FastQCFastQC Report
Thu 26 May 2016
SRR1364576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709153
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73610.27170853768687114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62310.2299980842720954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.1457651155176544No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA28440.10497745974479845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.066.313421
GTATCAA100100.053.6610341
ATCAACG132800.040.5920943
TCAACGC134250.040.1093034
TATCAAC135450.039.5340842
CAACGCA137100.039.1452265
AACGCAG140900.038.1739586
GTACATG130000.032.9726451
ACGCAGA168250.031.827117
CGCAGAG168650.031.6810638
TACATGG136700.030.5450252
ACATGGG140450.028.414763
GCAGAGT182600.028.3152719
GTGGTAT26900.027.9245761
TGGTATC27750.027.4750182
GAGTACT106000.026.5490512-13
AGTACTT111600.024.60374512-13
GTACTTT115350.024.39704714-15
CAGAGTA179200.024.2697410-11
AGAGTAC175350.023.78471610-11
Produced by FastQC (version 0.11.5)