FastQCFastQC Report
Thu 26 May 2016
SRR1364570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558790
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69550.2718081593253061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60710.23726058019610832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.14710077810214983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53700.067.0631261
GTATCAA99250.051.6641241
ATCAACG129350.039.695883
TCAACGC131550.039.032024
CAACGCA132750.038.7249835
TATCAAC132600.038.2287982
AACGCAG136050.038.0043456
GTACATG121400.032.709711
ACGCAGA159650.032.1255387
CGCAGAG161950.031.5958148
TACATGG125500.030.9462812
ACATGGG128950.029.1020643
GCAGAGT174400.028.5533499
GAGTACT106800.026.21047812-13
GTACTTT110050.025.40939714-15
CATGGGA75400.024.8853534
AGTACTT111800.024.31985512-13
AGAGTAC171450.023.80457910-11
CCAATAC19750.023.5251353
CAGAGTA179750.023.43355810-11
Produced by FastQC (version 0.11.5)