FastQCFastQC Report
Thu 26 May 2016
SRR1364578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943127
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.12330640251512123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30250.056.0548551
GTATCAA54750.050.7488861
ATCAACG62350.043.703043
TCAACGC62750.042.9503864
CAACGCA63600.042.2828145
AACGCAG65700.040.6554576
TATCAAC68600.039.374432
GTACATG102150.036.8105661
TACATGG100800.036.5353052
ACATGGG102850.034.7658423
ACGCAGA79200.033.3508457
CGCAGAG79100.032.345078
CATGGGA65650.029.362494
GCAGAGT87650.027.6971789
CATGGGG45300.026.3985734
ATGGGAG32450.022.9180625
CAGAGTA86800.022.83009310-11
GAGTACA76300.022.7694261
AGAGTAC83200.021.95827710-11
GAGTACT37150.021.86650712-13
Produced by FastQC (version 0.11.5)