FastQCFastQC Report
Thu 26 May 2016
SRR1364572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359623
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89320.3785350456407655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78150.331196975110007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49370.20922833859476705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69950.051.9163131
GTATCAA114700.046.6860581
ATCAACG146800.036.6814463
TCAACGC146750.036.5315864
CAACGCA146850.036.384675
TATCAAC148250.035.961062
AACGCAG150850.035.4198766
GTACATG124700.031.3697931
CGCAGAG172050.030.571288
ACGCAGA173250.030.3938737
TACATGG129150.029.610432
GCAGAGT183600.028.1283789
ACATGGG130700.027.983173
GAGTACT101600.027.64050312-13
GTACTTT107750.026.11809314-15
AGTACTT106200.025.54688512-13
CAGAGTA181850.024.01335110-11
AGAGTAC179200.023.25627510-11
CATGGGA83450.023.0558074
CATGGGG50650.021.9920794
Produced by FastQC (version 0.11.5)