FastQCFastQC Report
Thu 26 May 2016
SRR1364577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118205
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41730.13382699341448046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.058.074231
GTATCAA86250.056.846241
ATCAACG108450.044.8248863
TCAACGC108900.044.4211044
CAACGCA111300.043.570165
AACGCAG113200.042.779436
TATCAAC116350.041.8836142
ACGCAGA134750.035.6294177
GTACATG153150.035.083621
CGCAGAG134850.034.6340758
TACATGG156650.033.311632
ACATGGG162900.031.3030343
GCAGAGT150250.029.5809369
CATGGGG84000.026.9881044
AGAGTAC141200.024.6539310-11
GAGTACT62000.023.7571212-13
CATGGGA88100.022.6253644
CAGAGTA150500.022.47806710-11
AGTACTT68000.021.09203112-13
GTACTTT70950.020.42475314-15
Produced by FastQC (version 0.11.5)