FastQCFastQC Report
Thu 26 May 2016
SRR1364579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343571
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.3091436103279994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61480.2623347020423107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38040.16231639664426636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57150.056.7490041
GTATCAA96250.050.8531651
ATCAACG124100.038.8295633
TATCAAC125850.038.1003042
TCAACGC126950.037.8170934
CAACGCA126500.037.534045
AACGCAG130350.036.516736
GTACATG117950.032.39931
ACGCAGA153850.030.8229187
TACATGG120900.030.496512
CGCAGAG155800.030.169818
ACATGGG122350.029.5995673
GCAGAGT166950.027.4058679
TGGTATC27850.027.1621592
GAGTACT98700.026.01216912-13
CGGTATA2809.276846E-1125.5275422
AGAGTAC156350.024.56285510-11
CATGGGG48550.024.5372854
CATGGGA77300.024.1955744
AGTACTT105400.024.16106612-13
Produced by FastQC (version 0.11.5)