##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364577_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3118205 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.147216427399737 31.0 30.0 34.0 23.0 34.0 2 29.11232167224413 31.0 30.0 34.0 23.0 34.0 3 29.208597253868813 31.0 30.0 34.0 23.0 34.0 4 32.61677214936157 35.0 33.0 37.0 25.0 37.0 5 32.66947169926288 35.0 33.0 37.0 25.0 37.0 6 32.69913908803302 35.0 33.0 37.0 25.0 37.0 7 32.454664783104384 35.0 33.0 37.0 23.0 37.0 8 32.42891695703137 35.0 33.0 37.0 23.0 37.0 9 33.58758516518317 38.0 33.0 39.0 21.0 39.0 10-11 33.56350865321555 38.0 33.0 39.0 20.5 39.0 12-13 33.51914595095576 38.0 33.0 39.0 19.0 39.0 14-15 34.322335606542865 39.0 33.0 41.0 17.5 41.0 16-17 34.439490508160944 39.0 33.0 41.0 18.0 41.0 18-19 34.30039028864363 39.0 33.0 41.0 17.5 41.0 20-21 34.07679594510303 39.0 33.0 41.0 18.0 41.0 22-23 33.92900498844688 39.0 32.5 41.0 17.5 41.0 24-25 33.79808383348754 38.5 32.0 41.0 17.0 41.0 26-27 33.72125549795476 38.0 32.0 41.0 16.5 41.0 28-29 33.74396648071567 38.0 32.5 40.5 16.5 41.0 30-31 33.714436991794955 38.0 32.5 40.0 16.5 41.0 32-33 33.67547787910031 38.0 33.0 40.0 16.0 41.0 34-35 33.32735484036489 38.0 32.0 40.0 14.0 41.0 36-37 33.144046655046736 38.0 32.0 40.0 11.0 41.0 38-39 32.98345682852795 38.0 31.0 40.0 9.0 41.0 40-41 33.132285882422735 38.0 32.0 40.0 10.5 41.0 42-43 33.008936391289225 38.0 32.0 40.0 9.0 41.0 44-45 32.85418085084207 38.0 32.0 40.0 8.5 41.0 46-47 32.78502359530563 38.0 32.0 40.0 7.5 41.0 48-49 32.61748778544066 38.0 31.5 40.0 4.5 41.0 50-51 32.46057315025792 38.0 31.0 40.0 2.0 41.0 52-53 32.40666777841739 37.5 31.0 40.0 2.0 41.0 54-55 32.42464286344227 38.0 31.0 40.0 2.0 41.0 56-57 32.29072334884974 37.0 31.0 40.0 2.0 41.0 58-59 32.08938235298834 37.0 31.0 40.0 2.0 41.0 60-61 31.882630231174666 37.0 31.0 40.0 2.0 41.0 62-63 31.601759185172238 36.0 30.5 40.0 2.0 41.0 64-65 31.323177437019055 36.0 30.0 39.5 2.0 41.0 66-67 31.070646253212985 35.0 30.0 39.0 2.0 41.0 68-69 31.02799815919736 35.0 30.0 39.0 2.0 41.0 70-71 30.78765924626508 35.0 30.0 39.0 2.0 40.5 72-73 30.502039314284982 35.0 30.0 38.0 2.0 40.0 74-75 30.190472403193503 35.0 30.0 37.0 2.0 39.5 76-77 29.87899143898493 35.0 30.0 37.0 2.0 39.0 78-79 29.5593389466055 35.0 29.5 36.0 2.0 39.0 80-81 29.25065318027519 34.5 29.0 36.0 2.0 37.5 82-83 28.990342360428514 34.0 29.0 35.5 2.0 37.0 84-85 28.739477199221987 34.0 29.0 35.0 2.0 37.0 86-87 28.54064213225237 34.0 29.0 35.0 2.0 36.0 88-89 28.33938852641183 34.0 29.0 35.0 2.0 36.0 90-91 28.19338353315449 34.0 29.0 35.0 2.0 36.0 92-93 28.066558164071957 34.0 29.0 35.0 2.0 35.5 94-95 27.940773137109332 34.0 28.5 35.0 2.0 35.0 96-97 27.824349104693244 34.0 28.0 35.0 2.0 35.0 98-99 27.73745167492195 34.0 28.0 35.0 2.0 35.0 100-101 27.653182520071645 34.0 27.5 35.0 2.0 35.0 102-103 26.482959266629358 32.5 25.0 34.0 2.0 35.0 104-105 27.365511889051554 34.0 27.0 35.0 2.0 35.0 106-107 27.50748635833757 34.0 27.0 35.0 2.0 35.0 108-109 27.463907761035596 34.0 27.0 35.0 2.0 35.0 110-111 27.38575061613973 34.0 27.0 35.0 2.0 35.0 112-113 27.29862436882758 34.0 27.0 35.0 2.0 35.0 114-115 27.180500159546916 34.0 27.0 35.0 2.0 35.0 116-117 27.069559249632402 34.0 27.0 35.0 2.0 35.0 118-119 26.932194323336663 34.0 26.5 35.0 2.0 35.0 120-121 26.756047950663923 34.0 25.5 35.0 2.0 35.0 122-123 26.526098508597094 34.0 25.0 35.0 2.0 35.0 124-125 25.05293766766457 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7997674978223124 1101 2 0.8297201789658999 1101 3 0.7627803766995989 1101 4 0.8480392576239453 1101 5 0.768163469986689 1101 6 0.7780247391123538 1101 7 0.893597383380822 1101 8 0.7997459494672512 1101 9 1.099514894375524 1101 10-11 1.1696574157957969 1101 12-13 1.034421755122544 1101 14-15 1.241110936169072 1101 16-17 1.1023173157723605 1101 18-19 1.4403582466383185 1101 20-21 1.7592792411429556 1101 22-23 1.6280462897720014 1101 24-25 1.7653416714708499 1101 26-27 1.941390758492247 1101 28-29 1.7619843172959833 1101 30-31 1.7598290471163338 1101 32-33 1.711766692062838 1101 34-35 2.045216176362999 1101 36-37 1.9217534560541694 1101 38-39 1.7980789973327163 1101 40-41 1.6281940774209787 1101 42-43 1.6978982489075136 1101 44-45 1.6225762780336126 1101 46-47 1.4113647957487316 1101 48-49 1.336013710831594 1101 50-51 1.326378533569006 1101 52-53 1.1274073517275482 1101 54-55 1.0013857473350924 1101 56-57 0.9740434600196366 1101 58-59 1.0369904724975285 1101 60-61 1.0435606888835913 1101 62-63 1.05038236792846 1101 64-65 1.151637281102598 1101 66-67 1.0527502700682057 1101 68-69 0.9681440008879747 1101 70-71 0.8816980403776 1101 72-73 0.7039395236840491 1101 74-75 0.6885767986226128 1101 76-77 0.8044834500605162 1101 78-79 0.8624229137906916 1101 80-81 0.9235979841242248 1101 82-83 0.7964784464486385 1101 84-85 0.8126975922544055 1101 86-87 0.8893216659170058 1101 88-89 0.8188694040285789 1101 90-91 0.792861999957438 1101 92-93 0.6753093440753304 1101 94-95 0.7712217221573958 1101 96-97 0.7896199963650155 1101 98-99 0.8463820834186286 1101 100-101 0.9265394085712053 1101 102-103 0.8317392608073106 1101 104-105 0.8659760861587422 1101 106-107 0.8232428014769297 1101 108-109 0.8415249693314024 1101 110-111 0.7211555233620892 1101 112-113 0.8122890141783969 1101 114-115 0.8949438852519513 1101 116-117 1.0337069337249538 1101 118-119 0.9909788800453896 1101 120-121 1.0926894583130888 1101 122-123 1.1128588475385577 1101 124-125 1.3849933281309248 1102 1 -0.06832743724733348 1102 2 0.19510480932551744 1102 3 0.3219108926024461 1102 4 0.43264023688606557 1102 5 0.4065983637260473 1102 6 -0.32011701763823197 1102 7 -0.7836763469992469 1102 8 -0.03358423013058598 1102 9 0.2468256846518031 1102 10-11 0.421650810238404 1102 12-13 0.5703611018585519 1102 14-15 0.6836340289105394 1102 16-17 0.5486156440433589 1102 18-19 0.7548566539629533 1102 20-21 1.0337639674851502 1102 22-23 0.8448587913975345 1102 24-25 1.0510617557199708 1102 26-27 0.5671818594138003 1102 28-29 1.166196356872021 1102 30-31 0.8455476362612231 1102 32-33 0.6407587410673727 1102 34-35 1.0229080920696418 1102 36-37 1.0973753298651872 1102 38-39 1.218476747231989 1102 40-41 0.939796596328982 1102 42-43 0.8088347884295786 1102 44-45 0.8008310342950224 1102 46-47 0.45218139442708605 1102 48-49 0.25689133468112857 1102 50-51 0.5955908565918833 1102 52-53 0.2804608023268855 1102 54-55 0.29011488459609325 1102 56-57 0.32023708427222886 1102 58-59 0.5101222618865435 1102 60-61 0.38515542701997063 1102 62-63 0.26201818794719145 1102 64-65 0.3081817222193983 1102 66-67 0.25431880183068145 1102 68-69 0.13257161269101303 1102 70-71 0.10632277310825344 1102 72-73 0.27731955099150696 1102 74-75 -0.08148496942847316 1102 76-77 0.14982180850147842 1102 78-79 0.11936872420493216 1102 80-81 -0.24028152013035609 1102 82-83 -0.14156900893846114 1102 84-85 -0.5450624347945201 1102 86-87 -0.12872015401731218 1102 88-89 0.13363445774253435 1102 90-91 -0.36937065087638743 1102 92-93 0.037818118078956076 1102 94-95 -0.021271826701443786 1102 96-97 0.13448424323662778 1102 98-99 0.16889216641715876 1102 100-101 0.18917477196018595 1102 102-103 0.007895673317158014 1102 104-105 0.3100873767342769 1102 106-107 0.2680654151465127 1102 108-109 -0.1676774166992132 1102 110-111 0.06041429740465176 1102 112-113 -0.1918428854745713 1102 114-115 -0.1924462226400685 1102 116-117 -0.25674143713316155 1102 118-119 0.14822216558678036 1102 120-121 0.5446004234542663 1102 122-123 0.31304084533109844 1102 124-125 0.6471302066367315 1103 1 0.42227435522569223 1103 2 0.527446435907386 1103 3 0.37664735428504414 1103 4 0.49083476126004655 1103 5 0.4747754269065041 1103 6 0.5094946524885913 1103 7 0.4602606120032888 1103 8 0.6003584445157202 1103 9 0.6795910545285935 1103 10-11 0.8456010468189135 1103 12-13 0.7536502878998093 1103 14-15 1.0805422388141253 1103 16-17 0.9655848404552714 1103 18-19 1.0137586218335741 1103 20-21 1.386491174145938 1103 22-23 1.3485918924387263 1103 24-25 1.4592362655200475 1103 26-27 1.3732631472603032 1103 28-29 1.3840238622783119 1103 30-31 1.2581593908460107 1103 32-33 1.2555886761132982 1103 34-35 1.6295756164102357 1103 36-37 1.580424203529816 1103 38-39 1.6875150459735693 1103 40-41 1.3662435478874286 1103 42-43 1.239879160288183 1103 44-45 0.9002501795411177 1103 46-47 0.977622746225947 1103 48-49 0.8870427697791072 1103 50-51 0.9026703786776409 1103 52-53 0.9537283828418452 1103 54-55 0.7874735893644385 1103 56-57 0.7166201620383745 1103 58-59 0.7180210262468592 1103 60-61 0.6644522260630445 1103 62-63 0.625073130800569 1103 64-65 0.7579761750987295 1103 66-67 0.7530186991062244 1103 68-69 0.8704834778568156 1103 70-71 0.8528567360135284 1103 72-73 0.8402843595174403 1103 74-75 0.8198419241485979 1103 76-77 0.8350132825679708 1103 78-79 0.8919139337687163 1103 80-81 0.8051140868769018 1103 82-83 0.7910673759463123 1103 84-85 0.7304148803849309 1103 86-87 0.6880884544100212 1103 88-89 0.6755113690950587 1103 90-91 0.6742065368304608 1103 92-93 0.6926131700206071 1103 94-95 0.6863216122894613 1103 96-97 0.7383195187758318 1103 98-99 0.7066262966670607 1103 100-101 0.7168291385352568 1103 102-103 0.6499119403143219 1103 104-105 0.6317745719244918 1103 106-107 0.7134019605014217 1103 108-109 0.7833259439421951 1103 110-111 0.7963702142744431 1103 112-113 0.8137568348320698 1103 114-115 0.8224206971386145 1103 116-117 0.780496473101767 1103 118-119 0.7705730039794076 1103 120-121 0.795391873165439 1103 122-123 0.8743327968797239 1103 124-125 0.9693619985794406 1104 1 0.555013143770374 1104 2 0.6891442269081374 1104 3 0.589051133594225 1104 4 0.7573719536317256 1104 5 0.6470272561222288 1104 6 0.5712553586052991 1104 7 0.6216729554721283 1104 8 0.6723300354977653 1104 9 0.9867077996710165 1104 10-11 0.8929316299851777 1104 12-13 0.8629376228851982 1104 14-15 0.9542681526882788 1104 16-17 1.0018552947464343 1104 18-19 1.291313333221943 1104 20-21 1.5405953307623932 1104 22-23 1.3524646938489084 1104 24-25 1.7192408144846922 1104 26-27 1.7128573722763036 1104 28-29 1.580179987147389 1104 30-31 1.5543857495282083 1104 32-33 1.4610345021630948 1104 34-35 1.7959071699000049 1104 36-37 1.8082710391562813 1104 38-39 1.8383278982661153 1104 40-41 1.456196554147759 1104 42-43 1.4824945398240885 1104 44-45 1.3801601357206508 1104 46-47 1.2267355513780132 1104 48-49 1.0602246604975676 1104 50-51 0.9170352203168051 1104 52-53 0.9887021254107964 1104 54-55 0.8707879870209823 1104 56-57 0.9234446728871006 1104 58-59 0.9268088005375077 1104 60-61 0.8911408916205978 1104 62-63 0.9358842588887804 1104 64-65 0.9857410009632623 1104 66-67 0.9342360974286095 1104 68-69 0.9514250951971768 1104 70-71 0.9276659454658329 1104 72-73 0.8576718414270523 1104 74-75 0.8917923110857302 1104 76-77 0.9064488110824591 1104 78-79 0.8929811612297875 1104 80-81 0.9580102549244636 1104 82-83 0.9065134404498956 1104 84-85 0.9407577402529768 1104 86-87 0.9348762984025427 1104 88-89 0.9013101434425685 1104 90-91 0.9550135561764002 1104 92-93 0.9387925899909284 1104 94-95 0.9167948330114619 1104 96-97 0.7725081980903319 1104 98-99 0.7638551334344328 1104 100-101 0.8821874153581 1104 102-103 0.859576839365829 1104 104-105 0.9115463899879188 1104 106-107 0.8573074484237395 1104 108-109 0.984450913906187 1104 110-111 0.9834482198469239 1104 112-113 1.0301967930843148 1104 114-115 0.9937842676356432 1104 116-117 1.0287261371203762 1104 118-119 1.107298446448766 1104 120-121 1.0337416628675378 1104 122-123 1.0748192242732948 1104 124-125 1.3711794750412984 1105 1 0.0650805517179478 1105 2 0.30966553313351497 1105 3 0.3481392899201232 1105 4 0.34004197475103126 1105 5 0.30842128484918163 1105 6 0.302791985866925 1105 7 0.3543917193394037 1105 8 0.16725723589222952 1105 9 0.15125158995292054 1105 10-11 0.37389933644605833 1105 12-13 0.2995630930087998 1105 14-15 0.49418532176026275 1105 16-17 0.3694714105970647 1105 18-19 0.6312881624216473 1105 20-21 0.8661466995765252 1105 22-23 1.098521617526572 1105 24-25 1.1809714633510424 1105 26-27 0.9748457202820902 1105 28-29 0.7495700303455948 1105 30-31 0.7049027824167737 1105 32-33 0.8503013702224322 1105 34-35 1.4622276130952372 1105 36-37 1.3145640858549612 1105 38-39 1.4445209747161556 1105 40-41 0.9683841202117165 1105 42-43 0.6163266772287912 1105 44-45 0.7803481669186354 1105 46-47 0.7225522275640941 1105 48-49 0.6567452076510563 1105 50-51 0.665380725289701 1105 52-53 0.6360432174483037 1105 54-55 0.43174052153077014 1105 56-57 0.41747831096568433 1105 58-59 0.42499294253196496 1105 60-61 0.39436612617067013 1105 62-63 0.3628785210438359 1105 64-65 0.4676423505160088 1105 66-67 0.360670414541584 1105 68-69 0.14603654591547865 1105 70-71 0.336743681154843 1105 72-73 0.437696750251952 1105 74-75 0.5073597647512962 1105 76-77 0.4962610185082994 1105 78-79 0.46539542941684786 1105 80-81 0.44661149750514184 1105 82-83 0.4489982785326845 1105 84-85 0.41421988812867383 1105 86-87 0.42215153683116924 1105 88-89 0.5610718246444897 1105 90-91 0.43482397355866453 1105 92-93 0.40235715784062265 1105 94-95 0.45129695607028 1105 96-97 0.579632279634879 1105 98-99 0.45831315705848397 1105 100-101 0.453282392968692 1105 102-103 0.40557424573643885 1105 104-105 0.5388921005275833 1105 106-107 0.5117525250632156 1105 108-109 0.3701450104183621 1105 110-111 0.46124907067587273 1105 112-113 0.49233614910792767 1105 114-115 0.5169361789942606 1105 116-117 0.521356984526129 1105 118-119 0.48300356344120843 1105 120-121 0.3755270229965042 1105 122-123 0.5124114492814087 1105 124-125 0.643709030645649 1106 1 -0.04362494626542812 1106 2 0.22209874986149458 1106 3 -0.014355881960909755 1106 4 0.037676858469836816 1106 5 -0.1339839356372039 1106 6 -0.01827838185540287 1106 7 -0.21988689348609114 1106 8 -0.09278958887193056 1106 9 0.09464571877764172 1106 10-11 0.18862204033877106 1106 12-13 0.16113156281240038 1106 14-15 0.45807201916253604 1106 16-17 0.28379078963933324 1106 18-19 0.36793915539337263 1106 20-21 0.6926056133114002 1106 22-23 0.6666216077561913 1106 24-25 0.8500620055339709 1106 26-27 1.0283093192327328 1106 28-29 0.8531569232467362 1106 30-31 0.6575067479118175 1106 32-33 0.6420695072853135 1106 34-35 0.9690869662231734 1106 36-37 0.8122163416708545 1106 38-39 0.9502678059634704 1106 40-41 0.6236354243745339 1106 42-43 0.5369413298735992 1106 44-45 0.4259882042834775 1106 46-47 0.2659465161847123 1106 48-49 0.4247944969245552 1106 50-51 0.2990648334505295 1106 52-53 0.17782961689970023 1106 54-55 0.064561503554188 1106 56-57 0.024517137716792092 1106 58-59 0.022578586285725066 1106 60-61 0.010425089031361523 1106 62-63 0.13772815310236197 1106 64-65 0.13778024068052375 1106 66-67 0.02226105031924419 1106 68-69 0.030598161844665128 1106 70-71 0.07873813486745362 1106 72-73 0.10585693787973227 1106 74-75 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-0.9771194447083573 2216 84-85 -1.114168136119556 2216 86-87 -1.1597637215153966 2216 88-89 -1.1956256148412585 2216 90-91 -1.1612423315854699 2216 92-93 -1.1070909393872839 2216 94-95 -1.2303196713357245 2216 96-97 -1.046630100845885 2216 98-99 -1.097601173587762 2216 100-101 -1.0688022232961423 2216 102-103 -0.9553564183102168 2216 104-105 -1.1811853992762238 2216 106-107 -1.0839680845709267 2216 108-109 -1.1133507143124355 2216 110-111 -1.1226813930120692 2216 112-113 -1.077404577067469 2216 114-115 -1.200095934079954 2216 116-117 -1.2066940907250086 2216 118-119 -1.2768075469538118 2216 120-121 -1.1790972517992024 2216 122-123 -1.1875416176756666 2216 124-125 -1.3589812345074073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 154647.0 3 26398.0 4 16444.0 5 13273.0 6 12305.0 7 13810.0 8 14783.0 9 16207.0 10 17521.0 11 18901.0 12 20589.0 13 22661.0 14 25919.0 15 23148.0 16 17587.0 17 15704.0 18 14553.0 19 13949.0 20 13418.0 21 14365.0 22 17091.0 23 22070.0 24 29696.0 25 38319.0 26 46820.0 27 53925.0 28 62617.0 29 74646.0 30 85064.0 31 98484.0 32 122677.0 33 158047.0 34 208637.0 35 290124.0 36 453958.0 37 638709.0 38 230557.0 39 582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.551830334147354 19.476523215065786 13.783682709152409 23.187963741634448 2 14.938670238115856 20.593689285203435 41.03035795952742 23.43728251715328 3 18.482450463025028 26.563499246626176 29.227536855663327 25.726513434685472 4 12.14160999521484 17.258301229132268 38.81144538822029 31.788643387432604 5 13.429773934865066 38.85076339721866 33.94787434881425 13.771588319102026 6 30.464351148538938 37.45952365146453 18.595115931445886 13.48100926855065 7 27.77069348719215 33.036423872918945 21.805318135605724 17.387564504283183 8 25.991235342127922 35.17975886768189 20.42287149177171 18.40613429841848 9 26.89019736124741 16.353723694253624 20.24691001686903 36.50916892762993 10-11 24.516572835974543 27.154420572091958 27.929241342374862 20.39976524955864 12-13 25.461104495638548 24.55592504564462 28.145085277592745 21.837885181124083 14-15 22.614196423573986 25.912000501657413 26.118613430465842 25.35518964430277 16-17 22.27014386383339 29.08671847871154 26.397453612554866 22.24568404490021 18-19 22.11835553420315 27.928901626210774 28.509361311539344 21.443381528046732 20-21 23.30911082299607 26.95817066726962 28.24527516723635 21.487443342497958 22-23 22.80840922779113 26.859903288685004 28.07394201554385 22.257745467980012 24-25 22.36381644001079 27.11257272194566 28.277454889862273 22.246155948181272 26-27 22.27973648999633 27.290577081262818 28.095726935457304 22.333959493283547 28-29 22.218473613007017 27.375273535782817 28.14626481647146 22.259988034738704 30-31 22.54676306521205 27.16099784296282 28.079518537499286 22.212720554325845 32-33 22.417598629877773 27.064323903052305 27.871611978306397 22.646465488763525 34-35 22.591000861138596 27.109941901208 28.04220060199521 22.256856635658192 36-37 22.69980158116065 26.921457859690474 27.963120684294022 22.41561987485485 38-39 22.466146388707607 27.18701945510318 27.929674283762612 22.417159872426602 40-41 22.701678414768125 27.130079149325187 27.809111394455538 22.359131041451153 42-43 22.528218564926796 27.07234283164982 27.737237280337634 22.66220132308575 44-45 22.595047588744972 27.18899566803539 27.59783164419002 22.61812509902962 46-47 22.713042005789436 27.361410528354824 27.200911839091447 22.7246356267643 48-49 22.620897379968692 27.322567297082294 27.252206882393697 22.804328440555313 50-51 22.7132867895398 27.45114200560796 27.125411352610445 22.71015985224179 52-53 22.918987045431585 27.347977442150214 27.063550985262353 22.66948452715585 54-55 22.734318205400307 27.199006713695518 27.209190400135487 22.857484680768696 56-57 22.66627818073127 27.411317448385038 27.02317894812462 22.899225422759077 58-59 22.798360653371898 27.22430471361062 27.041747088498518 22.935587544518967 60-61 22.958078209366498 27.245042801815984 27.19446700097367 22.602411987843848 62-63 22.724657708473973 27.287218865613156 27.20559849352755 22.782524932385325 64-65 22.57970776026354 27.149143043449463 27.193165195973958 23.077984000313045 66-67 22.658824638664647 27.443559590989032 26.942430077263612 22.955185693082704 68-69 22.740742997908676 27.171722841636626 27.203237063932974 22.884297096521728 70-71 22.585965234673093 27.173626675300035 27.26884383311758 22.971564256909296 72-73 22.70171632592777 27.305067127321408 27.057671540382717 22.93554500636811 74-75 22.601159962221857 27.439632737424258 26.966459870342074 22.992747430011818 76-77 22.658913302873003 27.1958113490683 27.08482832749821 23.06044702056048 78-79 22.7058149681689 27.21216595465876 27.051928097731466 23.030090979440875 80-81 22.579577000441294 27.398951673320266 27.04960565880892 22.971865667429523 82-83 22.776150573270222 27.477428216549292 26.9590460793743 22.787375130806183 84-85 22.756072804706555 27.336945454195604 26.906281017444332 23.000700723653512 86-87 22.567041772181128 27.319233522425513 27.00875160677037 23.104973098622988 88-89 22.72892018785366 27.238732725501052 26.978400421330022 23.053946665315266 90-91 22.711963026704986 27.371025450173796 27.05183776695001 22.865173756171213 92-93 22.768397267740372 27.219183224540373 27.05326373277645 22.959155774942804 94-95 22.63563275612535 27.1876732137175 27.096864318487423 23.079829711669728 96-97 22.64966640654043 27.202533135624563 27.022609813527016 23.125190644308 98-99 22.403278577068306 27.125180634100253 27.00281766747419 23.46872312135725 100-101 22.42172018901428 27.15111825323276 27.160435226111385 23.26672633164158 102-103 22.579432718503114 27.007845860038067 27.153442445252956 23.259278976205863 104-105 22.45109196136777 27.00231401491931 27.198390339729333 23.348203683983588 106-107 22.393900463215363 27.095709581541172 27.20612570569469 23.30426424954878 108-109 22.429747242403884 27.024009005180865 26.973787804201454 23.572455948213797 110-111 22.371833540665083 27.17391269489265 27.085431905871065 23.368821858571202 112-113 22.39448657160129 27.25367639395101 27.1053699163461 23.2464671181016 114-115 22.303777333433818 27.33750667451306 27.016584862124205 23.342131129928916 116-117 22.18354790656804 27.300947179547208 27.076523192028745 23.438981721856003 118-119 22.17846485397849 27.10468041709894 27.119320249951496 23.597534478971074 120-121 22.164450380908246 27.222713067293526 27.182080716309542 23.430755835488686 122-123 22.238980439066708 27.280791352717348 27.033293192718244 23.446935015497697 124-125 22.223122149891203 27.148328270304628 26.93732819957566 23.691221380228512 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 463.0 1 427.0 2 480.5 3 568.0 4 489.0 5 394.0 6 388.0 7 470.0 8 689.0 9 833.5 10 827.5 11 834.0 12 904.5 13 1052.0 14 1228.0 15 1383.5 16 1559.5 17 1753.0 18 2000.5 19 2297.0 20 2644.0 21 3300.0 22 4263.0 23 5545.5 24 7232.0 25 9806.0 26 14057.5 27 19288.0 28 24064.5 29 29768.5 30 37073.0 31 44499.0 32 52207.5 33 59970.5 34 68169.5 35 75035.5 36 82051.5 37 89931.5 38 93125.0 39 94279.5 40 96973.5 41 100217.5 42 102771.5 43 106299.5 44 110582.5 45 113446.0 46 114232.0 47 114317.5 48 117350.0 49 118156.0 50 114845.0 51 110488.5 52 106434.5 53 104606.0 54 101479.5 55 95525.5 56 87946.5 57 79708.0 58 73174.5 59 64921.5 60 54400.0 61 45016.5 62 36389.5 63 27495.0 64 19740.0 65 14680.0 66 11210.0 67 8697.0 68 6857.5 69 5405.5 70 4249.0 71 3406.0 72 2770.5 73 2409.5 74 1976.5 75 1481.0 76 1050.5 77 666.5 78 427.0 79 297.5 80 230.0 81 168.0 82 124.0 83 94.0 84 75.5 85 59.0 86 41.0 87 36.5 88 29.5 89 21.0 90 17.0 91 15.0 92 13.5 93 12.5 94 13.0 95 10.0 96 5.0 97 5.0 98 5.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011513033941001314 2 0.004585971736944813 3 0.007408108190449313 4 0.0074722476553016875 5 0.013822054675686812 6 8.338130430808751E-4 7 0.0062535978231065634 8 0.0 9 0.0020845326077021877 10-11 0.0 12-13 0.0030145548480616253 14-15 0.017301620643928158 16-17 0.02717909823119391 18-19 0.0050990874557638125 20-21 0.007616561451219532 22-23 0.0018921142131450624 24-25 0.010775430095199 26-27 0.016820574657535343 28-29 3.6880192290115625E-4 30-31 0.0012507195646213126 32-33 0.007536387120154062 34-35 0.007696735782285 36-37 0.009797303256200282 38-39 0.0 40-41 0.021807418049807504 42-43 0.00854658369157897 44-45 0.014463449324210564 46-47 0.003559740299306813 48-49 0.02196776671193844 50-51 0.004425623074813876 52-53 0.0 54-55 0.015185018303799782 56-57 0.0037521586938639375 58-59 3.6880192290115625E-4 60-61 0.0036238797641591877 62-63 0.02320245141034666 64-65 0.014639832852554593 66-67 0.0015714168888831877 68-69 0.018119398820795937 70-71 0.0022288464036200315 72-73 0.01039059330608475 74-75 0.0 76-77 0.005147192054403094 78-79 8.979525079332501E-4 80-81 0.002982485115635438 82-83 0.001346928761899875 84-85 0.0 86-87 0.004778390131501937 88-89 0.014720007183620064 90-91 0.019354083519204156 92-93 0.004056821151912719 94-95 0.02145465099311944 96-97 0.0156500294239795 98-99 7.376038458023125E-4 100-101 0.007680700916071907 102-103 0.0 104-105 0.0010101965714249062 106-107 3.2069732426187504E-5 108-109 0.0 110-111 4.4897625396662504E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.02150275559175872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3118205.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.09212195739832 #Duplication Level Percentage of deduplicated Percentage of total 1 76.18872362354426 42.7358717727036 2 12.46188774495181 13.98027454418489 3 4.683878179629997 7.88185998256208 4 2.2139103058820475 4.967317075211073 5 1.2579769823614249 3.5281299157108483 6 0.7644766964166034 2.5728672053393455 7 0.5105852198114095 2.0047865893514625 8 0.35180086685935863 1.5786605702874874 9 0.24078755996501544 1.2155656661443808 >10 1.2064790787038109 12.096920464569278 >50 0.07923368251201798 3.0511774362491586 >100 0.039160748136929095 3.784643470572449 >500 8.67877283436992E-4 0.28523866547647614 >1k 2.3143394184520222E-4 0.31668664163747 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4173 0.13382699341448046 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.2069732426187504E-5 0.0 3 0.0 0.0 0.0 9.62091972785625E-5 0.0 4 0.0 0.0 0.0 1.2827892970475001E-4 0.0 5 0.0 0.0 0.0 1.2827892970475001E-4 0.0 6 0.0 0.0 0.0 1.2827892970475001E-4 0.0 7 3.2069732426187504E-5 0.0 0.0 2.886275918356875E-4 0.0 8 3.2069732426187504E-5 0.0 0.0 3.8483678911425E-4 0.0 9 3.2069732426187504E-5 0.0 0.0 4.169065215404375E-4 0.0 10-11 3.2069732426187504E-5 0.0 0.0 4.810459863928125E-4 3.2069732426187504E-5 12-13 4.810459863928125E-5 0.0 0.0 6.894992471630312E-4 3.2069732426187504E-5 14-15 6.413946485237501E-5 0.0 0.0 7.536387120154063E-4 6.413946485237501E-5 16-17 6.413946485237501E-5 0.0 0.0 8.498479092939688E-4 8.017433106546876E-5 18-19 8.017433106546876E-5 0.0 0.0 8.819176417201563E-4 1.2827892970475001E-4 20-21 9.62091972785625E-5 0.0 0.0 9.460571065725313E-4 1.2827892970475001E-4 22-23 9.62091972785625E-5 0.0 0.0 0.0010583011700641875 1.2827892970475001E-4 24-25 9.62091972785625E-5 0.0 0.0 0.0012827892970475002 1.2827892970475001E-4 26-27 9.62091972785625E-5 0.0 0.0 0.0015553820226700938 1.2827892970475001E-4 28-29 1.92418394557125E-4 0.0 0.0 0.002068497741489094 1.2827892970475001E-4 30-31 2.2448812698331252E-4 0.0 0.0 0.003415426503388969 1.4431379591784376E-4 32-33 2.2448812698331252E-4 0.0 0.0 0.006638434612220813 1.603486621309375E-4 34-35 2.5655785940950003E-4 0.0 0.0 0.010695255764133531 1.92418394557125E-4 36-37 2.886275918356875E-4 0.0 0.0 0.018311817215353063 1.92418394557125E-4 38-39 3.20697324261875E-4 0.0 0.0 0.03282337113820291 2.0845326077021878E-4 40-41 3.527670566880625E-4 0.0 0.0 0.05091070022657266 2.2448812698331252E-4 42-43 3.8483678911425E-4 0.0 0.0 0.06909423851222096 2.2448812698331252E-4 44-45 4.169065215404375E-4 0.0 0.0 0.09102993549173322 2.4052299319640626E-4 46-47 4.169065215404375E-4 0.0 0.0 0.11716676741907604 2.886275918356875E-4 48-49 4.169065215404375E-4 0.0 0.0 0.1478575013509375 3.5276705668806253E-4 50-51 4.169065215404375E-4 0.0 0.0 0.1831662767521699 3.5276705668806253E-4 52-53 4.3294138775353127E-4 0.0 0.0 0.21876367974523805 3.688019229011563E-4 54-55 4.4897625396662504E-4 0.0 0.0 0.25623716208523817 3.8483678911425E-4 56-57 4.810459863928125E-4 0.0 0.0 0.30289862276534096 3.8483678911425E-4 58-59 4.810459863928125E-4 0.0 0.0 0.3569681916358931 3.8483678911425E-4 60-61 4.810459863928125E-4 0.0 0.0 0.41544734871504596 3.8483678911425E-4 62-63 4.810459863928125E-4 3.2069732426187504E-5 0.0 0.47702123497332605 4.169065215404375E-4 64-65 5.131157188190001E-4 3.2069732426187504E-5 0.0 0.5389478882882941 4.169065215404375E-4 66-67 5.131157188190001E-4 3.2069732426187504E-5 0.0 0.6115537625011827 4.169065215404375E-4 68-69 5.131157188190001E-4 3.2069732426187504E-5 0.0 0.7007557232446231 4.169065215404375E-4 70-71 5.131157188190001E-4 3.2069732426187504E-5 0.0 0.8011179508723769 4.169065215404375E-4 72-73 5.131157188190001E-4 3.2069732426187504E-5 0.0 0.9098824483957918 4.970808526059062E-4 74-75 5.131157188190001E-4 3.2069732426187504E-5 0.0 1.017332086889733 5.131157188190001E-4 76-77 5.131157188190001E-4 3.2069732426187504E-5 0.0 1.1412816027169477 5.131157188190001E-4 78-79 5.131157188190001E-4 3.2069732426187504E-5 0.0 1.2792776613468324 5.131157188190001E-4 80-81 5.131157188190001E-4 3.2069732426187504E-5 0.0 1.4373333376092976 5.131157188190001E-4 82-83 5.451854512451875E-4 3.2069732426187504E-5 0.0 1.6104617881120709 5.131157188190001E-4 84-85 5.612203174582813E-4 3.2069732426187504E-5 0.0 1.7838628313404667 5.131157188190001E-4 86-87 5.77255183671375E-4 3.2069732426187504E-5 0.0 1.970685057589222 5.451854512451875E-4 88-89 5.77255183671375E-4 3.2069732426187504E-5 0.0 2.1836761854977462 5.77255183671375E-4 90-91 6.093249160975626E-4 3.2069732426187504E-5 0.0 2.426315781034281 5.77255183671375E-4 92-93 6.4139464852375E-4 3.2069732426187504E-5 0.0 2.689512075056002 5.77255183671375E-4 94-95 6.4139464852375E-4 3.2069732426187504E-5 0.0 2.956300179109456 6.4139464852375E-4 96-97 6.4139464852375E-4 3.2069732426187504E-5 0.0 3.2314264135937183 6.4139464852375E-4 98-99 7.376038458023125E-4 3.2069732426187504E-5 0.0 3.5326734451391104 6.4139464852375E-4 100-101 7.696735782285E-4 3.2069732426187504E-5 0.0 3.880533832765966 6.4139464852375E-4 102-103 8.017433106546876E-4 3.2069732426187504E-5 0.0 4.257128700646685 6.4139464852375E-4 104-105 0.0010422663038510938 3.2069732426187504E-5 0.0 4.646246799039832 6.734643809499375E-4 106-107 0.0010743360362772813 3.2069732426187504E-5 0.0 5.0393736139862515 6.894992471630312E-4 108-109 0.001106405768703469 3.2069732426187504E-5 0.0 5.450491548823763 7.055341133761251E-4 110-111 0.00115451036734275 3.2069732426187504E-5 0.0 5.912343800359501 7.376038458023125E-4 112-113 0.0011865800997689375 4.810459863928125E-5 0.0 6.408478595858836 7.376038458023125E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4375 0.0 58.07423 1 GTATCAA 8625 0.0 56.84624 1 ATCAACG 10845 0.0 44.824886 3 TCAACGC 10890 0.0 44.421104 4 CAACGCA 11130 0.0 43.57016 5 AACGCAG 11320 0.0 42.77943 6 TATCAAC 11635 0.0 41.883614 2 ACGCAGA 13475 0.0 35.629417 7 GTACATG 15315 0.0 35.08362 1 CGCAGAG 13485 0.0 34.634075 8 TACATGG 15665 0.0 33.31163 2 ACATGGG 16290 0.0 31.303034 3 GCAGAGT 15025 0.0 29.580936 9 CATGGGG 8400 0.0 26.988104 4 AGAGTAC 14120 0.0 24.65393 10-11 GAGTACT 6200 0.0 23.75712 12-13 CATGGGA 8810 0.0 22.625364 4 CAGAGTA 15050 0.0 22.478067 10-11 AGTACTT 6800 0.0 21.092031 12-13 GTACTTT 7095 0.0 20.424753 14-15 >>END_MODULE