FastQCFastQC Report
Thu 26 May 2016
SRR1364571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2999467
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85540.28518400102418195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75160.25057785266515686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47200.15736129118940131No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59300.063.536041
GTATCAA104550.050.6345181
ATCAACG135300.039.270783
TCAACGC136200.038.9675454
CAACGCA138500.038.1904345
TATCAAC141600.037.1028942
AACGCAG142250.037.0581786
ACGCAGA166050.031.7107857
GTACATG129950.031.4706421
CGCAGAG169700.030.9235528
TACATGG133550.029.7498232
ACATGGG136050.028.4150643
GCAGAGT185750.027.2901659
GAGTACT107950.026.0420112-13
GTACTTT114200.024.79911814-15
CATGGGG64750.024.7465654
AGTACTT111000.024.49562612-13
CAGAGTA179000.024.37988310-11
AGAGTAC173400.024.17221310-11
GTGGTAT26600.023.308241
Produced by FastQC (version 0.11.5)