##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364579_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2343571 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.04552667702408 31.0 28.0 33.0 16.0 34.0 2 27.94862924997792 31.0 28.0 33.0 16.0 34.0 3 28.141736264871003 31.0 28.0 34.0 16.0 34.0 4 31.44826079517113 35.0 32.0 37.0 17.0 37.0 5 31.555429299987072 35.0 32.0 37.0 17.0 37.0 6 31.649106854454164 35.0 32.0 37.0 17.0 37.0 7 31.36356568672338 35.0 32.0 37.0 17.0 37.0 8 31.322153670616338 35.0 32.0 37.0 16.0 37.0 9 32.369684554041676 37.0 32.0 39.0 11.0 39.0 10-11 32.277475485061046 37.0 32.0 39.0 10.0 39.0 12-13 32.20772999836574 37.0 32.0 39.0 10.0 39.0 14-15 32.89084008976046 38.0 31.5 40.0 10.0 41.0 16-17 33.006845962849 38.0 32.0 40.0 10.0 41.0 18-19 32.814098655427976 38.0 31.0 40.0 10.0 41.0 20-21 32.55368431338329 38.0 31.0 40.0 8.0 41.0 22-23 32.453938882158894 38.0 31.0 40.0 8.0 41.0 24-25 32.2842862452215 38.0 30.0 40.0 8.0 41.0 26-27 32.20900732258592 38.0 30.0 40.0 8.0 41.0 28-29 32.27398807204902 38.0 30.5 40.0 7.5 41.0 30-31 32.2230126588868 38.0 30.0 40.0 4.0 41.0 32-33 32.20963158359615 38.0 30.0 40.0 2.0 41.0 34-35 31.726078279685147 37.0 30.0 40.0 2.0 41.0 36-37 31.582405440244823 37.0 30.0 40.0 2.0 41.0 38-39 31.375043469986615 37.0 30.0 40.0 2.0 41.0 40-41 31.592435646285093 37.0 30.0 40.0 2.0 41.0 42-43 31.468145833857818 37.0 30.0 40.0 2.0 41.0 44-45 31.304427303461257 37.0 30.0 40.0 2.0 41.0 46-47 31.262409374411952 37.0 30.0 40.0 2.0 41.0 48-49 31.105175392595317 37.0 29.5 40.0 2.0 41.0 50-51 30.950700661511853 37.0 29.0 40.0 2.0 41.0 52-53 30.898554812292865 36.5 29.0 40.0 2.0 41.0 54-55 30.92964070642622 37.0 29.0 40.0 2.0 41.0 56-57 30.808224286782863 36.5 29.0 40.0 2.0 41.0 58-59 30.61251782002764 36.0 29.0 40.0 2.0 41.0 60-61 30.417974108742598 36.0 28.5 40.0 2.0 41.0 62-63 30.138707553558227 35.0 28.0 39.0 2.0 41.0 64-65 29.8668525510855 35.0 28.0 39.0 2.0 41.0 66-67 29.622189812043246 35.0 28.0 39.0 2.0 40.5 68-69 29.578819459704867 35.0 28.0 39.0 2.0 40.0 70-71 29.35603529826918 35.0 28.0 38.0 2.0 40.0 72-73 29.074923908855332 35.0 28.0 37.5 2.0 40.0 74-75 28.769672862482082 34.0 28.0 37.0 2.0 39.0 76-77 28.46180806982165 34.0 27.5 36.5 2.0 39.0 78-79 28.151831115848424 34.0 27.0 36.0 2.0 39.0 80-81 27.8442195691959 34.0 26.5 36.0 2.0 37.5 82-83 27.58512010090584 34.0 26.0 35.0 2.0 37.0 84-85 27.317173236910683 34.0 26.0 35.0 2.0 37.0 86-87 27.11343586347501 34.0 26.0 35.0 2.0 36.0 88-89 26.904550790225684 34.0 26.0 35.0 2.0 36.0 90-91 26.75599309771285 34.0 25.5 35.0 2.0 36.0 92-93 26.62700084614462 33.5 25.0 35.0 2.0 35.0 94-95 26.4871621128611 33.0 25.0 35.0 2.0 35.0 96-97 26.364543468066465 33.0 25.0 35.0 2.0 35.0 98-99 26.265949271432355 33.0 25.0 35.0 2.0 35.0 100-101 26.16879134449095 33.0 24.0 35.0 2.0 35.0 102-103 25.017559314396706 31.5 21.5 34.0 2.0 35.0 104-105 25.871496532428502 33.0 23.5 35.0 2.0 35.0 106-107 26.013685951908435 33.0 24.0 35.0 2.0 35.0 108-109 25.965979695089246 33.0 24.0 35.0 2.0 35.0 110-111 25.8740801964182 33.0 23.0 35.0 2.0 35.0 112-113 25.772060884863315 33.0 23.0 35.0 2.0 35.0 114-115 25.64765415684014 33.0 21.0 35.0 2.0 35.0 116-117 25.533470716270173 33.0 19.5 35.0 2.0 35.0 118-119 25.379425884686235 33.0 18.5 35.0 2.0 35.0 120-121 25.19664925875939 33.0 16.5 35.0 2.0 35.0 122-123 24.951309988048155 33.0 2.0 35.0 2.0 35.0 124-125 23.517525178456296 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6424685851903043 1101 2 0.7304300680415814 1101 3 0.6924379553084137 1101 4 0.7309227780826468 1101 5 0.6673468726195111 1101 6 0.7781903811961577 1101 7 0.7284998938280367 1101 8 0.7109791891185644 1101 9 0.9441304945508264 1101 10-11 1.0459083882212852 1101 12-13 0.7618951690433278 1101 14-15 1.1239480167986073 1101 16-17 0.7997364669236049 1101 18-19 1.2205589085229462 1101 20-21 1.5541874100072164 1101 22-23 1.3688107844485984 1101 24-25 1.533527969703023 1101 26-27 1.730685184846422 1101 28-29 1.4367674902867549 1101 30-31 1.5363346073458786 1101 32-33 1.4830287645279085 1101 34-35 1.76349026348759 1101 36-37 1.712833768438152 1101 38-39 1.586033745345329 1101 40-41 1.3726609458514183 1101 42-43 1.4283670333581355 1101 44-45 1.268021236711494 1101 46-47 1.1043329770379913 1101 48-49 0.9819653723008592 1101 50-51 1.0146132788547924 1101 52-53 0.829638428147863 1101 54-55 0.8322104483364932 1101 56-57 0.8658092397562314 1101 58-59 0.8657446819284367 1101 60-61 0.8819300920618005 1101 62-63 0.894999483747327 1101 64-65 0.7785050712254709 1101 66-67 0.8185121849579495 1101 68-69 0.7017539924424376 1101 70-71 0.6173390547728843 1101 72-73 0.44712524455968605 1101 74-75 0.4924194893799694 1101 76-77 0.5679171574802453 1101 78-79 0.6450517797141018 1101 80-81 0.7279489909324219 1101 82-83 0.5679275772590806 1101 84-85 0.6543150151330401 1101 86-87 0.7258433599128722 1101 88-89 0.6172996161286122 1101 90-91 0.5635385425817852 1101 92-93 0.38453419991676085 1101 94-95 0.529566581212741 1101 96-97 0.42300243493845 1101 98-99 0.43563202237655574 1101 100-101 0.5419690750159027 1101 102-103 0.5975468478487258 1101 104-105 0.6641583736262326 1101 106-107 0.585116245561121 1101 108-109 0.5813562283152258 1101 110-111 0.48262256442778195 1101 112-113 0.49582025772124894 1101 114-115 0.6543625892538429 1101 116-117 0.7815876466194247 1101 118-119 0.7091061511434837 1101 120-121 0.6996117464296816 1101 122-123 0.8134725142181694 1101 124-125 1.1266317885902168 1102 1 0.07847565456848571 1102 2 0.2789963791259247 1102 3 0.42087747461183156 1102 4 0.43708952928597355 1102 5 0.569405538419673 1102 6 -0.2773350141448141 1102 7 -0.7187931498615647 1102 8 0.165466434650007 1102 9 0.5274466563751261 1102 10-11 0.5689655970849543 1102 12-13 0.6838750968701532 1102 14-15 0.8844744820566817 1102 16-17 0.8263098724829447 1102 18-19 0.9831553661727241 1102 20-21 1.305791294995089 1102 22-23 1.0354919808536778 1102 24-25 1.2111371805004651 1102 26-27 0.6566026717828777 1102 28-29 1.3449562197007623 1102 30-31 1.1221997475974774 1102 32-33 0.9703733623711628 1102 34-35 1.290488003452829 1102 36-37 1.4960705450553107 1102 38-39 1.4462870515212565 1102 40-41 1.276047101388297 1102 42-43 1.1474553451081988 1102 44-45 1.2563487383361895 1102 46-47 0.8615496339301103 1102 48-49 0.642719756319206 1102 50-51 0.8856157845742274 1102 52-53 0.7668785439630845 1102 54-55 0.5712249754331324 1102 56-57 0.5824800011673616 1102 58-59 0.822097677960496 1102 60-61 0.7032499734991333 1102 62-63 0.7056755062446456 1102 64-65 0.7271208164425431 1102 66-67 0.713773740062642 1102 68-69 0.6067656624641558 1102 70-71 0.5232578427813586 1102 72-73 0.629865434344488 1102 74-75 0.2847979481181575 1102 76-77 0.5553367155648594 1102 78-79 0.4938853719409586 1102 80-81 0.10830039265591651 1102 82-83 0.3512924797513257 1102 84-85 -0.05116751747961601 1102 86-87 0.5034688506217648 1102 88-89 0.7317657208906709 1102 90-91 0.06785453533276709 1102 92-93 0.4652393017041838 1102 94-95 0.38637914626634284 1102 96-97 0.5509062361821968 1102 98-99 0.6731004532176463 1102 100-101 0.5999351361887406 1102 102-103 0.40992829839213485 1102 104-105 0.6895632910180645 1102 106-107 0.5669775182605328 1102 108-109 0.1782576955546311 1102 110-111 0.38647449714716586 1102 112-113 0.04156334156576236 1102 114-115 0.11387514294782974 1102 116-117 0.0547257597083437 1102 118-119 0.4412380402208207 1102 120-121 0.8696493124079048 1102 122-123 0.6631666632246009 1102 124-125 0.8918870232586968 1103 1 0.53551061140546 1103 2 0.536068495285015 1103 3 0.3256727023801993 1103 4 0.47387175873700116 1103 5 0.5455197596780579 1103 6 0.5352941804928726 1103 7 0.5887240151732058 1103 8 0.6735806532009683 1103 9 0.9449563455499188 1103 10-11 0.9837929534530048 1103 12-13 0.8597183901201504 1103 14-15 1.1622716347997653 1103 16-17 1.0135196287340378 1103 18-19 1.0309866983851776 1103 20-21 1.416822452646727 1103 22-23 1.4318025813456217 1103 24-25 1.4552692734299129 1103 26-27 1.3930371502189587 1103 28-29 1.3277143196207533 1103 30-31 1.27313436546563 1103 32-33 1.265238505844053 1103 34-35 1.6394368396688819 1103 36-37 1.6504352086382283 1103 38-39 1.6713169515768946 1103 40-41 1.4314303466628395 1103 42-43 1.3030050966348483 1103 44-45 1.0387028575384143 1103 46-47 0.9665423171309797 1103 48-49 1.0069467679651716 1103 50-51 1.0115890593054822 1103 52-53 0.9972221763172833 1103 54-55 0.8465169197794324 1103 56-57 0.8475938278194626 1103 58-59 0.8040653385205765 1103 60-61 0.7470326571196537 1103 62-63 0.7668787795815817 1103 64-65 0.93455998243574 1103 66-67 0.9756021563851291 1103 68-69 1.050455963881081 1103 70-71 1.0057856428953436 1103 72-73 1.0211000477453993 1103 74-75 1.012691408822004 1103 76-77 1.072157823548899 1103 78-79 0.9964050481066025 1103 80-81 1.0128248362475212 1103 82-83 1.031409941113079 1103 84-85 0.9834235641477029 1103 86-87 0.9467882356722086 1103 88-89 0.9308112119555076 1103 90-91 1.0144133045943562 1103 92-93 1.1210374588996217 1103 94-95 0.9527982617932516 1103 96-97 1.0811087189528585 1103 98-99 1.0352030718330418 1103 100-101 1.0058646031927552 1103 102-103 0.9245796640818327 1103 104-105 0.9206992663859701 1103 106-107 1.0537760599094348 1103 108-109 1.0847678192701302 1103 110-111 1.0730645929356406 1103 112-113 1.1445005440035239 1103 114-115 1.2569220347634378 1103 116-117 1.1730740972178708 1103 118-119 1.166817588582763 1103 120-121 1.1766049418759934 1103 122-123 1.181728979881587 1103 124-125 1.2738035474474572 1104 1 0.6755088192946417 1104 2 0.6883371370211648 1104 3 0.7237951660088768 1104 4 0.7845702039006959 1104 5 0.7803790057086886 1104 6 0.8304930291217296 1104 7 0.8071568599040972 1104 8 0.7444031431828044 1104 9 0.9394925696108558 1104 10-11 0.8491356441993005 1104 12-13 0.8529680659907299 1104 14-15 0.8842478621854823 1104 16-17 1.022991631086363 1104 18-19 1.2562708836798322 1104 20-21 1.6245556030214061 1104 22-23 1.3280466918232463 1104 24-25 1.8470495625651253 1104 26-27 1.8419373683231726 1104 28-29 1.7284975633365391 1104 30-31 1.6414722355299034 1104 32-33 1.6367522076843812 1104 34-35 1.9973709779146205 1104 36-37 2.026192801867033 1104 38-39 1.968810110446075 1104 40-41 1.6753470793325214 1104 42-43 1.6519660687372522 1104 44-45 1.559888201915573 1104 46-47 1.5126898914513376 1104 48-49 1.5070972716419924 1104 50-51 1.2341533680972283 1104 52-53 1.457297862970787 1104 54-55 1.2688336001752063 1104 56-57 1.2068965525726227 1104 58-59 1.2564132078905956 1104 60-61 1.228807778921933 1104 62-63 1.3313813162832133 1104 64-65 1.429674678729409 1104 66-67 1.3618675007334566 1104 68-69 1.3811681549671775 1104 70-71 1.4102990475719785 1104 72-73 1.3930413577976282 1104 74-75 1.3706500504596555 1104 76-77 1.4418421790220108 1104 78-79 1.4472386318790171 1104 80-81 1.4146987211329893 1104 82-83 1.3592867053873228 1104 84-85 1.3265689444535589 1104 86-87 1.3645738305124695 1104 88-89 1.4455115655010218 1104 90-91 1.4028953651086056 1104 92-93 1.4060048201413586 1104 94-95 1.4713667880511565 1104 96-97 1.2139376427566795 1104 98-99 1.28886218496881 1104 100-101 1.3858075919675557 1104 102-103 1.3887951027081584 1104 104-105 1.449373880319417 1104 106-107 1.4291312690062448 1104 108-109 1.486261421122201 1104 110-111 1.512861080254293 1104 112-113 1.5355687933971254 1104 114-115 1.4962506274111256 1104 116-117 1.5834220718083323 1104 118-119 1.548927445936986 1104 120-121 1.4723674886336298 1104 122-123 1.5093229942314466 1104 124-125 1.7903039393816087 1105 1 0.36264493398023134 1105 2 0.5631448139272912 1105 3 0.546934015121181 1105 4 0.8131493784636383 1105 5 0.6075112232412252 1105 6 0.6419680545788786 1105 7 0.7432601468293711 1105 8 0.623687300832902 1105 9 0.5979940821650018 1105 10-11 0.8216797390612314 1105 12-13 0.7031459391644646 1105 14-15 0.8821151533987148 1105 16-17 0.8485916443013011 1105 18-19 0.9004327440795521 1105 20-21 1.3731861388987312 1105 22-23 1.5323679362316582 1105 24-25 1.6637366192179783 1105 26-27 1.3848633974929498 1105 28-29 1.2020193911122803 1105 30-31 1.0768750448151536 1105 32-33 1.2380163398550152 1105 34-35 1.7435007362010992 1105 36-37 1.8460136690469895 1105 38-39 1.9899119259572906 1105 40-41 1.4916390188409956 1105 42-43 1.1073745507366368 1105 44-45 1.3959733417922884 1105 46-47 1.2671933618199347 1105 48-49 1.2257190244417693 1105 50-51 1.1416820211360665 1105 52-53 1.1582581064512603 1105 54-55 0.9373426071942319 1105 56-57 0.9212088902091828 1105 58-59 0.9574801964129414 1105 60-61 0.9671774818998422 1105 62-63 0.931821041659969 1105 64-65 0.9726056008695672 1105 66-67 0.734098930873543 1105 68-69 0.6149505220641238 1105 70-71 0.756963768010749 1105 72-73 0.8391556621264513 1105 74-75 0.8465243401080009 1105 76-77 0.9171017941589668 1105 78-79 0.9148926529964498 1105 80-81 0.8748945636425987 1105 82-83 0.8774107765547576 1105 84-85 0.7968664221782156 1105 86-87 0.6645803596921454 1105 88-89 0.8753031554948407 1105 90-91 0.8328753931778152 1105 92-93 0.7850820638598996 1105 94-95 0.8834622656391247 1105 96-97 0.9230737265969857 1105 98-99 0.7237239883047479 1105 100-101 0.8028340093886612 1105 102-103 0.728784167506511 1105 104-105 0.8576670501946602 1105 106-107 0.7987066382474062 1105 108-109 0.7871922911228673 1105 110-111 0.846148654528605 1105 112-113 0.8038611240300853 1105 114-115 0.8080454182687369 1105 116-117 0.8431294314699187 1105 118-119 0.9405724602311132 1105 120-121 0.7325559642870303 1105 122-123 0.8932080315045283 1105 124-125 1.0458181017125199 1106 1 0.27530818687150926 1106 2 0.5816053537848447 1106 3 0.37552748898358246 1106 4 0.49564164193378346 1106 5 0.18712632866559886 1106 6 0.32391181905594735 1106 7 0.15283308092874748 1106 8 0.3441258006885519 1106 9 0.5114839828278406 1106 10-11 0.7171596441708274 1106 12-13 0.6491253659227567 1106 14-15 0.9729043011062757 1106 16-17 0.7808513884690171 1106 18-19 0.6624091128555207 1106 20-21 1.124059715906995 1106 22-23 1.1521886165263737 1106 24-25 1.2700751580270548 1106 26-27 1.3652942405767874 1106 28-29 1.2635570709765034 1106 30-31 1.0556188379657243 1106 32-33 1.0418138465989983 1106 34-35 1.3329020152443505 1106 36-37 1.1960068748436115 1106 38-39 1.390058483816933 1106 40-41 1.0548531930065614 1106 42-43 1.0402394713389924 1106 44-45 0.9236710048620331 1106 46-47 0.715363830549073 1106 48-49 0.8124073879210201 1106 50-51 0.8369556581696393 1106 52-53 0.7919734643836769 1106 54-55 0.5708993549410195 1106 56-57 0.5821745178419349 1106 58-59 0.5668814833335283 1106 60-61 0.6537071958067671 1106 62-63 0.6497806222547808 1106 64-65 0.6709161877351306 1106 66-67 0.624649355875583 1106 68-69 0.729468730332254 1106 70-71 0.7356679775416168 1106 72-73 0.8098099025561751 1106 74-75 0.8387071069360594 1106 76-77 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-2.533868461215423 2216 84-85 -2.5640290775790966 2216 86-87 -2.615685897819624 2216 88-89 -2.6846694798243043 2216 90-91 -2.652507839415051 2216 92-93 -2.641635072334413 2216 94-95 -2.710775904074133 2216 96-97 -2.660052191594435 2216 98-99 -2.7958210122867904 2216 100-101 -2.906496093115596 2216 102-103 -2.672678839844103 2216 104-105 -2.866907712293237 2216 106-107 -2.824031819047004 2216 108-109 -2.9025889636683893 2216 110-111 -2.9706162678555437 2216 112-113 -2.912193535024599 2216 114-115 -3.0504966314041617 2216 116-117 -3.078164498774779 2216 118-119 -3.065996905557146 2216 120-121 -3.055142473061643 2216 122-123 -3.162733702887948 2216 124-125 -3.195702348455754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 162746.0 3 26985.0 4 16711.0 5 13122.0 6 12315.0 7 13178.0 8 13553.0 9 14315.0 10 14956.0 11 16529.0 12 17765.0 13 19678.0 14 22257.0 15 19990.0 16 15801.0 17 13754.0 18 12648.0 19 11816.0 20 11689.0 21 12426.0 22 14813.0 23 19319.0 24 25856.0 25 32993.0 26 40403.0 27 45218.0 28 51888.0 29 60795.0 30 68866.0 31 78916.0 32 95912.0 33 119241.0 34 153536.0 35 207689.0 36 313022.0 37 411717.0 38 140795.0 39 358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3096991039864 19.356514064972508 13.173439828478859 23.160347002562233 2 14.854210672299386 20.73553948238708 41.19494761996202 23.21530222535151 3 19.267826189372776 26.44242627523174 28.70744711267239 25.582300422723094 4 11.824757401701817 17.001382618269336 38.689201068542026 32.484658911486825 5 12.988301587897698 39.13522565490965 34.272395639512055 13.604077117680596 6 30.427126061478603 37.67170020486041 18.42877484428518 13.472398889375805 7 28.040922296455356 32.99430563189392 21.266996903601996 17.697775168048725 8 25.732439938879597 35.98777250614554 20.14374644506183 18.136041109913034 9 26.884287127922818 16.657570581425464 20.13382553182229 36.324316758829426 10-11 24.468514075314978 27.58463473050315 27.765299195117194 20.181551999064677 12-13 25.198476113098423 25.137242418436507 27.927856039797206 21.736425428667864 14-15 22.523490288063314 26.348088170523106 25.90352678678125 25.22489475463233 16-17 21.92790734352781 29.672919784813455 26.385428233555125 22.013744638103606 18-19 21.70800821276375 28.502847647599637 28.586272531386214 21.2028716082504 20-21 22.74385924484501 27.520483408439166 28.602412774819065 21.133244571896764 22-23 22.49069775380624 27.494175428415375 28.159072676998704 21.856054140779683 24-25 21.942561634838732 27.711192488126667 28.551895486534455 21.794350390500142 26-27 21.88222400191541 27.871979883065194 28.409920957295686 21.83587515772371 28-29 21.729592723882387 27.649502945841952 28.69451677631123 21.92638755396443 30-31 21.914191728962372 27.6340106173302 28.501911861907285 21.949885791800135 32-33 21.905281103375287 27.658570724452762 28.233088883246456 22.203059288925495 34-35 22.166008596694677 27.58909549073599 28.55429797394107 21.69059793862826 36-37 22.26978356707526 27.30844823057501 28.38247258821413 22.039295614135597 38-39 21.942667834684762 27.69901146583568 28.40991375981355 21.948406939666004 40-41 22.228856783115305 27.548197784843893 28.349730041187392 21.873215390853414 42-43 22.055059715272527 27.533036874684875 28.15399121377989 22.25791219626271 44-45 22.12000466892758 27.746638553520143 28.026048689574147 22.10730808797813 46-47 22.296634873980327 27.985394267928644 27.518371845774425 22.199599012316597 48-49 22.106329059897458 27.867752898307163 27.67242326755123 22.353494774244144 50-51 22.110258026868422 28.046401524243556 27.463864823663325 22.379475625224696 52-53 22.415066580018273 28.021489427885903 27.377813601550795 22.18563039054503 54-55 22.315559012473713 27.84468621532546 27.535654803249077 22.304099968951753 56-57 22.19268126209621 28.249369892814038 27.340432409389486 22.21751643570026 58-59 22.210917038393628 27.805276232886122 27.540487445201034 22.443319283519216 60-61 22.324755841685302 27.972753097024526 27.618127733406045 22.084363327884123 62-63 22.172393379341663 27.84945979549355 27.71836208747331 22.259784737691476 64-65 22.10819691231164 27.73836611442444 27.579349032891482 22.574087940372436 66-67 22.08217890942731 28.053130311185605 27.348250773088868 22.516440006298215 68-69 22.188367926813264 27.74399580034122 27.671163303347456 22.396472969498056 70-71 22.017963798452822 27.75965429353046 27.71836973131111 22.504012176705608 72-73 22.21992249295963 27.875179610040835 27.3880400517055 22.516857845294037 74-75 22.066415326528475 28.021282056588444 27.412211409897846 22.50009120698524 76-77 22.159891508713354 27.79444861215304 27.572955525570812 22.472704353562794 78-79 22.18366480033573 27.891957765719606 27.407883735185113 22.51649369875955 80-81 22.016317613122364 27.945939372215676 27.47326625360575 22.564476761056206 82-83 22.20285207052366 28.099288870549948 27.307511695520276 22.390347363406114 84-85 22.206111954790362 27.934442779843238 27.34177884945666 22.517666415909737 86-87 22.0750990377121 27.84959453586214 27.619077286647258 22.4562291397785 88-89 22.28156564201642 27.701661999854043 27.51447736179769 22.502294996331848 90-91 22.13517431407626 27.97661486116354 27.50310335798592 22.385107466774276 92-93 22.151704008414963 27.934771244898005 27.490510765134236 22.4230139815528 94-95 22.085848329410922 27.72722888192899 27.46633938639644 22.720583402263646 96-97 22.26038103281869 27.62471931259513 27.493356646863397 22.621543007722785 98-99 21.939749525292825 27.668153015724005 27.417614303087195 22.97448315589597 100-101 21.869665102029973 27.725093354380075 27.610748774567067 22.79449276902288 102-103 22.034536184310184 27.505247334089727 27.59933451984173 22.86088196175836 104-105 21.90298457308821 27.512396339802127 27.687793800347293 22.89682528676237 106-107 21.820275750479613 27.598665795061205 27.671716801048657 22.909341653410525 108-109 21.85875742616716 27.73459818371195 27.23881631919835 23.167828070922535 110-111 21.60190446280597 27.705897212261064 27.571378951913932 23.12081937301903 112-113 21.797440743207694 27.646463452568753 27.70172100610564 22.854374798117917 114-115 21.61711763799774 27.982126421601905 27.46174534503115 22.939010595369204 116-117 21.525291958297828 27.919636315690884 27.53221472701275 23.022856998998538 118-119 21.50905605164085 27.789322363179952 27.578319581527506 23.12330200365169 120-121 21.343647518730517 27.798206026408018 27.656243032793792 23.201903422067673 122-123 21.48328341663214 27.820172719324482 27.63485723282973 23.06168663121365 124-125 21.427554734206065 27.724621243241916 27.574516304226048 23.27330771832597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 905.0 1 894.0 2 1002.0 3 975.5 4 713.5 5 573.5 6 542.5 7 611.0 8 813.0 9 935.5 10 906.0 11 932.0 12 1050.0 13 1183.0 14 1323.5 15 1454.5 16 1593.5 17 1726.0 18 1970.0 19 2361.0 20 2793.0 21 3403.5 22 4367.5 23 5511.0 24 6904.5 25 9215.5 26 12778.5 27 16578.5 28 19994.0 29 24056.5 30 29496.0 31 35668.5 32 42270.5 33 49149.0 34 56580.0 35 62975.5 36 69569.0 37 75363.0 38 77484.0 39 79041.5 40 79288.5 41 79882.5 42 80539.5 43 80848.5 44 81888.5 45 82806.5 46 82960.5 47 81367.0 48 81676.5 49 81520.0 50 79106.0 51 76397.0 52 73581.5 53 71903.0 54 69628.5 55 64788.0 56 59786.5 57 55598.5 58 51669.5 59 46659.5 60 39640.0 61 32226.5 62 25565.0 63 19710.5 64 14568.5 65 10752.5 66 8113.5 67 6263.0 68 4744.0 69 3463.0 70 2537.5 71 2026.0 72 1619.5 73 1307.5 74 1047.0 75 786.0 76 588.5 77 406.0 78 282.0 79 189.5 80 132.0 81 112.0 82 86.5 83 59.5 84 53.0 85 43.0 86 28.5 87 19.0 88 15.0 89 11.0 90 10.5 91 10.0 92 11.0 93 8.5 94 3.0 95 3.5 96 5.5 97 5.0 98 2.5 99 1.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012928987429866643 2 0.005163060986844435 3 0.00823529562364443 4 0.008149955772622208 5 0.014806464152355531 6 0.0010240782122666648 7 0.00866199487875554 8 0.0 9 0.0030722346367999943 10-11 0.0 12-13 0.0038402932959999934 14-15 0.020268214617777742 16-17 0.03270649790426661 18-19 0.006208474161866656 20-21 0.009003354282844428 22-23 0.0021761662010666627 24-25 0.012544958100266644 26-27 0.02007619995297774 28-29 5.76043994399999E-4 30-31 0.0018774767224888854 32-33 0.009216703910399984 34-35 0.009750077979288872 36-37 0.012011584031377757 38-39 0.0 40-41 0.026498023742399953 42-43 0.010347456936444425 44-45 0.01794270367742219 46-47 0.004587016992444436 48-49 0.025964649673511066 50-51 0.004843036545511103 52-53 0.0 54-55 0.01892411196417774 56-57 0.0051417260240888794 58-59 2.1334962755555518E-4 60-61 0.004779031657244436 62-63 0.028247490688355508 64-65 0.018198723230488857 66-67 0.002133496275555552 68-69 0.022337706005066627 70-71 0.003413594040888883 72-73 0.012800977653333311 74-75 2.1334962755555518E-5 76-77 0.006699178305244432 78-79 0.0010454131750222205 80-81 0.0038829632215111043 82-83 0.0019414816107555521 84-85 0.0 86-87 0.006208474161866656 88-89 0.018348067969777743 90-91 0.023233774440799958 92-93 0.00537641061439999 94-95 0.026668703444444396 96-97 0.018753432262133298 98-99 8.960684357333317E-4 100-101 0.009195368947644429 102-103 0.0 104-105 0.001557452281155553 106-107 1.280097765333331E-4 108-109 0.0 110-111 9.814082867555538E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.0667481377777759E-4 122-123 0.0 124-125 0.023191104515288847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2343571.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.284896774868386 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25726103240514 42.7115970128558 2 11.611824163630342 12.839170005084515 3 4.44005495550548 7.364039396695898 4 2.17675246305904 4.813661408986381 5 1.2240637848218499 3.3836119994865332 6 0.7656840384483152 2.5398457816667674 7 0.5005115690204274 1.9369511299545215 8 0.3530675085714753 1.5615440612747173 9 0.2664229060375065 1.3256246572869608 >10 1.2629639085883213 12.468930177662061 >50 0.09228168418064983 3.520199266237504 >100 0.04753446269389154 4.7291931835391905 >500 0.001262018430129205 0.4316181183722961 >1k 3.1550460753221E-4 0.37401380089697595 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3578 0.1526729934787553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2407 0.10270651070524427 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.1334962755555518E-5 0.0 14-15 0.0 0.0 0.0 8.533985102222207E-5 0.0 16-17 0.0 0.0 0.0 1.280097765333331E-4 0.0 18-19 0.0 0.0 0.0 1.7067970204444414E-4 0.0 20-21 0.0 0.0 0.0 2.3468459031111068E-4 0.0 22-23 0.0 0.0 0.0 3.413594040888883E-4 0.0 24-25 0.0 0.0 0.0 4.907041433777769E-4 0.0 26-27 0.0 0.0 0.0 7.893936219555541E-4 0.0 28-29 2.1334962755555518E-5 0.0 0.0 0.0016001222066666636 0.0 30-31 4.2669925511111035E-5 0.0 0.0 0.0035202688546666604 0.0 32-33 4.2669925511111035E-5 0.0 0.0 0.007787261405777764 0.0 34-35 4.2669925511111035E-5 0.0 0.0 0.013953065642133309 0.0 36-37 4.2669925511111035E-5 0.0 0.0 0.025537950418399954 0.0 38-39 4.2669925511111035E-5 0.0 0.0 0.045294125930044365 0.0 40-41 4.2669925511111035E-5 0.0 0.0 0.07000001280097765 0.0 42-43 4.2669925511111035E-5 0.0 0.0 0.09289242783768872 0.0 44-45 4.2669925511111035E-5 0.0 0.0 0.12020118016479978 0.0 46-47 4.2669925511111035E-5 0.0 0.0 0.15382508146755527 0.0 48-49 4.2669925511111035E-5 0.0 0.0 0.19137461591733299 0.0 50-51 4.2669925511111035E-5 0.0 0.0 0.23494060986417736 0.0 52-53 4.2669925511111035E-5 0.0 0.0 0.27528502443493286 0.0 54-55 4.2669925511111035E-5 0.0 0.0 0.3216672334655105 0.0 56-57 4.2669925511111035E-5 0.0 0.0 0.3772234764809771 0.0 58-59 4.2669925511111035E-5 0.0 0.0 0.44325518620942145 4.2669925511111035E-5 60-61 4.2669925511111035E-5 0.0 0.0 0.5171808321574213 4.2669925511111035E-5 62-63 4.2669925511111035E-5 0.0 0.0 0.5912771578074656 4.2669925511111035E-5 64-65 4.2669925511111035E-5 0.0 0.0 0.6657148428615989 4.2669925511111035E-5 66-67 4.2669925511111035E-5 0.0 0.0 0.7558337255410653 4.2669925511111035E-5 68-69 4.2669925511111035E-5 0.0 0.0 0.8609937569631985 4.2669925511111035E-5 70-71 4.2669925511111035E-5 0.0 0.0 0.9823896950423093 4.2669925511111035E-5 72-73 4.2669925511111035E-5 0.0 0.0 1.1056417748811536 4.2669925511111035E-5 74-75 4.2669925511111035E-5 0.0 0.0 1.2276137569546646 4.2669925511111035E-5 76-77 4.2669925511111035E-5 0.0 0.0 1.3627920809738643 4.2669925511111035E-5 78-79 4.2669925511111035E-5 0.0 0.0 1.5206068004767084 4.2669925511111035E-5 80-81 4.2669925511111035E-5 0.0 0.0 1.6990310940014193 4.2669925511111035E-5 82-83 4.2669925511111035E-5 0.0 0.0 1.8894456365947523 4.2669925511111035E-5 84-85 4.2669925511111035E-5 0.0 0.0 2.0796041596350188 4.2669925511111035E-5 86-87 4.2669925511111035E-5 0.0 0.0 2.278766036958129 4.2669925511111035E-5 88-89 4.2669925511111035E-5 0.0 0.0 2.5105917422599955 4.2669925511111035E-5 90-91 4.2669925511111035E-5 0.0 0.0 2.7777481458850617 4.2669925511111035E-5 92-93 8.533985102222207E-5 0.0 0.0 3.0644473753942165 4.2669925511111035E-5 94-95 8.533985102222207E-5 4.2669925511111035E-5 0.0 3.349546482696705 4.2669925511111035E-5 96-97 8.533985102222207E-5 4.2669925511111035E-5 0.0 3.6439476337606154 4.2669925511111035E-5 98-99 8.533985102222207E-5 4.2669925511111035E-5 0.0 3.9645054491628375 4.2669925511111035E-5 100-101 8.533985102222207E-5 4.2669925511111035E-5 0.0 4.346614632114837 4.2669925511111035E-5 102-103 8.533985102222207E-5 4.2669925511111035E-5 0.0 4.7488213499825696 4.2669925511111035E-5 104-105 8.533985102222207E-5 4.2669925511111035E-5 0.0 5.150238674228346 4.2669925511111035E-5 106-107 8.533985102222207E-5 4.2669925511111035E-5 0.0 5.5538961695634566 4.2669925511111035E-5 108-109 8.533985102222207E-5 4.2669925511111035E-5 0.0 5.9774165152239895 4.2669925511111035E-5 110-111 8.533985102222207E-5 4.2669925511111035E-5 0.0 6.457218492633677 4.2669925511111035E-5 112-113 8.533985102222207E-5 6.400488826666655E-5 0.0 6.978132943273321 4.2669925511111035E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7650 0.0 47.991222 1 GGTATCA 4865 0.0 44.275536 1 TCAACGC 9010 0.0 39.555992 4 ATCAACG 9090 0.0 39.46969 3 CAACGCA 9090 0.0 38.946045 5 AACGCAG 9270 0.0 38.440807 6 TATCAAC 9570 0.0 37.55219 2 GTACATG 12575 0.0 35.441483 1 TACATGG 12960 0.0 33.60596 2 ACATGGG 12990 0.0 32.703884 3 ACGCAGA 11065 0.0 31.774702 7 CGCAGAG 11010 0.0 31.23367 8 CATGGGG 4920 0.0 27.935543 4 GCAGAGT 11910 0.0 27.275494 9 CATGGGA 8370 0.0 26.799469 4 AGTACAT 9290 0.0 24.59381 2 GAGTACA 10115 0.0 24.41295 1 GAGTACT 4750 0.0 23.67993 12-13 ATGGGAT 3740 0.0 22.43147 5 CAGAGTA 11775 0.0 22.41269 10-11 >>END_MODULE