FastQCFastQC Report
Thu 26 May 2016
SRR1364571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2999467
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.14399224928962379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.10365174879403574No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45150.052.051981
GTATCAA80300.052.013911
TCAACGC96050.042.741494
ATCAACG96150.042.635153
CAACGCA98600.041.756795
AACGCAG102250.040.2601666
TATCAAC105450.039.4392362
GTACATG132150.035.2977331
ACGCAGA118500.034.638857
TACATGG137550.033.0468942
CGCAGAG121250.033.0241178
ACATGGG138150.032.34353
GCAGAGT136250.028.1225389
ATGGGAG35800.026.7572065
CATGGGG68700.026.327814
CATGGGA87350.024.657194
GAGTACT59300.024.5379312-13
AGAGTAC122800.023.1396210-11
CAGAGTA128000.023.0131710-11
GAGTACA101350.022.0143281
Produced by FastQC (version 0.11.5)