FastQCFastQC Report
Thu 26 May 2016
SRR1364570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558790
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.13479027196448323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26060.10184501268177537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.059.82231
GTATCAA69950.052.9029051
ATCAACG84550.043.5557443
TCAACGC85400.042.9132354
CAACGCA86650.042.431495
AACGCAG89800.040.872046
TATCAAC91450.040.1393052
GTACATG118500.036.6004181
ACGCAGA103150.035.294597
TACATGG122350.034.5727582
CGCAGAG104300.034.3411258
ACATGGG126100.032.4123153
GCAGAGT112550.030.1857159
GTATAGG9000.027.7641031
CATGGGA75750.026.7032834
CATGGGG54500.025.980584
GAGTACT52200.023.60002912-13
GAGTACA89550.023.5187661
AGAGTAC109200.023.3508510-11
ATGGGAT34850.023.2169155
Produced by FastQC (version 0.11.5)