##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364576_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2709153 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.01363931826663 31.0 30.0 34.0 26.0 34.0 2 29.863593159928584 31.0 30.0 34.0 26.0 34.0 3 30.133596736692244 31.0 30.0 34.0 26.0 34.0 4 33.70014244304401 35.0 35.0 37.0 28.0 37.0 5 33.12573450078309 35.0 33.0 37.0 26.0 37.0 6 33.166276323264135 35.0 33.0 37.0 26.0 37.0 7 32.94502008561347 35.0 33.0 37.0 26.0 37.0 8 32.86455139299995 35.0 33.0 37.0 25.0 37.0 9 34.0772414847002 38.0 34.0 39.0 25.0 39.0 10-11 34.01769852053391 38.0 34.0 39.0 25.0 39.0 12-13 33.89512533991251 38.0 33.5 39.0 24.0 39.0 14-15 34.86225916365743 39.0 34.0 41.0 24.5 41.0 16-17 34.87653945716613 39.0 34.0 41.0 24.5 41.0 18-19 34.886544613759355 39.0 34.0 41.0 24.0 41.0 20-21 34.90239975372376 39.0 34.0 41.0 24.0 41.0 22-23 34.86786700492737 39.0 34.0 41.0 24.0 41.0 24-25 34.82551188507995 39.0 34.0 41.0 24.0 41.0 26-27 34.7097832791282 39.0 34.0 41.0 24.0 41.0 28-29 34.55371881912908 39.0 33.0 41.0 23.0 41.0 30-31 34.386073986961975 38.0 33.0 40.5 23.0 41.0 32-33 34.19111895858226 38.0 33.0 40.0 22.0 41.0 34-35 34.0097897756236 38.0 33.0 40.0 20.5 41.0 36-37 33.80619625395834 38.0 33.0 40.0 18.5 41.0 38-39 33.642455778614206 38.0 33.0 40.0 17.0 41.0 40-41 33.666056881984886 38.0 33.0 40.0 16.5 41.0 42-43 33.52471805763646 38.0 33.0 40.0 15.5 41.0 44-45 33.35340916515236 38.0 32.0 40.0 14.0 41.0 46-47 33.09490198597126 38.0 32.0 40.0 9.0 41.0 48-49 32.7937168923276 38.0 31.5 40.0 7.5 41.0 50-51 32.60219891604498 38.0 31.0 40.0 3.5 41.0 52-53 32.4069938095043 38.0 31.0 40.0 2.0 41.0 54-55 32.202769278811495 37.0 31.0 40.0 2.0 41.0 56-57 31.977086565432074 37.0 31.0 40.0 2.0 41.0 58-59 31.75720031316061 37.0 30.0 40.0 2.0 41.0 60-61 31.505350011608794 36.0 30.0 40.0 2.0 41.0 62-63 31.31827124566239 36.0 30.0 39.5 2.0 41.0 64-65 31.17682943709713 35.5 30.0 39.0 2.0 41.0 66-67 30.871887265134156 35.0 30.0 39.0 2.0 41.0 68-69 30.578521035910484 35.0 29.5 39.0 2.0 40.5 70-71 30.255982773951857 35.0 29.0 37.5 2.0 40.0 72-73 29.958712372464753 35.0 29.0 37.0 2.0 39.5 74-75 29.60917452797978 35.0 29.0 37.0 2.0 39.0 76-77 29.30791505684618 34.5 29.0 36.0 2.0 39.0 78-79 29.028767662808264 34.0 29.0 36.0 2.0 38.0 80-81 28.75902265394387 34.0 29.0 35.0 2.0 37.0 82-83 28.49147777921734 34.0 28.5 35.0 2.0 37.0 84-85 28.28479233177307 34.0 28.0 35.0 2.0 36.0 86-87 28.0908012578101 34.0 28.0 35.0 2.0 36.0 88-89 27.932150011461147 34.0 27.5 35.0 2.0 36.0 90-91 27.805956880250026 34.0 27.0 35.0 2.0 35.0 92-93 27.700502149564826 34.0 27.0 35.0 2.0 35.0 94-95 27.598951406583534 34.0 27.0 35.0 2.0 35.0 96-97 27.493311562691368 34.0 27.0 35.0 2.0 35.0 98-99 27.415305078746016 34.0 27.0 35.0 2.0 35.0 100-101 27.3160670512149 34.0 27.0 35.0 2.0 35.0 102-103 26.04266019674784 31.5 24.5 34.0 2.0 35.0 104-105 26.9841072098918 33.5 25.5 35.0 2.0 35.0 106-107 27.149925456406486 34.0 26.0 35.0 2.0 35.0 108-109 27.15941993678467 34.0 26.0 35.0 2.0 35.0 110-111 27.08993308978858 34.0 26.0 35.0 2.0 35.0 112-113 27.00918884979918 34.0 25.5 35.0 2.0 35.0 114-115 26.904724650102818 34.0 25.0 35.0 2.0 35.0 116-117 26.785082090232628 34.0 25.0 35.0 2.0 35.0 118-119 26.644197282324033 34.0 25.0 35.0 2.0 35.0 120-121 26.450665023348627 34.0 25.0 35.0 2.0 35.0 122-123 26.157008481986807 34.0 24.5 35.0 2.0 35.0 124-125 24.328848167674543 31.5 13.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7526236826146437 1101 2 0.8312570980828795 1101 3 0.7096311121045105 1101 4 0.6731891902807234 1101 5 0.7467042376773776 1101 6 0.7266366147434482 1101 7 0.915156725193178 1101 8 0.9250178556015811 1101 9 1.0793628297703464 1101 10-11 1.0524799323882092 1101 12-13 0.9751890154700575 1101 14-15 1.0749343816768402 1101 16-17 1.0854435346592055 1101 18-19 1.196841921416599 1101 20-21 1.1043717383493998 1101 22-23 1.082677978254324 1101 24-25 1.1285596467884744 1101 26-27 1.072220375772197 1101 28-29 1.2529330985651157 1101 30-31 1.1754769821606814 1101 32-33 1.3163391883869195 1101 34-35 1.3296583161815434 1101 36-37 1.409346501164336 1101 38-39 1.276934239499674 1101 40-41 1.0773107936864363 1101 42-43 1.0508318271461548 1101 44-45 1.0786410811964373 1101 46-47 1.2317638474487467 1101 48-49 1.261499323960038 1101 50-51 1.2043608530251717 1101 52-53 1.3526199318740666 1101 54-55 1.3545604603898056 1101 56-57 1.3374281093782052 1101 58-59 1.3792259476999185 1101 60-61 1.2076596115797358 1101 62-63 1.2264123594903182 1101 64-65 1.1091242954977503 1101 66-67 1.272532179945216 1101 68-69 1.3197250625888621 1101 70-71 1.314166567133075 1101 72-73 1.2251233195417832 1101 74-75 1.2334337339038655 1101 76-77 1.3076722866370822 1101 78-79 1.2222484828032947 1101 80-81 1.1253284081026322 1101 82-83 1.2631161913804654 1101 84-85 1.2790482199749427 1101 86-87 1.30208198705175 1101 88-89 1.3411529179602333 1101 90-91 1.27287664435962 1101 92-93 1.2198346040292805 1101 94-95 1.1937692968729046 1101 96-97 1.237275170510717 1101 98-99 1.3594272297455703 1101 100-101 1.2965563183355044 1101 102-103 1.2588193641580858 1101 104-105 1.2657681897743132 1101 106-107 1.2200119628132313 1101 108-109 1.2647044188094156 1101 110-111 1.1529028892923243 1101 112-113 1.2950251117892435 1101 114-115 1.2746287418473692 1101 116-117 1.2169098643396907 1101 118-119 1.2680601423366973 1101 120-121 1.3197971024333413 1101 122-123 1.2930853904696882 1101 124-125 1.6041411216652826 1102 1 0.41340763839042793 1102 2 -0.17682668975744775 1102 3 0.3483418711014963 1102 4 0.43026668666296786 1102 5 0.6156922780643157 1102 6 0.5011527790462438 1102 7 0.5124036455885701 1102 8 0.6176856680210463 1102 9 0.8930772677344763 1102 10-11 0.9617944832305625 1102 12-13 1.0126396282150623 1102 14-15 1.179024544615686 1102 16-17 1.152641596916304 1102 18-19 1.2116079742594152 1102 20-21 1.09072125501676 1102 22-23 1.2204673590217254 1102 24-25 1.2380892868994238 1102 26-27 1.2201452201100693 1102 28-29 0.8539398991389078 1102 30-31 1.1790157000472945 1102 32-33 1.3064670103729128 1102 34-35 1.3534304442294811 1102 36-37 0.9314802463480376 1102 38-39 0.4779361549869492 1102 40-41 0.10739098668493341 1102 42-43 0.41437220814410836 1102 44-45 0.025735169902333155 1102 46-47 0.25538184677429143 1102 48-49 0.07741156598685706 1102 50-51 0.03865799089783195 1102 52-53 -0.22006148848119977 1102 54-55 -0.04594487332463615 1102 56-57 -0.06210396479074021 1102 58-59 0.10487997350820777 1102 60-61 0.026244468684289757 1102 62-63 -0.039696849709937254 1102 64-65 -0.039005892179272195 1102 66-67 -0.05408840205162946 1102 68-69 0.10952269878883314 1102 70-71 -0.4060472541949487 1102 72-73 -0.18307960137630275 1102 74-75 -0.05380517155553122 1102 76-77 -0.3810824866258109 1102 78-79 -0.31314275686681015 1102 80-81 -0.3407101188655943 1102 82-83 -0.569667539414656 1102 84-85 -0.4360817172656368 1102 86-87 -0.15500598256809894 1102 88-89 -0.3596098343581211 1102 90-91 -0.3491277854319854 1102 92-93 -0.17341504280199516 1102 94-95 0.024674572499669978 1102 96-97 -0.26143810012380087 1102 98-99 -0.3667422757260894 1102 100-101 -0.17625672055439168 1102 102-103 -0.32049340962797856 1102 104-105 -0.17211799571012065 1102 106-107 -0.038805194095502316 1102 108-109 0.08190187497637424 1102 110-111 0.042041305491512304 1102 112-113 0.22203325206468705 1102 114-115 0.11058686974645227 1102 116-117 0.04641676879734291 1102 118-119 0.29302366177121897 1102 120-121 0.5077767470172319 1102 122-123 0.4077996468492131 1102 124-125 0.7028987217325842 1103 1 0.1939433016158354 1103 2 -0.1550818889270964 1103 3 0.14740073736089343 1103 4 0.08258979530306476 1103 5 0.17034071000843198 1103 6 0.20733946734115705 1103 7 0.26426248684687437 1103 8 0.29954427304853226 1103 9 0.5534575698244808 1103 10-11 0.5082306337312517 1103 12-13 0.518750993328986 1103 14-15 0.5172015120123916 1103 16-17 0.4717233628007307 1103 18-19 0.5662547205982023 1103 20-21 0.5521494376487723 1103 22-23 0.6529977454412119 1103 24-25 0.5058980147423284 1103 26-27 0.533784018758233 1103 28-29 0.6530963336481008 1103 30-31 0.7003905289645047 1103 32-33 0.7245053742579444 1103 34-35 0.6202283625438128 1103 36-37 0.6851596643934101 1103 38-39 0.7185121447817835 1103 40-41 0.5817040840597656 1103 42-43 0.6527087420769462 1103 44-45 0.6865705854298838 1103 46-47 0.7270879771116938 1103 48-49 0.6547615950973906 1103 50-51 0.6171296242518451 1103 52-53 0.5745625747774739 1103 54-55 0.5999357164256054 1103 56-57 0.6103774455663107 1103 58-59 0.6275307564411143 1103 60-61 0.6796264327565353 1103 62-63 0.643185606755921 1103 64-65 0.5296385390974905 1103 66-67 0.5299560639707508 1103 68-69 0.5454212814531338 1103 70-71 0.5562555976524024 1103 72-73 0.5341057846962123 1103 74-75 0.5664007577396006 1103 76-77 0.6173911792975453 1103 78-79 0.5333092407846607 1103 80-81 0.5646783922453764 1103 82-83 0.5866728465569757 1103 84-85 0.6267761662735545 1103 86-87 0.6946322397752986 1103 88-89 0.6895261760281635 1103 90-91 0.6610357994888076 1103 92-93 0.5992674221288006 1103 94-95 0.5421826597141184 1103 96-97 0.5383436078348645 1103 98-99 0.5697001914882733 1103 100-101 0.5727516021526569 1103 102-103 0.3190118393716084 1103 104-105 0.3259235031547405 1103 106-107 0.48070470323759906 1103 108-109 0.4991019885640249 1103 110-111 0.48979596846641726 1103 112-113 0.5194626178193893 1103 114-115 0.563030591444889 1103 116-117 0.5778380515208461 1103 118-119 0.5002021503959675 1103 120-121 0.5410912598083897 1103 122-123 0.5398281904222308 1103 124-125 0.8985271227796758 1104 1 0.42382210491205186 1104 2 0.6363205004747243 1104 3 0.34735308284919597 1104 4 0.11091280541064918 1104 5 0.42831167528855474 1104 6 0.42081441361472116 1104 7 0.587767844526212 1104 8 0.4972016048793293 1104 9 0.5908677427965472 1104 10-11 0.634048415079917 1104 12-13 0.564383986826563 1104 14-15 0.6985432742854414 1104 16-17 0.6469344987622776 1104 18-19 0.612462532254682 1104 20-21 0.535669379450411 1104 22-23 0.6227889288961137 1104 24-25 0.8030512907597966 1104 26-27 0.6507214278952418 1104 28-29 0.8052202399828161 1104 30-31 0.6693998446157892 1104 32-33 0.900655212013902 1104 34-35 0.9830222464210507 1104 36-37 1.0189327851768866 1104 38-39 1.1235699690425278 1104 40-41 1.0684036407807227 1104 42-43 1.026162617919674 1104 44-45 0.9711380631824795 1104 46-47 0.932513828878534 1104 48-49 0.953204449395443 1104 50-51 0.7847272901016424 1104 52-53 0.9693790243321772 1104 54-55 0.7036023224689814 1104 56-57 0.8466774928754965 1104 58-59 0.960059248429058 1104 60-61 0.9620175727008018 1104 62-63 0.9100889440681357 1104 64-65 0.9814893240732019 1104 66-67 1.0231346486777966 1104 68-69 0.775049582017278 1104 70-71 0.39648435081929634 1104 72-73 0.4247479177305742 1104 74-75 0.8408365294721882 1104 76-77 0.9382816107550518 1104 78-79 0.945006207586502 1104 80-81 0.8872426596493455 1104 82-83 0.9429432133931179 1104 84-85 0.9486430547907752 1104 86-87 0.9238774913024876 1104 88-89 0.8738491654163987 1104 90-91 0.9269899889995195 1104 92-93 0.9431978028008672 1104 94-95 0.9495608500628165 1104 96-97 0.903564677575055 1104 98-99 0.9516798220622107 1104 100-101 0.8634117207364191 1104 102-103 0.7721795406630001 1104 104-105 0.8559347376483188 1104 106-107 0.8552790931965397 1104 108-109 0.8764057791696374 1104 110-111 0.8964379886954141 1104 112-113 0.8927596308944494 1104 114-115 0.9264125596222677 1104 116-117 0.9495307535892152 1104 118-119 0.9319325731973898 1104 120-121 0.803108973704056 1104 122-123 0.9792560254915799 1104 124-125 1.4086713179651902 1105 1 0.21319204517421397 1105 2 -0.0426795493661416 1105 3 0.16596650997828988 1105 4 0.19403996369811694 1105 5 -0.007787351390547315 1105 6 0.22657487841639323 1105 7 0.3831095748586506 1105 8 0.4911230129549722 1105 9 0.5633544459657145 1105 10-11 0.5132548061028643 1105 12-13 0.5463652324209889 1105 14-15 0.6623653566367977 1105 16-17 0.532600408322466 1105 18-19 0.7025357983265863 1105 20-21 0.7127979760028467 1105 22-23 0.666920451819351 1105 24-25 0.5460662358992394 1105 26-27 0.536981582971535 1105 28-29 0.6161126050742709 1105 30-31 0.7732784607485641 1105 32-33 0.8215557652475098 1105 34-35 0.8406978108688534 1105 36-37 0.8672257355425046 1105 38-39 0.8147684994490163 1105 40-41 0.648832557026708 1105 42-43 0.6649950600508774 1105 44-45 0.6686346277228381 1105 46-47 0.6460497534621794 1105 48-49 0.6624621765095853 1105 50-51 0.42643213249770184 1105 52-53 0.5780642935084543 1105 54-55 0.5815893211232748 1105 56-57 0.47987820203981 1105 58-59 0.23677245697036042 1105 60-61 0.2733334427443026 1105 62-63 0.4866771232389304 1105 64-65 0.29655806886660585 1105 66-67 0.539709442016207 1105 68-69 0.5390140668407319 1105 70-71 0.6074538980506397 1105 72-73 0.5899353549844868 1105 74-75 0.5724006070245764 1105 76-77 0.6089236323005451 1105 78-79 0.56748030813689 1105 80-81 0.5557880895980105 1105 82-83 0.44236975863794115 1105 84-85 0.45760501197035097 1105 86-87 0.5819654533095076 1105 88-89 0.5220398708070455 1105 90-91 0.3497980233469953 1105 92-93 0.481443689149458 1105 94-95 0.46355773144867385 1105 96-97 0.44763553704517633 1105 98-99 0.4183053818819289 1105 100-101 0.46239382786579597 1105 102-103 0.22725896564181625 1105 104-105 0.371857327664479 1105 106-107 0.3264209474191304 1105 108-109 0.4259699564037547 1105 110-111 0.3104045383547138 1105 112-113 0.2998373185631067 1105 114-115 0.3500502307722364 1105 116-117 0.12111790034207814 1105 118-119 0.16578278003698088 1105 120-121 0.18482264505339074 1105 122-123 0.25533682465425045 1105 124-125 0.7574467279063057 1106 1 -0.008770873907408827 1106 2 -0.4825345792189282 1106 3 0.011092398211548726 1106 4 -0.16886287428172864 1106 5 0.2709066946447507 1106 6 0.4414151638154493 1106 7 0.46895571437350014 1106 8 0.4136461921677679 1106 9 0.5483544605209474 1106 10-11 0.49385224030592667 1106 12-13 0.5943566390098525 1106 14-15 0.5390805019417115 1106 16-17 0.5864144913862859 1106 18-19 0.5846800465341033 1106 20-21 0.5487959557361393 1106 22-23 0.5608716452031217 1106 24-25 0.5911930265571073 1106 26-27 0.7226213583551342 1106 28-29 0.8082123142606648 1106 30-31 0.6712640743536369 1106 32-33 0.6339815990441835 1106 34-35 0.6983632675102527 1106 36-37 0.7593857034045399 1106 38-39 0.7513175112940687 1106 40-41 0.7605844543016786 1106 42-43 0.7605971955950466 1106 44-45 0.6689980719573612 1106 46-47 0.603901255125848 1106 48-49 0.7899143989485182 1106 50-51 0.7567149116161787 1106 52-53 0.6699563285923915 1106 54-55 0.6501767063916333 1106 56-57 0.20622440123112185 1106 58-59 0.6781095300289088 1106 60-61 0.8156165187895823 1106 62-63 0.6062505383920929 1106 64-65 0.6858888773517258 1106 66-67 0.5390573383479982 1106 68-69 0.6512058524479336 1106 70-71 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2216 62-63 -1.9206774171731453 2216 64-65 -1.7872702447563142 2216 66-67 -1.6254668450310632 2216 68-69 -1.6206231698420872 2216 70-71 -1.6108637330833098 2216 72-73 -1.4637244113516736 2216 74-75 -1.409572647278182 2216 76-77 -1.5168597164853175 2216 78-79 -1.5499935204061615 2216 80-81 -1.5168149126463177 2216 82-83 -1.4098518562796194 2216 84-85 -1.3791351115689139 2216 86-87 -1.5778128400024478 2216 88-89 -1.5540564558094978 2216 90-91 -1.50525298349665 2216 92-93 -1.6216355344182816 2216 94-95 -1.5436731617345316 2216 96-97 -1.603339645787269 2216 98-99 -1.6062010076378819 2216 100-101 -1.6745573947734407 2216 102-103 -1.5699730476810956 2216 104-105 -1.5308177279038944 2216 106-107 -1.5651626467178161 2216 108-109 -1.5846179010119066 2216 110-111 -1.5061763903895553 2216 112-113 -1.5004223109270676 2216 114-115 -1.6392057130534319 2216 116-117 -1.6294438564688392 2216 118-119 -1.7037920932050419 2216 120-121 -1.8188342467073753 2216 122-123 -1.7684027685056094 2216 124-125 -2.15974474445661 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87350.0 3 29117.0 4 13948.0 5 11159.0 6 12088.0 7 14246.0 8 15728.0 9 17433.0 10 18912.0 11 20814.0 12 23295.0 13 26320.0 14 30785.0 15 26993.0 16 18187.0 17 15451.0 18 14449.0 19 13367.0 20 13217.0 21 13723.0 22 15793.0 23 19833.0 24 25952.0 25 34880.0 26 42850.0 27 47730.0 28 55478.0 29 65735.0 30 75479.0 31 89022.0 32 108386.0 33 134093.0 34 172370.0 35 238981.0 36 389202.0 37 575760.0 38 180534.0 39 493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00105891733277 19.309575553714744 13.686783632818015 24.002581896134473 2 15.718971944367851 20.68377090551918 38.56648184875494 25.030775301358027 3 19.520529110020735 25.067687207034815 29.03161984575991 26.38016383718454 4 13.09671381346686 16.94066641517437 35.81840236378127 34.14421740757751 5 13.666374693492763 38.40573049953251 33.3573998958346 14.570494911140125 6 32.45719234018898 36.55965536091907 16.841167700753704 14.141984598138238 7 28.695244208679128 32.70547344742547 20.152139255257378 18.447143088638022 8 26.742303014195194 35.47920554149756 18.901113504354903 18.877377939952346 9 26.85066892181461 15.901303071433237 19.29114922107697 37.95687878567519 10-11 25.61533860302823 26.768472708205948 26.504772642668513 21.11141604609731 12-13 26.29482351125979 23.852768743588864 27.410799611539105 22.441608133612238 14-15 23.17031307213475 24.87547764023576 25.51811415527811 26.43609513235138 16-17 22.951873781673715 28.568937012277296 25.816428565891776 22.662760640157213 18-19 22.668587597336167 26.492214121756515 28.69214852810164 22.14704975280568 20-21 23.355079021062007 25.73537269212377 28.840809716957853 22.068738569856368 22-23 23.103586250019838 25.581094903093327 28.679332617980602 22.63598622890623 24-25 22.957710398785157 25.90516297898273 28.689317288466178 22.447809333765942 26-27 22.705196052050216 26.279818821602174 28.580316431002608 22.434668695345003 28-29 22.488504709774602 26.08859669424355 28.877549551464977 22.545349044516865 30-31 22.601528964956945 25.8118496814318 28.878398525295545 22.708222828315712 32-33 22.532965100162304 26.000617167062916 28.706075293643433 22.760342439131346 34-35 23.071897378996315 25.950324695578285 28.389038935785464 22.588738989639936 36-37 22.93886687093715 25.752458425197837 28.367113263813447 22.941561440051558 38-39 22.736312788535752 26.077154003483745 28.563503057966827 22.623030150013676 40-41 22.927830211139792 25.676106148305394 28.71777636774298 22.678287272811833 42-43 22.879567894467385 25.87581801396968 28.271253044770823 22.973361046792114 44-45 23.0417034401527 26.111574355527356 27.850162024809965 22.996560179509977 46-47 22.9620106357965 26.342384501724343 27.451864106604535 23.243740755874622 48-49 23.37643905678269 26.46319716900448 27.090681109557117 23.069682664655705 50-51 23.08170174990149 26.47984932557322 27.03937855104133 23.39907037348396 52-53 23.596110517239342 26.43812528791297 26.784802033994403 23.180962160853287 54-55 23.226890470933167 26.3416462636108 26.98169870804639 23.44976455740964 56-57 23.144131320566384 26.882373178923093 26.943614076768547 23.029881423741976 58-59 23.340502906094716 26.41785160866904 26.91382190766496 23.327823577571284 60-61 23.37695582346217 26.410726895084924 27.26931627707996 22.943001004372952 62-63 23.248742319093825 26.4319143289434 27.166848826921182 23.15249452504159 64-65 23.00694369210706 26.222011742061486 27.146526981991947 23.624517583839506 66-67 23.02108814083221 26.85394660249901 26.768255613470338 23.356709643198446 68-69 22.942359280254617 26.452663702024896 27.190754304159697 23.41422271356079 70-71 22.844114009064825 26.533883468375542 27.12993692124439 23.492065601315247 72-73 23.139224694950784 26.376288087088472 27.011560439738915 23.47292677822183 74-75 22.94322358789835 26.583578123950304 26.78259123449034 23.690607053661004 76-77 23.12292808859448 26.513508096441953 26.91173219083603 23.451831624127543 78-79 23.02760309218416 26.545824469861984 26.890249461732136 23.53632297622172 80-81 22.750013841979957 26.87250613661111 26.919919531956516 23.457560489452412 82-83 23.001451007012154 26.742066616392652 26.691257378228546 23.56522499836665 84-85 22.968451763337104 26.50431703192843 26.63847335311073 23.888757851623737 86-87 23.055748420262717 26.34421164105534 26.965845044558208 23.634194894123738 88-89 22.870161319941776 26.48605940048049 26.76548598169996 23.87829329787778 90-91 22.83911666102222 26.69259113320494 26.98434288951988 23.48394931625296 92-93 22.883153709604965 26.395570614328285 26.962495510483414 23.75878016558334 94-95 22.851002296843756 26.303668767262085 26.84898690642184 23.996342029472316 96-97 22.998622712072255 26.324398373242445 26.804824900597907 23.872154014087393 98-99 22.704093733037215 26.170394072768165 26.787066562497518 24.3384456316971 100-101 22.486324310087998 26.23502936681265 27.06076622252704 24.217880100572312 102-103 23.07935358394303 26.0275259463013 26.85315299652696 24.039967473228717 104-105 22.824015476423813 26.061872474533555 26.9981429620254 24.115969087017234 106-107 22.620141592861557 26.22884195539819 27.115869057148203 24.03514739459205 108-109 22.70762216368812 26.240461960052446 26.61980575471587 24.43211012154357 110-111 22.34801016556884 26.283698362398468 27.073430916396134 24.294860555636557 112-113 22.582519333533398 26.137966368086264 27.051960520502167 24.227553777878178 114-115 22.154654978880853 26.302464275734888 26.86219641341777 24.680684331966486 116-117 22.12407346502763 26.179824469123748 27.064067625564153 24.63203444028447 118-119 22.253911093245748 26.22478317023808 26.646151029491506 24.87515470702467 120-121 21.977680641150954 26.220411143364213 26.96683213196276 24.83507608352208 122-123 22.13203407273753 26.279019633610286 26.796013182588 24.79293311106419 124-125 21.94094985898926 26.098587084938192 26.783691666364966 25.176771389707582 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 288.0 1 215.0 2 229.5 3 364.5 4 309.5 5 257.5 6 511.5 7 1174.0 8 1984.5 9 2339.5 10 1963.0 11 1436.5 12 1332.5 13 1463.0 14 1672.5 15 1945.0 16 2310.5 17 2747.0 18 3157.0 19 3606.5 20 4122.5 21 4751.0 22 5639.5 23 6551.0 24 7749.5 25 9674.0 26 12501.0 27 15832.0 28 18635.5 29 21644.5 30 25935.5 31 30979.5 32 36104.0 33 41024.0 34 46834.0 35 51348.5 36 55618.5 37 62292.5 38 67214.0 39 68358.0 40 68359.5 41 72713.5 42 79041.0 43 84644.5 44 88944.5 45 90808.0 46 91982.0 47 95581.0 48 105439.5 49 111097.0 50 111920.5 51 111657.0 52 107060.0 53 105270.0 54 104396.5 55 97969.0 56 90095.5 57 81743.5 58 73255.0 59 65475.5 60 55404.0 61 44091.5 62 35032.0 63 27716.0 64 21789.0 65 17470.5 66 12939.0 67 9973.0 68 7273.0 69 4507.5 70 3218.0 71 2513.5 72 2081.5 73 1656.5 74 1301.5 75 1109.0 76 729.0 77 355.5 78 192.5 79 115.0 80 79.0 81 55.5 82 40.5 83 26.0 84 16.0 85 8.5 86 7.0 87 9.5 88 7.5 89 3.0 90 3.0 91 2.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09652463334481294 2 0.0 3 0.0 4 0.09752125479808635 5 0.0 6 0.0 7 0.0020670667179003917 8 0.005093842983397394 9 0.0033958953222649295 10-11 5.352226323134942E-4 12-13 0.0 14-15 3.691190567679271E-5 16-17 2.2147143406075625E-4 18-19 0.0018271393310012392 20-21 0.0012365488401725557 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.8455952838396355E-5 52-53 3.691190567679271E-5 54-55 0.0 56-57 0.006607231116145895 58-59 7.013262078590615E-4 60-61 0.0 62-63 0.0 64-65 1.2919166986877448E-4 66-67 0.0 68-69 1.8455952838396355E-5 70-71 0.0 72-73 0.0 74-75 0.0012919166986877448 76-77 0.0 78-79 0.0 80-81 1.1073571703037812E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012365488401725557 90-91 1.8455952838396355E-5 92-93 0.0023623619633147334 94-95 1.8455952838396355E-5 96-97 0.007603852569419298 98-99 0.0035989108034872896 100-101 0.002122434576415581 102-103 0.0 104-105 0.0 106-107 0.0017533155196476535 108-109 3.691190567679271E-5 110-111 2.952952454143417E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.227976419198177E-4 122-123 0.004613988209599089 124-125 0.08367929016928907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2709153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.752077735061285 #Duplication Level Percentage of deduplicated Percentage of total 1 74.6916218068458 34.91988508871454 2 12.534541484436792 11.720317157074787 3 4.806304722831401 6.741141960606176 4 2.4533451105425192 4.587959252760656 5 1.3679909648688358 3.1978209965204654 6 0.883323794009601 2.4778333609659673 7 0.5788324894288894 1.8943135078950937 8 0.41745200256139675 1.5613398779525938 9 0.30406221746049106 1.2793986384307188 >10 1.7144574123874272 14.80945557141926 >50 0.14345610454761829 4.671654328358971 >100 0.09660713925287329 8.344655307988639 >500 0.005547847107343152 1.7342074668353782 >1k 0.0022983938016070283 1.5563884887035642 >5k 1.5850991735220883E-4 0.5036289957732322 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7361 0.27170853768687114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6231 0.2299980842720954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3949 0.1457651155176544 No Hit CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA 2844 0.10497745974479845 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.691190567679271E-5 0.0 12-13 0.0 0.0 0.0 3.691190567679271E-5 0.0 14-15 0.0 0.0 0.0 3.691190567679271E-5 0.0 16-17 0.0 0.0 0.0 3.691190567679271E-5 0.0 18-19 0.0 0.0 0.0 3.691190567679271E-5 0.0 20-21 0.0 0.0 0.0 9.227976419198178E-5 0.0 22-23 0.0 0.0 0.0 3.5066310392953075E-4 0.0 24-25 0.0 0.0 0.0 8.674297834046287E-4 0.0 26-27 0.0 0.0 0.0 0.0012180928873341593 0.0 28-29 0.0 0.0 0.0 0.0018640512366780318 0.0 30-31 0.0 0.0 0.0 0.003488175086456911 0.0 32-33 0.0 0.0 0.0 0.007050173984267407 0.0 34-35 0.0 0.0 0.0 0.011811809816573666 0.0 36-37 0.0 0.0 0.0 0.02120588981131741 0.0 38-39 0.0 0.0 0.0 0.03670889019557035 0.0 40-41 0.0 0.0 0.0 0.05507256326977472 0.0 42-43 0.0 0.0 0.0 0.07378689944790862 0.0 44-45 0.0 0.0 0.0 0.09600786666533784 0.0 46-47 0.0 0.0 0.0 0.12369179592293236 0.0 48-49 0.0 0.0 0.0 0.15456860502156947 0.0 50-51 0.0 0.0 0.0 0.19013322614115924 0.0 52-53 0.0 0.0 0.0 0.22638071751576969 0.0 54-55 0.0 0.0 0.0 0.2646214517969269 0.0 56-57 0.0 0.0 0.0 0.30891573860907817 0.0 58-59 0.0 0.0 0.0 0.36352690305789304 0.0 60-61 0.0 0.0 0.0 0.426387878425471 0.0 62-63 0.0 0.0 0.0 0.49133437646378775 0.0 64-65 0.0 0.0 0.0 0.556022491162367 0.0 66-67 0.0 0.0 0.0 0.6324485918661663 0.0 68-69 0.0 0.0 0.0 0.7203358392826097 0.0 70-71 0.0 0.0 0.0 0.8235415275549222 0.0 72-73 0.0 0.0 0.0 0.9312320123669648 0.0 74-75 0.0 0.0 0.0 1.0383134507353406 0.0 76-77 0.0 0.0 0.0 1.160012003751726 0.0 78-79 0.0 0.0 0.0 1.3021043846545397 0.0 80-81 0.0 0.0 0.0 1.4667868518315503 0.0 82-83 0.0 0.0 0.0 1.6460679777037326 0.0 84-85 0.0 0.0 0.0 1.8193509189034358 0.0 86-87 0.0 0.0 0.0 1.9964173304350106 0.0 88-89 1.8455952838396355E-5 0.0 0.0 2.202884074838151 0.0 90-91 3.691190567679271E-5 0.0 0.0 2.4481821440132765 0.0 92-93 3.691190567679271E-5 0.0 0.0 2.7075067373455837 0.0 94-95 3.691190567679271E-5 0.0 0.0 2.9696735474150042 0.0 96-97 3.691190567679271E-5 0.0 0.0 3.238078469543802 0.0 98-99 3.691190567679271E-5 0.0 0.0 3.532801580420154 0.0 100-101 3.691190567679271E-5 0.0 0.0 3.8706377971269985 0.0 102-103 3.691190567679271E-5 0.0 0.0 4.246825483832032 0.0 104-105 3.691190567679271E-5 0.0 0.0 4.6271842158785415 0.0 106-107 3.691190567679271E-5 0.0 0.0 5.005088306197546 0.0 108-109 3.691190567679271E-5 0.0 0.0 5.395985387314781 0.0 110-111 3.691190567679271E-5 0.0 0.0 5.844538865099166 0.0 112-113 3.691190567679271E-5 0.0 0.0 6.332920289108809 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5475 0.0 66.31342 1 GTATCAA 10010 0.0 53.661034 1 ATCAACG 13280 0.0 40.592094 3 TCAACGC 13425 0.0 40.109303 4 TATCAAC 13545 0.0 39.534084 2 CAACGCA 13710 0.0 39.145226 5 AACGCAG 14090 0.0 38.173958 6 GTACATG 13000 0.0 32.972645 1 ACGCAGA 16825 0.0 31.82711 7 CGCAGAG 16865 0.0 31.681063 8 TACATGG 13670 0.0 30.545025 2 ACATGGG 14045 0.0 28.41476 3 GCAGAGT 18260 0.0 28.315271 9 GTGGTAT 2690 0.0 27.924576 1 TGGTATC 2775 0.0 27.475018 2 GAGTACT 10600 0.0 26.54905 12-13 AGTACTT 11160 0.0 24.603745 12-13 GTACTTT 11535 0.0 24.397047 14-15 CAGAGTA 17920 0.0 24.26974 10-11 AGAGTAC 17535 0.0 23.784716 10-11 >>END_MODULE