37.0 31.0 40.0 2.0 41.0 56-57 32.148900826333715 37.0 31.0 40.0 2.0 41.0 58-59 31.933133960401648 36.5 31.0 40.0 2.0 41.0 60-61 31.68711329453166 36.0 30.5 40.0 2.0 41.0 62-63 31.499273095873207 36.0 30.0 39.5 2.0 41.0 64-65 31.356282845994535 35.5 30.0 39.0 2.0 41.0 66-67 31.047382822552954 35.0 30.0 39.0 2.0 41.0 68-69 30.75863019433574 35.0 30.0 39.0 2.0 40.5 70-71 30.436759878619913 35.0 30.0 38.0 2.0 40.0 72-73 30.15921064233521 35.0 29.0 37.0 2.0 40.0 74-75 29.82270887202975 35.0 29.0 37.0 2.0 39.0 76-77 29.52417834384859 35.0 29.0 36.0 2.0 39.0 78-79 29.24609181501652 34.0 29.0 36.0 2.0 38.5 80-81 28.980525026936313 34.0 29.0 35.5 2.0 37.0 82-83 28.70321952576316 34.0 29.0 35.0 2.0 37.0 84-85 28.477461133210557 34.0 29.0 35.0 2.0 36.5 86-87 28.279507898809783 34.0 28.5 35.0 2.0 36.0 88-89 28.101265845470508 34.0 28.0 35.0 2.0 36.0 90-91 27.967440752654024 34.0 28.0 35.0 2.0 35.5 92-93 27.853750868306335 34.0 27.5 35.0 2.0 35.0 94-95 27.742392776264786 34.0 27.0 35.0 2.0 35.0 96-97 27.62899644935292 34.0 27.0 35.0 2.0 35.0 98-99 27.545194224624844 34.0 27.0 35.0 2.0 35.0 100-101 27.446311427520705 34.0 27.0 35.0 2.0 35.0 102-103 26.157247320168292 31.5 24.5 34.0 2.0 35.0 104-105 27.107556361952376 33.5 25.5 35.0 2.0 35.0 106-107 27.2728024949873 34.0 27.0 35.0 2.0 35.0 108-109 27.280745212934878 34.0 27.0 35.0 2.0 35.0 110-111 27.190507664393955 34.0 27.0 35.0 2.0 35.0 112-113 27.10829043475892 34.0 26.5 35.0 2.0 35.0 114-115 27.010663948781215 34.0 26.0 35.0 2.0 35.0 116-117 26.885936448220267 34.0 25.0 35.0 2.0 35.0 118-119 26.74899728091977 34.0 25.0 35.0 2.0 35.0 120-121 26.554631253866606 34.0 25.0 35.0 2.0 35.0 122-123 26.26421943472093 34.0 25.0 35.0 2.0 35.0 124-125 24.475759826288552 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5829579797619644 1101 2 0.6870746467356383 1101 3 0.49801334073064396 1101 4 0.39558414826476707 1101 5 0.5718443738473766 1101 6 0.5596878726070784 1101 7 0.6374263832011096 1101 8 0.6201311213215632 1101 9 0.7741461006829411 1101 10-11 0.7464990981715758 1101 12-13 0.6644247677706048 1101 14-15 0.8023324527632312 1101 16-17 0.8274581109788315 1101 18-19 0.877611658215308 1101 20-21 0.8974752009387004 1101 22-23 0.804054510482267 1101 24-25 0.912377394867427 1101 26-27 0.9088881709376935 1101 28-29 0.9485703346645238 1101 30-31 0.9352045882714535 1101 32-33 1.0546629958054865 1101 34-35 1.017257737738575 1101 36-37 1.019236764911156 1101 38-39 1.041613631842587 1101 40-41 0.7604176241016916 1101 42-43 0.7953747514789455 1101 44-45 0.7489786141140584 1101 46-47 0.944853057476891 1101 48-49 0.8450592565190007 1101 50-51 0.9056437058351179 1101 52-53 0.8271075128960774 1101 54-55 0.8753198904546338 1101 56-57 0.8792616906275654 1101 58-59 0.883783993299005 1101 60-61 0.893150892358193 1101 62-63 0.843638068322214 1101 64-65 0.7813074071202379 1101 66-67 0.9163063660147657 1101 68-69 0.9957988582176078 1101 70-71 0.9231030480403355 1101 72-73 0.8664977862031478 1101 74-75 0.9224982430137594 1101 76-77 0.8807290373955929 1101 78-79 0.8714964976097335 1101 80-81 0.7303004506884996 1101 82-83 0.967560066196171 1101 84-85 0.8682243506829153 1101 86-87 0.8482154308883381 1101 88-89 0.8630908708691933 1101 90-91 0.8907620577458957 1101 92-93 0.8893792172798776 1101 94-95 0.8092541058499592 1101 96-97 0.9187831688706893 1101 98-99 0.9345628196546798 1101 100-101 0.9552645486391178 1101 102-103 0.9088135824184533 1101 104-105 0.8582562877642452 1101 106-107 0.8576967798581201 1101 108-109 0.8555159015997695 1101 110-111 0.814068764095282 1101 112-113 0.8697709954592554 1101 114-115 0.8226669071104098 1101 116-117 0.8257500742221673 1101 118-119 0.8449734482013191 1101 120-121 0.8723147411224978 1101 122-123 0.7608396842264824 1101 124-125 1.0687596791299079 1102 1 0.5561098395761164 1102 2 -0.1345358930130658 1102 3 0.3480716794288128 1102 4 0.4310552314129481 1102 5 0.6966877729032035 1102 6 0.5395648989103776 1102 7 0.5385362051919529 1102 8 0.7433863516261283 1102 9 1.0695341682088895 1102 10-11 1.1361566324103904 1102 12-13 1.10274360802876 1102 14-15 1.3462645149804544 1102 16-17 1.2707873324348427 1102 18-19 1.354735722840573 1102 20-21 1.2333293090256348 1102 22-23 1.3672044107682026 1102 24-25 1.3162766296099662 1102 26-27 1.3229422559540538 1102 28-29 0.9974573939560472 1102 30-31 1.3177435504505155 1102 32-33 1.4041029397749725 1102 34-35 1.3407550167564892 1102 36-37 1.0579727378984245 1102 38-39 0.4486002555437807 1102 40-41 0.13779383506692255 1102 42-43 0.46938680003041355 1102 44-45 0.21806711313243454 1102 46-47 0.47397528619062257 1102 48-49 0.18132075063493147 1102 50-51 0.0869291303073112 1102 52-53 -0.19077510649619 1102 54-55 -0.06314028261550675 1102 56-57 -0.02613781144171412 1102 58-59 -5.8923897957186E-4 1102 60-61 -0.01569988679483103 1102 62-63 0.07687340449110991 1102 64-65 0.0384942400415369 1102 66-67 -0.1748814567456236 1102 68-69 0.14846973705865452 1102 70-71 -0.43756136447080607 1102 72-73 -0.1496017304352364 1102 74-75 -0.08318922819753638 1102 76-77 -0.4193549359163349 1102 78-79 -0.31853701473761475 1102 80-81 -0.32046468791218174 1102 82-83 -0.5017161218909152 1102 84-85 -0.3894856174065495 1102 86-87 -0.15362076364674238 1102 88-89 -0.2025896621858756 1102 90-91 -0.20984630161731133 1102 92-93 -0.09442113883126524 1102 94-95 0.09560193920153992 1102 96-97 -0.20854592172370445 1102 98-99 -0.3540956665005339 1102 100-101 -0.11461748006462713 1102 102-103 -0.1961888010515942 1102 104-105 -0.039313619904735475 1102 106-107 0.06411309707936397 1102 108-109 0.08267053078990472 1102 110-111 0.13375976426859637 1102 112-113 0.2973278260225527 1102 114-115 0.2877092803515211 1102 116-117 0.1962916522751783 1102 118-119 0.46783937651111174 1102 120-121 0.6321054485665165 1102 122-123 0.6570315262271542 1102 124-125 0.757649029631775 1103 1 0.17130373931612652 1103 2 -0.2842596073458026 1103 3 0.08119904299716652 1103 4 0.0763438850467324 1103 5 0.20480283578216074 1103 6 0.17914551253767996 1103 7 0.2693448824484861 1103 8 0.3344973021767075 1103 9 0.46327348337186436 1103 10-11 0.49278529202450017 1103 12-13 0.49535662497642363 1103 14-15 0.5186535533772059 1103 16-17 0.5360773734473057 1103 18-19 0.5319691099560302 1103 20-21 0.5685005799069174 1103 22-23 0.5427296373437187 1103 24-25 0.44520498473006853 1103 26-27 0.5288501429418275 1103 28-29 0.5258592518675442 1103 30-31 0.5060969134276192 1103 32-33 0.5307920408185964 1103 34-35 0.5417676731666177 1103 36-37 0.5500370335722238 1103 38-39 0.5792023976813496 1103 40-41 0.3987755960122996 1103 42-43 0.44464136903761187 1103 44-45 0.4597313219226322 1103 46-47 0.40132888169782177 1103 48-49 0.3372668357649147 1103 50-51 0.34210414043985793 1103 52-53 0.3011107351912372 1103 54-55 0.31887305374586106 1103 56-57 0.34754624941457735 1103 58-59 0.4482472427253157 1103 60-61 0.4357296475123462 1103 62-63 0.3440387812178507 1103 64-65 0.2992250403515513 1103 66-67 0.29779891421478766 1103 68-69 0.34888913926126364 1103 70-71 0.3112143756015904 1103 72-73 0.3002264669680592 1103 74-75 0.4202285062673461 1103 76-77 0.4939160874925399 1103 78-79 0.3848059460087825 1103 80-81 0.4064513292616887 1103 82-83 0.5275460351844323 1103 84-85 0.5286855834273716 1103 86-87 0.5301536843091164 1103 88-89 0.564818421007999 1103 90-91 0.5655874619899777 1103 92-93 0.5280377575310382 1103 94-95 0.4430327134430847 1103 96-97 0.4881876551528208 1103 98-99 0.4654362170051094 1103 100-101 0.46012110166461184 1103 102-103 0.4061724111872138 1103 104-105 0.4862032931403739 1103 106-107 0.513516374425123 1103 108-109 0.554129624065002 1103 110-111 0.4959165965178336 1103 112-113 0.4427395289518721 1103 114-115 0.549818272346279 1103 116-117 0.5387712258942479 1103 118-119 0.42243070953270845 1103 120-121 0.3900073588580675 1103 122-123 0.3969511374128629 1103 124-125 0.6863057378140773 1104 1 0.39068833203022635 1104 2 0.5812309720902391 1104 3 0.3559876689060424 1104 4 0.1299358561492241 1104 5 0.3617288133560024 1104 6 0.4845580461554633 1104 7 0.6276136032841464 1104 8 0.3605557533662136 1104 9 0.4235165938784746 1104 10-11 0.48665758771635836 1104 12-13 0.5063806259804622 1104 14-15 0.6245386150335221 1104 16-17 0.6388672998015537 1104 18-19 0.524108035459065 1104 20-21 0.3867712167193531 1104 22-23 0.519979853143063 1104 24-25 0.630823430685119 1104 26-27 0.5300386903868883 1104 28-29 0.6986228834031465 1104 30-31 0.5903304296221634 1104 32-33 0.7394873714951302 1104 34-35 0.8401654084099235 1104 36-37 0.9134858936217398 1104 38-39 0.9892023059139134 1104 40-41 0.8530954897657637 1104 42-43 0.8408456874858956 1104 44-45 0.816011765968824 1104 46-47 0.8873002397545733 1104 48-49 0.9219291658101625 1104 50-51 0.7620773920885071 1104 52-53 0.9260633198051877 1104 54-55 0.6202348241635676 1104 56-57 0.6508101519293348 1104 58-59 0.7925592768364993 1104 60-61 0.7898039864565973 1104 62-63 0.8773101794878428 1104 64-65 0.8756031047938251 1104 66-67 0.9023645935221793 1104 68-69 0.5799990642915098 1104 70-71 0.12941045800195994 1104 72-73 0.16409170187174738 1104 74-75 0.6268849819744595 1104 76-77 0.7088839877204371 1104 78-79 0.7427867398798647 1104 80-81 0.6756647916189813 1104 82-83 0.7098270373675959 1104 84-85 0.7128454841021181 1104 86-87 0.7582542739387677 1104 88-89 0.7254224692887021 1104 90-91 0.7479559880293607 1104 92-93 0.7114334953738535 1104 94-95 0.7687195383390879 1104 96-97 0.8374926865598269 1104 98-99 0.8497871265609795 1104 100-101 0.7542241738951851 1104 102-103 0.6749527100233621 1104 104-105 0.7240385512824403 1104 106-107 0.7746400332745296 1104 108-109 0.7664691033235194 1104 110-111 0.7672534141493585 1104 112-113 0.8304164246111831 1104 114-115 0.7527020720146709 1104 116-117 0.7187018412246182 1104 118-119 0.7315969556870279 1104 120-121 0.7014152501044286 1104 122-123 0.7209368168125536 1104 124-125 1.0999808523363903 1105 1 0.2670358405025972 1105 2 0.0679736695887847 1105 3 0.24631046099564102 1105 4 0.301989238724218 1105 5 -0.033902739733967735 1105 6 0.3337681928029923 1105 7 0.3927219818730663 1105 8 0.5183123611300715 1105 9 0.6557282219163412 1105 10-11 0.5112880383012026 1105 12-13 0.5931079848757648 1105 14-15 0.7033411789363839 1105 16-17 0.5566615991252135 1105 18-19 0.672085132070606 1105 20-21 0.6125608834353855 1105 22-23 0.781390474117174 1105 24-25 0.6147509359033947 1105 26-27 0.6257684115965247 1105 28-29 0.6078897505852936 1105 30-31 0.7558815310233555 1105 32-33 0.7133200955818353 1105 34-35 0.8744057030635872 1105 36-37 0.8310978187001012 1105 38-39 0.9051045882631286 1105 40-41 0.6521979352749412 1105 42-43 0.7134043438772011 1105 44-45 0.7843439303315094 1105 46-47 0.7203925164633986 1105 48-49 0.6776264879928888 1105 50-51 0.4858961780070885 1105 52-53 0.6313706565445045 1105 54-55 0.49398392983087547 1105 56-57 0.49500938815478435 1105 58-59 0.21877884801531877 1105 60-61 0.24516483887842355 1105 62-63 0.5370604879939052 1105 64-65 0.2414906865452231 1105 66-67 0.41857480217007037 1105 68-69 0.5491787434007307 1105 70-71 0.6064058093515392 1105 72-73 0.582286462105337 1105 74-75 0.4811135309748913 1105 76-77 0.4442086321436065 1105 78-79 0.4283106405422643 1105 80-81 0.44021124892315555 1105 82-83 0.4194375220891686 1105 84-85 0.3785151062604868 1105 86-87 0.5756461109745032 1105 88-89 0.46123475104651135 1105 90-91 0.3293469213741034 1105 92-93 0.36246745859943275 1105 94-95 0.38838605793800696 1105 96-97 0.45564983359444966 1105 98-99 0.3736106458973367 1105 100-101 0.42276516457834745 1105 102-103 0.01751841412428945 1105 104-105 0.2954423966919819 1105 106-107 0.31660382029890144 1105 108-109 0.3804017014663579 1105 110-111 0.2449230392727344 1105 112-113 0.15483121877607786 1105 114-115 0.19241656962088172 1105 116-117 -0.04830204880823885 1105 118-119 0.15974248255163914 1105 120-121 0.20044844687636143 1105 122-123 0.21833420476013643 1105 124-125 0.6262560332321065 1106 1 -0.2551015037441502 1106 2 -0.5790000532569479 1106 3 -0.11496821606671048 1106 4 -0.34133113826473505 1106 5 -0.040897602453782156 1106 6 0.12466747591879823 1106 7 0.18232508396210534 1106 8 0.19263903460505105 1106 9 0.28392087527990384 1106 10-11 0.22268962135397175 1106 12-13 0.2989712007733658 1106 14-15 0.24264319250446675 1106 16-17 0.1978530668280385 1106 18-19 0.17008341403223426 1106 20-21 0.21863532316531575 1106 22-23 0.24090564206974818 1106 24-25 0.33204775403423525 1106 26-27 0.43091724120747443 1106 28-29 0.5299689869611868 1106 30-31 0.47506875435369267 1106 32-33 0.41790842009212525 1106 34-35 0.468208483058703 1106 36-37 0.5003700475433348 1106 38-39 0.4111527315988681 1106 40-41 0.5136147353639657 1106 42-43 0.38594665040821496 1106 44-45 0.413929292329712 1106 46-47 0.37222749207788297 1106 48-49 0.5638514572254039 1106 50-51 0.6309427922055875 1106 52-53 0.4399520647369073 1106 54-55 0.367244437839382 1106 56-57 0.0058324505352302936 1106 58-59 0.46130643053330544 1106 60-61 0.4936470706929157 1106 62-63 0.31727248142346554 1106 64-65 0.49816273383347465 1106 66-67 0.40554119744439276 1106 68-69 0.44341197858854997 1106 70-71 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-1.8344989088996186 2216 54-55 -1.8729850621988966 2216 56-57 -1.7598232547451182 2216 58-59 -1.830033882867916 2216 60-61 -1.65693290021283 2216 62-63 -1.8124438496384734 2216 64-65 -1.7059200316515586 2216 66-67 -1.5620573146654486 2216 68-69 -1.4005040691872708 2216 70-71 -1.5232112295442661 2216 72-73 -1.3879489736082178 2216 74-75 -1.4022615725379772 2216 76-77 -1.4616035762212753 2216 78-79 -1.428772768405004 2216 80-81 -1.3286733047669124 2216 82-83 -1.2903527874377723 2216 84-85 -1.269713389658449 2216 86-87 -1.436311658904664 2216 88-89 -1.436919903955797 2216 90-91 -1.4548419705741082 2216 92-93 -1.393011161952117 2216 94-95 -1.3134970940261859 2216 96-97 -1.4243399298434483 2216 98-99 -1.4542768676870992 2216 100-101 -1.493901919268641 2216 102-103 -1.2959943432498982 2216 104-105 -1.3360834246839204 2216 106-107 -1.3045586943806313 2216 108-109 -1.3566019582479996 2216 110-111 -1.370716607667564 2216 112-113 -1.359439607386971 2216 114-115 -1.5060295901299767 2216 116-117 -1.4847778189752567 2216 118-119 -1.5570740300711954 2216 120-121 -1.6689435805991302 2216 122-123 -1.6608924055037484 2216 124-125 -2.0670375323872285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81273.0 3 26872.0 4 12886.0 5 10501.0 6 11547.0 7 14367.0 8 15891.0 9 17787.0 10 19839.0 11 21404.0 12 23563.0 13 27105.0 14 30850.0 15 27079.0 16 19136.0 17 16561.0 18 15120.0 19 14614.0 20 14520.0 21 15073.0 22 16751.0 23 20615.0 24 26869.0 25 35484.0 26 44022.0 27 49486.0 28 57315.0 29 68099.0 30 78736.0 31 92140.0 32 112304.0 33 139618.0 34 180216.0 35 247987.0 36 398439.0 37 577094.0 38 208164.0 39 587.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43279984127628 19.620498178659624 14.11955871647616 23.82714326358794 2 15.512557017886571 20.942652712592576 38.93625394904646 24.608536320474393 3 19.086466464557688 25.574874350965658 29.389293003296874 25.949366181179776 4 12.900466574145966 17.133037496717048 36.591877505270666 33.37461842386632 5 12.914770849567406 39.04127510740474 33.90710251283731 14.136851530190537 6 31.719974164078174 37.51911349238722 16.83460493764324 13.926307405891365 7 28.04293966596269 33.18200000860256 20.858836332041516 17.916223993393235 8 26.445365903666882 35.27657039377326 19.33609054197144 18.941973160588404 9 26.658331651634125 15.986346155707304 19.52734156153209 37.82798063112648 10-11 24.974098538639637 27.031770487872237 27.013615718553403 20.98051525493472 12-13 25.95431257020826 24.168934956418013 27.746607959958624 22.13014451341511 14-15 22.787982494797777 25.537565949625318 25.64457643579272 26.02987511978418 16-17 22.082115998848707 28.805619685530736 26.541350661509505 22.570913654111052 18-19 22.09499217168386 26.743682147424686 29.31596861791509 21.84535706297637 20-21 22.72184076470563 25.992926587628734 29.576086317567324 21.70914633009831 22-23 22.45861341962512 25.75751438933243 29.610464695327526 22.173407495714923 24-25 22.283285434604792 26.1476697848034 29.494278318256406 22.0747664623354 26-27 21.82418526162455 26.42206892398834 29.440369846525737 22.313375967861372 28-29 22.00519442534788 26.445510506775477 29.502145944283587 22.04714912359306 30-31 22.025212963553713 26.152849155923803 29.418935494068993 22.403002386453487 32-33 21.7426952945503 26.30708330077558 29.345599900211976 22.604621504462145 34-35 22.326835164094664 26.400795866826005 29.114571273523126 22.15779769555621 36-37 22.643206923224156 25.84335932935567 29.058189607278216 22.455244140141957 38-39 22.213104059838404 26.23752918548744 29.021611418846604 22.527755335827557 40-41 22.311064068641542 26.14116420792899 29.212405830430615 22.33536589299885 42-43 22.319971153232682 26.053724236661058 28.79744680301973 22.82885780708653 44-45 22.276349736945296 26.296527419841613 28.88320213454608 22.543920708667006 46-47 22.831725278987093 26.63350913325644 28.05676088940376 22.47800469835271 48-49 22.630930559149853 26.766022178461412 27.723363515864648 22.879683746524087 50-51 22.853356770137 26.845182324616456 27.61672940456229 22.68473150068425 52-53 23.061409778222554 26.559546279921175 27.687502195408175 22.6915417464481 54-55 22.752314229040753 26.746147013850607 27.64870171625362 22.85283704085502 56-57 22.681330325372702 27.03253762893148 27.38112243626888 22.905009609426934 58-59 22.526317751441855 26.57089721493053 27.691674362596864 23.21111067103075 60-61 22.481696568424688 26.883463074489033 27.814101079818233 22.820739277268046 62-63 22.491230912494075 26.851634136392732 28.111207012115784 22.545927938997405 64-65 22.291125318267607 26.71535643552468 27.84727527802435 23.146242968183365 66-67 22.27335681314908 27.089974816428033 27.663236214449622 22.973432155973267 68-69 22.644268361725192 26.733409476673742 27.898033397809645 22.72428876379142 70-71 22.54644766827938 26.67813416470902 27.757826943769594 23.017591223242007 72-73 22.528794794391512 26.976207868773017 27.330376491891865 23.164620844943606 74-75 22.509446172810254 27.322598965943595 27.223813585248003 22.944141275998145 76-77 22.66512516156412 26.985347935456076 27.48844588041065 22.861081022569156 78-79 22.69991118005788 27.00733786059355 27.396005755016105 22.896745204332465 80-81 22.801800127494992 27.220485509676195 27.097381334582593 22.880333028246216 82-83 22.935563605186395 27.189547778175243 27.130907977808633 22.74398063882973 84-85 22.584208688869982 27.275589856891646 27.149600310260457 22.990601143977916 86-87 22.774107015485065 27.154116578503857 27.123219568775237 22.94855683723584 88-89 22.627073854517583 27.276300246945794 26.950856550103257 23.14576934843337 90-91 22.42608955438941 27.424076051103373 27.12179069351075 23.028043700996463 92-93 22.591862633893438 27.12841887859187 27.15218352471998 23.12753496279471 94-95 22.413092303203612 26.838443765650123 27.358316421223023 23.390147509923246 96-97 22.6847698852908 26.651585032332253 27.400337634084448 23.2633074482925 98-99 22.317273659064725 26.96923419650879 27.089384692891283 23.624107451535206 100-101 22.23392926334534 26.7964732394245 27.41714754348681 23.55244995374335 102-103 22.31631512655946 26.390007003800115 27.389320961147906 23.90435690849252 104-105 22.061952447279737 26.808747509779874 27.24028410911591 23.889015933824485 106-107 22.297974335404692 26.818589868468994 27.031950720363945 23.85148507576237 108-109 22.389583046212724 26.60647005722579 26.887321058520275 24.116625838041212 110-111 22.237013611363658 26.79111864301072 27.1659692431104 23.805898502515223 112-113 22.284862544150105 26.889879042866554 27.249334567302075 23.575923845681267 114-115 21.99736264271945 27.242972364022688 26.896438384839104 23.863226608418753 116-117 21.974924675097512 27.087591230410684 27.11904381282004 23.818440281671766 118-119 21.755688526599744 27.16175122243911 26.845343619910867 24.237216631050277 120-121 21.71711071045812 27.278369398076624 26.99176311610852 24.012756775356735 122-123 21.722236609939234 27.26829228950795 26.911134390337665 24.098336710215147 124-125 21.64418111141606 27.283831029169335 26.764160877107066 24.30782698230754 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 328.0 1 262.0 2 374.5 3 592.5 4 458.5 5 353.0 6 690.5 7 1708.5 8 2940.5 9 3327.5 10 2619.0 11 1805.0 12 1629.0 13 1696.5 14 1923.5 15 2216.0 16 2463.5 17 2780.5 18 3191.5 19 3561.0 20 3970.0 21 4645.5 22 5671.5 23 7095.0 24 8960.5 25 11329.5 26 14684.0 27 18736.0 28 23309.0 29 28462.5 30 34147.5 31 42424.5 32 52512.0 33 57926.5 34 59278.5 35 62335.5 36 68883.0 37 75170.0 38 78233.0 39 80592.5 40 81547.0 41 82700.0 42 84619.5 43 86640.5 44 89353.0 45 93512.0 46 94678.0 47 93040.5 48 92534.0 49 90815.5 50 90288.5 51 90465.0 52 91163.5 53 94471.0 54 97258.5 55 93410.5 56 86267.0 57 80129.0 58 75649.5 59 68212.0 60 55904.0 61 46766.0 62 40051.0 63 30120.5 64 21184.5 65 16350.0 66 12110.5 67 9403.5 68 7296.5 69 5287.5 70 3980.5 71 2863.0 72 2177.0 73 1806.0 74 1461.5 75 1141.5 76 808.0 77 487.5 78 311.0 79 176.0 80 117.5 81 82.0 82 54.5 83 38.5 84 26.5 85 11.5 86 7.0 87 4.5 88 3.0 89 1.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09609615206776984 2 0.0 3 0.0 4 0.10000308253229312 5 0.0 6 0.0 7 0.001648796342826338 8 0.004874702230964825 9 0.002759941704296261 10-11 4.838858832207731E-4 12-13 0.0 14-15 1.7921699378547154E-5 16-17 2.5090379129966015E-4 18-19 0.0015054227477979607 20-21 0.0010753019627128292 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.00736581844458288 58-59 6.989462757633389E-4 60-61 0.0 62-63 0.0 64-65 1.9713869316401866E-4 66-67 0.0 68-69 1.7921699378547154E-5 70-71 0.0 72-73 0.0 74-75 9.856934658200934E-4 76-77 0.0 78-79 0.0 80-81 1.7921699378547154E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010932236620913765 90-91 1.7921699378547154E-5 92-93 0.001989308631018734 94-95 0.0 96-97 0.007437505242097069 98-99 0.002849550201188997 100-101 0.0016129529440692435 102-103 0.0 104-105 0.0 106-107 0.001415814250905225 108-109 1.7921699378547154E-5 110-111 8.960849689273577E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.602415701702633E-4 122-123 0.003996538961416015 124-125 0.09208169140697527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2789914.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.12140623383059 #Duplication Level Percentage of deduplicated Percentage of total 1 72.03791344687744 31.784140434272068 2 12.952657102670212 11.42978891668847 3 5.391926196830559 7.136980983395828 4 2.8368755850869833 5.0066776049783455 5 1.69387627542192 3.736810162886922 6 1.069203619855011 2.830486035498307 7 0.7478366711174529 2.309692389405009 8 0.5577520101271237 1.9687042413243534 9 0.39434133891969514 1.5659004968341685 >10 2.064251792912301 16.531924914870018 >50 0.1600511850440605 4.900977245190507 >100 0.08613201744029901 6.870603647067651 >500 0.004489223560541764 1.3565692889663776 >1k 0.0025302896432033433 1.9887394367872426 >5k 1.6324449310989314E-4 0.5820042018347179 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8768 0.3142749203022029 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7393 0.26499024701119817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4761 0.170650421482526 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.792169937854715E-5 0.0 0.0 5.3765098135641456E-5 0.0 12-13 3.58433987570943E-5 0.0 0.0 7.16867975141886E-5 0.0 14-15 3.58433987570943E-5 0.0 0.0 7.16867975141886E-5 0.0 16-17 3.58433987570943E-5 0.0 0.0 1.792169937854715E-4 0.0 18-19 3.58433987570943E-5 0.0 0.0 3.2259058881384874E-4 0.0 20-21 3.58433987570943E-5 0.0 0.0 5.734943801135088E-4 0.0 22-23 3.58433987570943E-5 0.0 0.0 0.0011469887602270179 0.0 24-25 3.58433987570943E-5 0.0 0.0 0.001648796342826338 0.0 26-27 3.58433987570943E-5 0.0 0.0 0.0020789171279114696 0.0 28-29 3.58433987570943E-5 0.0 0.0 0.0026703332074035258 0.0 30-31 3.58433987570943E-5 0.0 0.0 0.004516268243393883 0.0 32-33 3.58433987570943E-5 0.0 0.0 0.009337205376223066 0.0 34-35 3.58433987570943E-5 0.0 0.0 0.015251366171143626 0.0 36-37 3.58433987570943E-5 0.0 0.0 0.025179987626858746 0.0 38-39 3.58433987570943E-5 0.0 0.0 0.04263572282156368 0.0 40-41 3.58433987570943E-5 0.0 0.0 0.06503784704474762 0.0 42-43 3.58433987570943E-5 0.0 0.0 0.08729659767290318 0.0 44-45 3.58433987570943E-5 0.0 0.0 0.11233321170473355 0.0 46-47 3.58433987570943E-5 0.0 0.0 0.14258504025572116 0.0 48-49 3.58433987570943E-5 0.0 0.0 0.17808792672462306 0.0 50-51 3.58433987570943E-5 0.0 0.0 0.21925407019714588 0.0 52-53 3.58433987570943E-5 0.0 0.0 0.26006177968209776 0.0 54-55 3.58433987570943E-5 0.0 0.0 0.301210001455242 0.0 56-57 3.58433987570943E-5 0.0 0.0 0.3522330795859657 0.0 58-59 3.58433987570943E-5 0.0 0.0 0.41010224687929453 0.0 60-61 3.58433987570943E-5 0.0 0.0 0.47748783654263177 0.0 62-63 3.58433987570943E-5 0.0 0.0 0.5443895403227483 0.0 64-65 3.58433987570943E-5 0.0 0.0 0.6138361254146185 0.0 66-67 3.58433987570943E-5 0.0 0.0 0.6977813653037334 0.0 68-69 3.58433987570943E-5 0.0 0.0 0.7952754099230299 0.0 70-71 3.58433987570943E-5 0.0 0.0 0.9076086216277635 0.0 72-73 3.58433987570943E-5 0.0 0.0 1.0225584014417648 0.0 74-75 3.58433987570943E-5 0.0 0.0 1.1376515548507946 0.0 76-77 3.58433987570943E-5 0.0 0.0 1.2654332714198357 0.0 78-79 3.58433987570943E-5 0.0 0.0 1.4180365416281648 0.0 80-81 3.58433987570943E-5 0.0 0.0 1.5882030772274702 0.0 82-83 3.58433987570943E-5 0.0 0.0 1.7710760976861653 0.0 84-85 3.58433987570943E-5 0.0 0.0 1.9537340577523177 0.0 86-87 3.58433987570943E-5 1.792169937854715E-5 0.0 2.151930131179671 0.0 88-89 3.58433987570943E-5 3.58433987570943E-5 0.0 2.3681733558812206 0.0 90-91 3.58433987570943E-5 3.58433987570943E-5 0.0 2.6241310664056314 0.0 92-93 3.58433987570943E-5 3.58433987570943E-5 0.0 2.8973115300328254 0.0 94-95 3.58433987570943E-5 3.58433987570943E-5 0.0 3.168251781237701 0.0 96-97 3.58433987570943E-5 3.58433987570943E-5 0.0 3.4501780341616266 0.0 98-99 3.58433987570943E-5 3.58433987570943E-5 0.0 3.7584671068713944 0.0 100-101 3.58433987570943E-5 3.58433987570943E-5 0.0 4.116650190651038 0.0 102-103 3.58433987570943E-5 3.58433987570943E-5 0.0 4.507522454097151 0.0 104-105 3.58433987570943E-5 3.58433987570943E-5 0.0 4.902552551799088 0.0 106-107 3.58433987570943E-5 3.58433987570943E-5 0.0 5.297636414599159 0.0 108-109 3.58433987570943E-5 7.16867975141886E-5 0.0 5.7151940884199295 0.0 110-111 3.58433987570943E-5 7.16867975141886E-5 0.0 6.184617160242215 0.0 112-113 3.58433987570943E-5 7.16867975141886E-5 0.0 6.689776100625323 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5845 0.0 62.029118 1 GTATCAA 9950 0.0 56.994633 1 ATCAACG 13140 0.0 43.52113 3 CAACGCA 13530 0.0 42.12793 5 TCAACGC 13535 0.0 42.030968 4 TATCAAC 13485 0.0 41.78924 2 AACGCAG 13945 0.0 40.91688 6 ACGCAGA 16520 0.0 34.35902 7 CGCAGAG 16760 0.0 33.902504 8 GTACATG 13115 0.0 32.327923 1 GTGGTAT 2405 0.0 31.985355 1 TACATGG 13455 0.0 30.946943 2 ACATGGG 13340 0.0 30.454596 3 GCAGAGT 18410 0.0 30.02371 9 CATGGGA 7465 0.0 28.567846 4 TGGTATC 2560 0.0 28.15592 2 TAAGGTG 1785 0.0 25.665808 5 GAGTACT 11600 0.0 25.568756 12-13 CAGAGTA 17760 0.0 25.308744 10-11 AGAGTAC 17385 0.0 25.084667 10-11 >>END_MODULE