##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364570_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2558790 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.8002094740092 31.0 28.0 33.0 15.0 34.0 2 27.708087807127587 31.0 28.0 33.0 12.0 34.0 3 27.892028654168573 31.0 28.0 34.0 16.0 34.0 4 31.193708745149074 35.0 32.0 37.0 16.0 37.0 5 31.270901089968305 35.0 32.0 37.0 16.0 37.0 6 31.370901089968307 35.0 32.0 37.0 16.0 37.0 7 31.071390383736063 35.0 32.0 37.0 11.0 37.0 8 31.034533900789043 35.0 32.0 37.0 11.0 37.0 9 32.06562164147898 37.0 32.0 39.0 10.0 39.0 10-11 31.97192950574295 37.0 31.5 39.0 10.0 39.0 12-13 31.884111826292894 37.0 31.0 39.0 8.0 39.0 14-15 32.53510800026575 38.0 31.0 40.0 8.0 41.0 16-17 32.64076751120647 38.0 31.0 40.0 8.0 41.0 18-19 32.43532216399157 38.0 31.0 40.0 8.0 41.0 20-21 32.16268880994532 38.0 30.0 40.0 7.0 41.0 22-23 32.05845126016594 38.0 30.0 40.0 2.0 41.0 24-25 31.87729688641897 38.0 30.0 40.0 2.0 41.0 26-27 31.788309513480982 38.0 30.0 40.0 2.0 41.0 28-29 31.84739955213206 38.0 30.0 40.0 2.0 41.0 30-31 31.78786828930862 37.5 30.0 40.0 2.0 41.0 32-33 31.76219052755404 37.0 30.0 40.0 2.0 41.0 34-35 31.277521602007198 37.0 29.5 40.0 2.0 41.0 36-37 31.13637363753962 37.0 29.5 40.0 2.0 41.0 38-39 30.911706314312624 37.0 29.0 40.0 2.0 41.0 40-41 31.114395475986697 37.0 29.5 40.0 2.0 41.0 42-43 30.978737997256516 37.0 29.0 40.0 2.0 41.0 44-45 30.809220178287394 37.0 29.0 40.0 2.0 41.0 46-47 30.757009172304095 37.0 29.0 40.0 2.0 41.0 48-49 30.597134387737952 36.5 28.5 40.0 2.0 41.0 50-51 30.43346132351619 36.0 28.0 40.0 2.0 41.0 52-53 30.360311123617024 36.0 28.0 40.0 2.0 41.0 54-55 30.377493072897735 36.0 28.0 40.0 2.0 41.0 56-57 30.241240195561183 36.0 28.0 40.0 2.0 41.0 58-59 30.03729106335416 36.0 28.0 40.0 2.0 41.0 60-61 29.826752683885744 35.0 28.0 39.5 2.0 41.0 62-63 29.539296894235164 35.0 28.0 39.0 2.0 41.0 64-65 29.251292016929877 35.0 27.0 39.0 2.0 40.5 66-67 29.002330593757208 35.0 26.5 38.5 2.0 40.0 68-69 28.968467322445374 35.0 27.5 38.0 2.0 40.0 70-71 28.74104928501362 34.5 27.0 37.5 2.0 40.0 72-73 28.463830951348097 34.0 26.5 37.0 2.0 39.5 74-75 28.165474110810187 34.0 26.0 36.5 2.0 39.0 76-77 27.867917062361506 34.0 26.0 36.0 2.0 39.0 78-79 27.57071389211307 34.0 26.0 36.0 2.0 38.5 80-81 27.28051403202295 34.0 26.0 35.0 2.0 37.0 82-83 27.038203799452084 34.0 26.0 35.0 2.0 37.0 84-85 26.796654082593726 34.0 25.0 35.0 2.0 36.5 86-87 26.607163346738105 33.0 25.0 35.0 2.0 36.0 88-89 26.410438918395023 33.0 24.5 35.0 2.0 36.0 90-91 26.27419366184798 33.0 24.0 35.0 2.0 35.5 92-93 26.1460156949183 33.0 24.0 35.0 2.0 35.0 94-95 26.015515732045223 33.0 24.0 35.0 2.0 35.0 96-97 25.902479687664872 33.0 23.0 35.0 2.0 35.0 98-99 25.80567279847115 33.0 23.0 35.0 2.0 35.0 100-101 25.708920622638047 33.0 21.5 35.0 2.0 35.0 102-103 24.56134422910829 31.0 19.0 34.0 2.0 35.0 104-105 25.417199926527772 33.0 20.0 35.0 2.0 35.0 106-107 25.561467138764808 33.0 20.0 35.0 2.0 35.0 108-109 25.51286213405555 33.0 19.5 35.0 2.0 35.0 110-111 25.419832420792638 33.0 19.0 35.0 2.0 35.0 112-113 25.319831248363485 33.0 18.5 35.0 2.0 35.0 114-115 25.18901941933492 33.0 18.0 35.0 2.0 35.0 116-117 25.074516666080452 33.0 16.0 35.0 2.0 35.0 118-119 24.91677277150528 33.0 5.0 35.0 2.0 35.0 120-121 24.72929529191532 33.0 2.0 35.0 2.0 35.0 122-123 24.48111314332165 33.0 2.0 35.0 2.0 35.0 124-125 23.059289156202738 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8741317960237005 1101 2 0.8849424303955828 1101 3 0.8763676978468098 1101 4 0.8987332739931055 1101 5 0.9628235011439301 1101 6 1.0009101655792918 1101 7 1.018935284783538 1101 8 1.041502265831415 1101 9 1.1499826853453285 1101 10-11 1.2293132373150897 1101 12-13 1.0015609283858602 1101 14-15 1.3240822760336428 1101 16-17 1.1016965744886917 1101 18-19 1.4647818235978463 1101 20-21 1.8213735828642896 1101 22-23 1.6666229845140919 1101 24-25 1.9058609916715952 1101 26-27 2.099613590195524 1101 28-29 1.854318757382245 1101 30-31 1.8518935783565915 1101 32-33 1.8309859432456044 1101 34-35 2.236919526098365 1101 36-37 2.0999665957580724 1101 38-39 1.9688478336968522 1101 40-41 1.7913805266350913 1101 42-43 1.811779861662334 1101 44-45 1.7796829630112256 1101 46-47 1.526303621815714 1101 48-49 1.4682954178630858 1101 50-51 1.3818260200220713 1101 52-53 1.3385150265080554 1101 54-55 1.222839670757807 1101 56-57 1.1777270015073 1101 58-59 1.2739232470966648 1101 60-61 1.1963253665524896 1101 62-63 1.2024151137457544 1101 64-65 1.3069149410623737 1101 66-67 1.1693036333204567 1101 68-69 1.008097325004094 1101 70-71 0.9533438893321673 1101 72-73 0.8032592997427699 1101 74-75 0.8905858696558084 1101 76-77 1.078544404459283 1101 78-79 1.1088990031293235 1101 80-81 1.2415522470107483 1101 82-83 1.0082278536891565 1101 84-85 1.0749615212803292 1101 86-87 1.1190398200470888 1101 88-89 1.0939093899338275 1101 90-91 1.0518450455134527 1101 92-93 0.947429850458164 1101 94-95 1.0145064609994812 1101 96-97 1.0069888711032284 1101 98-99 0.9841250262339294 1101 100-101 1.0345330633770864 1101 102-103 1.0898725974923167 1101 104-105 1.1138782990214864 1101 106-107 1.0316901686760325 1101 108-109 1.1012703306038567 1101 110-111 0.876608515267769 1101 112-113 0.9612770127145751 1101 114-115 1.1640527805624856 1101 116-117 1.1953008504742826 1101 118-119 1.117797426340914 1101 120-121 1.2102273099984622 1101 122-123 1.3936100652512415 1101 124-125 1.6643096346764352 1102 1 -0.23378500519499212 1102 2 0.041012188947792794 1102 3 0.06065891448814753 1102 4 0.10837395156463003 1102 5 0.29662879072673576 1102 6 -0.43135443617637037 1102 7 -1.1016224852888712 1102 8 -0.2113548281894424 1102 9 0.13518478743648643 1102 10-11 0.08438000761154285 1102 12-13 0.2878887753867083 1102 14-15 0.5425989377420137 1102 16-17 0.3302955975773969 1102 18-19 0.5141778156566659 1102 20-21 0.8332131725491152 1102 22-23 0.6715636926264565 1102 24-25 0.8503807758346085 1102 26-27 0.41219695241693444 1102 28-29 0.8576023212974917 1102 30-31 0.786224913398069 1102 32-33 0.5696647849991443 1102 34-35 0.9273227951264182 1102 36-37 1.1226423913384416 1102 38-39 1.0736887967305755 1102 40-41 0.8658358275329867 1102 42-43 0.7055362378746075 1102 44-45 0.730635534869073 1102 46-47 0.378862003269937 1102 48-49 0.22337371513647852 1102 50-51 0.47774798464837787 1102 52-53 0.1810832906498625 1102 54-55 0.21449751561430475 1102 56-57 0.20573896809150227 1102 58-59 0.4119077297515332 1102 60-61 0.2045134755151956 1102 62-63 0.16198155815083481 1102 64-65 0.029649473370824353 1102 66-67 0.13819788828079993 1102 68-69 -0.009231704924513195 1102 70-71 0.04665180116096579 1102 72-73 0.19567877264914912 1102 74-75 -0.07938621415322444 1102 76-77 -0.007940200952237575 1102 78-79 -0.06104402309200552 1102 80-81 -0.4115815227090742 1102 82-83 -0.19621516024238161 1102 84-85 -0.5594471264385845 1102 86-87 -0.03341553905073269 1102 88-89 0.18213907512022232 1102 90-91 -0.3077329258685708 1102 92-93 0.03787191002381718 1102 94-95 -0.056907822789600715 1102 96-97 0.1463271378696831 1102 98-99 0.22088192822326747 1102 100-101 0.1732818439331112 1102 102-103 0.034309743401525594 1102 104-105 0.31120870201646866 1102 106-107 0.25204528432297124 1102 108-109 -0.17918214844487323 1102 110-111 0.12200761282978334 1102 112-113 -0.25173361046826415 1102 114-115 -0.15955636112129667 1102 116-117 -0.2200525036358627 1102 118-119 0.1073244049761719 1102 120-121 0.5443223278864657 1102 122-123 0.5031536393430045 1102 124-125 0.7038932876149815 1103 1 0.574851299175954 1103 2 0.5658576153889641 1103 3 0.49755739002572597 1103 4 0.6377871038701919 1103 5 0.7386032320488312 1103 6 0.7195473572886542 1103 7 0.7474757212461078 1103 8 0.8602049236693858 1103 9 1.1135337668392609 1103 10-11 1.1502757793985197 1103 12-13 1.0661241835398165 1103 14-15 1.4147916091368131 1103 16-17 1.2636168789767552 1103 18-19 1.2769648747102806 1103 20-21 1.700944269455242 1103 22-23 1.700110049253908 1103 24-25 1.7205376997350044 1103 26-27 1.659389838258953 1103 28-29 1.6309423282284286 1103 30-31 1.5079108031138624 1103 32-33 1.4936400274960668 1103 34-35 1.869401243352005 1103 36-37 1.862767096687957 1103 38-39 1.7886772852036081 1103 40-41 1.4476864727617915 1103 42-43 1.307306885711096 1103 44-45 0.9835919042947339 1103 46-47 1.093293357855849 1103 48-49 1.2051731503682674 1103 50-51 1.1576516206439464 1103 52-53 1.1144802719191844 1103 54-55 1.0002299987072831 1103 56-57 1.0005217673654698 1103 58-59 0.855756654255817 1103 60-61 0.8527863604651174 1103 62-63 0.8208950998925602 1103 64-65 0.9668685475078007 1103 66-67 1.029859150877428 1103 68-69 1.0793356417971864 1103 70-71 1.0323734761138716 1103 72-73 1.0450407492692548 1103 74-75 1.0766389379327492 1103 76-77 1.0475345079999911 1103 78-79 0.9961466925441158 1103 80-81 0.9581297798605171 1103 82-83 0.992834220948712 1103 84-85 0.9606708109565503 1103 86-87 0.9615123552147793 1103 88-89 0.9645810800130512 1103 90-91 1.0311029087771821 1103 92-93 1.1360085352078109 1103 94-95 0.9788924256260607 1103 96-97 1.1451817752480054 1103 98-99 1.0316822195283741 1103 100-101 0.9301124169017818 1103 102-103 0.8210636386208989 1103 104-105 0.8090781688492044 1103 106-107 0.8538324445956782 1103 108-109 0.9862840426636303 1103 110-111 1.0517894428022991 1103 112-113 1.1462911590438587 1103 114-115 1.2262183251853038 1103 116-117 1.1592703234990864 1103 118-119 1.1080129950114106 1103 120-121 1.145888178624336 1103 122-123 1.1315862960524576 1103 124-125 1.1760007805725365 1104 1 0.9142122857529031 1104 2 0.9035923323168227 1104 3 0.788833472274213 1104 4 0.9833874517666992 1104 5 0.8432201196803888 1104 6 0.8612254784344202 1104 7 0.864247409503136 1104 8 0.8536487660877192 1104 9 1.1378093376319356 1104 10-11 1.0984685689371148 1104 12-13 1.1456427091618941 1104 14-15 1.1401216246669605 1104 16-17 1.2209479120908568 1104 18-19 1.468087120640618 1104 20-21 1.8344930402298019 1104 22-23 1.6320396761309084 1104 24-25 1.9237336991915868 1104 26-27 1.9363367001312568 1104 28-29 1.8218676503523596 1104 30-31 1.7264645619145007 1104 32-33 1.6699659833457012 1104 34-35 2.083675688675509 1104 36-37 2.107776606569441 1104 38-39 2.222144609304099 1104 40-41 1.846383925338916 1104 42-43 1.7788508962172465 1104 44-45 1.6270781016474132 1104 46-47 1.5628944356424128 1104 48-49 1.4032103055018688 1104 50-51 1.1586853873828034 1104 52-53 1.295342900748775 1104 54-55 1.1184831773252348 1104 56-57 1.1649498492197097 1104 58-59 1.2340681641426308 1104 60-61 1.1740004886327462 1104 62-63 1.244339688183949 1104 64-65 1.2211050065820146 1104 66-67 1.2803873888322421 1104 68-69 1.3026212991910526 1104 70-71 1.26330956012049 1104 72-73 1.217581299457187 1104 74-75 1.2467212703310757 1104 76-77 1.2706214435006196 1104 78-79 1.3123418275870975 1104 80-81 1.3012225957710584 1104 82-83 1.2452129566406 1104 84-85 1.2915696346081837 1104 86-87 1.3070079479041574 1104 88-89 1.3535924385941875 1104 90-91 1.4416196189975068 1104 92-93 1.4548742620744264 1104 94-95 1.4395599420552472 1104 96-97 1.2331442165437991 1104 98-99 1.3758497253022313 1104 100-101 1.4217064275411282 1104 102-103 1.4040683549360722 1104 104-105 1.4278889208757128 1104 106-107 1.4168261859215754 1104 108-109 1.5271307817332627 1104 110-111 1.5574532165470352 1104 112-113 1.5245247851323676 1104 114-115 1.5565762243805636 1104 116-117 1.616387817152507 1104 118-119 1.5200052019698695 1104 120-121 1.5319102178412756 1104 122-123 1.655711260423697 1104 124-125 1.9373151087939746 1105 1 0.1296812429570231 1105 2 0.35783807466724227 1105 3 0.5000202314366611 1105 4 0.42646910762170975 1105 5 0.38493440166638493 1105 6 0.3642324612896424 1105 7 0.5518278444594955 1105 8 0.20897121545345598 1105 9 0.2956707994806891 1105 10-11 0.5006785830789866 1105 12-13 0.5429583632520298 1105 14-15 0.6294132750903145 1105 16-17 0.6242628517473108 1105 18-19 0.646831903072723 1105 20-21 0.9560971558897648 1105 22-23 1.2682836470772756 1105 24-25 1.3140620940156893 1105 26-27 1.124609385439534 1105 28-29 0.9283425725883774 1105 30-31 0.7756786033092418 1105 32-33 0.9085731312709946 1105 34-35 1.3585824289821282 1105 36-37 1.3697246943175507 1105 38-39 1.4966929909813196 1105 40-41 1.148659673903932 1105 42-43 0.8013080913636657 1105 44-45 0.9898892187574511 1105 46-47 0.9326306660750099 1105 48-49 0.860364184403732 1105 50-51 0.8263107762027317 1105 52-53 0.7908103246933962 1105 54-55 0.637207538045736 1105 56-57 0.6419710196186657 1105 58-59 0.6865865428675129 1105 60-61 0.7790745988936756 1105 62-63 0.7798083844077262 1105 64-65 0.8053011789791 1105 66-67 0.6876017009940902 1105 68-69 0.47517106080182714 1105 70-71 0.6344510065713997 1105 72-73 0.6778976076292977 1105 74-75 0.7855248059622326 1105 76-77 0.6526389193966402 1105 78-79 0.7362455062681015 1105 80-81 0.5851662696891715 1105 82-83 0.5043264716706197 1105 84-85 0.5411550836493291 1105 86-87 0.5231427312007604 1105 88-89 0.6315415572146428 1105 90-91 0.5302566981055392 1105 92-93 0.48353055585999627 1105 94-95 0.5295971806875137 1105 96-97 0.6320329296334322 1105 98-99 0.5123396702190703 1105 100-101 0.6095762244494907 1105 102-103 0.5537704295666614 1105 104-105 0.6887366502651986 1105 106-107 0.7148696489349504 1105 108-109 0.5913774538261443 1105 110-111 0.72745026420694 1105 112-113 0.7482784001178615 1105 114-115 0.6930454539829078 1105 116-117 0.7299844035296594 1105 118-119 0.8136568551987686 1105 120-121 0.6628259489206094 1105 122-123 0.8604718680511745 1105 124-125 0.9483429961744321 1106 1 0.4497087579237231 1106 2 0.7595813586889797 1106 3 0.4935742981788671 1106 4 0.687431160733631 1106 5 0.47423519682214277 1106 6 0.5777914485915865 1106 7 0.27541155171631715 1106 8 0.6034407740926362 1106 9 0.7158213284408603 1106 10-11 0.8320461472178167 1106 12-13 0.8227251554989614 1106 14-15 1.1163217409635564 1106 16-17 1.0049190267987242 1106 18-19 0.9952128882892879 1106 20-21 1.3120993182581913 1106 22-23 1.3120724446029328 1106 24-25 1.488224636505734 1106 26-27 1.7555166931402013 1106 28-29 1.6643838316491468 1106 30-31 1.4099643606319283 1106 32-33 1.3193628811090683 1106 34-35 1.5675490612271865 1106 36-37 1.4558781722662495 1106 38-39 1.7273375340973054 1106 40-41 1.3156564420669064 1106 42-43 1.3184667861098767 1106 44-45 1.1766539172560115 1106 46-47 1.0329222176078297 1106 48-49 1.0589563018009223 1106 50-51 1.014683359647222 1106 52-53 0.8199489087437541 1106 54-55 0.7493380567296342 1106 56-57 0.8340485837072826 1106 58-59 0.7562057685320802 1106 60-61 0.8076143474765054 1106 62-63 0.8134730465878697 1106 64-65 0.8110294469253354 1106 66-67 0.7644810953970413 1106 68-69 0.7234044292262283 1106 70-71 0.8437501950137793 1106 72-73 0.8559333335708565 1106 74-75 0.7988332463958443 1106 76-77 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86-87 -2.4023221979106957 2216 88-89 -2.572190971656898 2216 90-91 -2.5395349797775886 2216 92-93 -2.430488137632672 2216 94-95 -2.487184793656585 2216 96-97 -2.4925055898631356 2216 98-99 -2.538257550115553 2216 100-101 -2.573942863232169 2216 102-103 -2.362881545350753 2216 104-105 -2.6053173092461073 2216 106-107 -2.59914831700328 2216 108-109 -2.6883567210645545 2216 110-111 -2.6665431918774374 2216 112-113 -2.6572122203958592 2216 114-115 -2.730981478336197 2216 116-117 -2.7277651134469494 2216 118-119 -2.7427132185665215 2216 120-121 -2.7807761556324557 2216 122-123 -2.814421712561419 2216 124-125 -2.878327170514112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 194745.0 3 32237.0 4 19608.0 5 15594.0 6 14166.0 7 15526.0 8 16033.0 9 16758.0 10 17864.0 11 19036.0 12 20558.0 13 22358.0 14 24467.0 15 21936.0 16 17252.0 17 15096.0 18 13904.0 19 13108.0 20 13174.0 21 14114.0 22 16785.0 23 21740.0 24 28762.0 25 37205.0 26 44588.0 27 51097.0 28 58663.0 29 68134.0 30 76803.0 31 87575.0 32 105895.0 33 131940.0 34 168851.0 35 227772.0 36 340583.0 37 419914.0 38 134582.0 39 367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.566093001931236 18.969631376338057 12.96805933875143 23.496216282979272 2 15.100410019467228 20.300712209012612 40.72918665597878 23.869691115541382 3 19.295945132043997 25.755546340370966 28.43578408171098 26.51272444587406 4 12.14705616811154 16.44372460268109 37.73047389047158 33.678745338735794 5 13.405674743513977 38.63151690259316 33.83864441546827 14.12416393842459 6 31.511789094965025 36.986700214126905 17.749396876537606 13.752113814370459 7 28.63831801306421 32.534834575096546 20.9504207976806 17.876426614158643 8 26.301963037216808 35.554773936118245 19.65643917632944 18.48682385033551 9 26.91592189207399 16.331706996790576 19.73517185963585 37.017199251499584 10-11 24.953962615142313 27.070158160693143 27.27632982777016 20.69954939639439 12-13 25.739864028422282 24.476838108779216 27.506349424404185 22.276948438394314 14-15 22.703405661339822 25.877682788446542 25.315649187213847 26.10326236299979 16-17 22.462608643981593 29.15741957325438 25.886460222414993 22.493511560349035 18-19 22.333062486003293 27.69327966413368 28.117353106360927 21.856304743502104 20-21 23.158158040788248 26.850323226164512 28.27193208732969 21.719586645717545 22-23 22.90557234766147 26.869238868162558 27.861406112847643 22.363782671328334 24-25 22.493152746278835 27.054483912984423 28.090048592783095 22.36231474795364 26-27 22.28531089346692 27.22096357586901 28.01547431423318 22.478251216430888 28-29 22.284324336788043 27.220370969976493 28.12855821635206 22.366746476883403 30-31 22.415932826378583 27.08949806399042 27.96617869910708 22.52839041052392 32-33 22.22875801793939 27.183831370738105 27.78217586888247 22.805234742440035 34-35 22.715836877345332 27.01924651588542 27.989897367288624 22.275019239480624 36-37 22.870119210474886 26.71160836427594 27.785772913816693 22.63249951143248 38-39 22.489540464348472 27.046871186551297 27.91099072432492 22.55259762477531 40-41 22.747385676420983 26.937808778279287 27.726010015376247 22.588795529923484 42-43 22.497874275538837 27.066085944735267 27.52333899867483 22.912700781051065 44-45 22.608956010301956 27.134335464004323 27.586208918329753 22.67049960736397 46-47 22.877194463480926 27.4047962555744 27.068158293122224 22.649850987822447 48-49 22.736032560852237 27.441985736645503 26.950011805341646 22.87196989716062 50-51 22.73127338372147 27.340951611207537 27.02199696639388 22.90577803867711 52-53 23.027094837794426 27.36299188288214 26.898827180034313 22.71108609928912 54-55 22.759141497232015 27.27095589715447 27.080931893505255 22.888970712108257 56-57 22.648343041524797 27.46689287847095 26.929972047912504 22.95479203209175 58-59 22.861013560358014 27.13203000490077 26.9438154559474 23.063140978793818 60-61 22.769371948126256 27.32823720411432 27.11803066744728 22.784360180312145 62-63 22.66064320218036 27.327414373206437 27.189617166131047 22.822325258482152 64-65 22.507036258252615 27.190044542285076 27.135145995151966 23.167773204310347 66-67 22.512528661646215 27.489699003373936 26.893544578508195 23.10422775647166 68-69 22.68777303842317 27.127686604269797 27.283144558307093 22.90139579899994 70-71 22.522812294329793 27.118608297625208 27.275562510454492 23.083016897590504 72-73 22.681372791389265 27.372790216693073 26.89496754030371 23.050869451613956 74-75 22.58474614358589 27.55277985015568 26.921719610331294 22.940754395927136 76-77 22.825963758701928 27.22720926996812 27.07445162016518 22.872375351164763 78-79 22.769950528781415 27.33335144105906 26.933411704612446 22.963286325547077 80-81 22.587820561047174 27.503993731185496 26.96887772001184 22.939307987755488 82-83 22.83157113925515 27.629143540067737 26.823005211154545 22.71628010952257 84-85 22.704500955529763 27.534850456661157 26.8057753860223 22.954873201786782 86-87 22.6345769439489 27.411746520659324 26.991874780278653 22.96180175511312 88-89 22.773267384590188 27.473678841112665 26.73513656553314 23.017917208764008 90-91 22.739823162440654 27.65890079580615 26.852364541252943 22.74891150050025 92-93 22.754952289572884 27.50539832295357 26.913859375885473 22.825790011588072 94-95 22.55222271476027 27.23751044688837 27.108805563959976 23.101461274391383 96-97 22.833304212785603 27.17893032606023 26.935724892517815 23.05204056863635 98-99 22.475306901405233 27.339046644245663 26.74278161524628 23.44286483910282 100-101 22.500398660500718 27.195544538976492 27.02990500936461 23.27415179115818 102-103 22.55265183934594 27.102927555602452 27.070119079721273 23.274301525330333 104-105 22.381918347973627 27.062839509236646 27.126913801805845 23.428328340983885 106-107 22.419042758117218 27.248319419850564 27.063876701048816 23.268761120983402 108-109 22.44644929830115 27.162740983042767 26.77966929681607 23.61114042184001 110-111 22.324964729147208 27.20648983691383 27.04449794237075 23.424047491568214 112-113 22.420538613954253 27.29006678938092 27.127978458568307 23.16141613809652 114-115 22.371355210861385 27.418271917586047 26.954068133766352 23.25630473778622 116-117 22.13348887560136 27.434295116050944 27.060563782100132 23.371652226247562 118-119 22.131085395831622 27.42772951277752 26.955768156042502 23.48541693534835 120-121 22.188879620179627 27.529004448785038 27.077149987191202 23.204965943844126 122-123 22.22095209063659 27.53973557814436 26.972983324149304 23.266329007069746 124-125 22.221601120328828 27.38250180059828 26.93313892409733 23.462758154975564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 651.0 1 661.5 2 758.5 3 814.0 4 706.0 5 580.0 6 553.5 7 653.5 8 865.0 9 1026.5 10 1027.0 11 1057.5 12 1173.0 13 1282.5 14 1424.0 15 1581.0 16 1780.0 17 1966.5 18 2183.5 19 2489.0 20 2826.0 21 3440.5 22 4314.5 23 5522.0 24 7108.0 25 9313.0 26 12682.0 27 16894.0 28 20587.5 29 24693.5 30 30455.5 31 36491.5 32 43655.5 33 50140.5 34 55461.0 35 60404.5 36 65607.5 37 71053.5 38 73738.0 39 75721.5 40 76952.0 41 77816.0 42 79035.5 43 80977.5 44 83453.5 45 85971.0 46 86756.0 47 85904.5 48 87386.5 49 87862.5 50 86109.5 51 84968.0 52 85099.0 53 85844.0 54 85964.0 55 82849.0 56 77310.5 57 73280.0 58 70420.5 59 63677.0 60 53539.0 61 43864.5 62 35638.0 63 27412.0 64 19754.0 65 14684.0 66 10921.5 67 8220.0 68 6044.5 69 4388.0 70 3307.5 71 2432.5 72 1815.0 73 1511.5 74 1226.5 75 959.0 76 710.5 77 452.0 78 312.0 79 213.0 80 156.5 81 129.0 82 98.0 83 71.5 84 53.0 85 40.0 86 32.5 87 27.5 88 24.5 89 18.5 90 13.5 91 10.0 92 9.5 93 8.5 94 4.0 95 5.0 96 6.5 97 3.5 98 3.0 99 6.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012779477800053932 2 0.005119607314394694 3 0.008246085063643363 4 0.007933437288718495 5 0.014303635702812657 6 0.0010551862403714255 7 0.007698951457524846 8 0.0 9 0.0028919919180550183 10-11 0.0 12-13 0.003654070869434381 14-15 0.0191887571860137 16-17 0.03044407708330891 18-19 0.005979388695438078 20-21 0.008207004091777754 22-23 0.0018758866495492009 24-25 0.01104037455203436 26-27 0.01899335232668566 28-29 4.494311764544961E-4 30-31 0.001856346163616397 32-33 0.009008164015022725 34-35 0.00973116199453648 36-37 0.011333481841026422 38-39 1.9540485932804177E-5 40-41 0.02436698595820681 42-43 0.009789783452334893 44-45 0.017234708592733284 46-47 0.00416212350368729 48-49 0.024464688387870828 50-51 0.004650635652007394 52-53 0.0 54-55 0.016765736930345984 56-57 0.004924202455066653 58-59 3.1264777492486683E-4 60-61 0.004533392736410569 62-63 0.026692303784210506 64-65 0.01698068227560683 66-67 0.002227615396339676 68-69 0.02118188675115973 70-71 0.003204639692979885 72-73 0.012095560792405785 74-75 3.9080971865608354E-5 76-77 0.006331117442228554 78-79 0.0011528886700354465 80-81 0.003614989897568773 82-83 0.0018758866495492009 84-85 0.0 86-87 0.005920767237639665 88-89 0.017176087134934872 90-91 0.022139370561867135 92-93 0.004885121483201044 94-95 0.023917554781752313 96-97 0.017938166086314232 98-99 0.001113807698169838 100-101 0.008675975754165054 102-103 0.0 104-105 0.0016218603324227468 106-107 3.9080971865608354E-5 108-109 0.0 110-111 7.816194373121671E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.862145779841253E-5 122-123 0.0 124-125 0.02393709526768512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2558790.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.09739965241664 #Duplication Level Percentage of deduplicated Percentage of total 1 77.57500152835654 41.966058607163355 2 11.29251050727195 12.217909079820098 3 4.395209964177054 7.133082899651098 4 2.066856283124898 4.472462014892641 5 1.2236864110728414 3.3099126414519455 6 0.7713683310818975 2.5037412531453027 7 0.5100461401982811 1.9314518921235257 8 0.36189848273107433 1.5662213483124905 9 0.26515751545781824 1.2909898876107095 >10 1.3833037766540206 13.707346438614934 >50 0.10374992538525632 3.884990771295873 >100 0.0484695819794873 4.653738194828271 >500 0.0019264963576764488 0.6639516524282265 >1k 8.150561513220634E-4 0.6981433186617082 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3449 0.13479027196448323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2606 0.10184501268177537 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.1724291559682506E-4 0.0 0.0 0.0 0.0 8 1.1724291559682506E-4 0.0 0.0 0.0 0.0 9 1.1724291559682506E-4 0.0 0.0 0.0 0.0 10-11 1.1724291559682506E-4 0.0 0.0 0.0 0.0 12-13 1.9540485932804176E-4 0.0 0.0 3.9080971865608354E-5 0.0 14-15 1.9540485932804176E-4 0.0 0.0 1.3678340152962923E-4 0.0 16-17 1.9540485932804176E-4 0.0 0.0 1.5632388746243342E-4 0.0 18-19 2.7356680305925846E-4 0.0 0.0 1.5632388746243342E-4 0.0 20-21 3.517287467904752E-4 0.0 0.0 1.758643733952376E-4 0.0 22-23 3.517287467904752E-4 0.0 0.0 2.7356680305925846E-4 0.0 24-25 4.885121483201044E-4 3.9080971865608354E-5 0.0 4.4943117645449606E-4 0.0 26-27 5.080526342529086E-4 3.9080971865608354E-5 0.0 8.207004091777754E-4 0.0 28-29 6.448360357825379E-4 3.9080971865608354E-5 0.0 0.001426455473094705 1.9540485932804177E-5 30-31 7.425384654465587E-4 3.9080971865608354E-5 0.0 0.003106937263315864 3.9080971865608354E-5 32-33 7.425384654465587E-4 3.9080971865608354E-5 0.0 0.007210439309204742 3.9080971865608354E-5 34-35 8.207004091777754E-4 3.9080971865608354E-5 0.0 0.013248449462441232 3.9080971865608354E-5 36-37 9.770242966402089E-4 3.9080971865608354E-5 0.0 0.024210662070744376 3.9080971865608354E-5 38-39 0.001211510127833859 3.9080971865608354E-5 0.0 0.0416212350368729 3.9080971865608354E-5 40-41 0.0012310506137666632 3.9080971865608354E-5 0.0 0.06331117442228554 3.9080971865608354E-5 42-43 0.0013287530434306842 3.9080971865608354E-5 0.0 0.0851769781810934 3.9080971865608354E-5 44-45 0.0014069149871619008 3.9080971865608354E-5 0.0 0.11114628398579016 3.9080971865608354E-5 46-47 0.0015241579027587258 3.9080971865608354E-5 0.0 0.1436030311201779 3.9080971865608354E-5 48-49 0.0015632388746243341 3.9080971865608354E-5 0.0 0.178717284341427 3.9080971865608354E-5 50-51 0.0015632388746243341 3.9080971865608354E-5 0.0 0.219830466744047 7.816194373121671E-5 52-53 0.0015632388746243341 3.9080971865608354E-5 0.0 0.25861833132066325 7.816194373121671E-5 54-55 0.0015632388746243341 3.9080971865608354E-5 0.0 0.3003372687872002 7.816194373121671E-5 56-57 0.001641400818355551 3.9080971865608354E-5 0.0 0.3504390747189101 7.816194373121671E-5 58-59 0.001641400818355551 3.9080971865608354E-5 0.0 0.4094318017500459 7.816194373121671E-5 60-61 0.001641400818355551 3.9080971865608354E-5 0.0 0.4777648810570621 7.816194373121671E-5 62-63 0.0016609413042883551 3.9080971865608354E-5 0.0 0.547426713407509 7.816194373121671E-5 64-65 0.0017195627620867676 3.9080971865608354E-5 0.0 0.6174598149906791 7.816194373121671E-5 66-67 0.0018563461636163967 3.9080971865608354E-5 0.0 0.696911430793461 7.816194373121671E-5 68-69 0.0018758866495492009 3.9080971865608354E-5 0.0 0.7919954353424861 7.816194373121671E-5 70-71 0.0019345081073476136 3.9080971865608354E-5 0.0 0.9019106687145095 7.816194373121671E-5 72-73 0.0020517510229444385 3.9080971865608354E-5 0.0 1.0177075883523072 7.816194373121671E-5 74-75 0.002129912966675655 3.9080971865608354E-5 0.0 1.1340125606243576 7.816194373121671E-5 76-77 0.0021494534526084593 3.9080971865608354E-5 0.0 1.2640740349931021 7.816194373121671E-5 78-79 0.0021494534526084593 3.9080971865608354E-5 0.0 1.410275950742343 7.816194373121671E-5 80-81 0.0023448583119365014 3.9080971865608354E-5 0.0 1.5775815912990123 7.816194373121671E-5 82-83 0.0023839392838021097 3.9080971865608354E-5 0.0 1.7601874323410676 7.816194373121671E-5 84-85 0.002403479769734914 3.9080971865608354E-5 0.0 1.9471508017461376 7.816194373121671E-5 86-87 0.002442560741600522 3.9080971865608354E-5 0.0 2.1375923776472474 7.816194373121671E-5 88-89 0.0024621012275333263 3.9080971865608354E-5 0.0 2.3544136095576427 7.816194373121671E-5 90-91 0.0025793441431301513 3.9080971865608354E-5 0.0 2.6057237991394366 7.816194373121671E-5 92-93 0.0026379656009285638 3.9080971865608354E-5 0.0 2.8750893977231424 7.816194373121671E-5 94-95 0.002657506086861368 3.9080971865608354E-5 0.0 3.1482849315496777 7.816194373121671E-5 96-97 0.002657506086861368 3.9080971865608354E-5 0.0 3.4234149734835606 7.816194373121671E-5 98-99 0.002833370460256606 3.9080971865608354E-5 0.0 3.7270545843934046 7.816194373121671E-5 100-101 0.002872451432122214 3.9080971865608354E-5 0.0 4.076926985020264 7.816194373121671E-5 102-103 0.0029310728899206266 3.9080971865608354E-5 0.0 4.460115914162554 7.816194373121671E-5 104-105 0.003419585038240731 3.9080971865608354E-5 0.0 4.843343924276709 7.816194373121671E-5 106-107 0.0037126923272327935 3.9080971865608354E-5 0.0 5.233606509326674 7.816194373121671E-5 108-109 0.0037908542709640105 3.9080971865608354E-5 0.0 5.634108309005429 7.816194373121671E-5 110-111 0.003869016214695227 3.9080971865608354E-5 0.0 6.089206226380438 7.816194373121671E-5 112-113 0.003869016214695227 3.9080971865608354E-5 0.0 6.583111548817996 7.816194373121671E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 59.8223 1 GTATCAA 6995 0.0 52.902905 1 ATCAACG 8455 0.0 43.555744 3 TCAACGC 8540 0.0 42.913235 4 CAACGCA 8665 0.0 42.43149 5 AACGCAG 8980 0.0 40.87204 6 TATCAAC 9145 0.0 40.139305 2 GTACATG 11850 0.0 36.600418 1 ACGCAGA 10315 0.0 35.29459 7 TACATGG 12235 0.0 34.572758 2 CGCAGAG 10430 0.0 34.341125 8 ACATGGG 12610 0.0 32.412315 3 GCAGAGT 11255 0.0 30.185715 9 GTATAGG 900 0.0 27.764103 1 CATGGGA 7575 0.0 26.703283 4 CATGGGG 5450 0.0 25.98058 4 GAGTACT 5220 0.0 23.600029 12-13 GAGTACA 8955 0.0 23.518766 1 AGAGTAC 10920 0.0 23.35085 10-11 ATGGGAT 3485 0.0 23.216915 5 >>END_MODULE