##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364570_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2558790 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.01047135560167 31.0 30.0 34.0 26.0 34.0 2 29.883136169830273 31.0 30.0 34.0 26.0 34.0 3 30.191504969145573 31.0 30.0 34.0 26.0 34.0 4 33.7634979814678 35.0 35.0 37.0 29.0 37.0 5 33.1509752656529 35.0 33.0 37.0 26.0 37.0 6 33.18442857756987 35.0 33.0 37.0 27.0 37.0 7 32.96418346171433 35.0 33.0 37.0 26.0 37.0 8 32.89054318642796 35.0 33.0 37.0 26.0 37.0 9 34.11031346847533 38.0 34.0 39.0 25.0 39.0 10-11 34.046759796622624 38.0 34.0 39.0 25.0 39.0 12-13 33.92263804376287 38.0 33.5 39.0 24.0 39.0 14-15 34.88365672837552 39.0 34.0 41.0 24.5 41.0 16-17 34.88808030358099 39.0 34.0 41.0 25.0 41.0 18-19 34.916214890631906 39.0 34.0 41.0 24.0 41.0 20-21 34.93089663473751 39.0 34.0 41.0 24.0 41.0 22-23 34.89069501600366 39.0 34.0 41.0 24.0 41.0 24-25 34.842615454961134 39.0 34.0 41.0 24.0 41.0 26-27 34.717273398754884 39.0 34.0 41.0 24.0 41.0 28-29 34.5640038846486 39.0 33.5 41.0 23.0 41.0 30-31 34.394536089323466 38.0 33.0 40.5 22.5 41.0 32-33 34.18069947123445 38.0 33.0 40.0 21.5 41.0 34-35 33.98781943809379 38.0 33.0 40.0 19.5 41.0 36-37 33.78198523520883 38.0 33.0 40.0 18.0 41.0 38-39 33.59758694539216 38.0 33.0 40.0 16.0 41.0 40-41 33.61328713962459 38.0 33.0 40.0 16.0 41.0 42-43 33.45949335428074 38.0 33.0 40.0 15.0 41.0 44-45 33.28077919641706 38.0 32.0 40.0 12.0 41.0 46-47 33.02301634757053 38.0 32.0 40.0 8.5 41.0 48-49 32.71622661492346 38.0 31.5 40.0 6.0 41.0 50-51 32.53956010458068 38.0 31.0 40.0 2.0 41.0 52-53 32.34829548341208 38.0 31.0 40.0 2.0 41.0 54-55 32.14681919969986 37.0 31.0 40.0 2.0 41.0 56-57 31.922814885160562 37.0 31.0 40.0 2.0 41.0 58-59 31.71495980522044 37.0 30.0 40.0 2.0 41.0 60-61 31.467920775053834 36.0 30.0 40.0 2.0 41.0 62-63 31.286913150356224 36.0 30.0 39.5 2.0 41.0 64-65 31.141312299954276 35.5 30.0 39.0 2.0 41.0 66-67 30.842667628058575 35.0 30.0 39.0 2.0 41.0 68-69 30.550331602046278 35.0 29.5 39.0 2.0 40.5 70-71 30.23884238253237 35.0 29.0 38.0 2.0 40.0 72-73 29.952987154084546 35.0 29.0 37.0 2.0 40.0 74-75 29.607167254835293 35.0 29.0 37.0 2.0 39.0 76-77 29.30470573982234 35.0 29.0 36.0 2.0 39.0 78-79 29.026312827547393 34.0 29.0 36.0 2.0 38.5 80-81 28.75637957784734 34.0 29.0 35.5 2.0 37.0 82-83 28.480683057226265 34.0 28.5 35.0 2.0 37.0 84-85 28.26360056901895 34.0 28.0 35.0 2.0 36.5 86-87 28.053210111029042 34.0 28.0 35.0 2.0 36.0 88-89 27.87760249961896 34.0 27.0 35.0 2.0 36.0 90-91 27.739372515915726 34.0 27.0 35.0 2.0 35.5 92-93 27.625242595132853 34.0 27.0 35.0 2.0 35.0 94-95 27.513365887782896 34.0 27.0 35.0 2.0 35.0 96-97 27.40289023327432 34.0 27.0 35.0 2.0 35.0 98-99 27.321125610151675 34.0 27.0 35.0 2.0 35.0 100-101 27.220054791522557 34.0 26.0 35.0 2.0 35.0 102-103 25.942495671782368 31.5 24.5 34.0 2.0 35.0 104-105 26.883364207301106 33.5 25.0 35.0 2.0 35.0 106-107 27.047593589157376 34.0 26.0 35.0 2.0 35.0 108-109 27.052156878837266 34.0 26.0 35.0 2.0 35.0 110-111 26.969276298562995 34.0 25.0 35.0 2.0 35.0 112-113 26.88303201904025 34.0 25.0 35.0 2.0 35.0 114-115 26.778608248429922 34.0 25.0 35.0 2.0 35.0 116-117 26.652882417079947 34.0 25.0 35.0 2.0 35.0 118-119 26.518163858698838 34.0 25.0 35.0 2.0 35.0 120-121 26.317761129283763 34.0 25.0 35.0 2.0 35.0 122-123 26.024187604297342 34.0 24.0 35.0 2.0 35.0 124-125 24.219891823869876 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7001987766840294 1101 2 0.7738032352823687 1101 3 0.6371098846358088 1101 4 0.6490077750505279 1101 5 0.6603992593798083 1101 6 0.7007169260491324 1101 7 0.7910596164896049 1101 8 0.9093108371045204 1101 9 1.0509375160230192 1101 10-11 1.053701997265165 1101 12-13 1.0008617927553871 1101 14-15 1.093359697220805 1101 16-17 1.102464588737348 1101 18-19 1.1425456789849875 1101 20-21 1.1352918306431121 1101 22-23 1.2430153335788177 1101 24-25 1.0929561000361332 1101 26-27 1.1721380773555694 1101 28-29 1.3389662267835831 1101 30-31 1.246875689413244 1101 32-33 1.2759197986401745 1101 34-35 1.330789390514063 1101 36-37 1.2799818541229726 1101 38-39 1.3546367829522126 1101 40-41 1.1642835660286863 1101 42-43 1.0742313711788185 1101 44-45 1.0555237573898424 1101 46-47 1.2448338133703416 1101 48-49 1.3058776508340273 1101 50-51 1.2979515764987326 1101 52-53 1.2111151997866827 1101 54-55 1.3039669710603832 1101 56-57 1.2391292208796187 1101 58-59 1.205297769105627 1101 60-61 1.2295398833052253 1101 62-63 1.3516225959379433 1101 64-65 1.1698772911339432 1101 66-67 1.3120449285246352 1101 68-69 1.300477302576109 1101 70-71 1.2919522444783453 1101 72-73 1.2281794895613025 1101 74-75 1.1641477594192118 1101 76-77 1.3015853405150963 1101 78-79 1.180440555499711 1101 80-81 1.0146926183651352 1101 82-83 1.2160939736324785 1101 84-85 1.250556392721382 1101 86-87 1.309646480734262 1101 88-89 1.3379714257086164 1101 90-91 1.2085982130725803 1101 92-93 1.2340958040418712 1101 94-95 1.2502240321818618 1101 96-97 1.3407598223876889 1101 98-99 1.2856072681491817 1101 100-101 1.2234318910951103 1101 102-103 1.1584389629960263 1101 104-105 1.3976266895071454 1101 106-107 1.3298940275755022 1101 108-109 1.2621267199652628 1101 110-111 1.2455687413041154 1101 112-113 1.2179320668758606 1101 114-115 1.3184200651640374 1101 116-117 1.2747831189784087 1101 118-119 1.2479452992755142 1101 120-121 1.2699197011913874 1101 122-123 1.232747635231977 1101 124-125 1.4295212495041696 1102 1 0.2938845642887422 1102 2 -0.4902259856041731 1102 3 0.1371018327033866 1102 4 0.17069205284727929 1102 5 0.35536564326826436 1102 6 0.3473817450092014 1102 7 0.2531084575340117 1102 8 0.42961442038396314 1102 9 0.6803519879730544 1102 10-11 0.631982389849135 1102 12-13 0.6572552531171354 1102 14-15 0.8170918215776695 1102 16-17 0.9371261377652829 1102 18-19 0.9250408767842373 1102 20-21 0.7153927368951685 1102 22-23 0.9517235408711571 1102 24-25 0.8234415355030009 1102 26-27 1.001388798427314 1102 28-29 0.5821368461014913 1102 30-31 0.8058570832338319 1102 32-33 0.9256422329907963 1102 34-35 0.908748341480063 1102 36-37 0.6508603595035041 1102 38-39 0.00724519939702617 1102 40-41 -0.24187102033788932 1102 42-43 0.03726438438426527 1102 44-45 -0.23634890454794544 1102 46-47 0.0380024832730399 1102 48-49 -0.2778315806076179 1102 50-51 -0.4195568278823103 1102 52-53 -0.7704255332442926 1102 54-55 -0.5685713624947297 1102 56-57 -0.588387297589108 1102 58-59 -0.5235830009868394 1102 60-61 -0.5748665296492916 1102 62-63 -0.5563843586714761 1102 64-65 -0.4135850850110927 1102 66-67 -0.5836552830575421 1102 68-69 -0.3408973967830988 1102 70-71 -0.9375154691090479 1102 72-73 -0.6645648152503902 1102 74-75 -0.5063419506349405 1102 76-77 -0.9819040590452417 1102 78-79 -0.7799755090378007 1102 80-81 -0.6860855655878986 1102 82-83 -0.9783825843670044 1102 84-85 -0.8909156361367465 1102 86-87 -0.6046914823189518 1102 88-89 -0.7395387359713581 1102 90-91 -0.7482030282246264 1102 92-93 -0.513382175335579 1102 94-95 -0.4739824498780081 1102 96-97 -0.7437243722340163 1102 98-99 -0.7369328453067823 1102 100-101 -0.5289369309184764 1102 102-103 -0.5515265464808152 1102 104-105 -0.4516279982007738 1102 106-107 -0.4533600295389739 1102 108-109 -0.36946897809913537 1102 110-111 -0.4317636280575208 1102 112-113 -0.30644466411303384 1102 114-115 -0.3563446559579866 1102 116-117 -0.5131087099488241 1102 118-119 -0.3347845629121302 1102 120-121 -0.06736515329324178 1102 122-123 -0.08673665686048793 1102 124-125 0.3137970403381303 1103 1 0.3634945619898673 1103 2 -0.11345293595878303 1103 3 0.3051358148682404 1103 4 0.3878794831519343 1103 5 0.37020110220247915 1103 6 0.32896716191175557 1103 7 0.6350891435592558 1103 8 0.6440046011029281 1103 9 0.8539392954423377 1103 10-11 0.7599788609056262 1103 12-13 0.7580743986221492 1103 14-15 0.8473483948139702 1103 16-17 0.8229820995696571 1103 18-19 0.8957409526127762 1103 20-21 0.8326321063173907 1103 22-23 0.9596591603834526 1103 24-25 0.7666342515028219 1103 26-27 0.7837619657613359 1103 28-29 0.8668045987289048 1103 30-31 0.8416704129301351 1103 32-33 0.9227780758713209 1103 34-35 0.8195513777096295 1103 36-37 0.9448428417386978 1103 38-39 0.9310006334896741 1103 40-41 0.9265382444684036 1103 42-43 0.8903039164432656 1103 44-45 0.9293094312729764 1103 46-47 0.9232605662177917 1103 48-49 0.8840295382235936 1103 50-51 0.8723165659079797 1103 52-53 0.831637406158066 1103 54-55 0.8291491064069945 1103 56-57 0.8847298128975325 1103 58-59 0.938915969666489 1103 60-61 0.890583234801877 1103 62-63 0.8892987329369539 1103 64-65 0.7509821433357473 1103 66-67 0.6660895247462797 1103 68-69 0.7187873400644271 1103 70-71 0.7694456164399526 1103 72-73 0.6099677326914339 1103 74-75 0.6477510319656687 1103 76-77 0.7649999663864833 1103 78-79 0.6197592626359523 1103 80-81 0.6484280852645732 1103 82-83 0.6317622275542014 1103 84-85 0.7082863628544906 1103 86-87 0.7501423296605871 1103 88-89 0.6940941372440079 1103 90-91 0.5781416845361882 1103 92-93 0.6393046768193571 1103 94-95 0.6141197608155302 1103 96-97 0.6304315634935769 1103 98-99 0.6583369207227179 1103 100-101 0.6722437297162216 1103 102-103 0.5134736937000781 1103 104-105 0.45344307818704266 1103 106-107 0.49606087717819847 1103 108-109 0.4861542141107691 1103 110-111 0.47822150646093675 1103 112-113 0.5195076102284446 1103 114-115 0.5513482521865463 1103 116-117 0.6612975714676956 1103 118-119 0.5120932570688588 1103 120-121 0.534222571061445 1103 122-123 0.5931039316513171 1103 124-125 0.9532943160006013 1104 1 0.31108226201038036 1104 2 0.5230887836515521 1104 3 0.2972908286002962 1104 4 0.10375052969174448 1104 5 0.46399530517052057 1104 6 0.49733914745247887 1104 7 0.6771011760899768 1104 8 0.42055788174565834 1104 9 0.6151847968954556 1104 10-11 0.6177805931958034 1104 12-13 0.6547499691484475 1104 14-15 0.670885118406737 1104 16-17 0.6301971893807661 1104 18-19 0.6198668160274323 1104 20-21 0.45173188415377297 1104 22-23 0.6128178994850444 1104 24-25 0.6933087032195289 1104 26-27 0.6017008454600017 1104 28-29 0.8142665991604474 1104 30-31 0.7619037180268435 1104 32-33 0.7685637599035076 1104 34-35 0.9310769478363525 1104 36-37 0.8776146059149426 1104 38-39 0.9833394782921374 1104 40-41 0.9571112481258481 1104 42-43 0.9984333667081842 1104 44-45 0.910908942726131 1104 46-47 0.9132417262635002 1104 48-49 0.9502668284135041 1104 50-51 0.8019115435184716 1104 52-53 0.9108078687858381 1104 54-55 0.5023513858483355 1104 56-57 0.6847556187734156 1104 58-59 0.8451184702059109 1104 60-61 0.9362686588440319 1104 62-63 0.9686168717705677 1104 64-65 0.9241106679189244 1104 66-67 1.0257569053200157 1104 68-69 0.6626877300494805 1104 70-71 0.24355506398776328 1104 72-73 0.2586198668207196 1104 74-75 0.7244019087660902 1104 76-77 0.8727058007120974 1104 78-79 0.8869560319400769 1104 80-81 0.8114004160379622 1104 82-83 0.7391016850381043 1104 84-85 0.8425545015549183 1104 86-87 0.8332167384895932 1104 88-89 0.8075538161456564 1104 90-91 0.7858785525086631 1104 92-93 0.7688578322619684 1104 94-95 0.7736995013049821 1104 96-97 0.8112405826971454 1104 98-99 0.8720718068039268 1104 100-101 0.8514158905371083 1104 102-103 0.7618127276346343 1104 104-105 0.7924907314839373 1104 106-107 0.8154817467063538 1104 108-109 0.7902279796242624 1104 110-111 0.7579320610974705 1104 112-113 0.7131213052569336 1104 114-115 0.7767436980987625 1104 116-117 0.8055490545563551 1104 118-119 0.822832497184045 1104 120-121 0.8875136360349778 1104 122-123 0.83515505592176 1104 124-125 1.1917945184518643 1105 1 0.1455160080732476 1105 2 -0.04042127143161167 1105 3 0.1895842422720797 1105 4 0.10684314672104023 1105 5 -0.07834565585696396 1105 6 0.37044235049680907 1105 7 0.3639962114283435 1105 8 0.4048987351187492 1105 9 0.5152149727472732 1105 10-11 0.48884162507944495 1105 12-13 0.5318504123256815 1105 14-15 0.6301053091507356 1105 16-17 0.4819068552769963 1105 18-19 0.5491630009842012 1105 20-21 0.61450059195856 1105 22-23 0.5298573058852654 1105 24-25 0.5928727029389762 1105 26-27 0.6014534034751193 1105 28-29 0.5848632491515531 1105 30-31 0.589168986600086 1105 32-33 0.6871768777540694 1105 34-35 0.7118112160230154 1105 36-37 0.8535872877978505 1105 38-39 0.8791367161414598 1105 40-41 0.6870178008528356 1105 42-43 0.6471181073508134 1105 44-45 0.6858297107260185 1105 46-47 0.6210049127029649 1105 48-49 0.7420890094588302 1105 50-51 0.42275408592369956 1105 52-53 0.5302918454085201 1105 54-55 0.5008323171566786 1105 56-57 0.5474638117618511 1105 58-59 0.29873655483857675 1105 60-61 0.24958583314464633 1105 62-63 0.4829616561600254 1105 64-65 0.28910276510799804 1105 66-67 0.47658347899068687 1105 68-69 0.5398229162344883 1105 70-71 0.5265518686097224 1105 72-73 0.5268688355405935 1105 74-75 0.46816521264670286 1105 76-77 0.4829500946216285 1105 78-79 0.3627644593214825 1105 80-81 0.3750456915598015 1105 82-83 0.4485281318070875 1105 84-85 0.4099719216663864 1105 86-87 0.5731322134819656 1105 88-89 0.5861808311993322 1105 90-91 0.43862349479123 1105 92-93 0.6123363413465306 1105 94-95 0.5235808368066301 1105 96-97 0.5044603134633014 1105 98-99 0.4195772425350732 1105 100-101 0.5313797773825577 1105 102-103 0.19626441609587886 1105 104-105 0.39029931219128855 1105 106-107 0.37932161569422007 1105 108-109 0.37033214340247156 1105 110-111 0.27743234899140745 1105 112-113 0.30257623150551893 1105 114-115 0.30588068504811616 1105 116-117 -0.09085561503437845 1105 118-119 0.0935270148026639 1105 120-121 0.22799788505589547 1105 122-123 0.2532731266898409 1105 124-125 0.7366642158614667 1106 1 0.015462601589824487 1106 2 -0.33041295334253107 1106 3 0.1826121103809939 1106 4 0.0683175070901143 1106 5 0.3668518611013951 1106 6 0.4391494315839566 1106 7 0.4931742938493926 1106 8 0.43803351661409806 1106 9 0.7462262147199823 1106 10-11 0.4943012706317802 1106 12-13 0.5347409901232467 1106 14-15 0.5692962285422709 1106 16-17 0.45106364874570914 1106 18-19 0.46446175762235953 1106 20-21 0.5457893267219731 1106 22-23 0.48007245484935623 1106 24-25 0.5325536699126445 1106 26-27 0.6965745178647467 1106 28-29 0.6898037254416494 1106 30-31 0.6994514501603248 1106 32-33 0.6332952423206848 1106 34-35 0.7672307774237268 1106 36-37 0.6694748379448825 1106 38-39 0.6799693618795715 1106 40-41 0.6046901417840402 1106 42-43 0.41246665536727534 1106 44-45 0.46874231388622434 1106 46-47 0.42004590565183975 1106 48-49 0.6902586114457918 1106 50-51 0.746749680851174 1106 52-53 0.5980243544086221 1106 54-55 0.6095112472863988 1106 56-57 0.17872459640702942 1106 58-59 0.62389450647985 1106 60-61 0.6009820838120099 1106 62-63 0.480659202407427 1106 64-65 0.6534745652797156 1106 66-67 0.455593609653306 1106 68-69 0.5120629257408744 1106 70-71 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-1.3710886432510137 2216 72-73 -1.3067788692143232 2216 74-75 -1.3339751069941528 2216 76-77 -1.415999287670104 2216 78-79 -1.4511778409553635 2216 80-81 -1.3454552500411516 2216 82-83 -1.2676021283751098 2216 84-85 -1.2691052424661073 2216 86-87 -1.3713745613594348 2216 88-89 -1.3376856899176452 2216 90-91 -1.3743128887219207 2216 92-93 -1.3460822346897459 2216 94-95 -1.1440458718913433 2216 96-97 -1.1779072592608593 2216 98-99 -1.2240630149726321 2216 100-101 -1.3358695633803706 2216 102-103 -1.2072085795571255 2216 104-105 -1.254953526071322 2216 106-107 -1.248417561278739 2216 108-109 -1.2156931364949912 2216 110-111 -1.2206437933636387 2216 112-113 -1.1839951216496942 2216 114-115 -1.3357169488284484 2216 116-117 -1.2908339339121753 2216 118-119 -1.2995606651106364 2216 120-121 -1.4000323136916073 2216 122-123 -1.3824444995386713 2216 124-125 -1.6987139625692222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82871.0 3 27876.0 4 13371.0 5 10481.0 6 11392.0 7 13436.0 8 15309.0 9 16778.0 10 18367.0 11 20087.0 12 22423.0 13 25678.0 14 29176.0 15 25367.0 16 17710.0 17 15049.0 18 13811.0 19 13213.0 20 13375.0 21 13666.0 22 15133.0 23 18691.0 24 24270.0 25 32036.0 26 40033.0 27 45320.0 28 51787.0 29 61803.0 30 71433.0 31 83607.0 32 101181.0 33 126917.0 34 163600.0 35 223139.0 36 358391.0 37 531378.0 38 189999.0 39 636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26216743187459 20.047078282804573 13.967587419919191 23.723166865401648 2 15.482630462054331 20.77462394334822 39.00691342392303 24.73583217067442 3 18.822021346026833 25.53382653519828 29.543456086665966 26.100696032108928 4 12.811963695498163 17.085147991191842 36.78970154526112 33.31318676804888 5 13.068247101168911 38.80513836618089 33.97957628410303 14.147038248547164 6 31.565505570992542 37.58092692249071 16.96465907714193 13.88890842937482 7 27.99639351771155 32.89558944568508 21.049733343859405 18.058283692743966 8 26.2697512849645 35.718788680352894 19.27675385782475 18.73470617685786 9 26.5018243724909 15.792624970297279 19.84544422766668 37.86010642954514 10-11 25.114293016890123 26.953318268827182 27.02116326021759 20.91122545406511 12-13 25.948690592037643 24.280167579207358 27.74571184036205 22.025429988392954 14-15 22.86534316324184 25.471301957429482 25.841125266459002 25.82222961286968 16-17 22.15142246416898 28.774848497224166 26.526532257639033 22.547196780967816 18-19 22.092503377640313 26.783583632406433 29.399852818126426 21.724060171826824 20-21 22.831679012519373 25.997594923154004 29.59735480628462 21.573371258042002 22-23 22.524748025433897 26.001938416204535 29.335056804192604 22.138256754168964 24-25 22.2180405582326 26.283594980439972 29.438738622552062 22.05962583877536 26-27 21.99316864611789 26.45262018375873 29.465137819047282 22.089073351076095 28-29 21.960594656067908 26.477006710202872 29.5715943864092 21.99080424732002 30-31 22.123640470691225 26.277322484455546 29.453374446515735 22.145662598337495 32-33 21.98214781205179 26.33088295639736 29.297539071201623 22.389430160349228 34-35 22.360764267485802 26.38217673197097 29.218263319772237 22.03879568077099 36-37 22.557790987146266 26.029744527686915 29.068485495097292 22.343978990069523 38-39 22.258000851965186 26.376001938416206 29.07954541013526 22.286451799483352 40-41 22.424524873084543 26.218036650135417 29.076458013357875 22.280980463422164 42-43 22.33487312362484 26.30647688946729 28.78075183973675 22.577898147171123 44-45 22.38812094779173 26.409924221995553 28.735496074316373 22.466458755896344 46-47 22.72755872893047 26.782600369705996 28.083586382626162 22.406254518737374 48-49 22.64050586409983 26.902266305558488 27.777328346601323 22.679899483740364 50-51 22.693640879798828 26.91430850407976 27.633085879084625 22.75896473703679 52-53 23.026528163702377 26.92034125504633 27.51362948893813 22.539501092313163 54-55 22.74295663184552 26.892359279190554 27.595347801109117 22.769336287854806 56-57 22.60188670359196 26.979572143167164 27.535777036527353 22.882764116713524 58-59 22.72081276598489 26.698448882734017 27.597494654166642 22.983243697114453 60-61 22.74059223304765 26.844875898373843 27.727226540669612 22.687305327908895 62-63 22.63005170412578 26.859961153513968 27.82279905736696 22.6871880849933 64-65 22.385309735135806 26.68952388694156 27.840793846137014 23.08437253178562 66-67 22.33704211756338 26.978689146041685 27.616353823486882 23.067914912908055 68-69 22.57338870587049 26.68949516949323 27.820576878246527 22.916539246389746 70-71 22.409947670578674 26.724526045513702 27.833858972404922 23.0316673115027 72-73 22.59018911282286 26.964717698599728 27.36375396183352 23.081339226743893 74-75 22.532613453391487 27.131716143757135 27.288471759758632 23.04719864309275 76-77 22.738520941538773 26.85212541865491 27.5086662055112 22.900687434295115 78-79 22.628273519905893 26.965636101438573 27.303901453421343 23.102188925234195 80-81 22.464200838756145 27.09006096243184 27.374375143866885 23.07136305494513 82-83 22.745711840362045 27.170224989155027 27.20291622212061 22.881146948362314 84-85 22.61807338624897 27.055678660616934 27.1823596309193 23.1438883222148 86-87 22.611136513742824 26.94715080174614 27.314805044571848 23.12690763993919 88-89 22.63137109533998 27.06309927861359 27.06870747807477 23.236822147971658 90-91 22.552230762286378 27.221705267609014 27.216683360761674 23.009380609342937 92-93 22.597271343241758 27.117206318834157 27.245609787506037 23.039912550418048 94-95 22.45297582060271 26.778965839322495 27.378741514543986 23.389316825530816 96-97 22.71055170009427 26.677464390181367 27.205827174930935 23.406156734793424 98-99 22.328240470961774 26.858918215219653 27.035181044593713 23.777660269224864 100-101 22.292715202800142 26.766914472280344 27.294618493734774 23.64575183118474 102-103 22.372664423418883 26.616897048995813 27.26177216575022 23.748666361835085 104-105 22.271913677949343 26.56843664388246 27.27365278119736 23.885996896970834 106-107 22.25458827373199 26.722588481645925 27.286320552488302 23.73650269213378 108-109 22.29329096955983 26.58586675733452 27.001141164378478 24.11970110872717 110-111 22.250371416294403 26.620431208309096 27.1649080530947 23.964289322301802 112-113 22.277717202271386 26.6420261139054 27.281820704317273 23.79843597950594 114-115 22.255011157617467 26.809996130983787 27.008507927575142 23.926484783823604 116-117 22.001531974097134 26.7356641224954 27.16561343447489 24.09719046893258 118-119 21.949534741029943 26.71420866894118 27.055229229440474 24.281027360588403 120-121 22.003205845323336 26.80538861836977 27.16317862123639 24.0282269150705 122-123 21.98731959639227 26.82611784040357 26.988584900959385 24.197977662244778 124-125 21.876439917545774 26.642239406705077 27.021399766092852 24.459920909656297 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 229.0 1 183.0 2 233.0 3 368.5 4 296.0 5 238.5 6 469.0 7 1104.0 8 1869.0 9 2218.0 10 1841.5 11 1314.5 12 1272.0 13 1405.0 14 1600.5 15 1856.0 16 2224.0 17 2580.5 18 2891.5 19 3213.0 20 3589.5 21 4248.0 22 5214.0 23 6376.5 24 7863.0 25 10012.5 26 13419.0 27 17717.5 28 21254.5 29 25415.5 30 31408.5 31 37561.5 32 44641.0 33 50665.5 34 55672.5 35 60313.0 36 65078.0 37 70841.5 38 73577.0 39 75275.0 40 76381.5 41 77124.0 42 78039.0 43 80057.5 44 82806.5 45 84960.5 46 85697.0 47 84968.0 48 87307.5 49 88314.5 50 85861.5 51 84344.0 52 84505.5 53 86370.0 54 87276.0 55 83489.5 56 77865.0 57 73614.0 58 70480.5 59 64002.5 60 53538.0 61 43253.0 62 34771.0 63 26532.0 64 18780.0 65 13579.5 66 9790.5 67 7319.0 68 5355.0 69 3783.5 70 2808.5 71 2048.0 72 1538.5 73 1349.5 74 1130.0 75 817.5 76 572.0 77 330.0 78 214.0 79 125.5 80 78.0 81 60.0 82 41.5 83 26.5 84 14.5 85 6.5 86 6.0 87 6.5 88 6.5 89 4.0 90 1.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10247030823162512 2 0.0 3 0.0 4 0.09860129201692987 5 0.0 6 0.0 7 0.0025011821993989347 8 0.006213874526631728 9 0.003986259130292052 10-11 6.252955498497337E-4 12-13 0.0 14-15 1.9540485932804177E-5 16-17 3.32188260857671E-4 18-19 0.001993129565146026 20-21 0.0014069149871619008 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.9540485932804177E-5 52-53 0.0 54-55 0.0 56-57 0.007835734859054474 58-59 8.402408951105796E-4 60-61 0.0 62-63 0.0 64-65 2.735668030592585E-4 66-67 0.0 68-69 1.9540485932804177E-5 70-71 0.0 72-73 0.0 74-75 0.0011724291559682507 76-77 0.0 78-79 0.0 80-81 3.9080971865608354E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.001426455473094705 90-91 3.9080971865608354E-5 92-93 0.002286236854138089 94-95 0.0 96-97 0.008832299641627487 98-99 0.004337987877082527 100-101 0.0025011821993989347 102-103 0.0 104-105 0.0 106-107 0.0015241579027587258 108-109 0.0 110-111 1.3678340152962926E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010356457544386214 122-123 0.0045920141942089815 124-125 0.08675975754165055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2558790.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.58886231929254 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95411400866989 35.933462635125245 2 12.853586133773446 12.49082253926171 3 5.059410965072771 7.374930683959194 4 2.530085631051567 4.917359295327398 5 1.4419237159417846 3.503071645440902 6 0.8990640047358568 2.6210698285405396 7 0.5969400420398683 2.0303246260883503 8 0.43332945875449014 1.6843988328252382 9 0.31776366882577123 1.38957976391838 >10 1.7048637533882873 15.314786243258341 >50 0.13458828170393392 4.522449860180007 >100 0.06987876161590965 5.954137611231743 >500 0.0033993841074706917 1.0647708303380066 >1k 8.903148852882176E-4 0.6865658044695055 >5k 1.6187543368876683E-4 0.5122698000355013 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6955 0.2718081593253061 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6071 0.23726058019610832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3764 0.14710077810214983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.9080971865608354E-5 0.0 0.0 0.0 0.0 3 3.9080971865608354E-5 0.0 0.0 0.0 0.0 4 3.9080971865608354E-5 0.0 0.0 0.0 0.0 5 3.9080971865608354E-5 0.0 0.0 0.0 0.0 6 3.9080971865608354E-5 0.0 0.0 0.0 0.0 7 7.816194373121671E-5 0.0 0.0 0.0 0.0 8 1.1724291559682506E-4 0.0 0.0 0.0 0.0 9 1.1724291559682506E-4 0.0 0.0 0.0 0.0 10-11 1.1724291559682506E-4 0.0 0.0 0.0 0.0 12-13 1.3678340152962923E-4 0.0 0.0 0.0 0.0 14-15 2.344858311936501E-4 0.0 0.0 1.3678340152962923E-4 0.0 16-17 2.344858311936501E-4 0.0 0.0 2.1494534526084592E-4 0.0 18-19 2.7356680305925846E-4 0.0 0.0 2.7356680305925846E-4 0.0 20-21 3.1264777492486683E-4 0.0 0.0 3.1264777492486683E-4 0.0 22-23 3.517287467904752E-4 0.0 0.0 4.2989069052169185E-4 0.0 24-25 4.298906905216919E-4 0.0 0.0 6.057550639169295E-4 0.0 26-27 4.4943117645449606E-4 0.0 0.0 9.184028388417964E-4 0.0 28-29 7.034574935809504E-4 0.0 0.0 0.0015632388746243341 0.0 30-31 7.425384654465587E-4 0.0 0.0 0.0032241801789126895 0.0 32-33 7.425384654465587E-4 0.0 0.0 0.007366763196667174 0.0 34-35 8.402408951105795E-4 0.0 0.0 0.01385420452635816 0.0 36-37 0.0010356457544386214 0.0 0.0 0.02483595762059411 0.0 38-39 0.0010942672122370338 0.0 0.0 0.043321257313026856 0.0 40-41 0.0010942672122370338 0.0 0.0 0.06522614204370034 0.0 42-43 0.0011528886700354463 0.0 0.0 0.08675975754165055 0.0 44-45 0.0012896720715650757 0.0 0.0 0.11241641557142243 0.0 46-47 0.0014069149871619008 0.0 0.0 0.1441306242403636 0.0 48-49 0.0014850769308931175 0.0 0.0 0.17895177017262065 0.0 50-51 0.0014850769308931175 0.0 0.0 0.21955689994098773 0.0 52-53 0.0014850769308931175 0.0 0.0 0.2582666025738728 0.0 54-55 0.0014850769308931175 0.0 0.0 0.2993797849764928 0.0 56-57 0.0014850769308931175 0.0 0.0 0.3493252670207403 0.0 58-59 0.0014850769308931175 0.0 0.0 0.40751683412863116 1.9540485932804177E-5 60-61 0.0015046174168259216 0.0 0.0 0.47528323934359595 3.9080971865608354E-5 62-63 0.0015241579027587258 0.0 0.0 0.5448669097503116 3.9080971865608354E-5 64-65 0.0015241579027587258 0.0 0.0 0.6143528777273632 3.9080971865608354E-5 66-67 0.0016023198464899424 0.0 0.0 0.6934918457552203 7.816194373121671E-5 68-69 0.0016023198464899424 0.0 0.0 0.7873643401764115 7.816194373121671E-5 70-71 0.0016414008183555507 0.0 0.0 0.8969864662594429 7.816194373121671E-5 72-73 0.0017391032480195718 0.0 0.0 1.0114937138256754 7.816194373121671E-5 74-75 0.0017977247058179842 0.0 0.0 1.1260986638215718 7.816194373121671E-5 76-77 0.0017977247058179842 0.0 0.0 1.2553394377811387 7.816194373121671E-5 78-79 0.0018172651917507884 0.0 0.0 1.401814920333439 7.816194373121671E-5 80-81 0.002110372480742851 0.0 0.0 1.5686515892277209 7.816194373121671E-5 82-83 0.0021494534526084593 0.0 0.0 1.7500654606278747 7.816194373121671E-5 84-85 0.0021494534526084593 0.0 0.0 1.9369897490610797 7.816194373121671E-5 86-87 0.0021494534526084593 0.0 0.0 2.1274117844762563 7.816194373121671E-5 88-89 0.0021494534526084593 0.0 0.0 2.343607720836802 7.816194373121671E-5 90-91 0.0023643987978693055 0.0 0.0 2.593745481262628 7.816194373121671E-5 92-93 0.002423020255667718 0.0 0.0 2.8607076000765987 7.816194373121671E-5 94-95 0.002423020255667718 0.0 0.0 3.1331215144658215 7.816194373121671E-5 96-97 0.002423020255667718 0.0 0.0 3.407821665709183 7.816194373121671E-5 98-99 0.002559803657197347 0.0 0.0 3.7119107077954814 7.816194373121671E-5 100-101 0.0027161275446597804 0.0 0.0 4.062115296683198 7.816194373121671E-5 102-103 0.0028138299743238017 0.0 0.0 4.446320331093993 7.816194373121671E-5 104-105 0.0031655587211142765 0.0 0.0 4.829841448497142 7.816194373121671E-5 106-107 0.003243720664845493 0.0 0.0 5.218775280503675 7.816194373121671E-5 108-109 0.0032632611507782978 0.0 0.0 5.622071369670821 7.816194373121671E-5 110-111 0.0033414230945095144 0.0 0.0 6.0784980400892605 7.816194373121671E-5 112-113 0.0033609635804423186 3.9080971865608354E-5 0.0 6.572930955647005 7.816194373121671E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5370 0.0 67.063126 1 GTATCAA 9925 0.0 51.664124 1 ATCAACG 12935 0.0 39.69588 3 TCAACGC 13155 0.0 39.03202 4 CAACGCA 13275 0.0 38.724983 5 TATCAAC 13260 0.0 38.228798 2 AACGCAG 13605 0.0 38.004345 6 GTACATG 12140 0.0 32.70971 1 ACGCAGA 15965 0.0 32.125538 7 CGCAGAG 16195 0.0 31.595814 8 TACATGG 12550 0.0 30.946281 2 ACATGGG 12895 0.0 29.102064 3 GCAGAGT 17440 0.0 28.553349 9 GAGTACT 10680 0.0 26.210478 12-13 GTACTTT 11005 0.0 25.409397 14-15 CATGGGA 7540 0.0 24.885353 4 AGTACTT 11180 0.0 24.319855 12-13 AGAGTAC 17145 0.0 23.804579 10-11 CCAATAC 1975 0.0 23.525135 3 CAGAGTA 17975 0.0 23.433558 10-11 >>END_MODULE