FastQCFastQC Report
Thu 26 May 2016
SRR1364574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879895
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76250.26476659739330777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66230.22997366223421342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42290.14684563152476043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53350.066.2583541
GTATCAA97950.051.7899481
ATCAACG125400.040.1295433
TCAACGC127150.039.483554
CAACGCA128350.038.985025
AACGCAG133500.037.570246
TATCAAC135100.036.808132
GTACATG134900.032.831991
ACGCAGA157250.031.5553537
TACATGG136950.031.1795392
CGCAGAG158750.031.1072778
ACATGGG135750.030.182393
GCAGAGT174500.027.6503379
GTGGTAT30400.027.059881
TGGTATC28200.026.1869642
TATCACG1608.685678E-626.0549742
GAGTACT102400.025.70963512-13
CATGGGA84400.025.1901534
GTACTTT107250.024.46379714-15
CAGAGTA168800.024.28408810-11
Produced by FastQC (version 0.11.5)