##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364571_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2999467 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.98538807061388 31.0 30.0 34.0 26.0 34.0 2 29.865133705421663 31.0 30.0 34.0 26.0 34.0 3 30.169575127847715 31.0 30.0 34.0 26.0 34.0 4 33.73041443696497 35.0 35.0 37.0 28.0 37.0 5 33.13124031702966 35.0 33.0 37.0 26.0 37.0 6 33.14484873479188 35.0 33.0 37.0 26.0 37.0 7 32.9324620007488 35.0 33.0 37.0 26.0 37.0 8 32.85844118305019 35.0 33.0 37.0 25.0 37.0 9 34.09383867200406 38.0 34.0 39.0 25.0 39.0 10-11 34.03042857280977 38.0 34.0 39.0 25.0 39.0 12-13 33.89838878040665 38.0 33.5 39.0 24.0 39.0 14-15 34.8662115635878 39.0 34.0 41.0 24.5 41.0 16-17 34.86114382988711 39.0 34.0 41.0 24.0 41.0 18-19 34.863131849758645 39.0 34.0 41.0 24.0 41.0 20-21 34.875725753942284 39.0 34.0 41.0 24.0 41.0 22-23 34.83245706653882 39.0 34.0 41.0 24.0 41.0 24-25 34.78442786668431 39.0 34.0 41.0 24.0 41.0 26-27 34.667519429285264 39.0 34.0 41.0 23.5 41.0 28-29 34.51325035414625 39.0 33.0 41.0 23.0 41.0 30-31 34.348384229598125 38.0 33.0 40.5 22.5 41.0 32-33 34.156825696031994 38.0 33.0 40.0 21.5 41.0 34-35 33.97494421508888 38.0 33.0 40.0 19.0 41.0 36-37 33.77129086601053 38.0 33.0 40.0 18.0 41.0 38-39 33.60607934676394 38.0 33.0 40.0 16.0 41.0 40-41 33.63268707407016 38.0 33.0 40.0 16.0 41.0 42-43 33.499559921812775 38.0 33.0 40.0 15.0 41.0 44-45 33.33271511238497 38.0 32.0 40.0 14.0 41.0 46-47 33.0896345917458 38.0 32.0 40.0 9.0 41.0 48-49 32.8161870092253 38.0 32.0 40.0 7.5 41.0 50-51 32.64314543217178 38.0 31.0 40.0 6.0 41.0 52-53 32.44418374997958 37.5 31.0 40.0 2.0 41.0 54-55 32.23833067675024 37.0 31.0 40.0 2.0 41.0 56-57 32.01621888155462 37.0 31.0 40.0 2.0 41.0 58-59 31.79630397667319 36.5 30.5 40.0 2.0 41.0 60-61 31.542458043379042 36.0 30.0 40.0 2.0 41.0 62-63 31.362698772815303 36.0 30.0 39.5 2.0 41.0 64-65 31.22147651566095 35.5 30.0 39.0 2.0 41.0 66-67 30.91096151416235 35.0 30.0 39.0 2.0 41.0 68-69 30.621891322691667 35.0 30.0 38.5 2.0 40.5 70-71 30.304410416917406 35.0 29.0 37.5 2.0 40.0 72-73 30.00978207128133 35.0 29.0 37.0 2.0 39.5 74-75 29.666520751853582 35.0 29.0 36.5 2.0 39.0 76-77 29.364574939480914 34.5 29.0 36.0 2.0 39.0 78-79 29.090603430542828 34.0 29.0 36.0 2.0 38.0 80-81 28.82437879796644 34.0 29.0 35.0 2.0 37.0 82-83 28.557750593688812 34.0 29.0 35.0 2.0 37.0 84-85 28.35123073532731 34.0 28.5 35.0 2.0 36.0 86-87 28.146135463400665 34.0 28.0 35.0 2.0 36.0 88-89 27.983843629551515 34.0 28.0 35.0 2.0 36.0 90-91 27.85263515151192 34.0 27.0 35.0 2.0 35.0 92-93 27.74563197394737 34.0 27.0 35.0 2.0 35.0 94-95 27.64287071669733 34.0 27.0 35.0 2.0 35.0 96-97 27.530592268559715 34.0 27.0 35.0 2.0 35.0 98-99 27.445778199926856 34.0 27.0 35.0 2.0 35.0 100-101 27.347638263731525 34.0 27.0 35.0 2.0 35.0 102-103 26.069229466435203 31.5 24.5 34.0 2.0 35.0 104-105 27.005551819706636 33.5 25.5 35.0 2.0 35.0 106-107 27.17314392857131 34.0 26.0 35.0 2.0 35.0 108-109 27.17133694086316 34.0 27.0 35.0 2.0 35.0 110-111 27.094429276934868 34.0 26.0 35.0 2.0 35.0 112-113 27.013166005827035 34.0 25.5 35.0 2.0 35.0 114-115 26.908841470834652 34.0 25.0 35.0 2.0 35.0 116-117 26.783996790096374 34.0 25.0 35.0 2.0 35.0 118-119 26.643952242181694 34.0 25.0 35.0 2.0 35.0 120-121 26.45068407153671 34.0 25.0 35.0 2.0 35.0 122-123 26.149593744488605 34.0 24.5 35.0 2.0 35.0 124-125 24.333905157149587 31.5 12.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6266251915184142 1101 2 0.7318341611789911 1101 3 0.5771226324258905 1101 4 0.4446625439335037 1101 5 0.5538229455236561 1101 6 0.5890090301288708 1101 7 0.6685784943299637 1101 8 0.9051989693070297 1101 9 1.0466335247967322 1101 10-11 0.9424937370908353 1101 12-13 0.8347169236979823 1101 14-15 1.0989800884416852 1101 16-17 1.0931772604577716 1101 18-19 1.093270255275634 1101 20-21 1.0343496055801182 1101 22-23 1.1112466696759498 1101 24-25 1.1161253827703064 1101 26-27 1.1063012727902475 1101 28-29 1.2464465817751602 1101 30-31 1.2501366539806114 1101 32-33 1.2296122711564976 1101 34-35 1.272397719131341 1101 36-37 1.2876366014555032 1101 38-39 1.1877764039511192 1101 40-41 1.066922097971272 1101 42-43 1.137632999734663 1101 44-45 1.1467841991476035 1101 46-47 1.2806493249869533 1101 48-49 1.4027164261372747 1101 50-51 1.3434394994651058 1101 52-53 1.3727590642751863 1101 54-55 1.4269141066491002 1101 56-57 1.46688684160398 1101 58-59 1.4461457174848604 1101 60-61 1.3877550009436774 1101 62-63 1.2865889248657503 1101 64-65 1.227724451041972 1101 66-67 1.3492779241995052 1101 68-69 1.3245759440863623 1101 70-71 1.3510202794267698 1101 72-73 1.31642733150532 1101 74-75 1.380126080205219 1101 76-77 1.3864188931527366 1101 78-79 1.244251552176987 1101 80-81 1.1272029446122644 1101 82-83 1.2586745009082598 1101 84-85 1.3805802389101132 1101 86-87 1.4224917573109224 1101 88-89 1.383175522655268 1101 90-91 1.318350176346332 1101 92-93 1.2290253520037524 1101 94-95 1.308826639291329 1101 96-97 1.340733985421501 1101 98-99 1.2494961705217271 1101 100-101 1.3062892727986473 1101 102-103 1.2023065731020388 1101 104-105 1.2640433009242074 1101 106-107 1.2013764327257483 1101 108-109 1.1452648226066238 1101 110-111 1.1496964167759138 1101 112-113 1.1153893151204528 1101 114-115 1.2535637068661494 1101 116-117 1.2860946425694664 1101 118-119 1.2293339740996672 1101 120-121 1.1446922593298936 1101 122-123 1.147508075252599 1101 124-125 1.5752556449089958 1102 1 0.28153808044741524 1102 2 -0.4236720385884958 1102 3 0.21997692567764915 1102 4 0.18819172794961503 1102 5 0.4677783600013399 1102 6 0.251883709306604 1102 7 0.46111595350667045 1102 8 0.4542999374951364 1102 9 0.7432009749597057 1102 10-11 0.7689937957754651 1102 12-13 0.8665534193350766 1102 14-15 1.0105688499516532 1102 16-17 0.904763174518429 1102 18-19 1.013489655772844 1102 20-21 0.7902542772030614 1102 22-23 0.9270190636951128 1102 24-25 0.9584106238418499 1102 26-27 0.9579945165728105 1102 28-29 0.5665096190708638 1102 30-31 0.8741061200672604 1102 32-33 0.956409168885628 1102 34-35 1.048076593054688 1102 36-37 0.7340455427683139 1102 38-39 0.14381774811018744 1102 40-41 -0.2526548354556155 1102 42-43 0.01381501191440293 1102 44-45 -0.2716340387350513 1102 46-47 0.05173957962186648 1102 48-49 -0.32670983445898116 1102 50-51 -0.33246608374407316 1102 52-53 -0.4408644865252924 1102 54-55 -0.3317681612225343 1102 56-57 -0.4509808726423117 1102 58-59 -0.26418658437756903 1102 60-61 -0.32194198265667495 1102 62-63 -0.37101465273797984 1102 64-65 -0.34761299559791325 1102 66-67 -0.42620457130708544 1102 68-69 -0.1642401201963608 1102 70-71 -0.6658711068327996 1102 72-73 -0.5652573010069482 1102 74-75 -0.3371365158465167 1102 76-77 -0.6794512624543323 1102 78-79 -0.48085131444481277 1102 80-81 -0.4848142020634221 1102 82-83 -0.6795598712359556 1102 84-85 -0.6358134222652545 1102 86-87 -0.3430430784392975 1102 88-89 -0.3806838457472672 1102 90-91 -0.4936603145423568 1102 92-93 -0.4308866215658824 1102 94-95 -0.17441091460080216 1102 96-97 -0.4221902776085642 1102 98-99 -0.6534573487923048 1102 100-101 -0.4460523140627224 1102 102-103 -0.4527170946853438 1102 104-105 -0.38781517038087543 1102 106-107 -0.2563243555900918 1102 108-109 -0.25239427724019237 1102 110-111 -0.12275714225651413 1102 112-113 -0.0413395270608774 1102 114-115 -0.01510936012201114 1102 116-117 -0.12288173512184741 1102 118-119 0.07479369353334064 1102 120-121 0.2547123680571808 1102 122-123 0.21030147635422125 1102 124-125 0.43405078446394896 1103 1 0.327355019641157 1103 2 -0.1375715123240724 1103 3 0.20336696770927887 1103 4 0.1875252195869166 1103 5 0.3004317058246784 1103 6 0.36854167533570603 1103 7 0.445181777148953 1103 8 0.428049754183796 1103 9 0.626946420553459 1103 10-11 0.6030237375222427 1103 12-13 0.5654661588825718 1103 14-15 0.6357709738193478 1103 16-17 0.5711250148294553 1103 18-19 0.6620694857187104 1103 20-21 0.6165343426691479 1103 22-23 0.6807119700050066 1103 24-25 0.6212914236883762 1103 26-27 0.7034557684329101 1103 28-29 0.7425475868897564 1103 30-31 0.7282127703238288 1103 32-33 0.7578870459057327 1103 34-35 0.6934399741437289 1103 36-37 0.7904268650078023 1103 38-39 0.8542828263252176 1103 40-41 0.7598765149634019 1103 42-43 0.7285599220921029 1103 44-45 0.7417594957132394 1103 46-47 0.8328029333370921 1103 48-49 0.7515173850473147 1103 50-51 0.7238685935514511 1103 52-53 0.6754071448457637 1103 54-55 0.7341987350051653 1103 56-57 0.7779980930976507 1103 58-59 0.9129525943045422 1103 60-61 0.8793389569270502 1103 62-63 0.7893656892735912 1103 64-65 0.7798851692617887 1103 66-67 0.6802076972303652 1103 68-69 0.7732602915257125 1103 70-71 0.7349266762629902 1103 72-73 0.7410184683609877 1103 74-75 0.8178749196390953 1103 76-77 0.7292587547716138 1103 78-79 0.6689899305905804 1103 80-81 0.737805494538204 1103 82-83 0.8270221585472832 1103 84-85 0.8219799724052947 1103 86-87 0.8869778861953712 1103 88-89 0.8252289798438142 1103 90-91 0.8757568734784691 1103 92-93 0.8195117987185938 1103 94-95 0.6719446178746722 1103 96-97 0.7396101888510103 1103 98-99 0.7564222885030958 1103 100-101 0.676536096728583 1103 102-103 0.4781727834175591 1103 104-105 0.46951003168218364 1103 106-107 0.5964633398334733 1103 108-109 0.6451406400855078 1103 110-111 0.6079496880125426 1103 112-113 0.4909389008408347 1103 114-115 0.7130740318861548 1103 116-117 0.7117619638885913 1103 118-119 0.5724129771460511 1103 120-121 0.6902866045622993 1103 122-123 0.7144552326834663 1103 124-125 1.0546104569024273 1104 1 0.3655718428014332 1104 2 0.5435174314262916 1104 3 0.29597751356896396 1104 4 0.0974585658314382 1104 5 0.40743879171429853 1104 6 0.4841802960078425 1104 7 0.498485917174456 1104 8 0.4767089010805705 1104 9 0.6384054764083587 1104 10-11 0.597079663049918 1104 12-13 0.5323158382785138 1104 14-15 0.7106946181899474 1104 16-17 0.5910027652460101 1104 18-19 0.4882040750581851 1104 20-21 0.49018832556590297 1104 22-23 0.6492287279552684 1104 24-25 0.7197076895821866 1104 26-27 0.6493615529945203 1104 28-29 0.8016230360819208 1104 30-31 0.7906013556608329 1104 32-33 0.814940003472401 1104 34-35 0.9366772196324646 1104 36-37 0.9546170630674666 1104 38-39 1.0661061254780435 1104 40-41 1.0068116963593852 1104 42-43 0.9825760637276062 1104 44-45 0.9853738625063571 1104 46-47 0.9904845954635135 1104 48-49 0.9261097134335401 1104 50-51 0.9050791602851689 1104 52-53 0.9815883604366746 1104 54-55 0.44163607049301135 1104 56-57 0.7114016179649028 1104 58-59 0.8533368802738295 1104 60-61 0.8819900393573832 1104 62-63 0.919925283919266 1104 64-65 0.8444077497583393 1104 66-67 0.8644051604384657 1104 68-69 0.5397850441727172 1104 70-71 0.19741058349470464 1104 72-73 0.1409076805350793 1104 74-75 0.6058684039460758 1104 76-77 0.7186480974320908 1104 78-79 0.7482582550647727 1104 80-81 0.7189278869788858 1104 82-83 0.7403208023135655 1104 84-85 0.7357224974027048 1104 86-87 0.6928302789308702 1104 88-89 0.7469578121598452 1104 90-91 0.8276761941188404 1104 92-93 0.7714124682896113 1104 94-95 0.7022369642626138 1104 96-97 0.7646528398414887 1104 98-99 0.822608416411228 1104 100-101 0.7714560810341915 1104 102-103 0.7377355682255491 1104 104-105 0.7848348688491065 1104 106-107 0.7773068099163538 1104 108-109 0.7437121340660227 1104 110-111 0.790626721735098 1104 112-113 0.7490928544930924 1104 114-115 0.7750657073001008 1104 116-117 0.7524048953987368 1104 118-119 0.8491912203395025 1104 120-121 0.8504351609221317 1104 122-123 0.8636403729143858 1104 124-125 1.2827369709687453 1105 1 0.2920799837159649 1105 2 0.16283087281007624 1105 3 0.36110201687500876 1105 4 0.3663077604600815 1105 5 0.08256718812206998 1105 6 0.4748330543998094 1105 7 0.7024059668171247 1105 8 0.7787124701322128 1105 9 0.9244341075836644 1105 10-11 0.7687815532858266 1105 12-13 0.8431217131490456 1105 14-15 0.9791210990454005 1105 16-17 0.9735919929435184 1105 18-19 0.9009030972257932 1105 20-21 1.007472473609539 1105 22-23 1.0412126655351202 1105 24-25 0.9798755345872294 1105 26-27 0.9734730312873907 1105 28-29 1.0135749287088203 1105 30-31 1.0560602606528349 1105 32-33 1.183220605530451 1105 34-35 1.1401286343417496 1105 36-37 1.247736047958064 1105 38-39 1.2133160739281976 1105 40-41 1.0704699574143604 1105 42-43 1.0129592754577743 1105 44-45 1.108138071624424 1105 46-47 1.1508656187304211 1105 48-49 1.163990816869088 1105 50-51 0.9156312521803684 1105 52-53 1.026082448491664 1105 54-55 1.1206064435387972 1105 56-57 1.095662854092211 1105 58-59 0.7952156000284489 1105 60-61 0.889204935759814 1105 62-63 1.0860054536854982 1105 64-65 0.8392273084313686 1105 66-67 0.9775784574736903 1105 68-69 1.0651492770319564 1105 70-71 1.230602299626831 1105 72-73 1.2919478616205673 1105 74-75 1.133220959750485 1105 76-77 1.0950466182784702 1105 78-79 0.9988000966256614 1105 80-81 1.0361185611999666 1105 82-83 1.075524174684805 1105 84-85 1.1077523898979642 1105 86-87 1.1960866025488883 1105 88-89 1.1086497474195873 1105 90-91 0.92037892092155 1105 92-93 1.0387151113793855 1105 94-95 1.0002778114510598 1105 96-97 0.9982988590043291 1105 98-99 0.8938953565739212 1105 100-101 1.0425492943789827 1105 102-103 0.6739815970648806 1105 104-105 0.8986901619203422 1105 106-107 0.8742500358912189 1105 108-109 0.8428549343875957 1105 110-111 0.7870475435283701 1105 112-113 0.7814709995651441 1105 114-115 0.84390328450813 1105 116-117 0.573011316316169 1105 118-119 0.663296105113254 1105 120-121 0.7508311799443241 1105 122-123 0.7834246151913327 1105 124-125 1.2222774386922879 1106 1 -0.08731860726071261 1106 2 -0.5169883290197888 1106 3 0.0482218268553396 1106 4 -0.11946293521783247 1106 5 0.2206684234265026 1106 6 0.3492113615371011 1106 7 0.5313642715972193 1106 8 0.4736353293492712 1106 9 0.49766084086624574 1106 10-11 0.5246916905218271 1106 12-13 0.4843798812958795 1106 14-15 0.5388038538084601 1106 16-17 0.40790552309170636 1106 18-19 0.43921041495629964 1106 20-21 0.3976987899667961 1106 22-23 0.508324081342117 1106 24-25 0.5527310757206081 1106 26-27 0.6435043256408903 1106 28-29 0.7167041817022621 1106 30-31 0.6648318934529627 1106 32-33 0.5525672555201027 1106 34-35 0.5676491801991261 1106 36-37 0.6726522022020092 1106 38-39 0.5993450156864952 1106 40-41 0.6074554759777158 1106 42-43 0.3727716859349641 1106 44-45 0.4544568436680123 1106 46-47 0.47157755519499744 1106 48-49 0.6383249072712474 1106 50-51 0.6371588707958225 1106 52-53 0.5732657935150343 1106 54-55 0.5695884220759666 1106 56-57 0.13727975653667812 1106 58-59 0.5294167791669793 1106 60-61 0.4815836483051541 1106 62-63 0.4493159482641289 1106 64-65 0.5772921491493896 1106 66-67 0.444329478576023 1106 68-69 0.49559261914529174 1106 70-71 0.5748759396958896 1106 72-73 0.5897134048539208 1106 74-75 0.5227273025053307 1106 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-1.4677622482517947 2216 68-69 -1.305909294007538 2216 70-71 -1.4036262926423397 2216 72-73 -1.31090807044448 2216 74-75 -1.225438099975257 2216 76-77 -1.2357044447086132 2216 78-79 -1.2802199233582385 2216 80-81 -1.2607440911600811 2216 82-83 -1.1895668912405846 2216 84-85 -1.3007572201582498 2216 86-87 -1.4449808907948203 2216 88-89 -1.3588446272759462 2216 90-91 -1.3600178917925412 2216 92-93 -1.4080817942950645 2216 94-95 -1.3485270637268911 2216 96-97 -1.4162287314064912 2216 98-99 -1.349619928188055 2216 100-101 -1.4391655235192395 2216 102-103 -1.338431510345572 2216 104-105 -1.3026512122261984 2216 106-107 -1.3453263028625138 2216 108-109 -1.3326593546160161 2216 110-111 -1.336986489241447 2216 112-113 -1.3512577907387993 2216 114-115 -1.4858295962233896 2216 116-117 -1.4612005224689213 2216 118-119 -1.5046464252814324 2216 120-121 -1.660280310626046 2216 122-123 -1.577527695801443 2216 124-125 -1.7411009915835756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99663.0 3 33057.0 4 15896.0 5 12457.0 6 13482.0 7 15666.0 8 17493.0 9 19181.0 10 20684.0 11 22281.0 12 24235.0 13 27042.0 14 31088.0 15 27110.0 16 19333.0 17 17388.0 18 16230.0 19 15298.0 20 15305.0 21 15925.0 22 17796.0 23 22265.0 24 29278.0 25 38046.0 26 47130.0 27 53412.0 28 61260.0 29 72987.0 30 83988.0 31 98619.0 32 120600.0 33 149484.0 34 193105.0 35 267056.0 36 431270.0 37 628089.0 38 205546.0 39 722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67256856889995 19.101245833539128 13.721455488114993 24.504730109445934 2 15.674284797932433 20.276735833399734 38.407123665637926 25.641855703029904 3 19.110461958741336 24.53012485218207 29.335711978161456 27.023701210915142 4 13.15976539894794 16.783106161181095 35.61640178266897 34.44072665720199 5 13.759811326479005 38.00298519703668 33.540859092632125 14.696344383852198 6 32.976358799746755 36.3302546752473 16.585313324000563 14.108073201005379 7 28.8371956562037 32.407738337349684 20.272827535070302 18.482238471376313 8 26.771203040734843 35.560760517124976 18.777637929166215 18.89039851297397 9 26.60800970873786 15.838578896831324 19.132014829830364 38.42139656460045 10-11 25.562190661986474 26.577043554158852 26.52595084531345 21.33481493854122 12-13 26.375419366174057 23.51636140687662 27.427556295835227 22.680662931114096 14-15 23.021785048036058 24.757777677389523 25.340181442326976 26.880255832247446 16-17 22.99351316354049 28.385202131451038 25.670295876689764 22.950988828318707 18-19 22.883594796562782 26.362688295866977 28.313577173049826 22.440139734520415 20-21 23.436908740841993 25.654233728131224 28.367853838652408 22.541003692374375 22-23 23.18343559038989 25.542888119789282 28.44458698828825 22.829089301532573 24-25 23.092019348770965 25.77657963898253 28.293543486226053 22.837857526020457 26-27 22.88196536251274 26.173033408935652 28.123296572357688 22.821704656193916 28-29 22.697499255701096 26.108838670337096 28.43981947459332 22.753842599368486 30-31 22.731905368520476 25.769311681042 28.45593900516325 23.042843945274278 32-33 22.669477610522137 25.988283918442846 28.247051892886304 23.09518657814872 34-35 23.205489508636035 25.904952446551338 28.01559410388579 22.87396394092684 36-37 23.200288584605197 25.689230786669764 27.940347401721706 23.17013322700333 38-39 22.96266303313222 25.809102083803552 28.1772061502927 23.051028732771524 40-41 23.011371687036398 25.644172781364155 28.37225747107736 22.972198060522086 42-43 23.170833351392098 25.712918328489692 27.929812196633602 23.186436123484604 44-45 23.14577890005124 26.00225306696156 27.62565815859951 23.22630987438768 46-47 23.25453155510629 26.1530298549709 27.22435352680993 23.36808506311288 48-49 23.47412056875438 26.256248193429034 26.949004606485083 23.3206266313315 50-51 23.22207965983284 26.38549222003313 26.865727621895424 23.526700498238604 52-53 23.67493958093221 26.186269093809 26.798461193272004 23.340330131986782 54-55 23.24943064884528 26.183935345846443 26.883159574684434 23.68347443062384 56-57 23.275859050944987 26.63322581113024 26.78609861112477 23.3048165268 58-59 23.438291031361953 26.19446621234951 26.808996259809938 23.5582464964786 60-61 23.400207436854615 26.140044214522113 27.12278548155389 23.33696286706938 62-63 23.317759455263218 26.201005045229703 27.103048641641998 23.37818685786508 64-65 23.136332448952512 26.08437779841852 27.012377553041176 23.766912199587793 66-67 23.09590337216579 26.561885828382177 26.814997464549535 23.5272133349025 68-69 23.052520015229376 26.34445597983108 27.051631857137238 23.551392147802307 70-71 22.973781675210965 26.399990398294097 26.930918059775287 23.695309866719654 72-73 23.154047035690006 26.328361005471972 26.864939670948207 23.652652287889815 74-75 23.145584852052696 26.5304785460035 26.583255249039862 23.740681352903945 76-77 23.25769878448404 26.4332463067605 26.731916037082588 23.57713887167287 78-79 23.24202933387832 26.452199674142108 26.665587586061122 23.640183405918453 80-81 23.05950329792256 26.781051547876555 26.724124710281572 23.435320443919313 82-83 23.18311886745212 26.573621246708168 26.618762600155293 23.624497285684424 84-85 23.175984266538023 26.49105657771864 26.420510710736274 23.912448445007062 86-87 23.349731802350217 26.230343591044676 26.665470898662996 23.754453707942112 88-89 23.148577705001 26.32074655505437 26.58946423329362 23.941211506651015 90-91 23.076016505599164 26.596591994511027 26.76200471617124 23.565386783718573 92-93 23.206276980360098 26.322462729262213 26.68116732524195 23.790092965135738 94-95 23.057229834500596 26.264749703864055 26.584606531760475 24.093413929874874 96-97 23.306959179104243 26.223409052679347 26.543976641860702 23.92565512635571 98-99 22.965464367880855 26.24855739649054 26.48785912554764 24.29811911008096 100-101 22.634620956236958 26.262842972418177 26.90407057671143 24.19846549463343 102-103 23.1809518157726 26.03165829128975 26.662236990772026 24.125152902165617 104-105 23.039676715896523 26.045344056127306 26.75540354336287 24.159575684613298 106-107 22.940409755134773 26.15720910554371 26.800015536308536 24.102365603012977 108-109 23.099157800228806 26.221913130218322 26.390576457513358 24.288352612039507 110-111 22.792004887550497 26.194353478920775 26.759472337914442 24.25416929561429 112-113 22.872463674379482 26.182701793351953 26.690408662605726 24.254425869662843 114-115 22.682896661306824 26.360116647391017 26.467002304076026 24.48998438722613 116-117 22.5300695090161 26.315325356138274 26.67633949631718 24.478265638528445 118-119 22.64455651620771 26.487389259491767 26.21352393608598 24.65453028821454 120-121 22.394830368335423 26.48177659829835 26.571660043208066 24.55173299015816 122-123 22.577347119495784 26.364568691203655 26.436717993582583 24.621366195717986 124-125 22.500305323031768 26.455206274905517 26.260950752562877 24.783537649499838 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 397.0 1 314.5 2 454.5 3 690.5 4 509.0 5 358.0 6 764.0 7 1870.0 8 3207.5 9 3798.0 10 3016.5 11 1949.5 12 1647.5 13 1759.5 14 1947.5 15 2177.0 16 2495.0 17 2864.0 18 3296.0 19 3793.5 20 4385.0 21 5115.5 22 6087.5 23 7163.5 24 8622.0 25 10670.0 26 13316.0 27 16561.0 28 20026.0 29 23925.0 30 28496.0 31 33187.5 32 37664.5 33 41597.5 34 46579.5 35 50683.5 36 54403.5 37 60301.0 38 65512.0 39 67840.0 40 70609.0 41 78581.0 42 86831.0 43 92887.5 44 98627.0 45 103910.5 46 106649.5 47 110108.5 48 120064.5 49 125707.0 50 125391.0 51 123307.0 52 119122.5 53 119864.0 54 119387.5 55 111387.5 56 101405.0 57 91478.5 58 82669.5 59 73428.5 60 62074.5 61 50909.5 62 41470.0 63 32930.5 64 26312.0 65 21081.0 66 16156.0 67 13171.0 68 9720.0 69 6168.5 70 4336.0 71 3413.5 72 2764.5 73 2301.0 74 1842.5 75 1492.0 76 1090.0 77 627.5 78 359.0 79 214.0 80 120.5 81 74.0 82 52.5 83 36.5 84 28.0 85 23.0 86 15.5 87 8.5 88 5.5 89 2.0 90 1.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09731729003853018 2 0.0 3 0.0 4 0.10205146447685538 5 0.0 6 0.0 7 0.0021003731662992122 8 0.006401137268721409 9 0.003567300457047869 10-11 5.834369906386702E-4 12-13 0.0 14-15 5.000888491188601E-5 16-17 2.667140528633921E-4 18-19 0.0021670516795150607 20-21 0.0014002487775328083 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.6669628303962005E-5 52-53 0.0 54-55 0.0 56-57 0.008051430470813649 58-59 6.834547604624422E-4 60-61 0.0 62-63 0.0 64-65 1.6669628303962004E-4 66-67 0.0 68-69 3.333925660792401E-5 70-71 0.0 72-73 0.0 74-75 0.0012502221227971503 76-77 0.0 78-79 0.0 80-81 1.0001776982377202E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012168828661892263 90-91 0.0 92-93 0.0021003731662992122 94-95 0.0 96-97 0.008518180063324584 98-99 0.003850684138215223 100-101 0.0021337124229071367 102-103 0.0 104-105 0.0 106-107 0.0013835791492288463 108-109 1.6669628303962005E-5 110-111 3.167229377752781E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.001599284139481E-4 122-123 0.004367442615638045 124-125 0.08804897670152731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2999467.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.48641006879733 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39611815278147 33.65435635839302 2 13.140100260820692 12.21672178139243 3 5.355205042159419 7.468327727769415 4 2.796477201126246 5.199927436783893 5 1.6618698109721706 3.8627180756903514 6 1.0216128361867771 2.8494667940715357 7 0.7039674327722489 2.29074431284505 8 0.4920770948544316 1.8299918093492473 9 0.37977639251062106 1.5888997005027539 >10 1.841377109592746 15.154481214970794 >50 0.12592656834650642 4.044417794268223 >100 0.07944593292498743 6.892784916690507 >500 0.004678580022183951 1.5056023160019172 >1k 0.0012236286211273733 0.9038565984544052 >5k 1.439563083679263E-4 0.5377031628165065 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8554 0.28518400102418195 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7516 0.25057785266515686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4720 0.15736129118940131 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6669628303962005E-5 0.0 12-13 0.0 0.0 0.0 6.667851321584802E-5 0.0 14-15 0.0 0.0 0.0 6.667851321584802E-5 0.0 16-17 0.0 0.0 0.0 6.667851321584802E-5 0.0 18-19 0.0 0.0 0.0 6.667851321584802E-5 0.0 20-21 0.0 0.0 0.0 2.500444245594301E-4 0.0 22-23 0.0 0.0 0.0 5.334281057267842E-4 0.0 24-25 0.0 0.0 0.0 0.0010668562114535683 0.0 26-27 0.0 0.0 0.0 0.0014835969190526183 0.0 28-29 0.0 0.0 0.0 0.0021170427946031745 0.0 30-31 0.0 0.0 0.0 0.004150737447686539 0.0 32-33 0.0 0.0 0.0 0.008401492665196849 0.0 34-35 0.0 0.0 0.0 0.01415251443006374 0.0 36-37 0.0 0.0 0.0 0.022904069289643794 0.0 38-39 0.0 0.0 0.0 0.04032383086728409 0.0 40-41 0.0 0.0 0.0 0.058960475311113605 0.0 42-43 0.0 0.0 0.0 0.07764712863985501 0.0 44-45 0.0 0.0 0.0 0.10026781424833145 0.0 46-47 0.0 0.0 0.0 0.12727261210074992 0.0 48-49 0.0 0.0 0.0 0.15982839617838768 0.0 50-51 0.0 0.0 0.0 0.19713502432265467 0.0 52-53 0.0 0.0 0.0 0.23269134149500562 0.0 54-55 0.0 0.0 0.0 0.27058140662991126 0.0 56-57 0.0 0.0 0.0 0.3179398206414673 0.0 58-59 0.0 0.0 0.0 0.3712326223292338 0.0 60-61 0.0 0.0 0.0 0.43062650797625046 1.6669628303962005E-5 62-63 0.0 0.0 0.0 0.4920374186480465 3.333925660792401E-5 64-65 0.0 0.0 0.0 0.5586325837223747 3.333925660792401E-5 66-67 0.0 0.0 0.0 0.6328290993033096 3.333925660792401E-5 68-69 0.0 0.0 0.0 0.7215615307652994 3.333925660792401E-5 70-71 0.0 0.0 0.0 0.8222794249778378 3.333925660792401E-5 72-73 0.0 0.0 0.0 0.9288983676099787 3.333925660792401E-5 74-75 0.0 0.0 0.0 1.0339336955532432 3.333925660792401E-5 76-77 0.0 0.0 0.0 1.1525381009359328 3.333925660792401E-5 78-79 0.0 0.0 0.0 1.292012880955183 3.333925660792401E-5 80-81 0.0 0.0 0.0 1.4493741721445843 3.333925660792401E-5 82-83 0.0 0.0 0.0 1.6146702064066716 3.333925660792401E-5 84-85 0.0 0.0 0.0 1.7834835322408948 3.333925660792401E-5 86-87 0.0 0.0 0.0 1.9655658822050717 3.333925660792401E-5 88-89 0.0 0.0 0.0 2.170769006626844 3.333925660792401E-5 90-91 0.0 0.0 0.0 2.409994842417003 3.333925660792401E-5 92-93 0.0 0.0 0.0 2.6618729260898686 3.333925660792401E-5 94-95 0.0 0.0 0.0 2.921435708410861 3.333925660792401E-5 96-97 0.0 0.0 0.0 3.1875329850270067 3.333925660792401E-5 98-99 0.0 0.0 0.0 3.4714000854151754 3.333925660792401E-5 100-101 0.0 0.0 0.0 3.805126044060495 3.333925660792401E-5 102-103 0.0 0.0 0.0 4.16605683609788 3.333925660792401E-5 104-105 0.0 0.0 0.0 4.529104670929868 3.333925660792401E-5 106-107 0.0 0.0 0.0 4.9035878707783755 3.333925660792401E-5 108-109 0.0 0.0 0.0 5.292223585056945 3.333925660792401E-5 110-111 0.0 0.0 0.0 5.724500386235288 3.333925660792401E-5 112-113 0.0 0.0 0.0 6.204669029530914 3.333925660792401E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5930 0.0 63.53604 1 GTATCAA 10455 0.0 50.634518 1 ATCAACG 13530 0.0 39.27078 3 TCAACGC 13620 0.0 38.967545 4 CAACGCA 13850 0.0 38.190434 5 TATCAAC 14160 0.0 37.102894 2 AACGCAG 14225 0.0 37.058178 6 ACGCAGA 16605 0.0 31.710785 7 GTACATG 12995 0.0 31.470642 1 CGCAGAG 16970 0.0 30.923552 8 TACATGG 13355 0.0 29.749823 2 ACATGGG 13605 0.0 28.415064 3 GCAGAGT 18575 0.0 27.290165 9 GAGTACT 10795 0.0 26.04201 12-13 GTACTTT 11420 0.0 24.799118 14-15 CATGGGG 6475 0.0 24.746565 4 AGTACTT 11100 0.0 24.495626 12-13 CAGAGTA 17900 0.0 24.379883 10-11 AGAGTAC 17340 0.0 24.172213 10-11 GTGGTAT 2660 0.0 23.30824 1 >>END_MODULE