FastQCFastQC Report
Thu 26 May 2016
SRR1364575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295225
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95250.043.038811
ATCAACG105750.038.146123
TCAACGC105700.038.107874
CAACGCA108900.036.878815
AACGCAG113500.035.484876
TATCAAC114750.035.1542632
GGTATCA60300.034.5347821
GTACATG137600.030.7437781
ACGCAGA130400.030.6122597
CGCAGAG128650.030.014838
TACATGG137500.029.4243742
ACATGGG142100.027.2994883
GCAGAGT155350.023.975589
CATGGGG81700.020.9735264
AGAGTAC139200.020.2202310-11
CATGGGA70950.019.371384
GAGTACA124650.018.9975171
AGTACAT110950.018.7690262
CAGAGTA148650.018.75464410-11
GAGTACT71150.018.4013612-13
Produced by FastQC (version 0.11.5)