FastQCFastQC Report
Thu 26 May 2016
SRR1364578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943127
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51590.26549988755238335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.23101938267545047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.14672226776736672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.064.100451
GTATCAA72000.049.083671
ATCAACG87300.040.0408253
TCAACGC88200.039.497214
CAACGCA90050.038.8521465
TATCAAC91500.038.007642
AACGCAG92700.037.8698546
GTACATG94550.031.3893781
ACGCAGA111850.031.1733177
CGCAGAG112250.031.0092248
TACATGG97400.029.5914022
ACATGGG95200.029.0867483
TGGTATC20750.028.985612
GCAGAGT123500.027.6538229
GAGTACT68000.026.8613912-13
GTGGTAT24400.025.889931
GTATACG1651.0965936E-525.283021
CAGAGTA118950.024.53334810-11
GTACTTT74750.024.51537514-15
CATGGGA65450.023.747074
Produced by FastQC (version 0.11.5)