##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364573_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3525980 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.576175134288906 31.0 28.0 33.0 10.0 34.0 2 27.60579696992042 31.0 27.0 34.0 10.0 34.0 3 27.706736283246077 31.0 27.0 34.0 10.0 34.0 4 30.960983329457342 35.0 32.0 37.0 12.0 37.0 5 31.082573355492656 35.0 32.0 37.0 12.0 37.0 6 31.030314125434632 35.0 32.0 37.0 10.0 37.0 7 30.831843062070686 35.0 31.0 37.0 10.0 37.0 8 30.763020209984173 35.0 31.0 37.0 10.0 37.0 9 31.777305032927018 37.0 31.0 39.0 8.0 39.0 10-11 31.684996937021765 37.0 31.0 39.0 8.0 39.0 12-13 31.606814417552 37.0 31.0 39.0 7.5 39.0 14-15 32.253886579050366 38.0 31.0 40.0 4.5 41.0 16-17 32.34476939744411 38.0 31.0 40.0 2.0 41.0 18-19 32.12490867787112 38.0 30.5 40.0 2.0 41.0 20-21 31.82021338748376 38.0 30.0 40.0 2.0 41.0 22-23 31.701547796640934 38.0 30.0 40.0 2.0 41.0 24-25 31.508730338799424 37.5 29.0 40.0 2.0 41.0 26-27 31.388604728330847 37.0 29.5 40.0 2.0 41.0 28-29 31.435349321323432 37.0 29.5 40.0 2.0 41.0 30-31 31.379593191112825 37.0 29.5 40.0 2.0 41.0 32-33 31.36736807922904 37.0 30.0 40.0 2.0 41.0 34-35 30.88251890254624 37.0 28.5 40.0 2.0 41.0 36-37 30.753068366808662 37.0 28.5 40.0 2.0 41.0 38-39 30.54697530899211 37.0 27.5 40.0 2.0 41.0 40-41 30.76889857571512 37.0 28.5 40.0 2.0 41.0 42-43 30.64882500751564 37.0 28.5 40.0 2.0 41.0 44-45 30.494261028139697 36.5 28.0 40.0 2.0 41.0 46-47 30.468319587745818 36.0 28.0 40.0 2.0 41.0 48-49 30.329309298407818 36.0 28.0 40.0 2.0 41.0 50-51 30.16822500411233 36.0 28.0 40.0 2.0 41.0 52-53 30.106020453888 36.0 28.0 40.0 2.0 41.0 54-55 30.120592005626804 36.0 28.0 40.0 2.0 41.0 56-57 29.978796107748767 36.0 28.0 40.0 2.0 41.0 58-59 29.767687848484677 35.5 28.0 40.0 2.0 41.0 60-61 29.55469883550105 35.0 27.0 39.0 2.0 41.0 62-63 29.263259717865672 35.0 26.5 39.0 2.0 41.0 64-65 28.975348980992518 35.0 26.0 39.0 2.0 40.5 66-67 28.723980708909295 34.0 26.0 38.5 2.0 40.0 68-69 28.68807749902155 34.0 26.0 38.0 2.0 40.0 70-71 28.466639062047996 34.0 26.0 37.0 2.0 40.0 72-73 28.194092280727627 34.0 26.0 37.0 2.0 39.5 74-75 27.898360172207443 34.0 26.0 36.5 2.0 39.0 76-77 27.601344307114616 34.0 26.0 36.0 2.0 39.0 78-79 27.30809080028815 34.0 26.0 35.5 2.0 37.5 80-81 27.02212221850379 34.0 25.0 35.0 2.0 37.0 82-83 26.789046165888635 34.0 25.0 35.0 2.0 37.0 84-85 26.556603837798285 33.0 25.0 35.0 2.0 36.0 86-87 26.37530700684632 33.0 24.0 35.0 2.0 36.0 88-89 26.188402373240915 33.0 24.0 35.0 2.0 36.0 90-91 26.059556633900364 33.0 23.5 35.0 2.0 35.0 92-93 25.943104895660213 33.0 23.0 35.0 2.0 35.0 94-95 25.81852463712216 33.0 23.0 35.0 2.0 35.0 96-97 25.70301504829863 33.0 21.5 35.0 2.0 35.0 98-99 25.610241691671536 33.0 20.0 35.0 2.0 35.0 100-101 25.517185293166722 33.0 20.0 35.0 2.0 35.0 102-103 24.394512873016865 31.0 18.5 34.0 2.0 35.0 104-105 25.23781516060783 32.5 19.0 35.0 2.0 35.0 106-107 25.378818654671893 33.0 19.0 35.0 2.0 35.0 108-109 25.330446145468777 33.0 19.0 35.0 2.0 35.0 110-111 25.239183432691053 33.0 18.0 35.0 2.0 35.0 112-113 25.141684581307892 33.0 17.5 35.0 2.0 35.0 114-115 25.009111651228878 33.0 16.0 35.0 2.0 35.0 116-117 24.893060227227608 33.0 8.5 35.0 2.0 35.0 118-119 24.734665965206837 33.0 2.0 35.0 2.0 35.0 120-121 24.540919971185318 33.0 2.0 35.0 2.0 35.0 122-123 24.283610230347307 33.0 2.0 35.0 2.0 35.0 124-125 22.849697956312852 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9047662662285028 1101 2 0.8451904877757066 1101 3 0.8488919715914065 1101 4 0.955615751873804 1101 5 0.9080936975322409 1101 6 1.0079567178728048 1101 7 1.1165316721910372 1101 8 1.0258793338929948 1101 9 1.336606237899442 1101 10-11 1.3797207249136818 1101 12-13 1.1350737461565963 1101 14-15 1.4113488952390156 1101 16-17 1.234427873195088 1101 18-19 1.5942026105328253 1101 20-21 1.9453194712615591 1101 22-23 1.8715459463266306 1101 24-25 2.0144318616032386 1101 26-27 2.106479269270846 1101 28-29 1.8377510838841928 1101 30-31 1.878861596305068 1101 32-33 1.8916784386698318 1101 34-35 2.1241831527924724 1101 36-37 2.010753548274728 1101 38-39 1.8213680023814192 1101 40-41 1.7529979786564915 1101 42-43 1.7078610236335408 1101 44-45 1.661469968241331 1101 46-47 1.522791155750717 1101 48-49 1.6047059776053096 1101 50-51 1.559074636179318 1101 52-53 1.353551789639937 1101 54-55 1.3148186233776862 1101 56-57 1.4023043336598917 1101 58-59 1.3684378909212391 1101 60-61 1.3837053587824641 1101 62-63 1.3087005891380556 1101 64-65 1.406568669350854 1101 66-67 1.3208777577734168 1101 68-69 1.1933758654435138 1101 70-71 1.1707684155116738 1101 72-73 1.1096388123976482 1101 74-75 1.0583012852155278 1101 76-77 1.2146295491945196 1101 78-79 1.318217999728443 1101 80-81 1.36000075251928 1101 82-83 1.128706723276487 1101 84-85 1.2794283223007916 1101 86-87 1.3193116575174635 1101 88-89 1.2752692011524474 1101 90-91 1.2534624972296307 1101 92-93 1.1185436310609944 1101 94-95 1.1896622069825433 1101 96-97 1.211890709725651 1101 98-99 1.2504296304297924 1101 100-101 1.321807756149834 1101 102-103 1.1937130030961391 1101 104-105 1.2612791434033426 1101 106-107 1.290037288415725 1101 108-109 1.2870238053167213 1101 110-111 1.0928666990419664 1101 112-113 1.0915379077946206 1101 114-115 1.294812035040458 1101 116-117 1.2815641406126161 1101 118-119 1.2579815624656128 1101 120-121 1.3499063600984194 1101 122-123 1.3894050542339187 1101 124-125 1.5361641606325414 1102 1 -0.14003741929357005 1102 2 0.16607201438063157 1102 3 0.24061340903104522 1102 4 0.32610600185126515 1102 5 0.3435210480351394 1102 6 -0.43544369860898513 1102 7 -0.939078929120754 1102 8 -0.14945792325504925 1102 9 0.12482063704431923 1102 10-11 0.17334285401478766 1102 12-13 0.3189707043310577 1102 14-15 0.6062753583316436 1102 16-17 0.46558859816095577 1102 18-19 0.5970666962262428 1102 20-21 1.048029107205327 1102 22-23 0.8651860254510915 1102 24-25 1.006738136878372 1102 26-27 0.5506511242823855 1102 28-29 1.0617581684350625 1102 30-31 0.9237227705150168 1102 32-33 0.8191365164897029 1102 34-35 1.053347882300276 1102 36-37 1.2714469229851844 1102 38-39 1.317966299861844 1102 40-41 1.0849458014667057 1102 42-43 0.9741783829928679 1102 44-45 0.9588575511561466 1102 46-47 0.6197293841003528 1102 48-49 0.3515077872505046 1102 50-51 0.6697040109911256 1102 52-53 0.5549137449005528 1102 54-55 0.4200712406781797 1102 56-57 0.44784226131533345 1102 58-59 0.56755480427735 1102 60-61 0.4126514103076495 1102 62-63 0.30434275509084685 1102 64-65 0.41731872253065205 1102 66-67 0.39352235673881 1102 68-69 0.20774078227384152 1102 70-71 0.2569089719285138 1102 72-73 0.5184529146784556 1102 74-75 0.16645621579305825 1102 76-77 0.26688577211481146 1102 78-79 0.28106981134762776 1102 80-81 -0.08125803540557541 1102 82-83 0.0020278399874378294 1102 84-85 -0.2232124745299835 1102 86-87 0.20414133926951905 1102 88-89 0.38918916806017734 1102 90-91 -0.23956317844843156 1102 92-93 0.1990600923446273 1102 94-95 0.18772718415206313 1102 96-97 0.41373404528045654 1102 98-99 0.39281633094297064 1102 100-101 0.3357764761165072 1102 102-103 0.14106201960868603 1102 104-105 0.47262472193767735 1102 106-107 0.3999681075378234 1102 108-109 -0.03472653335462539 1102 110-111 0.193997071748381 1102 112-113 -0.09694348782006301 1102 114-115 0.0018202191315346283 1102 116-117 -0.07109463570401786 1102 118-119 0.370706236065395 1102 120-121 0.739139284963759 1102 122-123 0.5999153342866919 1102 124-125 0.8700006396761601 1103 1 0.6411122808343848 1103 2 0.5779948768540919 1103 3 0.467402812360735 1103 4 0.6420581938837486 1103 5 0.6452999075920864 1103 6 0.6664770551099295 1103 7 0.6777103312425261 1103 8 0.6799313242412524 1103 9 0.888123661498728 1103 10-11 1.0238732593960478 1103 12-13 0.9628811394493262 1103 14-15 1.341151299229331 1103 16-17 1.1918391968327953 1103 18-19 1.1929859881136196 1103 20-21 1.5593792998525693 1103 22-23 1.5096745809899694 1103 24-25 1.5273177048338802 1103 26-27 1.4299021411309774 1103 28-29 1.3813256235614801 1103 30-31 1.3782746423027312 1103 32-33 1.4244089389840404 1103 34-35 1.7521958410973504 1103 36-37 1.6758086494475535 1103 38-39 1.8432217629657828 1103 40-41 1.450684705486207 1103 42-43 1.2276457652064146 1103 44-45 0.9279133652426275 1103 46-47 1.0004096182793312 1103 48-49 1.1003466319389439 1103 50-51 1.0115773534743084 1103 52-53 0.9799287563736101 1103 54-55 0.9345604395659031 1103 56-57 0.8783266315125573 1103 58-59 0.752533234220234 1103 60-61 0.6739059941681731 1103 62-63 0.7083926360713093 1103 64-65 0.8238530916556286 1103 66-67 0.9388668343038091 1103 68-69 0.9539030277042784 1103 70-71 0.9246590559212855 1103 72-73 0.9741690691679601 1103 74-75 0.8892984058954951 1103 76-77 0.8906321354067543 1103 78-79 0.9145003618397887 1103 80-81 0.9071330920990164 1103 82-83 0.9631762712885035 1103 84-85 0.9810302323960229 1103 86-87 0.9242059600868551 1103 88-89 0.868585921293004 1103 90-91 0.9073057678176433 1103 92-93 0.8990598242826096 1103 94-95 0.872249908662937 1103 96-97 1.0049314247061645 1103 98-99 0.9900871021752664 1103 100-101 0.9266392247887083 1103 102-103 0.8316219475536144 1103 104-105 0.7753426921005868 1103 106-107 0.8948651002393895 1103 108-109 1.0658851841723411 1103 110-111 1.0156299447298736 1103 112-113 1.091966071628942 1103 114-115 1.077257338930238 1103 116-117 1.1139490226634479 1103 118-119 1.2546452108532655 1103 120-121 1.1919929838622032 1103 122-123 1.1853248079558512 1103 124-125 1.2075479079454823 1104 1 0.6164740277873655 1104 2 0.6235572961176459 1104 3 0.5189292114605806 1104 4 0.6455714737844893 1104 5 0.6852244479377312 1104 6 0.6335941491083261 1104 7 0.6331066528858678 1104 8 0.5736170260586668 1104 9 0.9716840945685981 1104 10-11 0.8488626931934178 1104 12-13 0.865716317345754 1104 14-15 0.8633764685830556 1104 16-17 0.8984109121110109 1104 18-19 1.1869811317270305 1104 20-21 1.3703827132899171 1104 22-23 1.1544124508210807 1104 24-25 1.6191896096446676 1104 26-27 1.6813726452292919 1104 28-29 1.5716577094238318 1104 30-31 1.4860601807822214 1104 32-33 1.4749306373507132 1104 34-35 1.7528950544726634 1104 36-37 1.7689759482634102 1104 38-39 1.754163819509202 1104 40-41 1.3964223820827222 1104 42-43 1.4273888178756025 1104 44-45 1.314169175602359 1104 46-47 1.3088813148791303 1104 48-49 1.1771625744386078 1104 50-51 0.9813114932325675 1104 52-53 1.1102718403417526 1104 54-55 0.9812573408569527 1104 56-57 0.862887179850091 1104 58-59 0.9380289861440296 1104 60-61 0.9533550596026217 1104 62-63 1.019058029184528 1104 64-65 0.9624238609537059 1104 66-67 0.9022083864179749 1104 68-69 0.9273611403883777 1104 70-71 0.9515285516098224 1104 72-73 0.8902664790390205 1104 74-75 0.9479803748354882 1104 76-77 0.9457426297003053 1104 78-79 0.9355577800011474 1104 80-81 0.8818431280142889 1104 82-83 0.920035945386708 1104 84-85 0.9607617737238279 1104 86-87 0.9888056685802589 1104 88-89 0.9158860496860441 1104 90-91 0.955621499694395 1104 92-93 0.9407095233246245 1104 94-95 0.96877146848405 1104 96-97 0.8131570206343781 1104 98-99 0.8910655171130593 1104 100-101 0.9549959117436018 1104 102-103 0.9807973420298559 1104 104-105 0.9496247372125914 1104 106-107 0.9683912867531284 1104 108-109 1.0556171419782032 1104 110-111 1.097443518773506 1104 112-113 1.0558646806763612 1104 114-115 1.1010262466134577 1104 116-117 1.196387909766763 1104 118-119 1.1421140310901166 1104 120-121 1.135723292302771 1104 122-123 1.111055411436766 1104 124-125 1.4269410475281745 1105 1 0.09940049212845636 1105 2 0.4148954645346805 1105 3 0.3995821573796654 1105 4 0.4612121267276592 1105 5 0.5858225093086595 1105 6 0.4829062091180347 1105 7 0.5239294736910267 1105 8 0.3214570010857791 1105 9 0.27565386016392424 1105 10-11 0.5199931995603819 1105 12-13 0.46133496918502104 1105 14-15 0.6669937642877954 1105 16-17 0.5868712568256669 1105 18-19 0.6999326218770889 1105 20-21 1.1095453036607985 1105 22-23 1.3069789415838464 1105 24-25 1.306718075789309 1105 26-27 1.0794515966978615 1105 28-29 0.8167757763562022 1105 30-31 0.7783624438677883 1105 32-33 1.0150126872004144 1105 34-35 1.5948957954944767 1105 36-37 1.4836419156030516 1105 38-39 1.5725717923595752 1105 40-41 1.1862645379764736 1105 42-43 0.7469354021478658 1105 44-45 0.9363567789301257 1105 46-47 0.8825623500059159 1105 48-49 0.836553225234038 1105 50-51 0.7941652045168084 1105 52-53 0.7570629382382137 1105 54-55 0.48582749081868215 1105 56-57 0.5602431711406481 1105 58-59 0.554000686560908 1105 60-61 0.5612572029370781 1105 62-63 0.45234678803760175 1105 64-65 0.5940059706995697 1105 66-67 0.4286375286298991 1105 68-69 0.24358340129664668 1105 70-71 0.49457030731605656 1105 72-73 0.513960610224629 1105 74-75 0.5548940251761465 1105 76-77 0.6181406977927963 1105 78-79 0.5951494077055202 1105 80-81 0.5541359224878164 1105 82-83 0.507624197737421 1105 84-85 0.47416882527968696 1105 86-87 0.5007695763782323 1105 88-89 0.6777263851210904 1105 90-91 0.5511471286230822 1105 92-93 0.4614019319884619 1105 94-95 0.5594809882775884 1105 96-97 0.6889501809796954 1105 98-99 0.4829380638356824 1105 100-101 0.4624415246120108 1105 102-103 0.4341842556891322 1105 104-105 0.6372229104034517 1105 106-107 0.6384675964672581 1105 108-109 0.5871214451211415 1105 110-111 0.6577298561443392 1105 112-113 0.6474751172215072 1105 114-115 0.7551899857674584 1105 116-117 0.6837158835325852 1105 118-119 0.7627964811135826 1105 120-121 0.6080731685482803 1105 122-123 0.7662097741425455 1105 124-125 0.7957026792579285 1106 1 0.2949224085111304 1106 2 0.5729752305830402 1106 3 0.29780721812107913 1106 4 0.41854703537820726 1106 5 0.28611659683515356 1106 6 0.3146226680831923 1106 7 0.15545816727061634 1106 8 0.46441437272767416 1106 9 0.5934115826446451 1106 10-11 0.7046423700780409 1106 12-13 0.6445166795056174 1106 14-15 0.9031517363468566 1106 16-17 0.8294351465306633 1106 18-19 0.7936791316130538 1106 20-21 1.077441019885999 1106 22-23 1.0724387728989733 1106 24-25 1.1849862352543816 1106 26-27 1.533949321002435 1106 28-29 1.2734620714772475 1106 30-31 1.0223102697825404 1106 32-33 1.0575466502174784 1106 34-35 1.2590053875518983 1106 36-37 1.1990581378759444 1106 38-39 1.3740349456616983 1106 40-41 1.1181151269443568 1106 42-43 0.9495843814172318 1106 44-45 0.8883668386457089 1106 46-47 0.630202299683102 1106 48-49 0.8599031738109595 1106 50-51 0.852080494956283 1106 52-53 0.7497749726651222 1106 54-55 0.48695029555729263 1106 56-57 0.6669124602585583 1106 58-59 0.6050168783393239 1106 60-61 0.589659997876467 1106 62-63 0.5066767096223401 1106 64-65 0.48209623947820646 1106 66-67 0.3328496234018061 1106 68-69 0.48659257339026496 1106 70-71 0.5819913420939571 1106 72-73 0.5513167092136868 1106 74-75 0.622362503656106 1106 76-77 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-2.9803897076528614 2216 100-101 -3.0609488646393466 2216 102-103 -2.858334224252296 2216 104-105 -3.1231963174485777 2216 106-107 -3.053979721565966 2216 108-109 -3.1630264701210358 2216 110-111 -3.175016558485762 2216 112-113 -3.0681357991033344 2216 114-115 -3.186277436451217 2216 116-117 -3.3307301327720396 2216 118-119 -3.342437518503395 2216 120-121 -3.4750250528818647 2216 122-123 -3.4840192353906794 2216 124-125 -3.511809835668572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 295291.0 3 46113.0 4 28264.0 5 22617.0 6 20718.0 7 21920.0 8 22581.0 9 23501.0 10 24302.0 11 25725.0 12 26820.0 13 28745.0 14 30388.0 15 27320.0 16 21969.0 17 19865.0 18 18651.0 19 17844.0 20 17749.0 21 19097.0 22 23114.0 23 30954.0 24 40605.0 25 51700.0 26 62447.0 27 71055.0 28 80483.0 29 94021.0 30 106350.0 31 121769.0 32 147214.0 33 182612.0 34 233925.0 35 314057.0 36 471648.0 37 572381.0 38 161873.0 39 292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.279880540523216 18.48907417770916 12.790213830648122 24.4408314511195 2 15.223642693553991 19.676019087731238 40.625725893939716 24.47461232477505 3 19.28242904727761 24.88484647709636 28.321498425289754 27.51122605033627 4 12.533007348832726 16.133311399341412 37.121589004262944 34.212092247562914 5 13.813134930371282 37.93520607802898 34.102173176172926 14.149485815426813 6 32.420197257812376 36.63322503136749 17.275260185641063 13.671317525179072 7 29.18371063106584 32.00304848474879 20.60197646642384 18.211264417761537 8 26.420200908683544 35.71662346354773 19.40311629674587 18.460059331022865 9 26.76087455933112 16.32311274600172 19.420741997056062 37.4952706976111 10-11 25.23478579004986 26.633772738359262 27.213441369491605 20.918000102099274 12-13 25.787962812355662 23.903239177746862 27.466424039490068 22.842373970407408 14-15 22.982013075262326 25.369679540621277 24.940806170738632 26.707501213377764 16-17 22.97862911971276 28.600671826862094 25.504455275114672 22.91624377831047 18-19 22.903511400454683 27.478512779590545 27.404287616409352 22.21368820354542 20-21 23.48239687446331 26.645061295502565 27.552847099973864 22.31969473006026 22-23 23.31843830356336 26.4216916180041 27.16798408574954 23.091885992682997 24-25 23.06053170279398 26.540096966678306 27.429846936966552 22.96952439356116 26-27 22.87845063848274 26.890326035881206 27.296706083488885 22.934517242147166 28-29 22.842301924651778 26.83209194652524 27.41537948857936 22.91022664024362 30-31 23.05150688459201 26.731982063105853 27.149391208017658 23.06711984428448 32-33 22.899081782951562 26.837419734362893 27.010622477583485 23.252876005102056 34-35 23.114169037993946 26.5671004665127 27.462643174867452 22.856087320625903 36-37 23.19767559770436 26.443564928777807 27.188143472021327 23.1706160014965 38-39 22.90125664389947 26.774095931938334 27.355523104363357 22.96912431979884 40-41 23.17657248079531 26.710209299099503 27.127394752586557 22.985823467518625 42-43 23.031545364632215 26.619636654081653 26.96095241093697 23.387865570349167 44-45 23.112380453681393 26.709772435974262 26.86032445769006 23.31752265265429 46-47 23.272904820128314 26.912768352544475 26.52307166079711 23.291255166530103 48-49 23.261439120174714 26.924975961425947 26.473196865815325 23.340388052584018 50-51 23.142090834975154 27.014660312952067 26.42360489209364 23.41964395997914 52-53 23.40228815818581 26.907299530910556 26.319491318725575 23.370920992178064 54-55 23.41509236202641 26.807262109081975 26.351008179495906 23.42663734939571 56-57 23.18407047242693 27.110550230450954 26.36944277540062 23.335936521721496 58-59 23.260992473133985 26.80295447819387 26.451661025428606 23.484392023243537 60-61 23.39136072716423 26.82938312544706 26.485532669693114 23.293723477695597 62-63 23.280768060550656 26.910020120667266 26.561791441517794 23.247420377264287 64-65 23.181550444263184 26.72818144903536 26.480346600028966 23.609921506672492 66-67 23.13683465065601 27.048855448741264 26.244819941793608 23.56948995880912 68-69 23.07793666127736 26.80124004681167 26.579053409752866 23.54176988215811 70-71 22.961649705348982 26.778641048752277 26.68014042507571 23.579568820823027 72-73 23.211399120984474 26.816752634706503 26.372082534991144 23.59976570931788 74-75 23.035857558992838 27.0307480560718 26.302127721123693 23.63126666381167 76-77 23.197095084092098 26.74854055771152 26.529168637288976 23.525195720907412 78-79 23.09970370996419 26.859697152841928 26.42686185260826 23.61373728458563 80-81 23.0138023651068 26.91137869078193 26.533908892761875 23.540910051349393 82-83 23.186507226347075 26.935815562306203 26.434328998669 23.443348212677723 84-85 23.066565890901252 26.878357222672843 26.304729465283412 23.75034742114249 86-87 23.058758996093474 26.82371336842641 26.610483138914613 23.5070444965655 88-89 23.073151491263914 26.718989626132 26.521377957384924 23.686480925219165 90-91 22.991907889623228 26.899728296174757 26.671866427698927 23.43649738650309 92-93 23.143896420670487 26.817998752056273 26.569927392364857 23.468177434908387 94-95 23.036802641203643 26.629933480555433 26.632387339844904 23.700876538396017 96-97 23.26392538088424 26.620843830617762 26.460503617192956 23.654727171305044 98-99 22.849129197741085 26.621721728989456 26.50992188191304 24.019227191356414 100-101 22.808872617057276 26.582184055650345 26.771368679847285 23.83757464744509 102-103 22.956639005326178 26.463607280812713 26.71396321022808 23.865790503633033 104-105 22.916555880056922 26.487351891597164 26.730692464170723 23.86539976417519 106-107 22.71468756828778 26.545368895471142 26.85670091295225 23.883242623288826 108-109 22.795024362021337 26.56036052388272 26.559878388419673 24.084736725676265 110-111 22.675507210534914 26.600872615395787 26.71746524059318 24.00615493347612 112-113 22.69780316394307 26.54880345322435 26.923763038928183 23.82963034390439 114-115 22.66028168055406 26.731277545533437 26.687261413848063 23.921179360064436 116-117 22.408068111560475 26.68709124839052 26.842863544319595 24.061977095729414 118-119 22.567101912092525 26.450178390121327 26.77474063948179 24.207979058304357 120-121 22.411279250386915 26.581312594317772 26.937128666960298 24.070279488335014 122-123 22.545958853992364 26.571024225888973 26.827605374959585 24.055411545159075 124-125 22.518086826696802 26.50766343602262 26.66575606673024 24.308493670550334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 708.0 1 800.5 2 910.5 3 832.5 4 652.0 5 515.5 6 476.0 7 542.5 8 742.5 9 896.5 10 892.5 11 937.5 12 1048.0 13 1162.0 14 1291.5 15 1479.0 16 1613.5 17 1747.0 18 2012.5 19 2300.0 20 2706.0 21 3336.5 22 4153.5 23 5215.5 24 6639.0 25 9062.5 26 13051.0 27 17733.0 28 21576.5 29 26472.0 30 33408.0 31 39955.0 32 47063.5 33 55310.0 34 64377.5 35 72510.5 36 80810.5 37 89637.5 38 94540.0 39 97478.5 40 100336.5 41 104272.5 42 108618.0 43 113551.0 44 119613.5 45 124422.0 46 126151.5 47 126721.0 48 131515.5 49 134616.0 50 132934.5 51 130805.0 52 130360.5 53 130721.5 54 128639.0 55 121812.0 56 112698.0 57 104357.5 58 96786.0 59 87688.0 60 76427.5 61 63792.5 62 50753.5 63 39397.0 64 30171.5 65 23037.0 66 17211.0 67 13197.0 68 10129.0 69 7489.0 70 5446.0 71 3962.5 72 2954.5 73 2408.0 74 1893.5 75 1389.5 76 989.5 77 633.5 78 411.0 79 285.5 80 221.0 81 167.0 82 133.0 83 116.5 84 90.5 85 74.0 86 57.5 87 47.0 88 42.5 89 34.0 90 33.5 91 24.5 92 18.0 93 16.0 94 12.5 95 11.0 96 7.5 97 5.0 98 5.5 99 7.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012280273852943011 2 0.004395940986619323 3 0.007146949216955287 4 0.007090227397773101 5 0.014038650247590743 6 0.0010209927452793266 7 0.008338107419781167 8 0.0 9 0.0025524818631983163 10-11 0.0 12-13 0.003729459611228651 14-15 0.019767553984991405 16-17 0.031579872829681395 18-19 0.006281941474426967 20-21 0.00889114515680747 22-23 0.0023681359508562158 24-25 0.012450439310489567 26-27 0.02009370444528897 28-29 4.963159178441171E-4 30-31 0.0017867373042388217 32-33 0.009727791989744695 34-35 0.009997220630860074 36-37 0.012152649759783096 38-39 2.8360909591092404E-5 40-41 0.026347285010124847 42-43 0.010181566543202174 44-45 0.01759794440127284 46-47 0.004566106444165877 48-49 0.02599277364023619 50-51 0.0047788132660990705 52-53 0.0 54-55 0.018165162593094687 56-57 0.004977339633236717 58-59 2.9778955070647024E-4 60-61 0.004934798268850078 62-63 0.028290007317114677 64-65 0.018392049869823426 66-67 0.002226331402900754 68-69 0.022419299031758547 70-71 0.0032331436933845338 72-73 0.01262060476803612 74-75 2.8360909591092404E-5 76-77 0.006593911479928985 78-79 0.0012195191124169734 80-81 0.0038287227947974745 82-83 0.0017867373042388217 84-85 0.0 86-87 0.006055054197698228 88-89 0.017909914406774852 90-91 0.023199224045513587 92-93 0.005104963726396633 94-95 0.02610621727860056 96-97 0.018817463513689812 98-99 0.0010209927452793266 100-101 0.009174754252718393 102-103 0.0 104-105 0.0016307523014878133 106-107 4.254136438663861E-5 108-109 0.0 110-111 7.941054685505874E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.8360909591092404E-5 122-123 0.0 124-125 0.023582096324993335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3525980.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.40809296462503 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12426740331341 39.1340341553351 2 11.223444054949885 11.539517107202638 3 4.635642327178395 7.14928595119013 4 2.381758908350543 4.897667335192342 5 1.4047001619789594 3.6106478257219097 6 0.9197074812587515 2.8368244618086615 7 0.6445905593031666 2.319601997774378 8 0.4464747945044713 1.8361934193798175 9 0.33040782368535515 1.5287072504630554 >10 1.7183918816575523 15.702612424357962 >50 0.11568799620633667 4.093085951252719 >100 0.05352004397610331 4.727248026276852 >500 0.0012377760016116077 0.4125069808642647 >1k 1.6878763550761989E-4 0.21206711318019938 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4180454795546202E-4 2 0.0 0.0 0.0 5.672181918218481E-5 1.4180454795546202E-4 3 0.0 0.0 0.0 5.672181918218481E-5 1.4180454795546202E-4 4 0.0 0.0 0.0 1.7016545754655444E-4 1.4180454795546202E-4 5 0.0 0.0 0.0 1.7016545754655444E-4 1.4180454795546202E-4 6 0.0 0.0 0.0 1.7016545754655444E-4 1.4180454795546202E-4 7 0.0 0.0 0.0 1.9852636713764684E-4 1.4180454795546202E-4 8 0.0 0.0 0.0 1.9852636713764684E-4 1.4180454795546202E-4 9 0.0 0.0 0.0 1.9852636713764684E-4 1.4180454795546202E-4 10-11 0.0 2.8360909591092404E-5 0.0 1.9852636713764684E-4 1.4180454795546202E-4 12-13 0.0 2.8360909591092404E-5 0.0 2.5524818631983165E-4 5.672181918218481E-4 14-15 0.0 2.8360909591092404E-5 0.0 2.5524818631983165E-4 6.664813753906715E-4 16-17 0.0 2.8360909591092404E-5 0.0 3.4033091509310884E-4 7.090227397773102E-4 18-19 1.4180454795546202E-5 2.8360909591092404E-5 0.0 4.5377455345747847E-4 7.515641041639488E-4 20-21 2.8360909591092404E-5 2.8360909591092404E-5 0.0 5.104963726396633E-4 8.791881973238645E-4 22-23 2.8360909591092404E-5 2.8360909591092404E-5 0.0 6.523009205951253E-4 8.791881973238645E-4 24-25 2.8360909591092404E-5 2.8360909591092404E-5 0.0 7.799250137550412E-4 8.791881973238645E-4 26-27 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.0011060754740526038 9.500904713015956E-4 28-29 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.0016732936658744519 9.926318356882342E-4 30-31 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.0035592941536820967 9.926318356882342E-4 32-33 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.008394829238963352 0.0010209927452793266 34-35 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.01437898116268385 0.0010777145644615114 36-37 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.023908246785290896 0.0010918950192570576 38-39 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.04152037164135928 0.0011060754740526038 40-41 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.06332991111690935 0.0011060754740526038 42-43 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.08441624739788656 0.0011060754740526038 44-45 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.1099977878490519 0.00112025592884815 46-47 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.14204561568698632 0.0011486168384392424 48-49 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.17637649674700367 0.0012478800220080658 50-51 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.21664898836635488 0.0013613236603724356 52-53 2.8360909591092404E-5 2.8360909591092404E-5 0.0 0.2553474495034005 0.0014038650247590741 54-55 2.8360909591092404E-5 5.672181918218481E-5 0.0 0.29795971616401684 0.0014180454795546203 56-57 2.8360909591092404E-5 5.672181918218481E-5 0.0 0.34981763935132926 0.0014322259343501665 58-59 2.8360909591092404E-5 5.672181918218481E-5 0.0 0.4077164362815444 0.0014464063891457127 60-61 2.8360909591092404E-5 5.672181918218481E-5 0.0 0.47412350608908727 0.0014747672987368051 62-63 2.8360909591092404E-5 5.672181918218481E-5 0.0 0.5412821400007941 0.0015740304823056285 64-65 2.8360909591092404E-5 8.508272877327722E-5 0.0 0.611248503962019 0.001602391391896721 66-67 2.8360909591092404E-5 8.508272877327722E-5 0.0 0.6930271867679341 0.001616571846692267 68-69 2.8360909591092404E-5 8.508272877327722E-5 0.0 0.7875966397994316 0.001616571846692267 70-71 2.8360909591092404E-5 8.508272877327722E-5 0.0 0.8975377058293014 0.0016307523014878133 72-73 2.8360909591092404E-5 8.508272877327722E-5 0.0 1.0128389837718874 0.0017583763946477293 74-75 2.8360909591092404E-5 8.508272877327722E-5 0.0 1.131132337676334 0.0019143613973987375 76-77 2.8360909591092404E-5 8.508272877327722E-5 0.0 1.261124566787106 0.001956902761785376 78-79 2.8360909591092404E-5 8.508272877327722E-5 0.0 1.412557643548744 0.0019852636713764684 80-81 2.8360909591092404E-5 8.508272877327722E-5 0.0 1.5807661983335128 0.0019994441261720146 82-83 4.2541364386638604E-5 8.508272877327722E-5 0.0 1.7597093573985103 0.0022688727672873924 84-85 5.672181918218481E-5 8.508272877327722E-5 0.0 1.943431329729607 0.0022972336768784848 86-87 5.672181918218481E-5 8.508272877327722E-5 0.0 2.136640026318924 0.0024106773152428543 88-89 5.672181918218481E-5 8.508272877327722E-5 0.0 2.3577133165814894 0.002524120953607224 90-91 5.672181918218481E-5 8.508272877327722E-5 0.0 2.6084804791859284 0.002524120953607224 92-93 5.672181918218481E-5 8.508272877327722E-5 0.0 2.8768455861916404 0.0026659255015626863 94-95 5.672181918218481E-5 8.508272877327722E-5 0.0 3.149620814638767 0.0026801059563582325 96-97 5.672181918218481E-5 8.508272877327722E-5 0.0 3.430861774598835 0.002722647320744871 98-99 5.672181918218481E-5 8.508272877327722E-5 0.0 3.742037674632301 0.0027510082303359635 100-101 5.672181918218481E-5 8.508272877327722E-5 0.0 4.096988638619617 0.002779369139927056 102-103 5.672181918218481E-5 8.508272877327722E-5 0.0 4.481264783124124 0.002850271413904787 104-105 5.672181918218481E-5 8.508272877327722E-5 0.0 4.868362838132944 0.0028928127782914255 106-107 5.672181918218481E-5 8.508272877327722E-5 0.0 5.260282247772251 0.0028928127782914255 108-109 5.672181918218481E-5 8.508272877327722E-5 0.0 5.673727587791196 0.0028928127782914255 110-111 5.672181918218481E-5 8.508272877327722E-5 0.0 6.133387029988826 0.0028928127782914255 112-113 5.672181918218481E-5 8.508272877327722E-5 0.0 6.634850452923726 0.002921173687882518 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4115 0.0 54.80113 1 GTATCAA 7860 0.0 50.264996 1 ATCAACG 8765 0.0 44.394325 3 CAACGCA 8740 0.0 44.385162 5 TCAACGC 8770 0.0 44.369015 4 AACGCAG 8965 0.0 43.132347 6 TATCAAC 9825 0.0 39.54415 2 GTACATG 14285 0.0 36.112617 1 ACGCAGA 10890 0.0 35.070923 7 TACATGG 14990 0.0 33.618893 2 CGCAGAG 11310 0.0 32.877636 8 ACATGGG 14765 0.0 32.599937 3 GCAGAGT 12930 0.0 27.424393 9 CATGGGG 8770 0.0 26.594273 4 GAGTACA 10660 0.0 23.442947 1 AGTACAT 9935 0.0 23.355995 2 CATGGGA 7360 0.0 23.12011 4 CAGAGTA 12460 0.0 22.517849 10-11 AGAGTAC 11505 0.0 22.266388 10-11 GAGTACT 5380 0.0 21.681196 12-13 >>END_MODULE