##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364578_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943127 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.99358147974888 31.0 30.0 34.0 23.0 34.0 2 29.005711927218343 31.0 30.0 34.0 22.0 34.0 3 29.24414204527033 31.0 30.0 34.0 23.0 34.0 4 32.62026208271513 35.0 33.0 37.0 25.0 37.0 5 32.7846893177852 35.0 33.0 37.0 26.0 37.0 6 32.77777211679936 36.0 33.0 37.0 25.0 37.0 7 32.5687075523113 35.0 33.0 37.0 25.0 37.0 8 32.562389900402806 35.0 33.0 37.0 25.0 37.0 9 33.742603031093694 38.0 33.0 39.0 22.0 39.0 10-11 33.659860112077084 38.0 33.5 39.0 20.5 39.0 12-13 33.605560007143126 38.0 33.0 39.0 20.0 39.0 14-15 34.40288282752491 39.0 33.0 41.0 18.0 41.0 16-17 34.54614855333696 39.0 34.0 41.0 19.0 41.0 18-19 34.343113960127155 39.0 33.0 41.0 17.5 41.0 20-21 34.0614918633728 39.0 33.0 41.0 18.0 41.0 22-23 33.990406185493796 39.0 33.0 41.0 17.5 41.0 24-25 33.81923080683866 39.0 32.0 41.0 17.0 41.0 26-27 33.756526979451166 38.0 32.0 41.0 16.5 41.0 28-29 33.831204805450184 38.5 32.5 41.0 16.5 41.0 30-31 33.78541032058121 38.0 32.5 40.0 16.5 41.0 32-33 33.787122509233825 38.0 33.0 40.0 16.0 41.0 34-35 33.31617979679146 38.0 32.0 40.0 13.0 41.0 36-37 33.203114876176386 38.0 32.0 40.0 11.5 41.0 38-39 33.0087382348143 38.0 31.0 40.0 9.0 41.0 40-41 33.24897523424871 38.0 32.0 40.0 13.5 41.0 42-43 33.14778704634334 38.0 32.0 40.0 9.0 41.0 44-45 33.01147866300041 38.0 32.0 40.0 9.0 41.0 46-47 32.98952641798503 38.0 32.0 40.0 8.5 41.0 48-49 32.85591935061373 38.0 32.0 40.0 7.5 41.0 50-51 32.71690167446595 38.0 32.0 40.0 6.5 41.0 52-53 32.666164898125544 38.0 31.5 40.0 2.0 41.0 54-55 32.70066470179252 38.0 32.0 40.0 2.0 41.0 56-57 32.58133770978428 38.0 32.0 40.0 2.0 41.0 58-59 32.390190399289395 37.0 31.0 40.0 2.0 41.0 60-61 32.18571946146598 37.0 31.0 40.0 2.0 41.0 62-63 31.909750623608232 36.5 31.0 40.0 2.0 41.0 64-65 31.63373006499318 36.0 31.0 39.5 2.0 41.0 66-67 31.38417432313997 36.0 30.0 39.0 2.0 41.0 68-69 31.338897303161346 35.5 31.0 39.0 2.0 41.0 70-71 31.09530797523785 35.0 30.5 39.0 2.0 40.5 72-73 30.801686405469123 35.0 30.0 38.0 2.0 40.0 74-75 30.49012313657316 35.0 30.0 37.0 2.0 39.5 76-77 30.165724371078163 35.0 30.0 37.0 2.0 39.0 78-79 29.845896073699762 35.0 30.0 36.0 2.0 39.0 80-81 29.529012257047533 35.0 30.0 36.0 2.0 38.0 82-83 29.271589299103972 34.0 29.0 35.5 2.0 37.0 84-85 29.021209370257324 34.0 29.0 35.0 2.0 37.0 86-87 28.80853001373559 34.0 29.0 35.0 2.0 36.0 88-89 28.61163500893148 34.0 29.0 35.0 2.0 36.0 90-91 28.46802730855986 34.0 29.0 35.0 2.0 36.0 92-93 28.33602435661694 34.0 29.0 35.0 2.0 35.5 94-95 28.206379974134475 34.0 29.0 35.0 2.0 35.0 96-97 28.102180660347987 34.0 29.0 35.0 2.0 35.0 98-99 28.011274353143158 34.0 29.0 35.0 2.0 35.0 100-101 27.920160648274663 34.0 29.0 35.0 2.0 35.0 102-103 26.734114136646753 32.5 25.5 34.0 2.0 35.0 104-105 27.622098555575626 34.0 27.5 35.0 2.0 35.0 106-107 27.77002429589008 34.0 28.0 35.0 2.0 35.0 108-109 27.724653612450446 34.0 28.0 35.0 2.0 35.0 110-111 27.649881865673215 34.0 28.0 35.0 2.0 35.0 112-113 27.56554795440545 34.0 28.0 35.0 2.0 35.0 114-115 27.445955153729017 34.0 27.0 35.0 2.0 35.0 116-117 27.34019032209423 34.0 27.0 35.0 2.0 35.0 118-119 27.19990149897562 34.0 27.0 35.0 2.0 35.0 120-121 27.034665001309744 34.0 27.0 35.0 2.0 35.0 122-123 26.80863191134702 34.0 27.0 35.0 2.0 35.0 124-125 25.34600672009601 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.764160002666685 1101 2 0.8519623495968993 1101 3 0.8531646816994964 1101 4 0.8342515853583308 1101 5 0.9132685650649535 1101 6 0.9175695851401926 1101 7 0.9855696814411203 1101 8 0.8622826346111836 1101 9 1.168831393441586 1101 10-11 1.215023021139423 1101 12-13 1.0506567925372323 1101 14-15 1.385265675878614 1101 16-17 1.1624314774862938 1101 18-19 1.499520227872253 1101 20-21 1.7542447262705423 1101 22-23 1.6506544535304073 1101 24-25 1.9699790228856884 1101 26-27 1.967745721237904 1101 28-29 1.7543512050956096 1101 30-31 1.8325415550568422 1101 32-33 1.8388734771813589 1101 34-35 2.151672697334149 1101 36-37 1.9669791099797962 1101 38-39 1.9298269829510843 1101 40-41 1.640382922485884 1101 42-43 1.620471676848254 1101 44-45 1.519567736753963 1101 46-47 1.4173050893095223 1101 48-49 1.5222112384364053 1101 50-51 1.5365468998296308 1101 52-53 1.3986791469371695 1101 54-55 1.2778376504814517 1101 56-57 1.2643810188987317 1101 58-59 1.1958640870609756 1101 60-61 1.2977338913464536 1101 62-63 1.2800803090140178 1101 64-65 1.2985736598378246 1101 66-67 1.259238256113349 1101 68-69 1.1883073436462261 1101 70-71 1.1979316438867755 1101 72-73 0.9273547869731402 1101 74-75 1.0325920132890225 1101 76-77 1.1429194450192668 1101 78-79 1.1292219954060911 1101 80-81 1.1667811786614664 1101 82-83 1.0205535493601872 1101 84-85 1.0769371351273094 1101 86-87 0.9457003488511582 1101 88-89 0.994879435927448 1101 90-91 1.0218360203810661 1101 92-93 0.9022078351103993 1101 94-95 1.0215676259505848 1101 96-97 0.9504082949683301 1101 98-99 1.062741354502542 1101 100-101 1.0965056174486776 1101 102-103 1.0280455859763222 1101 104-105 1.178193957334539 1101 106-107 1.11680767005182 1101 108-109 1.143756386423071 1101 110-111 1.0839769934430485 1101 112-113 1.1168545318433303 1101 114-115 1.3746026609122026 1101 116-117 1.3727582122678292 1101 118-119 1.356196063669099 1101 120-121 1.4096734438865788 1101 122-123 1.5103188876317581 1101 124-125 1.630296108889567 1102 1 0.10643669327085448 1102 2 0.41725401844208676 1102 3 0.49030109501306285 1102 4 0.6093211161328398 1102 5 0.6068849318402343 1102 6 -0.060855189389229736 1102 7 -0.4869555737504001 1102 8 0.22842288601334815 1102 9 0.5897404229752254 1102 10-11 0.6303737765489643 1102 12-13 0.6546309985107612 1102 14-15 0.873266890097355 1102 16-17 0.7189265325398395 1102 18-19 0.9536179877097339 1102 20-21 1.358796599857783 1102 22-23 1.1231871960657713 1102 24-25 1.2361926756384136 1102 26-27 0.9090479911467497 1102 28-29 1.3243700528974571 1102 30-31 1.2459209816633532 1102 32-33 1.0360189209930368 1102 34-35 1.3708913384345394 1102 36-37 1.46819700894482 1102 38-39 1.5006330170903297 1102 40-41 1.1997476607004245 1102 42-43 1.143986124376191 1102 44-45 1.1471628628230661 1102 46-47 0.8864649737963077 1102 48-49 0.5637785603175729 1102 50-51 0.966303939328796 1102 52-53 0.706155932364112 1102 54-55 0.5659309717199719 1102 56-57 0.5277507500294831 1102 58-59 0.7358778485451651 1102 60-61 0.6495667169786472 1102 62-63 0.46064801211520745 1102 64-65 0.4591435558424273 1102 66-67 0.51687166662132 1102 68-69 0.46329552887143777 1102 70-71 0.45162785366641245 1102 72-73 0.6252697774477163 1102 74-75 0.28547197568479277 1102 76-77 0.44884939596828133 1102 78-79 0.42201191170520147 1102 80-81 0.16919654502608594 1102 82-83 0.28535184031684935 1102 84-85 -0.038067578092196186 1102 86-87 0.46799093291981464 1102 88-89 0.6663434172349803 1102 90-91 0.06261615592482883 1102 92-93 0.46840801064419324 1102 94-95 0.5364263232745792 1102 96-97 0.5882643816205011 1102 98-99 0.7361421466888984 1102 100-101 0.7722026130187061 1102 102-103 0.7206359328357763 1102 104-105 0.8676066247020593 1102 106-107 0.7796430586398913 1102 108-109 0.30288236720166495 1102 110-111 0.5074866693755808 1102 112-113 0.2333332365683809 1102 114-115 0.2730976286557123 1102 116-117 0.1653971126485949 1102 118-119 0.4859607427970758 1102 120-121 0.8101471008728289 1102 122-123 0.6598977435757654 1102 124-125 0.9660716276609733 1103 1 0.6944156629185727 1103 2 0.7085425892900012 1103 3 0.5794077236792212 1103 4 0.6812417595873299 1103 5 0.7803227961664518 1103 6 0.826305076215128 1103 7 0.822454448346086 1103 8 0.9121300716966374 1103 9 1.244908282929842 1103 10-11 1.2499514924134019 1103 12-13 1.1949003756862524 1103 14-15 1.455820562683229 1103 16-17 1.2222509002503017 1103 18-19 1.321183703120525 1103 20-21 1.7608721191403873 1103 22-23 1.8007951406512746 1103 24-25 1.8051998510967806 1103 26-27 1.7307430774255366 1103 28-29 1.6579022605085 1103 30-31 1.575261415945917 1103 32-33 1.600523642851094 1103 34-35 1.99186343658058 1103 36-37 1.9580180753761027 1103 38-39 1.963498478704885 1103 40-41 1.596968689733373 1103 42-43 1.471682706081296 1103 44-45 1.171314733590222 1103 46-47 1.2762936772028297 1103 48-49 1.2486953566140713 1103 50-51 1.2601198795990172 1103 52-53 1.1999497482409467 1103 54-55 1.1060609931263414 1103 56-57 1.032038175838558 1103 58-59 0.969165685634529 1103 60-61 0.8734339899010592 1103 62-63 0.9705038331268341 1103 64-65 1.1243759215972169 1103 66-67 1.0969512510074821 1103 68-69 1.1094197893090332 1103 70-71 1.042884950534976 1103 72-73 1.076257205362662 1103 74-75 1.0641632797579348 1103 76-77 1.0455241782954587 1103 78-79 1.1003710580994195 1103 80-81 1.036259387183506 1103 82-83 1.0586359248858201 1103 84-85 1.0259928164273475 1103 86-87 0.9473240981212889 1103 88-89 0.9303866349032326 1103 90-91 0.9577273119725902 1103 92-93 1.0014393240187225 1103 94-95 0.9485172535165205 1103 96-97 0.9692939448337263 1103 98-99 0.8735616508132473 1103 100-101 0.7835148508782623 1103 102-103 0.7328542686297723 1103 104-105 0.764022092393212 1103 106-107 0.742200060033575 1103 108-109 0.8374144350129313 1103 110-111 0.8638234939821672 1103 112-113 1.0026583360076344 1103 114-115 0.9974154450592749 1103 116-117 0.9539291413869613 1103 118-119 0.9976866712921506 1103 120-121 0.9647136441723241 1103 122-123 0.9535592317304626 1103 124-125 0.9889056127589768 1104 1 0.7559201535651248 1104 2 0.8747020911106453 1104 3 0.8576002446735203 1104 4 0.8992776130594606 1104 5 0.9706705829658517 1104 6 0.9155104541172392 1104 7 0.7961574149102404 1104 8 0.9407231765497741 1104 9 1.163437515164496 1104 10-11 1.1160262003237236 1104 12-13 1.1416715712554577 1104 14-15 1.1812741917035225 1104 16-17 1.1627956848015941 1104 18-19 1.484420764956326 1104 20-21 1.7572702476201982 1104 22-23 1.4969435289259536 1104 24-25 1.8195472703728086 1104 26-27 1.8497110822365812 1104 28-29 1.7466709076307438 1104 30-31 1.617381956965012 1104 32-33 1.6030428873768798 1104 34-35 1.9709271874035252 1104 36-37 1.9854878953679957 1104 38-39 1.9932689997689863 1104 40-41 1.7254821526511392 1104 42-43 1.7559995482672832 1104 44-45 1.7655420999789726 1104 46-47 1.6006102629771561 1104 48-49 1.4172191875846565 1104 50-51 1.4047961207085962 1104 52-53 1.5605680517122096 1104 54-55 1.4630188421364352 1104 56-57 1.4045306538364102 1104 58-59 1.2765525730747953 1104 60-61 1.328714075713492 1104 62-63 1.3959407479058044 1104 64-65 1.3673590860351972 1104 66-67 1.3936313492131518 1104 68-69 1.391612569564007 1104 70-71 1.439656644682401 1104 72-73 1.3118807617116168 1104 74-75 1.396705634571365 1104 76-77 1.3073942573580908 1104 78-79 1.3835047266830784 1104 80-81 1.420346657840497 1104 82-83 1.3297400287306402 1104 84-85 1.3404035432665538 1104 86-87 1.2905018386455147 1104 88-89 1.3479590124352114 1104 90-91 1.4149287639273425 1104 92-93 1.4609904445495303 1104 94-95 1.4351092980599738 1104 96-97 1.2382913182761754 1104 98-99 1.357299928586368 1104 100-101 1.514543890210625 1104 102-103 1.404813006771029 1104 104-105 1.4833072997910683 1104 106-107 1.4379714282058131 1104 108-109 1.5225988507872792 1104 110-111 1.4706986621039277 1104 112-113 1.5526027564068912 1104 114-115 1.586750393944321 1104 116-117 1.6038494757097759 1104 118-119 1.5246410563707045 1104 120-121 1.5725631765890462 1104 122-123 1.573979048837728 1104 124-125 1.9378711460465503 1105 1 0.2376200710448586 1105 2 0.5526565918560671 1105 3 0.5835390143109862 1105 4 0.6495782093281903 1105 5 0.5684481563590751 1105 6 0.5945033677793887 1105 7 0.5925798257701231 1105 8 0.3829078942052746 1105 9 0.5329661927569092 1105 10-11 0.7418118325072314 1105 12-13 0.632383918800258 1105 14-15 0.8704130568443134 1105 16-17 0.8837388777389918 1105 18-19 0.9485499392496735 1105 20-21 1.2363096819952162 1105 22-23 1.4451082368685348 1105 24-25 1.518925569702823 1105 26-27 1.2142663634987656 1105 28-29 1.0917711013547944 1105 30-31 1.0173465728881084 1105 32-33 1.042034793051549 1105 34-35 1.680416832190133 1105 36-37 1.6363901835228205 1105 38-39 1.707474063442902 1105 40-41 1.2865526655565134 1105 42-43 0.9267496094084606 1105 44-45 1.0111322127829965 1105 46-47 0.9979470857123403 1105 48-49 0.845386724395361 1105 50-51 0.8093398217274341 1105 52-53 0.9026798180151872 1105 54-55 0.6749070442467584 1105 56-57 0.7231041123181328 1105 58-59 0.7238269307788912 1105 60-61 0.6870094411310177 1105 62-63 0.7545642318310755 1105 64-65 0.8544711298831196 1105 66-67 0.6124225237020582 1105 68-69 0.42951615068342974 1105 70-71 0.6020556804517874 1105 72-73 0.7533330626520893 1105 74-75 0.7754017144987344 1105 76-77 0.7730372709248172 1105 78-79 0.805056126264418 1105 80-81 0.7224402176597913 1105 82-83 0.6551255316873679 1105 84-85 0.6406595000893844 1105 86-87 0.6733117384761762 1105 88-89 0.8393033843928102 1105 90-91 0.675766313272856 1105 92-93 0.5774319862687172 1105 94-95 0.6833816098281815 1105 96-97 0.8673862384140705 1105 98-99 0.7042147320439085 1105 100-101 0.6704005878345853 1105 102-103 0.6678256839556056 1105 104-105 0.7417169115324036 1105 106-107 0.7183383867475648 1105 108-109 0.620038725146518 1105 110-111 0.6918855487905766 1105 112-113 0.6628602716619909 1105 114-115 0.734803259404238 1105 116-117 0.739874019508342 1105 118-119 0.7056166744539993 1105 120-121 0.6417318470372955 1105 122-123 0.8432382871940227 1105 124-125 0.9607036884348616 1106 1 0.5623519236551822 1106 2 0.8334858604171025 1106 3 0.6000964579621986 1106 4 0.7502411789277588 1106 5 0.6684281529155314 1106 6 0.5693907942749306 1106 7 0.4029934148151142 1106 8 0.6217622364799951 1106 9 0.7708410802887471 1106 10-11 0.9420215467833799 1106 12-13 0.9307573427097395 1106 14-15 1.1972491335990156 1106 16-17 1.046001216873094 1106 18-19 0.9968432147801991 1106 20-21 1.4207988433242207 1106 22-23 1.4260164774796493 1106 24-25 1.4613738249846833 1106 26-27 1.737719786551466 1106 28-29 1.61022003504484 1106 30-31 1.4399345924289761 1106 32-33 1.2495764307050266 1106 34-35 1.5604665165769944 1106 36-37 1.4979916950293628 1106 38-39 1.7915238161855953 1106 40-41 1.4534248093743685 1106 42-43 1.4337385831685978 1106 44-45 1.2823958791204007 1106 46-47 1.0374234543183292 1106 48-49 1.1237249848495594 1106 50-51 1.0111129414768953 1106 52-53 0.9653176343086756 1106 54-55 0.9254005464049371 1106 56-57 0.9456155592857698 1106 58-59 0.87925456495676 1106 60-61 0.9880592766264016 1106 62-63 0.8523273458791465 1106 64-65 0.852696219304601 1106 66-67 0.7814197261461544 1106 68-69 0.7377624854240317 1106 70-71 0.8665552215664718 1106 72-73 0.7486608715703902 1106 74-75 0.7464541917595966 1106 76-77 0.7688153036323762 1106 78-79 0.7947245056893593 1106 80-81 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2216 42-43 -5.766861444938314 2216 44-45 -5.617827199321717 2216 46-47 -3.9862619719638133 2216 48-49 -2.668875365113834 2216 50-51 -2.4548162080991425 2216 52-53 -2.6751178471979102 2216 54-55 -2.3383781011436433 2216 56-57 -2.1390873635506544 2216 58-59 -2.055586257428942 2216 60-61 -2.303446731024664 2216 62-63 -2.4053065486367338 2216 64-65 -2.6188249341773293 2216 66-67 -2.391925176386259 2216 68-69 -2.1087681581798954 2216 70-71 -2.1738783518198304 2216 72-73 -2.2093704773313156 2216 74-75 -2.077395363153684 2216 76-77 -2.112830709567376 2216 78-79 -2.4081540603249394 2216 80-81 -2.278961761881945 2216 82-83 -1.9912251787033775 2216 84-85 -2.0943941511720396 2216 86-87 -2.0986052210745534 2216 88-89 -2.174765756163257 2216 90-91 -2.1979268123468216 2216 92-93 -2.235657989388347 2216 94-95 -2.2051673901435365 2216 96-97 -2.126076551467463 2216 98-99 -2.201686528283716 2216 100-101 -2.2709101664379787 2216 102-103 -2.1671474416162724 2216 104-105 -2.3093578465021416 2216 106-107 -2.2908696850216757 2216 108-109 -2.3830101172792695 2216 110-111 -2.379946782104483 2216 112-113 -2.42978460387274 2216 114-115 -2.5273155780302687 2216 116-117 -2.6244428326482456 2216 118-119 -2.7108674500546925 2216 120-121 -2.709308573552587 2216 122-123 -2.773385786302452 2216 124-125 -2.867291606299517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95600.0 3 16522.0 4 10431.0 5 8281.0 6 7574.0 7 8062.0 8 8723.0 9 9125.0 10 9833.0 11 10827.0 12 11688.0 13 13049.0 14 15176.0 15 13553.0 16 10398.0 17 9310.0 18 8590.0 19 8152.0 20 8195.0 21 8633.0 22 10400.0 23 13642.0 24 17874.0 25 23186.0 26 28548.0 27 33043.0 28 37955.0 29 45439.0 30 52470.0 31 60526.0 32 74944.0 33 96381.0 34 127054.0 35 177288.0 36 279886.0 37 412056.0 38 160002.0 39 711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77162465554033 18.912264050917752 13.039747840596883 23.276363452945034 2 14.81706024155912 20.094243877132996 41.58490492237952 23.503790958928363 3 19.434293984316938 25.629442899287074 28.583501287707385 26.3527618286886 4 11.945597305598788 16.42698477399804 38.20813467065227 33.4192832497509 5 13.265965260474383 38.713823288257146 34.22276140471337 13.7974500465551 6 31.397945971093733 37.028461619220344 17.970025340845698 13.603567068840231 7 28.545253634768606 32.720611887671 20.848947308014377 17.885187169546022 8 25.826104006583208 36.22835769355271 19.68198681815445 18.263551481709637 9 26.60540637906411 16.654375152786177 19.711076515317217 37.0291419528325 10-11 24.727668340772375 27.39792612629025 27.475970433224383 20.398435099712987 12-13 25.504766860151086 24.63430569612061 27.836101017446534 22.024826426281766 14-15 22.436360538553775 25.87429541496298 25.765967999015814 25.923376047467432 16-17 22.233733727807607 29.427784078360837 26.10734820728535 22.23113398654621 18-19 22.089884697028296 28.021115675331824 28.24177829107033 21.647221336569547 20-21 23.02277315498072 27.211469054423194 28.281095798051176 21.48466199254491 22-23 22.630368857890193 27.27492386492151 27.930734126141704 22.163973151046594 24-25 22.254364936904803 27.292999244684808 28.217288868815775 22.235346949594614 26-27 22.25650974676504 27.6000188395506 27.849179617356373 22.294291796327986 28-29 22.119068970353055 27.534927764275707 28.207532483238918 22.138470782132323 30-31 22.284116685129504 27.346433584608043 28.07442260211493 22.295027128147527 32-33 22.166885406212202 27.387889549396537 27.96235106410355 22.482873980287707 34-35 22.40420747502603 27.32244149665221 28.102529024567623 22.170822003754136 36-37 22.397308941346562 27.249387957095617 27.934042195639098 22.419260905918716 38-39 22.290102499733678 27.37178269871192 28.139668688665232 22.198446112889172 40-41 22.58165311787041 27.240750578603613 27.893532817737803 22.284063485788177 42-43 22.23739983185107 27.344814159920595 27.840331089203087 22.57745491902525 44-45 22.43660518276598 27.460784389420347 27.65342021891254 22.44919020890114 46-47 22.43254523960612 27.67120954091083 27.321217286493404 22.57502793298965 48-49 22.60797631218335 27.567252581846407 27.221015936357524 22.603755169612715 50-51 22.474753453097676 27.66899051897081 27.325044595113425 22.531211432818093 52-53 22.686345256897773 27.700839934806115 27.108804519725165 22.504010288570946 54-55 22.510138316569755 27.495485145181824 27.2961546588386 22.698221879409815 56-57 22.45225294264023 27.8144316685499 27.16760536596149 22.565710022848386 58-59 22.583330395623335 27.492797035288802 27.2284031354672 22.695469433620662 60-61 22.649762897071493 27.440997036599384 27.42885110841888 22.48038895791024 62-63 22.525936362798664 27.530741919226497 27.445209707069544 22.498112010905295 64-65 22.362953032432543 27.48436434744362 27.336301454300347 22.81638116582349 66-67 22.38525372469128 27.82916096373774 27.070443041345943 22.715142270225037 68-69 22.318084260045932 27.591537293238233 27.363448500757592 22.726929945958243 70-71 22.218542466949106 27.535402848284978 27.409081738944263 22.83697294582165 72-73 22.38985271489597 27.57204632644222 27.271973933334003 22.76612702532781 74-75 22.338200832524286 27.692432788086624 27.152220918195496 22.817145461193597 76-77 22.46485134437898 27.459093488282925 27.250060795240344 22.825994372097753 78-79 22.387470170196494 27.61767622678525 27.184143745043375 22.81070985797488 80-81 22.261601759907652 27.726599818378933 27.28492455057277 22.726873871140647 82-83 22.41171988643864 27.69963622384032 27.1979354538556 22.690708435865442 84-85 22.47673466531009 27.601747080865017 27.137958558550213 22.783559695274676 86-87 22.369013595936607 27.330652951842172 27.4749653500991 22.825368102122123 88-89 22.445052399678808 27.32414194239124 27.37430253865634 22.85650311927361 90-91 22.420231638329376 27.63190101307988 27.329606275582126 22.618261073008618 92-93 22.408117208538712 27.42401375169998 27.29287852879455 22.87499051096675 94-95 22.26406459769547 27.43880906492715 27.300283948176883 22.9968423892005 96-97 22.384344751085454 27.324442088087153 27.377099810318956 22.914113350508433 98-99 22.213925080238823 27.231151370833956 27.31158961775364 23.243333931173577 100-101 22.0594241227753 27.32695414120364 27.534732953786584 23.078888782234475 102-103 22.23871625477902 27.206533592503217 27.45108785992887 23.10366229278889 104-105 22.3357296845802 27.12461523972347 27.516438997001174 23.023216078695153 106-107 22.05652697162381 27.26813064827774 27.539677699664793 23.135664680433663 108-109 22.13810522935454 27.363600011733663 27.206662251103502 23.29163250780829 110-111 22.00078688470595 27.291595850045198 27.512170594957404 23.195446670291446 112-113 21.990482351385165 27.38542051034235 27.473603114979106 23.150494023293383 114-115 22.007491018343114 27.366250378899576 27.39903259025272 23.227226012504588 116-117 21.809228115300748 27.311107302816545 27.5307018017865 23.348962780096205 118-119 21.885291079790463 27.29960522395088 27.371293796030834 23.44380990022783 120-121 21.80619409615802 27.30362876902638 27.612203952963384 23.277973181852218 122-123 21.91166609284931 27.333493899266493 27.440177610624524 23.314662397259674 124-125 21.80639767989775 27.24611913585045 27.37339222813103 23.57409095612077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 547.0 1 501.5 2 573.0 3 669.0 4 565.5 5 427.5 6 376.0 7 466.5 8 646.5 9 756.0 10 732.0 11 730.5 12 804.0 13 861.0 14 977.0 15 1132.5 16 1266.0 17 1411.0 18 1590.5 19 1834.5 20 2179.0 21 2672.0 22 3341.5 23 4248.5 24 5410.0 25 7133.5 26 9728.0 27 12971.5 28 16048.5 29 19451.0 30 23675.5 31 27235.0 32 30928.0 33 35468.5 34 40033.0 35 44153.5 36 49014.5 37 53961.0 38 56928.0 39 58968.0 40 60606.5 41 63064.5 42 65635.5 43 67999.5 44 70185.0 45 71515.0 46 71798.0 47 71026.5 48 72815.5 49 74534.5 50 73260.0 51 71285.5 52 69057.0 53 67062.0 54 64196.0 55 58893.5 56 52754.5 57 47336.0 58 43815.5 59 40555.0 60 35149.5 61 28191.0 62 21292.0 63 15812.0 64 11572.0 65 8512.0 66 6294.5 67 4845.0 68 3733.5 69 2602.5 70 1876.0 71 1431.0 72 1058.5 73 878.0 74 684.0 75 495.5 76 357.5 77 220.0 78 140.5 79 104.5 80 68.5 81 48.0 82 38.5 83 27.5 84 26.0 85 24.0 86 19.5 87 15.0 88 9.0 89 8.0 90 10.0 91 7.0 92 3.5 93 5.5 94 7.0 95 4.5 96 5.5 97 4.0 98 0.5 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011785127786294976 2 0.0042714655295304944 3 0.007153418175960706 4 0.006947564415501406 5 0.01379220195077316 6 9.778053621816794E-4 7 0.008028296657912735 8 0.0 9 0.0026760988859709117 10-11 0.0 12-13 0.00393695316878413 14-15 0.02086842496656163 16-17 0.03221611351188059 18-19 0.005944027333262314 20-21 0.008208418698314623 22-23 0.0017754886839614703 24-25 0.01181085950635239 26-27 0.02086842496656163 28-29 4.889026810908397E-4 30-31 0.0017754886839614703 32-33 0.009109028900324065 34-35 0.010498541783424346 36-37 0.01278866486853407 38-39 0.0 40-41 0.026709525419594295 42-43 0.01062720038371141 44-45 0.017600496519270228 46-47 0.004322928969645319 48-49 0.027018306060283243 50-51 0.004580246170219446 52-53 0.0 54-55 0.019221594882887223 56-57 0.00522353917165476 58-59 1.801220404018883E-4 60-61 0.004580246170219446 62-63 0.030054649027057935 64-65 0.019118668002657574 66-67 0.002393049965339373 68-69 0.02380184105310667 70-71 0.0032421967272339893 72-73 0.013509153030141621 74-75 2.5731720057412612E-5 76-77 0.006664515494869867 78-79 0.0010292688022965047 80-81 0.0036281725280951785 82-83 0.001878415564191121 84-85 0.0 86-87 0.006175612813779028 88-89 0.018887082522140858 90-91 0.02503696361586247 92-93 0.004863295090850984 94-95 0.026709525419594295 96-97 0.020173668525011488 98-99 0.0011064639624687425 100-101 0.010061102542448332 102-103 0.0 104-105 0.0017497569639040576 106-107 7.719516017223784E-5 108-109 0.0 110-111 0.0011064639624687425 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.2865860028706309E-4 122-123 0.0 124-125 0.023364401812130654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1943127.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.634187815457466 #Duplication Level Percentage of deduplicated Percentage of total 1 78.70643798464953 47.72300943046894 2 12.07392256947767 14.641849774939997 3 3.9809041686483018 7.241366731114761 4 1.7875859444643976 4.335552875717048 5 0.9543298830983477 2.893250868484439 6 0.5652011241278793 2.0562306668326515 7 0.36025733188800196 1.5290737506514893 8 0.270820753843459 1.3136797162294451 9 0.19097431199712758 1.0421615071405443 >10 1.0135026683498334 11.092308308612823 >50 0.06711375588966416 2.811511689113825 >100 0.02851760157908027 2.9788270756571142 >500 1.7276079466545995E-4 0.0685078764796414 >1k 2.591411919981899E-4 0.2726697285573495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2396 0.12330640251512123 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.1463440114825225E-5 0.0 5 0.0 0.0 0.0 5.1463440114825225E-5 0.0 6 0.0 0.0 0.0 5.1463440114825225E-5 0.0 7 0.0 0.0 0.0 5.1463440114825225E-5 0.0 8 0.0 0.0 0.0 5.1463440114825225E-5 0.0 9 0.0 0.0 0.0 5.1463440114825225E-5 0.0 10-11 0.0 2.5731720057412612E-5 0.0 7.719516017223784E-5 0.0 12-13 0.0 5.1463440114825225E-5 0.0 1.0292688022965045E-4 0.0 14-15 0.0 5.1463440114825225E-5 0.0 1.0292688022965045E-4 0.0 16-17 0.0 5.1463440114825225E-5 0.0 1.0292688022965045E-4 0.0 18-19 0.0 5.1463440114825225E-5 0.0 2.3158548051671352E-4 0.0 20-21 0.0 5.1463440114825225E-5 0.0 2.573172005741261E-4 0.0 22-23 0.0 5.1463440114825225E-5 0.0 3.859758008611892E-4 0.0 24-25 0.0 5.1463440114825225E-5 0.0 4.889026810908396E-4 0.0 26-27 0.0 5.1463440114825225E-5 0.0 7.462198816649658E-4 0.0 28-29 5.1463440114825225E-5 5.1463440114825225E-5 0.0 0.001466708043272519 0.0 30-31 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.003190733287119164 2.5731720057412612E-5 32-33 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.007590857416936721 5.1463440114825225E-5 34-35 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.013020250349050782 5.1463440114825225E-5 36-37 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.02385330449322149 5.1463440114825225E-5 38-39 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.04317782625633837 5.1463440114825225E-5 40-41 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.06677381354898573 5.1463440114825225E-5 42-43 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.08756504335537513 5.1463440114825225E-5 44-45 1.0292688022965045E-4 5.1463440114825225E-5 0.0 0.11175286020934298 5.1463440114825225E-5 46-47 1.2865860028706306E-4 5.1463440114825225E-5 0.0 0.1430169000790993 7.719516017223784E-5 48-49 1.5439032034447568E-4 5.1463440114825225E-5 0.0 0.18079106512338103 1.0292688022965045E-4 50-51 1.5439032034447568E-4 5.1463440114825225E-5 0.0 0.22257937849661913 1.0292688022965045E-4 52-53 1.5439032034447568E-4 5.1463440114825225E-5 0.0 0.2650881800314647 1.0292688022965045E-4 54-55 1.5439032034447568E-4 5.1463440114825225E-5 0.0 0.30934673853021444 1.0292688022965045E-4 56-57 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.3631517651702642 1.0292688022965045E-4 58-59 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.4260143572705232 1.0292688022965045E-4 60-61 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.49476951326392976 1.0292688022965045E-4 62-63 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.5639363767782548 2.058537604593009E-4 64-65 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.639098731065957 2.058537604593009E-4 66-67 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.7248110905771985 2.058537604593009E-4 68-69 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.8235437004374907 2.058537604593009E-4 70-71 2.058537604593009E-4 5.1463440114825225E-5 0.0 0.9348591214058577 2.058537604593009E-4 72-73 2.058537604593009E-4 5.1463440114825225E-5 0.0 1.0542028390321374 2.3158548051671352E-4 74-75 2.058537604593009E-4 5.1463440114825225E-5 0.0 1.1695324083294607 2.573172005741261E-4 76-77 2.058537604593009E-4 5.1463440114825225E-5 0.0 1.2978050328156625 2.573172005741261E-4 78-79 2.058537604593009E-4 5.1463440114825225E-5 0.0 1.4495964494343396 2.573172005741261E-4 80-81 2.573172005741261E-4 5.1463440114825225E-5 0.0 1.622848120580899 2.573172005741261E-4 82-83 2.573172005741261E-4 5.1463440114825225E-5 0.0 1.8087597979957049 3.0878064068895136E-4 84-85 2.573172005741261E-4 5.1463440114825225E-5 0.0 1.9908374491219565 3.0878064068895136E-4 86-87 2.573172005741261E-4 5.1463440114825225E-5 0.0 2.1832335199912305 3.0878064068895136E-4 88-89 2.573172005741261E-4 5.1463440114825225E-5 0.0 2.401953140479238 3.0878064068895136E-4 90-91 2.573172005741261E-4 5.1463440114825225E-5 0.0 2.6564398518470487 3.0878064068895136E-4 92-93 2.573172005741261E-4 5.1463440114825225E-5 0.0 2.9320780371020527 3.0878064068895136E-4 94-95 2.573172005741261E-4 5.1463440114825225E-5 0.0 3.208668295999181 3.0878064068895136E-4 96-97 2.573172005741261E-4 5.1463440114825225E-5 0.0 3.4864936774590647 3.0878064068895136E-4 98-99 2.573172005741261E-4 5.1463440114825225E-5 0.0 3.7944251713861217 3.0878064068895136E-4 100-101 2.830489206315387E-4 5.1463440114825225E-5 0.0 4.153639983387602 3.0878064068895136E-4 102-103 3.0878064068895136E-4 5.1463440114825225E-5 0.0 4.543038103016427 3.0878064068895136E-4 104-105 4.374392409760144E-4 5.1463440114825225E-5 0.0 4.92767585443463 3.0878064068895136E-4 106-107 4.6317096103342704E-4 5.1463440114825225E-5 0.0 5.316507876222192 3.0878064068895136E-4 108-109 4.6317096103342704E-4 5.1463440114825225E-5 0.0 5.720933320364546 3.0878064068895136E-4 110-111 4.6317096103342704E-4 5.1463440114825225E-5 0.0 6.175638545499085 3.0878064068895136E-4 112-113 4.6317096103342704E-4 5.1463440114825225E-5 0.0 6.679182575302592 3.602440808037766E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3025 0.0 56.054855 1 GTATCAA 5475 0.0 50.748886 1 ATCAACG 6235 0.0 43.70304 3 TCAACGC 6275 0.0 42.950386 4 CAACGCA 6360 0.0 42.282814 5 AACGCAG 6570 0.0 40.655457 6 TATCAAC 6860 0.0 39.37443 2 GTACATG 10215 0.0 36.810566 1 TACATGG 10080 0.0 36.535305 2 ACATGGG 10285 0.0 34.765842 3 ACGCAGA 7920 0.0 33.350845 7 CGCAGAG 7910 0.0 32.34507 8 CATGGGA 6565 0.0 29.36249 4 GCAGAGT 8765 0.0 27.697178 9 CATGGGG 4530 0.0 26.398573 4 ATGGGAG 3245 0.0 22.918062 5 CAGAGTA 8680 0.0 22.830093 10-11 GAGTACA 7630 0.0 22.769426 1 AGAGTAC 8320 0.0 21.958277 10-11 GAGTACT 3715 0.0 21.866507 12-13 >>END_MODULE