##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364579_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2343571 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.11732864077939 31.0 30.0 34.0 26.0 34.0 2 29.968246748231653 31.0 30.0 34.0 26.0 34.0 3 30.23860894336037 31.0 30.0 34.0 26.0 34.0 4 33.814635016391655 35.0 35.0 37.0 30.0 37.0 5 33.27355902594801 35.0 33.0 37.0 27.0 37.0 6 33.3124411421715 36.0 35.0 37.0 27.0 37.0 7 33.10284945495571 35.0 33.0 37.0 26.0 37.0 8 33.02833667083267 36.0 33.0 37.0 26.0 37.0 9 34.24448032511069 38.0 34.0 39.0 25.0 39.0 10-11 34.19245160483723 38.0 34.0 39.0 25.0 39.0 12-13 34.09194686228837 38.0 34.0 39.0 25.0 39.0 14-15 35.04858483058546 39.0 34.0 41.0 25.0 41.0 16-17 35.067221347251696 39.0 34.0 41.0 25.0 41.0 18-19 35.110616234797234 39.0 34.0 41.0 25.0 41.0 20-21 35.131777744305595 39.0 34.0 41.0 25.0 41.0 22-23 35.09203988272598 39.0 34.0 41.0 25.0 41.0 24-25 35.05665883389068 39.0 34.0 41.0 25.0 41.0 26-27 34.93567103364907 39.0 34.0 41.0 25.0 41.0 28-29 34.78286000296129 39.0 34.0 41.0 24.0 41.0 30-31 34.61386533627528 38.5 34.0 41.0 24.0 41.0 32-33 34.39230942864543 38.0 33.0 40.5 23.5 41.0 34-35 34.21038662792806 38.0 33.0 40.0 22.0 41.0 36-37 33.99960380974163 38.0 33.0 40.0 20.0 41.0 38-39 33.826099358628355 38.0 33.0 40.0 18.5 41.0 40-41 33.84827193202169 38.0 33.0 40.0 18.5 41.0 42-43 33.70377044262794 38.0 33.0 40.0 16.5 41.0 44-45 33.52569156215024 38.0 33.0 40.0 15.0 41.0 46-47 33.25299574879532 38.0 32.0 40.0 11.0 41.0 48-49 32.950333060103574 38.0 32.0 40.0 8.0 41.0 50-51 32.78186344685098 38.0 32.0 40.0 6.5 41.0 52-53 32.59987941479051 38.0 31.0 40.0 2.0 41.0 54-55 32.410875966633824 38.0 31.0 40.0 2.0 41.0 56-57 32.20215005220665 37.0 31.0 40.0 2.0 41.0 58-59 32.00225062522108 37.0 31.0 40.0 2.0 41.0 60-61 31.773408187761326 36.5 30.5 40.0 2.0 41.0 62-63 31.60654829744864 36.0 30.5 40.0 2.0 41.0 64-65 31.476821696462366 36.0 30.5 39.5 2.0 41.0 66-67 31.181990005850047 35.5 30.0 39.0 2.0 41.0 68-69 30.894792178261294 35.0 30.0 39.0 2.0 41.0 70-71 30.590007300824254 35.0 30.0 38.5 2.0 40.0 72-73 30.30114705293759 35.0 29.5 37.5 2.0 40.0 74-75 29.944339429016658 35.0 29.0 37.0 2.0 39.5 76-77 29.63216988092104 35.0 29.0 36.5 2.0 39.0 78-79 29.33896583461734 34.5 29.0 36.0 2.0 39.0 80-81 29.049882209670628 34.0 29.0 36.0 2.0 37.5 82-83 28.759143204963706 34.0 29.0 35.0 2.0 37.0 84-85 28.52170277751346 34.0 29.0 35.0 2.0 37.0 86-87 28.303411972583717 34.0 28.5 35.0 2.0 36.0 88-89 28.11610081367281 34.0 28.0 35.0 2.0 36.0 90-91 27.963643303317888 34.0 28.0 35.0 2.0 36.0 92-93 27.84852027098816 34.0 27.5 35.0 2.0 35.0 94-95 27.730754903521166 34.0 27.0 35.0 2.0 35.0 96-97 27.611119313219014 34.0 27.0 35.0 2.0 35.0 98-99 27.529026856877817 34.0 27.0 35.0 2.0 35.0 100-101 27.425370086931437 34.0 27.0 35.0 2.0 35.0 102-103 26.15359338377203 31.5 24.5 34.0 2.0 35.0 104-105 27.09053662125022 33.5 25.5 35.0 2.0 35.0 106-107 27.2552440271705 34.0 27.0 35.0 2.0 35.0 108-109 27.263791026600003 34.0 27.0 35.0 2.0 35.0 110-111 27.18604727571727 34.0 27.0 35.0 2.0 35.0 112-113 27.10174942427603 34.0 26.5 35.0 2.0 35.0 114-115 26.99858719876633 34.0 25.5 35.0 2.0 35.0 116-117 26.875065871697508 34.0 25.0 35.0 2.0 35.0 118-119 26.738902299098257 34.0 25.0 35.0 2.0 35.0 120-121 26.543033473276466 34.0 25.0 35.0 2.0 35.0 122-123 26.2516204970961 34.0 25.0 35.0 2.0 35.0 124-125 24.442541745054875 31.5 13.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5022329188486339 1101 2 0.6686738701558355 1101 3 0.4737769554931006 1101 4 0.4079977253142957 1101 5 0.3909899599603506 1101 6 0.6048238812499491 1101 7 0.5702453076659211 1101 8 0.6941430130884925 1101 9 0.646496175723442 1101 10-11 0.7234412821006089 1101 12-13 0.6593926027627575 1101 14-15 0.7837150115239382 1101 16-17 0.8698454545776713 1101 18-19 0.8945894594556094 1101 20-21 0.9179181625730877 1101 22-23 0.9156831104680236 1101 24-25 0.943359590597133 1101 26-27 0.9041855302128425 1101 28-29 1.021137560829736 1101 30-31 0.8696612619041275 1101 32-33 1.021256028009347 1101 34-35 1.098654420519864 1101 36-37 1.2593299727493559 1101 38-39 1.1108985785891718 1101 40-41 0.9025536486905494 1101 42-43 0.8974128231572251 1101 44-45 0.7610449390662453 1101 46-47 0.8850204192310329 1101 48-49 0.948263064871874 1101 50-51 0.9337261660097127 1101 52-53 0.8073505347469663 1101 54-55 0.9104776676190127 1101 56-57 0.8389778504536949 1101 58-59 0.8758958475185565 1101 60-61 0.8542710235506021 1101 62-63 0.8255060049351286 1101 64-65 0.7130933916592141 1101 66-67 0.7781627973995029 1101 68-69 0.977007468162153 1101 70-71 0.7868793593243417 1101 72-73 0.7820067142428542 1101 74-75 0.7937898665345386 1101 76-77 0.7998563337793811 1101 78-79 0.7900891894643429 1101 80-81 0.7076561445008593 1101 82-83 0.7877681487797688 1101 84-85 0.7952932891516618 1101 86-87 0.843626335479442 1101 88-89 0.764412810849052 1101 90-91 0.7875671069900996 1101 92-93 0.6553421276623475 1101 94-95 0.7112676339741064 1101 96-97 0.691317289276725 1101 98-99 0.6487425794122359 1101 100-101 0.6254976078621901 1101 102-103 0.5822420302495566 1101 104-105 0.6165709979344314 1101 106-107 0.5812086485166965 1101 108-109 0.621115460340885 1101 110-111 0.4987222463065919 1101 112-113 0.5190197765624625 1101 114-115 0.6222535927902975 1101 116-117 0.5906128161022934 1101 118-119 0.6411219153886911 1101 120-121 0.6350155346814006 1101 122-123 0.5845301691001303 1101 124-125 0.9721180099749773 1102 1 0.26739271955345956 1102 2 -0.4716233026212464 1102 3 0.11587779716121815 1102 4 0.2542588933995873 1102 5 0.4657560311719209 1102 6 0.3596484148453456 1102 7 0.32254265744163746 1102 8 0.5439788270444339 1102 9 0.8093022248498087 1102 10-11 0.8131396315223185 1102 12-13 0.7836963310936298 1102 14-15 1.047743969292597 1102 16-17 0.9861505619128081 1102 18-19 1.0946619760591432 1102 20-21 0.9310720338237601 1102 22-23 1.0957541212623028 1102 24-25 1.0562054644155978 1102 26-27 1.0629769135496119 1102 28-29 0.7426708312889332 1102 30-31 0.9061257868873511 1102 32-33 1.1269765511327705 1102 34-35 1.1114191259360169 1102 36-37 1.0205310801796017 1102 38-39 0.4193218311899969 1102 40-41 0.06756045785432008 1102 42-43 0.4533039386184541 1102 44-45 0.15719776153397902 1102 46-47 0.3441547043943203 1102 48-49 -0.026443137213014722 1102 50-51 -0.04549151985206379 1102 52-53 -0.2912968126511615 1102 54-55 -0.19717795248380554 1102 56-57 -0.16091829493878862 1102 58-59 -0.11357421989943006 1102 60-61 -0.29053152390880754 1102 62-63 -0.19953632943906285 1102 64-65 -0.05607859817524741 1102 66-67 -0.3354493550095299 1102 68-69 -0.03440217403643331 1102 70-71 -0.6103082116732423 1102 72-73 -0.39307547784557073 1102 74-75 -0.17382664875867349 1102 76-77 -0.586567012604295 1102 78-79 -0.549352577985875 1102 80-81 -0.48092995344542544 1102 82-83 -0.7149284385809445 1102 84-85 -0.5935045136877122 1102 86-87 -0.38984014309315995 1102 88-89 -0.480374810606353 1102 90-91 -0.40892300354158095 1102 92-93 -0.3114618780544447 1102 94-95 -0.2016449115303942 1102 96-97 -0.46809920303307706 1102 98-99 -0.651492174929512 1102 100-101 -0.45380454861517094 1102 102-103 -0.5047787195377786 1102 104-105 -0.3624802563782552 1102 106-107 -0.2844244584168969 1102 108-109 -0.37518490501599544 1102 110-111 -0.26805646445651377 1102 112-113 -0.09076940353760676 1102 114-115 -0.02269557293854163 1102 116-117 -0.1605013506568369 1102 118-119 -0.010412524054000727 1102 120-121 0.19460215701915473 1102 122-123 0.13351569615978676 1102 124-125 0.44653262662763993 1103 1 0.24220831049497704 1103 2 -0.20715401084137497 1103 3 0.22106846035697458 1103 4 0.13055955428050225 1103 5 0.23179917930427507 1103 6 0.3014712318874615 1103 7 0.37658468131836287 1103 8 0.3636767535677947 1103 9 0.5634580279988057 1103 10-11 0.600301238023377 1103 12-13 0.5411932158699528 1103 14-15 0.6722387395748868 1103 16-17 0.395463075534515 1103 18-19 0.6679556930878547 1103 20-21 0.67863201499366 1103 22-23 0.6604575370771428 1103 24-25 0.5678808692762303 1103 26-27 0.6572095109388485 1103 28-29 0.5795433531547189 1103 30-31 0.6371502927495882 1103 32-33 0.7619384560590561 1103 34-35 0.7922866268937696 1103 36-37 0.9696679889301123 1103 38-39 0.8579507824984276 1103 40-41 0.7439857192217545 1103 42-43 0.7605885617660704 1103 44-45 0.7827305730800376 1103 46-47 0.8548993071550726 1103 48-49 0.7374494320732126 1103 50-51 0.6902378029142184 1103 52-53 0.7787611159569181 1103 54-55 0.806717896163363 1103 56-57 0.8122543581691275 1103 58-59 0.8923652222504757 1103 60-61 0.8654878378578346 1103 62-63 0.849142467140382 1103 64-65 0.8459172432295787 1103 66-67 0.6997246254104184 1103 68-69 0.7277388645429319 1103 70-71 0.6980324316906703 1103 72-73 0.575599729711918 1103 74-75 0.7762967756928383 1103 76-77 0.7976256046709302 1103 78-79 0.6951154886870192 1103 80-81 0.6047926769201695 1103 82-83 0.6785789682398544 1103 84-85 0.6823369097512213 1103 86-87 0.7704256780466423 1103 88-89 0.8075870496475233 1103 90-91 0.7210672345217475 1103 92-93 0.64497994256282 1103 94-95 0.622421900456974 1103 96-97 0.6625107800997014 1103 98-99 0.7894550036694241 1103 100-101 0.6844733520117323 1103 102-103 0.47837610183350066 1103 104-105 0.5347687608287437 1103 106-107 0.6136524366746912 1103 108-109 0.6504313796338899 1103 110-111 0.6373192931130944 1103 112-113 0.5531476272768785 1103 114-115 0.558990253792075 1103 116-117 0.6950981327676686 1103 118-119 0.6294339026947533 1103 120-121 0.6996881700201811 1103 122-123 0.6517847232217875 1103 124-125 0.9264528440739781 1104 1 0.3747286597211641 1104 2 0.5554722627044022 1104 3 0.28567213360760846 1104 4 0.10935811453806821 1104 5 0.3672736286325815 1104 6 0.4328425642161591 1104 7 0.557416590692327 1104 8 0.5142880531388201 1104 9 0.691177634243715 1104 10-11 0.629285147222447 1104 12-13 0.6534935822917518 1104 14-15 0.759020745148689 1104 16-17 0.6521053565216306 1104 18-19 0.6654718419041643 1104 20-21 0.5574956533774085 1104 22-23 0.6945824741070297 1104 24-25 0.7685883927064054 1104 26-27 0.7709320350092312 1104 28-29 0.93629712457507 1104 30-31 0.825606600679393 1104 32-33 0.8353024522643864 1104 34-35 1.0023100036101198 1104 36-37 1.0661342394715305 1104 38-39 1.041338611855842 1104 40-41 1.009365861720994 1104 42-43 0.9168665109474787 1104 44-45 0.7789093652021464 1104 46-47 0.8051534577774291 1104 48-49 0.7775032290299393 1104 50-51 0.6403600676330043 1104 52-53 0.7593420577471832 1104 54-55 0.3305172960224496 1104 56-57 0.6006590408153656 1104 58-59 0.6807653405394021 1104 60-61 0.723188112848355 1104 62-63 0.8167307255954341 1104 64-65 0.6901978272463083 1104 66-67 0.7442669873308034 1104 68-69 0.49320164593255456 1104 70-71 0.11928105247401533 1104 72-73 0.08152030172678337 1104 74-75 0.5449745462397146 1104 76-77 0.7494501344400284 1104 78-79 0.7033552346781313 1104 80-81 0.6291938512572415 1104 82-83 0.6348733250846763 1104 84-85 0.7425519534405289 1104 86-87 0.7781078517569497 1104 88-89 0.7193403658332862 1104 90-91 0.7820886079656901 1104 92-93 0.8082432802755335 1104 94-95 0.7380423563690037 1104 96-97 0.7818457820659788 1104 98-99 0.7331266303511228 1104 100-101 0.6787812380921032 1104 102-103 0.6161694849087205 1104 104-105 0.6158630967255654 1104 106-107 0.6074172843488732 1104 108-109 0.6278336068961217 1104 110-111 0.662484877186273 1104 112-113 0.7247260228764532 1104 114-115 0.7218843590206916 1104 116-117 0.6624023200370033 1104 118-119 0.7165940292379567 1104 120-121 0.7549553302620353 1104 122-123 0.8259694869646523 1104 124-125 1.3233441323734354 1105 1 0.20717495623970805 1105 2 -0.03436905396026546 1105 3 0.15403666882591693 1105 4 0.10070483770014249 1105 5 0.05778730205788918 1105 6 0.3779359209167481 1105 7 0.4623834410136993 1105 8 0.48753939751178876 1105 9 0.5893779371068391 1105 10-11 0.6216675752114327 1105 12-13 0.6374674602494892 1105 14-15 0.6979769078135689 1105 16-17 0.6081235026307468 1105 18-19 0.7113294257773077 1105 20-21 0.661879152026593 1105 22-23 0.6747666094999829 1105 24-25 0.6041239150059354 1105 26-27 0.7141720128162774 1105 28-29 0.7377785516032276 1105 30-31 0.7589712333656706 1105 32-33 0.9009175373938518 1105 34-35 0.8362825937380407 1105 36-37 0.918537905300596 1105 38-39 0.9414747303775215 1105 40-41 0.694930002025707 1105 42-43 0.7248252130248503 1105 44-45 0.7494301580950804 1105 46-47 0.7284640149207817 1105 48-49 0.8392525152629915 1105 50-51 0.6509432494212675 1105 52-53 0.6595569726639141 1105 54-55 0.6797024050773643 1105 56-57 0.6205022669127942 1105 58-59 0.3739382455084481 1105 60-61 0.383120251654951 1105 62-63 0.7308116531335216 1105 64-65 0.4123702716498343 1105 66-67 0.6230472473189792 1105 68-69 0.7405990749858553 1105 70-71 0.7350481054348208 1105 72-73 0.7706844547767062 1105 74-75 0.719646308171729 1105 76-77 0.731365324466946 1105 78-79 0.6847294187557473 1105 80-81 0.7023619994761958 1105 82-83 0.6519238002625585 1105 84-85 0.6291266369612458 1105 86-87 0.805582545901288 1105 88-89 0.7497266513618293 1105 90-91 0.6057704202154497 1105 92-93 0.7237027728611984 1105 94-95 0.7046591851965367 1105 96-97 0.6407183070352005 1105 98-99 0.5225206590640106 1105 100-101 0.6248053160470626 1105 102-103 0.25379739009031255 1105 104-105 0.5438701617050725 1105 106-107 0.5193996756053458 1105 108-109 0.5324496899566817 1105 110-111 0.4054653631592231 1105 112-113 0.4035728977206112 1105 114-115 0.4878295904765899 1105 116-117 0.1900331977959624 1105 118-119 0.3862777405865252 1105 120-121 0.3096711477165037 1105 122-123 0.4412940196840225 1105 124-125 0.9400341952672875 1106 1 -0.040781800329696694 1106 2 -0.4582517225973177 1106 3 0.07653461973356457 1106 4 -0.1917959766195949 1106 5 0.07067785551566175 1106 6 0.2448847800616889 1106 7 0.28199651408505844 1106 8 0.3135085364676229 1106 9 0.3586768028884322 1106 10-11 0.3535472378565885 1106 12-13 0.30909644239116574 1106 14-15 0.43158080142382715 1106 16-17 0.30161126853518283 1106 18-19 0.4143384110620474 1106 20-21 0.3793666831863831 1106 22-23 0.3854173208826168 1106 24-25 0.3286093574897535 1106 26-27 0.5428979441046309 1106 28-29 0.5884367622109252 1106 30-31 0.5671876506030173 1106 32-33 0.4653591188638373 1106 34-35 0.5377315511527669 1106 36-37 0.467382162071857 1106 38-39 0.42168268420372357 1106 40-41 0.4020695872602644 1106 42-43 0.28836353961845873 1106 44-45 0.24730410415659065 1106 46-47 0.26497106178975827 1106 48-49 0.46807884321466275 1106 50-51 0.567263871226757 1106 52-53 0.4448602639335846 1106 54-55 0.3251273854529444 1106 56-57 -0.023525707087266312 1106 58-59 0.4339680848619665 1106 60-61 0.370361722369573 1106 62-63 0.33605122462212833 1106 64-65 0.45579948924512337 1106 66-67 0.24662309366993895 1106 68-69 0.2973027086833717 1106 70-71 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-1.6325857738266336 2216 56-57 -1.4162268370057944 2216 58-59 -1.6654791492406673 2216 60-61 -1.4255117147761212 2216 62-63 -1.5601658729005514 2216 64-65 -1.3821206292373063 2216 66-67 -1.3314532353712352 2216 68-69 -1.2205632455865185 2216 70-71 -1.258886889930281 2216 72-73 -1.107832802492517 2216 74-75 -1.1564674511393598 2216 76-77 -1.4319434639961486 2216 78-79 -1.4548389949228913 2216 80-81 -1.2677820085156526 2216 82-83 -1.136261562661467 2216 84-85 -1.1808643163032144 2216 86-87 -1.3192939932649494 2216 88-89 -1.1904464098089562 2216 90-91 -1.2253020174816527 2216 92-93 -1.3675960901697017 2216 94-95 -1.2958944826420051 2216 96-97 -1.328824138220618 2216 98-99 -1.4078349010782851 2216 100-101 -1.4245237812643587 2216 102-103 -1.2639960760997688 2216 104-105 -1.299551218238335 2216 106-107 -1.4529037675471876 2216 108-109 -1.328853428202322 2216 110-111 -1.3172113566593673 2216 112-113 -1.2964568768818339 2216 114-115 -1.4724840339240721 2216 116-117 -1.4971733444846755 2216 118-119 -1.4496554821379455 2216 120-121 -1.5034563742657348 2216 122-123 -1.6390996304902359 2216 124-125 -1.972114703721843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70353.0 3 23095.0 4 11023.0 5 8801.0 6 9820.0 7 11810.0 8 13045.0 9 14593.0 10 16223.0 11 18158.0 12 20405.0 13 23567.0 14 27803.0 15 24198.0 16 16059.0 17 13720.0 18 12834.0 19 11951.0 20 11831.0 21 12334.0 22 13686.0 23 16858.0 24 21572.0 25 28330.0 26 36189.0 27 40973.0 28 46150.0 29 55315.0 30 64476.0 31 75186.0 32 92038.0 33 114607.0 34 147497.0 35 201598.0 36 321336.0 37 500554.0 38 194938.0 39 645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21803186616297 20.3075142385499 14.143818429926261 23.330635465360867 2 15.200356720729486 21.373033448968883 39.65578156402412 23.770828266277515 3 19.091932781212943 26.214311407676576 29.59914591877097 25.09460989233951 4 12.519749040364283 17.650121537484413 37.71365162158895 32.11647780056235 5 12.70104468778629 39.17726409825006 34.37920165422767 13.742489559735976 6 30.48774711753986 38.31545961270215 17.550439052198545 13.646354217559441 7 27.408665092395278 33.374524966311334 21.33531724526972 17.88149269602367 8 25.763726465395955 36.034566706395076 19.759060617194336 18.442646211014626 9 26.576154280420557 16.020026686887366 20.25050000576068 37.1533190269314 10-11 24.617237814143202 27.48431124141636 27.458090415552082 20.440360528888352 12-13 25.517724873707685 24.892397115342355 28.133028613171952 21.456849397778004 14-15 22.677607811502924 25.964090247803618 26.423090930309513 24.935211010383945 16-17 21.56511720937675 29.28449742646078 27.0130623783236 22.137322985838868 18-19 21.367262424179064 27.495002165550464 30.039598637090016 21.098136773180457 20-21 22.361638684757832 26.56229598154585 30.12603166665102 20.950033667045297 22-23 22.02111649273694 26.497938402548932 29.905729333568303 21.575215771145828 24-25 21.56766319433036 26.767783011481193 30.190465746503946 21.474088047684496 26-27 21.52898290685454 26.94917286482893 30.05345261568777 21.46839161262876 28-29 21.368778671523074 26.759377036155506 30.33914910194741 21.532695190374007 30-31 21.567471179665564 26.673695825729197 30.19550079771426 21.563332196890983 32-33 21.58648063148076 26.703244749145643 29.926552257217725 21.78372236215587 34-35 21.746364842370895 26.790440741927597 30.05240720251275 21.41078721318876 36-37 21.944459971556228 26.466618677223774 29.917719582636927 21.671201768583074 38-39 21.729424881942982 26.888944264969993 29.782221234176394 21.599409618910627 40-41 21.88130848179978 26.619227665814265 29.933507455076036 21.565956397309918 42-43 21.79315241569383 26.697356299425106 29.550096839395945 21.959394445485117 44-45 21.940064969228583 26.882373096441288 29.309097100109195 21.868464834220937 46-47 22.164807466895606 27.163802590149817 28.704506925542262 21.966883017412318 48-49 22.085249390780138 27.211358222131953 28.475284085696572 22.228108301391337 50-51 22.055923228237262 27.475155425269993 28.205664861728046 22.263256484764696 52-53 22.345450596546893 27.41681391346795 28.14670432429826 22.091031165686896 54-55 22.204191808142358 27.314896796384662 28.233238933234794 22.24767246223818 56-57 22.093327427344388 27.66759523833909 28.051441846492082 22.187635487824444 58-59 22.146655558175034 27.31014133457646 28.17557959550725 22.367623511741254 60-61 22.25537438379294 27.46899923236804 28.25645137271284 22.019175011126183 62-63 22.091799224346094 27.306618830835504 28.417466336629015 22.184115608189384 64-65 21.977329410534804 27.177910576408166 28.32101947247036 22.52374054058667 66-67 22.002491070251338 27.502367114117725 28.043869803816484 22.45127201181445 68-69 22.104657019985964 27.275529912810352 28.240233541185784 22.3795795260179 70-71 22.02211923598645 27.23243716533444 28.311836936026264 22.43360666265285 72-73 22.18735852252823 27.35778007152333 27.915860027283152 22.539001378665294 74-75 22.07650259233728 27.483874298790955 27.93095407100749 22.508669037864276 76-77 22.126425868898362 27.359230848990705 28.042611041013902 22.471732241097026 78-79 22.18816925111294 27.380843166262085 27.860794488411063 22.570193094213916 80-81 21.958860565723228 27.476071193200617 27.861765390484972 22.703302850591182 82-83 22.16333535446547 27.625960553360663 27.724506746328576 22.486197345845294 84-85 22.156934865638807 27.47864263553355 27.69369906010955 22.670723438718092 86-87 22.023847367969648 27.39902055452982 27.934336105029463 22.64279597247107 88-89 22.18808105268211 27.292437180971074 27.83808665318989 22.681395113156928 90-91 22.007291436871338 27.480178752851952 27.892412903214797 22.620116907061917 92-93 22.076522808497547 27.438448985043244 27.84343745646246 22.641590749996745 94-95 21.988815359125027 27.242208578276482 27.803787467928213 22.965188594670273 96-97 22.188182780281306 27.15912194455824 27.771170456097227 22.881524819063227 98-99 21.904758247178968 27.17790471216163 27.725810636202414 23.191526404456983 100-101 21.86070189055877 27.200824744555558 27.850660984031027 23.087812380854643 102-103 21.97396622504716 27.003619689781104 27.83167226424973 23.190741820922003 104-105 21.97328350623898 26.910727261943418 27.89094079078466 23.225048441032936 106-107 21.807499026042326 27.083682699311424 27.804347371551728 23.30447090309452 108-109 21.900872642646625 27.120727300346353 27.489203442097548 23.489196614909467 110-111 21.6646889122436 27.05917944739792 27.717790871777936 23.558340768580543 112-113 21.833390155450804 26.963019255657283 27.839651540320304 23.363939048571602 114-115 21.67984242849907 27.275235100622087 27.591781943026263 23.453140527852582 116-117 21.56945533120183 27.225481967476128 27.633641139952665 23.57142156136938 118-119 21.549891170354986 27.076883951883683 27.61851465135897 23.75471022640236 120-121 21.3613290617474 27.05120611995146 27.765977057009287 23.821487761291856 122-123 21.493807857973742 27.11126397189789 27.59211403338832 23.802814136740043 124-125 21.37583354456451 26.964081588549572 27.63836994590702 24.021714920978898 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 288.0 1 205.5 2 253.0 3 396.5 4 296.5 5 229.5 6 486.0 7 1109.5 8 1849.5 9 2183.0 10 1814.0 11 1319.0 12 1228.0 13 1327.5 14 1496.0 15 1749.5 16 2034.0 17 2289.0 18 2613.5 19 2956.5 20 3518.0 21 4273.0 22 5318.0 23 6588.5 24 7928.0 25 10141.0 26 13614.0 27 17609.5 28 20848.5 29 24811.5 30 30647.5 31 36699.0 32 42849.0 33 49348.5 34 56639.0 35 62720.0 36 69091.0 37 74941.0 38 77285.0 39 78723.5 40 78503.0 41 78969.5 42 79822.0 43 79890.5 44 80503.0 45 81850.0 46 82148.0 47 80606.5 48 81567.5 49 81468.5 50 78839.0 51 76259.0 52 73594.0 53 72009.5 54 70123.5 55 65422.5 56 59999.5 57 55359.5 58 51517.0 59 46473.0 60 39181.0 61 31844.5 62 25116.0 63 19061.5 64 13663.5 65 9995.0 66 7570.5 67 5677.0 68 4268.0 69 3136.5 70 2237.0 71 1699.0 72 1369.5 73 1236.5 74 977.0 75 701.5 76 499.0 77 293.0 78 196.0 79 115.5 80 68.0 81 51.5 82 40.0 83 25.5 84 21.5 85 12.0 86 5.5 87 3.0 88 2.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09455655493262205 2 4.2669925511111035E-5 3 0.0 4 0.09890888733475538 5 0.0 6 0.0 7 0.002261506052088885 8 0.006827188081777766 9 0.004181652700088882 10-11 5.973789571555545E-4 12-13 0.0 14-15 6.400488826666655E-5 16-17 3.626943668444438E-4 18-19 0.002389515828622218 20-21 0.0014081075418666642 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.2669925511111035E-5 52-53 0.0 54-55 0.0 56-57 0.007979276070577763 58-59 7.893936219555542E-4 60-61 0.0 62-63 0.0 64-65 2.560195530666662E-4 66-67 0.0 68-69 6.400488826666655E-5 70-71 0.0 72-73 0.0 74-75 0.0012587628025777756 76-77 0.0 78-79 0.0 80-81 1.9201466479999965E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014294425046222196 90-91 0.0 92-93 0.0020268214617777743 94-95 0.0 96-97 0.009003354282844428 98-99 0.004395002327644437 100-101 0.0024535207168888845 102-103 0.0 104-105 0.0 106-107 0.0017067970204444414 108-109 0.0 110-111 1.7067970204444414E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.814082867555538E-4 122-123 0.0049923812847999915 124-125 0.09129230563102206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2343571.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.10118057913044 #Duplication Level Percentage of deduplicated Percentage of total 1 73.92149384423169 37.03554111768931 2 12.94344053265602 12.96963302883671 3 5.101127490777564 7.667175287178399 4 2.5751242028311485 5.160670507989307 5 1.4540719956916204 3.6425361815601223 6 0.9098101021519008 2.73495361323777 7 0.6174702720313829 2.165519272090238 8 0.4319177375997646 1.7311670853452226 9 0.31098888802072544 1.4022819393146404 >10 1.5500309532338468 13.92944359066241 >50 0.119452151223797 4.184470808540488 >100 0.06250535207123413 5.7929296843952685 >500 0.00213873123267598 0.7109813181916268 >1k 2.566477479210699E-4 0.2986567292716748 >5k 1.710984986140466E-4 0.5740398356968341 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7245 0.3091436103279994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6148 0.2623347020423107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3804 0.16231639664426636 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.2669925511111035E-5 0.0 12-13 0.0 0.0 0.0 6.400488826666655E-5 0.0 14-15 0.0 0.0 0.0 1.280097765333331E-4 0.0 16-17 0.0 0.0 0.0 1.7067970204444414E-4 0.0 18-19 0.0 0.0 0.0 2.560195530666662E-4 0.0 20-21 0.0 0.0 0.0 3.413594040888883E-4 0.0 22-23 0.0 0.0 0.0 4.6936918062222136E-4 0.0 24-25 0.0 0.0 0.0 6.1871391991111E-4 0.0 26-27 0.0 0.0 0.0 8.960684357333317E-4 0.0 28-29 0.0 0.0 0.0 0.0017921368714666633 0.0 30-31 0.0 0.0 0.0 0.003968303072533326 0.0 32-33 0.0 0.0 0.0 0.008555320064977763 0.0 34-35 0.0 0.0 0.0 0.01504114874266664 0.0 36-37 0.0 0.0 0.0 0.027244747438844398 0.0 38-39 0.0 0.0 0.0 0.0477689816096888 0.0 40-41 0.0 0.0 0.0 0.07300824254951098 0.0 42-43 0.0 0.0 0.0 0.09594332751173316 0.0 44-45 0.0 0.0 0.0 0.12318807495057757 0.0 46-47 0.0 0.0 0.0 0.15738802024773307 0.0 48-49 0.0 0.0 0.0 0.19478820995822188 0.0 50-51 0.0 0.0 0.0 0.23765015013413293 0.0 52-53 0.0 0.0 0.0 0.2775251955242662 0.0 54-55 0.0 0.0 0.0 0.323182015821155 0.0 56-57 0.0 0.0 0.0 0.3785035742463104 2.1334962755555518E-5 58-59 0.0 0.0 0.0 0.44440727419822146 4.2669925511111035E-5 60-61 0.0 0.0 0.0 0.5178208810400879 4.2669925511111035E-5 62-63 0.0 0.0 0.0 0.59183186683911 4.2669925511111035E-5 64-65 0.0 0.0 0.0 0.6659495274519099 4.2669925511111035E-5 66-67 0.0 0.0 0.0 0.7551083368073764 4.2669925511111035E-5 68-69 0.0 0.0 0.0 0.8592229550544874 4.2669925511111035E-5 70-71 0.0 0.0 0.0 0.979573479958576 4.2669925511111035E-5 72-73 4.2669925511111035E-5 0.0 0.0 1.1016308018831091 4.2669925511111035E-5 74-75 4.2669925511111035E-5 0.0 0.0 1.2222373463402645 4.2669925511111035E-5 76-77 4.2669925511111035E-5 0.0 0.0 1.3565409368864865 4.2669925511111035E-5 78-79 4.2669925511111035E-5 0.0 0.0 1.5127768691454195 4.2669925511111035E-5 80-81 4.2669925511111035E-5 0.0 0.0 1.6899637348303083 4.2669925511111035E-5 82-83 4.2669925511111035E-5 0.0 0.0 1.879908908243019 4.2669925511111035E-5 84-85 4.2669925511111035E-5 0.0 0.0 2.069726071879196 4.2669925511111035E-5 86-87 4.2669925511111035E-5 0.0 0.0 2.269527998084974 4.2669925511111035E-5 88-89 4.2669925511111035E-5 0.0 0.0 2.5012470285730624 4.2669925511111035E-5 90-91 4.2669925511111035E-5 0.0 0.0 2.7687447916022174 4.2669925511111035E-5 92-93 4.2669925511111035E-5 0.0 0.0 3.0561907448078167 4.2669925511111035E-5 94-95 4.2669925511111035E-5 0.0 0.0 3.3409484927062163 4.2669925511111035E-5 96-97 4.2669925511111035E-5 0.0 0.0 3.634880274589505 4.2669925511111035E-5 98-99 4.2669925511111035E-5 0.0 0.0 3.9554594249544817 4.2669925511111035E-5 100-101 4.2669925511111035E-5 0.0 0.0 4.336736544359015 4.2669925511111035E-5 102-103 6.400488826666655E-5 0.0 0.0 4.73945530133288 4.2669925511111035E-5 104-105 8.533985102222207E-5 0.0 0.0 5.14076595076488 4.2669925511111035E-5 106-107 1.0667481377777759E-4 0.0 0.0 5.544828810392346 4.2669925511111035E-5 108-109 1.280097765333331E-4 0.0 0.0 5.970290637663634 4.2669925511111035E-5 110-111 1.280097765333331E-4 0.0 0.0 6.453164849710122 4.2669925511111035E-5 112-113 1.280097765333331E-4 0.0 0.0 6.974313984940077 4.2669925511111035E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5715 0.0 56.749004 1 GTATCAA 9625 0.0 50.853165 1 ATCAACG 12410 0.0 38.829563 3 TATCAAC 12585 0.0 38.100304 2 TCAACGC 12695 0.0 37.817093 4 CAACGCA 12650 0.0 37.53404 5 AACGCAG 13035 0.0 36.51673 6 GTACATG 11795 0.0 32.3993 1 ACGCAGA 15385 0.0 30.822918 7 TACATGG 12090 0.0 30.49651 2 CGCAGAG 15580 0.0 30.16981 8 ACATGGG 12235 0.0 29.599567 3 GCAGAGT 16695 0.0 27.405867 9 TGGTATC 2785 0.0 27.162159 2 GAGTACT 9870 0.0 26.012169 12-13 CGGTATA 280 9.276846E-11 25.527542 2 AGAGTAC 15635 0.0 24.562855 10-11 CATGGGG 4855 0.0 24.537285 4 CATGGGA 7730 0.0 24.195574 4 AGTACTT 10540 0.0 24.161066 12-13 >>END_MODULE