##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364577_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3118205 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.647661074239828 31.0 30.0 33.0 26.0 34.0 2 29.56770738293345 31.0 30.0 34.0 25.0 34.0 3 29.840802961960488 31.0 30.0 34.0 26.0 34.0 4 33.3751049081122 35.0 33.0 37.0 28.0 37.0 5 32.78144381142356 35.0 33.0 37.0 26.0 37.0 6 32.79787730441071 35.0 33.0 37.0 25.0 37.0 7 32.57410882222304 35.0 33.0 37.0 25.0 37.0 8 32.48607804810781 35.0 33.0 37.0 23.0 37.0 9 33.70037858319129 38.0 33.0 39.0 22.0 39.0 10-11 33.61113268691443 37.5 33.0 39.0 22.0 39.0 12-13 33.49984221691646 37.0 33.0 39.0 20.5 39.0 14-15 34.40517509272161 38.0 33.0 41.0 19.0 41.0 16-17 34.399567539658236 38.0 33.0 41.0 19.0 41.0 18-19 34.4025278966585 38.5 33.0 41.0 19.0 41.0 20-21 34.41902889643241 39.0 33.0 41.0 19.0 41.0 22-23 34.367501495251275 39.0 33.0 41.0 19.0 41.0 24-25 34.32411114727864 39.0 33.0 41.0 19.0 41.0 26-27 34.18842170415351 39.0 33.0 41.0 19.0 41.0 28-29 34.02893379364089 38.0 33.0 40.5 18.0 41.0 30-31 33.86912518580401 38.0 33.0 40.0 17.5 41.0 32-33 33.662805363983445 38.0 32.5 40.0 16.5 41.0 34-35 33.47418290330495 38.0 32.0 40.0 16.0 41.0 36-37 33.26393967041936 38.0 32.0 40.0 15.0 41.0 38-39 33.0807860612115 38.0 32.0 40.0 10.5 41.0 40-41 33.11423912154589 38.0 32.0 40.0 9.0 41.0 42-43 32.974206795255604 38.0 32.0 40.0 9.0 41.0 44-45 32.79551681175548 38.0 31.5 40.0 7.5 41.0 46-47 32.560321242509715 38.0 31.0 40.0 2.0 41.0 48-49 32.2881994609078 38.0 31.0 40.0 2.0 41.0 50-51 32.1146371069253 38.0 31.0 40.0 2.0 41.0 52-53 31.915854955014183 37.0 30.0 40.0 2.0 41.0 54-55 31.71737105161463 37.0 30.0 40.0 2.0 41.0 56-57 31.497891896138967 37.0 30.0 40.0 2.0 41.0 58-59 31.290184737693643 36.5 30.0 40.0 2.0 41.0 60-61 31.05337926788008 36.0 29.0 40.0 2.0 41.0 62-63 30.879851388859937 36.0 29.0 39.5 2.0 41.0 64-65 30.751812180405075 35.5 29.0 39.0 2.0 41.0 66-67 30.4546181216437 35.0 29.0 39.0 2.0 41.0 68-69 30.168044596169914 35.0 29.0 39.0 2.0 40.5 70-71 29.858884678845683 35.0 29.0 38.0 2.0 40.0 72-73 29.573695443372067 35.0 29.0 37.0 2.0 40.0 74-75 29.222527223194113 35.0 28.0 37.0 2.0 39.0 76-77 28.911169406758056 34.0 28.0 36.0 2.0 39.0 78-79 28.62472432056263 34.0 28.0 36.0 2.0 39.0 80-81 28.34252703077572 34.0 27.5 35.5 2.0 37.0 82-83 28.0534515851267 34.0 27.0 35.0 2.0 37.0 84-85 27.83295325355453 34.0 27.0 35.0 2.0 36.5 86-87 27.621895449465317 34.0 26.0 35.0 2.0 36.0 88-89 27.443526965032767 34.0 26.0 35.0 2.0 36.0 90-91 27.299393721708483 34.0 26.0 35.0 2.0 35.5 92-93 27.188695900365758 34.0 26.0 35.0 2.0 35.0 94-95 27.07629613832317 34.0 26.0 35.0 2.0 35.0 96-97 26.960618849626627 34.0 25.5 35.0 2.0 35.0 98-99 26.88069113480352 34.0 25.0 35.0 2.0 35.0 100-101 26.780884354941385 34.0 25.0 35.0 2.0 35.0 102-103 25.535516587267352 31.5 23.5 34.0 2.0 35.0 104-105 26.44956200762939 33.0 25.0 35.0 2.0 35.0 106-107 26.610926157837604 34.0 25.0 35.0 2.0 35.0 108-109 26.61822635137844 34.0 25.0 35.0 2.0 35.0 110-111 26.539299372555682 34.0 25.0 35.0 2.0 35.0 112-113 26.449838288374238 34.0 25.0 35.0 2.0 35.0 114-115 26.34955831961016 34.0 25.0 35.0 2.0 35.0 116-117 26.223251357752297 34.0 24.5 35.0 2.0 35.0 118-119 26.08487142442527 34.0 24.0 35.0 2.0 35.0 120-121 25.886632052735468 33.5 23.5 35.0 2.0 35.0 122-123 25.584106721655566 33.0 20.5 35.0 2.0 35.0 124-125 23.805642669420386 31.0 9.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1339073080483466 1101 2 1.2927111687936765 1101 3 1.1331242172968317 1101 4 1.0807317351811747 1101 5 1.0603473346638737 1101 6 1.214492110791511 1101 7 1.3146416900408582 1101 8 1.3652012405358604 1101 9 1.6167185071744044 1101 10-11 1.5255440740542383 1101 12-13 1.519602492902493 1101 14-15 1.7372826447561138 1101 16-17 1.7830754035046894 1101 18-19 1.833595006115921 1101 20-21 1.7635846423248296 1101 22-23 1.8400617560727568 1101 24-25 1.822371197463724 1101 26-27 1.7666576612492975 1101 28-29 1.961558205255976 1101 30-31 1.899532877005882 1101 32-33 1.9892679190884692 1101 34-35 2.029073999979836 1101 36-37 2.139167206576907 1101 38-39 2.1222759037550247 1101 40-41 1.8824139853454653 1101 42-43 1.8968369816316297 1101 44-45 1.8461237237696508 1101 46-47 1.833153153009036 1101 48-49 1.99602250384509 1101 50-51 2.0595896157089086 1101 52-53 2.1182370962819235 1101 54-55 2.0710755351474113 1101 56-57 2.096881543547209 1101 58-59 2.0969667584781106 1101 60-61 2.0964085485006443 1101 62-63 2.0620212708123056 1101 64-65 1.9558786135190758 1101 66-67 2.0037128979113987 1101 68-69 2.052872853157236 1101 70-71 1.9990723574074813 1101 72-73 1.9366376974541097 1101 74-75 1.905584324368352 1101 76-77 1.9880470685070826 1101 78-79 1.9644750595783407 1101 80-81 1.796333836933485 1101 82-83 1.9365131448459252 1101 84-85 1.906859519706579 1101 86-87 1.8997945094727129 1101 88-89 1.9056638681579194 1101 90-91 1.8022274290598759 1101 92-93 1.7716171177434603 1101 94-95 1.8353143366583033 1101 96-97 1.9205916651185788 1101 98-99 1.8721777742405905 1101 100-101 1.7639365703982293 1101 102-103 1.6702971443305579 1101 104-105 1.7929417293679464 1101 106-107 1.7870071040418374 1101 108-109 1.8028434153179873 1101 110-111 1.7275549443960472 1101 112-113 1.8053408955636812 1101 114-115 1.8468844443044006 1101 116-117 1.7780414725164277 1101 118-119 1.8563205511876717 1101 120-121 1.9036390251358348 1101 122-123 1.8199406734139778 1101 124-125 2.063839900998847 1102 1 0.7220162924256002 1102 2 0.04340113603687712 1102 3 0.6518630517104569 1102 4 0.7830611932354756 1102 5 1.0563362113998451 1102 6 0.8427127560940733 1102 7 0.7774255476496279 1102 8 0.9342200410043873 1102 9 1.4789944498415224 1102 10-11 1.4492806369996174 1102 12-13 1.4068133673874001 1102 14-15 1.664019266726953 1102 16-17 1.7221523977907438 1102 18-19 1.7905110857595048 1102 20-21 1.636912091800042 1102 22-23 1.7687928042638603 1102 24-25 1.7178037825129593 1102 26-27 1.735696330251443 1102 28-29 1.377621496133024 1102 30-31 1.6807024713514025 1102 32-33 1.7909607197099362 1102 34-35 1.8354308833852215 1102 36-37 1.5958052318743867 1102 38-39 1.0642175021803055 1102 40-41 0.5664134265502554 1102 42-43 0.9971794617323368 1102 44-45 0.7474641314378516 1102 46-47 0.894916940712335 1102 48-49 0.6284500570898714 1102 50-51 0.6719127670709213 1102 52-53 0.28501747263391763 1102 54-55 0.4074547345408952 1102 56-57 0.41463221139936124 1102 58-59 0.44996295125747565 1102 60-61 0.35010513531784326 1102 62-63 0.4805254166896269 1102 64-65 0.44587325139003653 1102 66-67 0.3464321568941351 1102 68-69 0.5516167125227476 1102 70-71 0.017361842915637027 1102 72-73 0.2243578340318777 1102 74-75 0.2943101404733355 1102 76-77 -0.013247637250952238 1102 78-79 0.07939058151689338 1102 80-81 0.11211801443226221 1102 82-83 -0.15317064372328915 1102 84-85 -0.06189531310105423 1102 86-87 0.18879103288814747 1102 88-89 0.09021994367750352 1102 90-91 0.08559175076456782 1102 92-93 0.18707348974532678 1102 94-95 0.28866817472325224 1102 96-97 0.0812415495516916 1102 98-99 0.05303262967725075 1102 100-101 0.22862816543616304 1102 102-103 0.10267241175564479 1102 104-105 0.27465140675715816 1102 106-107 0.4132422587080349 1102 108-109 0.4585895525370951 1102 110-111 0.42820398705432794 1102 112-113 0.6481286412221543 1102 114-115 0.712469395270432 1102 116-117 0.5918795032025059 1102 118-119 0.7989521557911559 1102 120-121 1.028716077512918 1102 122-123 1.0573445565111506 1102 124-125 1.1901033224794766 1103 1 0.8369442969195475 1103 2 0.36166652653712106 1103 3 0.8084155168027998 1103 4 0.7678143304561047 1103 5 0.8635838048388393 1103 6 0.9148732105172144 1103 7 1.0647225588196818 1103 8 1.1325325708347478 1103 9 1.457538630196595 1103 10-11 1.4007790195313419 1103 12-13 1.3329381805487088 1103 14-15 1.4977189774093134 1103 16-17 1.3704754946876037 1103 18-19 1.442616583580289 1103 20-21 1.5847711412747358 1103 22-23 1.5327614055417271 1103 24-25 1.4263826102756667 1103 26-27 1.5375564482176145 1103 28-29 1.5687815361228914 1103 30-31 1.5855371596131178 1103 32-33 1.6794362337741475 1103 34-35 1.6067905780794902 1103 36-37 1.6112205784761287 1103 38-39 1.5962394455384725 1103 40-41 1.5403401720312644 1103 42-43 1.6540565007538817 1103 44-45 1.519842192793618 1103 46-47 1.5656438291826404 1103 48-49 1.5180909732427565 1103 50-51 1.5617065914258461 1103 52-53 1.4576420530447791 1103 54-55 1.5141372256187928 1103 56-57 1.477045411197448 1103 58-59 1.5325487595693161 1103 60-61 1.6066035080288863 1103 62-63 1.4824243067548188 1103 64-65 1.4950504933071187 1103 66-67 1.3932825818355532 1103 68-69 1.456192920124355 1103 70-71 1.3998368738017213 1103 72-73 1.4015697587110871 1103 74-75 1.4561040669634764 1103 76-77 1.5524813233857415 1103 78-79 1.3882183049425905 1103 80-81 1.496887100459574 1103 82-83 1.5393178415132631 1103 84-85 1.5595318890422156 1103 86-87 1.5825486070982215 1103 88-89 1.5372617739405605 1103 90-91 1.5309977677072304 1103 92-93 1.5016559825909503 1103 94-95 1.4200690770485913 1103 96-97 1.5534457649862858 1103 98-99 1.5207467257118523 1103 100-101 1.4484898115022915 1103 102-103 1.253306180407602 1103 104-105 1.2778522654786215 1103 106-107 1.436547452480145 1103 108-109 1.4810288753806198 1103 110-111 1.438430786156328 1103 112-113 1.389979900922544 1103 114-115 1.4291381570184392 1103 116-117 1.463259526732891 1103 118-119 1.3169976216750499 1103 120-121 1.3063312005956753 1103 122-123 1.3607610580264868 1103 124-125 1.6254240172346357 1104 1 0.7264408109085316 1104 2 0.9450508094973991 1104 3 0.6729069957482459 1104 4 0.47375241684608227 1104 5 0.8319333560871414 1104 6 0.9436858023448522 1104 7 0.9785871060601323 1104 8 0.9236403354405027 1104 9 1.125102457856869 1104 10-11 1.0769589164092537 1104 12-13 1.0689854237477334 1104 14-15 1.2659424416293206 1104 16-17 1.2299526755768184 1104 18-19 1.194602041567478 1104 20-21 1.1822170627060444 1104 22-23 1.225673146456181 1104 24-25 1.2949440735748823 1104 26-27 1.2589814480800428 1104 28-29 1.4637415033996959 1104 30-31 1.4674858773737043 1104 32-33 1.5514990791882255 1104 34-35 1.6335256687561355 1104 36-37 1.6974896754149356 1104 38-39 1.7462070708505237 1104 40-41 1.5980288394230797 1104 42-43 1.5388436073739058 1104 44-45 1.560896470379511 1104 46-47 1.6271685577580257 1104 48-49 1.6172354927491952 1104 50-51 1.4562947175275553 1104 52-53 1.56464602788607 1104 54-55 1.244387015137466 1104 56-57 1.3818154592000376 1104 58-59 1.5034265263211566 1104 60-61 1.5912319328214828 1104 62-63 1.5473758547784904 1104 64-65 1.5660885193499325 1104 66-67 1.6097942046236646 1104 68-69 1.2496461162305899 1104 70-71 0.8253711845510345 1104 72-73 0.899686469002571 1104 74-75 1.3736999758301067 1104 76-77 1.5060029740729455 1104 78-79 1.533479494386789 1104 80-81 1.4990302497738348 1104 82-83 1.5446519244474928 1104 84-85 1.4295330996537743 1104 86-87 1.4142976289791065 1104 88-89 1.4781253068352633 1104 90-91 1.4893460900013729 1104 92-93 1.4098291330608 1104 94-95 1.3896735372350655 1104 96-97 1.4626272264879248 1104 98-99 1.5364015656818424 1104 100-101 1.4966410792510487 1104 102-103 1.3089209051126964 1104 104-105 1.421593770562275 1104 106-107 1.4542033966746182 1104 108-109 1.435257651617416 1104 110-111 1.5023496825188083 1104 112-113 1.4477545217351562 1104 114-115 1.4295722346333122 1104 116-117 1.485038905615788 1104 118-119 1.484645366727591 1104 120-121 1.4813344380644686 1104 122-123 1.5434420857256441 1104 124-125 1.9490170206335193 1105 1 0.46905235678447355 1105 2 0.2985307804568045 1105 3 0.5379032299431579 1105 4 0.570968676513175 1105 5 0.3533512676295132 1105 6 0.7969619579062766 1105 7 0.894992070982795 1105 8 0.8872578274281793 1105 9 1.0492089156087374 1105 10-11 1.018550772316658 1105 12-13 1.0611882077361727 1105 14-15 1.1136468949994125 1105 16-17 1.1560664376989251 1105 18-19 1.2757470046672665 1105 20-21 1.2588236487091038 1105 22-23 1.2025664057854755 1105 24-25 1.1179438458241222 1105 26-27 1.128272509822537 1105 28-29 1.062476819003784 1105 30-31 1.211570414571547 1105 32-33 1.2766484872588322 1105 34-35 1.3570893777059894 1105 36-37 1.3914147907333572 1105 38-39 1.424660660011952 1105 40-41 1.1746695041196062 1105 42-43 1.2235253514178197 1105 44-45 1.2331780761237638 1105 46-47 1.1510444224318945 1105 48-49 0.9714400190564518 1105 50-51 0.9189496323720476 1105 52-53 1.1558831552433944 1105 54-55 1.1080119832753503 1105 56-57 1.0139856400221667 1105 58-59 0.7876793531560722 1105 60-61 0.7952517847185412 1105 62-63 0.953952692344334 1105 64-65 0.8030549333015635 1105 66-67 0.9794174241670035 1105 68-69 1.0043052099839933 1105 70-71 1.0561779658753565 1105 72-73 1.1222146454049806 1105 74-75 1.0816518850287054 1105 76-77 0.9992737273952343 1105 78-79 0.8624925870997373 1105 80-81 0.8885017359412792 1105 82-83 1.0130367118646753 1105 84-85 0.9342191206831743 1105 86-87 1.088713786891745 1105 88-89 1.0364308499792152 1105 90-91 0.9184088084434272 1105 92-93 0.9634974927890632 1105 94-95 0.8618092248734079 1105 96-97 0.9639463893605082 1105 98-99 0.8633542474524489 1105 100-101 0.9292181355918885 1105 102-103 0.5837727923243357 1105 104-105 0.7239340828763474 1105 106-107 0.7844099551520181 1105 108-109 0.7844495086048262 1105 110-111 0.7103214587929862 1105 112-113 0.7001888131614038 1105 114-115 0.7405563302457203 1105 116-117 0.48213955313285695 1105 118-119 0.6401541802293522 1105 120-121 0.717448136699506 1105 122-123 0.8342908911141969 1105 124-125 1.2751933175230121 1106 1 0.017583512875848584 1106 2 -0.28841051600841894 1106 3 0.22636788354673953 1106 4 0.09175994928294529 1106 5 0.33316630615858145 1106 6 0.49815230038998237 1106 7 0.6037857306467487 1106 8 0.6272304064518153 1106 9 0.839184142678711 1106 10-11 0.6280049140894022 1106 12-13 0.7189140655433874 1106 14-15 0.7081114965551905 1106 16-17 0.706929586664323 1106 18-19 0.7652183952659684 1106 20-21 0.8530219618122743 1106 22-23 0.7555780106202619 1106 24-25 0.7278087771986321 1106 26-27 0.812746032555097 1106 28-29 0.9267953762385659 1106 30-31 0.8955998188433014 1106 32-33 0.7887249482270846 1106 34-35 0.7443532564364261 1106 36-37 0.878250462108987 1106 38-39 0.7488496729937779 1106 40-41 0.7605976945817048 1106 42-43 0.6582532920504462 1106 44-45 0.7153677958600184 1106 46-47 0.6912973519472985 1106 48-49 0.881315245852484 1106 50-51 0.8657245858714475 1106 52-53 0.7708918024843641 1106 54-55 0.7725227749772863 1106 56-57 0.3573753858736346 1106 58-59 0.836275683146475 1106 60-61 0.8073821197329281 1106 62-63 0.6429523197916076 1106 64-65 0.781533963970503 1106 66-67 0.6733649033638578 1106 68-69 0.7981995456302577 1106 70-71 0.8276024869670451 1106 72-73 0.8561181761638643 1106 74-75 0.7055946068733547 1106 76-77 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-4.695119570732803 2216 106-107 -4.773863084237103 2216 108-109 -4.975019194442947 2216 110-111 -5.0905163869956915 2216 112-113 -4.974286911209887 2216 114-115 -5.096771463191022 2216 116-117 -5.138738570457342 2216 118-119 -5.169871778719472 2216 120-121 -5.379943626847396 2216 122-123 -5.45295511475452 2216 124-125 -5.516928156854092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 122780.0 3 39373.0 4 18470.0 5 14621.0 6 15263.0 7 17958.0 8 20069.0 9 21812.0 10 24025.0 11 25978.0 12 27921.0 13 31353.0 14 35168.0 15 30420.0 16 21468.0 17 18783.0 18 17092.0 19 16042.0 20 16056.0 21 16488.0 22 18543.0 23 23504.0 24 31120.0 25 40907.0 26 49133.0 27 54899.0 28 63751.0 29 75384.0 30 86466.0 31 102282.0 32 123986.0 33 153074.0 34 196674.0 35 268288.0 36 424581.0 37 630883.0 38 223115.0 39 475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87566803078856 20.664504658119426 15.069400731162485 23.390426579929528 2 15.610813147568281 21.885033820750664 38.57153027832688 23.932622753354174 3 18.787122719641587 26.350127717709388 29.908072112000333 24.954677450648692 4 13.033997489751961 18.473242746121475 37.29207795251072 31.200681811615837 5 13.132555428523782 39.0702663872324 34.045356222570355 13.751821961673464 6 30.43087289001204 38.18757907193401 17.757331541704282 13.62421649634966 7 27.151828545149353 33.28223485502036 21.796269725562688 17.7696668742676 8 25.891331754787196 35.542239579174094 19.950135645284455 18.616293020754256 9 26.32434556023251 16.100412908398475 20.115678492683905 37.459563038685104 10-11 24.540463312910195 27.31307695638021 27.562083192359683 20.58437653834991 12-13 25.510975064179554 24.67525387201932 28.13463194369838 21.679139120102754 14-15 22.700410669156135 25.890228139375765 26.22168501831263 25.18767617315547 16-17 22.034394843178756 28.976492848438202 26.6705631454044 22.31854916297864 18-19 21.930328119893822 27.412617509241834 29.250496847659146 21.4065575232052 20-21 22.99500140386582 26.4590463237236 29.121308277869545 21.424643994541036 22-23 22.515790334503343 26.40195240531011 28.94219591078842 22.14006134939813 24-25 22.181912350214304 26.720356743703512 29.062313093590703 22.03541781249148 26-27 22.005143985081162 26.92996451484107 28.971299192965184 22.093592307112587 28-29 21.960903789199236 26.90124606945342 29.101165574425032 22.036684566922315 30-31 22.282659414631173 26.674144259277373 29.070090003704053 21.973106322387398 32-33 22.19969501684463 26.562621764765304 28.84755813039874 22.39012508799133 34-35 22.357462065515257 26.797131683131802 28.816562734008826 22.028843517344114 36-37 22.506313728571406 26.50112805283809 28.795027908684645 22.197530309905858 38-39 22.278378105352278 26.780711980129595 28.741006444412733 22.199903470105397 40-41 22.400355332635282 26.692199518633313 28.685509772449215 22.221935376282186 42-43 22.40247193497541 26.636141626352337 28.501269159660765 22.46011727901148 44-45 22.478332887029556 26.741025686252186 28.31791046451404 22.462730962204216 46-47 22.605617655029096 26.96583451055976 27.83522892176749 22.593318912643653 48-49 22.579977903954358 27.09876355146631 27.629421413922433 22.6918371306569 50-51 22.731478964897907 27.188482987565436 27.422768455372314 22.657269592164337 52-53 22.888697183155053 27.134681651783637 27.322850806794293 22.653770358267018 54-55 22.682520873387094 26.9773956490994 27.481467703374218 22.858615774139288 56-57 22.63395345585259 27.19208809789396 27.24415696542576 22.929801480827688 58-59 22.752287987813432 27.00995369906396 27.333442905534067 22.904315407588545 60-61 22.885426070447583 27.072594649806543 27.464198152462714 22.577781127283163 62-63 22.6797147717998 27.056511678994806 27.482333586149725 22.781439963055668 64-65 22.513120151548996 26.911571886276036 27.508422729206387 23.066885232968577 66-67 22.57552021114712 27.195886736119018 27.268348296535986 22.960244756197877 68-69 22.648209891301228 26.95228937037324 27.45419679818947 22.94530394013606 70-71 22.487857597560136 26.9783737759384 27.543346252090544 22.99042237441092 72-73 22.659735328498286 27.056848411185282 27.29230438665835 22.99111187365808 74-75 22.55194710234073 27.172779436156823 27.166589912294768 23.108683549207676 76-77 22.607926675763782 26.994536921081195 27.27477827788744 23.12275812526758 78-79 22.576786965577952 26.994601060546053 27.274617929225307 23.153994044650688 80-81 22.468802950160242 27.114121961571787 27.26265299875393 23.154422089514043 82-83 22.62704023628979 27.234931635347902 27.15799634725748 22.980031781104834 84-85 22.540419889006653 27.10625183398782 27.127337683058037 23.22599059394748 86-87 22.475254192716644 27.01764316329427 27.178104069488697 23.328998574500392 88-89 22.520908043094398 26.990826052906534 27.174314615778826 23.313951288220235 90-91 22.43768809903263 27.134108747518006 27.265097590616648 23.163105562832715 92-93 22.485400376602055 26.950265790996603 27.327348900171593 23.236984932229753 94-95 22.330924361932585 26.961697515076782 27.306447138658296 23.400930984332334 96-97 22.396462580408656 26.933808726742548 27.221126248765625 23.448602444083168 98-99 22.13362171259825 26.830833105631736 27.24699786666051 23.78854731510951 100-101 22.18552136473098 26.815457740527805 27.341281842167465 23.65773905257375 102-103 22.24677338404627 26.728903327395088 27.33864515001419 23.68567813854445 104-105 22.112449309779183 26.705829154914447 27.399224874567256 23.78249666073911 106-107 21.99686739237033 26.85297044515547 27.39476275981193 23.755399402662267 108-109 22.104451455958067 26.668215634991228 27.17961570512774 24.047717203922964 110-111 21.959415688538257 26.821275094605863 27.30057404913091 23.918735167724968 112-113 21.94470857432401 26.83862991689129 27.35092785753342 23.86573365125128 114-115 21.83841344619741 26.920808606233393 27.235989936517964 24.00478801105123 116-117 21.682891278796614 26.859571452165586 27.294918069851082 24.16261919918671 118-119 21.628517047468016 26.631988596003147 27.364076447828158 24.375417908700676 120-121 21.57528919886998 26.71814001936384 27.40193567856665 24.304635103199523 122-123 21.633239568336787 26.77754476393959 27.217238562589337 24.37197710513428 124-125 21.404951277104427 26.637836158451396 27.24937180667726 24.70784075776692 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 249.0 1 198.5 2 213.0 3 290.5 4 224.5 5 191.0 6 343.5 7 675.5 8 1158.0 9 1494.5 10 1293.0 11 969.5 12 985.0 13 1124.0 14 1302.0 15 1540.0 16 1805.0 17 2112.5 18 2477.0 19 2888.5 20 3453.0 21 4209.0 22 5163.5 23 6355.0 24 7912.5 25 10530.0 26 14869.5 27 19863.5 28 24519.0 29 30432.5 30 37707.0 31 45113.5 32 52664.5 33 60179.5 34 68265.0 35 74564.0 36 81165.0 37 88925.5 38 92819.0 39 94541.5 40 96384.5 41 99770.0 42 103003.0 43 106313.5 44 110354.0 45 112789.0 46 114382.0 47 115018.0 48 117211.5 49 118287.0 50 115054.0 51 110432.0 52 106920.0 53 105419.5 54 102191.0 55 95192.0 56 87449.5 57 79429.0 58 72548.0 59 64621.0 60 54177.5 61 44021.0 62 35093.0 63 26683.5 64 19065.0 65 14177.5 66 10670.5 67 8142.0 68 6411.0 69 5036.5 70 3849.5 71 3049.5 72 2528.0 73 2274.0 74 1809.5 75 1335.5 76 971.5 77 556.5 78 340.0 79 204.5 80 120.0 81 77.5 82 57.5 83 45.0 84 26.0 85 11.5 86 9.0 87 11.0 88 9.5 89 4.0 90 1.5 91 1.5 92 1.0 93 1.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09274566617653425 2 3.2069732426187504E-5 3 0.0 4 0.09540745396790781 5 0.0 6 0.0 7 0.0013789984943260627 8 0.004682180934223375 9 0.002565578594095 10-11 3.20697324261875E-4 12-13 0.0 14-15 4.810459863928126E-5 16-17 1.603486621309375E-4 18-19 0.001475207691604625 20-21 9.460571065725312E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.6034866213093752E-5 52-53 0.0 54-55 0.0 56-57 0.007023271401335063 58-59 5.612203174582813E-4 60-61 0.0 62-63 0.0 64-65 1.7638352834403127E-4 66-67 0.0 68-69 1.6034866213093752E-5 70-71 0.0 72-73 0.0 74-75 0.0010583011700641875 76-77 0.0 78-79 0.0 80-81 4.810459863928126E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.658827755070625E-4 90-91 1.6034866213093752E-5 92-93 0.0019081490793581564 94-95 0.0 96-97 0.0072477595283183755 98-99 0.0028542061859306877 100-101 0.0016676260861617502 102-103 0.0 104-105 0.0 106-107 0.00115451036734275 108-109 1.6034866213093752E-5 110-111 1.603486621309375E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 7.055341133761251E-4 122-123 0.004040786285699625 124-125 0.08094400464369726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3118205.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.99091978097085 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61070039554633 41.5790196003451 2 12.726139706974193 13.996442554952914 3 4.82746950476152 7.964009648442715 4 2.295229490262552 5.048671231117864 5 1.2574546539762985 3.4574294002509545 6 0.7967746433126622 2.628922229635096 7 0.5023996824533334 1.9339194445043542 8 0.35545086550592064 1.5637256024890196 9 0.2492147907052851 1.2334095507515197 >10 1.2506137644370603 12.326652590234776 >50 0.08304426258282423 3.1227676598165095 >100 0.04404087212295115 4.163526727506152 >500 0.001173893887501707 0.39974757716452786 >1k 1.7608408292625076E-4 0.19621037700684332 >5k 1.173893886175005E-4 0.38554580578166536 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6223 0.19956994488816482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5722 0.1835030089426449 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3122 0.10012170463455738 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.2069732426187504E-5 0.0 4 0.0 0.0 0.0 1.2827892970475001E-4 0.0 5 0.0 3.2069732426187504E-5 0.0 1.92418394557125E-4 0.0 6 0.0 3.2069732426187504E-5 0.0 1.92418394557125E-4 0.0 7 0.0 3.2069732426187504E-5 0.0 2.5655785940950003E-4 0.0 8 0.0 3.2069732426187504E-5 0.0 3.8483678911425E-4 0.0 9 0.0 3.2069732426187504E-5 0.0 4.810459863928125E-4 3.2069732426187504E-5 10-11 0.0 3.2069732426187504E-5 0.0 5.451854512451876E-4 3.2069732426187504E-5 12-13 0.0 3.2069732426187504E-5 0.0 8.177781768677813E-4 3.2069732426187504E-5 14-15 0.0 3.2069732426187504E-5 0.0 8.979525079332501E-4 6.413946485237501E-5 16-17 0.0 3.2069732426187504E-5 0.0 9.460571065725313E-4 6.413946485237501E-5 18-19 0.0 3.2069732426187504E-5 0.0 9.781268389987188E-4 6.413946485237501E-5 20-21 0.0 3.2069732426187504E-5 0.0 0.0010903709024903752 6.413946485237501E-5 22-23 3.2069732426187504E-5 3.2069732426187504E-5 0.0 0.0012667544308344063 1.4431379591784376E-4 24-25 3.2069732426187504E-5 3.2069732426187504E-5 0.0 0.0014752076916046252 1.92418394557125E-4 26-27 3.2069732426187504E-5 3.2069732426187504E-5 0.0 0.0017798701496534062 1.92418394557125E-4 28-29 8.017433106546876E-5 3.2069732426187504E-5 0.0 0.002212811537406938 1.92418394557125E-4 30-31 9.62091972785625E-5 3.2069732426187504E-5 0.0 0.003543705433093719 1.92418394557125E-4 32-33 9.62091972785625E-5 3.2069732426187504E-5 0.0 0.006670504344647001 1.92418394557125E-4 34-35 1.4431379591784376E-4 3.2069732426187504E-5 0.0 0.010695255764133531 1.92418394557125E-4 36-37 1.603486621309375E-4 3.2069732426187504E-5 0.0 0.01845613101127091 1.92418394557125E-4 38-39 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.03274319680713744 1.92418394557125E-4 40-41 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.050349479909114384 1.92418394557125E-4 42-43 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.06858112279340198 1.92418394557125E-4 44-45 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.08997163432166905 1.92418394557125E-4 46-47 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.1151784440086524 2.0845326077021878E-4 48-49 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.14482691163666278 2.5655785940950003E-4 50-51 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.1791415253326834 2.5655785940950003E-4 52-53 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.21361648769083494 3.2069732426187505E-4 54-55 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.2500797734594101 4.0087165532734373E-4 56-57 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.29481705019394167 4.169065215404375E-4 58-59 1.92418394557125E-4 3.2069732426187504E-5 0.0 0.34712278378105355 4.169065215404375E-4 60-61 2.0845326077021878E-4 3.2069732426187504E-5 0.0 0.4044474304928637 4.169065215404375E-4 62-63 2.2448812698331252E-4 3.2069732426187504E-5 0.0 0.4644980044608998 4.810459863928125E-4 64-65 2.5655785940950003E-4 3.2069732426187504E-5 0.0 0.5246127178937883 4.810459863928125E-4 66-67 3.5276705668806253E-4 3.2069732426187504E-5 0.0 0.5943804207869592 5.131157188190001E-4 68-69 3.8483678911425E-4 3.2069732426187504E-5 0.0 0.6809205937390261 5.131157188190001E-4 70-71 4.0087165532734373E-4 3.2069732426187504E-5 0.0 0.7769534074892446 5.451854512451875E-4 72-73 4.48976253966625E-4 3.2069732426187504E-5 0.0 0.8813403865364848 7.055341133761251E-4 74-75 4.810459863928125E-4 3.2069732426187504E-5 0.0 0.9842040532934813 7.055341133761251E-4 76-77 4.810459863928125E-4 4.810459863928125E-5 0.0 1.103695876313456 7.055341133761251E-4 78-79 4.810459863928125E-4 6.413946485237501E-5 0.0 1.2384368571020827 7.055341133761251E-4 80-81 5.451854512451875E-4 6.413946485237501E-5 0.0 1.392082945155947 7.376038458023126E-4 82-83 5.451854512451875E-4 6.413946485237501E-5 0.0 1.560914051513611 7.696735782285E-4 84-85 5.77255183671375E-4 6.413946485237501E-5 0.0 1.7285746126377193 7.696735782285E-4 86-87 6.253597823106563E-4 6.413946485237501E-5 0.0 1.9099770541064491 7.696735782285E-4 88-89 6.4139464852375E-4 6.413946485237501E-5 0.0 2.1161854336068346 7.696735782285E-4 90-91 7.215689795892188E-4 6.413946485237501E-5 0.0 2.350887770367888 7.696735782285E-4 92-93 7.376038458023125E-4 6.413946485237501E-5 0.0 2.606852339727504 7.696735782285E-4 94-95 7.376038458023125E-4 6.413946485237501E-5 0.0 2.865863533667607 8.017433106546876E-4 96-97 7.376038458023125E-4 6.413946485237501E-5 0.0 3.133341136968224 8.017433106546876E-4 98-99 7.696735782285E-4 6.413946485237501E-5 0.0 3.4262500380828076 8.017433106546876E-4 100-101 8.177781768677813E-4 6.413946485237501E-5 0.0 3.7654997025532317 8.017433106546876E-4 102-103 8.33813043080875E-4 6.413946485237501E-5 0.0 4.133708335404503 8.338130430808751E-4 104-105 0.001026231437638 6.413946485237501E-5 0.0 4.511858585307893 8.658827755070625E-4 106-107 0.0010583011700641875 6.413946485237501E-5 0.0 4.895701212716931 8.819176417201563E-4 108-109 0.0010743360362772813 6.413946485237501E-5 0.0 5.297230297559012 8.979525079332501E-4 110-111 0.0011384755011296563 6.413946485237501E-5 0.0 5.748740060387306 8.979525079332501E-4 112-113 0.0011865800997689375 6.413946485237501E-5 0.0 6.232912845691672 8.979525079332501E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5835 0.0 66.61946 1 GTATCAA 10455 0.0 53.490078 1 ATCAACG 13655 0.0 41.354275 3 TCAACGC 13895 0.0 40.725723 4 CAACGCA 14140 0.0 40.05803 5 TATCAAC 14380 0.0 38.647953 2 AACGCAG 14600 0.0 38.592167 6 ACGCAGA 17225 0.0 32.261883 7 CGCAGAG 17445 0.0 31.684494 8 GTACATG 14495 0.0 31.301186 1 TACATGG 14915 0.0 29.593662 2 GCAGAGT 18710 0.0 28.428362 9 ACATGGG 15475 0.0 27.829882 3 GAGTACT 10760 0.0 25.683933 12-13 AGAGTAC 17990 0.0 24.70419 10-11 TGGTATC 2700 0.0 24.267927 2 GTGGTAT 2865 0.0 24.139471 1 GTACTTT 11665 0.0 23.69131 14-15 CAGAGTA 18840 0.0 23.684353 10-11 CATGGGG 8215 0.0 23.493147 4 >>END_MODULE