FastQCFastQC Report
Thu 26 May 2016
SRR1364576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709153
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41120.15178175614297162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30450.1123967527858338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44500.061.7748341
GTATCAA80750.052.685751
ATCAACG98550.042.5031973
TCAACGC99250.042.263384
CAACGCA101600.041.3443875
AACGCAG102500.040.9753156
TATCAAC106700.039.3124662
GTACATG142750.035.638521
ACGCAGA120450.034.81967
CGCAGAG121850.033.7397658
TACATGG149250.033.087912
ACATGGG151200.031.598713
GCAGAGT132050.029.5115789
CATGGGG71250.027.0561314
CATGGGA86950.026.5501964
GAGTACT55800.024.7436412-13
CAGAGTA129500.024.51491210-11
AGTACAT102900.024.400722
GAGTACA111800.024.0029371
AGAGTAC124200.022.78214610-11
Produced by FastQC (version 0.11.5)