##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364575_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4295225 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.138051906477543 31.0 30.0 33.0 24.0 34.0 2 29.13976846381738 31.0 30.0 34.0 23.0 34.0 3 29.29682170317038 31.0 30.0 34.0 24.0 34.0 4 32.740404751788326 35.0 33.0 37.0 26.0 37.0 5 32.81140266225867 35.0 33.0 37.0 26.0 37.0 6 32.95446804300124 35.0 33.0 37.0 26.0 37.0 7 32.82278367256663 35.0 33.0 37.0 26.0 37.0 8 32.7793538173204 35.0 33.0 37.0 25.0 37.0 9 34.02764465190997 38.0 34.0 39.0 25.0 39.0 10-11 33.939587332444745 38.0 34.0 39.0 24.0 39.0 12-13 33.88014236739635 38.0 34.0 39.0 23.5 39.0 14-15 34.688843308557765 39.0 33.5 41.0 21.0 41.0 16-17 34.792397371499746 39.0 34.0 41.0 23.0 41.0 18-19 34.5819817355319 39.0 33.5 41.0 19.0 41.0 20-21 34.29602512091916 39.0 33.0 41.0 18.0 41.0 22-23 34.204860164484984 39.0 33.0 41.0 18.0 41.0 24-25 34.022078587268425 39.0 33.0 41.0 18.0 41.0 26-27 33.93976182854216 38.0 32.5 41.0 17.5 41.0 28-29 34.00082661560221 38.5 33.0 41.0 17.5 41.0 30-31 33.95484054968017 38.0 33.0 40.0 17.5 41.0 32-33 33.9531859215757 38.0 33.0 40.0 17.5 41.0 34-35 33.47852336024306 38.0 32.0 40.0 15.5 41.0 36-37 33.35693031214896 38.0 32.0 40.0 14.5 41.0 38-39 33.16750100402191 38.0 32.0 40.0 13.0 41.0 40-41 33.40206112601785 38.0 32.0 40.0 15.0 41.0 42-43 33.29479875908713 38.0 32.0 40.0 14.0 41.0 44-45 33.16632248601645 38.0 32.0 40.0 11.0 41.0 46-47 33.17191916605067 38.0 32.0 40.0 12.5 41.0 48-49 33.04753208504793 38.0 32.0 40.0 9.0 41.0 50-51 32.89865711342246 38.0 32.0 40.0 8.5 41.0 52-53 32.84251162628267 38.0 32.0 40.0 8.0 41.0 54-55 32.88459044636777 38.0 32.0 40.0 7.5 41.0 56-57 32.75911506382087 38.0 32.0 40.0 7.0 41.0 58-59 32.56038321624595 37.0 31.5 40.0 3.5 41.0 60-61 32.349831149706944 37.0 31.0 40.0 2.0 41.0 62-63 32.07099069315345 36.5 31.0 40.0 2.0 41.0 64-65 31.788416089960364 36.0 31.0 39.5 2.0 41.0 66-67 31.54177965065858 36.0 31.0 39.0 2.0 41.0 68-69 31.504538179024383 35.0 31.0 39.0 2.0 41.0 70-71 31.264292091799614 35.0 31.0 39.0 2.0 40.5 72-73 30.971056347455605 35.0 30.5 38.0 2.0 40.0 74-75 30.65696546746678 35.0 30.0 37.0 2.0 39.5 76-77 30.336036878161217 35.0 30.0 37.0 2.0 39.0 78-79 30.010554976747436 35.0 30.0 36.0 2.0 39.0 80-81 29.70367140254585 35.0 30.0 36.0 2.0 38.0 82-83 29.437296067144327 34.0 30.0 35.5 2.0 37.0 84-85 29.181795482192435 34.0 29.0 35.0 2.0 37.0 86-87 28.97327648726202 34.0 29.0 35.0 2.0 36.0 88-89 28.767656059927013 34.0 29.0 35.0 2.0 36.0 90-91 28.624388128677776 34.0 29.0 35.0 2.0 36.0 92-93 28.49678526736085 34.0 29.0 35.0 2.0 35.5 94-95 28.370223445803187 34.0 29.0 35.0 2.0 35.0 96-97 28.256926237857158 34.0 29.0 35.0 2.0 35.0 98-99 28.162486482081846 34.0 29.0 35.0 2.0 35.0 100-101 28.068816651048547 34.0 29.0 35.0 2.0 35.0 102-103 26.85568485934963 32.5 26.0 34.0 2.0 35.0 104-105 27.764681012054083 34.0 27.5 35.0 2.0 35.0 106-107 27.918616836137804 34.0 29.0 35.0 2.0 35.0 108-109 27.876611353305123 34.0 29.0 35.0 2.0 35.0 110-111 27.7901285730084 34.0 28.5 35.0 2.0 35.0 112-113 27.699340313953286 34.0 28.0 35.0 2.0 35.0 114-115 27.579735403849625 34.0 28.0 35.0 2.0 35.0 116-117 27.47585912263036 34.0 27.5 35.0 2.0 35.0 118-119 27.33054938914725 34.0 27.0 35.0 2.0 35.0 120-121 27.157329127112085 34.0 27.0 35.0 2.0 35.0 122-123 26.921489561082364 34.0 27.0 35.0 2.0 35.0 124-125 25.432187254451165 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9640061416034307 1101 2 1.0147613647841638 1101 3 0.9714684981695036 1101 4 1.0974149773638402 1101 5 0.9842777291211817 1101 6 1.0323077786010586 1101 7 1.1138417264458624 1101 8 1.0550145857742947 1101 9 1.4119791605911232 1101 10-11 1.4427485677699394 1101 12-13 1.3034517736860636 1101 14-15 1.6146377884521996 1101 16-17 1.3769158754114486 1101 18-19 1.725877789711646 1101 20-21 2.0624835696566564 1101 22-23 1.9599487371814064 1101 24-25 2.0633346944525357 1101 26-27 2.15625308265588 1101 28-29 1.9866548095675043 1101 30-31 2.078585032016818 1101 32-33 2.0301162779469735 1101 34-35 2.342656102385348 1101 36-37 2.2096367455420136 1101 38-39 2.1580666803058506 1101 40-41 1.9523433037278934 1101 42-43 1.9472996203678221 1101 44-45 1.8893539076502393 1101 46-47 1.8228510384653092 1101 48-49 1.7897681551650848 1101 50-51 1.8237524722130232 1101 52-53 1.6431148130110884 1101 54-55 1.5401689153478912 1101 56-57 1.54911154552331 1101 58-59 1.5169580719224243 1101 60-61 1.5888689449102884 1101 62-63 1.5564419343996718 1101 64-65 1.5682118525871438 1101 66-67 1.499770946046521 1101 68-69 1.3963808896323293 1101 70-71 1.3170540823088395 1101 72-73 1.2672807203979168 1101 74-75 1.2454101478801896 1101 76-77 1.3981419698307143 1101 78-79 1.447965606462688 1101 80-81 1.4860110901603427 1101 82-83 1.368074476499416 1101 84-85 1.4617639665188484 1101 86-87 1.4659818353513465 1101 88-89 1.4017799863666376 1101 90-91 1.4119750650147225 1101 92-93 1.2864702195739284 1101 94-95 1.3348683693009207 1101 96-97 1.3511484954505164 1101 98-99 1.4680366326993415 1101 100-101 1.5529016884566467 1101 102-103 1.359694126800914 1101 104-105 1.448307162084415 1101 106-107 1.4459543422107934 1101 108-109 1.48537054859705 1101 110-111 1.3204548545980188 1101 112-113 1.3297719621083175 1101 114-115 1.5310366688965047 1101 116-117 1.5171469471319483 1101 118-119 1.5294785932032724 1101 120-121 1.5940692327838946 1101 122-123 1.691290918580279 1101 124-125 1.8763803825956167 1102 1 0.2106994259899686 1102 2 0.3998041952712441 1102 3 0.5583203807570172 1102 4 0.7183489494505366 1102 5 0.6875650404070441 1102 6 0.07871535479583258 1102 7 -0.4217951118181418 1102 8 0.40945445037793604 1102 9 0.7860453196644457 1102 10-11 0.7331925186822588 1102 12-13 0.8526715064285355 1102 14-15 1.1478499683390169 1102 16-17 0.984006790386168 1102 18-19 1.1194962052643262 1102 20-21 1.4730145912068693 1102 22-23 1.2614320098665104 1102 24-25 1.4232270280147645 1102 26-27 1.047793200337054 1102 28-29 1.4519663317665206 1102 30-31 1.29564929331503 1102 32-33 1.1613432958315641 1102 34-35 1.4676911294643773 1102 36-37 1.6354009365041264 1102 38-39 1.6876837764132873 1102 40-41 1.4221011749836734 1102 42-43 1.2562155105140747 1102 44-45 1.3409627016964052 1102 46-47 1.1165746517055553 1102 48-49 0.8441491448568854 1102 50-51 1.2722180961534804 1102 52-53 1.1094900919712813 1102 54-55 0.932976210394429 1102 56-57 0.9393204013004848 1102 58-59 1.0210846724730018 1102 60-61 0.9373927818335304 1102 62-63 0.8425808581079366 1102 64-65 0.9427236002342916 1102 66-67 0.9545770535433 1102 68-69 0.8465402184068473 1102 70-71 0.8146927852515056 1102 72-73 1.0143210885089182 1102 74-75 0.7368982918728015 1102 76-77 0.8784832040636239 1102 78-79 0.7483056786423745 1102 80-81 0.4221472558308861 1102 82-83 0.6215252352294627 1102 84-85 0.2719717950802014 1102 86-87 0.6482226833854305 1102 88-89 0.8014428928676871 1102 90-91 0.33176225727593334 1102 92-93 0.7033936615951966 1102 94-95 0.6410319952207892 1102 96-97 0.776864623008656 1102 98-99 0.8180597144105626 1102 100-101 0.7935889228116473 1102 102-103 0.6979651730190035 1102 104-105 0.9310186784486518 1102 106-107 0.8587426153776363 1102 108-109 0.4094552416838404 1102 110-111 0.6166198460530019 1102 112-113 0.41416689889041436 1102 114-115 0.5124420644027907 1102 116-117 0.38375516382889074 1102 118-119 0.7181445904226784 1102 120-121 1.0343353454241182 1102 122-123 0.8728691020851578 1102 124-125 1.1116625249510612 1103 1 0.5722184026333359 1103 2 0.6922525650796381 1103 3 0.5437038411978996 1103 4 0.6543536128226819 1103 5 0.7159840180447361 1103 6 0.7041608112395394 1103 7 0.7660950354410048 1103 8 0.8366963988521476 1103 9 1.0566837243735563 1103 10-11 1.0765858676596025 1103 12-13 1.0976063037359083 1103 14-15 1.4228103197325623 1103 16-17 1.212634451441538 1103 18-19 1.2912143198301607 1103 20-21 1.7124755193074108 1103 22-23 1.6894233045737366 1103 24-25 1.815203094674139 1103 26-27 1.7416421975628964 1103 28-29 1.691846739637576 1103 30-31 1.6898987538951147 1103 32-33 1.6255727953890897 1103 34-35 1.9980912945218918 1103 36-37 1.966435230684354 1103 38-39 2.0487171508452775 1103 40-41 1.7233087795615276 1103 42-43 1.6481459626324835 1103 44-45 1.3624307441738637 1103 46-47 1.39758098056776 1103 48-49 1.3966283635183316 1103 50-51 1.3032926603916835 1103 52-53 1.3152359083597034 1103 54-55 1.2175147453597717 1103 56-57 1.1243797126327806 1103 58-59 1.0421089985709813 1103 60-61 1.029941826786846 1103 62-63 1.0093492356720404 1103 64-65 1.2326353292615444 1103 66-67 1.214563033597198 1103 68-69 1.1794665838601084 1103 70-71 1.2301690408321413 1103 72-73 1.1353341041893863 1103 74-75 1.1900122611084676 1103 76-77 1.1801355193433771 1103 78-79 1.168042481498066 1103 80-81 1.0950637774922036 1103 82-83 1.165254789474723 1103 84-85 1.1910907508428004 1103 86-87 1.0872254774314278 1103 88-89 1.1038881802765843 1103 90-91 1.1967036916854568 1103 92-93 1.2278457899423358 1103 94-95 1.1146178201603831 1103 96-97 1.2777593888954435 1103 98-99 1.239198263296423 1103 100-101 1.273949314972409 1103 102-103 1.2140524397261068 1103 104-105 1.1540021925829436 1103 106-107 1.2181735149411317 1103 108-109 1.3201860935198546 1103 110-111 1.3291153716021178 1103 112-113 1.3766614301171103 1103 114-115 1.4752012096832416 1103 116-117 1.4844104223030037 1103 118-119 1.4534035119805822 1103 120-121 1.4438665472616243 1103 122-123 1.4349657800539575 1103 124-125 1.5664864801667768 1104 1 0.7732974021899572 1104 2 0.8436794973538042 1104 3 0.6919317125472055 1104 4 0.8585913702474386 1104 5 0.8378564266370319 1104 6 0.8118638894840515 1104 7 0.866660298565705 1104 8 0.8402342558982667 1104 9 1.1545907017648815 1104 10-11 1.0936117757074157 1104 12-13 1.0674992942505455 1104 14-15 1.1871407463605905 1104 16-17 1.2436269223296534 1104 18-19 1.5212303343819258 1104 20-21 1.7291399752578869 1104 22-23 1.4933502090207043 1104 24-25 1.8966577057334106 1104 26-27 1.9755903341610406 1104 28-29 1.8037003138554937 1104 30-31 1.7311064959778193 1104 32-33 1.7616636957102187 1104 34-35 2.0258101971656615 1104 36-37 2.1019323450040517 1104 38-39 2.0717842969436333 1104 40-41 1.7393567239665018 1104 42-43 1.7651293414478246 1104 44-45 1.612774618758536 1104 46-47 1.48476662600104 1104 48-49 1.5141262672028404 1104 50-51 1.2619524840414513 1104 52-53 1.4357480076248805 1104 54-55 1.2359866705009068 1104 56-57 1.203405890604401 1104 58-59 1.1814859429963604 1104 60-61 1.1883683657699677 1104 62-63 1.23530600426664 1104 64-65 1.2135593452454891 1104 66-67 1.165974614534452 1104 68-69 1.201671272371744 1104 70-71 1.1966509271821586 1104 72-73 1.1381131135782816 1104 74-75 1.1878359424239875 1104 76-77 1.1418959883986055 1104 78-79 1.1935083551514616 1104 80-81 1.19947093297759 1104 82-83 1.1052190500782757 1104 84-85 1.1101536096976936 1104 86-87 1.1651929233214418 1104 88-89 1.1770897085462337 1104 90-91 1.1948253095215762 1104 92-93 1.2074789254552165 1104 94-95 1.2292276550337995 1104 96-97 1.0992743339552824 1104 98-99 1.1636856863310356 1104 100-101 1.195711991651919 1104 102-103 1.2077831809869757 1104 104-105 1.2477155186564737 1104 106-107 1.2443114617763413 1104 108-109 1.331204573561262 1104 110-111 1.3759541788542577 1104 112-113 1.3519690365885992 1104 114-115 1.2935968419264299 1104 116-117 1.2919325773895345 1104 118-119 1.3087115033949672 1104 120-121 1.3346427822789977 1104 122-123 1.3721314594735148 1104 124-125 1.6275866562201777 1105 1 0.35360644876114833 1105 2 0.5596913831103336 1105 3 0.5736414478889671 1105 4 0.7459894058587793 1105 5 0.6446474818051797 1105 6 0.7097292463378295 1105 7 0.7816305593733475 1105 8 0.6616388136297431 1105 9 0.6144860561091647 1105 10-11 0.8813446619853167 1105 12-13 0.7586169150710589 1105 14-15 0.8843353776905047 1105 16-17 0.8360832844555759 1105 18-19 1.0879440755495438 1105 20-21 1.429602463973417 1105 22-23 1.5821000110717307 1105 24-25 1.6115750393028705 1105 26-27 1.419714620763962 1105 28-29 1.230890731765733 1105 30-31 1.2015357209988622 1105 32-33 1.3769280048949355 1105 34-35 1.8772960407369297 1105 36-37 1.8745383473680448 1105 38-39 1.8949356280609209 1105 40-41 1.505235033204869 1105 42-43 1.106908979874504 1105 44-45 1.2468354918797715 1105 46-47 1.2114920980565174 1105 48-49 1.2100079824495182 1105 50-51 1.1851902146037858 1105 52-53 1.1975978715498883 1105 54-55 0.9910729716603797 1105 56-57 1.0502468454477807 1105 58-59 0.9418994368150209 1105 60-61 0.9919214166458801 1105 62-63 1.0104117668873371 1105 64-65 1.0632065597589708 1105 66-67 0.9376598326788148 1105 68-69 0.723353045928409 1105 70-71 0.8731169942272494 1105 72-73 0.9568814135245276 1105 74-75 1.0017379355451013 1105 76-77 0.9916714793579864 1105 78-79 1.0142357069414167 1105 80-81 0.9304353605826456 1105 82-83 0.8635532300147162 1105 84-85 0.882550433732721 1105 86-87 0.9269874212401739 1105 88-89 1.0182613772441336 1105 90-91 0.9362849595294236 1105 92-93 0.927499346808208 1105 94-95 1.0281200639341002 1105 96-97 1.0637185648863223 1105 98-99 0.9295029804393025 1105 100-101 0.9629979660991381 1105 102-103 0.9081678449844404 1105 104-105 1.0004666649234046 1105 106-107 1.0321472493707553 1105 108-109 1.0381323696754734 1105 110-111 1.0309419785839076 1105 112-113 1.1062295782210825 1105 114-115 1.1235857485169234 1105 116-117 1.1515805092958225 1105 118-119 1.1729904078948294 1105 120-121 1.0463959175886473 1105 122-123 1.1496423710087882 1105 124-125 1.2796603577208394 1106 1 0.37804000438229224 1106 2 0.7245262104315096 1106 3 0.36097347726032325 1106 4 0.6488565365780943 1106 5 0.4934716686767615 1106 6 0.5370320676600784 1106 7 0.31038558032613395 1106 8 0.45773363229113073 1106 9 0.59124209735851 1106 10-11 0.8215741612908829 1106 12-13 0.8091819923124604 1106 14-15 1.069182007340828 1106 16-17 0.842313311139101 1106 18-19 0.890577088660585 1106 20-21 1.3078775073098043 1106 22-23 1.3384766772939258 1106 24-25 1.504528550636259 1106 26-27 1.6803807275909577 1106 28-29 1.439645318230994 1106 30-31 1.304177592411527 1106 32-33 1.2802489128839625 1106 34-35 1.549556765462107 1106 36-37 1.5070524532301874 1106 38-39 1.7137909444652166 1106 40-41 1.3852845460544572 1106 42-43 1.2749573293463001 1106 44-45 1.149004352458718 1106 46-47 0.9254562273659985 1106 48-49 0.9511296419521358 1106 50-51 0.9022127053259794 1106 52-53 0.9094505225979788 1106 54-55 0.7149247821998443 1106 56-57 0.7715044658345391 1106 58-59 0.7183790166506014 1106 60-61 0.7932222198485022 1106 62-63 0.6945761387607163 1106 64-65 0.7012326642187681 1106 66-67 0.5749692200064622 1106 68-69 0.7435889277058472 1106 70-71 0.8486825022105826 1106 72-73 0.7799035027203196 1106 74-75 0.7955993572482001 1106 76-77 0.712144400297742 1106 78-79 0.6942958572441356 1106 80-81 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104-105 -3.37889311727071 2216 106-107 -3.399144725684213 2216 108-109 -3.4596355784859263 2216 110-111 -3.4984608431701005 2216 112-113 -3.5491253409369534 2216 114-115 -3.6072968861124295 2216 116-117 -3.6966697508419166 2216 118-119 -3.7654726177272906 2216 120-121 -3.8431320052041826 2216 122-123 -3.8405885361566483 2216 124-125 -3.9097069412414776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 194149.0 3 34467.0 4 22193.0 5 17684.0 6 15993.0 7 17465.0 8 18980.0 9 20241.0 10 21708.0 11 23309.0 12 24976.0 13 26915.0 14 29434.0 15 27105.0 16 22153.0 17 20367.0 18 19127.0 19 18590.0 20 18639.0 21 19899.0 22 23762.0 23 30421.0 24 40258.0 25 50887.0 26 63438.0 27 74681.0 28 86814.0 29 102791.0 30 119318.0 31 139141.0 32 171938.0 33 219939.0 34 291059.0 35 400014.0 36 629665.0 37 895967.0 38 340605.0 39 1133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92764214107175 18.74985010821314 13.229990465136044 24.09251728557907 2 14.894437856762185 19.959125067839675 41.32549703111929 23.82094004427885 3 19.35118547303669 24.83330267688962 28.93683453203909 26.878677318034605 4 12.194664802134433 16.588911854052387 38.70019658148912 32.51622676232407 5 13.568566874071811 38.022643966196114 34.85547380534032 13.553315354391762 6 31.633455097446213 37.08734654345908 17.99133449276982 13.287863866324889 7 27.979144804746227 32.183036399268076 21.59091993874665 18.24689885723905 8 25.227875140417556 36.060229673649225 20.327433370778014 18.384461815155202 9 26.034563403452427 16.544469876401617 20.09545249132679 37.32551422881916 10-11 24.473025278070416 26.598431979698383 28.386394193552146 20.542148548679055 12-13 25.407528251372902 23.80931205110194 28.27301383599594 22.510145861529214 14-15 22.34354577950421 25.549646748387662 25.9775048777154 26.12930259439273 16-17 22.410954993021203 28.645715256486344 26.52852061274613 22.414809137746317 18-19 22.250972580098015 27.727909934740687 28.16557970989185 21.855537775269447 20-21 22.9599596827285 27.013386819609664 28.187328162356877 21.83932533530496 22-23 22.56937548732393 27.00694808389294 27.82597823913115 22.597698189651982 24-25 22.397732318489666 26.997929813916095 28.107240808208473 22.49709705938577 26-27 22.267247250860105 27.300318428697 27.859565376846103 22.572868943596788 28-29 22.260673350550974 27.545226574644715 27.812466314196264 22.381633760608043 30-31 22.586534008853217 27.220027374992462 27.65850919208849 22.534929424065826 32-33 22.333838158470545 27.225515738308804 27.63657433089326 22.804071772327394 34-35 22.673624729575852 27.19343189036883 27.835720889101857 22.29722249095346 36-37 22.728803647851944 26.921779737666572 27.77192380136777 22.577492813113714 38-39 22.479125074937865 27.224243200298005 27.766054164799282 22.530577559964847 40-41 22.881571334742414 27.158382925401675 27.52932938436441 22.4307163554915 42-43 22.571086867968134 27.126825885355977 27.437289407114562 22.864797839561326 44-45 22.643452857880327 27.268168692816257 27.525864463713607 22.562513985589806 46-47 22.7759605818659 27.397330759224687 26.996662925175528 22.830045733733883 48-49 22.624259579604573 27.3848746928026 27.203959046323746 22.786906681269084 50-51 22.813116331200888 27.55562012566492 27.083695667105072 22.547567876029113 52-53 22.944898113602896 27.345261307614848 27.041493751782504 22.66834682699975 54-55 22.648402964164827 27.371663093740217 27.24465373738042 22.735280204714538 56-57 22.688810912838985 27.443120000204885 27.13094675709322 22.737122329862906 58-59 22.746238341819332 27.33570752652924 27.11387920530609 22.80417492634534 60-61 22.765397353171295 27.320718932160393 27.235458280079378 22.678425434588934 62-63 22.725759062254248 27.273262877272273 27.356968552794193 22.64400950767929 64-65 22.465034201755486 27.26436368365358 27.35691036360984 22.913691750981087 66-67 22.53774114573275 27.42616578968843 27.147735240229874 22.888357824348947 68-69 22.67265428160264 27.277990974334138 27.408509181682277 22.640845562380953 70-71 22.530362154995622 27.12405286152841 27.23857848992639 23.10700649354958 72-73 22.556006789188572 27.358639386816918 27.103037776532595 22.982316047461918 74-75 22.49699637353065 27.41623866226317 27.14859258229692 22.938172381909258 76-77 22.697616720442298 27.162150646588152 27.19585257855286 22.944380054416687 78-79 22.709929284661857 27.19769387931142 27.190336792754945 22.902040043271775 80-81 22.671989934590673 27.338941111115766 27.050217154128443 22.938851800165118 82-83 22.803439872656636 27.359662354757997 27.137249168191445 22.699648604393925 84-85 22.740135848529473 27.34853238188919 27.03109848727366 22.880233282307678 86-87 22.670126648030326 27.35566488906263 27.096969543867555 22.877238919039485 88-89 22.766660558204755 27.283949639884046 26.997822143166665 22.951567658744526 90-91 22.572219304976148 27.400989897272833 27.164937470673916 22.861853327077103 92-93 22.6668650341858 27.28769902525181 27.238817263934507 22.80661867662788 94-95 22.45247308773129 27.272492287701482 27.294859263287012 22.980175361280214 96-97 22.576638960245447 27.09501480014679 27.257499734547398 23.070846505060366 98-99 22.418465133182792 27.13845753397931 27.15674544554172 23.28633188729618 100-101 22.396262076103095 27.0992963161519 27.390048307322818 23.114393300422186 102-103 22.361447886897658 26.887834746724558 27.474602611039 23.276114755338778 104-105 22.26136958120604 27.028052692712528 27.32534031854873 23.3852374075327 106-107 22.267004530381776 26.99467524736201 27.340221916300973 23.398098305955244 108-109 22.216740682967714 26.90935864826639 27.328940858744303 23.54495981002159 110-111 22.168606760739994 27.03236674558818 27.327021552336788 23.472004941335033 112-113 22.329063087498326 27.013858412539506 27.457455662974585 23.199622836987587 114-115 22.229475755053578 27.155492436368295 27.295601510980216 23.319430297597915 116-117 21.96465842883667 27.085042110715968 27.507965240470522 23.442334219976836 118-119 21.96912850898381 27.15813490562194 27.389810778247934 23.48292580714631 120-121 21.923475711223013 27.21794867765352 27.409929329654364 23.448646281469106 122-123 21.971829182406044 27.100733954565825 27.42294059100513 23.504496272023 124-125 21.89983857401144 27.0891298738679 27.37447802026348 23.63655353185718 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 285.0 1 292.0 2 341.0 3 362.5 4 320.0 5 323.5 6 323.0 7 350.5 8 492.0 9 620.0 10 664.5 11 723.5 12 802.0 13 921.0 14 1067.0 15 1212.0 16 1454.0 17 1710.0 18 2079.5 19 2547.0 20 3057.0 21 3989.0 22 5302.0 23 7202.5 24 10039.5 25 13819.0 26 19615.5 27 27126.0 28 34503.5 29 42647.0 30 51448.5 31 61110.0 32 72342.0 33 81138.0 34 88322.5 35 96075.5 36 105288.5 37 114607.0 38 122309.5 39 129160.0 40 136113.0 41 143684.0 42 147856.0 43 151211.5 44 155492.0 45 159236.5 46 161784.0 47 164335.0 48 168496.0 49 167147.0 50 160655.0 51 155311.5 52 150620.5 53 146585.0 54 142048.0 55 131710.5 56 119533.0 57 108991.5 58 100515.5 59 89932.5 60 74594.0 61 60339.0 62 48094.5 63 36546.5 64 26572.0 65 19829.5 66 15003.0 67 11369.0 68 8670.0 69 6664.5 70 5089.0 71 3814.0 72 2839.0 73 2310.0 74 1829.0 75 1335.0 76 962.5 77 646.0 78 417.5 79 290.5 80 213.5 81 143.0 82 108.5 83 86.5 84 64.5 85 58.0 86 46.5 87 27.5 88 18.0 89 18.5 90 22.5 91 18.0 92 8.5 93 7.0 94 10.5 95 10.5 96 7.5 97 7.0 98 7.0 99 7.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010709567019189915 2 0.004004446798479707 3 0.0073337252414017885 4 0.007310443573968768 5 0.013293832104255306 6 8.148583601557543E-4 7 0.006425740211513949 8 0.0 9 0.0024212934130342415 10-11 0.0 12-13 0.0028869267616946723 14-15 0.016157477198516956 16-17 0.026692431711959213 18-19 0.00479602349120244 20-21 0.006786606056725783 22-23 0.0019556600643738107 24-25 0.010290497005395527 26-27 0.01629716720311509 28-29 4.1907001379438795E-4 30-31 0.0014900267157133794 32-33 0.007880844426077796 34-35 0.008183506102707076 36-37 0.009859786157884628 38-39 0.0 40-41 0.021873126553323748 42-43 0.008299914439872182 44-45 0.014574323813071492 46-47 0.003399123445221147 48-49 0.022315478234551156 50-51 0.003538813449819276 52-53 0.0 54-55 0.015354259672077715 56-57 0.00405101013334575 58-59 2.6773917547974783E-4 60-61 0.003736707622999959 62-63 0.023561047442217813 64-65 0.014795499653685198 66-67 0.001827610893492192 68-69 0.01874174228358235 70-71 0.002712314255947011 72-73 0.010604799515741318 74-75 1.1640833716510776E-5 76-77 0.00509868516783172 78-79 0.0010825975356355021 80-81 0.0027705184245295647 82-83 0.0015365900505794226 84-85 0.0 86-87 0.004924072662084058 88-89 0.014807140487401707 90-91 0.019556600643738105 92-93 0.0038880384613145993 94-95 0.022059379892787924 96-97 0.015668562182423504 98-99 8.84703362454819E-4 100-101 0.007624746084314559 102-103 0.0 104-105 0.0013503367111152502 106-107 4.6563334866043106E-5 108-109 0.0 110-111 6.868091892741358E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.1640833716510776E-5 122-123 0.0 124-125 0.02321182243072249 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4295225.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.98893403383422 #Duplication Level Percentage of deduplicated Percentage of total 1 68.71750465961767 32.97679798080088 2 14.404350804673532 13.824988811313702 3 6.222824313303111 8.958801164257283 4 3.3640426691466963 6.457472869467376 5 2.0020911231755445 4.803910941989813 6 1.2900828006628604 3.714581905151647 7 0.8399855945846219 2.82170093015247 8 0.617620394882407 2.371115551036998 9 0.4506697254479486 1.9464443753011005 >10 1.9762911136308898 15.704633474325963 >50 0.08012005785652458 2.6167021701538222 >100 0.03200855260618596 2.606524470425514 >500 0.001474402437059924 0.5053793570165978 >1k 9.337879749781974E-4 0.6909459986070051 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1640833716510776E-4 2 0.0 0.0 0.0 2.3281667433021553E-5 1.1640833716510776E-4 3 0.0 0.0 0.0 2.3281667433021553E-5 1.1640833716510776E-4 4 0.0 0.0 0.0 4.6563334866043106E-5 1.1640833716510776E-4 5 0.0 0.0 0.0 4.6563334866043106E-5 1.1640833716510776E-4 6 0.0 0.0 0.0 4.6563334866043106E-5 1.3969000459812932E-4 7 2.3281667433021553E-5 0.0 0.0 4.6563334866043106E-5 1.3969000459812932E-4 8 2.3281667433021553E-5 0.0 0.0 4.6563334866043106E-5 1.3969000459812932E-4 9 2.3281667433021553E-5 0.0 0.0 4.6563334866043106E-5 1.3969000459812932E-4 10-11 3.492250114953233E-5 0.0 0.0 9.312666973208621E-5 1.6297167203115087E-4 12-13 4.6563334866043106E-5 0.0 0.0 1.3969000459812932E-4 4.1907001379438795E-4 14-15 4.6563334866043106E-5 0.0 0.0 1.3969000459812932E-4 5.005558498099635E-4 16-17 4.6563334866043106E-5 0.0 0.0 1.513308383146401E-4 5.354783509594958E-4 18-19 4.6563334866043106E-5 0.0 0.0 1.978941731806832E-4 5.354783509594958E-4 20-21 4.6563334866043106E-5 0.0 0.0 2.2117584061370475E-4 5.936825195420497E-4 22-23 4.6563334866043106E-5 0.0 0.0 3.3758417777881254E-4 6.28605020691582E-4 24-25 4.6563334866043106E-5 1.1640833716510776E-5 0.0 5.121966835264743E-4 6.28605020691582E-4 26-27 4.6563334866043106E-5 2.3281667433021553E-5 0.0 6.169641869750712E-4 7.100908567071574E-4 28-29 4.6563334866043106E-5 2.3281667433021553E-5 0.0 0.001094238369352013 7.217316904236682E-4 30-31 4.6563334866043106E-5 2.3281667433021553E-5 0.0 0.002514420082766328 7.333725241401789E-4 32-33 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.006844810225308336 7.450133578566897E-4 34-35 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.011955136226856566 7.566541915732005E-4 36-37 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.01982433981921785 7.682950252897113E-4 38-39 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.03642416869896222 8.730625287383083E-4 40-41 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.05564318516492151 8.84703362454819E-4 42-43 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.07506009580406149 8.963441961713298E-4 44-45 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.09924974826697089 9.778300321869053E-4 46-47 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.12924817675441916 0.0010709567019189913 48-49 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.16228486284187674 0.00119900587280061 50-51 6.984500229906466E-5 2.3281667433021553E-5 0.0 0.1993958407301131 0.001303773376249207 52-53 8.148583601557544E-5 2.3281667433021553E-5 0.0 0.23566867859076068 0.0014201817134143148 54-55 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.27572478740927425 0.0014667450482803578 56-57 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.32192725643010556 0.0014783858819968686 58-59 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.3755914998632202 0.0014900267157133794 60-61 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.4348782659814096 0.0014900267157133794 62-63 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.4987398797501877 0.0015831533854454656 64-65 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.5633232252093895 0.0015947942191619764 66-67 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.6414681419483264 0.0016064350528784872 68-69 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.7301945765355715 0.0016297167203115088 70-71 9.312666973208621E-5 4.6563334866043106E-5 0.0 0.8310041965205548 0.0016413575540280196 72-73 1.1640833716510776E-4 4.6563334866043106E-5 0.0 0.9374363391906129 0.001769406724909638 74-75 1.3969000459812932E-4 4.6563334866043106E-5 0.0 1.0458474235924777 0.0018974558957912566 76-77 1.3969000459812932E-4 4.6563334866043106E-5 0.0 1.1659575458794358 0.0019090967295077674 78-79 1.3969000459812932E-4 4.6563334866043106E-5 0.0 1.3058105221495964 0.0019090967295077674 80-81 1.3969000459812932E-4 4.6563334866043106E-5 0.0 1.4590737388611772 0.001932378396940789 82-83 1.3969000459812932E-4 4.6563334866043106E-5 0.0 1.6324173937337392 0.002025505066672875 84-85 1.3969000459812932E-4 4.6563334866043106E-5 0.0 1.8094861153955846 0.002037145900389386 86-87 1.3969000459812932E-4 4.6563334866043106E-5 0.0 1.9979744949333271 0.0021535542375544937 88-89 1.3969000459812932E-4 4.6563334866043106E-5 0.0 2.2118049694719133 0.0022117584061370475 90-91 1.3969000459812932E-4 4.6563334866043106E-5 0.0 2.4467402755385343 0.0022117584061370475 92-93 1.3969000459812932E-4 4.6563334866043106E-5 0.0 2.7020586814427645 0.002235040073570069 94-95 1.3969000459812932E-4 4.6563334866043106E-5 0.0 2.960613239120186 0.002235040073570069 96-97 1.3969000459812932E-4 4.6563334866043106E-5 0.0 3.233940014783859 0.002444575080467263 98-99 1.3969000459812932E-4 4.6563334866043106E-5 0.0 3.537416549773295 0.002537701750199349 100-101 1.3969000459812932E-4 4.6563334866043106E-5 0.0 3.872893736649419 0.002537701750199349 102-103 1.3969000459812932E-4 4.6563334866043106E-5 0.0 4.23523796774325 0.0025842650850653923 104-105 1.3969000459812932E-4 4.6563334866043106E-5 0.0 4.607733005837877 0.0026308284199314355 106-107 1.3969000459812932E-4 4.6563334866043106E-5 0.0 4.989214767561653 0.0026308284199314355 108-109 1.3969000459812932E-4 4.6563334866043106E-5 0.0 5.393570767538371 0.002642469253647946 110-111 1.3969000459812932E-4 4.6563334866043106E-5 0.0 5.840555500584951 0.0027006734222305004 112-113 1.3969000459812932E-4 4.6563334866043106E-5 0.0 6.325128485702146 0.0027006734222305004 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9525 0.0 43.03881 1 ATCAACG 10575 0.0 38.14612 3 TCAACGC 10570 0.0 38.10787 4 CAACGCA 10890 0.0 36.87881 5 AACGCAG 11350 0.0 35.48487 6 TATCAAC 11475 0.0 35.154263 2 GGTATCA 6030 0.0 34.534782 1 GTACATG 13760 0.0 30.743778 1 ACGCAGA 13040 0.0 30.612259 7 CGCAGAG 12865 0.0 30.01483 8 TACATGG 13750 0.0 29.424374 2 ACATGGG 14210 0.0 27.299488 3 GCAGAGT 15535 0.0 23.97558 9 CATGGGG 8170 0.0 20.973526 4 AGAGTAC 13920 0.0 20.22023 10-11 CATGGGA 7095 0.0 19.37138 4 GAGTACA 12465 0.0 18.997517 1 AGTACAT 11095 0.0 18.769026 2 CAGAGTA 14865 0.0 18.754644 10-11 GAGTACT 7115 0.0 18.40136 12-13 >>END_MODULE