FastQCFastQC Report
Thu 26 May 2016
SRR1364574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879895
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48410.1680964062925905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33180.11521253379029443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.060.1234661
GTATCAA73000.054.6084981
ATCAACG88750.044.045883
TCAACGC89700.043.1814084
CAACGCA90650.042.925785
AACGCAG93700.041.395056
TATCAAC100400.039.763952
GTACATG125400.037.4833031
TACATGG126200.036.1606942
ACGCAGA111300.034.2618527
ACATGGG128600.034.144733
CGCAGAG110950.033.6775638
GCAGAGT122350.028.9844919
CATGGGA82550.027.2447624
CATGGGG59650.025.2358744
GAGTACT60100.024.16095512-13
CAGAGTA117300.024.07149510-11
AGAGTAC112200.023.44197810-11
GAGTACA92800.022.2479381
GTGGTAT25300.022.1062771
Produced by FastQC (version 0.11.5)