FastQCFastQC Report
Thu 26 May 2016
SRR1364572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359623
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44140.1870637809514486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.1327754476032824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87700.047.4238551
GGTATCA51850.041.5412061
ATCAACG104750.038.341493
CAACGCA105150.037.9692965
TCAACGC106200.037.6499184
AACGCAG107350.037.35196
TATCAAC110200.037.14722
GTACATG128700.036.5693471
TACATGG132950.034.1472322
ACATGGG138400.032.1576843
ACGCAGA125150.031.7066147
CGCAGAG123050.031.0919978
GCAGAGT132300.027.660059
CATGGGA84450.026.773384
CATGGGG56050.026.3266354
GAGTACT51600.025.25839612-13
ATGGGAG35650.024.033795
TAGGACG3002.237357E-1023.8001294
AGTACTT55450.022.96802112-13
AGAGTAC125050.022.31843210-11
Produced by FastQC (version 0.11.5)