FastQCFastQC Report
Thu 26 May 2016
SRR1364579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343571
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.1526729934787553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.10270651070524427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76500.047.9912221
GGTATCA48650.044.2755361
TCAACGC90100.039.5559924
ATCAACG90900.039.469693
CAACGCA90900.038.9460455
AACGCAG92700.038.4408076
TATCAAC95700.037.552192
GTACATG125750.035.4414831
TACATGG129600.033.605962
ACATGGG129900.032.7038843
ACGCAGA110650.031.7747027
CGCAGAG110100.031.233678
CATGGGG49200.027.9355434
GCAGAGT119100.027.2754949
CATGGGA83700.026.7994694
AGTACAT92900.024.593812
GAGTACA101150.024.412951
GAGTACT47500.023.6799312-13
ATGGGAT37400.022.431475
CAGAGTA117750.022.4126910-11
Produced by FastQC (version 0.11.5)