##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364576_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2709153 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.813412531518154 31.0 30.0 34.0 22.0 34.0 2 28.726066412638932 31.0 28.0 34.0 18.0 34.0 3 28.839752129171 31.0 28.0 34.0 20.0 34.0 4 32.181327152803846 35.0 32.0 37.0 22.0 37.0 5 32.23727009880948 35.0 33.0 37.0 22.0 37.0 6 32.284976891301454 35.0 33.0 37.0 23.0 37.0 7 32.03248210787652 35.0 32.0 37.0 19.0 37.0 8 32.006172039748215 35.0 32.0 37.0 19.0 37.0 9 33.118011791877386 37.0 33.0 39.0 17.0 39.0 10-11 33.05970186992023 37.0 32.5 39.0 17.0 39.0 12-13 33.01801540924414 37.0 33.0 39.0 17.0 39.0 14-15 33.790563323666106 38.0 32.5 41.0 15.0 41.0 16-17 33.92275556234735 38.0 33.0 41.0 17.0 41.0 18-19 33.7329013902131 38.5 32.0 41.0 16.0 41.0 20-21 33.46248329274869 38.5 32.0 41.0 12.0 41.0 22-23 33.374151625987906 38.0 32.0 40.5 12.0 41.0 24-25 33.20728674238775 38.0 32.0 40.5 10.5 41.0 26-27 33.13566601812448 38.0 31.5 40.0 11.0 41.0 28-29 33.19762523563638 38.0 32.0 40.0 10.5 41.0 30-31 33.14465775834735 38.0 31.5 40.0 10.5 41.0 32-33 33.139211037545685 38.0 32.0 40.0 9.0 41.0 34-35 32.66905412872584 38.0 31.0 40.0 9.0 41.0 36-37 32.53049329439865 38.0 30.5 40.0 8.5 41.0 38-39 32.33979679257686 38.0 30.0 40.0 8.0 41.0 40-41 32.54938554596215 38.0 30.5 40.0 8.0 41.0 42-43 32.42711873415787 38.0 31.0 40.0 4.5 41.0 44-45 32.27689244572012 38.0 30.5 40.0 2.0 41.0 46-47 32.2158661766242 38.0 31.0 40.0 2.0 41.0 48-49 32.0546552741761 37.5 30.0 40.0 2.0 41.0 50-51 31.889614392394968 37.0 30.0 40.0 2.0 41.0 52-53 31.82020487584127 37.0 30.0 40.0 2.0 41.0 54-55 31.829296462768994 37.0 30.0 40.0 2.0 41.0 56-57 31.68837252824038 37.0 30.0 40.0 2.0 41.0 58-59 31.48083515401308 36.5 30.0 40.0 2.0 41.0 60-61 31.258870023213895 36.0 30.0 40.0 2.0 41.0 62-63 30.96355724464436 36.0 30.0 39.5 2.0 41.0 64-65 30.67386375003553 35.0 29.0 39.0 2.0 41.0 66-67 30.415553680430747 35.0 29.0 39.0 2.0 40.5 68-69 30.36765236219586 35.0 29.5 39.0 2.0 40.0 70-71 30.11977950304025 35.0 29.0 38.0 2.0 40.0 72-73 29.829220424243296 35.0 29.0 37.0 2.0 40.0 74-75 29.522058370272923 35.0 29.0 37.0 2.0 39.0 76-77 29.218910486044898 34.5 29.0 36.0 2.0 39.0 78-79 28.915336822984898 34.0 29.0 36.0 2.0 38.5 80-81 28.624641723815525 34.0 29.0 35.5 2.0 37.0 82-83 28.387950588246582 34.0 29.0 35.0 2.0 37.0 84-85 28.15752746338062 34.0 28.0 35.0 2.0 36.0 86-87 27.974132874739816 34.0 28.0 35.0 2.0 36.0 88-89 27.791345302387867 34.0 28.0 35.0 2.0 36.0 90-91 27.663611468233796 34.0 27.5 35.0 2.0 35.5 92-93 27.54165157892522 34.0 27.0 35.0 2.0 35.0 94-95 27.4226018242602 34.0 27.0 35.0 2.0 35.0 96-97 27.31906152956293 34.0 27.0 35.0 2.0 35.0 98-99 27.23270077400575 34.0 27.0 35.0 2.0 35.0 100-101 27.14177659954975 34.0 26.5 35.0 2.0 35.0 102-103 25.97123049159645 31.5 24.5 34.0 2.0 35.0 104-105 26.84763005264007 33.5 25.5 35.0 2.0 35.0 106-107 26.99505620391318 34.0 26.0 35.0 2.0 35.0 108-109 26.949812358327492 34.0 26.0 35.0 2.0 35.0 110-111 26.878800680507894 34.0 25.5 35.0 2.0 35.0 112-113 26.79050777124806 34.0 25.0 35.0 2.0 35.0 114-115 26.66970285546811 34.0 25.0 35.0 2.0 35.0 116-117 26.560593661561384 34.0 25.0 35.0 2.0 35.0 118-119 26.413045331880483 34.0 25.0 35.0 2.0 35.0 120-121 26.23904371587725 34.0 25.0 35.0 2.0 35.0 122-123 26.00842292775639 34.0 24.0 35.0 2.0 35.0 124-125 24.53290954774426 32.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8056651719008734 1101 2 0.8342661727083573 1101 3 0.822540620678236 1101 4 0.8853794186906256 1101 5 0.8336559778063304 1101 6 0.9883079263916912 1101 7 1.049156663305837 1101 8 0.9601949830324514 1101 9 1.345431504865104 1101 10-11 1.389757133771262 1101 12-13 1.1351078583764362 1101 14-15 1.3377805220527108 1101 16-17 1.1146357197414716 1101 18-19 1.4561562152895533 1101 20-21 1.842381324438719 1101 22-23 1.7549906528108181 1101 24-25 1.8976667684438482 1101 26-27 2.0388981555511734 1101 28-29 1.8735044241142376 1101 30-31 1.8739277764676032 1101 32-33 1.7255711498706319 1101 34-35 2.176385734724022 1101 36-37 2.065595856974376 1101 38-39 1.889506684681443 1101 40-41 1.761868907457739 1101 42-43 1.7192896278757388 1101 44-45 1.6930861252887084 1101 46-47 1.6133330891078685 1101 48-49 1.5502472390345048 1101 50-51 1.4894903429846238 1101 52-53 1.3495451695086125 1101 54-55 1.3279307128787323 1101 56-57 1.285684285678812 1101 58-59 1.336974903322215 1101 60-61 1.2408827147208719 1101 62-63 1.2681133413699541 1101 64-65 1.292299634001683 1101 66-67 1.2099701414522421 1101 68-69 1.089209440021186 1101 70-71 0.9714712331592601 1101 72-73 0.8780206505381081 1101 74-75 0.8713908745639785 1101 76-77 1.0492560898871943 1101 78-79 1.0226810558042985 1101 80-81 1.0899334074245566 1101 82-83 0.9209315698944991 1101 84-85 1.0406366870996102 1101 86-87 1.0532213080179567 1101 88-89 0.9848115432870692 1101 90-91 1.081365842505786 1101 92-93 0.9294326410658229 1101 94-95 1.0227150092745276 1101 96-97 0.8865803601590336 1101 98-99 0.9842572915683689 1101 100-101 0.9837368052919544 1101 102-103 0.9777797333128753 1101 104-105 0.9608905180812215 1101 106-107 0.8838032968251888 1101 108-109 0.996658011352018 1101 110-111 0.8126970359135868 1101 112-113 0.8408111549318278 1101 114-115 0.9393276332938036 1101 116-117 1.0096651012183315 1101 118-119 1.0441882057443657 1101 120-121 1.1357175186111874 1101 122-123 1.160146921022129 1101 124-125 1.4537116542878188 1102 1 0.2148847484184202 1102 2 0.5521378799115446 1102 3 0.5819068346440588 1102 4 0.709835228537365 1102 5 0.649443850136592 1102 6 -0.0924481298428077 1102 7 -0.4788308822691114 1102 8 0.3493053372582011 1102 9 0.7100803035095993 1102 10-11 0.667247139950959 1102 12-13 0.8145104789647348 1102 14-15 0.9897178649389957 1102 16-17 0.8493600057006176 1102 18-19 1.0624800022183152 1102 20-21 1.511252825813699 1102 22-23 1.2708926397924856 1102 24-25 1.4172244487089998 1102 26-27 0.9642397903799278 1102 28-29 1.4778941774088779 1102 30-31 1.3545517414171684 1102 32-33 1.2706894089664118 1102 34-35 1.6450025085353062 1102 36-37 1.6937157362703132 1102 38-39 1.8902147797910516 1102 40-41 1.579536507745658 1102 42-43 1.3660577491829855 1102 44-45 1.4883785746904792 1102 46-47 1.2643657212434007 1102 48-49 0.9816656548167018 1102 50-51 1.3647026866648844 1102 52-53 1.1934038641989453 1102 54-55 1.0680086536462667 1102 56-57 1.1225231231591408 1102 58-59 1.1980220113146274 1102 60-61 1.161152708482991 1102 62-63 1.0352573888738448 1102 64-65 1.077930339885775 1102 66-67 1.0972067074036964 1102 68-69 0.9602199425935503 1102 70-71 0.9971213921124615 1102 72-73 1.088218711098147 1102 74-75 0.6933224401990543 1102 76-77 0.9094704300283745 1102 78-79 0.8677856899937488 1102 80-81 0.5507874999512445 1102 82-83 0.7560676577378089 1102 84-85 0.4279008907614106 1102 86-87 0.8544368945228982 1102 88-89 1.1097002954420212 1102 90-91 0.5927034858673181 1102 92-93 0.8932777884293657 1102 94-95 0.8766424370110073 1102 96-97 1.0336536478513594 1102 98-99 1.0891093098121658 1102 100-101 1.0869288032535493 1102 102-103 0.9660919516779352 1102 104-105 1.2171702180132655 1102 106-107 1.1707588551445163 1102 108-109 0.7959710203010602 1102 110-111 0.9875446466698925 1102 112-113 0.7242039578296229 1102 114-115 0.7736233165501254 1102 116-117 0.6570126446986322 1102 118-119 1.028116089260628 1102 120-121 1.375523506733277 1102 122-123 1.2064617232706745 1102 124-125 1.4938828844495795 1103 1 0.6210739220584074 1103 2 0.579906812704369 1103 3 0.44833134831616306 1103 4 0.538161985959313 1103 5 0.560073794066227 1103 6 0.5881226481095752 1103 7 0.6296630429077297 1103 8 0.6353412413898241 1103 9 0.826596110550355 1103 10-11 1.0446421144355682 1103 12-13 0.9419258775722597 1103 14-15 1.258992761821787 1103 16-17 1.1523108362242027 1103 18-19 1.171736510008273 1103 20-21 1.6570148695252556 1103 22-23 1.5744844924868175 1103 24-25 1.6482832278582862 1103 26-27 1.5228780009117813 1103 28-29 1.5096375091788303 1103 30-31 1.426669073327865 1103 32-33 1.3345607473271954 1103 34-35 1.7397927084997704 1103 36-37 1.7045157909971138 1103 38-39 1.8035394775755478 1103 40-41 1.4646498936173842 1103 42-43 1.2220820164860342 1103 44-45 1.0681142274823614 1103 46-47 0.9774490575706309 1103 48-49 0.9883526479970186 1103 50-51 1.0040775165663973 1103 52-53 1.0201776500586597 1103 54-55 0.8273051361201524 1103 56-57 0.7945249357385293 1103 58-59 0.7591501192878596 1103 60-61 0.6667951185098069 1103 62-63 0.7363374927760304 1103 64-65 0.8631040630458955 1103 66-67 0.8797743863030192 1103 68-69 0.9272534396315635 1103 70-71 0.8661350939921206 1103 72-73 0.9242750147453087 1103 74-75 0.82288175652738 1103 76-77 0.7819410992653637 1103 78-79 0.8973429639097787 1103 80-81 0.85046099696957 1103 82-83 0.9098774825775813 1103 84-85 0.8540841118769045 1103 86-87 0.7289640022554735 1103 88-89 0.8049111093014858 1103 90-91 0.8142008841293453 1103 92-93 0.9237262800217643 1103 94-95 0.8564253851814705 1103 96-97 0.8498681621681747 1103 98-99 0.798234734104863 1103 100-101 0.7586310210180116 1103 102-103 0.6311942248582092 1103 104-105 0.665002578781035 1103 106-107 0.6851592767180996 1103 108-109 0.7837259588785841 1103 110-111 0.8240453595456003 1103 112-113 0.7992905388855789 1103 114-115 0.8355944732592526 1103 116-117 0.8744086083997971 1103 118-119 0.9598100709360082 1103 120-121 1.0356194090636457 1103 122-123 1.0312333299010845 1103 124-125 1.0875070638550746 1104 1 0.7737639847114295 1104 2 0.8491146312672946 1104 3 0.7189525720702079 1104 4 0.9160292854103673 1104 5 0.7915665198467394 1104 6 0.8408000489358187 1104 7 0.8108260432081593 1104 8 0.9319078143973414 1104 9 1.1791168500470874 1104 10-11 1.0975499605091912 1104 12-13 1.152230359974041 1104 14-15 1.3007376824148622 1104 16-17 1.3871323274881178 1104 18-19 1.5842312582278453 1104 20-21 1.8175923869554111 1104 22-23 1.5467146603250939 1104 24-25 1.9937178623286158 1104 26-27 2.0863748463903065 1104 28-29 1.9055936382511902 1104 30-31 1.7648863226495948 1104 32-33 1.8048266629556835 1104 34-35 2.0631469875641315 1104 36-37 2.1722941468704207 1104 38-39 2.079901688698847 1104 40-41 1.776543061180547 1104 42-43 1.8318824718513653 1104 44-45 1.6624801749956362 1104 46-47 1.5750039149351522 1104 48-49 1.5337067647597777 1104 50-51 1.366643055908412 1104 52-53 1.4092797884109807 1104 54-55 1.3202936207642395 1104 56-57 1.311128935954926 1104 58-59 1.3152622420661473 1104 60-61 1.281350780475865 1104 62-63 1.4168946534419433 1104 64-65 1.370439318291055 1104 66-67 1.4010025432129787 1104 68-69 1.4635553574378903 1104 70-71 1.390071356658261 1104 72-73 1.3437321561882705 1104 74-75 1.4124607853192188 1104 76-77 1.3420143306671513 1104 78-79 1.4225021863815286 1104 80-81 1.4407657498740214 1104 82-83 1.4391757123390896 1104 84-85 1.3722414880240947 1104 86-87 1.3667196128835783 1104 88-89 1.3931592649913078 1104 90-91 1.402643053537684 1104 92-93 1.3781663510200808 1104 94-95 1.3946999580462531 1104 96-97 1.2728760251626028 1104 98-99 1.356596662063673 1104 100-101 1.3752139565589516 1104 102-103 1.403250580347052 1104 104-105 1.4351366099728686 1104 106-107 1.4297845326884406 1104 108-109 1.5541248812420179 1104 110-111 1.5425297849231256 1104 112-113 1.5419165787381033 1104 114-115 1.5557400439795863 1104 116-117 1.551380716697551 1104 118-119 1.5179374097768523 1104 120-121 1.5478657264864353 1104 122-123 1.6177717936531515 1104 124-125 1.914674912484088 1105 1 0.14730361175935514 1105 2 0.4365020443127854 1105 3 0.43693729228495215 1105 4 0.6147039456024714 1105 5 0.4345430163187203 1105 6 0.41049936611596394 1105 7 0.501621005008694 1105 8 0.34580202360081813 1105 9 0.4125563681571194 1105 10-11 0.5472507017140629 1105 12-13 0.4612047061686937 1105 14-15 0.5363551039333032 1105 16-17 0.4951448873861537 1105 18-19 0.6289040529475614 1105 20-21 1.0588976480147423 1105 22-23 1.178370302667048 1105 24-25 1.2871931234591472 1105 26-27 1.1677789347652734 1105 28-29 0.9533461712351539 1105 30-31 0.8655198712479972 1105 32-33 0.9085190166549424 1105 34-35 1.5436194789665834 1105 36-37 1.429141640942916 1105 38-39 1.5285462307681001 1105 40-41 1.1614031151510815 1105 42-43 0.7723521152043702 1105 44-45 0.9564015830712762 1105 46-47 0.9799702011498823 1105 48-49 0.8810692573726513 1105 50-51 0.8067115514975001 1105 52-53 0.8996051068585125 1105 54-55 0.7586256606084518 1105 56-57 0.7104768750351553 1105 58-59 0.7042962548966116 1105 60-61 0.728804410670886 1105 62-63 0.7574463206188895 1105 64-65 0.7032855877022932 1105 66-67 0.5008567230815437 1105 68-69 0.3715531074788281 1105 70-71 0.6171531739986094 1105 72-73 0.6218851961250458 1105 74-75 0.5958921152871071 1105 76-77 0.6711631198901351 1105 78-79 0.7746743224898154 1105 80-81 0.5975381978437184 1105 82-83 0.5786644874642235 1105 84-85 0.6300963371842769 1105 86-87 0.6008326946866802 1105 88-89 0.7948400413695857 1105 90-91 0.6762718245331492 1105 92-93 0.6244336262199752 1105 94-95 0.6919749443400178 1105 96-97 0.8173676839378032 1105 98-99 0.6418282440839356 1105 100-101 0.6728806920534822 1105 102-103 0.612586628820857 1105 104-105 0.8157540670919268 1105 106-107 0.7009362126816079 1105 108-109 0.6633032901792006 1105 110-111 0.8081246356497331 1105 112-113 0.8529544791560575 1105 114-115 0.766409406799724 1105 116-117 0.7732281039681972 1105 118-119 0.8088111201444654 1105 120-121 0.8441316488628487 1105 122-123 0.9911117724351826 1105 124-125 1.1191627263925348 1106 1 0.6301571366007757 1106 2 0.8402762169204152 1106 3 0.6684773235495989 1106 4 0.736892761941597 1106 5 0.6044805161367819 1106 6 0.638474761945595 1106 7 0.41463641318117084 1106 8 0.6591785762805813 1106 9 0.7639856631888335 1106 10-11 0.9743572373059806 1106 12-13 0.9383515597633973 1106 14-15 1.293501811975645 1106 16-17 1.0606104657093312 1106 18-19 1.0498066231110812 1106 20-21 1.4301746370604747 1106 22-23 1.3716884888517882 1106 24-25 1.5853609395042554 1106 26-27 1.7704653363908065 1106 28-29 1.6029026732044827 1106 30-31 1.4444860710797371 1106 32-33 1.373910421497179 1106 34-35 1.6434055461127954 1106 36-37 1.552761528052507 1106 38-39 1.68994688109602 1106 40-41 1.49050316492999 1106 42-43 1.337760200346679 1106 44-45 1.1879670568878637 1106 46-47 0.9600413180313367 1106 48-49 1.0427830490406507 1106 50-51 0.9492027965232452 1106 52-53 0.9582819486052578 1106 54-55 0.7275112535450816 1106 56-57 0.7624801412300357 1106 58-59 0.6751248844629458 1106 60-61 0.7674779920448422 1106 62-63 0.7159514688057449 1106 64-65 0.7557019897062602 1106 66-67 0.6181926200770498 1106 68-69 0.6867043218221411 1106 70-71 0.8051747390357704 1106 72-73 0.8488552618273779 1106 74-75 0.8389204193983808 1106 76-77 0.685158502969383 1106 78-79 0.7701767837801654 1106 80-81 0.7591118225922777 1106 82-83 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86-87 -2.61859276811321 2216 88-89 -2.645808506025144 2216 90-91 -2.6821952575299086 2216 92-93 -2.647229808236329 2216 94-95 -2.698527836440544 2216 96-97 -2.686109931487195 2216 98-99 -2.728530753944348 2216 100-101 -2.7657822120234243 2216 102-103 -2.6864488904043853 2216 104-105 -2.7303060236798764 2216 106-107 -2.7999241085960556 2216 108-109 -2.761224881334435 2216 110-111 -2.8546242411740366 2216 112-113 -2.8305671591089947 2216 114-115 -2.9748763246447574 2216 116-117 -3.0670730381694327 2216 118-119 -3.126974887286245 2216 120-121 -3.273245928639348 2216 122-123 -3.2769326120885367 2216 124-125 -3.447866568634719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 155236.0 3 25774.0 4 16133.0 5 12931.0 6 12305.0 7 13350.0 8 13875.0 9 14708.0 10 15847.0 11 17091.0 12 18304.0 13 20942.0 14 24203.0 15 21388.0 16 15940.0 17 14157.0 18 13122.0 19 12358.0 20 12086.0 21 12950.0 22 15695.0 23 20503.0 24 27441.0 25 35404.0 26 43070.0 27 49362.0 28 56760.0 29 67139.0 30 76484.0 31 87404.0 32 109062.0 33 138066.0 34 181105.0 35 249249.0 36 394384.0 37 529121.0 38 165717.0 39 487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.56098313717006 18.214868735136395 12.755620758727673 23.468527368965866 2 15.308550100793317 19.663574391963998 40.65748766430824 24.370387842934445 3 19.49423741176896 25.318141161539632 28.262541164640474 26.925080262050937 4 12.40929102105696 16.14108554717753 36.99449747465612 34.45512595710939 5 13.957920167043468 38.20104136363703 33.387466423701504 14.453572045617987 6 32.47800307774196 35.956870270850885 17.571699243557614 13.993427407849534 7 29.329696997438866 32.52868081185579 20.141205793823 18.000416396882336 8 26.860904496719083 35.257144945302095 19.30027576884731 18.58167478913151 9 27.337744169066614 16.29560671015981 19.289679249754347 37.07696987101923 10-11 25.483278353049826 26.823088249353212 26.77501049220919 20.918622905387775 12-13 26.15313770618276 23.99124790287424 27.24116992394055 22.614444467002453 14-15 23.09340567349206 25.131332202033867 25.19765591009215 26.577606214381927 16-17 23.178358400454883 28.9019696166918 25.361034856437115 22.558637126416198 18-19 22.89309596938654 27.220681988531208 27.65938557872458 22.226836463357667 20-21 23.62983977098011 26.4641455036758 27.776450893063142 22.12956383228095 22-23 23.451319757099874 26.319747871707804 27.483825791398388 22.74510657979393 24-25 23.250849202682527 26.493683977179366 27.57337991619736 22.682086903940746 26-27 23.00801105772949 26.902508534095865 27.385508917860214 22.703971490314427 28-29 22.79076909504627 26.714470301167697 27.71074642363246 22.78401418015358 30-31 22.929170782412513 26.484639962674063 27.607814084931277 22.978375169982147 32-33 22.787784128610735 26.657169641685417 27.47698020446167 23.078066025242176 34-35 23.40384768368748 26.534568926422388 27.352143107505423 22.709440282384712 36-37 23.225788589163944 26.29027390547174 27.333143217334886 23.15079428802943 38-39 22.970408094337973 26.598866878319534 27.580631289558028 22.850093737784466 40-41 23.205467328995056 26.401449792516264 27.497470487170972 22.89561239131771 42-43 23.040988972877113 26.448612293166462 27.25057647693548 23.25982225702095 44-45 23.149485225914525 26.76009358689783 26.98119494040065 23.10922624678699 46-47 23.136255564221983 26.89692951290287 26.565337134913396 23.401477787961753 48-49 23.403251397669028 26.89821314348848 26.4467132048687 23.251822253973792 50-51 23.175006469088462 26.90144136089688 26.444194006947857 23.479358163066802 52-53 23.60916862207487 26.806404067987298 26.340059051666703 23.244368258271127 54-55 23.329196692385462 26.652575137440405 26.520557038113395 23.49767113206074 56-57 23.238832875697966 27.258715236000132 26.427551956241235 23.074899932060667 58-59 23.439401833530166 26.729775126511292 26.423147077187252 23.407675962771286 60-61 23.480496057277815 26.68736771153788 26.80519534211725 23.026940889067056 62-63 23.33444465120126 26.729908406732 26.687320195414728 23.24832674665202 64-65 23.116856651219077 26.61654229714379 26.566648047848275 23.699953003788863 66-67 23.10645888529298 27.18528956599015 26.235194562026653 23.473056986690217 68-69 23.096883497724587 26.81104964706321 26.686060238212722 23.406006616999484 70-71 22.903010401340087 26.88555203733822 26.72106778921506 23.490369772106632 72-73 23.290700874521914 26.67811432261975 26.61245919165373 23.418725611204604 74-75 23.015426411027065 26.867756613922623 26.445908822039364 23.67090815301095 76-77 23.191676170503918 26.826900839739924 26.564035683363578 23.417387306392577 78-79 23.14710759664799 26.88340966493763 26.52993727673813 23.439545461676246 80-81 22.883202996164542 27.203755098394705 26.59121869974624 23.321823205694514 82-83 23.121913766803274 27.12502180146044 26.33380181480432 23.41926261693196 84-85 23.094302905742126 26.82463854939164 26.32293192743267 23.758126617433568 86-87 23.13469460377366 26.700734418636156 26.69560339954009 23.46896757805009 88-89 23.03927839205738 26.72752178743643 26.543874736799943 23.689325083706247 90-91 23.016339782411897 27.01784757018745 26.717840629342643 23.24797201805801 92-93 23.1163822904812 26.678968737507052 26.665347620863418 23.539301351148325 94-95 23.030389289566525 26.682435329928172 26.566154821985972 23.721020558519335 96-97 23.211325363876213 26.71010421356388 26.474882107448305 23.603688315111604 98-99 22.92307132944881 26.517643232975647 26.50033137987466 24.058954057700888 100-101 22.69704116630164 26.605933171621803 26.812305481961758 23.884720180114797 102-103 23.304460840712945 26.423609150166122 26.600214162876735 23.671715846244197 104-105 23.003965685479262 26.451013255064243 26.858194122768115 23.686826936688377 106-107 22.862361148868935 26.600561098188862 26.883657115521224 23.65342063742098 108-109 22.968322571667233 26.70927038819882 26.36875805833041 23.95364898180354 110-111 22.57195652045523 26.73182520183557 26.855849983168067 23.840368294541136 112-113 22.852899042615903 26.57568620155451 26.91414992065786 23.65726483517173 114-115 22.476120765420042 26.79226680811309 26.693841211625923 24.037771214840948 116-117 22.425090055821876 26.716634313381345 26.847560842816925 24.010714787979857 118-119 22.646081635108832 26.812439164565454 26.383301349167066 24.158177851158648 120-121 22.370630667708813 26.75360652454965 26.762520751415803 24.11324205632573 122-123 22.54826508506533 26.82760995779862 26.702091022544682 23.922033934591365 124-125 22.50305929428279 26.68946976970515 26.59663410249656 24.210836833515504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 490.0 1 538.0 2 683.5 3 783.5 4 668.5 5 501.0 6 499.5 7 678.5 8 957.0 9 1123.0 10 1117.0 11 1104.0 12 1207.5 13 1363.0 14 1539.0 15 1765.0 16 1992.5 17 2190.5 18 2404.5 19 2683.5 20 3090.0 21 3680.0 22 4529.5 23 5614.0 24 6782.5 25 8672.0 26 11615.0 27 14691.5 28 17454.0 29 20782.0 30 25424.5 31 30403.5 32 35493.5 33 41021.5 34 47423.0 35 52001.0 36 56593.5 37 63412.0 38 67785.0 39 68793.0 40 69151.5 41 73685.5 42 79766.5 43 85442.0 44 90072.5 45 92226.5 46 93263.5 47 96676.5 48 105802.5 49 111281.0 50 111947.0 51 111140.5 52 107064.5 53 105322.5 54 103701.5 55 97441.0 56 90024.0 57 81423.0 58 72806.0 59 65080.0 60 55610.0 61 44570.5 62 35579.0 63 28385.5 64 22251.0 65 17770.0 66 13448.0 67 10401.0 68 7606.5 69 4992.5 70 3668.0 71 2883.5 72 2262.5 73 1795.0 74 1414.5 75 1122.5 76 783.5 77 489.5 78 356.0 79 259.0 80 189.5 81 134.5 82 106.0 83 95.0 84 65.5 85 53.0 86 45.0 87 34.0 88 29.5 89 23.5 90 22.5 91 20.5 92 15.5 93 13.5 94 8.0 95 7.0 96 5.0 97 3.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013103726515261411 2 0.00531531441745815 3 0.00789914781483364 4 0.007936059720510433 5 0.014654026553686706 6 8.85885736243025E-4 7 0.00734546922968175 8 0.0 9 0.0024730976803451115 10-11 0.0 12-13 0.003248247699557758 14-15 0.017293227809577386 16-17 0.027979224503008875 18-19 0.005481417993003717 20-21 0.00804679543754081 22-23 0.0020670667179003917 24-25 0.010649084787754698 26-27 0.01696102065848625 28-29 5.352226323134942E-4 30-31 0.0016610357554556717 32-33 0.00865584188120789 34-35 0.008286722824439964 36-37 0.010353789542340355 38-39 0.0 40-41 0.023162220812187426 42-43 0.008914225220945439 44-45 0.01625969445062719 46-47 0.003838838190386442 48-49 0.02308839700083384 50-51 0.004281781058507954 52-53 0.0 54-55 0.01600131111088964 56-57 0.004374060822699936 58-59 2.768392925759453E-4 60-61 0.004097221530123991 62-63 0.02425112202965281 64-65 0.01565064800696011 66-67 0.0020486107650619957 68-69 0.019323382621800986 70-71 0.002916040548466624 72-73 0.011128939561553003 74-75 1.8455952838396355E-5 76-77 0.005887448955448437 78-79 0.0011258131231421777 80-81 0.0033036155580729474 82-83 0.0016794917082940683 84-85 0.0 86-87 0.0051492108419125835 88-89 0.015669103959798505 90-91 0.0202646362165592 92-93 0.004577076303922296 94-95 0.022756189849742706 96-97 0.016112046827920015 98-99 9.781655004350068E-4 100-101 0.00789914781483364 102-103 0.0 104-105 0.0013841964628797266 106-107 5.536785851518906E-5 108-109 0.0 110-111 6.275023965054761E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.8455952838396355E-5 122-123 0.0 124-125 0.0222763350759444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2709153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.37708775810928 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53680940340712 37.29787666879608 2 12.273971181373074 12.121059043263251 3 4.764567560048967 7.057814116259353 4 2.2857215623326343 4.514490966956046 5 1.3456462175227644 3.3222045686994677 6 0.8094658290361392 2.398143916650488 7 0.5447826916916393 1.882984794372995 8 0.380800903976605 1.5042271723216138 9 0.286310784505424 1.2723473459355694 >10 1.5560425729150527 14.04208139514175 >50 0.12737805846187014 4.329038996640319 >100 0.08236742316964449 7.42728562034867 >500 0.004218370446955815 1.392594737029642 >1k 0.0019174411122479067 1.4378506575846377 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4112 0.15178175614297162 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3045 0.1123967527858338 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.536785851518906E-5 0.0 12-13 0.0 0.0 0.0 7.382381135358542E-5 0.0 14-15 0.0 0.0 0.0 1.1073571703037812E-4 0.0 16-17 0.0 0.0 0.0 1.2919166986877448E-4 0.0 18-19 0.0 0.0 0.0 1.4764762270717084E-4 0.0 20-21 0.0 0.0 0.0 2.030154812223599E-4 0.0 22-23 0.0 0.0 0.0 4.983107266367016E-4 0.0 24-25 0.0 0.0 0.0 9.227976419198178E-4 0.0 26-27 0.0 0.0 0.0 0.0011811809816573667 0.0 28-29 0.0 0.0 0.0 0.0016979476611324647 0.0 30-31 0.0 0.0 0.0 0.003155967935365777 0.0 32-33 0.0 0.0 0.0 0.006367303729246742 0.0 34-35 0.0 0.0 0.0 0.011018203844522624 0.0 36-37 0.0 0.0 0.0 0.02015390049952882 0.0 38-39 0.0 0.0 0.0 0.03558307707242817 0.0 40-41 0.0 0.0 0.0 0.05394675014663254 0.0 42-43 0.0 0.0 0.0 0.07273491013612003 0.0 44-45 0.0 0.0 0.0 0.09519580474044839 0.0 46-47 0.0 0.0 0.0 0.12326730900764926 0.0 48-49 0.0 0.0 0.0 0.15477162050279183 0.0 50-51 0.0 0.0 0.0 0.19103756783024067 0.0 52-53 0.0 0.0 0.0 0.22765417826161904 0.0 54-55 0.0 0.0 0.0 0.2667438863733425 0.0 56-57 0.0 0.0 0.0 0.31221935416715113 0.0 58-59 0.0 0.0 0.0 0.36788250792775457 0.0 60-61 0.0 0.0 0.0 0.4317770166542827 0.0 62-63 0.0 3.691190567679271E-5 0.0 0.4976647682873577 0.0 64-65 0.0 3.691190567679271E-5 0.0 0.5638847270715238 0.0 66-67 0.0 3.691190567679271E-5 0.0 0.6423409825875468 0.0 68-69 0.0 3.691190567679271E-5 0.0 0.7318523538537691 0.0 70-71 0.0 3.691190567679271E-5 0.0 0.837457315995073 0.0 72-73 0.0 3.691190567679271E-5 0.0 0.9476208984874608 0.0 74-75 0.0 3.691190567679271E-5 0.0 1.0571754345361817 0.0 76-77 0.0 3.691190567679271E-5 0.0 1.1799259768643557 0.0 78-79 0.0 3.691190567679271E-5 0.0 1.3251374137968583 0.0 80-81 0.0 3.691190567679271E-5 0.0 1.4929204810507195 0.0 82-83 0.0 3.691190567679271E-5 0.0 1.6750438236600147 0.0 84-85 0.0 3.691190567679271E-5 0.0 1.8520733232859126 0.0 86-87 0.0 7.382381135358542E-5 0.0 2.0324987182340752 0.0 88-89 0.0 7.382381135358542E-5 0.0 2.2421398865254196 0.0 90-91 0.0 7.382381135358542E-5 0.0 2.490870762928487 0.0 92-93 0.0 7.382381135358542E-5 0.0 2.7543479456494335 0.0 94-95 0.0 1.1073571703037812E-4 0.0 3.020316682003563 0.0 96-97 0.0 1.1073571703037812E-4 0.0 3.293354048294799 1.8455952838396355E-5 98-99 3.691190567679271E-5 1.1073571703037812E-4 0.0 3.5926911473807497 3.691190567679271E-5 100-101 3.691190567679271E-5 1.1073571703037812E-4 0.0 3.9357688546936993 3.691190567679271E-5 102-103 3.691190567679271E-5 1.4764762270717084E-4 0.0 4.316422881985624 3.691190567679271E-5 104-105 3.691190567679271E-5 1.4764762270717084E-4 0.0 4.700362068882784 3.691190567679271E-5 106-107 3.691190567679271E-5 1.4764762270717084E-4 0.0 5.082197277156366 3.691190567679271E-5 108-109 3.691190567679271E-5 1.4764762270717084E-4 0.0 5.478298936974028 3.691190567679271E-5 110-111 3.691190567679271E-5 1.4764762270717084E-4 0.0 5.930580517231769 3.691190567679271E-5 112-113 3.691190567679271E-5 1.4764762270717084E-4 0.0 6.425292333064984 3.691190567679271E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4450 0.0 61.774834 1 GTATCAA 8075 0.0 52.68575 1 ATCAACG 9855 0.0 42.503197 3 TCAACGC 9925 0.0 42.26338 4 CAACGCA 10160 0.0 41.344387 5 AACGCAG 10250 0.0 40.975315 6 TATCAAC 10670 0.0 39.312466 2 GTACATG 14275 0.0 35.63852 1 ACGCAGA 12045 0.0 34.8196 7 CGCAGAG 12185 0.0 33.739765 8 TACATGG 14925 0.0 33.08791 2 ACATGGG 15120 0.0 31.59871 3 GCAGAGT 13205 0.0 29.511578 9 CATGGGG 7125 0.0 27.056131 4 CATGGGA 8695 0.0 26.550196 4 GAGTACT 5580 0.0 24.74364 12-13 CAGAGTA 12950 0.0 24.514912 10-11 AGTACAT 10290 0.0 24.40072 2 GAGTACA 11180 0.0 24.002937 1 AGAGTAC 12420 0.0 22.782146 10-11 >>END_MODULE