##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364573_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3525980 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.193397013029003 31.0 30.0 34.0 26.0 34.0 2 30.0631917934872 31.0 30.0 34.0 26.0 34.0 3 30.33778665789369 31.0 30.0 34.0 26.0 34.0 4 33.9228742080216 35.0 35.0 37.0 30.0 37.0 5 33.391816743146585 35.0 35.0 37.0 27.0 37.0 6 33.42479367438273 36.0 35.0 37.0 28.0 37.0 7 33.22011100460014 36.0 33.0 37.0 26.0 37.0 8 33.151097850810274 36.0 33.0 37.0 26.0 37.0 9 34.41243285554654 38.0 34.0 39.0 26.0 39.0 10-11 34.35576860901082 38.0 34.0 39.0 26.0 39.0 12-13 34.24923184476373 38.0 34.0 39.0 25.5 39.0 14-15 35.24030581568812 39.0 34.0 41.0 25.5 41.0 16-17 35.23640136926471 39.0 34.0 41.0 25.0 41.0 18-19 35.23033809040324 39.0 34.0 41.0 25.0 41.0 20-21 35.23098684621013 39.0 34.0 41.0 25.0 41.0 22-23 35.18776623803879 39.0 34.0 41.0 25.0 41.0 24-25 35.14100562113228 39.0 34.0 41.0 25.0 41.0 26-27 35.019280029949115 39.0 34.0 41.0 25.0 41.0 28-29 34.874382299389104 39.0 34.0 41.0 24.0 41.0 30-31 34.73565193222877 38.5 34.0 41.0 24.0 41.0 32-33 34.565675500144636 38.0 33.5 41.0 24.0 41.0 34-35 34.40277965274902 38.0 33.0 40.0 23.0 41.0 36-37 34.22442214646708 38.0 33.0 40.0 22.5 41.0 38-39 34.062975541551566 38.0 33.0 40.0 21.0 41.0 40-41 34.11332565698047 38.0 33.0 40.0 21.0 41.0 42-43 33.99617694938712 38.0 33.0 40.5 19.5 41.0 44-45 33.84864137062604 38.0 33.0 40.0 18.0 41.0 46-47 33.6444443814202 38.0 33.0 40.0 16.0 41.0 48-49 33.393752658835275 38.0 32.5 40.0 14.0 41.0 50-51 33.232384755443874 38.0 32.0 40.0 12.5 41.0 52-53 33.04363013970584 38.0 32.0 40.0 9.0 41.0 54-55 32.84110219002944 38.0 32.0 40.0 8.0 41.0 56-57 32.62057853419475 37.0 31.0 40.0 8.0 41.0 58-59 32.40872594285844 37.0 31.0 40.0 7.0 41.0 60-61 32.15806414103314 36.5 31.0 40.0 4.0 41.0 62-63 31.973558698574582 36.0 31.0 40.0 2.0 41.0 64-65 31.831614331334833 36.0 31.0 39.0 2.0 41.0 66-67 31.522784020329098 35.5 31.0 39.0 2.0 41.0 68-69 31.22723271260756 35.0 30.5 39.0 2.0 40.5 70-71 30.908744235645123 35.0 30.0 38.0 2.0 40.0 72-73 30.61702718109575 35.0 30.0 37.0 2.0 40.0 74-75 30.26567394029461 35.0 30.0 37.0 2.0 39.0 76-77 29.95650911236025 35.0 30.0 36.0 2.0 39.0 78-79 29.676139115933726 34.5 29.0 36.0 2.0 38.5 80-81 29.406442322418165 34.0 29.0 35.5 2.0 37.0 82-83 29.13432109654621 34.0 29.0 35.0 2.0 37.0 84-85 28.917371057124544 34.0 29.0 35.0 2.0 36.0 86-87 28.721223177669756 34.0 29.0 35.0 2.0 36.0 88-89 28.55616523633146 34.0 29.0 35.0 2.0 36.0 90-91 28.426586367477977 34.0 29.0 35.0 2.0 35.5 92-93 28.318487909744242 34.0 29.0 35.0 2.0 35.0 94-95 28.215681881349298 34.0 29.0 35.0 2.0 35.0 96-97 28.10613077782631 34.0 29.0 35.0 2.0 35.0 98-99 28.027555601563254 34.0 29.0 35.0 2.0 35.0 100-101 27.937914849205043 34.0 28.0 35.0 2.0 35.0 102-103 26.638426621818613 32.0 25.0 34.0 2.0 35.0 104-105 27.600654286184266 33.5 27.0 35.0 2.0 35.0 106-107 27.7701241924231 34.0 27.0 35.0 2.0 35.0 108-109 27.781669209694893 34.0 28.0 35.0 2.0 35.0 110-111 27.704184652210166 34.0 27.5 35.0 2.0 35.0 112-113 27.619064061622584 34.0 27.0 35.0 2.0 35.0 114-115 27.520723174833662 34.0 27.0 35.0 2.0 35.0 116-117 27.40144824984827 34.0 27.0 35.0 2.0 35.0 118-119 27.26517053414937 34.0 27.0 35.0 2.0 35.0 120-121 27.062928746050744 34.0 27.0 35.0 2.0 35.0 122-123 26.767307670491608 34.0 26.5 35.0 2.0 35.0 124-125 24.91256501738524 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.75081547340179 1101 2 0.8695400960524218 1101 3 0.6965610826498647 1101 4 0.6536859208935795 1101 5 0.800460503144123 1101 6 0.7431311441057531 1101 7 0.9034752912337396 1101 8 0.9789118480780061 1101 9 1.12049979843691 1101 10-11 1.1548727274514121 1101 12-13 0.9980587599189121 1101 14-15 1.1784110417405032 1101 16-17 1.0855780591621098 1101 18-19 1.126816067086608 1101 20-21 1.1674576441991462 1101 22-23 1.231551425893521 1101 24-25 1.2463044604959208 1101 26-27 1.2115973915069915 1101 28-29 1.359679578969235 1101 30-31 1.3282837489393131 1101 32-33 1.3041432649590945 1101 34-35 1.4562414738152256 1101 36-37 1.4352224075197384 1101 38-39 1.3827538239400354 1101 40-41 1.1265331032967083 1101 42-43 1.220701031782916 1101 44-45 1.1501339250729998 1101 46-47 1.2781215874302632 1101 48-49 1.333058200520675 1101 50-51 1.3590486219560063 1101 52-53 1.2793380834543555 1101 54-55 1.286657602440819 1101 56-57 1.2913321236964137 1101 58-59 1.3138805643851583 1101 60-61 1.2651992883630498 1101 62-63 1.2542650734249463 1101 64-65 1.2011250851745174 1101 66-67 1.4054114430560425 1101 68-69 1.3897111921517116 1101 70-71 1.331938408907284 1101 72-73 1.3334051236005315 1101 74-75 1.3638480075930453 1101 76-77 1.385460133862086 1101 78-79 1.3319637231440886 1101 80-81 1.1740928395698873 1101 82-83 1.3274648910057714 1101 84-85 1.4117016729176584 1101 86-87 1.3589139902927485 1101 88-89 1.345240494689314 1101 90-91 1.302026103534672 1101 92-93 1.3157674027559167 1101 94-95 1.3327815824091829 1101 96-97 1.2914636208102621 1101 98-99 1.3004098832302233 1101 100-101 1.3001521204183177 1101 102-103 1.2148755328960767 1101 104-105 1.351605216848835 1101 106-107 1.2718767531738635 1101 108-109 1.2291203393679453 1101 110-111 1.1774465672888539 1101 112-113 1.2628463191357149 1101 114-115 1.352512699061709 1101 116-117 1.2993080594606177 1101 118-119 1.2059250846755667 1101 120-121 1.1811996936553548 1101 122-123 1.1514354341377562 1101 124-125 1.43054543124493 1102 1 0.28535044921013863 1102 2 -0.49848377504799757 1102 3 0.10560432946364173 1102 4 0.15622242050202573 1102 5 0.4027670921948001 1102 6 0.29023253573711116 1102 7 0.3183680949021692 1102 8 0.43042520372033977 1102 9 0.7297697002030645 1102 10-11 0.7407908052241439 1102 12-13 0.7730234343907867 1102 14-15 0.9109945104539889 1102 16-17 0.8769622541849529 1102 18-19 0.9642502847071839 1102 20-21 0.8445670334239992 1102 22-23 0.9467241453345849 1102 24-25 0.9299633617335203 1102 26-27 1.0418484693922707 1102 28-29 0.6941875450378703 1102 30-31 0.8406063994676032 1102 32-33 0.9853279061029951 1102 34-35 1.0189971714192794 1102 36-37 0.7699259308802553 1102 38-39 0.1864219631420596 1102 40-41 -0.22205992275461028 1102 42-43 0.16252358449573023 1102 44-45 -0.11045758307073328 1102 46-47 0.11566673133391703 1102 48-49 -0.20908531658199792 1102 50-51 -0.3260076116296986 1102 52-53 -0.49652851155952504 1102 54-55 -0.46959765293122757 1102 56-57 -0.413063879009421 1102 58-59 -0.3180144321765681 1102 60-61 -0.36603305992132107 1102 62-63 -0.32098264720934466 1102 64-65 -0.3516478362024671 1102 66-67 -0.45240941968821957 1102 68-69 -0.22139135028458767 1102 70-71 -0.7319929894474129 1102 72-73 -0.5454351649433313 1102 74-75 -0.3421616926378839 1102 76-77 -0.7749891735631103 1102 78-79 -0.5882237849949234 1102 80-81 -0.6249492095935842 1102 82-83 -0.9886227934957184 1102 84-85 -0.7570689471440559 1102 86-87 -0.48162999337795753 1102 88-89 -0.5883443452085544 1102 90-91 -0.6313924942510454 1102 92-93 -0.4591215162829201 1102 94-95 -0.28101563639048166 1102 96-97 -0.5610043490413581 1102 98-99 -0.6781237081356188 1102 100-101 -0.5064508881146068 1102 102-103 -0.5661191561950787 1102 104-105 -0.4537314201137512 1102 106-107 -0.3331166678516304 1102 108-109 -0.2761227320850814 1102 110-111 -0.236522992358843 1102 112-113 -0.08919923223420412 1102 114-115 -0.09207067330942564 1102 116-117 -0.2044223708155073 1102 118-119 0.030394479922559725 1102 120-121 0.1398684206340981 1102 122-123 0.1174145419899233 1102 124-125 0.3112423355619498 1103 1 0.2706177606553162 1103 2 -0.18895256190652532 1103 3 0.1400360022421232 1103 4 0.24701234565910823 1103 5 0.22826139855752103 1103 6 0.34987990908674504 1103 7 0.4630447406242624 1103 8 0.5350989543726214 1103 9 0.6593256822750817 1103 10-11 0.6510141544982346 1103 12-13 0.5245143059855053 1103 14-15 0.7130079242774059 1103 16-17 0.5195547177106477 1103 18-19 0.6022705037317237 1103 20-21 0.6740331253656962 1103 22-23 0.7068131061832332 1103 24-25 0.6025278395844538 1103 26-27 0.6404890375407817 1103 28-29 0.7677741433665872 1103 30-31 0.6815629698463894 1103 32-33 0.7632175253119584 1103 34-35 0.7383706389980702 1103 36-37 0.8597079428465477 1103 38-39 0.8567901195187204 1103 40-41 0.7331330640281379 1103 42-43 0.8202995275379124 1103 44-45 0.7608659463410348 1103 46-47 0.8024492186635186 1103 48-49 0.6929733103685933 1103 50-51 0.6429610823006584 1103 52-53 0.7625650456592084 1103 54-55 0.6393740254229456 1103 56-57 0.6165529531408609 1103 58-59 0.6871292311947244 1103 60-61 0.752163972810834 1103 62-63 0.6974350005643117 1103 64-65 0.6439300340269227 1103 66-67 0.6599304579325356 1103 68-69 0.6672659628047022 1103 70-71 0.5485164892255696 1103 72-73 0.6197080159163271 1103 74-75 0.6742383416740552 1103 76-77 0.6711911213765909 1103 78-79 0.49476151448396166 1103 80-81 0.4390541715620131 1103 82-83 0.6457692061039744 1103 84-85 0.6413194639987445 1103 86-87 0.7118191963599116 1103 88-89 0.6924059425113427 1103 90-91 0.7167718170440125 1103 92-93 0.610155091553068 1103 94-95 0.5551649623528441 1103 96-97 0.678595738168152 1103 98-99 0.7259333211653498 1103 100-101 0.6746156234335032 1103 102-103 0.41168754249606465 1103 104-105 0.501716672356924 1103 106-107 0.6236963070649111 1103 108-109 0.6586872655559723 1103 110-111 0.5947833453421154 1103 112-113 0.5254732317203938 1103 114-115 0.7100271800499236 1103 116-117 0.7205845987969823 1103 118-119 0.593367609385794 1103 120-121 0.5745975528994727 1103 122-123 0.6986453755555786 1103 124-125 0.9523671595345604 1104 1 0.3816341942905872 1104 2 0.5139981080201679 1104 3 0.2294952089874407 1104 4 0.08383540053829819 1104 5 0.22727313959286732 1104 6 0.3200949968668567 1104 7 0.39985485243109053 1104 8 0.3740841894541518 1104 9 0.508495208196841 1104 10-11 0.4901577774478838 1104 12-13 0.4593566290748825 1104 14-15 0.5501874044454809 1104 16-17 0.5462479814795245 1104 18-19 0.4425597568589481 1104 20-21 0.3440101966358924 1104 22-23 0.5278716601938456 1104 24-25 0.5801628976698154 1104 26-27 0.4461883154211179 1104 28-29 0.5206016718091036 1104 30-31 0.4972361610555396 1104 32-33 0.5577246564820726 1104 34-35 0.7268887806140683 1104 36-37 0.7264711076198509 1104 38-39 0.8335411433255615 1104 40-41 0.6716450566330607 1104 42-43 0.6825689010595539 1104 44-45 0.7415242565226734 1104 46-47 0.7799215768604526 1104 48-49 0.7749003291092649 1104 50-51 0.6187114018809652 1104 52-53 0.6960980220273711 1104 54-55 0.30105557592167287 1104 56-57 0.5257753198917285 1104 58-59 0.664371790260283 1104 60-61 0.6449367533193282 1104 62-63 0.6915729667042214 1104 64-65 0.7613442437534914 1104 66-67 0.7779845644622903 1104 68-69 0.4984414980701608 1104 70-71 0.08878083780620827 1104 72-73 0.13321915312859645 1104 74-75 0.5904321585033578 1104 76-77 0.7249361776277752 1104 78-79 0.7397275638095486 1104 80-81 0.7362436247669883 1104 82-83 0.6635929946576447 1104 84-85 0.6504961093114474 1104 86-87 0.681640207551812 1104 88-89 0.6473979158432854 1104 90-91 0.6906047950388299 1104 92-93 0.7266818664647658 1104 94-95 0.6854689576815609 1104 96-97 0.757116856831022 1104 98-99 0.7345585186769128 1104 100-101 0.7713904005125727 1104 102-103 0.6268917471575897 1104 104-105 0.6780948150835364 1104 106-107 0.6932011314794444 1104 108-109 0.7005468162532722 1104 110-111 0.76441689232729 1104 112-113 0.7249602625655704 1104 114-115 0.6680218970888419 1104 116-117 0.6203920022343681 1104 118-119 0.654646816837861 1104 120-121 0.5538150873830148 1104 122-123 0.5957602553490737 1104 124-125 1.0420601619178775 1105 1 0.13367690111914143 1105 2 -0.13235576157827467 1105 3 0.1364620579863427 1105 4 0.16790292300657939 1105 5 -0.1535276739228948 1105 6 0.2662399549460659 1105 7 0.36483796363542353 1105 8 0.38614486582108754 1105 9 0.5326847731499598 1105 10-11 0.48613066204263333 1105 12-13 0.4906275673911651 1105 14-15 0.5122246927840024 1105 16-17 0.415105402408841 1105 18-19 0.5469429915944133 1105 20-21 0.516665065692834 1105 22-23 0.5648297106755606 1105 24-25 0.47519633677733 1105 26-27 0.4863371513907637 1105 28-29 0.5409828471884524 1105 30-31 0.636870164870885 1105 32-33 0.7150397658013503 1105 34-35 0.7285977802078207 1105 36-37 0.7265380140132365 1105 38-39 0.8664025947207605 1105 40-41 0.5748905252970502 1105 42-43 0.4713056409993186 1105 44-45 0.59366750163327 1105 46-47 0.4560154242282408 1105 48-49 0.5505621439914066 1105 50-51 0.3113258707476376 1105 52-53 0.4531785534764978 1105 54-55 0.37326570130913694 1105 56-57 0.45342023065192905 1105 58-59 0.09350943179720872 1105 60-61 0.08893422922666616 1105 62-63 0.3485666194970847 1105 64-65 0.1542769183274686 1105 66-67 0.37863975227819324 1105 68-69 0.5231642007942945 1105 70-71 0.5334045259409024 1105 72-73 0.5283274194942571 1105 74-75 0.4546919899459816 1105 76-77 0.5469356698677004 1105 78-79 0.45640055113155853 1105 80-81 0.35541444390261745 1105 82-83 0.38773159460201256 1105 84-85 0.36510200866155174 1105 86-87 0.47123100914951266 1105 88-89 0.46325389259793326 1105 90-91 0.3579426848338585 1105 92-93 0.3841524206070872 1105 94-95 0.4034118091062737 1105 96-97 0.40094976087983625 1105 98-99 0.2675510852975691 1105 100-101 0.2998189339347377 1105 102-103 0.04089356115589027 1105 104-105 0.27733165265096105 1105 106-107 0.31402749725817714 1105 108-109 0.36494606522471074 1105 110-111 0.2407908427783454 1105 112-113 0.2315811948578812 1105 114-115 0.29790304837914405 1105 116-117 0.008444341659195942 1105 118-119 0.1746294404193307 1105 120-121 0.2082724827181508 1105 122-123 0.17163000925381766 1105 124-125 0.6240007174258224 1106 1 -0.16953196410475257 1106 2 -0.5183098070093664 1106 3 0.005725639194405829 1106 4 -0.20515931183289382 1106 5 0.11600985903921668 1106 6 0.3460971612987507 1106 7 0.30132206527288474 1106 8 0.3571705071485596 1106 9 0.32732651808220936 1106 10-11 0.3503121245436702 1106 12-13 0.40878564068302836 1106 14-15 0.3466163774578064 1106 16-17 0.36864769885214344 1106 18-19 0.3762813452069693 1106 20-21 0.4242018479257723 1106 22-23 0.38565121418488246 1106 24-25 0.3681692014697191 1106 26-27 0.5595844684618001 1106 28-29 0.6248241044583338 1106 30-31 0.6333251298794451 1106 32-33 0.4681764074439343 1106 34-35 0.5018930616859123 1106 36-37 0.5620951569188435 1106 38-39 0.5389918708709018 1106 40-41 0.5187365263831936 1106 42-43 0.42744735972321024 1106 44-45 0.4277199490886332 1106 46-47 0.39688881958963407 1106 48-49 0.5770465011343688 1106 50-51 0.6237639374946724 1106 52-53 0.5200644932579408 1106 54-55 0.5752210246832092 1106 56-57 0.10038921179916116 1106 58-59 0.5423532659335493 1106 60-61 0.5717575255675627 1106 62-63 0.4164488922552394 1106 64-65 0.5668448393415062 1106 66-67 0.3356198787278011 1106 68-69 0.426873541750048 1106 70-71 0.4793348356724323 1106 72-73 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-1.6101312436914945 2216 50-51 -1.5329923904765792 2216 52-53 -1.4809565764524706 2216 54-55 -1.4060599615726943 2216 56-57 -1.3744640017632221 2216 58-59 -1.5106930087205157 2216 60-61 -1.3293221668741495 2216 62-63 -1.5261867930412834 2216 64-65 -1.3538401904548714 2216 66-67 -1.2013846588565507 2216 68-69 -1.0683096940737435 2216 70-71 -1.121214314445794 2216 72-73 -1.0626921797873088 2216 74-75 -1.021348424829828 2216 76-77 -1.1696514653352104 2216 78-79 -1.1392875628625312 2216 80-81 -1.0598868433416477 2216 82-83 -1.014406927168988 2216 84-85 -0.9677427058400028 2216 86-87 -1.164295501469418 2216 88-89 -1.0551587779576153 2216 90-91 -1.0751244114065557 2216 92-93 -1.1446254407972916 2216 94-95 -1.0326156398916702 2216 96-97 -1.1114686809455705 2216 98-99 -1.0848664275195787 2216 100-101 -1.2279207747079184 2216 102-103 -1.0629642402607438 2216 104-105 -0.918054902905407 2216 106-107 -1.0503171597156111 2216 108-109 -1.0728968905531318 2216 110-111 -1.034855246158962 2216 112-113 -0.9889399055446404 2216 114-115 -1.1226255082920353 2216 116-117 -1.103185960467691 2216 118-119 -1.1471964263230312 2216 120-121 -1.2474143199904084 2216 122-123 -1.1610240630727944 2216 124-125 -1.527633552068984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100202.0 3 33923.0 4 16600.0 5 13129.0 6 13741.0 7 16505.0 8 18549.0 9 20287.0 10 22281.0 11 24149.0 12 26306.0 13 28981.0 14 32472.0 15 29044.0 16 20869.0 17 18083.0 18 17455.0 19 16519.0 20 16615.0 21 17427.0 22 20035.0 23 24647.0 24 32559.0 25 42848.0 26 52759.0 27 61170.0 28 70971.0 29 84754.0 30 98933.0 31 117542.0 32 142869.0 33 178243.0 34 229055.0 35 317516.0 36 518609.0 37 772850.0 38 256928.0 39 555.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.170778598422906 19.438529970202406 13.701158744867184 24.689532686507505 2 15.61092678090255 20.48974199789619 38.748642575579716 25.150688645621543 3 19.34599742482941 24.520076687899532 29.038678608500334 27.095247278770724 4 13.187182872795955 16.70536613367484 36.209528443753385 33.89792254977582 5 13.626452787593804 38.192814479946 34.04330143676368 14.137431295696516 6 32.73424693276763 36.694535987158176 16.80352696271675 13.767690117357445 7 28.880241141132114 32.122163349973995 20.64229238362269 18.35530312527121 8 26.41152277147352 35.79432341893878 19.156525322704482 18.637628486883212 9 26.504029530509708 15.758151592029712 19.440332742931126 38.29748613452945 10-11 25.38789098056599 26.432117678072505 27.102275877608882 21.07771546375262 12-13 25.921913907622844 23.61188946051878 27.71274936329758 22.753447268560798 14-15 23.027951393911135 25.02857363262051 25.248654415881212 26.694820557587146 16-17 22.876180774033593 28.253905094511556 25.909331075042875 22.96058305641198 18-19 22.80502054788693 26.896914555391376 28.06906585817562 22.22899903854608 20-21 23.418018684124732 26.127314616876745 28.089163312591314 22.365503386407205 22-23 23.159873283455948 25.916369349797787 27.879851842608293 23.04390552413797 24-25 23.033965025326292 26.21502390824679 27.923031327460734 22.82797973896619 26-27 22.796754377506396 26.48821036988298 27.944571438295167 22.770463814315452 28-29 22.724760208509405 26.353056455226632 28.135185111656902 22.78699822460706 30-31 22.947152281067957 26.260032671767846 27.92227976335657 22.870535283807623 32-33 22.794074271550038 26.323660372435466 27.863076364585165 23.01918899142933 34-35 23.005221243455722 26.26727888416837 27.92846244164743 22.79903743072848 36-37 23.117686430439196 26.092717485635198 27.75163217034697 23.037963913578636 38-39 22.91757185236445 26.4537660451846 27.754581704944442 22.874080397506507 40-41 22.974733265645295 26.26048644632131 27.89879693021515 22.86598335781825 42-43 22.951789289786102 26.19826261067845 27.64228668341851 23.207661416116938 44-45 23.053534052944148 26.277105939341688 27.499829834542457 23.169530173171715 46-47 23.178889273336775 26.47306564416134 27.169411057351432 23.178634025150455 48-49 23.224195826408543 26.569294210403914 26.972628886153636 23.233881077033903 50-51 23.160106739149608 26.719401902280186 26.796245808610884 23.324245549959315 52-53 23.416085740701874 26.593925660383782 26.687927895223456 23.302060703690888 54-55 23.32796839460235 26.502490087862096 26.781703242786403 23.387838274749146 56-57 23.138340492698582 26.81294556944253 26.741881364897885 23.306832572961 58-59 23.23854863758935 26.51029961803267 26.829390383771095 23.421761360606883 60-61 23.3660287352736 26.540692233081298 26.895785001616574 23.19749403002853 62-63 23.192828660400796 26.64372741762574 26.981874542680334 23.181569379293133 64-65 23.09717609622317 26.41582316355499 26.9136010799865 23.57339966023534 66-67 23.094572289122457 26.780029949120525 26.670372492186566 23.455025269570445 68-69 23.007138867370045 26.54414184767665 26.944356880123664 23.50436240482964 70-71 22.929100562113227 26.485147391647146 27.026542975286304 23.559209070953322 72-73 23.220749975893227 26.57549106914957 26.670783725375642 23.532975229581563 74-75 23.038774002430554 26.75140990571322 26.54467100309279 23.66514508876344 76-77 23.152754695148584 26.535643991174084 26.79106234295146 23.52053897072587 78-79 23.077598284732186 26.595868382690767 26.72879596594422 23.597737366632824 80-81 22.970615374209103 26.654984796410297 26.786182494409704 23.588217334970892 82-83 23.11974259638455 26.674215395436164 26.65708540604314 23.548956602136144 84-85 23.01174425266167 26.646662771768415 26.554745063783685 23.78684791178623 86-87 22.976959597048197 26.58985586985746 26.83319247414903 23.599992058945315 88-89 22.95537653613444 26.48154610838851 26.753161890465126 23.80991546501192 90-91 22.892972009621722 26.644695466890834 26.906112188116804 23.55622033537064 92-93 23.02385328490515 26.526680995834123 26.80962934801059 23.639836371250134 94-95 22.896744011132224 26.375919088582332 26.845774344405577 23.881562555879864 96-97 23.113014554877935 26.360991208304043 26.677698181884708 23.848296054933314 98-99 22.710615126607177 26.341125075053313 26.727672685018295 24.220587113321216 100-101 22.62863486137263 26.35614657401491 27.003174516739175 24.01204404787329 102-103 22.848456315690957 26.204317664876147 26.8501664785393 24.097059540893596 104-105 22.863246530042712 26.21971763878411 26.848564087147402 24.068471744025775 106-107 22.64798223447431 26.33248533011149 26.91706868826097 24.102463747153234 108-109 22.658299629932618 26.283618495229483 26.681195962710504 24.376885912127396 110-111 22.585459659189446 26.330039352219163 26.79551324292717 24.288987745664215 112-113 22.578304471381006 26.294065763277157 26.943346247000832 24.184283518341 114-115 22.487790628421035 26.4282979483718 26.748265730378506 24.33564569282866 116-117 22.27020573003817 26.396363564172233 26.843359860237438 24.490070845552157 118-119 22.34937520916171 26.186776442294057 26.802676135429017 24.66117221311522 120-121 22.240384395858307 26.304936104426936 26.92882496723931 24.525854532475446 122-123 22.346833444395266 26.29270631532806 26.795370879972136 24.565089360304533 124-125 22.212017344034972 26.237494056616494 26.715306607623145 24.835181991725392 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 234.0 1 185.0 2 211.0 3 318.5 4 256.0 5 209.0 6 398.0 7 923.0 8 1540.0 9 1747.0 10 1435.0 11 1060.0 12 1012.5 13 1116.5 14 1270.5 15 1494.5 16 1687.5 17 1948.0 18 2261.5 19 2605.0 20 3147.5 21 3814.0 22 4707.5 23 5785.0 24 7087.5 25 9560.0 26 13730.5 27 18457.0 28 22189.5 29 26910.5 30 33913.0 31 40041.5 32 46921.0 33 55268.5 34 64033.5 35 71836.5 36 80234.0 37 89297.5 38 94099.5 39 96953.5 40 99647.5 41 103648.0 42 107370.5 43 112017.5 44 119023.5 45 124312.5 46 126047.5 47 127082.0 48 132369.5 49 135909.5 50 134128.0 51 131810.5 52 131343.0 53 131919.0 54 130352.0 55 123192.5 56 113610.5 57 105268.0 58 98125.0 59 88995.5 60 76982.0 61 63717.5 62 50275.0 63 38355.0 64 28553.5 65 21287.0 66 15615.5 67 11961.0 68 9223.5 69 6595.0 70 4672.5 71 3421.5 72 2574.0 73 2063.0 74 1562.5 75 1174.5 76 789.5 77 416.0 78 243.0 79 150.0 80 116.5 81 80.5 82 51.0 83 36.0 84 23.5 85 17.5 86 18.0 87 14.5 88 8.0 89 7.0 90 5.0 91 3.5 92 3.5 93 2.5 94 2.0 95 1.0 96 1.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10062450722919584 2 2.8360909591092404E-5 3 0.0 4 0.10031253722369385 5 0.0 6 0.0 7 0.0022121509481052076 8 0.006551370115542346 9 0.0038287227947974745 10-11 6.097595562084867E-4 12-13 0.0 14-15 5.672181918218481E-5 16-17 2.5524818631983165E-4 18-19 0.0019852636713764684 20-21 0.0012904213863947046 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.8360909591092404E-5 52-53 0.0 54-55 0.0 56-57 0.008366468329372259 58-59 6.806618301862178E-4 60-61 0.0 62-63 0.0 64-65 2.8360909591092405E-4 66-67 0.0 68-69 1.4180454795546202E-5 70-71 0.0 72-73 0.0 74-75 9.926318356882342E-4 76-77 0.0 78-79 0.0 80-81 9.926318356882342E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012336995672125196 90-91 1.4180454795546202E-5 92-93 0.002027805035763107 94-95 1.4180454795546202E-5 96-97 0.009174754252718393 98-99 0.004339219167437138 100-101 0.002524120953607224 102-103 0.0 104-105 0.0 106-107 0.0014180454795546201 108-109 1.4180454795546202E-5 110-111 9.926318356882342E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.933686521194108E-4 122-123 0.004807174175690163 124-125 0.08947866975989655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3525980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.4218814192906 #Duplication Level Percentage of deduplicated Percentage of total 1 71.17948250577338 31.619265313579348 2 12.965377457529852 11.518929199494693 3 5.555852936286041 7.40404320956148 4 2.922560321577813 5.193025121834137 5 1.8053751789409982 4.0099081058123796 6 1.1652245222590805 3.105687933278545 7 0.8300165062509403 2.5809626371713197 8 0.5656580069319578 2.0102074326242954 9 0.45092389909213726 1.8027799177135575 >10 2.3152789441524457 18.378340439330913 >50 0.16052823112588294 4.924028873213363 >100 0.08096281279376907 6.253755526609137 >500 0.002373745573396109 0.6759389247202735 >1k 2.5662114159185923E-4 0.19840993220329942 >5k 1.2831057079592962E-4 0.32471743285325966 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6014 0.17056251028082975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5380 0.15258169360007715 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8360909591092404E-5 2 0.0 0.0 0.0 0.0 2.8360909591092404E-5 3 0.0 0.0 0.0 0.0 2.8360909591092404E-5 4 0.0 0.0 0.0 0.0 2.8360909591092404E-5 5 0.0 2.8360909591092404E-5 0.0 0.0 5.672181918218481E-5 6 0.0 2.8360909591092404E-5 0.0 0.0 5.672181918218481E-5 7 0.0 2.8360909591092404E-5 0.0 2.8360909591092404E-5 5.672181918218481E-5 8 0.0 2.8360909591092404E-5 0.0 2.8360909591092404E-5 5.672181918218481E-5 9 0.0 2.8360909591092404E-5 0.0 5.672181918218481E-5 5.672181918218481E-5 10-11 0.0 2.8360909591092404E-5 0.0 5.672181918218481E-5 1.1344363836436962E-4 12-13 0.0 2.8360909591092404E-5 0.0 1.2762409315991583E-4 3.686918246842013E-4 14-15 0.0 2.8360909591092404E-5 0.0 1.7016545754655444E-4 4.821354630485709E-4 16-17 0.0 2.8360909591092404E-5 0.0 3.261504602975627E-4 5.672181918218481E-4 18-19 0.0 2.8360909591092404E-5 0.0 5.104963726396633E-4 5.672181918218481E-4 20-21 0.0 5.672181918218481E-5 0.0 5.530377370263019E-4 7.373836493684026E-4 22-23 0.0 5.672181918218481E-5 0.0 6.381204657995791E-4 8.508272877327721E-4 24-25 0.0 5.672181918218481E-5 0.0 7.799250137550412E-4 8.508272877327721E-4 26-27 0.0 5.672181918218481E-5 0.0 0.0011344363836436962 9.075491069149569E-4 28-29 0.0 5.672181918218481E-5 0.0 0.001744195939852183 9.075491069149569E-4 30-31 0.0 5.672181918218481E-5 0.0 0.003729459611228651 9.500904713015956E-4 32-33 0.0 8.508272877327722E-5 0.0 0.008338107419781167 0.0011344363836436962 34-35 0.0 8.508272877327722E-5 0.0 0.014761853442163596 0.0011627972932347886 36-37 0.0 8.508272877327722E-5 0.0 0.024915059075774678 0.0011627972932347886 38-39 0.0 8.508272877327722E-5 0.0 0.043307108945598104 0.0011769777480303348 40-41 0.0 8.508272877327722E-5 0.0 0.06569804706776555 0.001191158202825881 42-43 0.0 9.926318356882342E-5 0.0 0.08658585698160512 0.0012053386576214272 44-45 0.0 1.1344363836436962E-4 0.0 0.11230920198072593 0.0012904213863947044 46-47 0.0 1.1344363836436962E-4 0.0 0.1441017816323405 0.0014038650247590741 48-49 0.0 1.1344363836436962E-4 0.0 0.1784752040567445 0.0015456695727145361 50-51 0.0 1.1344363836436962E-4 0.0 0.218364823396616 0.001616571846692267 52-53 0.0 1.2762409315991583E-4 0.0 0.2571058258980482 0.001758376394647729 54-55 0.0 1.4180454795546202E-4 0.0 0.29926431800520703 0.0018009177590343677 56-57 0.0 1.4180454795546202E-4 0.0 0.35056920345549325 0.0018434591234210063 58-59 0.0 1.4180454795546202E-4 0.0 0.40883669221039254 0.0018434591234210063 60-61 0.0 1.4180454795546202E-4 0.0 0.4755982733878241 0.0018576395782165527 62-63 0.0 1.4180454795546202E-4 0.0 0.5422747718364823 0.0019001809426031913 64-65 0.0 1.4180454795546202E-4 0.0 0.6112910453264058 0.001956902761785376 66-67 0.0 1.4180454795546202E-4 0.0 0.6930839085871162 0.001956902761785376 68-69 0.0 1.4180454795546202E-4 0.0 0.7870010606980187 0.001956902761785376 70-71 0.0 1.4180454795546202E-4 0.0 0.8959353144374047 0.0019852636713764684 72-73 0.0 1.4180454795546202E-4 0.0 1.0105133891854179 0.0020703464001497456 74-75 0.0 1.4180454795546202E-4 0.0 1.1276864871610162 0.002155429128923023 76-77 0.0 1.4180454795546202E-4 0.0 1.257593633543015 0.002155429128923023 78-79 0.0 1.4180454795546202E-4 0.0 1.4088849057566974 0.002183790038514115 80-81 0.0 1.4180454795546202E-4 0.0 1.576200091889347 0.0022121509481052076 82-83 0.0 1.4180454795546202E-4 0.0 1.754065536389883 0.0023539554960606696 84-85 0.0 1.4180454795546202E-4 0.0 1.9378016891757754 0.0023539554960606696 86-87 1.4180454795546202E-5 1.4180454795546202E-4 0.0 2.1309536639459097 0.0024248577700384005 88-89 2.8360909591092404E-5 1.4180454795546202E-4 0.0 2.352438187397546 0.0024957600440161315 90-91 2.8360909591092404E-5 1.4180454795546202E-4 0.0 2.6044106886596063 0.0024957600440161315 92-93 2.8360909591092404E-5 1.4180454795546202E-4 0.0 2.873697525227029 0.0026375645919715935 94-95 2.8360909591092404E-5 1.4180454795546202E-4 0.0 3.146742182315271 0.00265174504676714 96-97 2.8360909591092404E-5 1.4180454795546202E-4 0.0 3.428805608653481 0.0030346173262468874 98-99 2.8360909591092404E-5 1.4180454795546202E-4 0.0 3.740690531426724 0.0033465873317489038 100-101 2.8360909591092404E-5 1.4180454795546202E-4 0.0 4.096775931797684 0.0033465873317489038 102-103 2.8360909591092404E-5 1.4180454795546202E-4 0.0 4.484171776357211 0.003374948241339996 104-105 2.8360909591092404E-5 1.4180454795546202E-4 0.0 4.873297636401794 0.0034033091509310886 106-107 2.8360909591092404E-5 1.4180454795546202E-4 0.0 5.268237482912552 0.0034033091509310886 108-109 2.8360909591092404E-5 1.4180454795546202E-4 0.0 5.6851853952659965 0.0034600309701132734 110-111 2.8360909591092404E-5 1.4180454795546202E-4 0.0 6.149382582998202 0.0034600309701132734 112-113 2.8360909591092404E-5 1.4180454795546202E-4 0.0 6.655029240097788 0.0034600309701132734 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5200 0.0 63.28729 1 GTATCAA 9540 0.0 49.119606 1 TCAACGC 12045 0.0 38.71335 4 ATCAACG 12090 0.0 38.618515 3 CAACGCA 12145 0.0 38.309666 5 AACGCAG 12600 0.0 36.97348 6 TATCAAC 13020 0.0 35.722828 2 GTACATG 13845 0.0 32.382145 1 ACGCAGA 15100 0.0 30.45802 7 TACATGG 14390 0.0 30.211191 2 CGCAGAG 15280 0.0 30.021343 8 ACATGGG 14505 0.0 28.698896 3 GCAGAGT 17205 0.0 25.832417 9 CATGGGG 8565 0.0 25.170218 4 AGAGTAC 15820 0.0 23.504671 10-11 GAGTACT 9735 0.0 23.468657 12-13 GTACTTT 10305 0.0 22.314875 14-15 CAGAGTA 16840 0.0 22.310633 10-11 GAGTACA 10320 0.0 21.894762 1 AGTACTT 11035 0.0 21.566545 12-13 >>END_MODULE