FastQCFastQC Report
Thu 26 May 2016
SRR1364573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525980
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.054.801131
GTATCAA78600.050.2649961
ATCAACG87650.044.3943253
CAACGCA87400.044.3851625
TCAACGC87700.044.3690154
AACGCAG89650.043.1323476
TATCAAC98250.039.544152
GTACATG142850.036.1126171
ACGCAGA108900.035.0709237
TACATGG149900.033.6188932
CGCAGAG113100.032.8776368
ACATGGG147650.032.5999373
GCAGAGT129300.027.4243939
CATGGGG87700.026.5942734
GAGTACA106600.023.4429471
AGTACAT99350.023.3559952
CATGGGA73600.023.120114
CAGAGTA124600.022.51784910-11
AGAGTAC115050.022.26638810-11
GAGTACT53800.021.68119612-13
Produced by FastQC (version 0.11.5)