FastQCFastQC Report
Thu 26 May 2016
SRR1364577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118205
Sequences flagged as poor quality0
Sequence length125
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62230.19956994488816482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.1835030089426449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.10012170463455738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.066.619461
GTATCAA104550.053.4900781
ATCAACG136550.041.3542753
TCAACGC138950.040.7257234
CAACGCA141400.040.058035
TATCAAC143800.038.6479532
AACGCAG146000.038.5921676
ACGCAGA172250.032.2618837
CGCAGAG174450.031.6844948
GTACATG144950.031.3011861
TACATGG149150.029.5936622
GCAGAGT187100.028.4283629
ACATGGG154750.027.8298823
GAGTACT107600.025.68393312-13
AGAGTAC179900.024.7041910-11
TGGTATC27000.024.2679272
GTGGTAT28650.024.1394711
GTACTTT116650.023.6913114-15
CAGAGTA188400.023.68435310-11
CATGGGG82150.023.4931474
Produced by FastQC (version 0.11.5)