##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364574_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2879895 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.000080905727465 31.0 26.0 31.0 2.0 34.0 2 26.05132444064801 31.0 26.0 31.0 2.0 34.0 3 26.214072388055815 31.0 26.0 31.0 2.0 34.0 4 29.320678010830257 35.0 28.0 37.0 2.0 37.0 5 29.36367055048882 35.0 28.0 37.0 2.0 37.0 6 29.360490573441044 35.0 29.0 37.0 2.0 37.0 7 29.067954908078246 35.0 28.0 37.0 2.0 37.0 8 28.981334388927372 35.0 28.0 37.0 2.0 37.0 9 29.85453427989562 35.0 28.0 39.0 2.0 39.0 10-11 29.748858204899832 35.0 27.0 39.0 2.0 39.0 12-13 29.62901546757781 35.0 27.0 39.0 2.0 39.0 14-15 30.137354139647453 36.0 27.0 40.0 2.0 41.0 16-17 30.218382267409055 36.5 27.0 40.0 2.0 41.0 18-19 30.004207792297983 36.5 27.0 40.0 2.0 41.0 20-21 29.6890466492702 36.0 25.5 40.0 2.0 41.0 22-23 29.559945241059136 36.0 25.0 40.0 2.0 41.0 24-25 29.37944438946559 36.0 25.0 40.0 2.0 41.0 26-27 29.217150798900654 36.0 24.5 40.0 2.0 41.0 28-29 29.24599021839338 36.0 24.5 40.0 2.0 41.0 30-31 29.16167551247528 36.0 24.5 40.0 2.0 41.0 32-33 29.10899598770094 36.0 25.0 40.0 2.0 41.0 34-35 28.632398403414015 35.0 23.5 40.0 2.0 41.0 36-37 28.5040555298023 35.0 23.0 40.0 2.0 41.0 38-39 28.288836398549254 35.0 22.0 40.0 2.0 41.0 40-41 28.479094550322145 35.0 23.5 40.0 2.0 41.0 42-43 28.36695053118256 35.0 23.0 40.0 2.0 41.0 44-45 28.21507554962941 35.0 22.5 39.5 2.0 41.0 46-47 28.162280222021984 35.0 22.5 39.0 2.0 41.0 48-49 28.002357551230165 35.0 22.0 39.0 2.0 41.0 50-51 27.838898987636703 35.0 21.0 39.0 2.0 41.0 52-53 27.762231435521088 34.5 20.5 39.0 2.0 40.5 54-55 27.772293087074353 34.5 20.0 39.0 2.0 41.0 56-57 27.642834721404775 34.0 19.5 39.0 2.0 41.0 58-59 27.43900281086637 34.0 18.5 39.0 2.0 41.0 60-61 27.23628882303001 34.0 18.0 39.0 2.0 40.5 62-63 26.952954361183306 34.0 16.0 38.5 2.0 40.0 64-65 26.68437720819683 33.5 13.5 38.0 2.0 40.0 66-67 26.457632830363607 33.0 13.0 38.0 2.0 40.0 68-69 26.426080291121725 33.0 13.0 37.5 2.0 40.0 70-71 26.214611817444734 33.0 9.5 37.0 2.0 40.0 72-73 25.957358341189522 33.0 8.0 36.5 2.0 39.0 74-75 25.67333565980704 33.0 7.0 36.0 2.0 39.0 76-77 25.386533189578092 33.0 4.5 36.0 2.0 39.0 78-79 25.091030749384963 32.5 2.0 35.0 2.0 37.5 80-81 24.803586415476953 32.0 2.0 35.0 2.0 37.0 82-83 24.569393154958775 32.0 2.0 35.0 2.0 37.0 84-85 24.32793435177324 32.0 2.0 35.0 2.0 36.0 86-87 24.127924108344228 32.0 2.0 35.0 2.0 36.0 88-89 23.930829075365594 32.0 2.0 35.0 2.0 35.5 90-91 23.784139873155098 32.0 2.0 35.0 2.0 35.0 92-93 23.65497717798739 32.0 2.0 35.0 2.0 35.0 94-95 23.522577732868733 32.0 2.0 35.0 2.0 35.0 96-97 23.397017252365103 31.5 2.0 35.0 2.0 35.0 98-99 23.29527639028506 31.0 2.0 35.0 2.0 35.0 100-101 23.192001097262228 31.0 2.0 35.0 2.0 35.0 102-103 22.194408823932818 29.5 2.0 34.0 2.0 35.0 104-105 22.933112839183373 31.0 2.0 34.5 2.0 35.0 106-107 23.058317751168012 31.0 2.0 35.0 2.0 35.0 108-109 23.001255601332687 31.5 2.0 35.0 2.0 35.0 110-111 22.905147062653327 31.0 2.0 35.0 2.0 35.0 112-113 22.797900965139355 31.0 2.0 35.0 2.0 35.0 114-115 22.66080673080095 31.0 2.0 35.0 2.0 35.0 116-117 22.53690724835454 31.0 2.0 35.0 2.0 35.0 118-119 22.370374093499937 31.0 2.0 35.0 2.0 35.0 120-121 22.168900949513784 31.0 2.0 35.0 2.0 35.0 122-123 21.899413346667153 31.0 2.0 35.0 2.0 35.0 124-125 20.579639709086617 28.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47557058198432856 1101 2 0.433587752107087 1101 3 0.4560801233807048 1101 4 0.49254932526921635 1101 5 0.6006233531077925 1101 6 0.7288690723611957 1101 7 0.6997856753031719 1101 8 0.5850830145999915 1101 9 0.882047855664382 1101 10-11 0.9234703851616182 1101 12-13 0.6787278133447323 1101 14-15 0.9257407617249704 1101 16-17 0.7709554857578951 1101 18-19 1.1088154452091104 1101 20-21 1.4577239726853648 1101 22-23 1.378687818487684 1101 24-25 1.502979281321302 1101 26-27 1.5975105439620272 1101 28-29 1.4285341016947264 1101 30-31 1.4567582929079634 1101 32-33 1.4864526164323486 1101 34-35 1.807492270347776 1101 36-37 1.7000027522861672 1101 38-39 1.4843088739287573 1101 40-41 1.2546411008453084 1101 42-43 1.3790249603862534 1101 44-45 1.3117774415586645 1101 46-47 1.18420570286224 1101 48-49 1.202719439404074 1101 50-51 1.0941655569414728 1101 52-53 0.9287943638969303 1101 54-55 0.8087133829693158 1101 56-57 0.7698211851725993 1101 58-59 0.9111575330730055 1101 60-61 0.7972401163436906 1101 62-63 0.7612728238602742 1101 64-65 0.9520270207362209 1101 66-67 0.9128097830939979 1101 68-69 0.7537695612655604 1101 70-71 0.7097742256055035 1101 72-73 0.5390875118192575 1101 74-75 0.5594222796529209 1101 76-77 0.6847939330132213 1101 78-79 0.6530035191084771 1101 80-81 0.7972673964852461 1101 82-83 0.6075416591802032 1101 84-85 0.6801747606508641 1101 86-87 0.7410474802335827 1101 88-89 0.6672227101990345 1101 90-91 0.7412941839000204 1101 92-93 0.5228990090078938 1101 94-95 0.622909878325185 1101 96-97 0.5653629954629231 1101 98-99 0.6616609191389742 1101 100-101 0.7106482903873292 1101 102-103 0.6886758043929433 1101 104-105 0.7596570805880134 1101 106-107 0.697726073890859 1101 108-109 0.6927246788773544 1101 110-111 0.5633798903050504 1101 112-113 0.5555563919560207 1101 114-115 0.7256335721815539 1101 116-117 0.8569777669291376 1101 118-119 0.7763313886099255 1101 120-121 0.9067148394750149 1101 122-123 1.015084262483139 1101 124-125 1.1839213832868474 1102 1 -0.43982989305942155 1102 2 -0.17913360953490454 1102 3 0.09103333338969932 1102 4 0.09815000969243926 1102 5 0.16197184177419643 1102 6 -0.8897007134361559 1102 7 -1.4129051626563403 1102 8 -0.4263984360932227 1102 9 -0.2556154676848905 1102 10-11 -0.12121877551071591 1102 12-13 0.06128610491907338 1102 14-15 0.2643840251698961 1102 16-17 0.0457111664100367 1102 18-19 0.34706848177431837 1102 20-21 0.6708198664057932 1102 22-23 0.5157419670674415 1102 24-25 0.6413940068116482 1102 26-27 0.18833700838440137 1102 28-29 0.7056747427007188 1102 30-31 0.523774661395052 1102 32-33 0.3434977637729766 1102 34-35 0.7937724162726276 1102 36-37 0.9186352306966157 1102 38-39 0.8916059607138642 1102 40-41 0.7933209927092122 1102 42-43 0.5159843989583166 1102 44-45 0.6162442469457261 1102 46-47 0.2610370588670925 1102 48-49 -0.04356803653417174 1102 50-51 0.3694642777177535 1102 52-53 0.20123695556106824 1102 54-55 0.055897194420417406 1102 56-57 0.09698895112479278 1102 58-59 0.25085440476356524 1102 60-61 0.0417829798201339 1102 62-63 -0.07676559441548747 1102 64-65 0.020038006664155006 1102 66-67 0.028199152712851117 1102 68-69 -0.023834888178129177 1102 70-71 -0.0163125257308252 1102 72-73 0.09511405573309517 1102 74-75 -0.29746851743516345 1102 76-77 -0.05807185976690832 1102 78-79 -0.20200552542666017 1102 80-81 -0.4666646080071466 1102 82-83 -0.31345839623868343 1102 84-85 -0.66054010832244 1102 86-87 -0.17468301859699054 1102 88-89 0.021057242398704545 1102 90-91 -0.5675497392028142 1102 92-93 -0.01796603215361614 1102 94-95 -0.1896290339579778 1102 96-97 0.04273931826407207 1102 98-99 0.15244016926951076 1102 100-101 0.059640489313412814 1102 102-103 -0.110706318340835 1102 104-105 0.20684418253538084 1102 106-107 0.08341888787996865 1102 108-109 -0.3670673184625386 1102 110-111 -0.10940634173901387 1102 112-113 -0.35328430559454915 1102 114-115 -0.33249661630326344 1102 116-117 -0.5261351464502795 1102 118-119 -0.1375007969463553 1102 120-121 0.21678566307757308 1102 122-123 0.10479888553514627 1102 124-125 0.39531108974860274 1103 1 0.6023872179074168 1103 2 0.5409324900600865 1103 3 0.4886716292833455 1103 4 0.6056252757855134 1103 5 0.6825774439980563 1103 6 0.8514850155391969 1103 7 0.8037353012969675 1103 8 0.9253693545354196 1103 9 1.186979323771041 1103 10-11 1.2548047059990708 1103 12-13 1.1872732243792008 1103 14-15 1.3803413894546068 1103 16-17 1.3621692581561184 1103 18-19 1.3949517366758108 1103 20-21 1.7955220281627078 1103 22-23 1.767396550514821 1103 24-25 1.8106700361992374 1103 26-27 1.7478054877763753 1103 28-29 1.657694797803547 1103 30-31 1.4987372415403186 1103 32-33 1.5782504401300166 1103 34-35 2.0227341056578645 1103 36-37 1.930791449554718 1103 38-39 1.9254782567547224 1103 40-41 1.6659428595676964 1103 42-43 1.5551167409519877 1103 44-45 1.1769674925209053 1103 46-47 1.2635060420387347 1103 48-49 1.332574888799332 1103 50-51 1.3300250620625889 1103 52-53 1.3657115415736207 1103 54-55 1.2968986339168325 1103 56-57 1.1925376742879479 1103 58-59 1.2029942342542022 1103 60-61 0.99535216842634 1103 62-63 0.9754967531448813 1103 64-65 1.214340106539467 1103 66-67 1.1814111998786139 1103 68-69 1.2017438634267599 1103 70-71 1.188336174998046 1103 72-73 1.1876057222124423 1103 74-75 1.1402961266641185 1103 76-77 1.110649593269411 1103 78-79 1.1413837327390475 1103 80-81 1.0745629042688982 1103 82-83 1.1478411988423467 1103 84-85 1.033123947769436 1103 86-87 1.0290040161065086 1103 88-89 1.009998275935974 1103 90-91 1.033152588276895 1103 92-93 1.0613984385537734 1103 94-95 1.0811870274017679 1103 96-97 1.2463979795500357 1103 98-99 1.1327284674330294 1103 100-101 1.10635580619876 1103 102-103 1.0176945249942655 1103 104-105 1.134533398521107 1103 106-107 1.1902446947097793 1103 108-109 1.3035078828840483 1103 110-111 1.2831475479204535 1103 112-113 1.3603293482889818 1103 114-115 1.4529680620424514 1103 116-117 1.495021230637839 1103 118-119 1.5585711981489574 1103 120-121 1.543948852430411 1103 122-123 1.447044116487053 1103 124-125 1.514481867530641 1104 1 0.5724129029680967 1104 2 0.695148362147755 1104 3 0.5956263133862336 1104 4 0.5425373664932636 1104 5 0.5654100218323208 1104 6 0.6788369778684675 1104 7 0.7197957738250871 1104 8 0.8812226297318304 1104 9 1.0437881539796265 1104 10-11 1.0064840938029072 1104 12-13 1.0390902460255127 1104 14-15 1.0555936227854588 1104 16-17 1.0637390799526756 1104 18-19 1.4233830230254796 1104 20-21 1.6694058569796049 1104 22-23 1.3708256387810067 1104 24-25 1.8343202178467735 1104 26-27 1.8022493622509792 1104 28-29 1.722597361575282 1104 30-31 1.6338416752177665 1104 32-33 1.7816667040748406 1104 34-35 2.024307127031559 1104 36-37 1.9865217621403986 1104 38-39 2.04518926470476 1104 40-41 1.7943965949829561 1104 42-43 1.7907146638801592 1104 44-45 1.6909845798235779 1104 46-47 1.654087423413241 1104 48-49 1.6301794720505107 1104 50-51 1.480378238150145 1104 52-53 1.6399200884505483 1104 54-55 1.365069344623553 1104 56-57 1.4508455974294243 1104 58-59 1.3708838764166167 1104 60-61 1.3627469946841018 1104 62-63 1.5252633140780958 1104 64-65 1.535587587847953 1104 66-67 1.3770056422911665 1104 68-69 1.408935420288966 1104 70-71 1.445746785836338 1104 72-73 1.2932571639210906 1104 74-75 1.4285282640492767 1104 76-77 1.4222519623091578 1104 78-79 1.3780195152489618 1104 80-81 1.3387082347852193 1104 82-83 1.3947496529575538 1104 84-85 1.284705501348828 1104 86-87 1.3397492886662583 1104 88-89 1.3891865354128328 1104 90-91 1.3682576462620766 1104 92-93 1.413780978955593 1104 94-95 1.4819189761939704 1104 96-97 1.3148558718142915 1104 98-99 1.3976914589908276 1104 100-101 1.4852835732737084 1104 102-103 1.3919358740400334 1104 104-105 1.5120378480143515 1104 106-107 1.509535569567813 1104 108-109 1.541164850756651 1104 110-111 1.5541442656225435 1104 112-113 1.5884131033335187 1104 114-115 1.5177124418421428 1104 116-117 1.6138478026317067 1104 118-119 1.6211981862439302 1104 120-121 1.7044144871510767 1104 122-123 1.6113707303615605 1104 124-125 1.7889746305033647 1105 1 -0.05580929685064007 1105 2 0.19206800795205936 1105 3 0.28198625813719147 1105 4 0.4432471703325511 1105 5 0.2891807229516843 1105 6 0.353583927938093 1105 7 0.4881427701573422 1105 8 0.13417343321237496 1105 9 0.09547313208419794 1105 10-11 0.35424665085716356 1105 12-13 0.3174640787768972 1105 14-15 0.4784615117612887 1105 16-17 0.3689742798773459 1105 18-19 0.43396846534278666 1105 20-21 0.7843156269407245 1105 22-23 1.021834640035319 1105 24-25 1.114610828017078 1105 26-27 0.780432054602958 1105 28-29 0.5383001978736388 1105 30-31 0.6235053295253827 1105 32-33 0.7027372591905738 1105 34-35 1.3205925551732598 1105 36-37 1.3691468951326655 1105 38-39 1.3793825166042666 1105 40-41 0.8683161911470485 1105 42-43 0.48773479272977127 1105 44-45 0.6946772229908085 1105 46-47 0.678639650800207 1105 48-49 0.5774583185143634 1105 50-51 0.6055174738239586 1105 52-53 0.46966693361044776 1105 54-55 0.36963197086705435 1105 56-57 0.4207264762760943 1105 58-59 0.39027377775541794 1105 60-61 0.3373273685823577 1105 62-63 0.35347133829023747 1105 64-65 0.4602564441426118 1105 66-67 0.3346579366668294 1105 68-69 0.19214940388517832 1105 70-71 0.37249966100246823 1105 72-73 0.3682833273827235 1105 74-75 0.4413578788872954 1105 76-77 0.4995597843269941 1105 78-79 0.5039609520823056 1105 80-81 0.428893827763126 1105 82-83 0.33030010404377563 1105 84-85 0.3803507754728166 1105 86-87 0.38168618316443315 1105 88-89 0.6279683595217023 1105 90-91 0.44649363787134533 1105 92-93 0.3523423647055779 1105 94-95 0.4590105306541403 1105 96-97 0.6282860308523155 1105 98-99 0.4791057802428256 1105 100-101 0.5226877535935621 1105 102-103 0.5269028223594567 1105 104-105 0.5658516747065612 1105 106-107 0.5447454478674167 1105 108-109 0.5798302762474634 1105 110-111 0.64184759218195 1105 112-113 0.6224038027026779 1105 114-115 0.588491347925423 1105 116-117 0.6039797637117346 1105 118-119 0.6345444430905687 1105 120-121 0.5360711754530314 1105 122-123 0.6530711685771529 1105 124-125 0.6747494348723215 1106 1 0.21435378677580985 1106 2 0.4180747370882507 1106 3 0.2058622263848875 1106 4 0.4375654684131547 1106 5 0.14747099869425995 1106 6 0.26365877778113855 1106 7 -0.03825146685574765 1106 8 0.2686429886108215 1106 9 0.4568911991926541 1106 10-11 0.5891026492636193 1106 12-13 0.5910785903925309 1106 14-15 0.7881069062451012 1106 16-17 0.6295391886473141 1106 18-19 0.5710183442191692 1106 20-21 1.083131096019649 1106 22-23 1.1555627543696687 1106 24-25 1.2831199614601871 1106 26-27 1.4487808313191621 1106 28-29 1.273761135768396 1106 30-31 1.161411634426159 1106 32-33 0.9739874486745954 1106 34-35 1.1798500868238797 1106 36-37 1.169603410689568 1106 38-39 1.3438015930413911 1106 40-41 1.1227243829537379 1106 42-43 1.0024699681888443 1106 44-45 0.9118468975292906 1106 46-47 0.634200427461284 1106 48-49 0.7323256984900688 1106 50-51 0.6453024371235614 1106 52-53 0.6498312658478724 1106 54-55 0.45207688686977576 1106 56-57 0.5584549480723098 1106 58-59 0.5427550162271686 1106 60-61 0.5867588121144465 1106 62-63 0.5425709645003778 1106 64-65 0.5569245111466685 1106 66-67 0.39787685366593806 1106 68-69 0.3645523557017434 1106 70-71 0.6354476837079801 1106 72-73 0.65841259828996 1106 74-75 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-3.0937209544099 2216 124-125 -3.2029821617959477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 347482.0 3 50236.0 4 30105.0 5 23230.0 6 21126.0 7 21657.0 8 22834.0 9 23450.0 10 24261.0 11 25715.0 12 27059.0 13 28800.0 14 31297.0 15 27811.0 16 22377.0 17 19726.0 18 18260.0 19 17170.0 20 16933.0 21 17931.0 22 21787.0 23 28427.0 24 37578.0 25 46788.0 26 55720.0 27 61666.0 28 69156.0 29 79021.0 30 89181.0 31 99096.0 32 117327.0 33 142248.0 34 178536.0 35 233194.0 36 329227.0 37 370991.0 38 102285.0 39 207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86328652701868 18.672934364892 12.99717384041679 23.466605267672527 2 14.923392762020645 20.06838084086349 41.23147324389514 23.776753153220724 3 19.204120767118873 25.745279474159776 28.352984972521046 26.697614786200308 4 11.971424295113744 16.577124503715556 37.950632177741106 33.50081902342959 5 13.427426972722778 38.56825140164586 33.98498057977296 14.019341045858408 6 31.396402261911582 37.08500224836928 17.828544046335505 13.69005144338363 7 28.44512446039843 32.73089182757336 20.70594175515189 18.118041956876326 8 25.62593427885392 36.26319709572745 19.733323610756642 18.37754501466199 9 26.64591998522129 16.685811395025656 19.4884537842322 37.17981483552086 10-11 24.62546377558904 27.450670944600407 27.303495439937915 20.620369839872634 12-13 25.437349750276844 24.806279210039346 27.588601109317384 22.167769930366426 14-15 22.47388623162937 26.072369764569743 25.400024450183444 26.053719553617444 16-17 22.118842055683917 29.585486306879837 25.91133477181375 22.384336865622494 18-19 21.97158149861349 28.16073224078062 28.175108695897517 21.692577564708373 20-21 22.755886630948076 27.196338291650747 28.497108752675466 21.550666324725718 22-23 22.391752627436738 27.15316298537025 28.28087777473171 22.174206612461305 24-25 22.13873086304251 27.168250340028326 28.48778424466829 22.205234552260876 26-27 22.064854627825735 27.494111883844656 28.139890144067177 22.30114334426243 28-29 21.878194031112717 27.457690562830063 28.342778449197688 22.32133695685954 30-31 22.24948252495183 27.307768567032507 28.083903010192547 22.358845897823116 32-33 22.056053054056616 27.415858084496303 27.937157876934844 22.59093098451224 34-35 22.3194632475979 27.071279853605407 28.40137108630339 22.207885812493306 36-37 22.2581846626944 27.059390407694266 28.13311813748514 22.549306792126195 38-39 22.059293828420827 27.2743797950967 28.359037395460597 22.307288981021877 40-41 22.45174403621099 27.166282406535874 28.06360332962156 22.31837022763158 42-43 22.218645341512236 27.219385026552263 27.8435680739435 22.718401557992003 44-45 22.366839717975626 27.430522333412576 27.676146720328376 22.526491228283426 46-47 22.441928663226353 27.588334096702628 27.217003261016394 22.75273397905463 48-49 22.512895339269594 27.64011904939703 27.082886874939433 22.764098736393944 50-51 22.472810625763195 27.676950352208717 27.06382538378692 22.78641363824116 52-53 22.659055278057014 27.571161448594477 27.022391441354632 22.747391831993873 54-55 22.553368686133744 27.526162959299594 27.080351620170756 22.840116734395906 56-57 22.494893242064233 27.878010773457877 26.944201127697166 22.682894856780724 58-59 22.629082321632758 27.54083753980625 27.01954972538853 22.81053041317246 60-61 22.657225150606987 27.56533652850312 27.18804860190776 22.58938971898213 62-63 22.563608993725065 27.560771275764363 27.261438920986404 22.61418080952417 64-65 22.401158031499264 27.409824860933075 27.201393777595023 22.987623329972642 66-67 22.422897752418244 27.70454907297431 26.9551988946564 22.917354279951045 68-69 22.367608982184628 27.594273518670626 27.256269416915142 22.781848082229605 70-71 22.36044881394188 27.48354276537101 27.264570176009723 22.89143824467739 72-73 22.41191749835607 27.51097791810158 27.104540132497295 22.97256445104506 74-75 22.305570168356834 27.745803232409514 26.97478553905611 22.97384106017754 76-77 22.45992916655236 27.51531142165903 27.133554628534164 22.891204783254445 78-79 22.369590471482905 27.56711653220703 27.066293662488167 22.9969993338219 80-81 22.34143605832712 27.84066907598377 27.05208795425365 22.76580691143546 82-83 22.58263283339954 27.673960570141205 26.937880054780667 22.80552654167859 84-85 22.463249528194602 27.62100701588079 26.85137826205469 23.064365193869914 86-87 22.436430896679035 27.50864189771195 27.097855503685164 22.95707170192385 88-89 22.544492155266543 27.46234958588365 26.978496094998093 23.014662163851714 90-91 22.498319854543684 27.626019592145777 27.116679372676234 22.758981180634304 92-93 22.487001921172045 27.523059707388047 27.042931640826772 22.947006730613133 94-95 22.438623610066962 27.357539515699216 27.0976181954302 23.10621867880362 96-97 22.498253076738205 27.419592907838858 27.00647855000507 23.075675465417866 98-99 22.24288291291432 27.339423896606263 26.97423316717971 23.443460023299703 100-101 22.204777839359807 27.326203245697013 27.213792892693085 23.2552260222501 102-103 22.314615637028435 27.1867203491794 27.251462292896093 23.247201720896076 104-105 22.314634298364727 27.19809282031436 27.29584081418002 23.19143206714089 106-107 22.125098679830113 27.26040832398548 27.37850292779822 23.235990068386187 108-109 22.179593353229894 27.29007481175529 27.01127992513616 23.51905190987866 110-111 22.00616138281098 27.428362292364977 27.18144168162971 23.384034643194333 112-113 22.038147224117548 27.381362862187686 27.361101706833065 23.21938820686171 114-115 21.95908184152547 27.43054868319852 27.176008153075028 23.434361322200985 116-117 21.77018606581143 27.366397038780928 27.34424345332035 23.5191734420873 118-119 21.862863055771133 27.311117245594023 27.178647138176913 23.64737256045793 120-121 21.849255241815282 27.29334355824493 27.34237313207133 23.515028067868457 122-123 21.89560730512744 27.302193309131063 27.263546066783682 23.53865331895781 124-125 21.908089531347954 27.258402029719907 27.07602593067902 23.757482508253116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1381.0 1 1434.0 2 1428.5 3 1162.0 4 835.0 5 726.5 6 718.0 7 865.0 8 1186.0 9 1379.0 10 1368.5 11 1384.0 12 1535.0 13 1751.0 14 1923.5 15 2133.0 16 2389.5 17 2586.5 18 2901.5 19 3275.5 20 3796.5 21 4513.5 22 5390.0 23 6711.0 24 8555.0 25 10870.0 26 14153.0 27 18359.5 28 22492.5 29 27173.5 30 33412.0 31 40166.5 32 46734.5 33 53565.0 34 60388.5 35 66217.5 36 72492.5 37 79262.0 38 82969.5 39 85193.5 40 87536.0 41 90045.5 42 92328.0 43 96138.5 44 101142.0 45 103832.5 46 103742.0 47 103491.5 48 105925.0 49 107392.5 50 106399.0 51 103619.5 52 101145.5 53 99906.5 54 96536.0 55 89928.5 56 81977.0 57 73438.0 58 66318.0 59 59564.0 60 51307.5 61 42317.5 62 33732.5 63 26468.5 64 20526.5 65 15888.0 66 11858.5 67 8996.5 68 6540.0 69 4531.0 70 3346.5 71 2504.5 72 1886.0 73 1431.5 74 1109.0 75 873.0 76 614.5 77 389.0 78 265.5 79 192.5 80 141.0 81 105.0 82 83.5 83 58.5 84 36.5 85 33.0 86 31.5 87 22.5 88 18.5 89 16.0 90 12.5 91 14.5 92 12.0 93 7.5 94 6.5 95 3.0 96 1.5 97 2.5 98 3.0 99 3.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012812967139426958 2 0.004896011833764772 3 0.008229466699306746 4 0.008541978092951305 5 0.014688035501294317 6 0.0010417046454818666 7 0.00777806135293127 8 0.0 9 0.002673708590070124 10-11 0.0 12-13 0.003680689747369262 14-15 0.019827111752338193 16-17 0.03232756749812059 18-19 0.006475930546078938 20-21 0.00887185123068723 22-23 0.0015451952241314353 24-25 0.012344200048960117 26-27 0.02008753791370866 28-29 3.993201141013822E-4 30-31 0.0015972804564055288 32-33 0.009236447856605884 34-35 0.009739938435255453 36-37 0.012066412143498288 38-39 0.0 40-41 0.02670236241251851 42-43 0.010104535061174105 44-45 0.01730965885909035 46-47 0.004097371605562008 48-49 0.02618151008977758 50-51 0.0048439266014906794 52-53 0.0 54-55 0.01868123664230814 56-57 0.004600862184211577 58-59 2.777879054618311E-4 60-61 0.004566138696028848 62-63 0.028507983798020414 64-65 0.018264554784115393 66-67 0.002309111964151471 68-69 0.022223032436946486 70-71 0.0028646877750751333 72-73 0.012656711442604678 74-75 0.0 76-77 0.006319674849256657 78-79 0.0011979603423041465 80-81 0.0035591575387297104 82-83 0.0016320039445882574 84-85 0.0 86-87 0.0060592486878861904 88-89 0.01774370246137446 90-91 0.023611971964255643 92-93 0.0049480970660388665 94-95 0.02619887183386894 96-97 0.0189937480359527 98-99 0.0010417046454818666 100-101 0.009271171344788613 102-103 0.0 104-105 0.0014063012714005199 106-107 1.7361744091364444E-5 108-109 0.0 110-111 7.639167400200355E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.7361744091364444E-5 122-123 0.0 124-125 0.023611971964255643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2879895.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.74367176782927 #Duplication Level Percentage of deduplicated Percentage of total 1 81.62320100399346 50.39716131430124 2 9.665236156351206 11.935343375926093 3 3.59727116702568 6.663261905901291 4 1.6878247701535103 4.168499944398809 5 0.9298344084703262 2.8705695257512756 6 0.5627754792419223 2.0848694681577635 7 0.3684620776622352 1.5925141107448624 8 0.2731247257742787 1.3490978735908357 9 0.2022347692893463 1.123804549353967 >10 0.9880762345996555 10.927874124805925 >50 0.07023279767940843 2.961326622814653 >100 0.031040050457069004 3.3239400226154014 >500 4.575728679528666E-4 0.1860409277491698 >1k 2.2878643392128798E-4 0.41569623388869065 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4841 0.1680964062925905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3318 0.11521253379029443 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.472348818272889E-5 2 0.0 0.0 0.0 0.0 3.472348818272889E-5 3 0.0 0.0 0.0 0.0 3.472348818272889E-5 4 0.0 3.472348818272889E-5 0.0 0.0 3.472348818272889E-5 5 0.0 3.472348818272889E-5 0.0 0.0 3.472348818272889E-5 6 0.0 3.472348818272889E-5 0.0 0.0 3.472348818272889E-5 7 0.0 3.472348818272889E-5 0.0 0.0 3.472348818272889E-5 8 0.0 3.472348818272889E-5 0.0 0.0 3.472348818272889E-5 9 0.0 3.472348818272889E-5 0.0 0.0 3.472348818272889E-5 10-11 0.0 3.472348818272889E-5 0.0 5.208523227409333E-5 3.472348818272889E-5 12-13 0.0 3.472348818272889E-5 0.0 1.0417046454818665E-4 3.472348818272889E-5 14-15 0.0 3.472348818272889E-5 0.0 1.0417046454818665E-4 3.472348818272889E-5 16-17 0.0 3.472348818272889E-5 0.0 1.3889395273091555E-4 3.472348818272889E-5 18-19 0.0 3.472348818272889E-5 0.0 2.083409290963733E-4 3.472348818272889E-5 20-21 0.0 3.472348818272889E-5 0.0 2.6042616137046665E-4 6.944697636545777E-5 22-23 0.0 3.472348818272889E-5 0.0 3.993201141013822E-4 6.944697636545777E-5 24-25 0.0 3.472348818272889E-5 0.0 5.729375550150266E-4 6.944697636545777E-5 26-27 0.0 3.472348818272889E-5 0.0 7.812784841113999E-4 6.944697636545777E-5 28-29 0.0 3.472348818272889E-5 0.0 0.0014063012714005197 6.944697636545777E-5 30-31 0.0 3.472348818272889E-5 0.0 0.0029167730073492264 6.944697636545777E-5 32-33 0.0 3.472348818272889E-5 0.0 0.006510654034261666 6.944697636545777E-5 34-35 0.0 3.472348818272889E-5 0.0 0.011302495403478252 6.944697636545777E-5 36-37 0.0 3.472348818272889E-5 0.0 0.020139623145982753 6.944697636545777E-5 38-39 0.0 3.472348818272889E-5 0.0 0.0369110679382408 6.944697636545777E-5 40-41 0.0 3.472348818272889E-5 0.0 0.05725903201331993 6.944697636545777E-5 42-43 0.0 3.472348818272889E-5 0.0 0.0772944846947545 6.944697636545777E-5 44-45 0.0 6.944697636545777E-5 0.0 0.09920500573805642 6.944697636545777E-5 46-47 0.0 6.944697636545777E-5 0.0 0.12707060500469636 6.944697636545777E-5 48-49 0.0 6.944697636545777E-5 0.0 0.15875578797143647 6.944697636545777E-5 50-51 0.0 6.944697636545777E-5 0.0 0.1958404733505909 6.944697636545777E-5 52-53 0.0 6.944697636545777E-5 0.0 0.2326126473361008 1.0417046454818665E-4 54-55 0.0 6.944697636545777E-5 0.0 0.2722321473525945 1.3889395273091555E-4 56-57 0.0 6.944697636545777E-5 0.0 0.3198727731392985 1.3889395273091555E-4 58-59 0.0 6.944697636545777E-5 0.0 0.37664567631806023 1.3889395273091555E-4 60-61 0.0 6.944697636545777E-5 0.0 0.44053689457428136 1.3889395273091555E-4 62-63 0.0 6.944697636545777E-5 0.0 0.5046711772477817 1.3889395273091555E-4 64-65 0.0 6.944697636545777E-5 0.0 0.5721041912986411 1.3889395273091555E-4 66-67 0.0 6.944697636545777E-5 0.0 0.6485479505329188 1.3889395273091555E-4 68-69 0.0 8.680872045682221E-5 0.0 0.7410165995635257 1.3889395273091555E-4 70-71 0.0 1.0417046454818665E-4 0.0 0.8443016151630528 1.3889395273091555E-4 72-73 0.0 1.0417046454818665E-4 0.0 0.9540799230527501 1.7361744091364442E-4 74-75 0.0 1.0417046454818665E-4 0.0 1.0624345679269558 2.083409290963733E-4 76-77 0.0 1.0417046454818665E-4 0.0 1.1790013177563765 2.083409290963733E-4 78-79 1.7361744091364444E-5 1.0417046454818665E-4 0.0 1.3202564676837176 2.083409290963733E-4 80-81 3.472348818272889E-5 1.0417046454818665E-4 0.0 1.481060941457935 2.083409290963733E-4 82-83 3.472348818272889E-5 1.0417046454818665E-4 0.0 1.6562930245720764 2.777879054618311E-4 84-85 6.944697636545777E-5 1.0417046454818665E-4 0.0 1.8323758331466946 2.777879054618311E-4 86-87 6.944697636545777E-5 1.0417046454818665E-4 0.0 2.0162540648183356 3.1251139364456E-4 88-89 6.944697636545777E-5 1.0417046454818665E-4 0.0 2.2295778144689304 3.4723488182728885E-4 90-91 6.944697636545777E-5 1.0417046454818665E-4 0.0 2.473006828373951 3.4723488182728885E-4 92-93 6.944697636545777E-5 1.0417046454818665E-4 0.0 2.7364365714722236 3.4723488182728885E-4 94-95 6.944697636545777E-5 1.0417046454818665E-4 0.0 3.0010989984009835 3.645966259186533E-4 96-97 6.944697636545777E-5 1.0417046454818665E-4 0.0 3.272827655174928 4.166818581927466E-4 98-99 6.944697636545777E-5 1.0417046454818665E-4 0.0 3.5659286189253425 4.514053463754755E-4 100-101 6.944697636545777E-5 1.0417046454818665E-4 0.0 3.901826281860971 4.514053463754755E-4 102-103 6.944697636545777E-5 1.0417046454818665E-4 0.0 4.270138321015176 4.514053463754755E-4 104-105 6.944697636545777E-5 1.0417046454818665E-4 0.0 4.643172754562232 4.514053463754755E-4 106-107 6.944697636545777E-5 1.0417046454818665E-4 0.0 5.019176046348912 4.514053463754755E-4 108-109 6.944697636545777E-5 1.0417046454818665E-4 0.0 5.414763385470652 4.514053463754755E-4 110-111 6.944697636545777E-5 1.0417046454818665E-4 0.0 5.853355764706699 4.514053463754755E-4 112-113 6.944697636545777E-5 1.0417046454818665E-4 0.0 6.33293922174246 4.514053463754755E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3810 0.0 60.123466 1 GTATCAA 7300 0.0 54.608498 1 ATCAACG 8875 0.0 44.04588 3 TCAACGC 8970 0.0 43.181408 4 CAACGCA 9065 0.0 42.92578 5 AACGCAG 9370 0.0 41.39505 6 TATCAAC 10040 0.0 39.76395 2 GTACATG 12540 0.0 37.483303 1 TACATGG 12620 0.0 36.160694 2 ACGCAGA 11130 0.0 34.261852 7 ACATGGG 12860 0.0 34.14473 3 CGCAGAG 11095 0.0 33.677563 8 GCAGAGT 12235 0.0 28.984491 9 CATGGGA 8255 0.0 27.244762 4 CATGGGG 5965 0.0 25.235874 4 GAGTACT 6010 0.0 24.160955 12-13 CAGAGTA 11730 0.0 24.071495 10-11 AGAGTAC 11220 0.0 23.441978 10-11 GAGTACA 9280 0.0 22.247938 1 GTGGTAT 2530 0.0 22.106277 1 >>END_MODULE