##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364575_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4295225 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.250192946818853 31.0 30.0 34.0 26.0 34.0 2 30.127881310059426 31.0 30.0 34.0 26.0 34.0 3 30.404972964163694 31.0 30.0 34.0 26.0 34.0 4 33.9931605445582 35.0 35.0 37.0 30.0 37.0 5 33.45929840695191 35.0 35.0 37.0 28.0 37.0 6 33.50268845054683 36.0 35.0 37.0 28.0 37.0 7 33.29967603559767 36.0 33.0 37.0 27.0 37.0 8 33.23255661810499 36.0 33.0 37.0 26.0 37.0 9 34.50109738139446 38.0 34.0 39.0 27.0 39.0 10-11 34.445663498419755 38.0 34.0 39.0 27.0 39.0 12-13 34.33488769505672 38.0 34.0 39.0 26.0 39.0 14-15 35.33613291503937 39.0 34.0 41.0 26.0 41.0 16-17 35.33442636881654 39.0 34.0 41.0 26.0 41.0 18-19 35.33227432788736 39.0 34.0 41.0 26.0 41.0 20-21 35.32969960828595 39.0 34.0 41.0 26.0 41.0 22-23 35.286130645076796 39.0 34.0 41.0 26.0 41.0 24-25 35.24882177301538 39.0 34.0 41.0 25.5 41.0 26-27 35.12616964186975 39.0 34.0 41.0 25.0 41.0 28-29 34.979683718547925 39.0 34.0 41.0 24.5 41.0 30-31 34.8431879587216 39.0 34.0 41.0 24.0 41.0 32-33 34.669980967236874 38.0 34.0 41.0 24.0 41.0 34-35 34.50533627458398 38.0 33.0 40.0 24.0 41.0 36-37 34.32626905458969 38.0 33.0 40.0 23.0 41.0 38-39 34.17090839245907 38.0 33.0 40.0 22.0 41.0 40-41 34.21705871054485 38.0 33.0 40.0 22.0 41.0 42-43 34.1099353351687 38.0 33.0 41.0 20.5 41.0 44-45 33.97851916954292 38.0 33.0 40.5 19.0 41.0 46-47 33.79920225366541 38.0 33.0 40.0 18.0 41.0 48-49 33.57029561897223 38.0 33.0 40.0 15.0 41.0 50-51 33.4180445727523 38.0 32.5 40.0 14.5 41.0 52-53 33.23612034293896 38.0 32.0 40.0 13.0 41.0 54-55 33.05406317480458 38.0 32.0 40.0 10.5 41.0 56-57 32.841436595288954 37.5 32.0 40.0 9.0 41.0 58-59 32.640810434843345 37.0 31.0 40.0 8.0 41.0 60-61 32.41172464771927 37.0 31.0 40.0 7.5 41.0 62-63 32.236620316747086 36.5 31.0 40.0 7.0 41.0 64-65 32.10822867253752 36.0 31.0 40.0 4.0 41.0 66-67 31.811811139113782 36.0 31.0 39.0 2.0 41.0 68-69 31.52330192248369 35.0 31.0 39.0 2.0 41.0 70-71 31.206360435134364 35.0 30.0 39.0 2.0 40.5 72-73 30.91215489293343 35.0 30.0 38.0 2.0 40.0 74-75 30.553852941347657 35.0 30.0 37.0 2.0 39.5 76-77 30.23127985146296 35.0 30.0 37.0 2.0 39.0 78-79 29.930729822069857 35.0 30.0 36.0 2.0 39.0 80-81 29.640928589305567 34.5 29.0 36.0 2.0 37.5 82-83 29.33624850851818 34.0 29.0 35.0 2.0 37.0 84-85 29.100003492250114 34.0 29.0 35.0 2.0 37.0 86-87 28.881831568776956 34.0 29.0 35.0 2.0 36.0 88-89 28.692875111315473 34.0 29.0 35.0 2.0 36.0 90-91 28.542032023933555 34.0 29.0 35.0 2.0 36.0 92-93 28.42894400176941 34.0 29.0 35.0 2.0 35.0 94-95 28.319557997543782 34.0 29.0 35.0 2.0 35.0 96-97 28.20127467129196 34.0 29.0 35.0 2.0 35.0 98-99 28.113169391591825 34.0 29.0 35.0 2.0 35.0 100-101 28.00988423190869 34.0 28.5 35.0 2.0 35.0 102-103 26.70662025854292 32.0 25.0 34.0 2.0 35.0 104-105 27.669633371942098 33.5 27.0 35.0 2.0 35.0 106-107 27.840309878993533 34.0 28.0 35.0 2.0 35.0 108-109 27.84907018840689 34.0 28.0 35.0 2.0 35.0 110-111 27.763517161499106 34.0 28.0 35.0 2.0 35.0 112-113 27.677447281574306 34.0 27.5 35.0 2.0 35.0 114-115 27.574658836265854 34.0 27.0 35.0 2.0 35.0 116-117 27.45170404344359 34.0 27.0 35.0 2.0 35.0 118-119 27.31562875053111 34.0 27.0 35.0 2.0 35.0 120-121 27.112094011373095 34.0 27.0 35.0 2.0 35.0 122-123 26.81593071375772 34.0 26.5 35.0 2.0 35.0 124-125 24.973074402388697 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5971923927721186 1101 2 0.7458024653480315 1101 3 0.5575529358980269 1101 4 0.45828365647979297 1101 5 0.5165584837986188 1101 6 0.6893107437062866 1101 7 0.6800748059056261 1101 8 0.7679624330739827 1101 9 0.9540338887134965 1101 10-11 0.876389166704314 1101 12-13 0.8764728753735653 1101 14-15 0.9801512661256737 1101 16-17 1.0562356091950278 1101 18-19 1.0691898915281257 1101 20-21 1.1318871701414182 1101 22-23 1.0202560852966585 1101 24-25 0.9710903175766248 1101 26-27 0.961625140118386 1101 28-29 1.1270425597076326 1101 30-31 1.1156997084484317 1101 32-33 1.0900274684064115 1101 34-35 1.1004711131381697 1101 36-37 1.1445580803694853 1101 38-39 1.0740945311562342 1101 40-41 0.9300158238159213 1101 42-43 0.9482819758623933 1101 44-45 0.9233699550988419 1101 46-47 0.9831552106915993 1101 48-49 1.0210947843936609 1101 50-51 1.0253276511434635 1101 52-53 0.9766341875008351 1101 54-55 1.0298444420350847 1101 56-57 0.969892480527264 1101 58-59 1.0289223331088024 1101 60-61 0.9237736567147721 1101 62-63 0.9382674249800971 1101 64-65 0.8559117463540389 1101 66-67 1.025152031966897 1101 68-69 1.068626734305262 1101 70-71 1.120514579583336 1101 72-73 1.01217146177747 1101 74-75 1.091197154379131 1101 76-77 1.0764217354021959 1101 78-79 1.038081360402046 1101 80-81 0.8569906267994156 1101 82-83 1.0235571923105269 1101 84-85 1.013668711140813 1101 86-87 1.0699330899198323 1101 88-89 1.082021805001009 1101 90-91 0.9932003019291642 1101 92-93 0.9600397922901074 1101 94-95 0.9994339840447815 1101 96-97 1.0175685190877957 1101 98-99 1.03459163234821 1101 100-101 1.0030064540325263 1101 102-103 1.0076784992220915 1101 104-105 1.046413644858326 1101 106-107 1.0029869042802737 1101 108-109 1.0080541320056788 1101 110-111 0.897982089975141 1101 112-113 0.9322056346521741 1101 114-115 1.0221598134712053 1101 116-117 1.0657729977263735 1101 118-119 1.124264033603115 1101 120-121 1.1446584319991686 1101 122-123 1.0886700593354135 1101 124-125 1.3043749185925755 1102 1 0.16746189841163073 1102 2 -0.4485738589697448 1102 3 0.007168195765245855 1102 4 0.03721224830908909 1102 5 0.22591575804342767 1102 6 0.18112974190363218 1102 7 0.18593130900308807 1102 8 0.2552244910310222 1102 9 0.5349841344485284 1102 10-11 0.5880899026958701 1102 12-13 0.6290387207660615 1102 14-15 0.762408392711265 1102 16-17 0.7111379047894957 1102 18-19 0.8073176178484118 1102 20-21 0.6224163260323294 1102 22-23 0.729757218788869 1102 24-25 0.7391127579521353 1102 26-27 0.8602739835689732 1102 28-29 0.46862558019776657 1102 30-31 0.7409990658624181 1102 32-33 0.839440128951793 1102 34-35 0.871508848732816 1102 36-37 0.5012028429048883 1102 38-39 -0.08303623439903163 1102 40-41 -0.48933406624257003 1102 42-43 -0.1567769395006664 1102 44-45 -0.34566126515266404 1102 46-47 -0.15844151418150432 1102 48-49 -0.4549996233600311 1102 50-51 -0.46159433463388666 1102 52-53 -0.5919835232199944 1102 54-55 -0.5309182424255496 1102 56-57 -0.6381108300986256 1102 58-59 -0.5069965604792444 1102 60-61 -0.5759304364828779 1102 62-63 -0.5007682841310128 1102 64-65 -0.5539433475700157 1102 66-67 -0.6171506402406912 1102 68-69 -0.4083490074659153 1102 70-71 -0.9141174626369555 1102 72-73 -0.7103922286682227 1102 74-75 -0.5621290268516503 1102 76-77 -0.9539261426371333 1102 78-79 -0.7661923845253114 1102 80-81 -0.8153585128276539 1102 82-83 -1.0328661672147668 1102 84-85 -0.930928342000076 1102 86-87 -0.7082045334655795 1102 88-89 -0.7713154451544817 1102 90-91 -0.7692883380199191 1102 92-93 -0.6056152833450952 1102 94-95 -0.37208559912623684 1102 96-97 -0.6483370231460981 1102 98-99 -0.8521219070673389 1102 100-101 -0.6572409064027944 1102 102-103 -0.5848615235825889 1102 104-105 -0.5322312613401969 1102 106-107 -0.46128203062929884 1102 108-109 -0.4379535532357828 1102 110-111 -0.3789688907462967 1102 112-113 -0.21661149813104075 1102 114-115 -0.2169928977289004 1102 116-117 -0.3218812286783681 1102 118-119 -0.03979010532892957 1102 120-121 0.02086072193046462 1102 122-123 3.138326546228143E-4 1102 124-125 0.19673689474008782 1103 1 0.18129093461491053 1103 2 -0.2380979265438974 1103 3 0.14966180724370304 1103 4 0.08940124911448777 1103 5 0.1814906752660832 1103 6 0.22984731456374874 1103 7 0.38643070743802355 1103 8 0.3835074950779145 1103 9 0.5919563232504714 1103 10-11 0.5687083215243547 1103 12-13 0.4869858426998164 1103 14-15 0.5303480820849416 1103 16-17 0.4171780406139334 1103 18-19 0.5391522172856185 1103 20-21 0.5429139098288545 1103 22-23 0.5268136515577595 1103 24-25 0.4721215278758777 1103 26-27 0.5587490658491561 1103 28-29 0.5879427789926481 1103 30-31 0.5969095669014877 1103 32-33 0.6605756948703956 1103 34-35 0.6578085772827933 1103 36-37 0.6936195186861767 1103 38-39 0.8131794987645975 1103 40-41 0.6308884631148004 1103 42-43 0.553307354182401 1103 44-45 0.5439647204694396 1103 46-47 0.6185579439127764 1103 48-49 0.5872026516496689 1103 50-51 0.527482163561416 1103 52-53 0.5456031071207619 1103 54-55 0.5513027755617443 1103 56-57 0.6105258295746339 1103 58-59 0.6116018674970221 1103 60-61 0.6546073179393659 1103 62-63 0.584962443414696 1103 64-65 0.5855093773705704 1103 66-67 0.5718652042870502 1103 68-69 0.5583488974967494 1103 70-71 0.5747595496230424 1103 72-73 0.49152233914954024 1103 74-75 0.6141356269749991 1103 76-77 0.5943149010036812 1103 78-79 0.4759957213899142 1103 80-81 0.45277009780563304 1103 82-83 0.5438848345650698 1103 84-85 0.6160225683020997 1103 86-87 0.6416174828214203 1103 88-89 0.6542423697225708 1103 90-91 0.6050887189845824 1103 92-93 0.5671590502103072 1103 94-95 0.6090196648166497 1103 96-97 0.5936317103854378 1103 98-99 0.6233276509563481 1103 100-101 0.5613545386062029 1103 102-103 0.30268620389352563 1103 104-105 0.4183142487969178 1103 106-107 0.5115880064589291 1103 108-109 0.5623284614603072 1103 110-111 0.4785224586486585 1103 112-113 0.44129945532472803 1103 114-115 0.4842728747104701 1103 116-117 0.5342247810063299 1103 118-119 0.4581152302051734 1103 120-121 0.5089604226394187 1103 122-123 0.5662943339919835 1103 124-125 0.9130475111205563 1104 1 0.2675005179304648 1104 2 0.45118447498029113 1104 3 0.19288416880803894 1104 4 -0.018338792923131564 1104 5 0.21688932768770286 1104 6 0.3523533484275916 1104 7 0.381765988056749 1104 8 0.3473277796202723 1104 9 0.3428365902431523 1104 10-11 0.3744913231884226 1104 12-13 0.3232937487774663 1104 14-15 0.4371292221095402 1104 16-17 0.43123098371103197 1104 18-19 0.4370019865981618 1104 20-21 0.3695678424610662 1104 22-23 0.4766368792899769 1104 24-25 0.542796903218381 1104 26-27 0.3934609662426283 1104 28-29 0.6631959601050283 1104 30-31 0.4832584253371124 1104 32-33 0.5462784057959098 1104 34-35 0.6549151981149777 1104 36-37 0.788347076515727 1104 38-39 0.8927692006611139 1104 40-41 0.7934871337168516 1104 42-43 0.7697632221033999 1104 44-45 0.66312722387827 1104 46-47 0.6934035725384291 1104 48-49 0.6529594771972569 1104 50-51 0.5375135201743646 1104 52-53 0.6685455641880367 1104 54-55 0.29695811532253913 1104 56-57 0.49854833157355216 1104 58-59 0.5483463127247461 1104 60-61 0.6282581394909812 1104 62-63 0.5626915493639686 1104 64-65 0.6115916234334904 1104 66-67 0.6554754723371659 1104 68-69 0.37648940072377357 1104 70-71 -0.013440420323583169 1104 72-73 0.01908137454758574 1104 74-75 0.4724344623140553 1104 76-77 0.6186083220978063 1104 78-79 0.6523883334546738 1104 80-81 0.570391693379996 1104 82-83 0.6297503345919715 1104 84-85 0.5995612045097332 1104 86-87 0.6143241971529854 1104 88-89 0.6022284691101838 1104 90-91 0.7167968729097964 1104 92-93 0.727318736579246 1104 94-95 0.6364884241503574 1104 96-97 0.7598263736856339 1104 98-99 0.8084808906913565 1104 100-101 0.7613856354986481 1104 102-103 0.5839272644065439 1104 104-105 0.6660560022071422 1104 106-107 0.588403058560715 1104 108-109 0.5855236590426749 1104 110-111 0.594305789601222 1104 112-113 0.6964397183102911 1104 114-115 0.6420853602171377 1104 116-117 0.6176211970488943 1104 118-119 0.6676807131647209 1104 120-121 0.6968558239168701 1104 122-123 0.6872150524531051 1104 124-125 1.0022391959831154 1105 1 0.2623258286506456 1105 2 0.0031582512882160074 1105 3 0.24243644589177293 1105 4 0.3392721016739557 1105 5 0.017182145198773924 1105 6 0.5408790777524359 1105 7 0.623594048200907 1105 8 0.6301584205413846 1105 9 0.7456697314756582 1105 10-11 0.7359686067296138 1105 12-13 0.6825999546201444 1105 14-15 0.8013739562994289 1105 16-17 0.7345505145049103 1105 18-19 0.7723516820598633 1105 20-21 0.7565561099197993 1105 22-23 0.7590132020575808 1105 24-25 0.7485954486654478 1105 26-27 0.8200760231876387 1105 28-29 0.7170232231256648 1105 30-31 0.7852318801501852 1105 32-33 0.8222125687941713 1105 34-35 0.8770752136349103 1105 36-37 0.8984031920642863 1105 38-39 0.9312591154594685 1105 40-41 0.7369731152553314 1105 42-43 0.7091427498991081 1105 44-45 0.817766798755521 1105 46-47 0.6716813365582652 1105 48-49 0.6881091064126537 1105 50-51 0.5704558346286674 1105 52-53 0.6901492833874556 1105 54-55 0.5858577293817504 1105 56-57 0.51503704471601 1105 58-59 0.29491081873779734 1105 60-61 0.4421297743125052 1105 62-63 0.6595659626358668 1105 64-65 0.4698090180686023 1105 66-67 0.6088954840505991 1105 68-69 0.6437584048356015 1105 70-71 0.7063750480613464 1105 72-73 0.6957499144575898 1105 74-75 0.6334918092797466 1105 76-77 0.6025914256143743 1105 78-79 0.5171614214696731 1105 80-81 0.5577178611135949 1105 82-83 0.5815382087982002 1105 84-85 0.48726583139015744 1105 86-87 0.6767944129513523 1105 88-89 0.6180452120844215 1105 90-91 0.3944291166021614 1105 92-93 0.5614148776857206 1105 94-95 0.5346249619116712 1105 96-97 0.5950076058449625 1105 98-99 0.4609992884638956 1105 100-101 0.5032678215975039 1105 102-103 0.0987653770517305 1105 104-105 0.3191458800427718 1105 106-107 0.395000573828046 1105 108-109 0.46879971730269077 1105 110-111 0.3857001118551686 1105 112-113 0.41560507817693093 1105 114-115 0.3922709775662483 1105 116-117 0.09192489431317696 1105 118-119 0.22094974630294217 1105 120-121 0.30260163502266124 1105 122-123 0.31437035393995316 1105 124-125 0.6106372247971379 1106 1 -0.20307510594699707 1106 2 -0.67748068508633 1106 3 -0.08938167463506375 1106 4 -0.18218858544558714 1106 5 0.05686115630517463 1106 6 0.1509452546967367 1106 7 0.2677128101212176 1106 8 0.3103763534894739 1106 9 0.3445727628501487 1106 10-11 0.28701583402501996 1106 12-13 0.24265605594862905 1106 14-15 0.23962410275169077 1106 16-17 0.19533923296148714 1106 18-19 0.3084659290555365 1106 20-21 0.2545260980687729 1106 22-23 0.20350124091574884 1106 24-25 0.27380892811643065 1106 26-27 0.38183964174953644 1106 28-29 0.37760325647631987 1106 30-31 0.39415724643065886 1106 32-33 0.300278440098964 1106 34-35 0.37253950100795663 1106 36-37 0.34225579292156993 1106 38-39 0.2225555625501272 1106 40-41 0.26594847007432065 1106 42-43 0.14882829704624356 1106 44-45 0.21830867211588867 1106 46-47 0.15063528537562831 1106 48-49 0.352664419670667 1106 50-51 0.32580686683617444 1106 52-53 0.09864780313341726 1106 54-55 0.2425011559600989 1106 56-57 -0.19745057613519634 1106 58-59 0.29791458829161144 1106 60-61 0.27359253273894524 1106 62-63 0.11010967133145755 1106 64-65 0.1954254659683059 1106 66-67 0.017857238661040498 1106 68-69 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-0.9770405739912889 2216 58-59 -1.1520275014763577 2216 60-61 -1.0403642376112892 2216 62-63 -1.2953821646157486 2216 64-65 -1.0313954666371785 2216 66-67 -0.9515860887502932 2216 68-69 -0.7899187849150167 2216 70-71 -0.8815510979081012 2216 72-73 -0.7902102199062107 2216 74-75 -0.7545773956644872 2216 76-77 -0.8645894888756978 2216 78-79 -0.9200007896163456 2216 80-81 -0.8350715999058522 2216 82-83 -0.7557684477409552 2216 84-85 -0.7743412681676318 2216 86-87 -0.9367532130201681 2216 88-89 -0.8494318550184481 2216 90-91 -0.8701167402146055 2216 92-93 -0.8972427818079467 2216 94-95 -0.7879718935638422 2216 96-97 -0.860538846591151 2216 98-99 -0.9358203987063192 2216 100-101 -0.9648598205771428 2216 102-103 -0.8043695721364621 2216 104-105 -0.7951751113819512 2216 106-107 -0.8558843391564892 2216 108-109 -0.8128235253936964 2216 110-111 -0.7863378439228406 2216 112-113 -0.7176648036707824 2216 114-115 -0.9389106329737054 2216 116-117 -0.9771025765003039 2216 118-119 -0.9782545535670266 2216 120-121 -1.0756087230654714 2216 122-123 -1.0631621819452945 2216 124-125 -1.3698952873438834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114706.0 3 39767.0 4 19413.0 5 15284.0 6 16075.0 7 19086.0 8 21783.0 9 24073.0 10 26578.0 11 28784.0 12 31264.0 13 34672.0 14 37472.0 15 33827.0 16 26509.0 17 23576.0 18 22060.0 19 21082.0 20 21032.0 21 21690.0 22 24774.0 23 30621.0 24 39855.0 25 51950.0 26 64485.0 27 74317.0 28 86974.0 29 103276.0 30 120600.0 31 143146.0 32 173745.0 33 215452.0 34 277785.0 35 378207.0 36 604872.0 37 936250.0 38 368978.0 39 1205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46180546330723 19.805898641654046 14.10187571341944 24.630420181619286 2 15.125307754541382 20.93415362408256 39.5073133537824 24.433225267593663 3 19.206560773882625 24.9159939234848 29.544901605853013 26.332543696779563 4 12.966581170966387 17.327071623894735 37.51076075947774 32.195586445661135 5 13.141663125913079 38.47791442823135 34.821435431205586 13.55898701464999 6 31.5642137489887 37.75134015098162 17.37461949024789 13.30982660978179 7 27.51820779681584 32.448037758041764 21.6347879858575 18.398966459284892 8 25.273894509257637 36.42746332764687 19.889485268078435 18.40915689501706 9 25.74683056256874 16.263965966672597 19.972089020843363 38.0171144499153 10-11 24.57058756628474 26.80331220859189 28.044732719694927 20.581367505428442 12-13 25.515275684044493 23.908468124487076 28.26209337112724 22.314162820341192 14-15 22.393796550260603 25.5303508106564 26.054630923702916 26.02122171538009 16-17 22.207757799205766 28.619837945172915 26.740257118797135 22.43214713682418 18-19 22.08874490748701 27.41769298815514 28.71864765717919 21.774914447178663 20-21 22.774478985019 26.701013742400193 28.75429493659628 21.770212335984525 22-23 22.360982253549 26.680313604060323 28.4804172074804 22.478286934910276 24-25 22.213225151185327 26.79256616358864 28.63698642096747 22.357222264258567 26-27 22.13918944874832 27.026570202957934 28.471965962202212 22.36227438609153 28-29 22.097596749879227 27.212066888230535 28.457717581733206 22.232618780157036 30-31 22.373449586459383 26.88136244317818 28.398302766444132 22.346885203918305 32-33 22.18839525286801 26.91134923083191 28.3485032798049 22.55175223649518 34-35 22.49851870390957 26.994348375230633 28.31386015866456 22.193272762195228 36-37 22.535222252617732 26.763638691803106 28.259159881030683 22.441979174548482 38-39 22.394484572985117 27.019201555215382 28.199919678247355 22.386394193552142 40-41 22.666123427759896 26.8638197067674 28.173460063209728 22.29659680226298 42-43 22.466937122036683 26.881746590690824 28.002956771763994 22.648359515508503 44-45 22.508867405083553 26.978516841376177 28.060124906145777 22.45249084739449 46-47 22.628756351529898 27.144922559353702 27.537300141436127 22.689020947680273 48-49 22.570703513785656 27.18515328067796 27.55855630380248 22.685586901733902 50-51 22.787736840175334 27.32632593538519 27.414016386811163 22.471920837628318 52-53 22.90882316991543 27.164525723332307 27.35116321030912 22.575487896443143 54-55 22.621585597960525 27.22598932535548 27.506079425408448 22.646345651275546 56-57 22.66837720965347 27.262128100965498 27.389010188169433 22.680484501211602 58-59 22.627114224463075 27.222333716397312 27.42391958264031 22.726632476499304 60-61 22.700487168891037 27.24448661013102 27.48188977294554 22.573136448032407 62-63 22.660233165899342 27.161755204907777 27.594794219161976 22.583217410030905 64-65 22.411338287604362 27.10205643883143 27.637978202030094 22.84862707153411 66-67 22.484759238456657 27.319104354253852 27.402569132001236 22.793567275288257 68-69 22.58757634060529 27.192085907510293 27.59483556620097 22.625502185683448 70-71 22.43544866683352 27.041610160119667 27.446699532620528 23.076241640426286 72-73 22.521800371342596 27.244195589288108 27.269898550134158 22.96410548923514 74-75 22.470133880677075 27.292552933901536 27.292250268208367 22.945062917213015 76-77 22.61173745263636 27.07557811290445 27.33363211473206 22.97905231972714 78-79 22.646380573776696 27.153094424622694 27.283087614734967 22.917437386865647 80-81 22.64748162580207 27.22528977842004 27.15603840221046 22.971190193567434 82-83 22.76876065863837 27.202835707093342 27.246209453521057 22.782194180747226 84-85 22.63965217188855 27.194954862667263 27.144491848506192 23.020901116937996 86-87 22.61950188872527 27.201275835375327 27.184152168978343 22.99507010692106 88-89 22.682318489393683 27.174555827281583 27.0694495406009 23.073676142723837 90-91 22.47297871473555 27.32441257442858 27.208725968953896 22.993882741881976 92-93 22.569621041517394 27.187120754855354 27.28602352316666 22.95723468046059 94-95 22.40008381400276 27.15665651973994 27.307207422195578 23.13605224406172 96-97 22.490660726598904 26.939401739341807 27.32050486384958 23.249432670209703 98-99 22.26751174162545 27.055789964453535 27.18074681918217 23.495951474738845 100-101 22.29060565729643 27.008317649922525 27.387629773021555 23.313446919759492 102-103 22.287144445285172 26.76660710440082 27.405956614612737 23.540291835701275 104-105 22.160678427788998 26.891408482675526 27.259363595620716 23.688549493914753 106-107 22.195026210922258 26.874856233394084 27.25683304634337 23.673284509340288 108-109 22.12544162412912 26.794044549470637 27.256523232193892 23.823990594206357 110-111 22.0696053631774 26.935135959433925 27.209802070443505 23.78545660694517 112-113 22.21803281551025 26.848081299582677 27.303587122909747 23.630298761997334 114-115 22.1034288075712 26.979797333085 27.125889796227202 23.7908840631166 116-117 21.86685214395055 26.884319214942177 27.309046673922786 23.93978196718449 118-119 21.769709386586268 26.972894318691104 27.21552421584434 24.041872078878292 120-121 21.740354757504765 27.019414169928552 27.232699387395776 24.007531685170903 122-123 21.807374684186488 26.897456693546783 27.19618370739304 24.098984914873686 124-125 21.617585225971986 26.862799032596342 27.181822280645157 24.337793460786518 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 103.0 2 122.5 3 216.0 4 190.5 5 148.0 6 269.0 7 606.5 8 1039.5 9 1248.5 10 1059.5 11 835.0 12 868.5 13 1008.0 14 1176.0 15 1411.0 16 1716.0 17 2060.0 18 2524.0 19 3131.0 20 3800.0 21 4818.5 22 6279.0 23 8225.0 24 11103.5 25 14871.0 26 20808.5 27 28357.5 28 35611.5 29 44122.0 30 53319.5 31 62365.5 32 73221.0 33 82173.0 34 88934.0 35 96207.0 36 104700.0 37 113806.0 38 121474.0 39 127858.5 40 134919.0 41 143085.5 42 147188.0 43 150718.0 44 154914.5 45 158428.0 46 161479.0 47 163933.0 48 168667.5 49 167759.5 50 160811.5 51 155121.0 52 150442.5 53 146382.0 54 141486.5 55 131533.5 56 119491.0 57 108735.0 58 100420.5 59 89587.5 60 73921.5 61 59543.5 62 47203.5 63 35619.0 64 25622.5 65 18926.5 66 14282.5 67 10731.0 68 8173.0 69 6305.5 70 4693.5 71 3503.0 72 2584.5 73 2125.5 74 1657.0 75 1164.5 76 822.5 77 481.5 78 306.0 79 202.0 80 140.0 81 91.0 82 55.5 83 33.5 84 23.0 85 19.0 86 11.5 87 4.5 88 5.0 89 4.5 90 2.5 91 3.0 92 2.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09943600160643505 2 0.0 3 0.0 4 0.0971078348631329 5 0.0 6 0.0 7 0.002025505066672875 8 0.005401346844461001 9 0.0032827151080560392 10-11 5.704008521090281E-4 12-13 0.0 14-15 4.6563334866043106E-5 16-17 2.7938000919625863E-4 18-19 0.0016646392214610412 20-21 0.0011873650390840991 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 5.820416858255389E-5 52-53 0.0 54-55 0.0 56-57 0.006809887724158805 58-59 7.682950252897113E-4 60-61 0.0 62-63 0.0 64-65 1.978941731806832E-4 66-67 0.0 68-69 2.3281667433021553E-5 70-71 0.0 72-73 0.0 74-75 0.0013270550436822286 76-77 0.0 78-79 0.0 80-81 8.148583601557544E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0011408017042180561 90-91 0.0 92-93 0.0022699625747196013 94-95 0.0 96-97 0.007554901082015494 98-99 0.0033176376092055714 100-101 0.0018974558957912566 102-103 0.0 104-105 0.0 106-107 0.0016064350528784872 108-109 0.0 110-111 2.3281667433021556E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.730625287383082E-4 122-123 0.0038763976275980883 124-125 0.08221920853971562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4295225.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.906328403333255 #Duplication Level Percentage of deduplicated Percentage of total 1 68.8882016462329 32.312926095332436 2 13.970210841913437 13.105825952291966 3 6.1483561798984905 8.651904423449464 4 3.4003379866258157 6.37989481131998 5 2.055592772850398 4.821015483341959 6 1.3327274486158918 3.7508010826148084 7 0.8853571106528719 2.907019597055691 8 0.6348819062484751 2.3823983353460165 9 0.4905251175753145 2.0707859029564224 >10 2.0618532988921854 16.129027419863444 >50 0.0906745191016974 2.924111404132374 >100 0.0383928367490202 3.058556522632113 >500 0.0017927596010019462 0.5721743926695858 >1k 0.0010457761767888405 0.8024221647622436 >5k 4.979886556136522E-5 0.13113641223144987 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5614 0.130703280968983 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4645 0.1081433452263851 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6563334866043106E-5 2 0.0 0.0 0.0 2.3281667433021553E-5 4.6563334866043106E-5 3 0.0 0.0 0.0 2.3281667433021553E-5 4.6563334866043106E-5 4 0.0 0.0 0.0 4.6563334866043106E-5 4.6563334866043106E-5 5 0.0 0.0 0.0 4.6563334866043106E-5 4.6563334866043106E-5 6 0.0 0.0 0.0 4.6563334866043106E-5 4.6563334866043106E-5 7 0.0 0.0 0.0 4.6563334866043106E-5 4.6563334866043106E-5 8 0.0 0.0 0.0 4.6563334866043106E-5 4.6563334866043106E-5 9 0.0 0.0 0.0 6.984500229906466E-5 4.6563334866043106E-5 10-11 0.0 0.0 0.0 2.0953500689719398E-4 4.6563334866043106E-5 12-13 0.0 0.0 0.0 3.026616766292802E-4 2.4445750804672633E-4 14-15 0.0 0.0 0.0 3.026616766292802E-4 3.3758417777881254E-4 16-17 0.0 0.0 0.0 3.3758417777881254E-4 4.423516812274095E-4 18-19 0.0 0.0 0.0 4.5399251494392025E-4 4.6563334866043106E-4 20-21 0.0 0.0 0.0 4.7727418237694186E-4 5.471191846760065E-4 22-23 1.1640833716510776E-5 0.0 0.0 5.936825195420497E-4 6.053233532585604E-4 24-25 2.3281667433021553E-5 0.0 0.0 7.799358590062221E-4 6.053233532585604E-4 26-27 2.3281667433021553E-5 0.0 0.0 8.963441961713298E-4 6.868091892741359E-4 28-29 2.3281667433021553E-5 0.0 0.0 0.0014201817134143148 7.682950252897113E-4 30-31 2.3281667433021553E-5 0.0 0.0 0.0029218492628442045 7.799358590062221E-4 32-33 2.3281667433021553E-5 0.0 0.0 0.0073570069088348105 7.915766927227328E-4 34-35 2.3281667433021553E-5 0.0 0.0 0.012385847074367465 7.915766927227328E-4 36-37 2.3281667433021553E-5 0.0 0.0 0.02054607150964152 7.915766927227328E-4 38-39 2.3281667433021553E-5 0.0 0.0 0.03733215372885006 8.148583601557544E-4 40-41 2.3281667433021553E-5 0.0 0.0 0.0562834310193296 8.148583601557544E-4 42-43 2.3281667433021553E-5 0.0 0.0 0.07531619414582472 8.381400275887759E-4 44-45 2.3281667433021553E-5 0.0 0.0 0.0991449807635223 9.312666973208621E-4 46-47 2.3281667433021553E-5 0.0 0.0 0.12839839589311386 0.0010011116996199267 48-49 2.3281667433021553E-5 0.0 0.0 0.16071335029014777 0.0010360342007694591 50-51 2.3281667433021553E-5 0.0 0.0 0.19721900482512558 0.0010709567019189915 52-53 2.3281667433021553E-5 0.0 0.0 0.23253729432101927 0.0011640833716510778 54-55 2.3281667433021553E-5 0.0 0.0 0.27139439726673226 0.0011640833716510778 56-57 2.3281667433021553E-5 0.0 0.0 0.31609519873813363 0.0011873650390840991 58-59 2.3281667433021553E-5 0.0 0.0 0.3683276196241175 0.0012222875402336316 60-61 2.3281667433021553E-5 0.0 0.0 0.42571692984651566 0.0012339283739501424 62-63 4.6563334866043106E-5 0.0 0.0 0.48778585522295104 0.0012804917088161854 64-65 4.6563334866043106E-5 0.0 0.0 0.5507394839618414 0.0012804917088161854 66-67 4.6563334866043106E-5 0.0 0.0 0.627080071474719 0.0013154142099657178 68-69 4.6563334866043106E-5 0.0 0.0 0.7128264526305375 0.0013503367111152502 70-71 4.6563334866043106E-5 0.0 0.0 0.8109819625281562 0.0013619775448317608 72-73 4.6563334866043106E-5 0.0 0.0 0.9148414809468655 0.0014667450482803578 74-75 4.6563334866043106E-5 0.0 0.0 1.0202492302498705 0.001559871718012444 76-77 4.6563334866043106E-5 0.0 0.0 1.1377634466180466 0.001559871718012444 78-79 4.6563334866043106E-5 0.0 0.0 1.2748459044636777 0.0015947942191619764 80-81 4.6563334866043106E-5 0.0 0.0 1.4260486936074361 0.0016297167203115086 82-83 4.6563334866043106E-5 0.0 0.0 1.5969594142332473 0.0016762800551775518 84-85 4.6563334866043106E-5 0.0 0.0 1.7707221391196037 0.0016762800551775518 86-87 4.6563334866043106E-5 0.0 0.0 1.9548452060136547 0.0017577658911931272 88-89 4.6563334866043106E-5 0.0 0.0 2.1642521637399668 0.0018392517272087026 90-91 4.6563334866043106E-5 0.0 0.0 2.3955788113544694 0.0018392517272087026 92-93 4.6563334866043106E-5 0.0 0.0 2.646881129626504 0.0020022233992398534 94-95 4.6563334866043106E-5 0.0 0.0 2.901757183849507 0.0020604275678224072 96-97 5.820416858255388E-5 0.0 0.0 3.170776851038071 0.00224668090728658 98-99 6.984500229906466E-5 0.0 0.0 3.469166341693392 0.0023747300781681983 100-101 6.984500229906466E-5 0.0 0.0 3.799312026727354 0.0023747300781681983 102-103 6.984500229906466E-5 0.0 0.0 4.156406241815039 0.0024911384153333063 104-105 6.984500229906466E-5 0.0 2.3281667433021553E-5 4.5231157855525606 0.0025842650850653923 106-107 6.984500229906466E-5 2.3281667433021553E-5 2.3281667433021553E-5 4.900697867981306 0.0025842650850653923 108-109 6.984500229906466E-5 2.3281667433021553E-5 2.3281667433021553E-5 5.299454626940381 0.002607546752498414 110-111 6.984500229906466E-5 2.3281667433021553E-5 2.3281667433021553E-5 5.741480364823729 0.002642469253647946 112-113 6.984500229906466E-5 2.3281667433021553E-5 2.3281667433021553E-5 6.219150335547032 0.002654110087364457 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12195 0.0 43.022255 1 GGTATCA 7575 0.0 42.265297 1 TCAACGC 14325 0.0 36.42433 4 ATCAACG 14380 0.0 36.32644 3 CAACGCA 14535 0.0 35.857956 5 TATCAAC 15000 0.0 34.507275 2 AACGCAG 15070 0.0 34.506004 6 ACGCAGA 17115 0.0 30.243973 7 CGCAGAG 17305 0.0 29.843147 8 GTACATG 13275 0.0 28.743372 1 TACATGG 13035 0.0 28.285362 2 ACATGGG 13700 0.0 26.651524 3 GCAGAGT 20305 0.0 24.554579 9 GAGTACT 11535 0.0 22.952702 12-13 CATGGGG 7865 0.0 22.719809 4 AGAGTAC 18270 0.0 21.980953 10-11 GTACTTT 12435 0.0 21.219702 14-15 AGTACTT 12870 0.0 20.77986 12-13 CAGAGTA 19405 0.0 20.771935 10-11 TATACCG 280 1.5689184E-6 19.1241 5 >>END_MODULE