##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2359623 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.108116423682937 31.0 30.0 34.0 26.0 34.0 2 29.97023507568794 31.0 30.0 34.0 26.0 34.0 3 30.278156298696867 31.0 30.0 34.0 26.0 34.0 4 33.84335251860149 35.0 35.0 37.0 30.0 37.0 5 33.26291530469062 35.0 33.0 37.0 27.0 37.0 6 33.293984250873976 36.0 35.0 37.0 27.0 37.0 7 33.087052889381056 36.0 33.0 37.0 26.0 37.0 8 33.01307242724791 36.0 33.0 37.0 26.0 37.0 9 34.23896020677879 38.0 34.0 39.0 25.0 39.0 10-11 34.18265799239963 38.0 34.0 39.0 25.0 39.0 12-13 34.05654716876383 38.0 34.0 39.0 25.0 39.0 14-15 35.026136802362075 39.0 34.0 41.0 25.0 41.0 16-17 35.03256855014551 39.0 34.0 41.0 25.0 41.0 18-19 35.071156917863576 39.0 34.0 41.0 25.0 41.0 20-21 35.09793068638507 39.0 34.0 41.0 25.0 41.0 22-23 35.06301663443694 39.0 34.0 41.0 25.0 41.0 24-25 35.0189064100494 39.0 34.0 41.0 25.0 41.0 26-27 34.89353701841354 39.0 34.0 41.0 24.0 41.0 28-29 34.743033950762474 39.0 34.0 41.0 24.0 41.0 30-31 34.56279117469189 38.5 33.5 41.0 24.0 41.0 32-33 34.341739760970285 38.0 33.0 40.5 23.0 41.0 34-35 34.14283235076112 38.0 33.0 40.0 22.0 41.0 36-37 33.93307574981257 38.0 33.0 40.0 19.5 41.0 38-39 33.754675852879885 38.0 33.0 40.0 18.0 41.0 40-41 33.763882620232124 38.0 33.0 40.0 18.0 41.0 42-43 33.614184130261485 38.0 33.0 40.0 15.5 41.0 44-45 33.42846738652742 38.0 33.0 40.0 15.0 41.0 46-47 33.14631320342275 38.0 32.0 40.0 9.5 41.0 48-49 32.8335142944445 38.0 32.0 40.0 7.5 41.0 50-51 32.646801205107764 38.0 31.0 40.0 4.0 41.0 52-53 32.4627451080109 38.0 31.0 40.0 2.0 41.0 54-55 32.26370865176344 37.0 31.0 40.0 2.0 41.0 56-57 32.05540291817803 37.0 31.0 40.0 2.0 41.0 58-59 31.84352224910505 37.0 31.0 40.0 2.0 41.0 60-61 31.606322069245806 36.0 30.0 40.0 2.0 41.0 62-63 31.426595689226627 36.0 30.0 40.0 2.0 41.0 64-65 31.29408511444413 36.0 30.0 39.0 2.0 41.0 66-67 30.99699358753496 35.0 30.0 39.0 2.0 41.0 68-69 30.71420604054122 35.0 30.0 39.0 2.0 41.0 70-71 30.40406518329411 35.0 29.5 38.0 2.0 40.0 72-73 30.112061333526583 35.0 29.0 37.0 2.0 40.0 74-75 29.761744143026238 35.0 29.0 37.0 2.0 39.0 76-77 29.451944865768812 35.0 29.0 36.0 2.0 39.0 78-79 29.159830405111325 34.0 29.0 36.0 2.0 39.0 80-81 28.88350766202906 34.0 29.0 35.5 2.0 37.0 82-83 28.6031073607945 34.0 29.0 35.0 2.0 37.0 84-85 28.37385463694836 34.0 28.5 35.0 2.0 36.5 86-87 28.155660671217394 34.0 28.0 35.0 2.0 36.0 88-89 27.974456936553 34.0 28.0 35.0 2.0 36.0 90-91 27.829194748483125 34.0 27.0 35.0 2.0 36.0 92-93 27.72186467923054 34.0 27.0 35.0 2.0 35.0 94-95 27.603214581312354 34.0 27.0 35.0 2.0 35.0 96-97 27.488414039022334 34.0 27.0 35.0 2.0 35.0 98-99 27.401200954559265 34.0 27.0 35.0 2.0 35.0 100-101 27.298405296100267 34.0 27.0 35.0 2.0 35.0 102-103 26.021899684822536 31.5 24.5 34.0 2.0 35.0 104-105 26.960140242742167 33.5 25.5 35.0 2.0 35.0 106-107 27.119531806564012 34.0 26.0 35.0 2.0 35.0 108-109 27.121184824863974 34.0 26.0 35.0 2.0 35.0 110-111 27.04682315776715 34.0 26.0 35.0 2.0 35.0 112-113 26.95838657277031 34.0 25.0 35.0 2.0 35.0 114-115 26.851948171381615 34.0 25.0 35.0 2.0 35.0 116-117 26.727486128080628 34.0 25.0 35.0 2.0 35.0 118-119 26.589046428179415 34.0 25.0 35.0 2.0 35.0 120-121 26.390990425165377 34.0 25.0 35.0 2.0 35.0 122-123 26.09924615076222 34.0 24.5 35.0 2.0 35.0 124-125 24.29073415541381 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6030099456981546 1101 2 0.8278298545783045 1101 3 0.653545890136094 1101 4 0.5391547273336386 1101 5 0.6843264991903979 1101 6 0.6444614041553862 1101 7 0.8295003438054707 1101 8 0.8018037862233669 1101 9 1.0101436254320006 1101 10-11 1.0157370434527522 1101 12-13 0.9440107436376906 1101 14-15 1.0669636555099942 1101 16-17 1.1515014249864421 1101 18-19 1.1571194679447103 1101 20-21 1.2001982418504724 1101 22-23 1.1478122551100256 1101 24-25 1.1593570591273945 1101 26-27 1.1743577859021386 1101 28-29 1.2193008760714505 1101 30-31 1.2828959053960745 1101 32-33 1.303024911927075 1101 34-35 1.4025257678141045 1101 36-37 1.337420709194788 1101 38-39 1.371908529234176 1101 40-41 1.1404858689449 1101 42-43 1.2620917425832658 1101 44-45 1.2197133033052623 1101 46-47 1.2777882451304237 1101 48-49 1.3628134405215704 1101 50-51 1.3558503361948908 1101 52-53 1.2971927032363624 1101 54-55 1.3554805252219992 1101 56-57 1.3759956689787245 1101 58-59 1.4726528958940044 1101 60-61 1.4135647666659708 1101 62-63 1.306114460691635 1101 64-65 1.322743715399838 1101 66-67 1.3505849279815934 1101 68-69 1.426373124374912 1101 70-71 1.4129583462579234 1101 72-73 1.3760630050563414 1101 74-75 1.4262414311032003 1101 76-77 1.5099411357152412 1101 78-79 1.3984895258152896 1101 80-81 1.1586731058795046 1101 82-83 1.3856623933862977 1101 84-85 1.3309736050926873 1101 86-87 1.3192542636032663 1101 88-89 1.2952949532410614 1101 90-91 1.3062740867141436 1101 92-93 1.2624429710119998 1101 94-95 1.2752044235110098 1101 96-97 1.3290706023529602 1101 98-99 1.309765255526333 1101 100-101 1.30517816247011 1101 102-103 1.1511891454890346 1101 104-105 1.2461790089169682 1101 106-107 1.2306242781914172 1101 108-109 1.2190171456830932 1101 110-111 1.0921435656374854 1101 112-113 1.2193202656279496 1101 114-115 1.21272177323565 1101 116-117 1.034292483930134 1101 118-119 1.087236564055619 1101 120-121 1.1320917368910948 1101 122-123 1.115608297180792 1101 124-125 1.3435980874297044 1102 1 0.32705423879817275 1102 2 -0.41037016425980255 1102 3 0.16330180582509612 1102 4 0.20880823697770268 1102 5 0.5146929396253483 1102 6 0.40155807242678065 1102 7 0.3740940032124911 1102 8 0.47510979557962685 1102 9 0.8092195116079708 1102 10-11 0.6871551539382494 1102 12-13 0.7525344639545892 1102 14-15 0.9229986525272906 1102 16-17 0.868222567360668 1102 18-19 1.0315333934923245 1102 20-21 0.8227873892691306 1102 22-23 0.9641042418500945 1102 24-25 0.9238846412563078 1102 26-27 1.0137020889444912 1102 28-29 0.6483644455265107 1102 30-31 0.8350017507038174 1102 32-33 0.9208420658553464 1102 34-35 1.0399649691306863 1102 36-37 0.7793703944414005 1102 38-39 0.27334925198956483 1102 40-41 -0.24817192244399422 1102 42-43 0.1990725499531436 1102 44-45 -0.18292605944343165 1102 46-47 -0.11607177464959051 1102 48-49 -0.29106773288320653 1102 50-51 -0.2788279050061462 1102 52-53 -0.5254216597739685 1102 54-55 -0.40421063447282535 1102 56-57 -0.43001728483651647 1102 58-59 -0.3433718906074468 1102 60-61 -0.45639783838264236 1102 62-63 -0.5278299119470624 1102 64-65 -0.45663015954652053 1102 66-67 -0.7128569773883093 1102 68-69 -0.4935613216277517 1102 70-71 -0.9004959870300588 1102 72-73 -0.6996328275356767 1102 74-75 -0.6111396206388022 1102 76-77 -0.9897937898742377 1102 78-79 -0.8813154016579965 1102 80-81 -0.868628740778302 1102 82-83 -1.1809115108637904 1102 84-85 -0.9756630339796395 1102 86-87 -0.7251071899660353 1102 88-89 -0.8511342115505158 1102 90-91 -0.7485938633858034 1102 92-93 -0.5885528322762994 1102 94-95 -0.4248137604954394 1102 96-97 -0.7702392557332978 1102 98-99 -0.8039268107524116 1102 100-101 -0.6320305990813146 1102 102-103 -0.6969528153486308 1102 104-105 -0.4788505237409808 1102 106-107 -0.38785286359673776 1102 108-109 -0.3961231001018817 1102 110-111 -0.39969832377235903 1102 112-113 -0.2862149001473995 1102 114-115 -0.3284975428887229 1102 116-117 -0.37710169300155627 1102 118-119 -0.17316069761615793 1102 120-121 0.08358001278564586 1102 122-123 0.010191425562496192 1102 124-125 0.24539660340546376 1103 1 0.3364959652141337 1103 2 -0.1179306890923506 1103 3 0.2651599869998087 1103 4 0.2885638765682188 1103 5 0.4435362009536803 1103 6 0.48305564422423686 1103 7 0.5480038516874117 1103 8 0.6370750011739972 1103 9 0.7172401169218361 1103 10-11 0.8294581860783481 1103 12-13 0.7621283216835479 1103 14-15 0.7664556133745748 1103 16-17 0.6189076498081434 1103 18-19 0.8455153835042495 1103 20-21 0.769522218737869 1103 22-23 0.7720861384696178 1103 24-25 0.8232528498261331 1103 26-27 0.832152987477734 1103 28-29 0.8419503124701819 1103 30-31 0.6817745925200214 1103 32-33 0.7693545460299376 1103 34-35 0.6832981850408189 1103 36-37 0.8517390634193944 1103 38-39 0.8925109158817719 1103 40-41 0.8934788374752642 1103 42-43 0.8634052939858634 1103 44-45 0.8790828111966178 1103 46-47 0.9853883418779148 1103 48-49 0.825792053985495 1103 50-51 0.7592477280960566 1103 52-53 0.8208898317518987 1103 54-55 0.9010648895077225 1103 56-57 0.8295152124436314 1103 58-59 0.8298768789066564 1103 60-61 0.8323211373156667 1103 62-63 0.8782952371530932 1103 64-65 0.8921657747707599 1103 66-67 0.9005402450887843 1103 68-69 0.8491603788349522 1103 70-71 0.7632874212356597 1103 72-73 0.8191578882761483 1103 74-75 0.9039451780268308 1103 76-77 0.9463790139296187 1103 78-79 0.8760806624255757 1103 80-81 0.9219395274315794 1103 82-83 0.9415100804772578 1103 84-85 0.8237184461425251 1103 86-87 0.7871312293756993 1103 88-89 0.7672688848173408 1103 90-91 0.7858435211025352 1103 92-93 0.6659916094009617 1103 94-95 0.650062207836342 1103 96-97 0.7448940004830327 1103 98-99 0.7771329227023891 1103 100-101 0.6993993451453981 1103 102-103 0.6846729661983417 1103 104-105 0.6421353641750258 1103 106-107 0.7940512518952616 1103 108-109 0.7717640441877478 1103 110-111 0.6663608689895675 1103 112-113 0.6306387015225248 1103 114-115 0.717380534145267 1103 116-117 0.7680946604346275 1103 118-119 0.7085175966780604 1103 120-121 0.7978280073066202 1103 122-123 0.7749793756483498 1103 124-125 1.0677164052169488 1104 1 0.3539255729908888 1104 2 0.595937280148835 1104 3 0.22196261794240257 1104 4 0.16094342872762724 1104 5 0.3514052341790048 1104 6 0.5427002735224917 1104 7 0.525514322258573 1104 8 0.45105227060379605 1104 9 0.42073095214938405 1104 10-11 0.5731935442057292 1104 12-13 0.6954107127460816 1104 14-15 0.5732490553632559 1104 16-17 0.6035381087722058 1104 18-19 0.5255739993576185 1104 20-21 0.46883198321047104 1104 22-23 0.5778446140705285 1104 24-25 0.6721140966220815 1104 26-27 0.6255059883966325 1104 28-29 0.8405226392100431 1104 30-31 0.7729793127405529 1104 32-33 0.7346642137767105 1104 34-35 0.9035092408032526 1104 36-37 1.047218484998929 1104 38-39 1.153101910706937 1104 40-41 1.0356284723352545 1104 42-43 0.9443503053511506 1104 44-45 0.9371686070083882 1104 46-47 0.9750493178415454 1104 48-49 0.9790895342776054 1104 50-51 0.8938239238691352 1104 52-53 0.9548496905321855 1104 54-55 0.5394435711346546 1104 56-57 0.7692468949894575 1104 58-59 0.8772170152900749 1104 60-61 0.9289372808568075 1104 62-63 0.9738899559227399 1104 64-65 0.995159439228857 1104 66-67 0.9946910406587435 1104 68-69 0.7244800093422796 1104 70-71 0.40224200845818103 1104 72-73 0.3168881199345108 1104 74-75 0.7811484473599073 1104 76-77 0.9203547357461339 1104 78-79 0.9110035235756477 1104 80-81 0.8671903858733252 1104 82-83 0.9658735761487769 1104 84-85 0.9391440881602229 1104 86-87 0.8855000449679018 1104 88-89 0.9840392093325008 1104 90-91 0.9563924401913795 1104 92-93 0.8538519373015276 1104 94-95 0.8824554950636987 1104 96-97 0.9619670768480724 1104 98-99 0.9938722949517462 1104 100-101 0.8944738723978993 1104 102-103 0.7433625246449225 1104 104-105 0.9139041894398119 1104 106-107 0.827778581778702 1104 108-109 0.8185338850737551 1104 110-111 0.8290504274361474 1104 112-113 0.8353966451313646 1104 114-115 0.8571060262379255 1104 116-117 0.7461052035501687 1104 118-119 0.6578988415394988 1104 120-121 0.6494300772393977 1104 122-123 0.727317916055565 1104 124-125 1.0473405286391113 1105 1 0.24958826405819678 1105 2 -0.007029572893930691 1105 3 0.25110385031459614 1105 4 0.1678592923232216 1105 5 -0.05460419276219852 1105 6 0.4342979301452061 1105 7 0.5163320998667302 1105 8 0.603007082351219 1105 9 0.7210473462489588 1105 10-11 0.6449580615043615 1105 12-13 0.6508355930010339 1105 14-15 0.7362683099860803 1105 16-17 0.7291662979980131 1105 18-19 0.7325791708172673 1105 20-21 0.7016260598854771 1105 22-23 0.726750172912574 1105 24-25 0.6991381982956497 1105 26-27 0.6287741219004417 1105 28-29 0.7014615167552236 1105 30-31 0.803644351001715 1105 32-33 0.8396074040520816 1105 34-35 0.8692601329277565 1105 36-37 0.8886742795145111 1105 38-39 0.8274467001273891 1105 40-41 0.7079956650532324 1105 42-43 0.7101833683437278 1105 44-45 0.7580603498475327 1105 46-47 0.7195242524826995 1105 48-49 0.74366221141441 1105 50-51 0.5452055623249521 1105 52-53 0.6515420173073636 1105 54-55 0.5448691383731727 1105 56-57 0.533637330651537 1105 58-59 0.1588673406531278 1105 60-61 0.26713455963855637 1105 62-63 0.5255536392996483 1105 64-65 0.2313109954374717 1105 66-67 0.4997149133377832 1105 68-69 0.5918630599309864 1105 70-71 0.592526992142318 1105 72-73 0.6174023653021443 1105 74-75 0.5587585460183817 1105 76-77 0.5954679162892305 1105 78-79 0.5203283713897058 1105 80-81 0.5170658128526959 1105 82-83 0.5441897794195825 1105 84-85 0.5617948142636919 1105 86-87 0.6810726648560781 1105 88-89 0.5771094534024996 1105 90-91 0.4101782921464192 1105 92-93 0.49337673550010663 1105 94-95 0.47146673487820934 1105 96-97 0.5533477229942854 1105 98-99 0.4558653106187869 1105 100-101 0.5469072445330845 1105 102-103 0.1988596145061905 1105 104-105 0.438899972124009 1105 106-107 0.4314535622187563 1105 108-109 0.5256701177321901 1105 110-111 0.38987236511147216 1105 112-113 0.337858908742227 1105 114-115 0.36611112136607105 1105 116-117 0.18425691242179454 1105 118-119 0.30881894153377587 1105 120-121 0.3341949952784411 1105 122-123 0.41003348577485355 1105 124-125 0.9797655424741976 1106 1 0.01819015156197068 1106 2 -0.3336120608365256 1106 3 0.045563415568977916 1106 4 -0.043582421182399855 1106 5 0.28161563250593247 1106 6 0.41016314011458377 1106 7 0.5146728262223874 1106 8 0.4402092182230817 1106 9 0.5475029963106621 1106 10-11 0.39964712136637814 1106 12-13 0.49008801443374495 1106 14-15 0.5387991745208822 1106 16-17 0.46081781306674685 1106 18-19 0.5024770682745014 1106 20-21 0.5428651528033939 1106 22-23 0.5011059340452277 1106 24-25 0.6377688799497463 1106 26-27 0.5886264591908557 1106 28-29 0.6736076058945386 1106 30-31 0.6118529083827937 1106 32-33 0.5751610767697599 1106 34-35 0.6430757988500417 1106 36-37 0.7026922460027691 1106 38-39 0.541063585722739 1106 40-41 0.6343540207035261 1106 42-43 0.5902576055797368 1106 44-45 0.5682167077816942 1106 46-47 0.4592932748475249 1106 48-49 0.6465533735850357 1106 50-51 0.6380593209801688 1106 52-53 0.6099256109640692 1106 54-55 0.705801822192484 1106 56-57 0.15927286648889805 1106 58-59 0.7154332499090081 1106 60-61 0.7445699835463628 1106 62-63 0.665081240393345 1106 64-65 0.715357873157167 1106 66-67 0.5697648211168911 1106 68-69 0.6721473406977587 1106 70-71 0.7120687678570405 1106 72-73 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-1.5787961273761937 2216 52-53 -1.5919066870958645 2216 54-55 -1.682852150656693 2216 56-57 -1.5595023220957707 2216 58-59 -1.7162383175888962 2216 60-61 -1.478666767006679 2216 62-63 -1.6863226863175562 2216 64-65 -1.4942653880122663 2216 66-67 -1.4799862086575573 2216 68-69 -1.4141515160258855 2216 70-71 -1.5426633602235036 2216 72-73 -1.4094237864090395 2216 74-75 -1.2752628956300924 2216 76-77 -1.4196289071584616 2216 78-79 -1.4877205194566265 2216 80-81 -1.4251589450904127 2216 82-83 -1.2009999928210533 2216 84-85 -1.1831093605469967 2216 86-87 -1.3415837851460637 2216 88-89 -1.4070354759918366 2216 90-91 -1.3789233570013408 2216 92-93 -1.41898348236667 2216 94-95 -1.259353654902224 2216 96-97 -1.354153461831796 2216 98-99 -1.3696527626345478 2216 100-101 -1.4271342647394327 2216 102-103 -1.3425981182510398 2216 104-105 -1.3304274731001975 2216 106-107 -1.3270187230965043 2216 108-109 -1.3665164544049944 2216 110-111 -1.3245079137335232 2216 112-113 -1.2605340960279534 2216 114-115 -1.3102813763830063 2216 116-117 -1.3913311150681054 2216 118-119 -1.4001262634961726 2216 120-121 -1.4627889490993198 2216 122-123 -1.5181565001013801 2216 124-125 -1.8423187157942138 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70507.0 3 23982.0 4 11700.0 5 9162.0 6 10375.0 7 12347.0 8 13961.0 9 15128.0 10 16660.0 11 18657.0 12 20562.0 13 24000.0 14 28261.0 15 24739.0 16 16596.0 17 14398.0 18 13410.0 19 12399.0 20 12271.0 21 12513.0 22 14040.0 23 17460.0 24 22465.0 25 29212.0 26 36996.0 27 41902.0 28 47286.0 29 55736.0 30 65143.0 31 75688.0 32 92611.0 33 114712.0 34 149259.0 35 205320.0 36 328154.0 37 496589.0 38 184678.0 39 744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.815952257932054 19.74081738099056 14.0197358541471 23.423494506930286 2 15.394499627482707 20.757816294304764 39.36338108391937 24.484302994293152 3 18.97765024328039 25.76725180251252 29.59519380850246 25.659904145704633 4 12.559699713305623 17.242770490927 36.753090724898115 33.44443907086925 5 12.982624766752995 38.89371310586479 33.72445513541782 14.399206991964395 6 31.374884886272085 37.53137683435023 17.082940791812927 14.01079748756475 7 27.8566476124605 33.38512212322172 20.677670301205637 18.08055996311214 8 26.235803003884755 35.98634952307248 19.08773889818002 18.690108574862744 9 26.74829850093472 16.081460049153712 19.622239447823876 37.5480020020877 10-11 25.078386778173627 27.20757022283522 26.75232617545204 20.96171682353911 12-13 26.001929121728345 24.42671986160501 27.74758933948347 21.823761677183175 14-15 22.827766481241486 25.465371148429703 26.145670789643425 25.56119158068538 16-17 22.077547265045755 29.172802897416915 26.575603942839926 22.174045894697407 18-19 21.78538318830384 26.826497540343556 29.826504956925554 21.561614314427054 20-21 22.557439966367095 26.049629724435647 29.90427590509215 21.488654404105112 22-23 22.201088902761164 26.01095598746071 29.804845943610488 21.983109166167647 24-25 22.115206539349717 26.227897422596747 29.86468601128231 21.792210026771226 26-27 21.892310763202428 26.522118151925117 29.732164841586982 21.853406243285477 28-29 21.60268822604289 26.441490865278055 30.142590574850303 21.813230333828752 30-31 21.734446562014355 26.21183553474432 30.110678697402083 21.943039205839238 32-33 21.72976361054287 26.339313525931896 29.84917505889712 22.08174780462811 34-35 22.215731072294176 26.359676948393872 29.558683738885406 21.865908240426542 36-37 22.334690753565294 25.995042428387926 29.528657755921177 22.141609062125603 38-39 22.031421968678895 26.319352710157514 29.62935180747094 22.01987351369265 40-41 22.062761720834217 26.131843942867146 29.80738872269002 21.998005613608616 42-43 22.23374242410758 26.14911365078235 29.320043922270635 22.297100002839436 44-45 22.19231631493675 26.50376776290111 29.014783293771927 22.289132628390213 46-47 22.315747049422725 26.764741655764503 28.37940637127202 22.540104923540753 48-49 22.58968911559177 26.833184792655434 28.057850766838598 22.519275324914194 50-51 22.542996293474125 27.0206202518878 27.82680870071562 22.609574753922452 52-53 22.758677975252827 26.897114496680192 27.782997538166054 22.56120998990093 54-55 22.355520352191853 26.876539175961582 28.02187044286312 22.746070028983443 56-57 22.430702115571385 27.283138092258163 27.740620788846137 22.545539003324315 58-59 22.557329389178722 26.81061700874297 27.88053958069342 22.75151402138489 60-61 22.624122582293865 26.881073798653425 28.161574963458147 22.33322865559456 62-63 22.42044597802276 26.9171176921059 28.114512360661003 22.547923969210334 64-65 22.290453547605125 26.68410591535925 28.077275154474023 22.9481653825616 66-67 22.155318879329453 27.194937496371242 27.91467111483487 22.735072509464434 68-69 22.21922739352854 26.978462237399786 28.08069339890313 22.72161697016854 70-71 22.174898278241905 26.962845335886282 27.977265859842866 22.884990526028943 72-73 22.397433827352927 26.96543049461715 27.782488982350145 22.854646695679776 74-75 22.26369603507053 27.147154503243787 27.662774179221117 22.926375282464566 76-77 22.477722076789387 27.038747291410537 27.741868086554504 22.741662545245575 78-79 22.52599249964931 27.07065916885875 27.673446139489233 22.729902192002704 80-81 22.105722865781043 27.48156272487712 27.644152326092907 22.768562083248927 82-83 22.423476123092545 27.35667943565561 27.453008383118828 22.766836058133016 84-85 22.355011796375944 27.05040169552509 27.410353264059555 23.184233244039408 86-87 22.45282403163556 26.82638285861767 27.68376558458703 23.03702752515974 88-89 22.383878623495505 26.924775385658585 27.57507628411595 23.116269706729955 90-91 22.363614865595054 27.192543046071343 27.7359985048459 22.7078435834877 92-93 22.360342816336434 27.003281314729684 27.64610962367124 22.990266245262646 94-95 22.26190370241348 26.799853196887806 27.667110381616045 23.27113271908267 96-97 22.41993576381972 26.797879816124148 27.52937376929395 23.25281065076218 98-99 22.237255796537745 26.78646012018901 27.395945003535655 23.580339079737584 100-101 21.982451452458363 26.857389391323032 27.64816956998705 23.51198958623155 102-103 22.38014292961206 26.66709893911019 27.494031885602066 23.458726245675688 104-105 22.14728793540324 26.62575758924201 27.735744226937946 23.491210248416802 106-107 22.19484689263286 26.768276299316913 27.647794994545656 23.38908181350457 108-109 22.22999653546277 26.803736614649164 27.22293502456431 23.743331825323754 110-111 21.921260136174585 26.828240842373642 27.660367059413854 23.590131962037923 112-113 22.196829747802933 26.6587713376247 27.53700485204628 23.60739406252609 114-115 21.834907525481825 26.9616375158235 27.345003841715393 23.85845111697928 116-117 21.840692347887778 26.84721245724423 27.482970796606065 23.829124398261925 118-119 21.815518835000336 26.96924466323646 27.196547923121617 24.01868857864159 120-121 21.67920513797969 27.05400417739498 27.3974303744547 23.869360310170624 122-123 21.84611433352066 26.98536311229666 27.20133942610313 23.967183128079547 124-125 21.7275930703705 26.89412665438701 27.218402944572933 24.15987733066956 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 436.0 1 331.5 2 449.5 3 720.0 4 546.0 5 397.5 6 842.5 7 1998.0 8 3314.5 9 3841.0 10 2988.0 11 1875.5 12 1614.5 13 1703.0 14 1871.0 15 2176.5 16 2504.0 17 2804.5 18 3200.5 19 3700.5 20 4247.0 21 4918.5 22 5878.0 23 6850.5 24 8109.0 25 10057.0 26 13088.0 27 16850.5 28 20229.0 29 23766.5 30 27620.5 31 32454.5 32 38226.5 33 43961.5 34 49203.5 35 52916.5 36 57536.0 37 63535.5 38 67611.5 39 68671.5 40 69017.0 41 72849.5 42 76618.0 43 77965.5 44 79355.0 45 81198.0 46 81026.5 47 81255.5 48 88054.0 49 90261.0 50 86884.0 51 83960.5 52 80483.5 53 79470.0 54 76980.0 55 71495.0 56 65612.5 57 60182.0 58 55611.0 59 49806.5 60 42351.0 61 34600.5 62 27967.5 63 21971.5 64 17137.0 65 13505.0 66 9891.0 67 8125.0 68 6297.0 69 4062.5 70 2935.0 71 2304.0 72 1921.5 73 1592.0 74 1270.5 75 1069.5 76 735.5 77 377.5 78 221.0 79 127.5 80 75.5 81 56.5 82 37.0 83 24.0 84 17.5 85 11.0 86 8.0 87 7.5 88 4.5 89 1.5 90 1.0 91 0.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09700702188442815 2 4.2379651325656687E-5 3 0.0 4 0.10209258004350696 5 0.0 6 0.0 7 0.0016528064017006109 8 0.006145049442220218 9 0.003644650014006475 10-11 4.0260668759373847E-4 12-13 0.0 14-15 4.2379651325656687E-5 16-17 1.6951860530262675E-4 18-19 0.0019282741353173793 20-21 0.001229009888444044 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.2379651325656687E-5 52-53 0.0 54-55 0.0 56-57 0.008030943926211941 58-59 5.509354672335369E-4 60-61 0.0 62-63 0.0 64-65 1.2713895397697004E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0014832877963979838 76-77 0.0 78-79 0.0 80-81 4.2379651325656687E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.747319804901037E-4 90-91 0.0 92-93 0.002140172391945662 94-95 0.0 96-97 0.00868782852175962 98-99 0.0037929787936462726 100-101 0.0023096909972482892 102-103 0.0 104-105 0.0 106-107 0.0015680470990492973 108-109 2.1189825662828343E-5 110-111 2.118982566282834E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 7.840235495246487E-4 122-123 0.0044498633891939515 124-125 0.08753516981314387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2359623.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.012784141824575 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03114891833431 34.80410423868921 2 12.669358536939445 11.91243636225033 3 5.07219159419219 7.153735456312034 4 2.487238199923123 4.677279704091443 5 1.4493272149453638 3.406845375354908 6 0.9338611504921851 2.634204761191503 7 0.6110358646829928 2.0108548046478267 8 0.43294422808774363 1.6283130832430364 9 0.3201204452758955 1.3544778053826396 >10 1.751206228846495 15.158181667851498 >50 0.14509846412530758 4.718784693683677 >100 0.0904949772079208 7.576404006128791 >500 0.00479744512419343 1.4456768707254364 >1k 9.956961578514172E-4 0.8060333689040796 >5k 1.8103566506389403E-4 0.7126678015435628 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8932 0.3785350456407655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7815 0.331196975110007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4937 0.20922833859476705 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.2379651325656687E-5 0.0 0.0 4.2379651325656687E-5 0.0 3 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 4 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 5 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 6 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 7 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 8 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 9 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 10-11 4.2379651325656687E-5 0.0 0.0 8.475930265131337E-5 0.0 12-13 4.2379651325656687E-5 0.0 0.0 1.2713895397697004E-4 0.0 14-15 4.2379651325656687E-5 0.0 0.0 2.3308808229111175E-4 0.0 16-17 4.2379651325656687E-5 0.0 0.0 4.0260668759373847E-4 2.1189825662828343E-5 18-19 6.356947698848503E-5 0.0 0.0 5.297456415707085E-4 4.2379651325656687E-5 20-21 8.475930265131337E-5 0.0 0.0 7.416438981989919E-4 4.2379651325656687E-5 22-23 8.475930265131337E-5 0.0 0.0 0.0010806811088042456 4.2379651325656687E-5 24-25 1.2713895397697004E-4 0.0 0.0 0.001737565704351924 4.2379651325656687E-5 26-27 1.2713895397697004E-4 0.0 0.0 0.0022249316945969757 4.2379651325656687E-5 28-29 1.483287796397984E-4 0.0 0.0 0.0033691822803897063 4.2379651325656687E-5 30-31 1.6951860530262675E-4 0.0 0.0 0.005806012231614966 4.2379651325656687E-5 32-33 1.6951860530262675E-4 0.0 0.0 0.010933950042019424 4.2379651325656687E-5 34-35 1.6951860530262675E-4 0.0 0.0 0.017545175648821867 4.2379651325656687E-5 36-37 2.1189825662828343E-4 0.0 0.0 0.029008871332412 4.2379651325656687E-5 38-39 2.1189825662828343E-4 0.0 0.0 0.048990876932459126 4.2379651325656687E-5 40-41 2.1189825662828343E-4 0.0 0.0 0.07458818633315575 4.2379651325656687E-5 42-43 2.1189825662828343E-4 0.0 0.0 0.09829960124986067 4.2379651325656687E-5 44-45 2.1189825662828343E-4 0.0 0.0 0.12491402228237308 4.2379651325656687E-5 46-47 3.178473849424251E-4 0.0 0.0 0.1575887334544544 4.2379651325656687E-5 48-49 3.8141686193091014E-4 0.0 0.0 0.19511591470332337 4.2379651325656687E-5 50-51 3.8141686193091014E-4 0.0 0.0 0.2417971006385342 4.2379651325656687E-5 52-53 3.8141686193091014E-4 0.0 0.0 0.2859355074942056 4.2379651325656687E-5 54-55 3.8141686193091014E-4 0.0 0.0 0.332425984998451 4.2379651325656687E-5 56-57 4.2379651325656685E-4 0.0 0.0 0.38472247473431137 4.2379651325656687E-5 58-59 4.2379651325656685E-4 0.0 0.0 0.44820719242014506 4.2379651325656687E-5 60-61 4.2379651325656685E-4 0.0 0.0 0.5217146976444966 4.2379651325656687E-5 62-63 4.2379651325656685E-4 0.0 0.0 0.59486197583258 4.2379651325656687E-5 64-65 4.2379651325656685E-4 0.0 0.0 0.668305911579943 4.2379651325656687E-5 66-67 4.2379651325656685E-4 0.0 0.0 0.7514759773065443 4.2379651325656687E-5 68-69 4.2379651325656685E-4 0.0 0.0 0.8558994381729623 4.2379651325656687E-5 70-71 5.085558159078803E-4 0.0 0.0 0.9771052409643405 4.2379651325656687E-5 72-73 5.509354672335369E-4 0.0 0.0 1.1036508798227513 4.2379651325656687E-5 74-75 5.509354672335369E-4 0.0 0.0 1.2259797433742594 4.2379651325656687E-5 76-77 5.509354672335369E-4 0.0 0.0 1.3573990421351207 4.2379651325656687E-5 78-79 5.509354672335369E-4 0.0 0.0 1.5152632433231918 4.2379651325656687E-5 80-81 5.509354672335369E-4 0.0 0.0 1.6972838457668873 4.2379651325656687E-5 82-83 5.509354672335369E-4 0.0 0.0 1.8873565819624576 4.2379651325656687E-5 84-85 5.721252928963653E-4 0.0 0.0 2.0803535140994978 4.2379651325656687E-5 86-87 6.145049442220218E-4 0.0 0.0 2.2813813901627507 4.2379651325656687E-5 88-89 6.356947698848502E-4 0.0 0.0 2.5122233509336027 4.2379651325656687E-5 90-91 6.356947698848502E-4 0.0 0.0 2.7839192955823875 4.2379651325656687E-5 92-93 6.356947698848502E-4 0.0 0.0 3.072567100761435 4.2379651325656687E-5 94-95 6.356947698848502E-4 0.0 0.0 3.357888103311419 4.2379651325656687E-5 96-97 6.356947698848502E-4 0.0 0.0 3.6489939282673545 4.2379651325656687E-5 98-99 6.356947698848502E-4 0.0 0.0 3.9672651097230363 4.2379651325656687E-5 100-101 6.356947698848502E-4 0.0 0.0 4.343278566109925 4.2379651325656687E-5 102-103 6.356947698848502E-4 0.0 0.0 4.753005035126375 4.2379651325656687E-5 104-105 6.78074421210507E-4 0.0 0.0 5.161163457043774 4.2379651325656687E-5 106-107 6.78074421210507E-4 0.0 0.0 5.5559934786192535 4.2379651325656687E-5 108-109 6.78074421210507E-4 0.0 0.0 5.977035314539653 8.475930265131337E-5 110-111 6.78074421210507E-4 0.0 0.0 6.450903385837483 8.475930265131337E-5 112-113 6.78074421210507E-4 0.0 0.0 6.971812870106792 8.475930265131337E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6995 0.0 51.916313 1 GTATCAA 11470 0.0 46.686058 1 ATCAACG 14680 0.0 36.681446 3 TCAACGC 14675 0.0 36.531586 4 CAACGCA 14685 0.0 36.38467 5 TATCAAC 14825 0.0 35.96106 2 AACGCAG 15085 0.0 35.419876 6 GTACATG 12470 0.0 31.369793 1 CGCAGAG 17205 0.0 30.57128 8 ACGCAGA 17325 0.0 30.393873 7 TACATGG 12915 0.0 29.61043 2 GCAGAGT 18360 0.0 28.128378 9 ACATGGG 13070 0.0 27.98317 3 GAGTACT 10160 0.0 27.640503 12-13 GTACTTT 10775 0.0 26.118093 14-15 AGTACTT 10620 0.0 25.546885 12-13 CAGAGTA 18185 0.0 24.013351 10-11 AGAGTAC 17920 0.0 23.256275 10-11 CATGGGA 8345 0.0 23.055807 4 CATGGGG 5065 0.0 21.992079 4 >>END_MODULE