FastQCFastQC Report
Thu 26 May 2016
SRR1364573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525980
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60140.17056251028082975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53800.15258169360007715No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.063.287291
GTATCAA95400.049.1196061
TCAACGC120450.038.713354
ATCAACG120900.038.6185153
CAACGCA121450.038.3096665
AACGCAG126000.036.973486
TATCAAC130200.035.7228282
GTACATG138450.032.3821451
ACGCAGA151000.030.458027
TACATGG143900.030.2111912
CGCAGAG152800.030.0213438
ACATGGG145050.028.6988963
GCAGAGT172050.025.8324179
CATGGGG85650.025.1702184
AGAGTAC158200.023.50467110-11
GAGTACT97350.023.46865712-13
GTACTTT103050.022.31487514-15
CAGAGTA168400.022.31063310-11
GAGTACA103200.021.8947621
AGTACTT110350.021.56654512-13
Produced by FastQC (version 0.11.5)