##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364578_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943127 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.25232524688299 31.0 30.0 34.0 26.0 34.0 2 30.127175938577356 31.0 30.0 34.0 26.0 34.0 3 30.38951648554109 31.0 30.0 34.0 26.0 34.0 4 33.95802333043594 35.0 35.0 37.0 30.0 37.0 5 33.43026986913362 35.0 35.0 37.0 28.0 37.0 6 33.46891685412225 36.0 35.0 37.0 28.0 37.0 7 33.26885581848227 36.0 35.0 37.0 27.0 37.0 8 33.20253231003429 36.0 35.0 37.0 26.0 37.0 9 34.45366103193461 38.0 34.0 39.0 26.0 39.0 10-11 34.403217854520065 38.0 34.0 39.0 26.0 39.0 12-13 34.29273897177076 38.0 34.0 39.0 25.5 39.0 14-15 35.29239210818439 39.0 34.0 41.0 25.5 41.0 16-17 35.302564886391885 39.0 34.0 41.0 26.0 41.0 18-19 35.31840250277002 39.0 34.0 41.0 25.5 41.0 20-21 35.339761117003675 39.0 34.0 41.0 25.5 41.0 22-23 35.3004682658416 39.0 34.0 41.0 25.0 41.0 24-25 35.265607446142226 39.0 34.0 41.0 25.0 41.0 26-27 35.158068927043885 39.0 34.0 41.0 25.0 41.0 28-29 35.013319252936114 39.0 34.0 41.0 24.5 41.0 30-31 34.863283254259755 39.0 34.0 41.0 24.0 41.0 32-33 34.675722945540876 38.5 34.0 41.0 24.0 41.0 34-35 34.50740250122612 38.0 33.0 41.0 24.0 41.0 36-37 34.32580114423813 38.0 33.0 40.0 22.5 41.0 38-39 34.171016871259575 38.0 33.0 40.0 21.5 41.0 40-41 34.205372062659826 38.0 33.0 40.5 21.5 41.0 42-43 34.08819958757199 38.0 33.0 41.0 20.0 41.0 44-45 33.93364818665995 38.0 33.0 41.0 18.5 41.0 46-47 33.697471395333395 38.0 33.0 41.0 15.5 41.0 48-49 33.4322790018357 38.0 33.0 40.0 13.5 41.0 50-51 33.271444944154446 38.0 32.0 40.0 9.5 41.0 52-53 33.09668359299212 38.0 32.0 40.0 8.5 41.0 54-55 32.92503655190834 38.0 32.0 40.0 8.0 41.0 56-57 32.72174489881516 38.0 32.0 40.0 7.0 41.0 58-59 32.51875302026065 37.0 31.0 40.0 6.0 41.0 60-61 32.29103141482775 37.0 31.0 40.0 2.0 41.0 62-63 32.11922380781081 37.0 31.0 40.0 2.0 41.0 64-65 31.983008058660086 36.0 31.0 40.0 2.0 41.0 66-67 31.687537664805234 36.0 31.0 39.0 2.0 41.0 68-69 31.405859215583952 35.5 31.0 39.0 2.0 41.0 70-71 31.08870521587112 35.0 30.0 39.0 2.0 40.5 72-73 30.795026779001063 35.0 30.0 38.0 2.0 40.0 74-75 30.433593120779033 35.0 30.0 37.0 2.0 39.5 76-77 30.114062796718898 35.0 30.0 37.0 2.0 39.0 78-79 29.816303566364937 35.0 30.0 36.0 2.0 39.0 80-81 29.524966973337307 34.5 29.0 36.0 2.0 37.5 82-83 29.227906101865706 34.0 29.0 35.5 2.0 37.0 84-85 28.99140740672123 34.0 29.0 35.0 2.0 37.0 86-87 28.77288386194006 34.0 29.0 35.0 2.0 36.0 88-89 28.596399000168287 34.0 29.0 35.0 2.0 36.0 90-91 28.444059240595184 34.0 29.0 35.0 2.0 36.0 92-93 28.327206868104867 34.0 29.0 35.0 2.0 35.5 94-95 28.21462390260647 34.0 29.0 35.0 2.0 35.0 96-97 28.099194751552524 34.0 29.0 35.0 2.0 35.0 98-99 28.015214394118345 34.0 29.0 35.0 2.0 35.0 100-101 27.916231671939094 34.0 28.0 35.0 2.0 35.0 102-103 26.63308497077134 32.0 25.0 34.0 2.0 35.0 104-105 27.58215906628851 34.0 27.0 35.0 2.0 35.0 106-107 27.744068195233766 34.0 27.5 35.0 2.0 35.0 108-109 27.747997686203732 34.0 28.0 35.0 2.0 35.0 110-111 27.67374572017166 34.0 27.5 35.0 2.0 35.0 112-113 27.59168572100537 34.0 27.0 35.0 2.0 35.0 114-115 27.489227672715167 34.0 27.0 35.0 2.0 35.0 116-117 27.36813805788299 34.0 27.0 35.0 2.0 35.0 118-119 27.237183930849604 34.0 27.0 35.0 2.0 35.0 120-121 27.04379204241411 34.0 27.0 35.0 2.0 35.0 122-123 26.756208935391253 34.0 26.5 35.0 2.0 35.0 124-125 24.920481522823778 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5016208076387478 1101 2 0.6758935106980815 1101 3 0.4960558692120607 1101 4 0.4445025840734189 1101 5 0.37783053300177016 1101 6 0.5005831230001618 1101 7 0.5722492770110392 1101 8 0.7067646527052887 1101 9 0.9812646428702863 1101 10-11 0.7989517898862388 1101 12-13 0.814492634637979 1101 14-15 0.8556252238858022 1101 16-17 0.8244601622814685 1101 18-19 0.8930321784650417 1101 20-21 0.8606323312351734 1101 22-23 0.8584430680345037 1101 24-25 0.8327418794723158 1101 26-27 0.8966829995985321 1101 28-29 0.9104709457574387 1101 30-31 0.9131762117564435 1101 32-33 1.0235022664650941 1101 34-35 1.0372015513026582 1101 36-37 1.035646596212942 1101 38-39 0.9975955770215847 1101 40-41 0.8271593073103602 1101 42-43 0.855644070968772 1101 44-45 0.8104888718206027 1101 46-47 0.7728169811464554 1101 48-49 0.8605048235557788 1101 50-51 0.9971906405931605 1101 52-53 0.9322771073383365 1101 54-55 0.9971012938809238 1101 56-57 0.8498236807521451 1101 58-59 0.9312622688513201 1101 60-61 0.8429073199957315 1101 62-63 0.8372841357661898 1101 64-65 0.7710829620329278 1101 66-67 0.926824524690602 1101 68-69 1.0376379473576272 1101 70-71 0.9348404648724618 1101 72-73 0.9287677678843664 1101 74-75 1.02503509996955 1101 76-77 1.0482990148848792 1101 78-79 0.9304287786751644 1101 80-81 0.8320803354460331 1101 82-83 0.9492455767724088 1101 84-85 0.9580207134896241 1101 86-87 1.0105169768180176 1101 88-89 0.9690195605265721 1101 90-91 0.9214725453878536 1101 92-93 0.7949548937259792 1101 94-95 0.8978610154963178 1101 96-97 0.9788939595849229 1101 98-99 0.9613560921938031 1101 100-101 0.9100757955015801 1101 102-103 0.7809373470921663 1101 104-105 0.8874613601507555 1101 106-107 0.753903707304751 1101 108-109 0.7315560170255182 1101 110-111 0.7839874677191183 1101 112-113 0.8760658363536749 1101 114-115 0.9086156547365185 1101 116-117 1.0477630014396837 1101 118-119 1.030277704813514 1101 120-121 0.9234119740889319 1101 122-123 0.9396706941391137 1101 124-125 1.1831935849827993 1102 1 0.35072240251912845 1102 2 -0.3782328787118203 1102 3 0.14526949506111464 1102 4 0.2883008552330395 1102 5 0.4550329161598654 1102 6 0.2992426092617677 1102 7 0.3156252619774662 1102 8 0.3243669666244884 1102 9 0.687631575511098 1102 10-11 0.7287022303704802 1102 12-13 0.7584064647011672 1102 14-15 0.9562798715466343 1102 16-17 0.8643297606704934 1102 18-19 0.8318679396436508 1102 20-21 0.7050294629987235 1102 22-23 0.9516538999621602 1102 24-25 0.8608887085694974 1102 26-27 1.0645571167899277 1102 28-29 0.6911099182197518 1102 30-31 0.9218111496825543 1102 32-33 1.0706673296557412 1102 34-35 1.098677339770724 1102 36-37 0.6704672629063495 1102 38-39 0.1277450294810194 1102 40-41 -0.2904510015692239 1102 42-43 0.07314062298348034 1102 44-45 -0.07033469539366166 1102 46-47 0.16884975490024345 1102 48-49 -0.16933389144342215 1102 50-51 -0.2006853903125645 1102 52-53 -0.33943758577447625 1102 54-55 -0.2901786987687842 1102 56-57 -0.246053660819463 1102 58-59 -0.17608479517080156 1102 60-61 -0.29685803933839594 1102 62-63 -0.24052865627194464 1102 64-65 -0.16551129144335874 1102 66-67 -0.3159258228998141 1102 68-69 -0.2308051572047809 1102 70-71 -0.6944601258408909 1102 72-73 -0.4590464404038954 1102 74-75 -0.3802420053573279 1102 76-77 -0.7041527729659265 1102 78-79 -0.6083458908537835 1102 80-81 -0.6133558365973926 1102 82-83 -0.741683721889359 1102 84-85 -0.5944282570894259 1102 86-87 -0.3378999702644805 1102 88-89 -0.43099863752611256 1102 90-91 -0.3633503294374627 1102 92-93 -0.3095380963435197 1102 94-95 -0.19568687380876426 1102 96-97 -0.449483885796095 1102 98-99 -0.5670055856006613 1102 100-101 -0.3633240575941947 1102 102-103 -0.4297172954924946 1102 104-105 -0.277393707715035 1102 106-107 -0.16651978701577974 1102 108-109 -0.2342556288144877 1102 110-111 -0.20603685218894086 1102 112-113 0.015465204168044977 1102 114-115 -0.027313874890772638 1102 116-117 -0.23358054276940265 1102 118-119 0.1359335174542835 1102 120-121 0.18820366603067384 1102 122-123 0.06595337766406217 1102 124-125 0.24965188352324574 1103 1 0.13455426626331146 1103 2 -0.2941724892666784 1103 3 0.11644105526978521 1103 4 0.13834389884929976 1103 5 0.21533021015880394 1103 6 0.15045974267617623 1103 7 0.2982370618400054 1103 8 0.38826865921669906 1103 9 0.46633626331992417 1103 10-11 0.4272064995918683 1103 12-13 0.38043086699368445 1103 14-15 0.4418702186128982 1103 16-17 0.44822669093628775 1103 18-19 0.5541086129559218 1103 20-21 0.5585272864607731 1103 22-23 0.6002948735775533 1103 24-25 0.46867681219183766 1103 26-27 0.6562829615573662 1103 28-29 0.6159344263642907 1103 30-31 0.5860409404319711 1103 32-33 0.6229325300908144 1103 34-35 0.6219238737637625 1103 36-37 0.7708345608201839 1103 38-39 0.71444207828484 1103 40-41 0.5996237469116892 1103 42-43 0.6052148406819313 1103 44-45 0.5139342519799683 1103 46-47 0.5479091814775785 1103 48-49 0.5333592333252142 1103 50-51 0.5278183004269863 1103 52-53 0.5394074473118948 1103 54-55 0.532541607034986 1103 56-57 0.4930592093613413 1103 58-59 0.6610281008894603 1103 60-61 0.6818535750293719 1103 62-63 0.5206787951831515 1103 64-65 0.4455207506153158 1103 66-67 0.41669365199597763 1103 68-69 0.4532361144302506 1103 70-71 0.3448564054703276 1103 72-73 0.38577872233852517 1103 74-75 0.48613059124723534 1103 76-77 0.5527442910103595 1103 78-79 0.423389293350823 1103 80-81 0.4444832140225614 1103 82-83 0.44882569987997556 1103 84-85 0.5000531783371542 1103 86-87 0.612525277321943 1103 88-89 0.5511283032107066 1103 90-91 0.5052115545459728 1103 92-93 0.3666231361881671 1103 94-95 0.32000197247770146 1103 96-97 0.3215077107402209 1103 98-99 0.32849040042037103 1103 100-101 0.30262050050275846 1103 102-103 0.27157713375270376 1103 104-105 0.2473020121028675 1103 106-107 0.36729158882970836 1103 108-109 0.3958365020310026 1103 110-111 0.3338279048448811 1103 112-113 0.29339160554665966 1103 114-115 0.34055649493926765 1103 116-117 0.29612259709304567 1103 118-119 0.14843310198760662 1103 120-121 0.25007673950218035 1103 122-123 0.268511903915595 1103 124-125 0.6435308478682167 1104 1 0.4651684885157117 1104 2 0.557520076955786 1104 3 0.3054439866723051 1104 4 0.1988836406380159 1104 5 0.39449334424049454 1104 6 0.4584607122921085 1104 7 0.6005144721867381 1104 8 0.5401213402736005 1104 9 0.622760669246162 1104 10-11 0.6872890570227383 1104 12-13 0.6460497794426274 1104 14-15 0.7250265606347099 1104 16-17 0.6643590439856979 1104 18-19 0.720945587611773 1104 20-21 0.6174674824779913 1104 22-23 0.7613770091162806 1104 24-25 0.8283400415969382 1104 26-27 0.7220889951610729 1104 28-29 0.9705903278566268 1104 30-31 0.9183103830238935 1104 32-33 1.0086242729469603 1104 34-35 1.1215990856654514 1104 36-37 1.123683234970109 1104 38-39 1.2388641014820223 1104 40-41 1.154194795139162 1104 42-43 1.0981880929100356 1104 44-45 1.0931354798119273 1104 46-47 1.1393724658212463 1104 48-49 1.0139896474988959 1104 50-51 0.9843736781035517 1104 52-53 0.938277087388272 1104 54-55 0.6367348398090513 1104 56-57 0.9005769854555652 1104 58-59 0.8953087011871403 1104 60-61 1.0608365324386853 1104 62-63 0.9391893900890622 1104 64-65 1.0048173707060712 1104 66-67 1.017731715594138 1104 68-69 0.7185476890186244 1104 70-71 0.3382659136419015 1104 72-73 0.3534970289674533 1104 74-75 0.6953044542574105 1104 76-77 0.9286139835561649 1104 78-79 1.0065393200484998 1104 80-81 0.9245883997821238 1104 82-83 0.8338923206141331 1104 84-85 0.8714008851266293 1104 86-87 0.8933710654162255 1104 88-89 0.9344233093325336 1104 90-91 0.8975700004729106 1104 92-93 0.9355829738131121 1104 94-95 0.9704868279268162 1104 96-97 0.8765287851667907 1104 98-99 0.91454280526494 1104 100-101 0.9405056907590215 1104 102-103 0.8453575105211435 1104 104-105 0.9068049604807875 1104 106-107 0.9040397714527373 1104 108-109 0.8787702288770873 1104 110-111 0.8663694524364232 1104 112-113 0.8617400319506956 1104 114-115 0.9017012970120213 1104 116-117 0.8877524896589364 1104 118-119 0.8597823686146562 1104 120-121 0.7696037929500044 1104 122-123 0.7789476802742961 1104 124-125 1.2364837533565414 1105 1 0.14756126965306038 1105 2 -0.04558148196421641 1105 3 0.1962875351691622 1105 4 0.3092645414583117 1105 5 -0.1541089556896651 1105 6 0.19159950868537123 1105 7 0.3540107836260731 1105 8 0.382351556719172 1105 9 0.5265849598401289 1105 10-11 0.4880258981989769 1105 12-13 0.41467989657588333 1105 14-15 0.4864295352197274 1105 16-17 0.39617247618541995 1105 18-19 0.5075542880302208 1105 20-21 0.46123458952371266 1105 22-23 0.47870729851320704 1105 24-25 0.3892093053874959 1105 26-27 0.5177291705694671 1105 28-29 0.36320911883370144 1105 30-31 0.451076653718701 1105 32-33 0.5299182989488642 1105 34-35 0.5479089294064821 1105 36-37 0.607308083394571 1105 38-39 0.675499081574273 1105 40-41 0.37426528923187163 1105 42-43 0.43343067996334383 1105 44-45 0.4826775283351523 1105 46-47 0.34905854042765583 1105 48-49 0.35306682566346836 1105 50-51 0.30147440647901647 1105 52-53 0.39943176957145 1105 54-55 0.23652467209526407 1105 56-57 0.31070735261152294 1105 58-59 0.20368122177597314 1105 60-61 0.35962748164006086 1105 62-63 0.4084902485738908 1105 64-65 0.2623896444541103 1105 66-67 0.5223594000866214 1105 68-69 0.5792495664998292 1105 70-71 0.6605737451240508 1105 72-73 0.6342536536400623 1105 74-75 0.5989748757935978 1105 76-77 0.6114545526835506 1105 78-79 0.39582664270134416 1105 80-81 0.4273955520339854 1105 82-83 0.49560179074948607 1105 84-85 0.387937570180938 1105 86-87 0.510796730403456 1105 88-89 0.4591383380311491 1105 90-91 0.2977890519473938 1105 92-93 0.47678858491249443 1105 94-95 0.4181967221601326 1105 96-97 0.4238563525329866 1105 98-99 0.34809709456524374 1105 100-101 0.30808917314211826 1105 102-103 0.016879065311652397 1105 104-105 0.21460386883498472 1105 106-107 0.22190955479813823 1105 108-109 0.27696157648900765 1105 110-111 0.23281186511254148 1105 112-113 0.20863730014202275 1105 114-115 0.22572375237199438 1105 116-117 -0.07701683797856873 1105 118-119 0.05032875821947158 1105 120-121 0.10703782376887361 1105 122-123 0.232976773487362 1105 124-125 0.6224439044060226 1106 1 -0.18718880844698305 1106 2 -0.44415483496623764 1106 3 -0.029530156793960316 1106 4 -0.27157436012439007 1106 5 0.2500452293081281 1106 6 0.28834395702750015 1106 7 0.4158492815114272 1106 8 0.503613040117898 1106 9 0.49660390634338825 1106 10-11 0.41439537619806543 1106 12-13 0.5028854261644184 1106 14-15 0.5907211575692273 1106 16-17 0.45056966966365763 1106 18-19 0.5065273119410634 1106 20-21 0.47309853942076074 1106 22-23 0.4171009061196145 1106 24-25 0.5101159003086408 1106 26-27 0.6311670719331772 1106 28-29 0.7221257913234922 1106 30-31 0.6170821182053317 1106 32-33 0.5364176038613806 1106 34-35 0.6039907897243921 1106 36-37 0.5341494389983268 1106 38-39 0.5034608641116733 1106 40-41 0.6305103373846919 1106 42-43 0.38728587258925273 1106 44-45 0.4307028700879556 1106 46-47 0.33611740099920695 1106 48-49 0.5853398711461963 1106 50-51 0.5910334985123029 1106 52-53 0.42476977685157635 1106 54-55 0.5099557683085507 1106 56-57 0.15670524177418343 1106 58-59 0.5452463408268997 1106 60-61 0.5331094233529043 1106 62-63 0.5132505882414904 1106 64-65 0.5544656121250782 1106 66-67 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-1.1147085753724824 2216 54-55 -1.2079276396871457 2216 56-57 -0.9906521731811964 2216 58-59 -1.1307912323833378 2216 60-61 -1.0414047429762192 2216 62-63 -1.2418829620050715 2216 64-65 -1.0329281641317678 2216 66-67 -0.9329532738164019 2216 68-69 -0.794043270956184 2216 70-71 -0.88307663595387 2216 72-73 -0.757268801734206 2216 74-75 -0.7758872466189963 2216 76-77 -0.9737979131791725 2216 78-79 -0.8799188722189086 2216 80-81 -0.8900439660626844 2216 82-83 -0.8412036776678313 2216 84-85 -0.7764098251725144 2216 86-87 -0.9185178770721478 2216 88-89 -0.9798868051842788 2216 90-91 -0.9209423627115072 2216 92-93 -0.9312313296103696 2216 94-95 -0.75407412817761 2216 96-97 -0.8833321735819943 2216 98-99 -0.9200490698186989 2216 100-101 -0.9752601239162111 2216 102-103 -0.8105811010616328 2216 104-105 -0.7183473907158664 2216 106-107 -0.8690618184486922 2216 108-109 -0.8761468784494717 2216 110-111 -0.9416894751233365 2216 112-113 -0.8977339888147426 2216 114-115 -0.9172048153860999 2216 116-117 -0.9453303974052574 2216 118-119 -0.8843021779857771 2216 120-121 -0.9768409451341498 2216 122-123 -0.8527173089599493 2216 124-125 -1.1736167203044303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54928.0 3 18354.0 4 8895.0 5 6833.0 6 7373.0 7 8601.0 8 9894.0 9 10900.0 10 12072.0 11 13396.0 12 14831.0 13 17090.0 14 19800.0 15 17719.0 16 12312.0 17 10598.0 18 10009.0 19 9394.0 20 9502.0 21 9670.0 22 10901.0 23 13431.0 24 17407.0 25 22853.0 26 28783.0 27 32818.0 28 37462.0 29 44994.0 30 52337.0 31 61247.0 32 74965.0 33 93914.0 34 121459.0 35 167239.0 36 271660.0 37 435219.0 38 173488.0 39 779.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.56904592998785 19.68854179385213 13.883784297573031 23.85862797858699 2 15.2173275344329 20.866366428957036 39.658498904086045 24.25780713252402 3 19.410825952189434 25.397722331067396 29.221713248799485 25.96973846794368 4 12.64828853853493 17.022452692942526 36.990636468728816 33.33862229979373 5 13.009237172866209 38.95761831316224 34.06560662272718 13.967537891244369 6 31.58316466190836 37.38391777789099 17.24730292976218 13.785614630438465 7 27.916804120474897 32.90554105678776 20.882034011004198 18.295620811733144 8 25.908590974384204 36.437772680226935 19.1904089643697 18.463227381019166 9 26.29591564177295 16.05689414615292 19.667563368256147 37.97962684381798 10-11 24.89500736575983 27.283546372122046 27.128743189084524 20.6927030730336 12-13 25.695875771372638 24.4793829739384 27.92105199505745 21.903689259631513 14-15 22.60832422000643 25.608542939852043 26.005558057253136 25.77757478288839 16-17 22.0067010804019 29.0945821911622 26.526134908326533 22.372581820109367 18-19 21.84394505934783 27.303220122089765 29.21683297921314 21.63600183934926 20-21 22.710077811409683 26.557212447869905 29.287598061958352 21.445111678762057 22-23 22.31472775582862 26.554877782049243 29.09848404144454 22.031910420677598 24-25 22.003991504415303 26.660506492884924 29.308737926033658 22.026764076666115 26-27 21.977410637595998 26.950605904812193 29.05309328726326 22.018890170328547 28-29 21.835320079438965 26.892838193283303 29.328448423597635 21.9433933036801 30-31 22.013383582236262 26.65664673487631 29.257892047200208 22.072077635687222 32-33 21.8902830334816 26.687730652705667 29.179693349945733 22.242292963867005 34-35 22.129588030015537 26.741998850306747 29.116624904085015 22.011788215592702 36-37 22.17809232232376 26.636910505592276 28.9588122649729 22.22618490711106 38-39 22.07333848997003 26.809982054698434 29.079056592801187 22.03762286253034 40-41 22.286422863765466 26.636833310432102 28.979912275419977 22.09683155038245 42-43 22.05249579672353 26.682429918373842 28.8512022116928 22.41387207320983 44-45 22.199346723091182 26.839959508565318 28.626512832151473 22.334180936192023 46-47 22.297101527589295 27.012670813590674 28.19185261694166 22.498375041878376 48-49 22.471819906779125 27.145781001447666 27.937314442133736 22.445084649639472 50-51 22.4474262361647 27.268598501281698 27.862486600206783 22.421488662346825 52-53 22.630147180292383 27.270271063085428 27.632496486333626 22.46708527028856 54-55 22.41955878334252 27.14894600301473 27.76202996510264 22.66946524854011 56-57 22.33074843369699 27.443744077951525 27.663487138893363 22.562020349458116 58-59 22.42279706830727 27.106990110438968 27.737911836672097 22.73230098458167 60-61 22.525599201699116 27.105639518158103 27.90566442646312 22.463096853679662 62-63 22.448404041526878 27.18504760625528 27.939913345859534 22.42663500635831 64-65 22.293948165361765 27.044526037100614 27.903659138144477 22.757866659393148 66-67 22.287838108368625 27.4420045627486 27.584043657465518 22.686113671417257 68-69 22.19160718563614 27.27995320942821 27.807839582240273 22.720600022695383 70-71 22.157481214557773 27.189190413184523 27.851602082622495 22.801726289635212 72-73 22.298413845312222 27.235816289928554 27.647472347407042 22.818297517352185 74-75 22.258415716490948 27.347357104387797 27.551952274336106 22.84227490478515 76-77 22.411067315723574 27.115469035220034 27.65326198441996 22.820201664636432 78-79 22.3590892412076 27.292812049855726 27.535565096877352 22.812533612059323 80-81 22.163662394847055 27.40249968864539 27.592683320964923 22.841154595542637 82-83 22.295094453424817 27.369363917026522 27.515803135873263 22.819738493675402 84-85 22.358806192286966 27.264121181991708 27.429112970999835 22.947959654721487 86-87 22.235422594611677 27.102217199390466 27.70189493532847 22.96046527066939 88-89 22.32496490137389 27.097760481211424 27.589038318230287 22.988236299184393 90-91 22.249343455162734 27.320885356438357 27.615950990336707 22.813820198062196 92-93 22.25788712957452 27.131962473106576 27.527904702132407 23.082245695186497 94-95 22.115692901184534 27.075250356770297 27.568964869511873 23.24009187253329 96-97 22.224678417804014 26.95528537404156 27.62635860998774 23.19367759816669 98-99 22.04422767039434 26.887367559135516 27.53699492083784 23.531409849632304 100-101 21.904933208994397 27.02642614082002 27.74670162155263 23.32193902863295 102-103 22.071588733006127 26.918595645060773 27.567652551788946 23.442163070144154 104-105 22.206062702026166 26.787775580288887 27.676652117952145 23.329509599732802 106-107 21.87348663140684 26.960592332145712 27.682917854417095 23.483003182030355 108-109 21.983772029252858 26.933655631787225 27.38141340772204 23.701158931237877 110-111 21.82495489217867 26.894065323741895 27.656060710547255 23.624919073532183 112-113 21.787098836051374 26.94381273071704 27.590991221881016 23.678097211350572 114-115 21.819109095802798 26.962802740119407 27.480550679394604 23.737537484683195 116-117 21.634355345790574 26.85774012712499 27.593410003566415 23.91449452351802 118-119 21.631627783464488 26.888412337433426 27.463567744156713 24.016392134945374 120-121 21.583365819955922 26.87769462060128 27.6058581421769 23.9330814172659 122-123 21.658720782567727 26.869165552218572 27.475329245751308 23.996784419462394 124-125 21.47768646209103 26.75677534151955 27.450489548058176 24.315048648331246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202.0 1 166.0 2 215.5 3 339.0 4 265.5 5 194.0 6 405.0 7 943.0 8 1606.5 9 1850.0 10 1490.0 11 1094.5 12 1017.0 13 1110.5 14 1243.0 15 1421.5 16 1630.5 17 1852.5 18 2176.5 19 2470.5 20 2792.5 21 3335.0 22 4025.5 23 4938.5 24 6043.0 25 7719.5 26 10154.0 27 13244.0 28 16166.5 29 19552.0 30 23827.0 31 27406.0 32 30887.5 33 35085.0 34 39676.5 35 43822.5 36 48360.0 37 52740.5 38 55956.0 39 58299.5 40 59963.0 41 62221.0 42 64790.5 43 67377.5 44 69432.5 45 70802.5 46 71129.0 47 70975.0 48 73055.5 49 74690.0 50 73686.5 51 71428.5 52 69157.0 53 67527.0 54 64612.5 55 59187.5 56 52902.5 57 47253.0 58 43640.0 59 40555.0 60 35460.5 61 28363.0 62 21020.0 63 15485.5 64 11328.0 65 8201.5 66 5969.0 67 4552.5 68 3533.0 69 2444.5 70 1674.5 71 1293.0 72 991.0 73 860.5 74 670.0 75 460.0 76 330.0 77 178.0 78 95.0 79 58.0 80 40.5 81 34.5 82 22.5 83 16.5 84 10.5 85 5.0 86 2.0 87 3.0 88 3.5 89 3.0 90 3.5 91 3.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10622054039699927 2 0.0 3 0.0 4 0.10184614798723912 5 0.0 6 0.0 7 0.002573172005741261 8 0.007513662256764484 9 0.004117075209186019 10-11 7.462198816649658E-4 12-13 0.0 14-15 1.0292688022965045E-4 16-17 3.8597580086118926E-4 18-19 0.0024959768455690232 20-21 0.0016210983636169947 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.00905756546020924 58-59 9.006102020094415E-4 60-61 0.0 62-63 0.0 64-65 2.8304892063153877E-4 66-67 0.0 68-69 2.5731720057412612E-5 70-71 0.0 72-73 0.0 74-75 0.0011064639624687425 76-77 0.0 78-79 0.0 80-81 2.5731720057412617E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0015953666435595821 90-91 0.0 92-93 0.0020842693246504217 94-95 0.0 96-97 0.010009639102333507 98-99 0.0046317096103342704 100-101 0.0026246354458560866 102-103 0.0 104-105 0.0 106-107 0.0014409763232151064 108-109 2.5731720057412612E-5 110-111 2.5731720057412617E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.001003537082239092 122-123 0.004503051010047208 124-125 0.08715333583445653 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1943127.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.76439604384952 #Duplication Level Percentage of deduplicated Percentage of total 1 78.19560081543344 45.95117255204893 2 12.181795587792378 14.317117208924998 3 4.172839650046021 7.356432054683484 4 1.8406290814630817 4.32653825251694 5 0.9531390528431186 2.8005320393066317 6 0.582032137742214 2.05216602315191 7 0.3999909144454753 1.6453657157290809 8 0.26536602646379404 1.2475259420560836 9 0.196946175972897 1.0416080775773697 >10 1.0986981201970594 11.701564112885286 >50 0.07684687159585284 3.0964408997223485 >100 0.03558856071390859 3.6671654902083723 >500 1.75668430264854E-4 0.08165531923911826 >1k 2.63502645397281E-4 0.44843325761659886 >5k 8.7834215132427E-5 0.2662830543328768 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5159 0.26549988755238335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4489 0.23101938267545047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2851 0.14672226776736672 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.1463440114825225E-5 1.0292688022965045E-4 14-15 0.0 0.0 0.0 5.1463440114825225E-5 1.0292688022965045E-4 16-17 0.0 0.0 0.0 5.1463440114825225E-5 1.0292688022965045E-4 18-19 0.0 0.0 0.0 1.2865860028706306E-4 1.0292688022965045E-4 20-21 0.0 0.0 0.0 1.5439032034447568E-4 1.0292688022965045E-4 22-23 0.0 0.0 0.0 3.0878064068895136E-4 1.0292688022965045E-4 24-25 0.0 0.0 0.0 4.117075209186018E-4 1.0292688022965045E-4 26-27 0.0 0.0 0.0 6.432930014353154E-4 1.5439032034447568E-4 28-29 0.0 0.0 0.0 0.001415244603157694 1.5439032034447568E-4 30-31 0.0 0.0 0.0 0.0032679284472914016 1.5439032034447568E-4 32-33 0.0 0.0 0.0 0.007951101497740497 1.5439032034447568E-4 34-35 0.0 0.0 0.0 0.013895128831002811 1.5439032034447568E-4 36-37 0.0 0.0 0.0 0.024856841575460586 1.5439032034447568E-4 38-39 0.0 0.0 0.0 0.04431002193886452 1.5439032034447568E-4 40-41 5.1463440114825225E-5 0.0 0.0 0.06777735063122482 1.5439032034447568E-4 42-43 1.0292688022965045E-4 0.0 0.0 0.08877443419807352 1.5439032034447568E-4 44-45 1.0292688022965045E-4 0.0 0.0 0.11273066557152467 1.5439032034447568E-4 46-47 1.0292688022965045E-4 0.0 0.0 0.14368592480059203 1.5439032034447568E-4 48-49 1.0292688022965045E-4 0.0 0.0 0.1809454554437255 2.058537604593009E-4 50-51 1.0292688022965045E-4 0.0 0.0 0.2219360854951838 2.058537604593009E-4 52-53 1.0292688022965045E-4 0.0 0.0 0.26290098382658467 2.058537604593009E-4 54-55 1.0292688022965045E-4 0.0 0.0 0.3057957611622915 2.058537604593009E-4 56-57 1.0292688022965045E-4 0.0 0.0 0.3588288362006189 2.3158548051671352E-4 58-59 1.0292688022965045E-4 0.0 0.0 0.4206878912186388 2.830489206315387E-4 60-61 1.0292688022965045E-4 0.0 0.0 0.48800207088883024 3.0878064068895136E-4 62-63 1.0292688022965045E-4 0.0 0.0 0.5558308849601699 3.602440808037766E-4 64-65 1.0292688022965045E-4 0.0 0.0 0.6287545796028772 3.602440808037766E-4 66-67 1.0292688022965045E-4 0.0 0.0 0.7125113283897553 3.602440808037766E-4 68-69 1.0292688022965045E-4 0.0 0.0 0.8095971081663731 3.602440808037766E-4 70-71 1.0292688022965045E-4 0.0 0.0 0.9199347237725584 3.602440808037766E-4 72-73 1.0292688022965045E-4 0.0 0.0 1.0372456355143025 3.602440808037766E-4 74-75 1.0292688022965045E-4 0.0 0.0 1.1503108134465734 3.859758008611892E-4 76-77 1.0292688022965045E-4 0.0 0.0 1.2758816073267472 4.6317096103342704E-4 78-79 1.2865860028706306E-4 0.0 0.0 1.4254343643004292 4.6317096103342704E-4 80-81 2.058537604593009E-4 0.0 0.0 1.5960099365610172 4.6317096103342704E-4 82-83 2.058537604593009E-4 0.0 0.0 1.7796572226107712 4.6317096103342704E-4 84-85 2.058537604593009E-4 0.0 0.0 1.9579523108885832 4.6317096103342704E-4 86-87 2.058537604593009E-4 0.0 0.0 2.1475436242715995 5.146344011482523E-4 88-89 2.058537604593009E-4 0.0 0.0 2.3642047071550136 5.660978412630775E-4 90-91 2.058537604593009E-4 0.0 0.0 2.6158351975964518 5.660978412630775E-4 92-93 2.058537604593009E-4 0.0 0.0 2.886893136681236 5.660978412630775E-4 94-95 2.058537604593009E-4 0.0 0.0 3.160472784331647 5.660978412630775E-4 96-97 2.058537604593009E-4 0.0 0.0 3.4325085287786132 6.175612813779027E-4 98-99 2.830489206315387E-4 0.0 0.0 3.7357053862151055 6.175612813779027E-4 100-101 3.34512360746364E-4 0.0 0.0 4.088796048842922 6.175612813779027E-4 102-103 3.602440808037766E-4 0.0 0.0 4.474154288422733 6.175612813779027E-4 104-105 3.602440808037766E-4 0.0 0.0 4.854855086672153 6.175612813779027E-4 106-107 4.117075209186018E-4 0.0 0.0 5.238309178967715 6.175612813779027E-4 108-109 4.117075209186018E-4 0.0 0.0 5.637716937698874 6.175612813779027E-4 110-111 4.117075209186018E-4 0.0 0.0 6.088073502143709 6.175612813779027E-4 112-113 4.117075209186018E-4 0.0 0.0 6.586239602455218 6.175612813779027E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4035 0.0 64.10045 1 GTATCAA 7200 0.0 49.08367 1 ATCAACG 8730 0.0 40.040825 3 TCAACGC 8820 0.0 39.49721 4 CAACGCA 9005 0.0 38.852146 5 TATCAAC 9150 0.0 38.00764 2 AACGCAG 9270 0.0 37.869854 6 GTACATG 9455 0.0 31.389378 1 ACGCAGA 11185 0.0 31.173317 7 CGCAGAG 11225 0.0 31.009224 8 TACATGG 9740 0.0 29.591402 2 ACATGGG 9520 0.0 29.086748 3 TGGTATC 2075 0.0 28.98561 2 GCAGAGT 12350 0.0 27.653822 9 GAGTACT 6800 0.0 26.86139 12-13 GTGGTAT 2440 0.0 25.88993 1 GTATACG 165 1.0965936E-5 25.28302 1 CAGAGTA 11895 0.0 24.533348 10-11 GTACTTT 7475 0.0 24.515375 14-15 CATGGGA 6545 0.0 23.74707 4 >>END_MODULE