FastQCFastQC Report
Thu 26 May 2016
SRR1364575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295225
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56140.130703280968983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.1081433452263851No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121950.043.0222551
GGTATCA75750.042.2652971
TCAACGC143250.036.424334
ATCAACG143800.036.326443
CAACGCA145350.035.8579565
TATCAAC150000.034.5072752
AACGCAG150700.034.5060046
ACGCAGA171150.030.2439737
CGCAGAG173050.029.8431478
GTACATG132750.028.7433721
TACATGG130350.028.2853622
ACATGGG137000.026.6515243
GCAGAGT203050.024.5545799
GAGTACT115350.022.95270212-13
CATGGGG78650.022.7198094
AGAGTAC182700.021.98095310-11
GTACTTT124350.021.21970214-15
AGTACTT128700.020.7798612-13
CAGAGTA194050.020.77193510-11
TATACCG2801.5689184E-619.12415
Produced by FastQC (version 0.11.5)